SERBIAN GENETICS SOCIETY

 

SECOND CONGRESS OF SERBIAN GENETICISTS

 

Sokobanja, Serbia, Serbia, November 10-13, 1999.

 

 


 


 


 

Chairmanship

Katarina BOROJEVIĆ

Janko DUMANOVIĆ

Ivan MIHALJEV

Bogoljub SOLDATOVIĆ

Aleksandar TUCOVIĆ

Slobodan ŽIVKOVIĆ

 

 

Program Committee

Kosana KONSTANTINOV, president

Marko ANĐELKOVIĆ

Nada BARJAKTAROVIĆ

Janoš BERENJI

Vojislav GUZINA

Mile IVANOVIĆ

Vasilije ISAJEV

Marija KRALJEVIĆ-BALALIĆ

Dragoslav MARINKOVIĆ

Draga SIMIĆ

Ljubiša TOPISIROVIĆ

Mladen VUJOŠEVIĆ

 

 

Organizing Committee

Vasilije ISAJEV, president
Jelena KNEŽEVIĆ-VUKČEVIĆ, vice-president
Marina STAMENKOVIĆ-RADAK, vice-president
Janoš BERENJI
Živorad GAJIĆ
Miodrag DIMITRIJEVIĆ
Selma KANAZIR
Desimir KNEŽEVIĆ
Miroslava MICIĆ
Snežana MLADENOVIĆ DRINIĆ
Sasa ORLOVIĆ
Slaven PRODANOVIĆ
Branka VASILJEVIĆ

 

 

Secretariat

Mirjana ŠIJAČIĆ-NIKOLIĆ, president
Dražen JELOVAC, vice-president
Jelena BEAGOJEVIĆ
Veselinka ĐORĐEVIĆ
Marina ĐURIŠIĆ
Tatjana TERZIĆ

 



 

SERBIAN GENETICS SOCIETY

 

SECOND CONGRESS OF SERBIAN GENETICISTS

 

Sokobanja, Serbia, Serbia, November 10-13, 1999.

 

CONTENTS

 

 

Kosana Konstantinov

Genetics at third milenium beginning

 

 

Plenary lectures

 

Lj. Topisirović. M. Kojić, Dj. Fira, N. Miladinov, I. Strahinić, O. Gajić, S. Arsenijević i I. Spasojević

Molecular genetics of lactic acid bacteria is a basis for development of modern dairy biotechnology

 

Drinić Mladenović Snežana, Jelovac Dražen i Konstantinov Kosana

Molecular biology and information technology in plant breeding

 

Selma Kanazir, Sabera Ruždijić, Ljubisav Rakić

Modulation of gene expression byantisense oligonucleotides

 

Mladen Vujošević i Jelena Blagojević

Populational and evolutionary aspects of B-hromosomes - A case of yellow-necked mice

 

Marko Anđelković i Marina Stamenković-Radak

Population genetic aspect of stress

 

Draga Simić, Jelena Knežević-Vukčević

Plant antimutagens and cancer prevention

 

Roy Forster

The use of transgenic animals in carcinogenicity testing

 

Drinić Goran i Babić Milosav

Genetics and breeding of maize - achievements and challenges

 

Dragan Škorić

Achievements of sunflower breeding at the end of the 20th century

 

M. Ivanović, Violeta Anđelković

Efficiency of S1, HS and FS methods of recurrent selection in synthetic populations of narrow and broad genetic base of maize (Zea mays L.)

 

V.Isajev, A.Tucović

Gene pool conservation and tree breeding

 

Živoslav Marković, Jasmina Zdravković, Mirjana Mijatović

New approach in tomato breeding (Lycopersicon esculentum Mill.)

 

Desimir Knežević, Novo Pržulj

Contribution of genetics in breeding of small grains

 

Romac S.

The expansion of trinucleotide repeats as a genetical cause of neurological and psychiatric disease

 

Milena Stevanović i Marija Guc-Šćekić

Application of molecular techniques in diagnostics of chromosomal disorders

 

 

Sekcija 1. Molecular genetics

 

Goran Ljubijankić

Heterodimeric penicillin G amidases - molecular mechanizam of posttranslational maturation of inactive precursor and its biotechnological impication

 

Nataša Miladinov, Irena Spasojević i Ljubiša Topisirović

Structural analysis of the prt genes regulatory region in natural isolate Lactococcus lactis subsp. lactis BGIS29

 

Zvezdana Popović, Aleksandra Đurković, Jelena Zarić i Marina Lušić

Induction of albumin synthesis in Belgrade laboratory (b/b) rat reticulocytes

 

Stamenković Gorana, Laketa Danijela, Gudurić Jasenka, Veljković Emilija, Krtolica-Zikić

Koviljka i Dimitrijević Bogomir

HCV genotype detection by SSCP in Yugoslav population

 

Vesna Lazić-Jančić, D. Kovačević i S.A. Quarrie

BSA with candidate drought-induced genes

 

Milena Stevanović, Nikola Arsić, Tamara Rajić, Slavica Stanojčić, Tomislav Terzin, Vesna

Verbić, Maja Vujić, Nebojša Mirković i Danica Grujić

SOX genes: Brain development and sex determination

 

Nikola Tanić, Nasta Dedović, Mladen Vujošević i Bogomir Dimitrijević

Detection of b chromosome specific dna markers in populations of yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)

 

Branka Vasiljević, Milorad Kojić,  Milojević, Sandra Vajić, Marijana Miljković,

Tatjana Ilić

Control of resistance genes in antibiotic-producing actinomycetes

 

Jelena Brkljačić, Jelena Avramović, Paja Šijačić, Svetlana Radović, Vesna Maksimović

The isolation of buckwheat genomic clone containing the gene for metalothionein-like protein

 

Sandra Vajić, Milorad Kojić, Branka Vasiljević

Comparative study of Micromonospora strains producing aminoglycoside antibiotics

 

Marijana Vučinić, Z. Gajić i P. Stojić

The application of genetic markers in prevention of dairy cattle mastitis

 

Nataša Grujičić, Sonja Pavlović, Sanja Pavlović, Zvezdana Popović

Functional analysis of the rat βbminy - globin gene promoter

 

Tatjana Ilić, Milorad Kojić, Goran Ljubijankić, Branka Vasiljević

Synthesis and secretion of the sgm methylase in Saccharomyces cerevisiae

 

Radmila Janjušević, Mirjana Lilić, Milija Jovanović, Goran Jovanović i Dragutin Savić

The regulation of cls gene expression in bacteria Escherichia coli K12

 

Milija Jovanović, Mirjana Lilić, Radmila Janjušević, Goran Jovanović i Dragutin Savić

CysB activator acts as a repressor of the HslJ expression through N-acetyl serin modulated DNA binding in the hslJ locus

 

Milan Kojić, Slavica Arsenijević, Olivera Gajić, Vanesa Munćan i Ljubiša Topisirović

Construction of shuttle vector pA13

 

Marija Mijatović, Jelena Stanković, Marijana Petakov, Gordana Jovčić, Jelena Zarić, Zvezdana Popović

Large scale of isolation of rat erythroid progenitors for gene therapy

 

Marijana Miljković, Nataša Milojević, Milorad Kojić, Branka Vasiljević

Two-plasmid system for isolation of regulatory and gentamicin sensitive mutants of the sgm gene

 

Tatjana Mitrović i Zvezdana Popović

Promoter sequences required for regulated expression of the rat βbminy - globin gene in rel cells

 

Snežana Mladenović Drinić i Kosana Konstantinov

Polymorphism of PCR based markers and heterosis in maize

 

Pavković, N., Todorović, V., Glišin, V., Ljubijankić, G.

Yeast shuttle expression vector with dominant selective marker

 

Tamara Rajić, Nikola Arsić, Nebojsa Mirković. Milka Sokolović and Milena Stevanović

Localisation of control elements responsible for SOX3 gene activation by retinoic acid

 

Spasić M., Glišin V., Ljubijankić G.

Molecular-genetic analysis of temperature-dependent posttranslational maturation of E. coli pac

 

Irena Spasojević, Nataša Miladinov i Ljubiša Topisirović

Molecular analysis of the prt gene regulatory region of natural isolate Luctobacillus paracasei subsp. paracasei BGHN14

 

Slavica Stanojčić, Maja Vujić, Nikola Arsić, Tamara Rajić i Milena Stevanović

Preliminary characterisation of the three novel human SOX genes

 

Slavica Stanojčić, Jelena Đurović, Nikola Arsić, Nebojsa Mirković, Vesna Verbić, Milena Stevanović

Genotyping the bovine traits of economic interest

 

Ivana Strahinić, Milan Kojić i Ljubiša Topisirović

Analiza konjugacionih svojstava plazmida Lactococcus lactis subsp. lactis biov. diacetylactis S50

 

Tomislav Terzin, Milka Sokolović, Ana Basić i Milena Stevanović

Cloning of mole rats Sox2 gene using PCR method

 

Đorđe Fira i Ljubiša Topisirović

Biochemical and genetic characterization of staphylococcal extracellular proteinases

 

Ševo M., Glišin V. i Ljubijankić G.

Mechanism of posttranslational processing of the heterodimeric PAC

 

Paja Šijačić, Jelena Brkljačić, Svetlana Radović, Vesna Maksimović

The isolation of genomic clone containing the gene for a subunit of buckwheat seed storage protein

 

 

Sekcija 2. Population and Evolution Genetics

 

Svetlana Fišter

Karyotype analisis and chromosome number variability in the fish species Cyprinus carpio L. i Carassius auratus gibelio B. (Cyprinidae, Pisces)

 

Boro P. Pavlović

The inclusion of population or species chromosome variants in the combination potential of an individual

 

Goran Živanović i Marko Anđelković

Genetic loads and coadaptivity of chromosomal inversion polymorphism in a Drosophila subobscura

 

Jelena Blagojević i Mladen Vujošević

Does the presence of B chromosomes affect taxonomic determination in the genus Apodemus (Rodentia, Mammalia)?

 

S. Bojović, Ph. Heizmann, M. Barbero

Fraxinus ornus L., Sexual polymorphisme and RAPD markers

 

Ljiljana Vapa, Dragana Obreht, Milan Vapa*, Deana Demjen

Intra-and inter-population variability in hare (Lepus europaeus PALLAS)

 

Dimitrijević Miodrag, Knežević Desimir, Petrović Sofija

Gliadin allelic variation and diversity of agronomic properties in wheat

 

Đorđević Radiša, Zdravković Milan, Zečević Bogoljub, Sretenović-Rajačić Tatjana

"Afila" gene effects on absolute seed mass in peas (Pisum sativum L.)

 

Jelena Živanov-Čurlis, Stevo Najman, Estera Mrčarica

Influence of antioxidants on fertility and fecundity of D. melanogaster

 

Gordan Zec, Radmila Todorović, Petar Mišić, Slavica Čolić

Variability and correlation analysis of fruit characteristic of selected vineyard peach (Prunus persica L. Batsch.)

 

Mirjana Ivančević, Tatjana Sretenović-Rajičić, Nenad Pavlović, Jasmina Zdravković

Correlation analysis of some quantitative traits of autochtonous cabbage populations (Brassica oleracea var. capitata L.)

 

Janković Dragan, Janković Slađana

Atypical phenomena in walnut organogenesis

 

Slađana Janković

Growth habit forms in P. communis L. population in the Ibar - Kolasin region

 

Krajinčanić, B., Krajinčanić-Suzović V., Cukić R., Ninković D.

Population genetics investigations of PTC tasting sensibility from some localities in Serbia

 

Krajinčanić-Suzović V., Krajinčanić B., Čukić R., Gašić-Marušić R.

Frequency of hand clasping from some localities in Serbia

 

Milankov V., Vujić A., Simić S., Ludoški J.

Genetic differentiation in populations of Merodon avidus (ROSSI, 179) (Diptera: Syrphidae)

 

Milankov V., Vujić A., Simić S.

Evolutionary relationships among the members of the aeneus group species of genus Merodon (Diptera: Syrphidae)

 

Olivera Milošević, Dragoslav Marinković, Slobodan Arsenijević

Variability of frequency of SCEs and pri in peripheral blood lymphocytes in control sample of yugoslav population

 

Stevo Najman, Jelena Živanov-Čurlis, Estera Mrčarica

Influence of antioxidants on preadult developmental duration of D. melanogaster

 

Dragana Obreht, Saša Orlović, Joška Erdesi, Ljiljana Vapa

Allozyme variability in Quercus robur L. and identification possibility within the population

 

Boro P. Pavlović i Nevenka Pavlović

Distribution of the known diploid chromosome numbers of some eutherian genera presented in the faunas of continents and oceans

 

Petrović Sofija, Dimitrijević Miodrag, Mladenov Novica i Kraljević-Balalić Marija

Ecological stability of yield components in wheat

 

Vuk Savković, Marko Anđelković

Monitoring of temporal variability of chromosomal arangements in Drosophila subobscura

 

Tatjana Sretenović Rajičić, Mirjana Ivančević, Jasmina Zdravković, R. Đorđević, M. Damjanović

Reactivity of cabbage (Brassica oleracea var. capitata) on paraquat in vitro

 

Z. Stanimirović, Jevrosima Stevanović, D. Pejović, D. Popesković

Biodiversity of the honeybee Apis mellifera, Linne (1758) - cytogenetic aspects

 

Z. Stanimirović, B. Soldatović, Jevrosima Stevanović

The investigations of chromosome polymorphysm in the natural populations of the species Mus musculus, Linne (1758), on the territory of the Ex Yugoslavia

 

Milica Strnać, Zvonko Magić, Cedomir Radojičić i Dragoslav Marinković

The effect of trauma on the haptoglobin, α2- macroglobulin, IL-1, IL-6 I TNF concentrations in AO and DA inbred rats

 

IvanaTomišić, Marina Stamenković-Radak, Tatjana Terzić i Marko Anđelković

Genetic variability of morphometrical traits in a D. subobscura population across different developmental temperatures

 

 

Sekcija 3. Mutagenesis and genotoxicology

 

Gordana Joksić, Miroslava Stanković

Micronucleus assay in to human biomonitoring

 

Miroslava Veličković, Jelena Blagojević i Mladen Vujošević

The effects of disturbed environment on natural populations of rodents

 

Branka Vuković-Gačić, Tatjana Stević, Jelena Knežević-Vukčević, Dragana Mitić, Dejan Brkić, Jelena Blagojević, Marko Anđelković, Draga Simić

Comparative study of antigenotoxic effect of sage in prokaryotic and eukaryotic test-systems

 

Olivera Milošević, Darko Grujičić, Dragoslav Marinković, Slobodan Arsenijević, Smilja Banković, Aleksandar Živanović, Aleksandra Dimitrijević

Effect of different doses of gestagens on micronucleus frequency in human peripheral blood lymphocytes

 

Ninoslav Đelić, Bogosav Soldatović, Marko Anđelković

Evaluation of genotoxic and mitogenic effects in cultures of human lymphocytes treated with insulin

 

Ninoslav Đelić, Bogosav Soldatović, Dijana Đelić

Influence of cloprostenol on cell cycle kinetics in cultured human lymphocytes

 

Biljana Burić, Z. Stanimirović, Biljana Marković

Investigation of genotoxic effects of CARISOLV™ gel

 

Gordana Joksić, Miroslava Stanković

Chromosome aberrations monitored in radiation workers

 

Dubravka Jovičić, Snežana Milačić, Radomir Kovačević, Mitar Novaković

Analysis of chromosomal status of subjects occupationally exposed to radionuclides

 

Jelena Knežević-Vukčević, Tatjana Stević, Dragana Mitić, Branka Vuković-Gačić, Draga Simić

Molecular mechanisms of mutagenesis inhibition by ethereal oil of sage (Salvia officinalis L.)

 

Biljana Marković, Z. Stanimirović, Jevrosima Stevanović, N. Jovanović

Comparative analysis of genotoxic effects of Gastrogala 10, Tiamulina S i Carbadoxa in vitro

 

Dragana Mitić, Tanja Berić, Branka Vuković-Gačić, Jelena Knežević-Vukčević, Ratko M. Jankov, Draga Simić

Antimutagenic potential of rosmanol-9-ethyl ether, osaine and pomiferine

 

Danica Mićanović, Penny Hirsch, Philip Curnow, Vera Raičević i Desimir Knežević

Colonisation of wheat by Pseudomonas and marker genes

 

D. Pejović, Z. Stanimirović, B. Soldatović

Analysis of genotoxic and mutagenic effect of Apitol®

 

Z. Stanimirović, Jevrosima Stevanović, M. Kulić, D. Pejović

Investigations of cytotoxic and genotoxic effects of Levamisole® in vivo

 

Jevrosima Stevanović, Z. Stanimirović, D. Pejović

Cytogenetic effect of Fumagillin-et® on human lymphocytes in vitro

 

 

Sekcija 4. Breeding of organisms

 

Gordana Radović, Dražen Jelovac, Jasmina Muminović

Breeding potential of early maturity local maize populations  

 

Z. Tomić, Lugić Z., Sokolović D.

Genetic potential of seed germination in forage crops cultivars in the fifth year of their life

 

Branislav Dozet, Dragan Škorić, Radovan Marinković

Breeding sunflower for resistance to downy mildew (Plasmopara halstedi (Farl.) Berl. et Toni) and broomrape (Orobanche cernua Loefl./Orobanche cumana Wallr.)

 

Aleksandar Tucović, Vasilije Isajev i Milan Mataruga

Genetics of the juvenile phase of tree growth and the aspects of short-rotation plantation establishment

 

Sasa Orlović, Joška Erdeši, Srbislav Radivojević, Zoran Obućina, Gojko Janjatović

Strategy and previous results of pedunculate oak (Quercus robur L.) breeding in Yugoslavia

 

M. Mitrović, M. Nikolić, D. Ogašanović, Z. Tešović

Selection of domestic walnut (Juglans regia L.) from natural population

 

Nenad Brkić, Živorad Gajić, Milovan Pušić

The genetic and phenotypic variability and relationship of some growth and slaughter characteristics of the performance tested gilts

 

V. Bogdanović, Brkić. N.

Influence of systematic environmental factors on genetic indexes and rank of potencial sires

 

Avramov, L., Milutinović, M., Jović, S., Žunić, D., Maletić Radojka, Vujović, D.

Uvological and technological investigation of clone population in cultivar Kaberne Fran

 

Jovanka Atlagić

Cytogenetic study of the species Helianthus rigidus and its F1 hybrids with the cultivated sunflower, Helianthus annuus

 

Jan Boćanski i Zoran Petrović

Genetic variability and mode of inheritance of yield components in maize inbreed lines and its hybrids

 

Jelena Bošković, Bošković M., Pešić V.

Gene-for -gene modeling for wheat hybrids resistant to Puccinia recondita tritici

 

Mirjana Vasić, Jelica Gvozdanović Varga. Janko Červenski

Variability and heritability of plant height components in several domestic varieties of bean (Phaseolus vulgaris L.)

 

Dragana Vasić, Dragan Škorić, Gilbert Alibert

RAPD Analysis of products of fusion between cultivated sunflower Helianthus maximiliani (Schrader)

 

Sanja Vasiljević, Gordana Šurlan- Momirović, S. Katić, V. Mihailović, D. Lukić, T. Živanović

Mutual dependence among components of green forage yield in varieties and populations of red clover (Trifolium pratense L.)

 

Mirjana Vulić

Effect of systematic factors of the environment on the variability of milk production

 

Jelica Gvozdanović-Varga, Mirjana Vasić

Divergence of genotipes for dry matter yield in garlic

 

Branislava Grbović, Vasilije Isajev

Variability of Serbian spruce (Picea omorika /Panč./Purkyne) pollen germination percentage during 12-month storage

 

Guzina V., Orlović S., Kovačević B.

Poplar selection for superior growth

 

Jelena Damjanović, Maja Vračarević, Bogoljub Zečević i Slaven Prodanović

Identification of donor lines for improving fruit yield of K35 x K12 eggplant hybrid

 

Gordana Demić, Snežana Mladenović Drinić i Kosana Konstantinov

Protein marker polymorphism in set of maize inbred lines

 

Dragan J. Bukić, Dane Lukić

Alfalfa: from populations to varieties

 

Slađana Žilić, Irina Božović, V. Bekrić i Snežana Mladenović Drinić

Changes on proteins in different soybean varieties under effects of increased temperature

 

Žunić P., Bešlić Z.

Flower type in F1 generation received by hybridisation of grapevine varieties - Drenak crveni x Afuz-ali

 

Zdravković M., Čorokalo D., Đorđević R. i J. Zdravković

Effects of genetic parameters on inheriting the number of pods per plant in beans (Phaseolus vulgaris L)

 

Jasmina Zdravković, Marković Z., Zdravković M., Mirjana Mijatović

Inheritance mode of fruit shape in tomato

 

Bogoljub Zečević, Dušan Stevanović, Slaven Prodanović i Radiša Đorđević

Inheritance of yield components in diallel crossing of divergent genotypes of pepper (Capsicum annuum L.)

 

Veselinka Zečević, D. Knežević, Danica Mićanović

Variability and components of variance for plant height in different wheat cultfvars

 

Vasilije Isajev, Predrag Jović

Application of introduction as a method of tree breeding -the example of black walnut (Juglans nigra L.)

 

Vasilije Isajev, Vera Lavadinović

Variability of macroscopic characteristics of Serbian spruce (Pices omorika /Panč./ Purkyne) needles in ecologically different micropopulations

 

I. Jevtić, Z. Stanimirović, B. Soldatović

Chromosome aberrations as a cause of decreased fertility in female pigs and congenital malphormations in offspring

 

Zoran Jerković, Radivoje Jevtić

Genetic analysis of parents in wheat breeding to obligate parasites resistance

 

Jestrović Zorica, Kondić Ankica, Šešek S., Denčić, S., Pavlović, M.

Callus induction from mature embryos in species of the genus Triticum L.

 

Joksimović J., Atlagić J., Škorić. D.

Effect of genes and combining ability for kernel yield in some sunflower inbred lines

 

B. Kerečki, M. Ivanović i Violeta Anđelković

Genetic control of maize grain dry-down during maturation

 

Kraljević-Balalić Marija

Gene effects for physiological parameters in wheat

 

Bojana Klašnja, Špiro Kopitović, Saša Orlović, Zoran Galić

Variability of some structural and physical properties of black locust (Robinia pseudoacacia L.) wood

 

Desimir Knežević

Level of genetic determination of small grains and future prospect

 

Desimir Knežević, Veselinka Zečević, Milanko Pavlović, Danica Mićanović, Miroslav Kuburović, Slaven Prodanović

Genetic determination of technological quality of wheat

 

Kondić Ankica i S. Šesek

Differences between homozygous and heterozygous wheat genotypes regarding their androgenic response

 

Borislav Kobiljski, Srbislav Denčić, Marija Kraljević-Balalić i Rončević Petar

Inheritance of kernel number per spike in crossing wheat genotypes differing in Rht genes

 

Branislav Kovačević

Evaluation of discrimination ability for morphometric leaf parameters of eastern cottonwood

 

Miroslav Kuburović, Milanko Pavlović, Milivoje Milovanović, Veselinka Zečević

Characteristics of perspective lines of winter wheat of center for small grains in Kragujevac

 

Lukić D., Marija Kraljević-Balalić

Breeding alfalfa genotypes for increased seed yield

 

Milomirka Madić, Miroslav Kuburović i Aleksandar Paunović

Genetic analysis of the grain mass per plant in barley hybrids

 

Dragoljub Maksimović, Dušan Urošević, Aleksandar Paunović

Study of some characteristics of barley varieties en F1 generation

 

Ana Marjanović-Jeromela, Radovan Marinković

Heritability of rapeseed (Brassica napus L.) yield components

 

Marković Z., Zdravković Jasmina, Damjanović M.

Local populations and semi-wild tomato forms as a source of genetic divergence

 

Milan Mataruga, Vasilije Isajev, Mirjana Šijačić-Nikolić

Variability of some qualitative characters of austrian pine half-sib lines in a seed orchard on Jelovoj gori

 

Mezei S., Čačić N., Nagl N., Kovačev L. i Sklenar P.

Possible use of micropropagation of dormant buds from root head of sugar beet in recurent selection

 

Rade Miletić1, Radoljub Petrović1, Milisav Mitrović

Biologically-pomological characteristics of bushes and trees of forest European filbert (C. avelana L) and the European filbert (C. colurna L) in eastern Serbia

 

Rade Miletić

Fenological regularities in walnut trees population in region of Timočke krajine

 

Gordana Milojević, Snežana Mladenović Drinić, Goran Drinić i Kosana Konstantinov

The cluster analysis of maize inbred lines on the basis of polymorphism of proteins marker

 

Milivoje S., Milovanović, VladanPešić

Inheritance of grain number per spike in hexaploid tritikalea

 

Momčilo Milutinović, Dragan Nikolić, Milica Fotrić i Vera Rakonjac

Ratio of pollen functional ability and fruit set degree in grapevine (Vitis sp.)

 

Milutinović M.M., Milutinović D.M.

Coefficent of fruitfulness of apple varieties and hybrids

 

Milutinović. M., Žunić. P., Simić V.

Inheritance of berry skin color in seedlings of F1 generation of interspecies hybrids in grapevine

 

M. Mitrović, S. Milenković

Racemose walnut 10/95

 

Novica Mladenov, Novo Pržulj, Nikola Hristov, Veselinka Đurić

Phenotypic and genetic relationships between wheat quality

 

Jasmina Muminović, Dražen Jelovac, Gordana Radović

Variability of morphological traits of maize (Zea mays L.) inbred lines, conserved in yugoslav genebank for maize

 

Živka Bukić, Siniša Milutinović, Danica Mladenović

The possibility of using pepper populations in the aim of further breeding

 

Aleksandra Nastasić, M. Stojaković, G. Bekavac, Z. Petrović, N. Vasić

Influence from S1 recurrent selection in maize synthetic NSB

 

Biljana Nikolić,Vasilije Isajev

Group and individual pollen variability of some 5-needle pines in function of improvement

 

Dragan Nikolić

Genetic analisys of young shoot characteristics in grapevine progeny obtained by crossing Muscat Hamburg and Villard Blanc cultivars

 

Saša Orlović, Borivoj Krstić, Bojana Klašnja

Genetic and phenotypic correlation of physiological and growth parameters of poplar clones

 

Saša Orlović, Zagorka Petrov, Zoran Galić, Leopold Poljaković Pajnik

Variability of black walnut (Juglans nigra L.) physiological parameters and growth elements in the juvenile phase

 

Mirjana Ocokoljić, Vasilije Isajev, Nada Kovačević

Study of individual variability of horse chestnut (Aesculus hippocastanum L.) in the aim of improved secies selection

 

Boro P. Pavlović

The framework of variants of haploid chromosome complement producing a finite number of pure lines

 

Pavlović Nenad, Sušić Zoran, Zdravković Jasmina, Marković Živoslav, Stevanović Dušan

Variability and heritability of onion bulbs diameter

 

Sofija Pekić, Vesna Lazić-Jančić, Violeta Anđelković, M Ivanović, Zora Dajić, Evonne Waterman, H Rahman, A Steed i SA Quarrie

Putting physiology on the map - testing relationships between drought resistance traits using molecular maps

 

Sofija Pekić, Lora Ljubojević, Violeta Anđelković, M Ivanović, Vesna Lazić-Jančić, A. Steed i SA Quarrie

The role of ABA in drought resistance: physiological and genetical analysis in maize

 

Petrović, Z., J.Bocanski, Nastasić. Aleksandra, G. Bekavac, N. Vasić

Combining abilities and mode of inheritance of yield and 100-grain mass in maize (Zea mays L.)

 

Novo Pržulj, Novica Mladenov, Miroslav Bogdanović

Estimates of genetic effects of productive tillering in spring wheat

 

S. Prodanović, G. Šurlan-Momirović. D. Perović, I. Stančić, Z. Nikolić i Z. Veselinović

Structural changes of wheat populations by bulk selection I. Mean values and correlation among traits

 

S. Prodanović, G. Šurlan-Momirović, B. Jovanović i M. Menkovska

Structural changes of wheat populations by bulk selection II. Analysis of homology in variation of traits

 

Vera Rakonjac

Genetic and phenotypic correlation and path analysis in peach

 

Petar Rončević, Nikola Hristov i Marija Kraljević-Balalić

Effect of PEMF on grain number and grain mass per spike in spring wheat

 

Pavle Sklenar, Lazar Kovačev, Nikola Cačić, Snežana Mezei i Nevena Nagl

Correlations and path-coefficients analysis in sugar beets root traits

 

Mirjana Srebrić

Effects of sister-line cross on grain yield increase in soybean lines with reduced activity of trypsin inhibitor

 

Stanković G., Trifunović B.V., Trifunović V.

Accumulation of favourable alleles in two synthetic populations of maize (Zea mays L.)

 

Sikora V.

Heterosis for agronomic traits of broomcorn

 

Sušić Zoran, Zdravković Jasmina, Pavlović Nenad, Prodanović Slaven

Selecting features for estimating genetic divergence of tomato genotypes (Lycopersicum esculentum Mill.)

 

Žarko V. Tešović, Slađana A. Nidžović, Milan M. Lukić

Amino acids in the fresh fruit of the parental raspberry cultivars and promising selections (Rubus idaeus L.)

 

Žarko V. Tešović, Sladana A. Nidžović, Milan M. Lukić

Biological properties of promising apple selections

 

Žarko V. Tešović, Sladana A. Nidžović, Milan M. Lukić

Breeding dwarfing apple genotypes

 

Zorica Tomić, Sokolović, D. i Gordana Šurlan-Momirović

Inbreeding depression of productive traits in three species of the genus Agrostis L.

 

Zorica Tomić i Sokolović D.

Regeneration of perennial grasses seeds from the collection of the plant gene bank

 

Mihailo Tošić

Clonal archives of fir with fastigiate crown (Abies alba Mill. var. elegantissima Tošić) near Ivanjica - pilot plots - for protection and breeding

 

Trifunović B.V., Trifunović V., Stanković G.

Genetic potential of yield of new ZP maize (Zea mays L.) hybrid combinations

 

Trivunović Snežana, Teodorović M., Petrović Milica

Genetic parameters of the fattening and reproductive traits of swedish landrace

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

Genetic -ecological bases of Ailanthus (Ailanthus altissima Swingle) adaptation in Serbia

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

Flowering of Acer saccharinum L. occurrence, frequency of polygamy and sex mosaics

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

Neoteny types in trees and shrubs and their inportace

 

Gordana Ćurčić, Vasilije Isajev, Mihailo Tošić

Balkan Maple generative seed orchard i near Ivanjica - pilot plot for Balkan Maple breeding

 

Biljana Urošević, Jasmina Radović, Zorica Tomić

Establishing resistance to Verticillium albo atrum in the progeny of alfalfa (Medicago sativa L.) genotypes

 

Filipović M., Drinić Mladenović S., Milojević G. i Konstantinov K.

Application of protein markers in "Genetic Purity" testing of maize genome

 

Nikola Hristov, Novica Mladenov, Marija Kraljević-Balalić

Genetic diversity of stem traits in winter wheat

 

Cvikić Deian, Jasmina Zdravković, Gordana Šurlan Momirović, Sušić Zoran, Đorđević Radiša

Inheritance mode and phenotypic variability for fruit firmness in nor and rin tomato genotypes

 

Nikola Čačić, Lazar Kovačev, Snežana Mezei, Pave Sklenar i Nevena Nagl

Combining abilities, gene action, and interdependence of several traits of sugar beet root (Beta vulgaris L.)

 

Šesek S. i Ankica Kondić

Testing wheat genotypes for herbicide tolerance by in vitro culture

 

Lević Jelena i Tijana Petrović

Occurrence of the race R1 Exserohilum turcicum (pass.) Leonard & Sugss pathogenic to maize

 

Saratlić G., Rošulj M. i K. Konstantinov

Influence of long-term mass selection on changes in fatty acid composition in two high oil maize (Zea mays L.) populations

 

Nenad Delić

Estimation of genetic gain in narrow based population of maize (Zea mays L.) using different population sizes

 

 

Sekcija 5. Human Genetics

 

Koviljka Krtolica, Milica Radojković, D. Marinković, L. Ristić i Biljana Todorić

Comparison of cytogenetic analysis and polymerase chain reaction for the detection of t(14;18) in follicular lymphoma

 

O. Miljanović, M. Kaluđerović

Chromosome abnormalities in children with mental retardation associated with dyismorphia

 

Gordana Anđelić, Zvonko Magić, Ljiljana Martac, Ilija Tomić, Vladislav Stepić i Vladimir Mrđa

K-ras and p53 mutations - implications to prognosis and sensitivity to therapy in lung cancer patients

 

Vesna Verbić, Danica Grujić, Milena Stevanović

Fluorescent in situ hybridization (FISH) in clinical and fundamental investigations

 

Slobodanka Vukosavić, Serge Przedborski

Role of synuclein in neurophatology of Parkinson’s disease

 

M. Guć-Šćekić, D.Radivojević, M. Đurišić, N. Arsić, M. Stevanović, D. Zdravković, K. Sedlečki, M.Banićević

Cytogenetic and molecular studies in sexual differentiation disorders

 

Slobodanka Grković, Vesna Ivanović Deretić

Cat Eye syndrome - case report

 

N. Dedović, J. Milašin, O. Josipović, M. Vukadinović i B. Dimitrijević

Molecular genetic analysis of premalignant and malignant lesions of oral cavity

 

Jasmina Đurković

Acanthosis nigricans - Review of a case

 

Ninoslav Djelić

Mitotic and proliferation indices of human lymphocytes treated with oxytocin in vitro

 

V. Đorđević, M. Pantić, M. Đurović, A. Novak

Turner's syndrome with de novo balanced translocation t(l;9) and ring (X) chromosome

 

V. Đorđević. M. Pantić, A. Novak, N. Suvajdžić, M. Čolović

DER(14)t(l;14)(q11;q11) in chronic myelomonocytic leukemia: A new abnormality characterized by cytogenetic and fluorescence in situ studies

 

M. Djurišić, M.Guć-Šćekić, T. Lalić, D. Radivojević, D. Zamurović, M. Đurić, S. Todorović

Genotype-phenotype correlation in two DMD/BMD patients with large deletions in dystrophin gene

 

Zarovni Nataša, Georgijević Dubravka, Radojković Dragica

Allele distribution of two VNTR and two STR loci in population of Serbia

 

Zamurović N., Čuljković B., Stojković, O., Major T., Keckarević D., Savić D. i Romac S.

PCR diagnostics of Charcot Marrie tooth

 

Ilić Vesna, Cikota Bojana, Vojvodić Danilo, Malešević Milomir, Stamatović Dragana, Magić Zvonko

Mutations in the K-ras AND p53 genes in twins before and after bone marrow transplantation

 

J. Jovanović, M Guć-Šćekić, M. Kuzmanović, G. Bunjevački

Cytogenetic studies of MDS in childhood

 

A. Krstić, F. Popić - Paljić, J. Jovanović - Privrodski, M. Obrenović, R. Aleksić, M. Kolarski, I. Kavečan. T. Redžek - Mudrinić, J. Popadić, M. Nikolić, R. Madžar, Lj. Gaćina, V. Čihi, C. Laketa, P. Kruščić, J. Rudež, T. Tarašenko, M. Grubješić

Prenatal diagnosis and health promotion

 

Aleksandar Krstić, Đorđe Mačvanin, Feodora Popić, Jadranka Jovanović, Ružica Aleksić, Milan Obrenović, Ivana Kavečan, Tatjana Redžek-Mudrinić, Jelena Popadić, Marko Nikolić, Milka Mitrović, Spomenka Mijić

Langdon down syndrome in Vojvodina - Epidemiology, Dermatoglyphics and New Treatments

 

Kečkarević P., Čuljković B., Stojković O., Vukosavić S., Savić D., Zamurović N., Major, T., Romac S.

Spinal muscular atrophy- Possibilities for prenatal diagnostics

 

T. Kuveljić, J. Nikoliš, Novaković, V. Bunjevački, Lj. Luković, J. Milašin, M. Krajinović, S. Radmanović, S. Branković

The role of chromosomal polymorphism in sex chromosomes meiotic nondisjunction

 

D. Luković, I. Novaković, J. Milašin, Lj. Luković, T. Kuveljić, V. Bunjevački, M. Krajinović i N. Ostojić

Loss of heterozygosity on chromosomes 3p and 6p in cervical carcinomas

 

Zvonko Magić, Bojana Cikota, Vesna Ilić, Gordana Anđelić

P53 mutations in breast cancer patients

 

Major T., Culiković B., Stojković O., Zamurović N., Kečkarević D., Savić D. i Romac S.

Fragile X syndrome: Molecular diagnostics

 

Danko Obradović, Aleksandar Krstić, Jasmina Rudež, Milan Obrenović, Ivana Kavecan, Jelena Popadić

Sex determination of human fetus by amplification of DNA molecules from amniocytes

 

M. Pantić, A. Novak, V. Đorđević, D. Marisavljević, N. Suvajdžić, G. Janković, *M. Stevanović, M. Čolović

Cytogenetics and fluorescence in situ hibridization in diagnosis of myelodisplastic syndromes

 

Sonja Pavlović, Milan Joksimović, Milica Cvorkov-Dražić, Gordana Bunjevački, Dragana Janić i Zvezdana Popović

Molecular diagnosis of inherited hemoglobinopathy – Hb lepore

 

Branka Popović, Jelena Milašin, Miroslava Mičić, Vitomir Konstatinović

LOH in the regions 3p21,9p21,6q21-23,17p13 AND 13q14 in squamous cell carcinomas of the lip

 

D. Radivojević, M. Guć-Šćekić, M. Đurišić, T. Lalić, J. Savić, P. Minić, A. Cvetković, E. Kanavakis, M.Tzetis, T. Antoniadi

Genotype/phenotype correlation (as to the pancreatic status) of Yugoslavian cf patients

 

Rakićević Ljiljana, Georgijević Dubravka, Janković Gordana, Miković Danijela, Radojković Dragica

Prenatal diagnostics of hemophilia a by intragenic RFLP analysis

 

Savić P., Čuljković B., Stojković O., Major T., Zamurović N., Kečkarević D. i Romac S.

Molecular genetics and diagnosis of myotonic dystrophy

 

Biljana Spremo-Potparević, Vesna Verbić, Milena Stevanović i Milan Joksimović

Determination of the time of human chromosomes centromere segregation with effect of PCD by fluorescence in situ hybridization

 

Biljana Todorić, Z. Magić, L. Ristić. M. Malešević i K. Krtolica

Detection of Philadelphia chromosome negative chronic myeloid leukemia by RT-PCR method

 

Geza Cekuš

Dyschromatopsia rate among the population of northern Backa (Voivodina)

 

Geza Cekuš

The ability of perceiving the smell of acetone

 

Bojana Cikota, Rajna Stamenov, Slavko Berger, Danilo Vojvodić, Milomir Malešević, Zvonko Magić

K-ras AND H-ras point mutations in patients with large cell lymphomas and chronic lymphadenitis

 

Čuljković B., Stojković, P., Vukosavić. S. i Romac S.

Human identification and patternity testing

 

Jan Kišgeci, Janoš Berenji

Transgenic plants - pro et contra



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETICS AT THIRD MILENIUM BEGINNING

 

Kosana Konstantinov

 

Maize Research Institute "Zemun Polje", 11081 Zemun-Belgrade, Yugoslavia

 

Second Congress of Serbian Geneticists is going on at the end of 20th Century, culminating the tremendous development of our science in this century. Historically, First International Conference on this subject was held during January 1899 in London, England and Second during September 1902 in New York City. Both meetings have been named as "International Conference on Plant hybridisation". During Third Conference, held in London during 1906. Title was slightly modified to "International Conference on Hybridisation and Plant Breeding". During this meeting the term "Genetics" has been suggested for this new field of scientific inquiry. After development of all fields of science, including genetics, a serious question has been raised whether human species can sustain itself on earth. The answer is affirmative. Mankind not only can survive but also aspire to live at high quality. In order to achieve this goal, co-operation among scientific workers in all fields have to be established and geneticists can play a significant role in three basic fields:

1. Agriculture based on gene structure and function understanding;

2. Medicine based on gene structure and function understanding and

3. Environmental protection based on gene structure and function understanding.

If estimation for human population for the first half of 21.century' are about 8.9 billion the first consequence will be increase in the demand for food. By shortage in water supply desert encroachment and diminution of arable acreage could be expected. Several fundamental tasks geneticists must be involved: establishment of germplasm banks. Release of captured genetic potential and Development of new breeding technologies. In the field of human genetics, through co-operation among geneticists, physicians and pharmacologists the main contribution the understanding and treatment of single- and multiple-gene based diseases: understanding of gene role in cancer development, ageing and life expectance and introduction of routine application of gene therapy in disease treatment, particularly in the nervous system development and function.

As we have only one earth to live on, existing research should be intensified in the field of genetics covering problem of the soil, atmosphere and water contamination. With the rapid development of industry' and public transportation, damages to the environment have caused direct and serious threat to human health. Among many of important tasks in the field of the environmental protection, selection of already existing fungal strains, as well as through mutation induction of new strains, which can digest and utilise pollutants for their own use is promising. Also the selection of microorganisms for biological degradation of pollutants in water and soil. Particular task is development of highly sensitive genetic methods for testing the presence and estimating the amount of mutagens/carcinogenes in the environment.

In the coming century it could be predicted that only through the study of the genome the life phenomena themselves can be understood.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MOLECULAR GENETICS OF LACTIC ACID BACTERIA IS A BASIS FOR DEVELOPMENT OF MODERN DAIRY BIOTECHNOLOGY

 

Lj. Topisirović. M. Kojić, Dj. Fira, N. Miladinov, I. Strahinić, O. Gajić, S. Arsenijević i I. Spasojević

 

Institute of Molecular Genetics and Genetic Engineering, V. Stepe 444a, P.O.Box 794,

11001 Belgrade, Yugoslavia

 

Lactic acid bacteria (LAB) have been essential in food and feed fermentation for centuries. They have very broad application in food industry and agriculture. They have also a great influence on nutritional attributes of the products such as flavour, texture and others. In addition, some of them are commensal colonisers of the mammalian gastro-intestinal tract including humans, where Lactobacillus acidophilus and Lactobacillus renteri are commonly found. Some strains of LAB are probiotics, since it is believed that they have effect on human and animal health. Health benefits attributed to LAB are following: (a) control and treatment of infections in the intestine caused by enteric pathogens or infections in the urogenital tract; (b) reduction of incidence of tumour/cancer in colon or reduction of the level of serum cholesterol as well as cardiac heart disease and (c) stimulation of immune system and bowel movement. Bearing in mind these probiotic effects of LAB, an increasing interest exists for dairy products containing specific bacterial species with potential health-improving properties. These products are named "functional food".

Studying the genetic potentials of LAB isolated from the traditionally homemade fermented products produced in different geographical regions is extremely interesting. A collection of natural isolates (about 600 isolates) was made. This collection contains LAB isolated from different traditionally prepared fermented products throughout Yugoslavia. Numerous isolates were tested for the production of proteinases, exopolysaccharides, and bacteriocins. Most of those showing interesting characteristics from scientific and commercial point of view were analysed at molecular level. Among tested LAB, isolates were characterised that produces new type of proteinases or bacteriocins. In addition, such LAB could be a source of exopolysaccharides or aromogenic compounds having high commercial value. Dairy products acquire their unique characteristics from differences in the composition of starter cultures. However, genes encoding production of these components are very often located on the genetically less stabile elements, plasmids. Therefore, studying the molecular genetics of LAB especially by using genetic engineering will facilitate the construction of more stabile starter cultures. Starter cultures could be constructed by combination of genetically characterised natural isolates. On the other hand, selection of natural isolates with probiotic capacities opens a possibility to construct the starter cultures for functional food production. Taking altogether, construction of improved, reliable starter culture is a main task for molecular biotechnologists in the future.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MOLECULAR BIOLOGY AND INFORMATION TECHNOLOGY IN PLANT BREEDING

 

Drinić Mladenović Snežana, Jelovac Dražen i Konstantinov Kosana

 

Maize Research Institute "Zemun Polje", 11081 Zemun-Belgrade, Yugoslavia

 

In the past two decade, development of molecular genetics including molecular technology for gene transfer from heterelogous organisms into plants brought a new dimension of plant breeding. Molecular markers as universally methods for all biological systems virtually effect on success of directly examination of structure and function as well as determination of genetic polymorphism of plant genomes. The potential applications of molecular markers in plant breeding are: analysis of molecular basis of evolution; germ plasm identification, classification and management; assessing genetic diversity; identification of genes underlying agronomy important traits as yield, resistance to stress and disease and heterosis as well. High density genetic linkage maps for a number of plant species as a basis for marker assisted selection of agronomically useful traits and isolation of these genes have been established. New class of molecular markers known as "gene chips", uses microarrays of molecules immobilized on solid surfaces have recently been developed. With "gene chips" DNA belonging to thousand of genes can be arranged in small matrices (chips) and hybridized with labeled cDNA. The first biological application of DNA chip technology are analysis of gene expression and it should help to better understanding of fundamental mechanisms of plant growth and development, polymorphism detection and rapid analysis of transgenic plants.

Great move in the field of plant biotechnology are possibility to introduce genes from heterelogous systems into plant genomes resulting in plants with altered traits or in another word transgenic plants. The experiment of maize genome transformation by introducing gene coding neomycin phosphotransferaze (NPT II), chloramphenikol 0-acetiltranspheraze (CAT) and part of A. tumefeciens bacterial genome have been started during 1986 at Maize Research Institute. Both gene expression and integration of introduced genes in maize genome was determined. Integration of forcing genes induced different phenotypic changes. Changed genotypes were chosen as model system for further investigations and genes identification controlling concerned traits. Transgenic plants with genes for resistance to insects and pests, tolerance to herbicides or improvement quality are already available on market. First generation of biotechnology experiments enable transfer of single gene but by now days experiments transfer of few genes together and using plants as bioreactors are possible.

Important factor in the success of the genetic improvement of crops was development of faster and more reliable methods, which allowed easier analysis of date as well as rapid information exchange. The application of information technology and development of statistical techniques to analyze genomic information is know as "bioinformatics". By integrating genetics with informatics investigations of whole genomes aims to elucidate the structure, function and evolution of plant genomes are faster. Together these technologies as integral part of classical breeding programs contributing significantly to shorting of plant breeding process.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MODULATION OF GENE EXPRESSION BYANTISENSE OLIGONUCLEOTIDES

 

Selma Kanazir, Sabera Ruždijić, Ljubisav Rakić

 

Institut za biloška istraživanja "S.Stanković", 29. Novembar 142. Beograd

 

Antisense-oligonucleotides (ASON) are synthetic, single stranded DNA or RNA segments, 15-25 nucleotides in length. Their sequence is designed to be identical to antisense DNA strand and to block the expression of specific gene by binding to its DNA or RNA in a sequence-specific manner. They exist in several forms. Antigene, oligonucleotides • mat bind to DNA and inhibit transcription through formation of triplex DNA. Ribozymes, catalytic antisens RNA molecules. Antisense oligonucleotides that bind to iRNA and block translation. Aptamers. oligonucleotides that specifically interact with proteins. Selected ASON is unique and specific in its capability to inhibit the expression of single targeted gene at any level of its regulation from DNA via RNA to protein. Their ability to modulate gene expression opened a new genetic strategy for the study of physiological and pathophysiological role of a gene by loss of its function. Moreover, the completely new field in developing gene specific therapeutics, antisense therapeutics, has emerged. Since, virtually all diseases are associated with abnormal or inappropriate gene activity, antisense oligonucleotides represent a powerful therapeutic tool especially in cancer and viral infections.

The application of antisense strategy for tlie inhibition of CD44 gene expression will be discussed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POPULATIONAL AND EVOLUTIONARY ASPECTS OF B-HROMOSOMES -

A case of yellow-necked mice

 

Mladen Vujošević i Jelena Blagojević

 

Department of Genetics, Institute for Biological research "S. Stankovic"

29 novembra 142, 11060 Belgrade, Yugoslavia, e-mail: mladenvii@ibiss.bg.ac.yu

 

B-chromosomes are supernumerary to the normal chromosomal complement. They differ from the main complement by their unequal distribution in species and irregular behavior during meiosis. According to definition B-chromosomes are not essential for the survival of their carriers. They persist in spite of that and are present in more than two thousand plant and animal species. In spite of numerous investigations a general agreement on the origin, role and mode of persistence of Bs in populations' has not been reached. A number of theories exist of which the parasitic model dominates over others. This model assumes the existence of mechanism that lead to the accumulation of B-chromosomes in gametes and the establishment of a balance between the degree of transmission of B-chromosomes and the elimination of their carriers due to lowered fitness. Opposed to this model is the heterotic model which assumes the existence of adaptive advantages of B-chromosome carriers. In a large number of investigated cases the parasitic model was used to explain the mechanism mat maintain Bs in populations, whereas the heterotic model was supported by a few cases only. In populations of the yellow-necked mouse, Apodemus flavicollis, B-chromosomes are present in different frequencies that correlate with the altitude and climatological variables. Some populations remain in equilibrium for years with regard to the frequency of specimens with B-chromosomes. Nevertheless, they display seasonal variations. The mechanism of the maintenance of Bs in populations does not fit any of existing models. The effects of Bs are not noticeable at the phenotypic level. Morphometric and analysis of developmental homeostasis has uncovered the consequences of their presence in the genome. In support of this is the discovery of DNA sequences that are only found in sample of species with B-chromosomes.

Recent investigations at the molecular level suggest that B-chromosomes are very useful for studying genome evolution. Therefore ii is of great importance that these investigations are included in complex populational-evolutinary investigations.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POPULATION GENETIC ASPECT OF STRESS

 

Marko Anđelković i Marina Stamenković-Radak

 

Faculty of Biology, University of Belgrade

 

The interaction between individuals of a population and their environment is of great importance for species survival and conservation. If organisms are unable to either adapt to slow environmental changes, or react to rapid ones, such as climate change, pollution, ecological catastrophies of various degree, populations, and even the species, may become extinct. The evolutive strategies of species and genetical changes at the population level differ according to the environmental effect and genetical structure of populations.

Under stress conditions, the genotypes that allow rapid establishing of individual homeostasis of the cell or organism are favored. Although selection represents the slowest mode of the response to stress, it ensures a long term adaptation of the population to stress. Stress can be environmental, caused by external factors, and genomic, caused by another genome. Many population-genetic parameters are affected by stress.

Different approaches to studying the effect of environmental changes on the development homeostasis and genetic variability of the populations, as a response to stress, are illustrated by the examples on Drosophila species. One approach is based on the variability of the trait with a known genetic basis (Amy genotypes, inversion karyotypes). It attempts to correlate a given variability with fitness in modified laboratory environments and various natural habitats.

Another approach is based on the analysis of phenotypic variance, appearing in the response to stress, which determines the type of variability. By applying selection genotypes with extreme phenolypic values can be isolated. The organisms respond to environmental stress at the level of phenotypes by behavioral, physiological, or morphological adaptations, which can appear in the form of stress avoidance or build up of resistance. In addition to the variability associated with response to stress, genetic variability for the plasticity of response can be distinguished too. It is disputable whether the extent of stress is related to the changes in the population at the level of gene pool, or via changes in plasticity'.

Prediction of the ability of a species to evolve in the response to fast environmental changes is a major task of evolutionary biology. The populations of genus Drosophila can be suitable indicators of environmental changes. Factors limiting species distributions and genetic characteristics of populations (marginal and central) represent high priority for future research.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PLANT ANTIMUTAGENS AND CANCER PREVENTION

 

Draga Simić, Jelena Knežević-Vukčević


Laboratory for Microbiology, Faculty of Biology, University of Belgrade

 

The possibility to modulate the response of a cell to environmental and endogenous mutagens has opened a new frontier for cancer control. The concept of preventing cancer by delay, i.e. by decreasing the rate of mutation accumulation, promoted the study of plant antimutagens as possible dietary anticancerogens.

According to their mechanisms of action antimutagens are placed in two major categories: desmutagens, which prevent the DNA damage and bioantimutagens. which modulate DNA repair and replication. In recent years a number of plant desmutagens (tannins and other phenolics, mono and diterpenoids, flavonoids, vitamins, etc.) were identified and their mechanisms of action include: inhibition of endogenous formation of mutagen, inhibition of activation of promutagens, antioxidant activity and scavenging of free radicals, blocking of mutagen or competition with, induction of detoxifying enzymes or acceleration of mutagen decomposition. Moreover, many plant desmutagens were found to inhibit tumor promotion and progression. On the oilier hand, only a few plant bioantimutagens are known (cinnamaldehyde, coumann, vanillin, umbelliferone, tannic acid) and they prevent mutations by stimulating the error-free DNA repair. In our laboratory antimutagenic potential of terpenoid fractions of sage (Salvia officinalis L), as well as pure monoterpenoids, has been investigated Prokaryotic and eukaryotic tests applied measure different genetic end-points: reverse mutations, replication fidelity, recombination, induction of mutagenic repair, chromosome aberrations. Detection of bioantimutagens was performed with the new E. coli K.12 assay (Simić et al, 1997). Antimutagenic potential of antioxidants from sage was "creened with E. coli and S. typhimurium tests (Mitić et al.. 1998). The results indicate that multiple mechanisms are involved in mutagenesis inhibition by terpenoids from sage. The protective effect of sage extracts against UV-induced mutations was confirmed in S. cerevisiae D7. Preliminary experiments indicated that dietary exposure to terpenoids from sage suppressed in vivo mytomicine C induced chromosome aberration in mice. Further study will investigate the possibilities of using sage terpenoids as anlicarcinogens.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE USE OF TRANSGENIC ANIMALS IN CARCINOGENICITY TESTING

 

Roy Forster

 

Centre International de Toxicology, 27005 Evreux, France

 

There is much current interest in the use of transgenic approaches in the evaluation of chemical carcinogenicity. This is already reflected in international guidelines for the safety testing of new drugs (from ICH), according to which the results of transgenic assays may be presented in the evaluation of carcinogenicity. An international ring-trial co­ordinated by 1LS1 (International Life Sciences Institute) is underway for the evaluation of four promising transgenic mouse strains. These are (i) the heterozygous p53+/- knockout mouse which lacks one copy of the p53 gene and shows enhanced sensitivity to genotoxic carcinogens, (ii) the TG.AC strain which bears a transgene containing the rets oncogene and has been found to show an enhanced sensitivity to the induction of dermal papillomas, (iii) the rasH2 strain which carries a transgene containing the human c-ras gene, complete with regulatory sequences of DNA and (iv) the xpa mouse in which the xpa gene is interrupted and excision repair of DNA is not functional. If the evaluative ring-trial produces encouraging results for the reliability and sensitivity of these transgenic bioassays, then they offer attractive advantages. The end-point is tumour production in a whole animal, underlining the relevance of these assays. Furthermore, evaluation of the tumour response takes place when the animals are still relatively young, avoiding the confounding effects of ageing. At present, the testing strategies to be deployed with these strains are not well defined. This presentation will examine the genetic basis of the assay systems, which is relevant to strategies of utilisation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETICS AND BREEDING OF MAIZE

- ACHIEVEMENTS AND CHALLENGES -

 

Drinić Goran i Babić Milosav

 

Maize Research Institute, Zemun Polje, Zemun -Belgrade

 

Genetic improvement of plant and animal species endured through millennia, firstly as domestication and then as continuous selection for phenotypes desired by breeders and farmers. The base for scientific breeding programmes was established by discoveries referring to a sex and inheritance in plants and by a re-discovery of Mendelian inheritance principles. Breeding programmes were aimed at the achievement of economic goals. The improvement and advancement in conventional breeding are related to the progress within the following segments:

- Improvement of breeding methods

- Maintenance and broadening of genetic variability

- Development of models of quantitative genetics

Plant breeding is a slow and painstaking process, requiring not only time (backcrosses and numerous tests), but also the aim achievement is sometimes uncertain (incorporation of genes from wild relatives).

Numerous breeding strategies are characterised by common elements such as necessity to obtain the flow of new and diverse germplasm, develop new gene combinations and identification of the best ones to be used in deriving new, improved varieties and hybrids with the same principal goal - the increase of yielding potential, tolerance to economically important diseases and pests, then improvement of quality and tolerance to stress environmental conditions.

Since a genetic base for the majority of economically significant traits is not completely clarified, superior individuals, selected for the development of the succeeding generation (cycle), will be identified by testing the progeny or will be based on individual performances. The empirical results clearly confirmed such an approach. The genetic potential of maize grain yield has been annually increasing by about 100 kg ha-1 during the last 80 years. The similar improvement was achieved in some other economically important plant species (rice, wheat, soybean, cotton, etc.). Although extremely successful in maintenance and improvement of agricultural species, such approach to plant breeding did not provide the complete comprehension of genetic control and manipulation (regulation) of complex genetic phenomena.

Biotechnological studies have been very intensive during the last two decades. However, molecular biologists are chiefly able to study (analyse) simultaneously only several genes, while economically the most important traits (so called quantitative traits) of plant species are controlled by combined action of several genes. Hence, the contribution of molecular genetics to our comprehension of genetic control and phenotypic expression of these traits is far more insignificant than it was initially expected.

In the last decade of the 20th century, the determination of a structure and expression of the genome brought about a mini-revolution within research on the genome. It seems that the combination and imbuement of molecular and quantitative genetics enlighten mechanisms of genetic control, regulating inheritance of quantitative traits, and therefore it is possible to accelerate and improve applied biological research. Furthermore, it became clear that the genomic revolution could not replace activities of a breeder, but more profound comprehension of complex genetic phenomena (Heterosis, epistasis, genotype x environment interaction...) would enable more efficient identification of parents within breeding programmes and development of more efficient breeding strategies. Broadening of our perspective on the field of quantitative and molecular genetic theory is one of the essential prerequisites, and therefore, scientific experts should be qualified within a field of intersection of molecular and quantitative genetics.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ACHIEVEMENTS OF SUNFLOWER BREEDING AT THE END OF THE 20th CENTURY

 

Dragan Škorić

 

Institute of Field and Vegetable Crops, Novi Sad

 

Until 1970, sunflower breeding was characterized by the development of Russian high-oil varietal populations. The discoveries of CMS sources by Leclercq (1969) and Rf genes by several authors marked the beginning of a new era in sunflower breeding - an era characterized by the utilization of heterosis and development of highly productive sunflower hybrids. A large number of productive hybrids have since been developed in our country and a number of breeding centers around the world. This has contributed to a sharp rise in total sunflower acreage worldwide and has also helped increase and stabilize yields at a higher level than before. Using wild species through interspesific hybridization, researchers have found sources of genes for resistance to the following pathogens: Plasmopara helianthi, Puccinia helianthi, Verticillium albo-atrum, Verticilllum dahliae and Orobanche cumana. Yugoslav breeders have made a significant contribution to tliis with their discovery of sources of field resistance to Phomopsis. Also, an enviable level of tolerance has been achieved in the case of several other pathogens (Sclerotinia sclerotionmi, Macrophomina phaseoli, Alternaria helianthi). Sources of high tolerance to stress (drought) have been found as well. As of 1990, new races have been appearing in a number of pathogens. In an attempt to find sources of resistance to these new races, researchers have begun using wild species. The development of hybrids for special purposes has also yielded significant results in the last ten years, during which a large number of high-oleic and confectionery hybrids have been developed. In the past seven to eight years, the major trend in sunflower breeding worldwide has been the introduction of new biotechnology methods (anther cultures, protoplast fusion, molecular markers). Sunflower scientists have also begun working on the incorporation of genes from other species into the cultivated sunflower by transgenic methods.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFICIENCY OF S1, HS AND FS METHODS OF RECURRENT SELECTION IN SYNTHETIC POPULATIONS OF NARROW AND BROAD GENETIC BASE OF MAIZE (Zea mays L.)

 

M. Ivanović, Violeta Anđelković

 

Maize Research Institute, Zemun Polje, S. Bajica 1, 11080 Belgrade-Zemun

 

Two maize synthetic populations of narrow and broad genetic base, (B73xMo17)F2 and ZPSin 4, respectively, were used in the present study. F2 generation of the population of narrow genetic base was used as an initial cycle (progeny of two homozygous inbred lines), while the population of broad genetic base was developed by the cross of 120 inbred lines of different origin after four recombinations under random mating conditions.

Three methods of recurrent selection were simultaneously applied to both populations: S, (progenies of the first selfing), FS (full sib progenies) and HS (progenies with an unrelated inbred line F7R - used as a tester for both populations). Each population and each method of selection considered 100 randomly selected progenies planted according to the Nested design with the selection intensity of 10% cycle"1. Selection for grain yield was conducted and in such a way four cycles of selection for each synthetic, i.e. each method of selection was completed.

In order to compare efficiency of presented methods of selection in synthetics of narrow and broad genetic base, results on grain yield of all cycles of selection of synthetics per se, as well as, of progenies of certain cycles, i.e. methods of selection with two unrelated testers F7R (inbred used as a tester in the process of selection) and ZPL39. were used. Field plot experiments were set up according to the RGB design in 1995 (three locations) and 1996 (two locations), separately for the synthetics per se and HS progenies.

Results were processed by the method of the analysis of variance of the RGB design, while selection progress was expressed by the estimates of linear regression coefficients for the appropriate cycles of selection. Obtained results point out that selection progress depended on the synthetic population, as well as on the applied method of selection. The highest estimate of linear regression coefficient for the improvement of the population per se (b=0.609 t ha-1 cycle-1) was obtained for the synthetic of broad genetic base with the application of the HS method of selection. The same method of selection in progenies of the same population with the tester F7R resulted in the highest regression coefficient (b=0.9591 ha-1 cycle-1).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENE POOL CONSERVATION AND TREE BREEDING

 

V.Isajev, A.Tucović


Faculty of Forestry, Belgrade

 

Previous study of population characteristics and the effects of natural and artificial selection on the genetic structure of tree populations contribute to the conservation and enhancement of autochthonous species gene pool. The balance model of genetic structure of tree populations reveals their new properties and requires further research, especially of the relations of subpopulations, half-sib families and organisms to variable environmental factors. The interaction of these two systems is of fundamental significance for the exchange of genetic material within natural and cultural communities of trees and shrubs.

The conservation of genetic resources of populations is a part of the complex strategy of tree breeding and it is realized through different forms of conservation: in situ -natural reserves, national parks, seed stands, tree groups and individual trees, and ex situ -arboreta, live archives, seed orchards, provenance tests and progeny tests. Each strategy has its own advantages and disadvantages, both for breeding and for the conservation of biodiversity and genetic resources that makes it possible. The advantages and disadvantages are the result of the classical methods of tree and shrub improvement. They are restricted because: (1) useful properties can be improved only between the related plants, (2) the selection of the best possible combinations of characteristics lasts for a very long time and (3) in crossing, the entire genomes are crossed and not individual genes of the selected trees.

The relationship between gene pool conservation, tree improvement and rational utilization of forests is accomplished through: the balance between in situ conservation of forest genetic sources and sustainable forest management on the one hand and management of protected lands on the other hand. The conservation and management of genetic resources in situ and ex situ and the breeding activities are complementary strategies.

The focus of activities in tree gene pool conservation should be shifted from the physical protection of stands or trees to the conservation of their reproductive function and thus the potential genetic variability should be directed into free genetic variability, available to natural and directed selection.

The increased utilization of the genetic potential in tree breeding should be realized through the following activities: improved production of selected seeds of tree species, which implies the knowledge of genetic structure and variability of seed stands; establishment and management of seed orchards as specialized plantations for multiannual production of genetically good-quality seed of significant tree species, as well as through the application of the concept of new selections, by the models adapted to the requirements of forestry, which include the genetic characteristics of the species, the components of tree yield and the effect of the environment.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

NEW APPROACH IN TOMATO BREEDING (Lycopersicon esculentum Mill.)

 

Živoslav Marković, Jasmina Zdravković, Mirjana Mijatović

 

Agricultural Research Institute SERBIA, Centre for Vegetable Crops, Karadordeva 71, 11420 Smed. Palanka

 

The first tomato variety was breeded before 130 years and now has more man 2000. Foremost was breeded varieties, and after 1930. year F1 hybrids. Now varieties use only in late and processing production. As the genetic variability of tomato is narrow (from the reason of genetic erosion - electropheretic studies of alloenzymes show great similarities between the »European« and primitive cultivars) for amelioration of quality and diseases resistance of modern cultivars breeders use the »exotic« germlasm of wild and sami-wild relatives. Breeder teams make thats in state and private institutions of USA, Canada and France. Others benefit »elite« germplasm of new breeded and improved cultivars, to speed breeding process (many of properies, especially for disease resistance, are controlled by dominant genes). Also, breeders work on amelioration of photoperiodic activity, resistance to drought and high temperature, good emergence on low temperature, metabolism modification by change of concentration of CO2 and oxygen. Great possibilities provide application in breeding of biotechnology (somoclonal variations, fusion of protoplast, gene isolation, cloning and anther culture, use of genetic markers) and genetic engineering. Both are the challenge of new generation of tomato breeders. The first results are gained (tolerance to AMV and TMV incorporated of viral gene from oat. resistance of tomato to total herbicide Roundap by introduction of mutant for EPSP synthesis, resistance to Lepidothere and etc.).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CONTRIBUTION OF GENETICS IN BREEDING OF SMALL GRAINS

 

Desimir Knežević1, Novo Pržulj2

 

1ARI SERBIA, Center for Small Grains, Kragujevac

2Research Institute of Field and Vegetable Crops, Novi Sad

 

The plant breeding is older than sedentary agriculture is based on the quite well production of non-shattering plants which have been available to these early farmers. The plant breeding began by early farmers to select crop genotypes for sedentary agriculture 10-12 thousand years ago was still in use with few refinements until 100 years when made major discoveries about sex and heredity plants (Wessman De Vries etc). However, in 1901 Menders Low of Heredity have got most importance in plant breeding, as well in the same time for breeding of grains.

The creation of new desirable genotypes began 100 years ago. Since than, plant breeding has been honed and refined in next area activity l)development of techniques and procedures; 2) collection of natural genetic variability and its use-in the present , future evaluation and plant breeding programs: 3) some techniques, such as tissue culture, colchicines treatment, induced mutation which have expanded the breadth of germplasm that the plant breeder could use. The genetics, biochemists and cell biologists in last four decades have been using sophisticated instruments and model organism to learn about the genetic material DNA, and how it functions to expression of phenotypes.

Plant breeding directs towards economic objectives. Genetics is basic to rational plant breeding but the biotechnology involves other sciences such as chemistry, plant pathology and biometry.

Traditional plant breeding involves making numerous crosses from parents, which have been selected for desirable attributes and on visual criteria and measurement of many traits. In some of derived progenies were combined the best traits from the parents. Through numerous cycles of selection, a new cultivar can be developed which carry combination of the best desirable traits from parents. The conventional plant breeding programs are long-term work and effort of breeders who are looking for optimal selection procedures. The complexity of the breeders work lies in the fact that up to 10 or more years may be necessary between the initial cross and the approval of new cultivar. The breeders permanently looking for answer on the few questions example: What resources are required for genetic progress of cereals? Which methods are most efficient in cereals breeding? How to broaden genetic variability? There is competition between breeding companies to reduce time of necessity time of cultivar creation. There are also various limitations to plant improvement through conventional breeding. Besides long term of expectation of new cultivar as a limitation factor in conventional plant breeding limitation factors are also expenses for necessity analyses of large number of plants and many requirements for plant cultivation. But in some cases also, the breeder has limited genetic variation available for its incorporation into new cultivars. The various techniques developed in molecular biology and biotechnology can be used in plant breeding. The technical advanced in recombinant DNA and molecular biology methods have been developed and should have now a major impact on the efficiency and success of conventional breeding. Those methods are contribute to increasing of our knowledge of the inheritance and are providing manipulation of responsible genes which controlled certain biological traits.

In relation to the social demand, the aims of breeding programs are stilt to increase grain yield potential, to increase resistance to diseases and pests and to improve quality and tolerance to environmental stresses.

Crop improvement is slow and tedious process involving operations which not only consume great amount of time, such as backcrossing and testing, but some of which are just very difficult to achieve, for example incorporation of wild genes or inbreeding naturally crops. The numerous of molecular and molecular genetics technologies have a great impact for direction of breeding strategy, at various specific steps assisting the breeder in making achievement to a more manageable level.

There are different breeding strategies which characterized common elements: 1) means of achieving flow of new and diverse germplasm into the program; 2) new gene combinations; 3) identification of best combinations; 4) exploitation for cultivar and hybrids development. Some biotechnical methodologies can be applied at several of those steps: a) somatic hybridization to generate new hybrids; b) direct nuclear substitutions into alien cytoplasm; c) mutation/selection and cell genetics; d) generation of somaclonal variation; e) genotype fingerprinting; 0 germplasm preservation; g) gamete breeding schemes; h) gene isolation, transfer and expression: i)molecular site directed mutagenesis in situ.

Highly specific genetic selection methods would require diagnostic biochemistry techniques involving monoclonal antibodies, molecular probes and sophisticated methods of isozyme, proteins and sequence DNA analysis. The breeders in the future need to become familiar with these methods. The development of biotechnological tools can assist the improvement of plant breeding as well as seed production and genotype protection. Extensive investigation are conducted on: a) a description of molecular markers tagging the genetic control of agronomic traits and their applications for marker assisted selection: b) the identification of the physical structure and DNA composition of chromosomes and their similarity among species and; c) gene transformation methods.

The genetic maps of crop species play important role for cultivar improvements because plant breeders are able to directly manipulate specific genes by exploiting "marker assisting selection". A molecular marker represents a way to hasten the transfer of desirable traits by earning out a selection not directly on the trait, but on the molecular markers tightly linked to it.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE EXPANSION OF TRINUCLEOTIDE REPEATS AS A GENETICAL

CAUSE OF NEUROLOGICAL AND PSYCHIATRIC DISEASE

 

Romac S.

Faculty of Biology

 

The expansion of trinucleotide repeats is the cause of 12, so far, detected hereditary neurological diseases. This type of mutations was discovered few years ago which is the first and only type of mutations found exclusively in humans. These mutations are not inherited by Mendel low and the diseases, which they cause, show the phenomenon of anticipation. Anticipation is the phenomenon describing the fact that the disease, in next generation, has an earlier age of onset and more severe clinical manifestation. Genetical basis for anticipation is the expansion of trinucleotide repeats from generation to generation. If there are more triplets in mutated gene the clinical manifestation is more severe and the disease has an earlier onset. So far all the diseases of trinucleotide repeats were/are connected to hereditary neurological diseases (Huntington chorea, Spinocerebellar ataxia type 1-7, Dentatorubral Palidoluysian atrophy, Myotonic dystrophy etc.) Many of psychiatric diseases like schizophrenia, autism, bipollar disorders, also show the phenomenon of anticipation and non-Mendelian type of inheritance, are candidates for the type of trinucleotide repeats diseases.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

APPLICATION OF MOLECULAR TECHNIQUES IN DIAGNOSTICS OF CHROMOSOMAL DISORDERS

 

Milena Stevanović1 i Marija Guc-Šćekić2

 

1 Institute of Molecular Genetics and Genetic Engineering, Belgrade
2
Mother and Child Health Care Institute, New Belgrade

 

Chromosomal disorders are consequence of structural and numerical chromosome aberrations. Approximately 20% of all conceptions (embryos) have chromosomal aberration, but most of them are spontaneously aborted and frequency of chromosomes aberrations at birth is 0.6%. Not all chromosomal rearrangements are associated with phenotypic expression. It has been generally accepted that autosomal aberrations result in delay in psyhornotoral development, while sex chromosome aberrations are associated with irregular sexual differentiation and sex determination. Therefore, precise and reliable prenatal and postnatal diagnostics of chromosomal aberrations is of great importance for planning and obtaining health individuals.

In seventies, cytogenetic was revolutionized by introducing chromosome banding techniques, such as G, Q and R banding. Banding techniques, for the first time, allowed accurate differentiation of chromosomes. Chromosome banding permits more accurate diagnostics of chromosome aberrations, but this technique also have some limitations including inability to detect microchromosome rearrangements and rare mosaic karyotypes and to identify marker chromosomes. In the last few years, the fast progress has been made in more accurate diagnostics of chromosome aberration by application of new molecular techniques. FISH (Fluorescent in situ hybridization) is new highly sensitive technique, that enables visual identification of chromosomes or part of chromosomes fixed on slides. FISH has been used, as an addition to routine cytogenetic analysis, to achieve a higher sensitivity and specificity. Application of new molecular techniques such as FISH, PRTNS, PCR, RT-PCR, RFLP, LOH (lost of heterozygosity) became unavoidable in making accurate diagnosis. Therefore, fast, precise, accurate and reliable diagnosis of chromosome aberrations is possible only by combination of cytogenetics and molecular techniques.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

HETERODIMERIC PENICILLIN G AMIDASES - MOLECULAR MECHANIZAM OF POSTTRANSLATIONAL MATURATION OF INACTIVE PRECURSOR AND ITS BIOTECHNOLOGICAL IMPICATION

 

Goran Ljubijankić

 

Institute of Molecular Genetics and Genetic Engineering Vojvode Stepe 444a, PO Box 794, 11000 Beograd, Yugoslavia

 

The enzyme penicillin G amidase (penicillin G acylase, EC 3.5.1.11, PGA) catalyzes the hydrolysis of the acyl group of penicillin, yielding 6-aminopenicillanic acid, a key intermediate in the production of semisynthetic beta-lactam antibiotics. The main industrial source of the enzyme is Escherichia coli and that was a reason for intensive studies of this protein as well as the gene encoding it (pac). It appeared that E. coli pac gene has a specific structure which causes a synthesis of PGA as a single, biochemically inactive, polypeptide precursor. Its maturation occurs by removal of single peptide at the inner membrane, processing of the spacer peptide in the periplasm followed by formation of a biochemically active PGA composed of two different subunits (α approx. 23 kDa, and β approx. 65 kDa). The free (3 subunit carries N-tenninal serin which is a single amino-acid catalytic site. It was shown that a complex posttranslational maturation of PGA precursor was the limiting factor for a recombinant PGA overproduction in E. coli. Therefore, a investigation of the maturation process is not interesting only from a fundamental point of view, but also for industrial production of the enzyme. The following topics of our research will be discussed: the limited E. coli pac overexpression in yeast (a): the maturation as temperature-dependent process (b); characterization of a domain of precursor involved in this regulation (c); the proteolitical processing as an autocatalitic intermolecular mechanism (d).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STRUCTURAL ANALYSIS OF THE prt GENES REGULATORY REGION IN NATURAL ISOLATE Lactococcus lactis subsp. lactis BGIS29

 

Nataša Miladinov, Irena Spasojević i Ljubiša Topisirović

 

Institute of Molecular Genetics and Genetic Engineering, V. Stepe 444a, P.O.Box 794, 11001 Belgrade, Yugoslavia

 

Soj Lactococcus lactis subsp. lactis BGIS29 is a natural isolate from home-made cheese. The isolate BGIS29 produces the PI-type proteinase. Regulatory domain of the prt genes was sequenced. The prtP and prtM genes expression is controlled by divergent promoters PprtP and PprtM, respectively. Transcriptional start points of both prt promoters were determined. Sequence analysis also showed that putative secondary structure could be formed. Previous results have shown that the expression of the prt genes in BGIS29 is regulated by the presence of casitone in chemically defined medium (CDM). To evaluate the role which the regulatory region has on the prt gene expression, the influence of the nucleotide sequence context on DNA bending was investigated. Ligase-mediated cyclization and gel mobility experiments together with the computer-assisted prediction of a putative DNA bending are performed. Computer-assisted prediction of the DNA bending showed that a putative center of the bending is located in the vicinity upstream of the PprtP promoter. Gel mobility experiments revealed that the PCR fragment of 350 bp containing complete regulatory region is curved. The retardation value (R) was 1.2 in the absence of and 1.4 in the presence of the MgCl2. Moreover, truncated PCR fragment (270 bp) containing both, PprtP and PprtM promoters and center of banding retained bendability. Truncated fragment is lacking 80 bp located between the prtM gene and the center of banding. This fragment has R values 1.1 and 1.5 in the absence and presence of MgCl2, respectively.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INDUCTION OF ALBUMIN SYNTHESIS IN BELGRADE LABORATORY (b/b) RAT RETICULOCYTES

 

Zvezdana Popović, Aleksandra Đurković, Jelena Zarić i Marina Lušić

 

Institute of molecular genetics and genetic engineering, Vojvode Stepe 444a, P.O. Box 794, 11000 Belgrade, Yugoslavia

 

The Belgrade laboratory (b/b) rat is an autosomal mutant characterized by hemolytic anemia. An additional protein band of 68kD molecular mass, which corresponds to the rat serum albumin, characterizes b/b erythrocyte membrane. Albumin has been found in the cytoplasm of both, control (+/+, bled +/+ and phenylhydrazine treated +/+) and b/b erythrocytes. In b/b rat erythrocytes cytoplasm, amount of albumin is 5-7 times increased compared to the +/+ rat erythrocytes

Albumin synthesis is confirmed by immunoprecipitation of in vitro newly synthesized proteins with the specific polyclonal anti-human albumin antibodies. Albumin represents 35-40% of total proteins synthesized in b/b reticulocytes, 22-26% in reticulocytes of rat treated with phenylhidrazine and drops to 6-8% of total newly synthesized proteins in reticulocytes of bled rats.

Albumin synthesis in rat reticulocytes is further confirmed by the identification of albumin mRNA by RT-PCR method.

The present results confirm that albumin is synthesized, not only in liver but in rat reticulocytes, too. Albumin synthesis is inducible and increased in damaged (b/b and phenilhydrazine treated normal rat). Increased amount of albumin in damaged reticulocytes points to the specific function of albumin in stressed cells.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

HCV GENOTYPE DETECTION BY SSCP IN YUGOSLAV POPULATION

 

Stamenković Gorana, Laketa Danijela, Gudurić Jasenka, Veljković Emilija, Krtolica-Zikić

Koviljka i Dimitrijević Bogomir

 

Institut za nuklearne nauke "Vinča", Laboratorija za radiobiologiju i molekularnu genetiku,
Beograd, Jugoslavija

 

Hepatitis C virus (HCV) appears to be the major cause of chronic hepatitis (HCV). It has a positive single stranded RNA genome, which displays considerable variability precluding permanent immunity and the production of effective vaccine. On the basis of genomic sequence, HCV isolates are classified into six major genotypes and at least 15 subtypes. HCV genotypes play an important role in disease stratification and treatment modalities. These viral genotypes differ in their geographical distribution. Investigation of HCV genotype distribution in our region showed that genotypes Ib, 3a and la are the most frequent genotypes (90% of all analyzed samples). Genotyping was performed by RT-PCR method with type-specific primers which are complementary with specific sequences in core-region of HCV genome. However, genotyping based on the core segment yields frequently ambiguous results and/or are completely uninformative. On the other hand, the analysis the sequence of 5'-untranslated (NTR) region of the HCV genome also provides genotype specific information. SSCP mutational screening of NTR was optimized to yield a clear distinction between the genotypes in Yugoslavia. This is potentially valuable contribution to clinical diagnostics being suitable for routine screening and economically superior to available commercial tools.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BSA WITH CANDIDATE DROUGHT-INDUCED GENES

 

Vesna Lazić-Jančić, D. Kovačević i S.A. Quarrie

1 Maize Research Institute "Zemun Polje", Yugoslavia

2John Innes Centre, Norwich, UK

 

In response to drought stress, many genes are induced in plants and the question many research groups are trying to answer is to determine which of these genes have important roles to play in stressed plants. The approach normally adopted to determine the function of a gene is transformation and gene over-expression or silencing. However, interpreting the results of such studies is not always easy. An alternative approach is bulk segregant analysis (BSA). BSA using cDNAs for the gene as hybridization probes may give an answer to the question through examination of allele frequencies in two bulks of plants selected to differ specifically for the trait of interest.

We have recently used the amplified fragment length polymorphism (AFLP) technique for mRNA fingerprinting of two inbred maize lines differing in drought resistance and grown under drought stressed and control conditions. cDNA fragments prepared from leaves were identified that were specific either to a particular treatment or to a particular genotype or, in some cases, specific to a genotype only under one treatment. Using a range of primer combinations, over 100 fragments were identified and 14 of these have been cloned so far. Hybridizations to dot blots of mRNA confirmed that the majority of the cloned fragments were differentially expressed by either genotypes or treatments.

We are using BSA to screen the cDNAs that were differentially-expressed between treatments for differences in allele frequency between maize composites before and after several cycles of selection for high yield under drought conditions. Two pairs of composite populations are being tested: one pair produced entirely at CIMMYT, Mexico (Tuxpeno Sequia), and the other originating from CIMMYT and developed by the MRI in Zambia (DTP).

One AFLP-derived cDNA (M47/3C) showed allelic variation between the selected and unselected composites and this was mapped to the long arm of maize chromosome 8. We are now examining a mapping population of maize for coincidence of quantitative trait loci (QTLs) for drought responses and the M47/3C gene, and the results so far will be presented.

We are grateful to the Royal Society and UNESCO for providing financial support for this work.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

SOX GENES: BRAIN DEVELOPMENT AND SEX DETERMINATION

 

Milena Stevanović, Nikola Arsić, Tamara Rajić, Slavica Stanojčić, Tomislav Terzin, Vesna
Verbić, Maja Vujić, Nebojša Mirković i Danica Grujić

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia

 

The mammalian sex determining gene SRY encodes a protein with an HMG-box, a motif found in several DNA-binding proteins, including some transcription factors. The mammalian genome contains a family of genes related to SRY and homology is restricted to the region of SRY that encodes an HMG-box. These genes have been named SOX (SRY related HMG-box). The SOX family is large comprising at least 30 genes and by similarity within the HMG-box SOX genes are grouped in several subfamilies.

Although only few SOX genes have been characterized in details, increasing evidence indicates that SOX genes have important roles in the regulation of development and mutations in SOX genes are associated with human diseases.

SRY is the only member of the SOX gene family to b directly linked to the sex-determining pathway. Expression studies suggest that many SOX genes are embryonically expressed and expression patterns indicate mat some SOX genes might have roles both in controlling development of central nervous system and sex determination.

By analogy to SRY, SOX genes are expected to have important regulatory function and further analysis of this new family could lead to better understanding of processes of development of nervous system and sex determination.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

DETECTION OF B CHROMOSOME SPECIFIC DNA MARKERS IN

POPULATIONS OF YELLOW-NECKED MOUSE Apodemus flavicollis (RODENTIA, MAMMALIA)

 

Nikola Tanić1, Nasta Dedović2, Mladen Vujošević1 i Bogomir Dimitrijević2

 

1 Institute for Biological Research "Sinisa Stankovic", Belgrade

2 Institute for Nuclear Sciences''Vinca, Belgrade

 

The molecular structure of the B chromosomes of Apodemus flavicollis has been analyzed by DNA comparison of animals with and without B chromosomes using AP-PCR- based DNA profiling. Mice harboring one, two, three or none B-chromosomes were examined. Chromosomal stmcture was scanned for variant bands by a series of arbitrary primers and informative ones were selected. The selection criteria were their ability to differentiate between individuals of the species, to detect markers common for both A and B chromosomes and, importantly, to differentiate between A and B chromosome set. Along with primer, profiling conditions were found to be critical for meeting the criteria. Primers and conditions of the analysis that demonstrate structural characteristics unique to the B chromosome set are described. These characteristics include variant bands as qualitative parameters and altered electrophoretic band intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. Features of DNA profiles illustrate the reliable method of analysis of B chromosomes in the context of full set of A chromosomes. It provides an easy access to B chromosome specific molecular markers. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CONTROL OF RESISTANCE GENES IN ANTIBIOTIC-PRODUCING ACTINOMYCETES

 

Branka Vasiljević, Milorad Kojić, Nataša Milojević, Sandra Vajić, Marijana Miljković,

Tatjana Ilić

 

Institute of Molecular Genetics and Genetic Engineering, Belgrade

 

Actinomycetes have been the focus of much study because they produce a numerous medically important antibiotics. It is important to determine the mode of expression and regulation of genes governing resistance to antibiotics since those genes are usually clustered with biosynethetic genes. Concerning regulation of resistance genes in antibiotic producing actinomycetes, four different models have been described. The first model describes a complex regulatory cascade for biosynthetic genes in streptomycin-producing S. griseus. The model includes the positive A-factor signal which is transferred to strR, a regulatory gene. The resistance gene aphD is transcribed mainly by read-through from the A-factor-dependent strR promoter. The second regulatory mechanism is based on translational attenuation of MLS (macrolide, lincosamide and streptogramin) resistance genes or transcriptional attenuation (the resistance tlrA gene from the tylosin producer). The third mechanism of regulation of antibiotic resistance genes is demonstrated in a producer of novobiocin. The resistance gene from tins organism is driven by a promoter which responds to the superhelical density of DNA. The forth regulatory model is described in our laboratory and it is based on translational autoregulation. The aminoglycoside-resistance determinants from M. zionensis (sgm gene), and S. tenebrarius (kgmB gene) have been cloned and it has been shown that mechanism of resistance involves methylation of the 30S ribosomal subunit. The Sgm and KgmB methylases recognise specific sequences on 16S rRNA and also regulatory sequences on their own mRNA. In that way, if all ribosomes are modified, methylases bind to their own mRNA preventing further translation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE ISOLATION OF BUCKWHEAT GENOMIC CLONE CONTAINING THE GENE FOR METALOTHIONEIN-LIKE PROTEIN

 

Jelena Brkljačić1, Jelena Avramović1, Paja Šijačić1, Svetlana Radović2, Vesna Maksimović1

 

1 Institute for Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, Beograd,

2 Faculty of Biology, Studentski trg 3, Beograd

 

Metalothioneins are small molecular weight cysteine-rich proteins, with a capacity to bind metal ions. They are found in animals, fungi and bacteria, where they take part in different physiological processes. Animal genes for metalothioneins can be induced by a variety of stimuli, such as metal ions, hormones and secondary messengers, as well as by different kinds of stresses. Metalothionein genes are found also in plants, but only a few of them were characterized at the protein level. The data about elements involved in their expression are also scarce.

A cDNA clone pBM290, 600 bp long, has been isolated from cDNA library from buckwheat seed. The complete coding region for metalothionein-like protein has been identified in this clone. Based on Southern blot of buckwheat seed genomic DNA digested with restriction enzyme EcoRI, a fragment of 5 Kb (H2) was chosen as a template for asymmetric PCR. The primer, PREX290, was derived from pBM 290 clone sequence. To single-stranded DNA, obtained by asymmetric PCR, oligo-dG tails were added to 3'-end. Further amplification was performed with two primers - PREX290 and a NotC, which contains restriction site for NotI enzyme and oligo-dC tail. PCR products, 700 bp long, were ligated into the Bluescript vector and cloned in XL 1 Blue E.coli strain. Three clones, with sequences of a gene for metalotliionein-like protein were chosen on the basis of plasmid electrophoretic mobility, PCR amplification with PREX290 and NotC primers and hybridization with pBM290 probe. The final identity of these genomic clones will be confirmed by sequencing.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COMPARATIVE STUDY OF Micromonospora STRAINS PRODUCING AMINOGLYCOSIDE ANTIBIOTICS

 

Sandra Vajić, Milorad Kojić, Branka Vasiljević

 

Institute of Molecular Genetics and Genetic Engineering, Belgrade

 

Micromonospora strains which produce aminoglycoside antibiotics have high level of resistance to 4.6-disubstituted deoxystreptamine antibiotics that are structurally similar to their own products. The genes responsible for resistance in Micromonospora producers were cloned previously (the grm gene from M. purpurea, gentamicin producer, and the sgm gene from M. zionensis, producer of G-52). Both genes encode specific 16S rRNA methylases that modify target for aminoglycoside antibiotics and thus protect it. Southern hybridization experiments revealed that highly homologous genes are present also in other Micromonospora strains which produce aminoglycoside antibiotics. Genetic analyses of antibiotic producing organisms have revealed that in most cases the genes for resistance to antibiotics are clustered with their corresponding biosynthetic genes and, in some cases, with regulatory genes. This may indicate that expression of genes conferring resistance and biosynthetic genes are correlated ensuring that resistance is always operational when the organism is exposed to its own toxic product. Regulation of cloned sgm and grm resistance genes was studied. Although these genes are more then 80% identical in coding regions, their regulation is completely different. Therefore, it was interesting to analyse surrounding sequences, possible biosynthetic genes, for their homology. By hybridization with a DNA probe derived from pMK2 plasmid carrying the sgm gene on 7.2 kb fragment, chromosomal DNA of different aminoglycoside producers was analysed. Sequence analysis demonstrated that there is an open reading frame upstream of the sgm gene which show significant homology to the genes that encode aminotransferases, enzymes involved in biosynthetic pathways of different antibiotics.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE APPLICATION OF GENETIC MARKERS IN PREVENTION OF DAIRY CATTLE MASTITIS

 

Marijana Vučinić1, Z. Gajić1 i P. Stojić2

 

1 Faculty of Agriculture, Department for Livestock Production, Nemanjina 6, Zemun

2 PKB "INI Agroekonornik", Livestock Institute, Padinska Skela, Beograd

 

As many authors stressed, the most costly disease in dairy cattle of modern milk industry is mastitis. It is an inflammation of the mammary gland, usually caused by microbial infection in its one or more quarters. Protection of the mammary gland from microbial infection is governed by innate (nonspecific) and acquired (specific) immunity. Effectors of innate immunity are anatomical and physilogical inflammatory defense barriers, many antimicrobial substances, such as keratin, lactoferin. lysozyme. components of the complement, lactoperoxidase/thiocyanate/hydrogen peroxide system and phagocytic cells (macrophages. neutrophils and leukocytes). Effectors of acquired immunity are T- and B-lymphocytes and specific antibodies. Following microbial infection of the udder, large numbers of phagocytic cells enter the mammary gland. The number of these cells in milk is termed as Somatic Cell Count (SCC) or Somatic Cell Score (SCS) and practically serves as an indicator of alteration in the mammary gland secretion or its infection. The ability of phagocytes to ingest and kill microorganisms is a genetically regulated trait controlled by major histocompatibility complex genes (MHC genes). These genes encode histoglobulins and molecules on surface of leukocyte membranes. These molecules in cattle are known as Bovine Leukocyte Antigens (BoLA). Genes that encoded their synthesis (BoLA genes or MHC genes) influence immune response and disease resistance in cattle. Because, BoLA and Somatic Cell Score are potential genetic markers in selection of cattle to mastitis resistance.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

FUNCTIONAL ANALYSIS OF THE RAT βbminy - GLOBIN GENE PROMOTER

 

Nataša Grujičić, Sonja Pavlović, Sanja Pavlović, Zvezdana Popović

Institute for Molecular Genetics and Genetic Engineering, Vojvode Stepe 444A

P.O. Box 794, 11000 Belgrade, Yugoslavia

 

The rat β- globin gene locus is unique for its organization. It consists of either five or three adult β- globin genes depending on haplotype and we presume that transcriptional regulation of these genes within the locus has its own specific properties. The object of our investigation is the rat βbminy - globin gene promoter..

We have analyzed the regulation of transcription of the rat βbminy globin promoter using CAT functional assay. We have cloned 900 bp long promoter region from βbminy - -globin gene upstream of the CAT reporter gene. Promoter activity was investigated in rat erythroleukemia cell line (REL), a permanent cell line arrested at the proerythroblast stage of development. Upon treatment with DMSO, REL cells c m be induced to undergo erythroid differentiation in a way that closely mimics normal red-cell maturation.

Since we decided to analyze promoter activity in stable transfection assays, we also cloned HS3 (hypersensitive site 3) from human p-LCR in the reporter construct. The reason for this is that HS3 confers gene-copy-number-dependent expression of the linked gene in REL cells, independent of the site of integration in the host genome. We analyzed the activity of the rat βbminy -globin promoter in undifferentiated and differentiated stably transfected REL cells by measuring CAT activity. In mis experiment we showed induction of transcription from βbminy -globin promoter upon erythroid differentiation of REL cells.

In order to determine which regions of this promoter are functionally important for its tissue-specific transcriptional activation and inducibility we deleted certain parts of the promoter and began the analysis of the deletion mutants using CAT assay.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

SYNTHESIS AND SECRETION OF THE SGM METHYLASE IN Saccharomyces cerevisiae

 

Tatjana Ilić, Milorad Kojić, Goran Ljubijankić, Branka Vasiljević

 

Institute of Molecular Genetics and Genetic Engineering, Belgrade

 

The baker's yeast Saccharomyces cerevisiae was successfully used in many cases for die expression of heterologous genes. The aim of the present work was to clone the sgm gene to yeast expression vector pGoBO under yeast promoter and secretion signal. This approach offers the advantage for purification of the Sgm methylase from media.

The sgm gene was cloned from actinomycete Micromonospora zionensis, producer of an aniinoglycoside antibiotic, as resistant determinant. The gene encodes a specific methylase that modifies 16S rRNA. It was previously shown that the expression of this gene in different Streptomyces strains, in E. coli and in Micromonospora melanosporea gives resistance to gentamicin. It was interesting to test whether the sgm gene could confer resistance to gentamicin also in eukaryotic organism such as Saccharomyces cerevisiae. Computer analysis of 16S rRNA and 15S and 18S rRNA of yeast revealed that there is high homology in regions where gentamicin can bind. Therefore, we also tested the intracellular expression of the sgm gene. Such expression of the sgm gene in S. cerevisiae could lead to successful usage of this gene as dominant marker in yeast vectors with increased host range.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE REGULATION OF cls GENE EXPRESSION IN BACTERIA Escherichia coli K12

 

Radmila Janjušević, Mirjana Lilić, Milija Jovanović, Goran Jovanović i Dragutin Savić

 

Institute for Molecular Genetics and Genetic Engineering, V. Stepe 444a, P. Fah 794, 11001 Belgrade

 

            Escherichia coli cls gene codes for cardiolipin synthase, the enzyme that catalyses reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) and glycerol. CL is one of three major glycerophospholipides constituting biological membranes, but different from others for its tetraacyl structure and supposedly plays unique functional role. The membrane content of CL increases as E. coli cells enter the stationary phase. The specific activity of CL synthase is also about 10-fold higher in cells that are in the stationary phase than in those in exponential phase of growth. To examine cls gene expression strains containing operon (cls-lacZ+) and protein (els- 'lacZ) fusions were constructed. The same shape of curves of β-galaclosidase activities in different time points for operon and protein fusions indicate that the regulation of els gene expression is on transcriptional level. These results confirmed that the transcription of els gene increased when the cells enter the stationary phase. In addition, there is probably more than one regulatory factor involved in regulation of the cls gene transcription. The role of several E. coli global regulatory factors was examined by measuring β-galactosidase activity of an operon fusion in different regulatory mutants and by gel shift experiments. These results have revealed that some of global factors are also involved in the regulation of cls gene transcription.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CysB ACTIVATOR ACTS AS A REPRESSOR OF THE HslJ EXPRESSION THROUGH N-ACETYL SERIN MODULATED DNA BINDING IN THE hslJ LOCUS

 

Milija Jovanović, Mirjana Lilić, Radmila Janjušević, Goran Jovanović i Dragutin Savić

 

Institute for Molecular Genetics and Genetic Engineering, V. Stepe 444a, P. Fah 794, 11001 Belgrade

 

CysB protein belongs to the LysR family of regulatory proteins. It activates the
transcription of genes involved in the cysteine biosynthetic pathway as well as some other
genes such as
tauABCD, cbl, adi, lysU,. In turn, CysB negatively regulates expression of its
own gene. N-acetyl serin (NAS) is an inducer of the CysB activated transcriptions but anti-
inducer of the autogenously controlled cysB expression. We found that CysB protein
negatively regulates the expression of a new hslJ gene. In order to elucidate the regulatory
role of CysB implicated in expression of the HslJ. CysB protein with His tag on its C-
terminal region was purified near homogeneity. The functional activity of the CysB-His
fusion protein was confirmed in vivo. Analyses of gel retardation experiments employing
the purified CysB protein and the DNA fragment that comprises the regulatory region and
the 5' part of the hsU gene showed that in vitro, CysB specifically binds this fragment and
forms two complexes. Comparison of the results obtained using either the crude cell extracts
isolated from die wild type cells or the purified CysB-His revealed that CysB protein alone
binds this fragment, hence that no auxiliary proteins are involved in this binding.
Furthermore, we showed that NAS decreases the efficiency of the CysB-DNA binding in
the hsU locus acting as an anti-inducer. Therefore it might be that CysB plays a role of the
classical repressor of the HslJ expression while the NAS binding to CysB modulates this
negative regulation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CONSTRUCTION OF SHUTTLE VECTOR pA13

 

Milan Kojić, Slavica Arsenijević, Olivera Gajić, Vanesa Munćan i Ljubiša Topisirović

 

Institute for Molecular Genetics and Genetic Engineering, V. Stepe 444a, P. Fah 794, 11001 Belgrade

 

The smallest cryptic plasmid pAl (2820bp) of Lactobacillus plantarum A112 containing the RCR origin able to replicate in lactic acid bacteria (LAB) and E. coli was used for construction of shuttle cloning vector pA13. Vector pA13 was constructed by insertion of DNA fragment (1 kb) harbouring the erm gene from pIL253 and DNA fragment harbouring the lacZ(α) gene from M13mpl8 into pAl. Cloning DNA fragments in multiple restriction sites present in the lacZ(α) gene was facilitated by using blue/white selection of E. coli transformants. Further genetic manipulation was allowed by selection of Emr transformants in both LAB and E. coli. Results showed that vector pA13 posses high efficiency of transformation of a LAB and had a broad host range. In addition, it had pretty good stability, i.e. segregation is relatively low. The pA13 vector was used for cloning bacteriocin genes from Lactococcus lactis BGMN1-5 by making the library of bacteriocinogenic plasmid in E. coli. Recombinant clones containing the bacteriocin genes was isolated by transformation of bacteriocin-sensitive strain L. lactis IL1403 and selection for bacteriocin production and immunity. The pA13 vector and recombinant clones with bacteriocin genes were stable maintained in LAB and E.coli. Vector was also used for cloning of heterologous sgm gene (GC reach) from Micromonospora zionensis. Results showed that recombinant clones were stable in LAB as well as in E. coli. However, the expression of the sgm gene was obtained in E. coli but not in LAB.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

LARGE SCALE OF ISOLATION OF RAT ERYTHROID PROGENITORS FOR GENE THERAPY

 

Marija Mijatović1, Jelena Stanković2, Marijana Petakov2, Gordana Jovčić2, Jelena Zarić1, Zvezdana Popović1

 

1Institute for Molecular Genetics and Genetic Engineering

2 Institute for Medical Research

 

Hemopoiesis is studied in vitro mainly in semisolid cultures, where hemopoietic progenitors develop into discrete colonies. The fact that the cells are immobilized in the semisolid medium makes difficult or impossible to carry out analysis of growth kinetics of the developing cells.

We describe a liquid culture system that supports the growth and maturation of rat erythroid progenitors. The procedure was divided into two phases. In the first phase mononuclear cells from the peripheral blood and bone marrow were cultured in a density of 5x106 cells/ml in alpha minimal essential medium supplemented with 10% PCS, 10% PKW-SCM and 1 μg/ml cyclosporin A. The cultures were incubated at 37°C in an atmosphere of 5% CO2 in air with extra humidity.

Following a 3 days of incubation in these primary cultures, the cells were harvested, washed and recultured in alpha medium containing 30% PCS, 1% BSA, 10-5 β-mercaptoethanol and 1U/ml Epo. These secondary cultures were incubated at 37°C in an extra-humid sealed incubator flushed with a mixture of 5% O2, 7% CO2 and 88% N2.

In the first phase (Epo independent) cultures showed a sharp decrease in absolute number of erythroid colony-forming progenitors. During the second phase, the presence of Epo, a high concentration of PCS and incubation under low oxygen pressure resulted in the development of erythroid cells. This type of liquid culture can give higher cell yield with quite synchronous cell differentiation stages and easily be applied, as particularly suitable target cells, in gem therapy protocols.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

TWO-PLASMID SYSTEM FOR ISOLATION OF REGULATORY AND GENTAMICIN SENSITIVE MUTANTS OF THE SGM GENE

 

Marijana Miljković, Nataša Milojević, Milorad Kojić, Branka Vasiljević

Institute of Molecular Genetics and Genetic Engineering, Belgrade

 

The sgm (sisomicin-gentamicin resistance methylase) gene is resistant determinant cloned from aminoglycoside producing strain Micromonospora zionensis. It encodes specific 16S rRNA methylase which protects ribosomes from inhibitory effect of aminoglycoside antibiotics. It was shown previously that the sgm gene is regulated at the translational level by negative autoregulation. Namely, Sgm methylase- binds to specific regulatory sequence in front of ribosomal binding site on its own mRNA and thus prevents its own further translation when all ribosomes are methylated (protected). Regulation of sgm gene was studied by use of sgm-lacZ fusion system. In order to study regions responsible for autoregulation or/and methylation of ribosomal RNA, a system with two plasmids was developed. The sgm-lacZ fusion was cloned on a low-copy plasmid pMS42l and cells carrying such construct were transformed with in vitro mutagenized plasmid carrying the sgm gene. Screening procedures were designed in a way that either regulatory or gentamicin sensitive mutants could be detect. Results of analysis of obtained mutants will be presented.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PROMOTER SEQUENCES REQUIRED FOR REGULATED EXPRESSION OF THE RAT βbminy - GLOBIN GENE IN REL CELLS

 

Tatjana Mitrović i Zvezdana Popović

 

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia

 

The rat β-globin locus is unique for its organization. It consists of three to five adult genes depending on haplotype. We have shown that one of these genes - βbminy is transcriptionally active in rat reticulocytes. Further, we try to elucidate the transcriptional regulation of βbminy -globin gene during erythroid differentiation. Our study is based on D5A1 REL (rat erythroleukemic) cells, homologous model system, which can successfully mimics in vitro the events of normal erythropoiesis. In order to evaluate the contribution of promoter sequences to transcriptional regulation of βbminy - globin gene, we have made hybrid promoter-CAT reporter gene constructs. The expression of these reporter plasmids was confirmed by primer-extension analysis. On the basis of stable-transfection experiments, we could define the minimum elements which are required for enhancement and induction of transcription in differentiating REL cells. We have shown that the presence of -96 bp promoter region (TATA-βDRE-CCAAT-CACCC) is sufficient for erythroid specific expression of βbminy - globin gene.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POLYMORPHISM OF PCR BASED MARKERS AND HETEROSIS IN MAIZE

 

Snežana Mladenović Drinić i Kosana Konstantinov

Maize Research Institute, 11080 Zemun Polje-Belgrade

 

As parental genetic divergence has been found to increase the potential for heterosis in crosses, it is suggested using of indirect measures of genetic diversity as possible predictors for the heterotic response of hybrids (polymorphism of molecular markers). The five maize inbreds (F2, ZP120, ZP 2039, W 401, EP1) and 10 hybrids were evaluated for grain yield. Heterotic effect was calculated regarding the mid-parent value and estimated heterosis for yield according Eberhart and Gardner, 1966. The parental lines were surveyed for polymorphism with randomly chosen set of 58 SSR loci and 15 AFLP primers pair resulting in a total of 1306 reproducible amplification products, 595 of them being polymorphic. Roger's genetic distance were computed for all ten combination of the five parents based on 595 marker variants (501 AFLP variants, 94 SSR allele variants) and ranged from 0.618 in cross F2 x ZP2039 to 0.841 in cross ZP120 X W401 with an average of 0.682 across all ten pairs. The coefficient of correlation between genetic distance based on AFLP/ SSR marker date and mid-parent heterosis for yield was positive and significant 0.604/0.687 and with estimated heterosis 0.578 / 0.636, respectively. The coefficient of correlation between AFLP and SSR marker date is 0.752. The results indicated that genetic diversity among parental lines is certainly related to heterosis.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

YEAST SHUTTLE EXPRESSION VECTOR WITH DOMINANT SELECTIVE MARKER

 

Pavković, N., Todorović, V., Glišin, V., Ljubijankić, G.

Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, Belgrade

 

Yeast Saccharomyces cerevisiae is one of the standard expression system in genetical engineering which shows many good features of prokaryotic expression systems and also possess mechanisms of postranslational modifications inherent to higher eukaryotic cells.

Shuttle vectors pBLITZ has been obtained by fusing selective markers and replication elements of both S. cerevisiae and E. coli. pBLITZ contains sequences responsible for replication (ORI) and maintenance of high copy number (STB, FRT, FLP) from naturally occurring yeast plasmid 2>im, E. coli plasmid pBR322 origin of replication, and selectable markers: E. coli β-lactamase gene (conferring ampicilin resistance) and auxotrophic marker LEU2 from S. cerevisiae genome. pBLITZ also contains BLITZ expression cassette that allows expression and secretion of wide range of heterologous proteins in yeast.

An obstacle for wider usage of this plasmid is lack of dominant selectable marker-gene which would increase the range of host strains and allow selection in rich media. Therefore a gene for aminoglycoside antibiotics resistance (kanamycin, geneticin...) Tn903 kanr functional in both E. coli and S. cerevisiae was cloned.

New shuttle vector with dominant selective marker has been utilized for 'construction of new plasmids for expression and secretion of heterologous proteins from prokaryotes (penicillin amidase; plasmid pGoB-2KS), and eukaryotes (human interferone-β; plasmid pG1FN).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

LOCALISATION OF CONTROL ELEMENTS RESPONSIBLE FOR SOX3 GENE ACTIVATION BY RETINOIC ACID

 

Tamara Rajić, Nikola Arsić, Nebojsa Mirković. Milka Sokolović and Milena Stevanović

 

Institute of Molecular Genetics and Genetic Engineering. Vojvode Stepe 444/A P.O. Box 794, 11000 Belgrade, Yugoslavia

 

Retinoids have been shown to have effects in a variety of biological processes including cell differentiation, embryogenesis and neoplasia. Retinoic acid (RA), the natural acidic derivative of vitamin A, seems to be of major importance in many aspects of CNS development. Effects of both vitamin A excess and deficiency on the developing embryos of mammals are associated with CNS defects.

SOX3 gene is a member of new family of genes, which are embryonicly expressed, and the expression is restricted to neuronal and related tissues. Expression patterns of SOX3 gene and mentally retarded patients, deleted for this gene, suggest certain role in the regulation of development of CNS.

SOX3 gene is activated by RA and the sequence responsible for RA activation resides within 400 bp fragment. Five overlapping subfragments 200-334 bp in length responsible for RA activation are made by PCR, cloned in pBLCATS vector and linked to the CAT (Chloramphenicol Acetyl Transferase) reporter gene. These constructs after transient transfection into NT2/D1 cells were assayed for the CAT activity in the presence and absence of RA.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MOLECULAR-GENETIC ANALYSIS OF TEMPERATURE-DEPENDENT POSTTRANSLATIONAL MATURATION OF E. coli PAC

 

Spasić M., Glišin V., Ljubijankić G.

 

Institute of Molecular Genetics and Genetic Engineering, V. Stepe 444a, Beograd, Yugoslavia

 

Penicillin G acylase (PAC) catalyses hydrolysis of penicillin G to 6-aminopenicillinic acid and phenilacetic acid (PAA), which marks this enzyme as very important for the semi synthetic antibiotics production.

Regulation of Escherichia coli pac expression is complex and encompasses different mechanisms both on transcriptional and posttranslational level. In order to acquire functional, heterodimeric enzyme, nascent PAC precursor has to undergo proteolytical processing, which represents limiting step in industrial synthesis of the enzyme both in bacteria and yeasts. In E. coli this processing takes place only below 30°C. However, Providencia rettgeri pac gene (62% homology with E. coli pac on aminoacid level) is expressed in temperature-independent manner.

In order to determine a region responsible for this difference, we constructed a recombinant plasmid carrying both pac genes. This vector, introduced into E. coli, was subjected to intraplasmid homologous recombination between two pac genes which allowed us to isolate a number of chimeric pac genes.

Further analyses encompassed clones that posses significantly changed characteristics of posttranslational processing of precursor: (1) a chimeric gene which possess temperature-independent precursor processing and (2) a group of recombinants with lowered efficiency of posttranslational maturation.

We have determined exact nucleotide positions of recombinational events by restriction analysis, PCR mapping and sequencing. That allowed us to determine region(s) that influence thermal dependencies of posttranslational processing in E. coli precursor. Our further work was aimed at precise delimiting of these regions, using technologies of genetic engineering.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MOLECULAR ANALYSIS OF THE prt GENE REGULATORY REGION OF NATURAL ISOLATE Luctobacillus paracasei subsp. paracasei BGHN14

 

Irena Spasojević, Nataša Miladinov i Ljubiša Topisirović

 

Institute for Molecular Genetics and Genetic Engineering, V. Stepe 444a, P.O.Box 794, 11001 Belgrade

 

Lactobadllus paracasei subsp. paracasei BGHN14 is natural isolate from homemade cheese. Preliminary analysis revealed that this strain produces the proteinase similar to the lactococcal PI-type proteinase. Hybridisation experiments as well as restriction analyses indicated that proteinase gene region have similarity with that of lactococci. In contrast to lactococci, prt genes of lactobacilli are chromosomal located. Proteolytic activity of the BGHN14 is depended on concentration of casitone in basal minimal medium (BMM). Higher casitone concentrations (1.0% and 2.0%) exhibited inhibitory effect upon the BGHN14 proteinase activity. Regulatory domain of BGHN14 was subcloned into vector pNZ273 resulting in construct pHN174, and sequenced. Results revealed that this region contains both PprtM and PprtP promoters but is shorter for 35 bp in comparison to that of lactococci. The nucleotide sequence missing in BGHN14 is forming potential steam-loop structure suspected to be involved in casitone-dependent regulation of the prt genes expression in Lactococcus lactis SKI 10, Wg2 and BGIS29. However, testing the p-glucuronidase activity in L. lactis NZ9000/pHN174 tran-formant in which the gusA gene is under control of the Pprtm promoter of BGHN14 revealed that casitone mediated regulation was at the transcriptional level.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PRELIMINARY CHARACTERISATION OF THE THREE NOVEL HUMAN SOX GENES

 

Slavica Stanojčić, Maja Vujić, Nikola Arsić, Tamara Rajić i Milena Stevanović

 

Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/A, P.O. Box 794, 11000 Belgrade, Yugoslavia

 

The mammalian genome contains a family of genes that are related to SRY, the mammalian sex determining gene, and these genes are called SOX genes (SRY-related HMG-box genes). By analogy to SRY, these genes might be expected to play a role in the regulation of development and sex determination.

Preliminary results suggest that many SOX genes are embrionically expressed and expression patterns indicate that some of them might play some role in development of central nervous system.

A new human SOX gene, named SOX 14, has been isolated from the human genomic library. This gene has been mapped to human chromosome 3q23 region. SOX 14 HMG-box amino acid sequence shares 65,8% similarity to HMG-box of the SRY gene, and 100% similarity to HMG-box of mouse Soxl4 gene. It is predominantly expressed in foethal spinal cord, foethal brain and foethal thymus.

The other two human SOX genes, named SOX18 and SOX20, have been isolated from human foethal cDNA library. One of them, named SOX 18, shares 52,6% of amino acid similarity to HMG-box of the SRY gene and 74% amino acid similarity to amino acid sequence of mouse Sox18 gene. SOX18 has been assigned to chromosome 20q13.3 region using radiation hybrids.

The other one named SOX20 gene, shows 65,8% amino acid similarity to HMG-box of the SRY gene, and it does not appear to be a true homologue of any of known mouse Sox genes. SOX20 has been assigned to chromosome 17pl3 region using FISH and radiation hybrids. The gene is widely expressed in foethal and adult human tissues. Further analysis of these genes will provide information about their potential roles in controlling developmental processes in humans.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENOTYPING THE BOVINE TRAITS OF ECONOMIC INTEREST

 

Slavica Stanojčić, Jelena Đurović, Nikola Arsić, Nebojsa Mirković, Vesna Verbić, Milena Stevanović

 

Institute of Molecular Genetics and Genetic Engineering. Vojvode Stepe 444a P.O. Box 794, 11000 Belgrade, Yugoslavia

 

 

Caseins are predominant (80%) proteins in bovine milk. One of them, K-casein is polymorphic and the most frequent variants are A and B. The K-casein allele B is more desirable for cheese production. Milk obtained from cows with BB genotype shows higher curd firmness and higher cheese yield.

Bovine leukocyte adhesion deficiency (BLAD) is a lethal autosomal recessive disease. It is caused by point mutation in CD 18 gene. This gene encodes p subunit of glycoprotein which is responsible for adhesive interactions between leukocytes and infectious agents. The point mutation causes an amino acid substitution that makes this glycoprotein inactive. Affected animals suffer from frequent infections which recur after conventional treatment, and they die before reaching maturity.

For fast and precise detection of gene variant for K-casein and BLAD, we've introduced a DNA test based on polymerase chain reaction (PCR) and RFLP. Test included amplification of specific region of CD 18 gene, exon 4 of K-casein, which contains polymorphic sequence and later treatment of these fragments by restriction enzymes.

This PCR-RFLP DNA test enabled us to detect normal and defective CD 18 allele, to identify affected, normal and heterozygous animals and also specific allele for K-casein they had.

After analysis of CD 18 sequence and sequence of K-casein gene, we've designed oligonucleotides which have been used as primers. We have obtained fragments of expected lengths by multiplex PCR. After precipitation, PCR products were treated with restriction enzymes PstI and Hae III. Then, after double digestion, the fragments corresponding to each genotype were easily detected in 4% agarose gel.

Genotypes based on polymorphic restriction sites for both characteristics can be easily detected from banding pattern what make the test useful for the selection of traits of economic interest.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ANALYSIS OF CONJUGATIVE ABILITIES OF PLASMIDS IN Lactococcus lactis subsp.

lactis biov. diacetylactis S50

 

Ivana Strahinić, Milan Kojić i Ljubiša Topisirović

 

Institute of Molecular Genetics and Genetic Engineering, V. Stepe 444a, P.O.Box 794, 11001 Belgrade

 

Lactococcus lactis subsp. lactis biovar. diacetylactis S50 is a natural isolate from starter culture for butter production. The strain S50 contains five plasmids, in range from 6.7 kb to about 300 kb (pS50-290, pS50-80, pSSO-lOa, pSSO-lOb and pS50-7). This strain produces bacteriocin with narrow antibacterial spectrum, bacteriocin S50 and cell wall-asociated proteinase of PI-type located on the large plasmid pS50-290. Other plasmids are cryptic. The strain S50 was used as a donor in conjugation experiments for transfer of plasmids to L. lactis MG1363 and IL1403 and S50 derivatives obtained by plasmid curing. The transconjugants from these crosses contain plasmid pS50-290 but also small plasmids pS50-10a, pS50-10b and pS50-7 in various combinations. These transconjugants were used as donors in further conjugations, but transfer of small plasmids was not detected. Results also showed that only plasmid pS50-290 could be transmitted in these crosses. Taking all these results together, it appeared that plasmid pS50-80 was responsible for cotransfer of small plasmids during conjugation. Hybridisation experiments revealed that plasmids pS50-7, pS50-10a and pS50-10b contain homologous nucleotide sequences with plasmid pS50-80. Homologues TaqI DNK fragments were cloned from all four plasmids and sequenced. Comparative analysis of sequences revealed that they share high degree of homology among themselves. In addition, they contain homology with oriT region of the well-known lactococcal plasmid pCI528.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CLONING OF MOLE RATS Sox2 GENE USING PCR METHOD

 

Tomislav Terzin, Milka Sokolović, Ana Basić i Milena Stevanović

 

Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/A, P.O. Box 794, 11000 Belgrade, Yugoslavia

 

Cloning a gene for determination male sex in mammals (Sry), led to determination a whole family of related genes. Homology between them is restricted to HMG box; therefore this genes were named Sox genes (Sry related HMG box).

Sox genes are expressed during embrional development, in neural and related tissues and undifferentiated gonads. Therefore it is predicted that Sox genes are transcriptional factors that have a role in development of central nervous system and sex determination. Using PCR based approach a large number of Sox genes have been identified in different animals. Apart from mammals, Sox genes have been identified in birds, reptiles, amphibians, fishes, insects and nematodes.

Research has been shown that Sox2 gene is involved in controlling of eye development. The aim of this research is to clone and characterize, Sax2 gene from mole rat (Spalax leucodon). Mole rats eyes are rudimentary and they can distinguish only light from darkness. Because of the role of Sox2 gene in eye development this gene will be cloned and analyzed for presents of possible mutations in mole rat.

Primers specific for 5' and 3' region of human SOX2 gene are used to amplify Sox2 gene from mole rat. PCR products are cloned. By hybridization with box and SOX2 specific probe, it is shown that cloned fragment of mole rat is indeed Sox2 gene. Hybridization analysis indicate high conservation of Sox2 gene in mole rat and human. Confirmed high conservation of Sox genes makes possible to analyse the animal Sax genes using primers corresponding to human SOX genes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BIOCHEMICAL AND GENETIC CHARACTERIZATION OF STAPHYLOCOCCAL EXTRACELLULAR PROTEINASES

 

Đorđe Fira i Ljubiša Topisirović

 

Institute for Molecular Genetics and Genetic Engineering. V. Stepe 444a, P.O.Box 794, 11001 Belgrade

 

A collection of natural isolates from bacterial genus Staphylococcus is obtained from food samples, which originate from different geographical locations. The isolates have been screened for production of extracellular proteinases. Amongst 171 isolates, 55 of them were found to be proteolytically active, since their cultures proteolytically coagulate milk. Specificity of staphylococcal extracellular proteinases towards casein fractions has been analysed by electrophoresis. In the process of milk coagulation, staphylococcal proteinases, beside p-casein, significantly hydrolyzed as]- and K-casein. Biochemical characteristics of proteinases from isolates F22, F86, M104, S2007 and S2105 have been studied in detail. It was found that these proteinases have relatively low molecular masses (from 20 to 32 kDa), an that they are released from the cell envelope in the growth medium spontaneously. Their temperature optima are between 30°C and 37°C and their pH optima ranging from 6.5 to 8.7. Copper ions inhibit, but the presence of calcium ions stimulates the activity of proteinases of isolates F22, M104 and S2007. Beside casein fractions, they also hydrolyze heterologous protein substrates, such as BSA and gelatin. Experiments with specific proteinase inhibitors revealed that proteinases of isolates F22 and M104 belong to the serine group of proteinases. The S2007 and S2105 proteinases were classified as metaloproteinases whereas the type of F86 proteinase in these experiments could not be determinated. In hybridization experiments, total DNA isolated from these isolates did not hybridize to the lactococcal proteinase genes probes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MECHANISM OF POSTTRANSLATIONAL PROCESSING OF THE HETERODIMERIC PAC

 

Ševo M., Glišin V. i Ljubijankić G.

 

Institute of molecular genetics and genetic engineering, Vojvode Stepe 444a, Belgrade

 

Bacterial enzyme penicillin G acylase (PAC) catalyses the hydrolysis of the acyl group of penicillin G. In bacterial cell PAC is synthesised as a single inactive cytoplasmic precursor (pre-pro-PAC). Maturation process begins by removal of signal peptide on inner membrane, followed by release of the spacer peptide and the formation of biochemical} active PAC composed of two unequal-size subunits (α and β) in the periplasm. Synthesis of active enzyme due to expression of the pac gene not only in different Gram-negative bacteria (Sizmann et oi, 1990) but also in yeast (Ljubijankic, 1995) excluded involvement in reaction of speciffic as well as unspeciffic endopeptidases, suggested that the spacer peptide release might be an autocatalytical event. To confirm this hypothesis we constructed the E. coli pac mutant gene (Δ 20 pac) that contains a deletion of the last 20 codons from the 3' terminus and encodes a biochemically inactive precursor molecule. This mutant gene was in vivo coexpressed with the intact pac gene (wt) from Gram-negative bacterium Frovidencia rettgeri in E. coli strain DH5α. Coexpression of these genes was monitored by Serratia marscescens overlay technique and by NIPAB colorimetric assay, and we observed a significant change in the whole PAC activity of the cells. Results of the periplasmic proteins analysis suggest an intermolecular interaction of two different PAC precursors in the process of autocatalytic posttranslational processing.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE ISOLATION OF GENOMIC CLONE CONTAINING THE GENE FOR A SUBUNIT OF BUCKWHEAT SEED STORAGE PROTEIN

 

Paja Šijačić1, Jelena Brkljačić1, Svetlana Radović2, Vesna Maksimović1


1
Institute for Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, Beograd
2
Faculty of Biology, Studentski trg 3, Beograd

 

Buckwheat seed storage proteins contain three types of proteins: 13 S legumin-like globulins, 8S vicilin-like globulins and 2S albumin-like proteins. A cDNA clone pBF349, with an insert of 600 bp, has been isolated from a cDNA library from buckwheat seeds in mid-maturation stage. This insert has been shown to be a part of the gene for basic polypeptyde subunit of legumin-like storage proteins.

Based on Southern blot of buckwheat seed genomic DNA digested with restriction enzyme EcoRl, fragments 6-9 Kb (HI) were chosen as a template for asymmetric PCR with M9 primer, derived from pBF 349 clone coding sequence. To single-stranded DNA, obtained by asymmetric PCR, oligo-dG tails were added to 3'-end. Further amplification was performed with two primers - M9 and a NotC, which contains restriction site for NotI enzyme and oligo-dC tail. PCR products, 600 bp long, were ligated with the Bluescript vector and cloned in XL 1 Blue E. coli strain.

Four clones, containing sequences of a gene for polypeptyde subunit of legumin-like buckwheat storage protein were chosen on the basis of plasmid electrophoretic mobility, PCR amplification with M9 and NotC primers and hybridization with pBF349 probe. The final identity of these genomic clones will be confirmed by sequencing.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

KARYOTYPE ANALISIS AND CHROMOSOME NUMBER VARIABILITY IN

THE FISH SPECIES Cyprinus carpio L. I Carassius auratus gibelio B. (Cyprinidae, Pisces)

 

Svetlana Fišter

 

Faculty for Veterinary Medicine, University of Belgrade, Bul. JNA 18, 11000 Belgrade

 

The fish species Cyprinus carpio L. and Carassius auratus gibelio B. (Cyprinidae) are of tetraploid origin. According to some data from the available literature, the chromosome number in the both species in the natural bisexual population varies from 98 to 104.

The studies of wild type carp from the Danube population indicate that the diploid chromosome number was 2n=104 in the all individuals examined. There were no the individuals with 2n=98, or 2n=100 chromosomes, as some authors were reported for the carp from some waters. Karyotype of this carp consists of: 24 metacenmcs (M), 12 submetacentrics (SM), 24 subacrocentrics (SA) and 44 acrocentrics (A). The fundamental arm number (NF) equals 164.

In the fish species Carassius auralus gibelio B., in accordance with the existing data, the diploid chromosome number varies from 98 to 104, too. The bise.xuals and the gynogenetic Iryploid lines of this fish species exist in nature. Populational-genetic studies in the fishes from Yugoslav waters indicate to the chromosome number that varies among individuals in the bisexual and among gynogenetic populations. The existence of bisexual populations was affirmed in Yugoslav waters in 1989 and 1991. The basic diploid chromosome number in the fishes from Danube was 2n=100. but some differences occurred in the number of tiniest acrocentrics. The basic karyotype of 2n=100 consists of: 24 metacentrics (M). 12 submetacentrics (SM), 24 subacrocentrics (SA) and 40 acrocentrics (A). The fundamental chromosome arm number (NF) equals 160.

Variations in the number of tiniest acrocentrics in the both species probably occurred as a result of ancestral letraploidisation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE INCLUSION OF POPULATION OR SPECIES CHROMOSOME VARIANTS IN THE COMBINATION POTENTIAL OF AN INDIVIDUAL

 

Boro P. Pavlović

 

Institute of Forestry, Kneza Viseslava 3, Belgrade, Yugoslavia

 

There are quite simple relations between the number of variants of haploid (V) and diploid chromosome complements (W, K) depending on the number of chromosomes in the haploid complement (n) and on the number of variants of each member (Vj) of the chromosome set presented in the group of individuals, in the population or species. Maximal potential of a diploid individual is V=2n of gamete variants and K=3n of diploid variants within the basic set W=22n of progeny variants. These relations can be used in the explanation of a wide specter of issues: origin and conservation of biodiversity, optimization of chromosome matterial partition, variation potential intra species, population or cultivated and conserved live stocks. Special attention has been devoted to the issue of minimal number of individuals that can include the maximal number of chromosome variants. This can be applied to the solving of the problem of the scope of variation contained in Noah's Ark. spaceship voyage plans or biodiversity salvage in catastrophes, but also in the conditions of everyday activities of the modem civilization. A simple and surprising finding is mat the minimal number of individuals (which can cover the maximal number of chromosome set variants) depends only on the number of variants of the maximally variant chromosome (vmax). This minimal number of diploid individuals is equal to half a number of variants of that chromosome, vmax/2, for an even, or (vmax+l)/2, for an odd number of chromosome variants. The problem of the conservation of population and species genetic diversity should be based on the study of the aspect of combinations of chromosome variants.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC LOADS AND COADAPTIVITY OF CHROMOSOMAL INVERSION POLYMORPHISM IN A DROSOPHILA SUBOBSCURA

 

Goran Živanović i Marko Anđelković


Department of Genetics, Institute for Biological Research "Sinisa Stankovic", Belgrade

 

The most attractive way to detect the coadaptivity of chromosomal inversion polymorphism in natural populations of Drosophila might be studies on the level of the genetic load. Considering the extremely rich inversion polymorphism of Drosophila subobscura populations of this species might serve as suitable model-systems to study the connection between these two kinds of genetic polymorphism. Taking these facts into consideration an analysis was done of the inversion polymorphism and genetic loads of the O-chromosome in three populations of Drosophila subobscura sp. Observed data showed that in all three populations differences exist in the average viabilities of O-chromosome gene arrangements. The differences observed are a consequence of the uneven distribution of different viability classes among different O-chromosome gene arrangements. The uneven distribution is neither limited to the lethal viability class, but is characteristic to viability classes from the group of quasi normal’s too. The results obtained on the relation of the extent of the genetic loads and the frequencies of gene arrangements showed that the gene arrangements with a moderate and high frequency have the highest viability, i.e. the lowest extent of the genetic load. Observed results also showed that more complex gene arrangements (O3+4, O3+4+i) had the higher viabilities values than gene arrangement without inverted regions (Ost). These results point to high degree of coadaptivity of chromosomal inversion polymorphism in Drosophila subobscura.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

DOES THE PRESENCE OF B CHROMOSOMES AFFECT TAXONOMIC DETERMINATION IN THE GENUS APODEMUS (RODENTIA, MAMMALIA)?

 

Jelena Blagojević i Mladen Vujošević


Institute for Biological Research "Sinisa Stankovic", Belgrade

 

The presence of B chromosomes in the yellow-necked mouse Apodemus flavicollis was analyzed in 320 adult animals from four localities in Yugoslavia. Our aim was to assess the effects of B chromosomes on certain morphometric characteristics. The characters that were chosen are included in the taxonomic discrimination of this particular species as well as the sibling species Apodemus sylvaticus. B chromosomes were found in all localities in frequencies raging from 21.6% to 42.9%. It was established that the relative length of the tail (the body/tail ratio) was significantly increased in the presence of B chromosomes. This index demonstrates geographic variability, however, in general the studied specimens had longer bodies than tails. It was ascertained that different frequencies of animals with B cliromosomes change the slope of the regression curve of the length of foramina incisiva (FI) relative to the distance from the 3rti upper molar to the incisor (IM3). This finding questions the validity of use of this relative value in taxonomy without taking into consideration karyological data.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

Fraxinus ornus L., SEXUAL POLYMORPHISME AND RAPD MARKERS

 

S. Bojović, Ph. Heizmann# M. Barbero*

 

Srbijašume - Institute of Forestry, Kneza Višeslava 3, 11030 Beograd

tel: 553 355, e-mail: bojos@ptt.yu

#Reconnaissance Cellulaire et Amélioration des Plantes, UMR 9938-CNRS-INRA. Ecole

Normale Supérieure-Lyon. 46 allée d'ltalie, 69364 Lyon, France

*Institut Méditerranéen d'Ecologie et de Paléoécologie. CNRS-UPRES A 6034, Av.

Escadrille Normandie Niémen, 13397 Marseille, France

 

Flowering (or Manna) ash (Fraxinus ornus L.) is a mainly a Mediterranean tree species of wide ecological amplitude. In terms of floral biology Fraxinus ornus L. shows an extremely rare form of sex expression - androdioecy; defined by a simultaneous presence of male and hermaphrodite flowers on separate individuals (presence of male and hermaphrodite individuals in populations). It is a rare and original system, both from theoretical and from empirical points of view. The possible sex differentiation between male and hermaphrodite individuals was examined by PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) molecular genetic markers.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INTRA-AND INTER-POPULATION VARIABILITY IN HARE (Lepus europaeus PALLAS)

 

Ljiljana Vapa, Dragana Obreht, Milan Vapa*, Deana Demjen

 

Institute of Biology, Trg D. Obradovića 2, 21000 Novi Sad.

*Faculty of Agriculture, Trg D. Obradovića 8. 21000 Novi Sad

 

One important aspect in the complex field of genetic resources conservation is measuring of amount and distribution of genetic variation withir populations and species of wild animals. Attention should be paid to the loss of alleles caused by selective hunting, founder effect, genetic drift, poaching and reintroduction of species. In order to evaluate genetic variability in hare in northern Vojvodina, samples from four populations were analyzed by standard polyacrilamide gel electrophoresis. Twenty-three isozyme loci were analyzed, and only 8 of them were polymorphic. It was found that average heterozygosity was H=0.107 and polymorphism was P=0.043, suggesting high rate of intra and inter population homogeneity. The gene erosion is a main problem in game breeding and presented type of analysis could serve as a method for monitoring of genetic diversity.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GLIADIN ALLELIC VARIATION AND DIVERSITY OF AGRONOMIC PROPERTIES IN WHEAT

 

Dimitrijević Miodrag1, Knežević Desimir2, Petrović Sofija1

 

1 Institute of Field and Vegetable Crops, Faculty of Agriculture, Novi Sad

2 ARI SERBIA, Small Grains Research Center, Kragujevac

 

The sample of 77 wheat varieties was analyzed for presence of gliadin alleles Gli Bib. Gli B11, Gli D1b. and Gli D2b. Agronomic trait differences were compared, testing examined genotypes grouped due to presence or absence of these gliadin alleles in the genetic background.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

"AFILA" GENE EFFECTS ON ABSOLUTE SEED MASS IN PEAS (Pisum sativum L.)

 

Đorđević Radiša, Zdravković Milan, Zečević Bogoljub, Sretenović-Rajačić Tatjana

 

Agricurtural Research Institute "Srbija", Center for Vegetable Crops, Karađorđeva 71,

11420 Smederevska Palanka, cfvcsp@eunet.YU

 

The "af" gene, at its recessive homozygous stage, causes the modification of the pea leaves so that they only form vines with stipules instead of normal structure leaves. In order to investigate the effect of the "afila" genotype on the absolute seed mass of the descendants, the pea variety Filigreen of the afila type was crossed with 12 other varieties possessing normal leaf structure.

The genetic analysis of F1 and F2 generations (the segregation ratio between the normal and afila leaf type being 3:1) proved the monogenic character of this feature. The absolute seed mass in F1 generation, which was characterised by normal leaf structure, was larger in the parents of both pea types.

In F2 generation that possessed normal leaf structure, the absolute seed mass was 2% higher on the average than the one in parents, while it was 6% lower as compared to the F1 generation. The average absolute seed mass in afila pea plants was 5% lower than it was in their parents, and when compared to the F1 generation, the absolute seed mass was 12% lower. The decrease of the absolute seed mass in F2 generation of the afila genotype was conditioned by the reduction of the assimilation surface of the plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INFLUENCE OF ANTIOXIDANTS ON FERTILITY AND FECUNDITY OF D. melanogaster

 

Jelena Živanov-Čurlis, Stevo Najman, Estera Mrčarica

 

Department of biology with human genetics, Medical faculty Nis

 

Because of importance of effects of free radicals on many living functions we used the preparation Protectin (Zdravlje Leskovac) which consist of several antioxidants in order estimate how much they can affect fertility and fecundity of D. melanogaster. In this purpose we used two lines of flies. Experimental (E) line is grown on medium having Protectin, and line is grown on standard medium served as a control (C). Protectin consists of antioxidants as alpha-tocopheryl acetate, vitamin C, beta-carotene and selenium. Parental generation was grown on medium with antioxidants also. In E line was 1,75% sterile males, and sterile females were not found in estimated pairs. In C line was found 1.75% sterile males and 2,63% sterile females. Average fecundity was 36.56 hatched flies per female in C line, and 58,22 hatched flies per female in E line. Also, it's found many unusual tiny flies in E line offspring. According to our results antioxidans from Protectin reduces sterility and increases fecundity in D. melanogaster.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY AND CORRELATION ANALYSIS OF FRUIT CHARACTERISTIC OF SELECTED VINEYARD PEACH (Prunus persica L. Batsch.)

 

Gordan Zec, Radmila Todorović. Petar Mišić. Slavica Čolić

"PKB INI AGROEKONOMIK" Fruit and Grape Research Station Padinska Skela, Beograd

 

Native populations of vineyard peach in Serbia represent a rich source of genetic variability which can contribute to the improvement of economically the most important characteristics of nowadays growing varieties and rootstock of peach. Vineyard peach seedlings are. by Misic and coll. (1988.) the most important rootstock for peach in our country. By statements of Scorca et. al. (1984.), genetic variability of the newest peach varieties in the world is extremely limited.

During 1994. and 1995.. it was investigated 12 positive genotypes of vineyard peach selected from several populations in Serbia. In this work fruit characteristics of investigated selections, are presented. Among investigated selection, fruits of round and oval shape are dominant. It is represented several groups with different peel color, mesocarp color, as well as with different leaf color before falling. From total of 12 genotypes, 6 are without additional peal color and without red coloring of mesocarp around stone, while the other 6 has the additional color and red coloring represented in a different level.

On the basis of calculated coefficient of rank correlation it was obtained statistically very important correlation between basic fruit peel color and fruit mesocarp color, between the additional peel color and mesocarp color around stone, as well as between mesocarp color around stone and leaf color before falling.

Coefficient of fruit weight variation was 15,4%, while coefficient of stone weight was 10,7%. Average seed germination ranged from 68% to 108%. The results of coefficient of rank correlation confirm the former proved correlation between pigments presence in leaf and mesocarp of peach fruit (Ackerman and Hough, 1950.). Diversity of investigated fruit and leaf characteristics of vineyard peach point to distinct variability of selected genotypes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CORRELATION ANALYSIS OF SOME QUANTITATIVE TRAITS OF AUTOCHTONOUS CABBAGE POPULATIONS (Brassica oleracea var. capitata L.)

 

Mirjana Ivančević, Tatjana Sretenović-Rajičić, Nenad Pavlović, Jasmina Zdravković

 

Agricultural research Institute "Srbija", Centre for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, cfvcsp@eunet.yu

 

In order to determine the initial material for breeding aimed at obtaining favourable agronomic features, we investigated nine local populations and one cabbage variety. The trial was set up on an experimental plot of the Centre for Vegetable Crops in Smed. Palanka by applying the method of random block system in five replications during the years 1997 and 1998. Interdependence of the traits (rosette diameter, number of leaves per rosette, plant mass, head diameter, head height, core length , stem length , and head mass) was determined by coefficient of correlation (Hadzivukovic, 1991). Coefficients of correlation demonstrated a high positive correlation ratio between the traits head mass -plant mass (r = 0.769). The lowest positive correlation ratio was found for the traits rosette diameter - core length (r = 0.142). The trait number of leaves per rosette was in negative correlation with the head diameter (r = - 0.309) and the head height (r = - 0.280).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ATYPICAL PHENOMENA IN WALNUT ORGANOGENESIS

 

Janković Dragan, Janković Slađana

 

Faculty of Agriculture, Pristina

 

The knowledge of growth and development of individual organs and fruit tree as a whole is a major prerequisite for defining and proper application of cultural practices in fruit growing. In ecophysiological terms, walnut has marked specificities which are shown through organogenesis cycle, which distinguishes it from the majority of other fruit species. The proper understanding of these aspects in walnut growth and development is aggravated due to the complexity of organogenesis, pronounced proneness to cross-fertilization, heterozygosity and prevailing generative mode of propagation.

The evaluation and clarification of atypical phenomena in walnut organogenesis, which in some cases occur on individual trees every year, and in others sporadically, often induced by ecological factors, enables a better insight into the available gene fund in a certain population and a better understanding of mechanisms of expression of genes controlling walnut growth and development.

Over several years of investigation of domestic walnut population, the following atypical phenomena were recorded: late flowering, formation of racemose inflorescences containing up to 20 female flowers, formation of hermaphrodite inflorescences with fertile female flowers at the base of the inflorescence axis and male flowers near its apex, differentiation of various types of bearing branches from summer buds, formation of male bearing branches on which all the buds are differentiated into catkins (including terminal), development of catkins with a V-shaped axis, development of leaves with coalesced and deformed leaf blades, formation of fruit with three-part, four-part and five-part shell suture and corresponding kernel segmentation.

Many of these phenomena are associated with high cropping potential, hence detection and fixation of genes controlling them offer new possibilities for increasing both theoretical and real walnut yields.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GROWTH HABIT FORMS IN P. communis L. POPULATION IN THE IBAR - KOLASIN REGION

 

Slađana Janković

 

Faculty of Agriculture, Pristina

 

            Studies were carried out in 1995-1998 on differing growth habit forms in wild pear population (P. communis L.) in the region of the Ibar Kolašin. This spontaneous population, derived by generative propagation, consists of several thousands of bearing trees aged 20-200 years and is highly heterogenous.

The evaluation of growth habit was carried out through monitoring the following parameters:

- tree vigour:

- presence and development of the central leader;

- number and development of primary scaffold branches;

- brandling angles of primary scaffold branches;

- overgrowing of primary scaffold branches by the branches of the higher order;

- characteristics of overgrowing branches.

 

A marked variability in all the mentioned parameters was observed within the evaluated population. Differing forms of growth habit have been determined and classified into main types and subtypes. The paper presents a detailed schematic presentation of selected growth habit forms, as well as corresponding photodocumentation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POPULATION GENETICS INVESTIGATIONS OF PTC TASTING SENSIBILITY FROM SOME LOCALITIES IN SERBIA

 

Krajinčanić1, B., Krajinčanić-Suzović V.2, Cukić R.3, Ninković D.3

 

1 University of Belgrade

2 Health Centre Zemun Department of Mental Health

3 Faculty of Defectology, University of Belgrade

 

In our investigation of somatic and genetic characteristics of children and youth in some localities of Serbia, which are geographical}' far from one another and different according to the degree and origin of the population, we investigated PTC tasting sensibility.

The investigation included 2268 children population from East. West, North West and North part of Serbia from 7-14 years old.

Mean taste threshold for PTC in Eastern Serbia (Laznica and Zagubica) is 6.04. Western (Zlatibor - Cajetina) 5.68, West river Morava (Velika Drenova) 7.58, North Western(Novi Pazar) 6,13 and Sjenica 6,77 and Northern (Surdulica) 6.95.

Comparing the data we have found out that our results are in accordance with the other authors in our country and according the frequency of recesive phenotype "non taster" and recessive alelogen (pt) certain difference exists.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

FREQUENCY OF HAND CLASPING FROM SOME LOCALITIES IN SERBIA

 

Krajinčanić-Suzović V.1, Krajinčanić B.2, Čukić R.3, Gašić-Marušić R.4


1 Health Centre Zemun Department of Mental Health

2 University of Belgrade
3 Faculty of Defectology University of Belgrade

4 Military Academy Belgrade

 

Within doing of children and young people some localities of Serbia, the whole series of genetic markers were studied beginning with polygenic height and growth structure trough colour and hair quality, eye colour the presence of Darwin's tubercle and state of lobule in concha, hand relief-dermatoglyphics up to very strong monogenic markers such as blood group systems ABO, Rh( D ) and MN, then PTC and finger overlapping.

In this paper we present results of finger overlapping from children 7-14 years old. These characteristics inherited dominant when left finger is over right and recessive when right is over left. Comparing the data we have found out that frequency of left and right type is around 50%, and in there's no greater differences in distribution between man and woman and the investigated population.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC DIFFERENTIATION IN POPULATIONS OF Merodon avidus (ROSSI, 179) (DIPTERA: SYRPHIDAE)

 

Milankov V., Vujić A.. Simić S., Ludoški J.

 

University of Novi Sad, Institute of Biology Trg Dositeja Obradovica 2. 21000 Novi Sad, Yugoslavia

 

Natural populations of M. avidus from Mountain Dubasnica (Serbia), Morinj (Montenegro), Mountain Durniitor (Montenegro), Mavrovo lake (Macedonia) and Pindos Mountain (Greece) were assayed. Isozyme variability of twelve loci (B.C. number and locus): aldehyde oxidase (1.2.3.1; Ao), mmarate liydratase (4.2.1.2; Fum), glucosephosphate isomerase (5.3.1.9; Gpi), hexokinase (2.7.1.1; Hk), malic enzyme 1.1.1.40.; Me), phosphoglucomutase (2.7.5.1; Pgm) and superoxide dismutase (1.15.1.1.; Sod) was studied in Tris-Boric-EDTA buffer system, while aspartate amino transferases (2.6.1.1.; Aat), α-glycerophosphate dehydrogenase (1.1.1.8; Gpd-1, Gpd-2), (β-hydroxybutirate dehydrogenase (3.1.1.31; Hbd), and isocitrate dehydrogenase (1.1.1.42; Idh-2) in Tris-Citric buffer system, using the method of polyacrylamide gel electrophoresis (PAGE) according to Munstermann (1979) with a few modifications (Milankov el aL 1998).

Genetic differentiation between populations of M. avidus was evaluated on the basis of allele frequencies.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EVOLUTIONARY RELATIONSHIPS AMONG THE MEMBERS OF THE AENEUS GROUP SPECIES OF GENUS MERODON (DIPTERA: SYRPHIDAE)

 

Milankov V., Vujić A., Simić S.

 

University of Novi Sad, Institute of Biology, Trg Dositeja Obradovića 2, 21000 Novi Sad, Jugoslavija

 

The genus Merodon is the second-largest European genus of family Syrphidae. More than 50 species are known on the continent, predominantly in southern and Mediterranean part. This paper deals with one monophiletic species group that includes: Merodon aeneus complex Meigen, 1822: M. cinereus (Fabricius, 1794); M. Desuturinu,s Vujic, Simic et Radenkovic, 1995, and M. funestus (Fabricius, 1794).

Natural populations of M. aeneus (Morinj, Mountain Kopaonik, Mountain Dunnitor), M. cinereus (Mountain Kopaonik, Mountain Durmitor, Mountain Sar planina. Mountain Prokletije), M. desuturinus (Mountain Kopaonik), M. funestus (Morinj) and M. sp (Morinj, Mountain Dunnitor) were assayed. Isozyme variability of fumarate hydratase (E.G. 4.2.1.2.; Fum). glucosephosphate isomerase (E.G. 5.3.1.9.; Gpi), hexokinase (E.G. 2.7.1.1.; Hk), malic enzyme (E.G. 1.1.1.40.; Me), phosphoglucomutase (E.G. 2.7.5.1.; Pgm) and superoxide dismutasc (E.G. 1.15.1.1.; Sod) was studied in Tris-Boric-EDTA buffer system, while aspartate amino transferases (E.G. 2.6.1.1.; Aat). α-glycerophosphate dehydrogenase (E.G. 1.1.1.8.; Gpd-1, Gpd-2), β-hydroxybutirate dehydrogenase (E.G. 3.1.1.31.; Hbd). isocitrate dehydrogenase (E.G. 1.1.1.42.; Idh-1, Idh-2) and malate dehydrogenase (E.G. 1.1.1.37. Mdh) in Tris-Citric buffer system using the method of polyacrylamide gel electrophoresis (PAGE) according to Munstermann (1979), Pasteour et al., 1988, with a few modifications (Milankov et al., 1998).

Evolutionary relationships among the members of the aeneus group species was evaluated on the basis of allele frequency, as well as occurrence of species-specific allozymes at diagnostic loci.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF FREQUENCY OF SCEs AND PRI IN PERIPHERAL BLOOD LYMPHOCYTES IN CONTROL SAMPLE OF YUGOSLAV POPULATION

 

Olivera Milošević1, Dragoslav Marinković2, Slobodan Arsenijević3

 

1 Faculty of Science, Kragujevac

2 Faculty of Biology, Belgrade

3 Clinic of Gynecology, Kragujevac

 

In the present study, we represented baseline frequencies of sister-chromatid exchange (SCE) and prolipheration index (PRI), depending on sex and age of individuals. It was shown distribution of SCEs and PRIs between individuals, and also, between groups of individuals, as well as correlation between these two phenotypical features. Analyzed sample included 46 phenotypically healthy persons. Total average frequencies of SCE-s and PRI were 6.97±0.10 and 1.77±0.07, respectively. In group of female individuals with average age of 35 years, average SCE frequency was 7.33+0.10 SCE per person, that is statistically significantly higher (p<0.001) in comparison with sample of male persons with average age of 29 years (6.57±0.1 SCE per person). In sample of female persons average PRI was lower (1.70±0.()8) as compared with male persons (1.87±0.07), but without statistical significant. In order to establish effect of age mid sex on SCE and PRI frequencies, analyzed sample was allocated in three groups. In sample of persons with age from 40-63 years, SCE frequencies were significantly lower (p<0.001). Significant differences in PRI noted between age groups (p<0.001). SCE and PRI showed negative correlation (r=-0.01).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INFLUENCE OF ANTIOXIDANTS ON PREADULT DEVELOPMENTAL DURATION OF D. melanogaster

 

Stevo Najman, Jelena Živanov-Čurlis, Estera Mrčarica

Department of biology with human genetics, Medical faculty Niš

 

Many researches indicate the role of free radicals in different phases of ontogenic development, embryonic as well as aging. Drosophila melanogaster is frequent model for these researches. Using preparation Protectin (Zdravlje, Leskovac) with antioxdative vitamins we wonted to see how much antioxidants from food can modulate preadult developmental duration of D. melanogaster. In this purpose two lines of flies are investigated. Control line (C) was grown on standard medium, and experimental one (E) was on medium having Protectin, which consists of antioxidants as alpha-tocopheryl acetate, vitamin C, beta-carotene and selenium. E line is formed from flies that parental generation was grown on medium with antioxidants. First lan as were apeared third day since laying eggs in C line, but fifth day in E line. First pupas were apeared seventh day in C line, but nineth day in E line. Average duration of preadulted development in C line was 14,54 days, and in E line was 16,67 days. Differences in duration of preadult development between these two lines are statistic significant. We may conclude mat antioxidants in medium in given concentrations prolong preadult development of D. melanogaster.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ALLOZYME VARIABILITY IN Quercus robur L. AND IDENTIFICATION

POSSIBILITY WITHIN THE POPULATION

 

Dragana Obreht1, Saša Orlović2, Joška Erdesi3, Ljiljana Vapa1

 

1 Institute of Biology, Trg D. Obradovica 2, 2 1000 Novi Sad

2 Poplar Research Institute, Antona Cehova 13, 21000 Novi Sad

3 Forest Enterprises Srbijasume, Forest District Sremska Mitrovica, 22000 Sr. Mitrovica

 

During 90's a broad EU supported project concerning diversity of nuclear and cvtoplasmic gene-markers in oaks was established. The obtained results showed that populations were poorly differentiated for nuclear but highly differentiated for cytoplasmic markers. Despite the fact that polymorphism of isozymes in genus Quercus is usually low, an isozyme loci variability analysis in Common oak (Quercus robur L.) from Yugoslavia was performed because it has an economic importance in our country, and there is no such information from this European region. Seventy-six common oak trees (seed orchard, fifteen years old) were analyzed originated from locality "Banov brod", near Sremska Mitrovica. The isozymes were extracted from dormant buds, and ten isozyme loci were analyzed based on standard gel electrophoresis (starch and polyacrilamide). Four loci were polymorphic with different number of presented allelic forms, and they control the expression of enzymes with low substrate specificity (EST. PX. and ACP). The variability of chosen gene-marker system didn't allow identification of genotypes within the population.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

DISTRIBUTION OF THE KNOWN DIPLOID CHROMOSOME NUMBERS OF SOME EUTHERIAN GENERA PRESENTED IN THE FAUNAS OF CONTINENTS AND OCEANS

 

Boro P. Pavlović i Nevenka Pavlović

 

Institute of Forestry, Kneza Viseslava 3, Belgrade

Faculty of Science, Mladena Stojanovica 2, Banja Luka

 

The data of Eutherian diploid chromosome numbers (2n) have been connected with genera and with faunistic presentation of the genus. The analysis has accomplished by geographic entirety: North America, West India, South America, Madagascar, Africa, Europe, Arctic, Asia, Southeast Asia, Philippine, New Guinea, Australia, Antarctic, Indian Ocean and Pacific. Pattern of 2n distribution is specific for nearly each analyzed area. There is some regularity in grouping of the areas on the base of maximal frequencies, averages or variations of 2n, related to the origin of fauna and to clime conditions of the area.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ECOLOGICAL STABILITY OF YIELD COMPONENTS IN WHEAT

 

Petrović Sofija1, Dimitrijević Miodrag1, Mladenov Novica2 i Kraljević-Balalić Marija1

 

1 Institute for Field and Vegetable Crops, Faculty of Agriculture, Novi Sad

2 Institute of Field and Vegetable Crops, Novi Sad

 

Ecological stability of ten diverse wheat genotypes, in four years, was studied. Traits under the observation were the number of grains per spike, as well as, grain weight per spike. The highest average value for the grain number per spike exhibited variety Martonvasarska 15 (= 58.1) in 1991/92.. in a contrast to variety Balkan ( = 30.5) showing the lowest mid-value in 1994-95. Mean values of grain weight per spike ranged from  = 1.1g, for variety Balkan in 1994/95., to  = 2.8g, for variety NSR-2 in 1991/92 year. Eberhart and Russell (1966) model was used for stability studies. The most stable varieties were Libelulla (bi= 1.364 and Sdi2= 1.688) and Lovrin 21 (bi = 1.005 and Sdi2=1.656) considering grain number per spike. The best stability performance for grain weight per spike appeared for varieties NSR-2 (bi = 1.041 and Sdi2= 0.289), as well as, Yugoslavia (bi = 0.862 and Sdi2= 0.155).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MONITORING OF TEMPORAL VARIABILITY OF CHROMOSOMAL ARANGEMENTS IN Drosophila subobscura

 

Vuk Savković2, Marko Anđelković2,1

 

1 Institute for biological research "S. Stankovic", Belgrade

2 Faculty of Biology. University of Belgrade

 

Time distribution monitoring of individuals within a certain habitat allows an insight into correlation between certain types of genetic polymorphism and specific ecological factors, which inevitably vary along with the time of day.

Inversion polymorphism in some Drosophila species demonstrates a degree of flexibility regarding a reaction to changes of ecological factors. There is an abundance of data related to rich inversion polymorphism in Drosophila subobscura, enabling different judgments on the correlation mentioned.

Bearing that in mind, we have carried out an inversion polymorphism analysis of D. subobscura individuals captured at different times of day at beech forest habitat at the Goc mountain. The time of capturing has been adjusted according to the differences of circadial activity in this species.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

REACTIVITY OF CABBAGE (Brassica oleracea var. capitata) ON PARAQUAT IN VITRO

 

Tatjana Sretenović Rajičić, Mirjana Ivančević, Jasmina Zdravković, R. Đorđević, M. Damjanović

 

Agricultural Research Institute "Serbia", Centre for Vegetable Crops, Smederevska Palanka

 

The aim of our study was to investigate genetic component of cabbage (Brassica oleracea var. capitata) reactivity on total herbicide paraquat in vitro. We used shoot culture of few cabbage genotypes as a model system suitable for this experiment. Examined parameters were root number, root length, shoot length, fresh and dry mass. We established analysis of variance for these parameters, wide sense heritability (h2), coefficient of phenotype variation (CVf), correlation and regression analysis.

Heritability was very high at all parameters, especially at fresh and dry mass (h2=92%). The most variable parameters are number of roots (CVf=29.36) and root length (CVf=26.57). Root number, root and shoot length are significantly correlated. At P295 and P6p genotypes, there is almost physiological dependence between these parameters (b=0.99**). Regression coefficients of dry on fresh mass are very high at all examined genotypes (from bp295=12.60 to bP6p= 17.39).

Changes induced by paraquat are highly determined by genetic component of variance, which is indicated by coefficient of heritability. Paraquat reduced all examined parameters. Similarity in profile of reaction on paraquat and very strong correlation between growth parameters indicates that these parameters are determined by structural polygenes and that these are constitutive characteristics. Differences in intensity of changes that paraquat induces in growth of shoot culture indicate that there are some differences between examined genotypes in regulatory polygenes that determine expression of these changes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BIODIVERSITY OF THE HONEYBEE Apis mellifera, Linne (1758) - CYTOGENETIC ASPECTS

 

Z. Stanimirović, Jevrosima Stevanović, D. Pejović, D. Popesković

 

Faculty of veterinary medicine, Belgrade

 

This paper presents the results of the comparative biometric studies of the chromosomes and comparative ultrastructural chromosomal analyses (the distribution of euchromatin and heterochromatin) of the two studied indigenous honeybee ecotypes (the Banate - BET ecotype and Sjenichko - Peshtersky - SET ecotype). These ecotypes originate from ecogeographically defined Yugoslav regions that differ significantly in many microgeografic and microclimatic elements.

The chromosomes for the cytogenetic analysis were obtained from nervous tissue of the cerebral ganglia of honeybee praepupaes according to the procedure established by Imai et al. (1988). G - banding of chromosomes was done by the method of Ronne (1991), which in itself presents a modification and an improvement of those established by Seabright (1971) and Verma (1998).

The conducted biometric analyses point to the existence of differences in those values pertaining to the relative chromosome length and centromere index (arm ratio). The greatest differences in the relative lengths of chromosomes were observed between the chromosomes 12, 2, 3, 1 and 6 in favour of the SET ecotype, namely 15, 14 and 11 chromosomes in favour of the BET referential ecotype. However, the monitoring of the centromere index revealed the greatest differences between the chromosomes 16, 1, 2 and 4. On the basis of these results we advanced the hypothesis that the observed chromosomal biometric differences are the result of the amplification and rearrangement of chromosome regions of the representatives of the studied honeybee ecotypes. This is what prompted us to undertake further investigations which included ultrastructural chromosome analyses. These analyses point to the existence of a marked difference in the G - band distribution on the chromosomes 1, 2, 4, 11, 12, 13, 15 and 16 of the SET honeybee ecotype with relation to the BET ecotype, thus confirming our previously advanced hypothesis.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE INVESTIGATIONS OF CHROMOSOME POLYMORPHYSM IN THE NATURAL POPULATIONS OF THE SPECIES Mus musculus, Linne (1758), ON THE TERRITORY OF THE EX YUGOSLAVIA

 

Z. Stanimirović, B. Soldatović, Jevrosima Stevanović

 

Faculty of veterinary medicine, Belgrade

 

The study of chromosome polymorphysm in the natural populations of the species Mus musculus, Linne (1758), was conducted at 45 sites on the territory of the ex Yugoslavia, south of the rivers Sava and Danube. The examined corpus included 458 trapped and sacrificed animals (231 males and 227 females). At the industrially more developed localities, where the degree of the environmental contamination was much higher, the trapping of the animals had to be repeated several times.

The chromosomes intended for the karyotype analysis were obtained by the direct puncture of bone marrow from long tubular bones (the femur and humerus) according to the method of Hsu and Pat on (1969), modified by Zimonjic (1990), and by the lymphocyte cell culture from the peripheral blood in vitro according to the method of Evans and O'Riordan (1996, 1997). In order to obtain prometaphasal chromosomes the synchronization with bromdeoxyuridine was performed according to the procedure of Dutrillaux and Viegas-Pequignot (1981). G-banding was done according to the method of S e a b r i g ht (1971) and Y un i s (1978), modified by R o n n e (1991).

The identification of chromosomes and chromosome bands was done on the basis of the criteria established by the Committee on Standardized Genetic Nomenclature for mice (1972, 1979) and the Cowell's photo atlas of the house mouse chromosomes.

The obtained results proved the existence of chromosome polymorphysm of Robertsonian type in the natural populations of the species Mus musculus not only in Dalmatia, Montenegro and southeastern Macedonia, as known from the existing literature (Bulic and Soldatovic. 1980; Tichy at al. 1987; Winking et al. 1988) but also in the central (bosnia and Herzegovina) and eastern parts of the ex-Yugoslavia (Serbia). At the analyzed sites in Serbia 60. 20% of the examined animals were either homozygous for the chromosomes with Robertsonian translocations; in Bosnia and Herzegovina it appeared to 59,45% and in Macedonia to 52,05% of the examined animals.

With the identification of the Robertsonian chromosomes we ascertained that, in addition to the "mutual" Rb. 5/15 chromosome already described in literature relevant for the natural populations of the species Mus musculus from Europe and North Africa (Giagia et al. 1982; Tichy at al. 1988), the mice of the natural populations from the sites in Serbia contained in their karyotype a so far unknown, new form of the Robertsonian chromosome, formed by fusion of the 4th and 19th autosomes (Rb. 4/19). In the karyotype of the animals originating from the localities in Maclwa and in the vicinity of Sremska Mitrovica was noticed the presence of a larger acrocentric chromosome, from the first pair of autosomes where, by applying the G-technique. 2 insertions were observed {1C 5 and IE (E3andE4)}.

Having in mind the fact that at the monitored localities m Yugoslavia both animals with Robertsonian translocations and mice with standard karyotype were trapped, it can be concluded that original populations of the species Mus musculus belonged to the subspecies Mus musculus musculus, and that the with the passing time, due to an introduction and migration of animals, the Robertsonian chromosomes from other populations of the "western system" (subspecies Mus musculus domesticus, Mus musculus bervirostrus, Mus musculus praetextial) were introduced into and spread among the new populations. The centres of the spreading of Robertsonian polymorphysm were, most probably, along the Adriatic coast and in southwestern Macedonia, from where animal, the carriers of the Robertsonian chromosomes, migrated towards the central parts of the ex-Yugoslavia, otherwise inhabited by the populations of Mus musculus musculus, whose representatives had the karyotype with 2n = 40 chromosomes, i.e. were monomorphic.

 

Return to content

 



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE EFFECT OF TRAUMA ON THE HAPTOGLOBIN, α2- MACROGLOBULIN, IL-1, IL-6 I TNF CONCENTRATIONS IN AO AND DA INBRED RATS

 

Milica Strnać1, Zvonko Magić2, Cedomir Radojičić2 i Dragoslav Marinković3

 

1 Institut of Patology, MMA, Belgrade   

2 Institut for Medical Research. MM A, Belgrade

3 Faculty of Biology, University of Belgrade

 

The response of on organism to the trauma is polygenically determined and probably some genes or a group of genes are more responsible than others for the resistance to trauma. Reaction of an organism to trauma starts with acute-phase reaction (APR) that consists of the local and systemic reaction. Increased synthesis acute-phase proteins (APP) in the liver represent the most prominent aspect of APR. This is the heterogenous group of plasma proteins which helps an injured organism to restore homeostatic balance disturbed by traumatic agents. Haptoglobin (Hp) and α2-macroglobulin (α2-M) are among the most important APP rats. In this study AO (Albino Oxford) and DA (Dark Auguslin) inbred strains of rats, with different resistance to trauma, were used to compare concentrations of Hp, α2-M, IL-1, IL-6 and TNF, before and after tournique trauma. Cytokines IL-1. IL-6 and TNF are the most important inducers of APP gene expression. Our results showed that before trauma, more resistant AO rats had higher concentrations of Hp and α2-M compared to DA rats, while their concentrations in the early period after trauma were lower. The influence of genetic elements of these differences is significant. Tourniquets trauma caused early increase in the serum IL-1, IL-6 and TNF activities, which preceded increase in the Hp and α2-M concentrations but strain differences, were not found. On the basis of these results we concluded that higher basal concentrations of Hp and α2-M in the AO rats and their effect in the early period of trauma are important elements of their better response to trauma.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC VARIABILITY OF MORPHOMETRICAL TRAITS IN A D. subobscura POPULATION ACROSS DIFFERENT DEVELOPMENTAL TEMPERATURES

 

IvanaTomišić1, Marina Stamenković-Radak 2,1, Tatjana Terzić1 i Marko Anđelković2,1

 

1 Institute for Biological Research, Belgrade

2 Faculty of Biology, University of Belgrade

 

Populations of various species may be exposed to temperature changes in the environment. Populations may respond to such variations at the phenotypic and genetic levels. The array of phenotypes that develop in response to several environments is the reaction norm which is observed through the concept of genotype x environment interactions.

Reaction norms across three developmental temperatures (16°C, 19°C and 23°C) were measured for wing length, thorax length and wing/thorax length ratio, in both sexes in isofemale lines from D. subobscura population.

The adaptive significance of the observed variability in different environments is discussed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MICRONUCLEUS ASSAY IN TO HUMAN BIOMONITORING

 

Gordana Joksić, Miroslava Stanković

 

"Vinca", Institute of Nuclear Sciences, Belgrade, POB 522, Medical Protection Center

 

Biological markers are divided into three major categories: exposure, effect and susceptibility. Genetic monitoring is mainly concerned with markers of exposure and effect, while genetic screening is concerned with susceptibility. Peripheral lymphocytes are the cell population of choice for most genetic monitoring studies because they are readily obtainable from a blood sample. One of the major advantages in using circulating lymphocytes is their contact with many body tissues, thus providing a more integrated measure of biological response of exposure.

In this paper we present the results of micronucleus test performed in three groups of persons occupationally exposed to low doses of ionizing radiation. Micronuclei analysis considers 43 persons handling unsealed sources of ionizing radaition and 40 controls. Values recorded for the frequency of micronuclei (MN) in the group handling organically bound tritium 0.035±0.019 MN per binucleated cell, 0.021+0.029 MN per binucleated cell in the group of workers handling 201T1 and 0.17±0.6 MN per binucleated cells in the group handling 99mTc. The average incidence of micronuclei in matched controls was 0.011+0.04 MN per binucleated cell. The yield of micronuclei in exposed workers was significantly higher (p<0.05) compared with their matched controls. The yield of micronuclei in the group dealing with organically bound tritium was significantly higher (p<0.005) as compared to other two groups of exposed subjects. The results are discussed with respect to individual variations in the response to low doses of ionizing radiation, disorders in the handling of radionuclides, the duration of occupational exposure and chemical properties of the radionuclides used.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE EFFECTS OF DISTURBED ENVIRONMENT ON NATURAL POPULATIONS OF RODENTS

 

Miroslava Veličković, Jelena Blagojević i Mladen Vujošević

 

Institute for Biological Research "S. Stankovic", Belgrade

The effect which the environment, disturbed by antropogenic action, exerts on genetic structure of natural populations is possible to assess only by using combined ecogenotoxicological approach. In such investigation first of all it is necessary to establish standards for populations from unpolluted environment. Populations of rodents were chosen for such investigations due to their lugh abundance and wide distribution. We have chosen the following species due to their frequent presence at the most localities: Apodemus agrarius, A. flavicollis, A. microps and Clethrionomys glareolus. The first approach was to establish the level of spontaneous chromosome aberrations in populations of all species studied. Tins was done following the rules of the genotoxicity test which is done in vivo on laboratory mouse. The second approach was based on the morphometric analyses of discrete cranial characters (foramina). There are a lot of data showing that environment pollution can disrupt developmental stability. Fluctuating asymmetry (FA) is accepted measure for estimating the level of developmental stability. The results obtained in populations from unpolluted environment, both chromosome aberrations and level of FA, were statistically analysed against the same parameters for populations from disturbed environment.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COMPARATIVE STUDY OF ANTIGENOTOXIC EFFECT OF SAGE IN PROKARYOTIC AND EUKARYOTIC TEST-SYSTEMS

 

Branka Vuković-Gačić1, Tatjana Stević1, Jelena Knežević-Vukčević1, Dragana Mitić1, Dejan Brkić2, Jelena Blagojević3, Marko Anđelković3, Draga Simić1

 

1Laboratory for Microbiology, Faculty of Biology. University of Belgrade

2 Institute for Medicinal Plant Research "Dr Josif Pancic"

3 Department of Genetics, Institute for Biological Research "Sinisa Stankovic", Belgrade

 

Detection of inhibitors and modulators of genotoxic agents and elucidation of the mechanisms involved in protection in prokaryotic and eukaryotic cells are aimed at prevention of cancer. There is extensive evidence about antigenotoxic effects of medicinal and aromatic plant extracts. Based on our previous research, differently prepared extracts of sage (Salvia officinalis L.) were extensively tested. Comparative study of antigenotoxic potential was conducted with ethereal oil of sage and its fractions. The reliable battery of tests was used: Escherichia cali K12, Salmonella typhimurium (Ames test), Saccharomvces cerevisiae D7, Drosophila melanogaster SLRL test and in vivo test of induction of chromosomal aberrations in bone marrow of mice.

All sage extract tested showed inhibition of mutagenesis in bacterial short-term tests. Screening in higher eukaryotic organisms was performed with ethereal oils of sage, whose fractions showed inhibitory effect on UV-induced mutagenesis in lower eukaryote S. cerevisiae. In our experimental conditions neither genotoxic nor antigenotoxic effect of ethereal oil was recorded in D. melanogaster, but UV-induced sterility of males was significantly reduced.

Moreover, the preliminary results indicate that induction of chromosomal aberration by mitomycin C in mice bone marrow in vivo was inhibited by ethereal oil of sage.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECT OF DIFFERENT DOSES OF GESTAGENS ON MICRONUCLEUS FREQUENCY IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES

 

Olivera Milosević1, Darko Grujičić1, Dragoslav Marinković2, Slobodan Arsenijević3,

Smilja Banković3, Aleksandar Živanović3, Aleksandra Dimitrijević3

 

1 Faculty of Science, Kragujevac

2 Faculty of Biology, Belgrade

3 Clinic of Gynecology, Kragujevac

 

Since gestagens are widely used in gynecology. we decided to determine the genotoxic effect of these preparations by using CB micronucleus test. Analyzed sample consisted of 30 pregnant women. Frequency of MN was determined before and after applied therapy, as well as in 15 newborns, whose mothers received therapy during their pregnancies. Average MN frequency in patients after applied therapy (20.14±1,33 MN/1000 analyzed binuclear cells) was increased 1,5 time as compared with average MN frequency in the same patients before therapy (13,00±1,16 MN/1000), with statistical significance p<0.001. Correlation between doses and MN frequency after therapy was positive (r=0,33).

In order to establish the effect of different doses of gestagens on MN frequency, analyzed sample was allocated in to three groups. Results showed that the average MN frequency increased significantly (p<0,001), after therapeutic treatment in all three groups in comparison with average MN frequency in the same groups before therapy. The highest increase in MN frequency was found in group of patients treated with high doses of gestagens: 2000-8400mg. Average MN frequency in sample of newborns whose mothers received therapy (16,47±2.42 MN/1000) was increased 3 times as compared with MN frequency in control sample of newborns (5.27+0.59 MN/1000).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EVALUATION OF GENOTOXIC AND MITOGENIC EFFECTS IN CULTURES OF HUMAN LYMPHOCYTES TREATED WITH INSULIN

 

Ninoslav Đelić1, Bogosav Soldatović1, Marko Anđelković2

 

1 Faculty of Veterinary Medicine, University of Beograd, Bul. JNA 18, 11000 Beograd

2 Faculty of Biology, University of Beograd, Akademski Trg 16, 11000 Beograd

 

Insulin is a major anabolic hormone in mammals. Overwhelming evidence indicate that insulin increases DNA synthesis and cell cycle kinetics of various cultured cell lines [1]. Mitogenic effects of insulin may contribute to the development of cancer. Having in mind that genetic lesions underlie most of the known malignant diseases, our opinion is that genotoxicological characterization of insulin should be performed. In vitro genotoxicity of insulin by standard cytogenetic and sister-chromatid exchange techniques pointed to negative results [2]. The aim of this investigation was to evaluate possible aneugenic and/or clastogenic effects of human recombinant insulin in cytochalasine-blocked micronucleus assay. In addition, analysis of mitotic and proliferation indices were used to examine effects on cell cycle kinetics of cultured human lymphocytes.

Human recombinant insulin (Inutral® HM-100, ICN Galenika, CAS No 11061-704) was tested in seven experimental concentrations in a range from 10-10 M to 0,75x10-5 M. These concentrations correspond to physiological, therapeutical and 30 fold maximal therapeutical doses in human medicine.

On the ground of the obtained results it can be concluded that insulin exerts maximal mitogenic effects at concentration of 10-8 M. On the other hand, in vitro micronucleus test confirmed the absence of genotoxic effects of human recombinant insulin.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INFLUENCE OF CLOPROSTENOL ON CELL CYCLE KINETICS IN CULTURED HUMAN LYMPHOCYTES

 

Ninoslav Đelić, Bogosav Soldatović, Dijana Đelić

 

Faculty of Veterinary Medicine, University of Beograd, Bul. JNA 18, 11000 Beograd

 

Cloprostenol, the synthetic analogue of prostaglandin F2 alpha, is widely used in veterinary medicine (i.e. estrous synchronization in cattle). Previous experiments in our laboratory [1] revealed the absence of genotoxic effects of cloprostenol in cytogenetic tests in vitro and in vivo, whereas in SCE in vitro lest cloprostenol exhibited genotoxic potential [2]. The aim of this investigation was to evaluate possible cytotoxic or mitogenic effects of the same concentrations of cloprostenol, using the analysis of mitotic and proliferation indices [3].

At the beginning of incubation 5-bromo-2'-deoxyuridine was added to cultivation vials, whereas the staining of metaphase spreads was performed according to a slight modification of FPG technique (Perry mid Wolff. 1974).

The obtained results have not revealed significant changes in mitotic index. In addition, on the basis of proliferation index analysis it is evident that cloprostenol has not changed cell cycle kinetics of human lymphocytes in culture. Therefore, these results are in agreement with assumption that cloprostenol can not exhibit carcinogenic effects by modulation of mitotic divisions in target cells.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INVESTIGATION OF GENOTOXIC EFFECTS OF CARISOLV™ GEL

 

Biljana Burić, Z. Stanimirović, Biljana Marković


Fakultet veterinarske medicine, Beograd

 

A revolutionary drug for the treatment of caries disease Carisolv™ (a painless process without the use of a drill), provided by Medi Team from Sweden, represents a real advancement in modern stomatology. Its significant properties lead us to analyze its genotoxic potential and mutagenity.

The influence of Carisolv™ on the occurrence of structural and numerical chromosomal aberrations in vitro was examined in cultures of human peripheral blood. The lymphocyte cultures were treated with tree concentrations of Carisolv™ therapeutic dose- 1 mg and subtherapeutic doses 0.5 mg and 0,1 mg. Negative as well as positive controls were included.

The occurrence of chromatid and chromosomal breaks and gaps was evaluated among the structural aberrations, as well as the occurrence of aneuploid and polyploid cells among numerical aberrations. We used Evans and O'Riordan (1976) methods for the culturing and preparation of human lymphocytes, modified by Zimonic (1990). Cell culturing and preparation for differential imaging of sister chromatides were used according to the procedures of Perry and Evans, revised by Zimonic (1990).

Within the range of the tested concentrations, Carisolv™ did not induce significant alterations in the occurrence of structural and numerical aberrations, compared to the negative control level. At the same time a stimulating effect on the proliferative cell activity was observed at the therapeutic dose of 1 ml of Carisolv™. This dose brought about a highly significant increase in the mean value of the mitotic index, (p<0,001) compared to the control.

On the basis of the results obtained it is obvious that Carisolv™ did not produce genotoxic effects in the tested concentrations.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CHROMOSOME ABERRATIONS MONITORED IN RADIATION WORKERS

 

Gordana Joksić, Miroslava Stanković

 

"Vinca", Institute of Nuclear Sciences, Medical Protection Centre, POB 522 Belgrade

 

The health surveillance of radiation workers has been performed for many years in our country. During the 5 years. 256 persons occupationally exposed to ionising radiation were referred to our Institute for the investigation by cytogenetic analysis. Of these, 98 were associated with industrial uses of radiation, and 158 were from health institutions. Health workers considered in the survey are categorised into several major groups according to the type of application with which they are involved: radiologists (27), dark room technicians (71). nuclear medicine (26) and medical workers performing invasive methods in X-ray diagnostics (34). The average duration of occupational exposition was in the range from 9.6±4.7 to 17.8±4.5 years. All radiation workers monitored during 5 years period have used TL dosimeters for external exposure measurement. The TL dosimeters were worn on the trunk of chest level. The total annual doses to radiation workers varied between 2.6 and 33.6 mSv: the lowest was found for industrial technicians the highest for workers in invasive X-ray diagnostics. The relatively high annual average doses were recorded in persons performing invasive radiological diagnostics in cardiology (catheterisations) and urology (percutaneous drainages). No evidence of radiation exposure, as indicated by dicentric chromosomal aberrations in blood lymphocytes, was found in 196 persons: in the group of dark room and industrial technicians. In the remainder chromosomal aberration yields were mostly consistent with low doses, which is below the statistical limit of reasonable accuracy for the technique. The most serious cases investigated were among workers in invasive X-ray diagnostics where 50% were dicentric positive. Moreover in 4 of dicentric positive persons (cardiologists) few heavily damaged cells were found that is unrelated to radiation and are probably due to virus infections (human polyoma viruses), which appears to be serious addition professional risk.

No correlation between the incidence of structural chromosomal aberrations and the duration of occupational exposure was found (r=0.34, p<0.05). Statistically significant correlation was recorded between the incidence of chromosomal aberrations and source of radiation used (r=0.66, p<0.05), as well as with doses recorded on TL dosimeters (r-0.54, p<0.05).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ANALYSIS OF CHROMOSOMAL STATUS OF SUBJECTS OCCUPATIONALLY EXPOSED TO RADIONUCLIDES

 

Dubravka Jovičić1, Snežana Milačić1, Radomir Kovačević1, Mitar Novaković2

 

1 Clinical Centar of Serbia, Institute of Occupational Medicine and Radiological Protection "Dr Dragomir Karajovic" Deligradska 29, Belgrade
2
School of Medicine, Department of Human Genetics, Banja Luka

 

It has been evidenced that ionizing radiation induces certain changes in chemical constituents of cells. As a result, structural changes may ensue that can affect the cellular biological functions. The results of long studies of individuals occupationally exposed to radionuclides are presented in this study. A group of professionals working in nuclear medicine (99 subjects work with open sources of radiation) are studied. Cytogenetic studies have shown a different frequency of chromosomal aberrations in certain study periods. Significant increase of the frequency was recorded in the period 1995-96 when the percentage ranged from 19. l% to 10%. The following chromosomal aberrations were noted: dicentric, ring chromosomes and acentric fragments. In 1997 professional working in nuclear medicine did not exhibit unstable chromosomal aberrations, probably because our sample was very small.

A wide spectrum of radiosotopes has been used in nuclear medicine. Therefore, care should be taken to educate the personnel appropriately as well as to provide the adequate working space for radionuclide studies, together with maximum protection of occupationally exposed subjects.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MOLECULAR MECHANISMS OF MUTAGENESIS INHIBITION BY ETHEREAL OIL OF SAGE (Salvia officinalis L.)

 

Jelena Knežević-Vukčević, Tatjana Stević, Dragana Mitić, Branka Vuković-Gačić, Draga Simić

 

Laboratory for Microbiology, Faculty of Biology, University of Belgrade

 

Mutations play a central role in fundamental biological processes such as evolution, genetic diversity, aging and carcinogenesis, so induced mutagenesis is extensively studied. A variety of chemical mutagens and carcinogens have been detected in foods, medicines, cosmetics, insecticides, and even in the atmosphere and water. The study of inhibitors and modulators of the effects of environmental genotoxic agents is aimed at understanding the mechanisms involved in the protection against mutagens and carcinogens.

It is shown that active substances from medicinal and aromatics plants posses antimutagenic and anticancerogenic activity. Our work is designed to detect bioantimulagens, agents that prevent mutagenesis by modulating DNA repair and replication, in ethereal oil of sage. We used specially constructed E. coli K12 assay system for the detection of bioantimutagens and their possible mechanism(s) of actions (Simi) et al.. 1997: 1998). To exclude desmutagens factors that act directly on mutagens or their precursors and inactivate them, we used UV-irradiation as a mutagen.

Experiments have shown that ethereal oil of sage inhibits both spontaneous (25%) and UV-induced mutagenesis (53%) by inhibiting SOS induction and stimulating recombination.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COMPARATIVE ANALYSIS OF GENOTOXIC EFFECTS OF GASTROGAL 10 TIAMUL1N S AND CARBADOX IN VITRO

 

Biljana Marković, Z. Stanimirović, Jevrosima Stevanović, N. Jovanović

 

Faculty of veterinary medicine, Belgrade

 

The genotoxic effect of the antibiotic preparations Carbadox. Gastrogal 10 and Tiamulin S was examined, as well as their ability to induce numerical and structural chromosomal aberration in cultures of pig peripheral blood. The antibiotics were tested at the therapeutic doses (Carbadox - 40 μg/ml, Tiamulin S - 100 μg/ml and Gaslrogal 10 - 100 μg/ml). In addition, we tested the doses which are 25% and 50% less than the therapeutic one (Carbadox - 10 (μg/ml and 20 μg/ml, Tiamulin S - 25 μg/ml and 50 μg/ml. Gastrogal 10 - 25 μg/ml and 50 μg/ml). Each experimental cycle included a control culture. The procedure concerning the treatment of the media and preparations for differential staining of sister chromauds for analysis sister-chromatid exchange frequencies, was done in accordance with the technique of Perry and Evans (1975), with slight modifications by Zimonjic et al. (1990). In vitro analyses of the cytogenetic effects of the antibiotic preparations Carbadox, that Gastrogal 10 and Tiamulin S indicated each tested dose induced transformations of the karyotype of pigs lymphocytes - numerical chromosomal changes (aneuploidy, polyploidy) and structural chromosomal changes of the lesion and break type.

The total number of cytogenetic changes in pig lymphocytes, induced by the tested pharmaceuticals, was statistically highly significant in comparison with the control. The same significance was noticed after a mutual comparison of the cultures treated in vitro with different doses of the same preparation, which pointed to different intensity of the genotoxic potential, as well as to the correlation between the dose increase and their ability to induce cytogenetic changes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ANTIMUTAGENIC POTENTIAL OF ROSMANOL-9-ETHYL ETHER, OSAINE AND POMIFERINE

 

Dragana Mitić1, Tanja Berić1, Branka Vuković-Gačić1, Jelena Knežević-Vukčević1, Ratko M. Jankov2, Draga Simić1

 

1 Laboratory for Microbiology, Faculty of Biology, University of Belgrade

2 Laboratory for Biochemistry, Faculty of Chemistry, University of Belgrade

 

The study of active substances from medicinal and aromatic plants has revealed that the variety of compounds, such as vitamins, phenolic compounds, flavonoids, terpenoids. etc., posses antio.xidative activity. These natural antioxidants and their metabolites can modulate the mutagenesis process, inhibit the initiation step in carcinogenesis and interfere with tumor promotion and progression. In this work we tested antimutagenic potential of terpenoid fractions of cultivated and wild sage (Salvia officinalis L.) with different proportion of rosmanol-9-ethyl ether, one of the most active natural antioxidants. In addition, two compounds purified from osage orange fruits (M. pomifera Rob.), pomiferine and osaine, having similar structure but differing in their antioxidative potential were tested. Antimutagenic potential due to antioxidative properties was screened using two prokaryolic test systems: Salmonella typhimurium TA98 strain, hisD3052rfaΔ (uvrB-bio)/pKM101, for detection of induced hisD3052→His+ reversions and Escherichia coli K12 IB106 strain, argE3 mutT::Tn5, deficient in repair of oxidative damage of guanine nucleotide (8-oxo-G), for detection of spontaneous argE3 → Arg+ reversions. TA98 strain was treated with ethidium bromide (EtBr), with or without metabolic activation and the reduction of induced reversions was monitored. BHT was used as model antioxidant.

Antimutagenic effect against EtBr-induced mutations was observed in all fractions tested. The strongest antimutagenic effect was observed with CO: re-extract of cultivated sage E2/5, containing 40% of rosmanol-9-ethyl ether. Pomiferine is stronger inhibitor of EtBr-induced mutagenesis compared to osain, which is in good correlation with the difference in their antioxidative potential. The significant reduction of the frequency of spontaneous mutations in mutT strain was obtained only with E2/5.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COLONISATION OF WHEAT BY PSEUDOMONAS AND MARKER GENES

 

Danica Mićanović1, Penny Hirsch2, Philip Curnow2. Vera Raičević3 i Desimir Knežević1

 

1 ARI "SERBIA", Center for Small Grains. 34000 Kragujevac, Yugoslavia

2I IACR Rothamsted, AL5 2JQ Harpenden, England

3 Faculty of Agriculture, 11080 Belgrade, Yugoslavia

 

The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain Pseudomonas which containing marker genes (insert B20 with nptll gene confers resistance to both neomycin and kanamycin-colection of microbiological lab IACR Rothamsed).

Number of bacteria was obtained from bulk soil, rhizosphere/rhizoplane, rhizoplane using phase contrast microscopy. Mutants were detected using selective agar for Pseudomonas with neomycin and the genetic diversity with molecular genetic method PCR.

On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heteretroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 (using ERIC-PCR and antibiotic-resistance phenotype on selective agar) and indicates that it may be more competitive than the other two mutants.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ANALYSIS OF GENOTOXIC AND MUTAGENIC EFFECT OF APITOL®

 

D. Pejović, Z. Stanimirović, B. Soldatović

Faculty of veterinary medicine, Belgrade

 

Apitol® is a commercial preparation based on cymiazol hydrochloride (Evrotom -Ruma, Yugoslavia). It represents an ectoantiparasitic - antivarootic (praparation against Varroa jacobsoni - honeybee mite). This drug has a systemic effect on honeybee haemolymph inducing death of adult parasites. Apitol® is widely used in treating honeybee varosis and this is what prompted us to undertake some investigations of this drug.

Experimental investigations of genotoxic effects of Apitol® were performed on the human lymphocytes of the peripheral blood. The lymphocyte cultures were treated with three concentrations of Apitol8 (therapeutic dose and two subtherapeutic doses). The in vitro experiment was conducted according to the procedure of Evans and O'Riordan (1976, 1977) modified by Zimonjic et al. (1990). The procedure concerning the threatment of the media and preparations for differential staining of sister chromatis, was done in accordance with technique of Perry and Evans (1975) modified by Zimonjic (1990).

Very significant increase in the mitotic index was obtained for therapeutic dose, compared to the control. Therapeutic dose also induced significant frequency of structural chromosomal changes of the lesion and break types. Significant deviation in SCE frequency are obtained in experimental cultures (more than 13 exchange per chromosome), compared to the control (about 4 exchange), which point to genotoxic potential of Apitol.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INVESTIGATIONS OF CYTOTOXIC AND GENOTOXIC EFFECTS OF LEVAMISOLE® IN VIVO

 

Z. Stanimirović, Jevrosima Stevanović, M. Kulić, D. Pejović

 

Faculty of veterinary medicine, Belgrade

 

Levamizol is an antihelminthic drug produced by ICN-Galenika, Yugoslavia, (Catalog No.: 155228) which is widely used in veterinary medicine for roundworm infection. This is what prompted us to undertake some investigations of this drug.

The cytotoxic and genotoxic effects of levamisole hydrohloride were examined on seven weeks old male Wistar rats. These rats were divided into tliree groups. The control group was treated with saline. The experimental animals were injected intraperitoneally with two therapeutic doses of the antihelminthic (2,2 mg levamisole hydrohloride per kg b. w. and 4,4 mg levamisole hydrohloride per kg b. w.) during the seven day treatment period. The chromosomes necessary for the cytogenetic analysis were obtained by the direct puncture of bone marrow from long tubular bones, according to the method of Hsu and Paton (1969), modified by Zimonjic et al. (1990).

In order to evaluate the cytotoxic effects of the examined drug, the mitotic index was determined as well as structural and numerical chromosomal aberrations. Both tested doses of levamisole hydrochloride induced an increase in the mitotic index, numerical chromosomal changes (aneuploidy, polyploidy) and structural chromosomal changes of the lesion, break and insertion types. Insertion was observed on the first pair of autosomes between bands q21 and q22. Our results point at certain genotoxic potentials of the tested concentrations of levamisole hydrohloride.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CYTOGENETIC EFFECT OF FUMAGILLIN-ET® ON HUMAN LYMPHOCYTES IN VITRO

 

Jevrosima Stevanović, Z. Stanimirović, D. Pejović


Faculty of veterinary medicine, Belgrade

 

Fumagillin dicyklohexilamin is a crystal antibiotic, produced from the culture of one strain of Aspergillus fumigatus. Because of its wide use ir veterinary medicine (acute and chronic bee nosemosis), and in treatment of intestinal amebiasis in humans, too, the investigations of the cytogenetic effects of this drug were undertaken. The effects of the commercial preparation Fumagillin-et® (Evrotom - Ruma, Yugoslavia) on the occurrence of structural and numerical chromosomal aberrations was examined in cultures of human peripheral blood. The in vitro experiment was conducted according to the procedure of Evans and O'Riordan (1976, 1977) modified by Zimonjic et al. (1990). The lymphocyte cultures were treated with three concentrations of fumagillin (0.8 mg/ml, 0.4 mg/ml and 0.08 mg/ml). Each tested dose of Fumagillin-et® induced numerical chromosomal changes (aneuploidy) and structural chromosomal changes of the lesion and break types.

The cytogenetic study of metaphase spreads of PHA-stimulated human lymphocytes also involved the determination of the mitotic index for each experimental concentration and control. The obtained results showed that tested concentrations of Fumagillin-et® induce antiproliferative effect that is manifested with a decrease in the mitotic index, compared to the control.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BREEDING POTENTIAL OF EARLY MATURITY LOCAL MAIZE POPULATIONS

 

Gordana Radović, Dražen Jelovac, Jasmina Muminović

 

Maize Research Institute, Zemun Polje

 

A study of local maize genotypes of a shorter growing season is primarily important for a development of initial breeding material, for deriving hybrids for the northern maize growing regions, hybrids grown at higher altitudes, as well as for hybrids grown as a second crop in the Com Belt regions.

In addition to early maturity, properties of this material, that are generally important for breeding, are notable early growth, specific plant structure, shorter plant with lower number of leaves thus enabling greater crop density, resistance to frost, reduced soil fertility requirements of crop, etc.

Approximately 40% of local maize genotypes of the national maize collection, conserved at the Genebank for Maize of the Maize Research institute Zemun Polje, belong to early and medium early maturity populations of flint and semi-flint kernel type. This material is genetically very diverse, as it was derived from several successive introductions of original genotypic races from the Middle, North and South America. During the last four centuries, different ecotypes were developed through inter-crossing, selection and adaptation to new conditions on the territory of the former Yugoslavia.

Two groups of populations of a different origin, collected from various geographical regions of the country, were observed in this study with the aim to determine their interrelations.

The group of Montenegrin flints originates from the earliest introduced flint races from the Middle America that expanded widely over Montenegro and the Adriatic cost, as well as at higher altitudes. The group of populations classified as Derived flints was developed from the introduced types from the North America that spread over the Middle Europe, reached northern and western parts of the country and expanded further to the east and the south.

Based on both the morphobiological characterization and the multivariate statistic analysis it was determined that studied populations distinguished from one another, as well as that populations belonging to groups of different origin were genetically distant.

Regarding the importance of heterosis for maize breeding, these results could be used in developing synthetic populations, which could express heterosis when crossed. Populations of both the Montenegrin flints and the Derived flints could form a new heterotic pair in a pre-breeding programmed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC POTENTIAL OF SEED GERMINATION IN FORAGE CROPS CULTIVARS IN THE FIFTH YEAR OF THEIR LIFE

 

Z. Tomić, Lugić Z.. Sokolović D.

 

Agricultural Research Institute "Serbia", Center for Forage Crops, Krusevac

 

Genetic potential of seed vigor and germination declines with age. The aim of investigations was to establish genetic potential of seed vigor and germination in the most important and prominent forage crops. Elite seeds of new cultivars from the Center for Forage Crops, Krusevac were tested. Cultivars of perennial grasses were as follows: cocksfoot (Dactylis glomerata L.) cv. K-R. meadow fescue (Festuca pratensis Huds.) cv. K-21. Italian reygrass (Lolium multiflorum L.) cv. K-29 tetra and tall fescue (Festuca arundinacea Schreb.) cv K-20. Cultivars of perennial legumes were as follows: alfalfa (Medicago saliva L.) cv. K-22, red clover (Trifolium pratense L.) cv. K-32 tetra and white clover (Trifolium repens L.) cv. K-33. Seeds were planted on experimental plots of the Center in 1994 and harvested Hie following year. Seed germination was established by standard laboratory methods in the harvest year. The same samples were analyzed for germination successively per year and in the fourth year of their life, respectively. The results were processed by the analysis of variance and tested by LSD-test. In the harvest year the results for vigor and germination are beyond minimal requirements set by legal provisions for these species seed trade (85-95%). Seed vigor and germination declined statistically significantly with seed age. In perennial grasses, tall fescue cultivar retained highest seed germination (75%) after 5 years of life, while meadow fescue cultivar exhibited best seed germination (34%). The cultivar of cocksfoot retained its germination ability of 65% and Italian ryegrass of 67%. In legume cultivars very significant difference was found in seed germination per year of life. Seed germination of white clover was most reduced, even to 50%, while that of red clover (82%) and alfalfa (76%) remained at the germination level, when seeds can be also used in the fifth year. The results demonstrate that genetic potential of seeds of perennial grasses and legume cultivars is a critical factor that needs to be considered a limiting one in seed use value throughout further selection activities for creating new cultivars.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BREEDING SUNFLOWER FOR RESISTANCE TO DOWNY MILDEW (Plasmopara halstedi (Farl.) Berl. et Toni) AND BROOMRAPE (Orobanche cernua Loefl./Orobanche cumana Wallr.)

 

Branislav Dozet, Dragan Škorić, Radovan Marinković

Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad

 

The paper reports the results we have thus far achieved in introducing genes for resistance to downy mildew and broomrape into sunflower line HA-19. Our use of convergent crosses based on transgressive recombination has proven very suitable as a method for incorporating resistance genes into standard sunflower lines. The x2 test has shown the inheritance of resistance to be controlled by a single dominant gene. The results have also confirmed that the presence of broomrape in plant materials can be diagnosed very early in the season using a modification of the Pancenko method. In the present study, an assessment made 40 days after sowing showed that broomrape plants were for the most part well developed by that time.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETICS OF THE JUVENILE PHASE OF TREE GROWTH AND THE ASPECTS OF SHORT-ROTATION PLANTATION ESTABLISHMENT

 

Aleksandar Tucović, Vasilije Isajev i Milan Mataruga

 

Faculty of Forestry, Belgrade

 

            The complexity of tree organization their longevity, mechanism of reproduction, heredity, variability, senescence and their dependence on environmental factors result in the fact that we still do not have a generally accepted theory of tree ontogeny. The orientation to short-rotation plantations directs the genetic research to the juvenile stage of ontogeny. Simultaneous research of ontogeny at several levels, i.e. species, population, individual organisms and cells, is the base for a faster creation of the concept of tree ontogeny. Different levels of the study of tree ontogeny have some disadvantages but also they have some advantages, which will probably stimulate further, primarily experimental, research. A very significant property is the high potential of seeds, seedlings and juvenile plants compared to productive (superior) genotypes, whose multiplication results in a narrower evolution potential. The experiments in individual organisms and at the level of cells contribute to the enhancement of the concept of genetic control of ontogeny, which is based on molecular genetics and information theory.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STRATEGY AND PREVIOUS RESULTS OF PEDUNCULATE OAK (Quercus robur L.) BREEDING IN YUGOSLAVIA

 

Sasa Orlović1, Joška Erdeši2, Srbislav Radivojević2, Zoran Obućina2, Gojko Janjatović2

 

Agricultural Faculty Novi Sad, Poplar Research Institute. Antona Cehova 13, 21000 Novi Sad, Yugoslavia, E mail. sasao@polj.ns.ac.yu

2 Forest Enterprises "Srbijasume" Forest District Sremska Mitrovica

 

The forests of pedunculate oak (Quercus robur L.) are economically most valuable forests in Europe. In Yugoslavia they occur naturally in the valley of the river Sava, and less in the valleys of the rivers Danube and Morava. The breeding of this species in Yugoslavia started by selecting the phenotypically superior genotypes in the populations of Ravni Srem. The main criteria for the selection were the straightness of stem and low susceptibility to mildew (Microsphaera alphitoides). They were used in the establishment of the clonal seed orchard on 7 ha at the locality "Banov brod". According to the preliminary research of half-sib progeny from this seed orchard, they are characterized by heterotic growth. In the following period, we plan the selection of new genotypes from natural pedunculate oak populations, from which, together with the existing seed orchard "Banov brod", new seed orchards will be established.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

SELECTION OF DOMESTIC WALNUT (Juglans regia L.) FROM NATURAL POPULATION

 

M. Mitrović, M. Nikolić, D. Ogašanović, Z. Tešović

Fruit and Grape Research Centre, Cacak

 

Yugoslavia has an abundant population of different walnut biotypes from natural population. Current production is primarily based on these types. Many important walnut cultivars resulted from the types selection from natural population. Clonal selection, introduction of walnuts from all over the world and the grafting technology led to significant progress in walnut growing in Yugoslavia.

Since 1980, several dozen of most interesting walnut types from natural population from the whole territory of Serbia have been selected, evaluated and described at Fruit and Grape Research Centre, Cacak. They are all distinguished by differing traits: early, mid-early or late-flushing, excellent cropping, resistance to pests and diseases, lower susceptibility to winter frosts, fruit quality (size, kernel percentage, kernel colour, etc).

Some of the selected types have been presented in this paper: 8/81- Vraćevšnica, 32/82 - Donja Koritnica. 41-9/82 - Poružnica, 9/82 - Bogutovac, 1/90 - Kablar, 16/93 -Jezdina, 5/95 - Viča, and 7/98 - Prijevor.

Their major economic-biological properties are presented in the paper.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE GENETIC AND PHENOTYPIC VARIABILITY AND RELATIONSHIP OF SOME GROWTH AND SLAUGHTER CHARACTERISTICS OF THE PERFORMANCE TESTED GILTS

 

Nenad Brkić1, Živorad Gajić2, Milovan Pušić1

 

1 Institute for Scientific Research and Transfer of Technology in Agriculture PKB "INI -Agroekonomik", Beograd - Padinska Skela
2
Faculty of agriculture, Beograd – Zemun

 

Evaluation of the breeding values as a result of performance tested gilts was carried out at the farms "Vizelj" and "Ratari" and at the both farms the tested gilts achieved the body weight of 103.81 kg at the average daily growth rate of 0.491 kg within the period of 209.25 days. At the end of the test live animals were measured by ultrasound machine (PIGLOG 105) and it was found that the average backfat thickness was BT1 20.09 mm. BT2 15.25 mm and BTA 17.67 mm, the average muscle depth was MLD 45.25 mm and the average lean meat content was 52.92%. The gilts F1 generation Swedish Landrace x Large White (SLxLW) achieved better results concerning growth rate and lean meat content indications in comparison with the gilts purebreed Svedish Landrace (SL), while statistically significant differences were found out by the t-test (P<0.01) between the breeds all tested characteristics except for the backfat thickness SL1 and muscle depth (MLD), where the results were statistically nonsignificant (P>0.05).

High heritability is noticed with the characteristic indicating backfat BTL BT2 and BTA as well as the lean meat content (h2 0.561 to 0.776), while the depth of MLD shows low heritability (h2 0.077) and the daily growth rate shows medium heritability (h2 0.254). The established genetic correlations between the backfat thicknesses are complete (rg 0.972 up to 0.993) as well as between the backfat thickness and the lean meat content where the correlations are negative and complete (rg -0.988 up to -0.999), while between backfat thickness and MLD are low and negative (rg -0.192 up to -0.299).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INFLUENCE OF SYSTEMATIC ENVIRONMENTAL FACTORS ON GENETIC INDEXES AND RANK OF POTENCIAL SIRES

 

V. Bogdanović1, Brkić N.2

1 Department of Animal Breeding, Faculty of Agriculture, Belgrade-Zemun

2 PKB INI "Agroekonomik", Belgrade

 

In order to analyze influence of systematic environmental factors, data of 643 performance tested beef bulls were used. Three genotypes of Italian beef breed (Marchigiana. n=181; Chianina. n=240; Romagnola. n=222) have been covered by this research. Observed traits were average daily gain during the test and estimation of meatiness. Different sources of variation (breed, type of rearing, farm, group, twinning and parity) were included in two statistical models. Both models showed a very high statistical significance (P<0.001). Coefficients of determination of models were influenced by included factors and their range varied. In according to applied models a different value of genetics indexes were obtained. Main result of those differences was a different rank of bulls. The highest difference was obtained in rank for average daily gain during the test. It was concluded that the adequate determination of non-genetic source of variation in model might be of final importance for estimation of genetics index and rank of potential sires.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

UVOLOGICAL AND TECHNOLOGICAL INVESTIGATION OF CLONE POPULATION IN CULTIVAR KABERNE FRAN

 

Avramov L., Milutinović M., Jović, S., Žunić D., Maletić Radojka, Vujović D.

 

University of Belgrade- Agricultural Faculty – Zemun

 

In the Radmilovac region, at the Grocka vinedistrict, investigation of the F3 clonal generation of the cultivar Caberne Fran has been investigated. Examination was done during die period 1994 - 1998. The aim of this experiment was to create commercial clones of this cultivar. Uvological and technological investigations were done in two phases, and included following indexes: (a) phase I - During the period 1994 - 1998 following characteristics has been investigated: cluster weigh, number of clusters, grape yield, sugar content, total acid content: (b) phase II - During the period 1997 - 1998 mechanical analysis of cluster and berry with the following characteristics has been done: cluster composition, berry composition and cluster structure. During the II phase, wine characteristics have been investigated, and included following indexes: alcohol, extract, total acid content, sugar, ogranoleptical evaluation. Results of investigation showed that between examined clones existed very significant differences in majority of characteristics. Results were processed because of establishment of significance of differences. Following standard statistical methods were used: mean values, coefficient of variance, analysis of variance, LSD test, discriminate analysis, etc.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CYTOGENETIC STUDY OF THE SPECIES Helianthus rigidus AND ITS F1 HYBRIDS WITH THE CULTIVATED SUNFLOWER, Helianthus annuus

 

Jovanka Atlagić

 

Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad

 

Helianthus rigidus (Cass.) Desf. is a hexaploid sunflower which is a potential source of resistance to pathogens and high protein content in seed.

We have crossed many populations of the species with lines of the cultivated sunflower and we obtained four F1 hybrid combinations (1-9 plants in each) and five BC1F1) combinations (1-11 plants in each).

Male sterility was registered in F1 and BC1F1 interspecific hybrids. Pollen viability was reduced in the progenies as compared with the parents (27 57-71.83% in FI and 2.41-72.63% in BC1F1).

The meiosis preceded normally in the H. rigidus populations under study. Numerous irregularities occurred at meiosis in the F1 hybrids. At diakinesis, bivalents prevailed (88.29% of the meiocytes) but quadri-, uni- and hexavalents were also noted. Dislocated chromosomes and chromosome bridges were present in the other meiotic stages. All F1 plants had 68 chromosomes each. In the case of BC1F1 hybrids, high percentages of uni-, quadri- and hexavalents occurred in meiocytes at diakinesis. BC1F1 plants differed in chromosome numbers.

The registered meiotic irregularities in F1 and BC1F1 interspecific hybrids between H. rigidus and H.annuus are indicative of genetic divergence, i.e., of non-homology in the sunflower genome.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC VARIABILITY AND MODE OF INHERITANCE OF YIELD COMPONENTS IN MAIZE INBREED LINES AND ITS HYBRIDS

 

Jan Boćanski1 i Zoran Petrović2

 

1 Faculty of Agriculture and Institute of Field and Vegetable Crops, Novi Sad

2 Institute of Field and Vegetable Crops, Novi Sad

 

Genetic variability was studied in twelve, maize inbreed lines for number of grain rows per ear, ear diameter and grain yield per plant. The mode of intheritance in F1 generation also was investigated, where standard statistical methods were used.

Obtained results in hybrids confirmed intermediary, parcialdominance, dominance and superdominance as a mode of inheritance in number of grain rows per ear. Superdominance was recorded in ear diameter and in grain yield per plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENE-FOR -GENE MODELING FOR WHEAT HYBRIDS RESISTANT TO PUCCINIA RECONDITA TRITICI

 

Jelena Bošković1, Bošković M.2, Pešić V.1

 

1 Faculty of Agriculture, Zemun-Beograd, Nemanjina 6, 11030 Zemun

2 Faculty of Agriculture, Novi Sad Trg D. Obradovica 8, 21000 Novi Sad

 

It was essential to found the methods for genetic differentiation of sources of resistance of wheat for our new differential of sources of resistance of wheat for our new approach in international survey of Puccinia recondita tritici Boolean modeling in gene-for-gene relationship was applied in genetic differentation. The methods of analysis included the infection type data, aegricorpus phenotypes which indicate aegricorpus, parasite and host genotypes in parasite: host: environment specificity,.

The hybrid wheat lines with two pairs of resistance genes from the program of accumulation of resistance genes to P. recondita tritici and conventional ratios 9:7 have been tested with three pathogen cultures.

63The lines NS-77/lxLr9, NS-26/lxLr9, NS-26/2xLrl9, NS-46/2xLr9, NS-94/2xLr9, NS-94/4xLrl9, with the pathogen culture Bg.s. 12/89, the lines NS-66/2xLr9, NS-77/l x Lr9, NS-37/3 x Lr24,NS-94/5 x Lr24 with pathogen culture Is.w./89, and the lines NS-32/1 x Lr9 i NS-46/3 x Lr24 with pathogen culture Chl.w. 14/89, with pathogen culture Clil.w. 14/89.

It is applied an expansion of Model II for listed hybrid combinations with two sets of corresponding gene pairs occurring in definitive (constant) environment, as well as in different environments. From the results obtained, it was clear that inclusion of different environment in the Model would alter the phenotypes ratios, but not genotype ratios. That means, the number of definitive genotype remain the same, whether or not environment is considered.

From the other side, the application of the gene-for-gene Models have shown much advantages toward conventional methods, since the slightest increase in either V or N results in a very large number or possibilities in genetic differentiation of sources of resistance.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY AND HERITABILITY OF PLANT HEIGHT COMPONENTS IN SEVERAL DOMESTIC VARIETIES OF BEAN (Phaseolus vulgaris L.)

 

Mirjana Vasić, Jelica Gvozdanović Varga. Janko Červenski

 

Institute of Field and Vegetable Crops, Novi Sad

 

Investigated in our three-year study were plant height, height of first pod formation, and productive plant height in ten domestic bean varieties. The study was carried out on bean varieties with colored grain (Zlatko, Sremac, and Slavonski Zeleni) and white grain (Biser, Medijana, Caleb, Panonski Gradistanac, Panonski Tetovac, Dvadesetica, and Belko). The greatest plant height was found in Biser, followed by Medijana, and Panonski Tetovac. The variety Caleb had the smallest height of first pod formation (14.4 cm). All the other genotypes formed the first pod at heights of more than 15 cm. The smallest productive heights were recorded in the colored varieties. The variability of the studied traits was not high. The analysis of variance suggested that the variety and year influenced all the components of plant height. Similar values of the coefficients of genotypic and phenotypic variation indicated that the traits were equally affected by genotypic and phenotypic factors. The presence of genetic heterogeneity for productive plant height in the varieties was confirmed by higher genetic coefficient of variation. Broad sense heritability values for all three traits were high.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

RAPD ANALYSIS OF PRODUCTS OF FUSION BETWEEN CULTIVATED SUNFLOWER AND Helianthus maximiliani (Schrader)

 

Dragana Vasić, Dragan Škorić, Gilbert Alibert*

 

Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad

*INP-ENSAT/UA INRA, Avenue de 1'Agrobiopole, BP 107, 31326 Castanet Tolosan, Cedex, France

 

In order to confirm hybrid nature of products of fusion between cultivated sunflower and H. maximiliani PCR analysis of regenerated calli and somatic embryos was done using RAPD markers characteristic for H. maximiliani. Presence of DNA fragments of this wild species was detected in 80% of analyzed embryos and 50% of analyzed calli. Since percentage of heterofusions was found to be 23% it could be concluded that culture conditions favour development of heterocaryons.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

MUTUAL DEPENDENCE AMONG COMPONENTS OF GREEN FORAGE YIELD IN VARIETIES AND POPULATIONS OF RED CLOVER (Trifolium pratense L.)

 

Sanja Vasiljević1, Gordana Šurlan- Momirović2, S. Katić1, V. Mihailović1, D. Lukić1, T. Živanović2

 

1 Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad

2 Faculty of Agriculture, Zemun-Beograd

 

Analyses of variance and covariance have been used to assess coefficients of genetic and phenotypic correlations for components of green forage yield in varieties and populations of red clover. The intention was to establish relationships among the characteristics studied. The study included 17 varieties and 16 populations of red clover in the second year of growing. Samples (30 plants per genotype) for the analyses of components of green forage yield were taken at the stage of flowering of first umbels in the first cutting. Randomly selected 30 plants, i.e., 150 flowers per genotype, taken at the stage of full maturity of seed in the second crop, were used to assess the following characteristics: number of productive stems per plant, number of flowers per plant, number of flowers per umbel, number of seeds per umbel and seed yield per plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECT OF SYSTEMATIC FACTORS OF THE ENVIRONMENT ON THE VARIABILITY OF MILK PRODUCTION

 

Mirjana Vulić


Faculty of Agriculture, Novi Sad

 

Data about milk of Black and White cows during the first lactation served for appraisal of systematic effects of the environment. For that appraisal the least square (LS) method was used as defined by Harvey. There were 675 first lactations of cows originating from 18 sires, included in the investigation. Milk yield in first 100 days of lactation was on average 2319 kg, and in the whole lactation 6186 kg, while in the standard lactation it was 6058 kg. Butterfat content was 3.11, 3.22 and 3.21 per cent, respectively. The year and season of calving, as the farm also, significantly affected (P<0.01) on the milk yield, butterfat yield and content for the first 100 days, whole and standard lactations, respectively. The highest milk butterfat yield above general mean for first 100 days in the whole and standard lactation was achieved in cows calved in 1994. A positive effect on the butterfat content in the first 100 days, the whole and standard lactation achieved the cows calved in 1995. The cows which calved during the winter months (season I) had a higher milk and butterfat yield in relation to mean in the first 100 days in the whole and standard lactation. For the butterfat content the most favorable was the fourth season. The highest positive deviation from the mean of milk and butterfat yield, as well as on butterfat content in the first 100 days, the whole and standard lactation was in the farm 3.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

DIVERGENCE OF GENOTIPES FOR DRY MATTER YIELD IN GARLIC

 

Jelica Gvozdanović-Varga, Mirjana Vasić

 

Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Yugoslavia

 

In this study we investigated the divergence of garlic genotypes regarding dry matter yields and production characteristics. Garlic genotypes had been collected around the Vojvodina province to study distribution and evaluate traits. According to the yield level, they could clearly be divided into autumn garlic and spring garlic. On the basis of cluster analysis (k-mean), furthermore, they were divided into five groups. The first group consisted of spring garlic ecotypes with the lowest dry matter yields and a dry matter content of 40.24%, the second of genotypes (both spring and autumn) with the highest dry matter yield (4.85 t/ha) and a very high dry matter content, and the third of the highest-yielding genotypes. Ecotypes with the highest yields did not also have the maximum yields of dry matter (3.52 t/ha). The results of cluster analysis enabled us to differentiate between genotypes for different breeding purposes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF SERBIAN SPRUCE (Picea omorika /Panč./Purkyne) POLLEN GERMINATION PERCENTAGE DURING 12-MONTH STORAGE

 

Branislava Grbović1, Vasilije Isajev2

 

1 S.E. "Srbijasume" - Institute of Forestry, Belgrade, Kneza Viseslava 3, 11030 Belgrade

2 Faculty of Forestry, University in Belgrade, Kneza Viseslava 1, 11030 Belgrade

 

This paper presents the results of the analyses of individual variability of Serbian spruce pollen, as well as variability of its germination percentage during 12-month storage in varying conditions. During two successive years pollen was collected from 25 test trees in Serbian spruce seed orchard at "Bela Zemlja" near Uzice (Serbia). During 12 months, by Kobel's in vitro method on 6 nutritive media, we analyzed the germination percentage of pollen kept at four different temperatures. The results of individual selection, as well as the analyses of the course of variability of pollen germination percentage and its potential conservation are significant for the study of Serbian spruce genetic cycle, as well as for hybridization purposes in the breeding program of this significant species.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POPLAR SELECTION FOR SUPERIOR GROWTH

 

Guzina V., Orlović S., Kovačević B.

 

Poplar Research Institute, Faculty of Agriculture, Novi Sad

 

In the test with thirteen families of hybrid progenies of eastern cottonwood (Populus deltoides Bartr.) and its hybrids with selected clones of Euramerican poplar Populus deltoides x Populus x euramericana, measured values of tree dimensions were analyzed and wood volume per ha after the sixth, ninth and eleventh year in the test were estimated. Significant differences between the progenies were determined in tree dimensions and estimated wood volume per ha, as well as a significant variability of these elements within the progeny. The above values were compared with the corresponding values of parent trees and with the trees of registered poplar cultivars grown as standards in the same test. Potential selection of clones with higher potentials of fast growth compared to registered cultivars of black poplars was discussed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

IDENTIFICATION OF DONOR LINES FOR IMPROVING FRUIT YIELD OF K35 x K12 EGGPLANT HYBRID

 

Jelena Damjanović1, Maja Vračarević2, Bogoljub Zečević1 i Slaven Prodanović3

 

1 Center for Vegetable Crops, Smederevska Palanka

2 INEP, Zemun

3 Faculty of Agriculture, Zemun

 

The aim of this study was to identify donor line possessing the largest frequency of favorable alleles for fruit yield which should be used to improve an elite eggplant hybrid K35xK12. The fruit yield in elite hybrid, its parents and their hybrids with tree potential donor lines (K36/1, K11 and K22/2) was studied. The trials were set in Center for Vegetable Crops, Smederevska Palanka, in three replications. After measuring the fruit yield, the modified method for evaluation of relative loci values according to Dudley (1987) was applied. It was found that all lines had positive values of (μG' parameter. Line K11 had the largest value (2.38) and the lowest frequency (1.33) of unfavorable alleles on D class loci. It was also established that this line is more related to K35 parent (+7.81), so on the basis of μD' values, improvement should be done by backcrossing the elite hybrid. Hybrid progeny parent P1 (K35) x donor (K11) should be backcrossed with P1 to conduct selection and chose such genotype which will create more yielding fruits by hybridization with K12 line.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PROTEIN MARKER POLYMORPHISM IN SET OF MAIZE INBRED LINES

 

Gordana Demić, Snežana Mladenović Drinić i Kosana Konstantinov

Maize Research Institute, 11080 Zemun Polje-Belgrade

 

Embryo salt soluble proteins were isolated |from 15 maize genotypes, including both public lines and inbreds created at Maize Research Institute "Zemun Polje". Association among analysed inbred lines has been determined from cluster analysis based on protein marker date. The UPGMA clustering method was used for hierarchical clustering and the necessary computation were perform using NTSYS-pc program. The protein based dendogram for 15 analyzed inbred lines, consisted of three major groups. First group consists of inbred lines derived from or related to BSSS germplasm; second group belong to Lancaster germplasm inbreds and third group are two public French inbred lines F2 and F7. The BSSS group consists of three subdivisions: B73 and B84; B73 Sbms and B73M; and A632 with L362. Inbreeds L326 and L227 were loosely aggregated with B73 and A632 related lines. Within the Lancaster group inbreeds association in two subdivisions of related lines have been obtained: lines L385 and Mol7 clustering together in one subgroup, while L395 and its progenitor L412 were in another. Grouping of inbreeds revealed by the present analysis generally agreed with the pedigrees of these lines.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ALFALFA: FROM POPULATIONS TO VARIETIES

 

Dragan J. Bukić, Dane Lukić

 

Faculty of Agriculture, Institute of Field and Vegetable Crops, Novi Sad

 

The paper reviews the development and most important quantitative traits of the nine alfalfa varieties developed over last 50 years at the Institute of Field and Vegetable Crops of the Faculty of Agriculture in Novi Sad. The new varieties were developed mostly from indigenous materials - the Pannonian alfalfa type (Medicago sativa L.) and a population of yellow alfalfa (Medicago falcata L.). In long-term performance tests conducted in several locations and under different soil and climatic conditions, the new varieties exhibited a high potential for the yields of green forage and dry matter (73.3 t/ha and 18.3 t/ha, respectively) and high quality of dry matter (crude protein content of 18.74%).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CHANGES ON PROTEINS IN DIFFERENT SOYBEAN VARIETIES UNDER EFFECTS OF INCREASED TEMPERATURE

 

Slađana Žilić, Irina Božović, V. Bekrić i Snežana Mladenović Drinić

 

Maize Research Institute, Zemun Polje, Zemun-Belgrade

 

Soybean proteins are mainly globulins. The seed proteins were separated into three major fractions with the average sedimentation coefficients of 2,2S, 7,5S and 11,8S. They were also encoded by the nuclear genome in the cytoplasm. Joint segregation of 1 IS subunit presence and absence fitted the theoretical ratio for independent inheritance among the three loci controlling subunits of the group I and subgroups IIa and IIb. The DNA fragments and complementary mRNA which given synthesis of the subunits of the 7S soybeans seed protein were extensively characterized. Soybean is one of the best sources of high quality plant protein. However, it also contains various antinutritional factors. In order to destroy proteolytic-inhibiting substances and urease it is necessary to heat the row seeds of soybean.

Seeds of genetically different soybean varieties (Brock and Kunitz) were used in this study. Changes on proteins after heating soybean seeds in the microwave oven observed in the experiments.

Based on the obtained results it can be concluded that the microwave heating caused almost identical changes of chemical composition in seeds of both observed varieties. Heating soybean seeds for 1, 2, 3, 4 and 5 minutes did not affect content of total proteins, but their denaturation after 2 minutes of heating was noticed. The content of water soluble proteins was reduced up to the 3rd minutes in both soybean varieties, but the maximum drop of solubility of 16.84% and 12.69% was noticed, in the varieties Brock and Kunitz after 2 minutes of heating.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

FLOWER TYPE IN F1 GENERATION RECEIVED BY HYBRIDISATION OF GRAPEVINE VARIETIES - DRENAK CRVENI X AFUZ-ALI

 

Žunić P., Bešlić Z.

 

Faculty of Agriculture, Beograd

 

The flower type is important biological characteristic of grapevine that exerts an influence on height and stability of yield. The low yield is result of loose grapes in cause of the female flower type. The varieties with female flower type do not have possibility of self-fertilization and their yield depends on presence of pollinator's pollen. One of the variety with female flower type is Drenak Crveni and it has high yield and quality' of grapes in case of optimal fertilization. The hybridization of Drenak Crveni and Afuz-ali was performing to create a new variety with hermaphroditic flower and better aerobiological characteristics. The result of researches shows that there are 42,5% seedlings with hermaphroditic flower and 51,5% with female flower. We received 11 plants with intermediate flower (female with upright pistils) in group with female flower. This results obtained prove the thesis of monofactorial determinism of the flower types in grape varieties. The varieties with female flowers are homozygous (FF) for that attribute. Pollinated by hermaphrodite heterozygous varieties they give in progeny approximately the same ratio of hermaphrodite and female plants.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECTS OF GENETIC PARAMETERS ON INHERITING THE NUMBER OF PODS PER PLANT IN BEANS (Phaseolus vulgaris L)

 

Zdravković M., Čorokalo D., Đorđević R. i J. Zdravković

 

Agricurtural research Institute "Srbija", Centre for Vegetable Crops, Karađorđeva 71, 11420 Smederevska Palanka cfvcsp@eunet.YU

 

We investigated four divergent bean genotypes (Biser, Caleb, Rozalija and Palanacki zlatno zuti) as well as their F, descendants obtained by diallel crossing - without reciprocal. The research was aimed at investigation of their mode of inheritance, gene effects, combining abilities and components of genetic variance.

As for the feature number of pods per plant, we recorded a highly significant variance of mean values of the parents and the hybrids. The calculated values of the additive component (D), were higher than the dominant components (H1 and H2) which suggested that the additive genes were prevailing for the feature inheriting the number of pods per plant. The mean degree of dominance (H1/D) was lower than 1, suggesting partialdominance. The highest value of specific combining abilities was recorded in the combination Biser x Rozalija. Heritability in broad sense was 95%, indicating high genetic factor effects in inheriting number of pods per plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INHERITANCE MODE OF FRUIT SHAPE IN TOMATO

 

Jasmina Zdravković, Marković Z., Zdravković M., Mirjana Mijatović

 

Agricurtural research Institute "Srbija", Centre for Vegetable Crops, Karađorđeva 71,

11420 Smederevska Palanka, cfvcsp@eunet.yu

 

By diallel crossings of 6 parental genotypes (D-150, S-49, S-35and H-52, Kg-z and SP-109) and their descendants obtained from these crossings (F1, F2, BC1 and BC2), we investigated the ratio between the horizontal and vertical diameter of tomato fruit which was represented by the index, i.e. tomato fruit shape. The inheritance mode of fruit shape in tomato was determined by the mean generation analysis (Mather and Jinks, 1982). The additive gene effects were the most significant in inheriting the fruit shape. Most frequently expressed effects were additive x dominance interaction, followed by additive x additive gene effects. The duplicate type of epistasis was relatively stable.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INHERITANCE OF YIELD COMPONENTS IN DIALLEL CROSSING OF DIVERGENT GENOTYPES OF PEPPER (Capsicum annuum L.)

 

Bogoljub Zečević1, Dušan Stevanović1, Slaven Prodanović2 i Radiša Đorđević1

 

1 Centre for Vegetable Crops, Smederevska Palanka

2 Faculty of Agriculture, Beograd

 

On the basis diallel crossing (without reciprocal) of six divergent genotypes of pepper (Palanacka babura, Macvanka, Palanacka kapija, Romana, Kobra i Feferona zuta ljuta), the mode of inheritance and the components of genetic variance were analysed for fruits weight and number of fruits per plant. The investigation was based on data of F1 generation. In the most of hybrids, the partial dominance was estimated as the mode of inheritance for both characters. The analysis of components of genetic variance has showed that the main part of genetic variance belongs to the additive gene effect for yield components .The high values of heritability indicated also more important role additive genes. On the basis calculated results it can be conclude that in the breeding of pepper for yield, it should be select of genotypes with high average values for fruit weight and number of fruits per plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY AND COMPONENTS OF VARIANCE FOR PLANT HEIGHT IN DIFFERENT WHEAT CULTFVARS

 

Veselinka Zečević, D. Knežević, Danica Mićanović

 

ARI SERBIA. Center for Small Grains, Kragujevac, S. Kovacevica 31, 34000 Kragujevac

 

In this work was analyzed height of plant in 50 divergent wheat cultivars. The cultivars were grown in experiment designed by randomized block system in three repetitions during two years on experimental field of the Center for Small Grains Kragujevac. The sowing of seed were done in rows length 1 m, 20 cm distance between rows and 10 cm between seeds in a row. Plants (60 plants in three repetitions) were analyzed in stage of full physiological maturity. The variability, heritability and components of variance for plant height in different wheat cultivars were done. Significant differences for average values for plant height in analyzed wheat cultivars were established. The highest height of plant was established in Blue Boy cultivar (122.2 cm) and the lowest in the Norm 10 cultivar (50.4 cm). Variability of plant height have been rather low which coefficient of variation was in average (V=7.4 %). Heritability in broad sense for plant height was h2=95.9 %, indicating high inheritance of this traits. Components of phenotypic variance show that in total variability of this trait the highest impact belongs to genotype (84.3 %), less impact belongs to year (7.5 %) and interaction cultivar/year (6.7 %).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

APPLICATION OF INTRODUCTION AS A METHOD OF TREE BREEDING -THE EXAMPLE OF BLACK WALNUT (Juglans nigra L.)

 

Vasilije Isajev1, Predrag Jović2

 

1 Faculty of Forestry, Belgrade

2 Institute of Forestry, Belgrade

 

American black walnut (Juglans nigra L.) is a potentially significant species of hard broadleaves on alluvial soils adjacent to the banks of major rivers in Serbia. In addition to excellent technical characteristics, health state of the plantations and the price of walnut timber at the market, this species is also characterized by an excellent volume increment, and thus also the volume it yields in a relatively short time period, which is not the characteristics of forestry. Unjustifiably small acreage of black walnut in our country should be extended to all optimal sites for its successful production. Care should be taken about the "population founder", i.e. a drastic reduction of the gene complex and the conversion of the existing populations (cultures) from open into closed populations. As the future of genes in populations depends on the combination of population size and selection pressure, selection pressure in actual populations can be increased by the establishment of plantations on a non-uniform site (to minimize the genetic effects), by a more rigorous selection (only of the best individuals), by raising the progeny of selected individuals and by the selection based on progeny tests. It should be taken into account that the analysis of half-sib progeny tests in selection programs for this species in USA shows the inheritance of height and diameter amounting to 0.54 and 0.50 (i.e. significantly higher inheritance of these parameters than other commercial species). This should be the reason of a more exacting work on black walnut improvement in our country. This paper presents the way and the method of black walnut improvement program in Serbia.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF MACROSCOPIC CHARACTERISTICS OF SERBIAN SPRUCE (Pices omorika /Panč./ Purkyne) NEEDLES IN ECOLOGICALLY DIFFERENT MICROPOPULATIONS

 

Vasilije Isajev1, Vera Lavadinović2

1 Faculty of Forestry, Belgrade

2 Institute of Forestry, Belgrade

 

Three micropopulations of Serbian spruce were set aside on Mt. Tara and one in the town square at Uzice, in order to assess the scope of the effect of parent rock on the number and sizes of second-year needles. The selected populations on Tara are situated on marshy terrain, calcareous bedrock, serpentine and at Uzice on an anthropogenically modified site in urban conditions. Twenty test trees were selected in each population and the needles were collected for the analysis. The analysis included the variability of needle number per 1 cm of second-year branchlet, and needle length and width was analyzed on the statistical sample of 20 needles from each test tree. Based on statistically justified differences between mean values of the analyzed characters, it can be concluded that the effect of parent rock on the variability of study characters is not high; it ranges between 8.1 and 9.1%. The scope of variation of study characters is affected by a great complex of factors. As the needles are of the same age and collected from the trees at the same elevation and exposure, it can be concluded that the scope of variation of study characters is under a strong genetic control.

The results of the morphometric analysis show that study characters had the highest values in urban, anthropogenically changed conditions, and not at their natural sites. This is probably the consequence of the interaction of tree genotype from this population and the effect of intensive tending by watering, fertilization, etc.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CHROMOSOME ABERRATIONS AS A CAUSE OF DECREASED FERTILITY IN FEMALE PIGS AND CONGENITAL MALPHORMATIONS IN OFFSPRING

 

I. Jevtić, Z. Stanimirović, B. Soldatović

 

Cytogenetic analysis (monitoring of numerical and structural aberration frequencies) was examined on female pigs of different ages (from three to ten partition). In addition their normal and pathologic offspring was also analysed.

In order to perform cytogenetic analysis the whole blood of females was used to start lymphocyte cultures (Evans and O'Riordan, 1977, slightly modified by Zimonjic, 1990).god.). As for the piglets cytogenetic analysis (Atresia ani, Cheliognathopalathoschisis, Extremitas ad larus, Flexia phalangis distalis extremitas) was performed on bone marrow cells (Hsu and Paton 1969. modified by Stanimirovic 1995)

The obtained results point to the fact of increased structural aberrations (deletions, translocations - both reciprotial and Robertsonian) in function of the female pig age and number of parturitions.

 

 

Ordinal number III        IV        V         VI        VII       VIII      IX        X

of partition

 

% total

karyotype          2,29      5.85      6,96      12,66    32,22    33,93    45,85    62,30

aberrations

 

Cytogenetic analysis described above point to the increase of nonvital piglets in the offspring dependent of ordinal number of partuition. Also, we observed that piglet malphormations are correlated with structural aberrations found both in the offspring, as well as in piglets itself. Thus, after the analysis of piglet cariotypes with such malformations we established direct relationship between the anomaly and certain chromosomal changes:

 

ANOMALY                                       MARKER CHROMOSOME
Cheliognathopalatlioschisis                    deletion 13q-
Atresia ani                                          reciprocal translocation t; (lq-;15q+)
Ekstremitas ad latus                             Robertsonova translokacija Rb;(14/15)(14+)
Flexia phalangis distalis extremitas        reciprocal translocation t; (4q+: 14q-)

 

On the basis of the obtained results we concluded that the age of female pigs inluences the occurrence of chromosome aberrations both in females and in their offspring, as a result nonvitality of piglets was observed as well as increased number of piglets with congenital malphormations.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC ANALYSIS OF PARENTS IN WHEAT BREEDING TO OBLIGATE PARASITES RESISTANCE

 

Zoran Jerković, Radivoje Jevtić

 

Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Yugoslavia

 

Base for the successful breeding for the resistance to obligate parasites of wheat is difference between parents according to genes controlling the characters. Number of genes and their interactions related to any type of the resistance are most important for the durability of the mentioned characters.

In order to create new lines with different base of Puccinia recondita and Erysiphe graminis resistance, two completely resistant lines in the seedling stage (controlled conditions) and in the field conditions were chosen. The second parent was Valjevka, variety with determinated level of uncomplete resistance to both parasites. Completely resistant parents to leaf rust couser are different from Valjevka in three independently inherited, complementary, dominant genes, KM 175/89 (resistant combinations A_B_, A_C_, B_CJ and KM 54/89 (A_B_C_). To powdery mildew couser, first completely resistant parent contains two independently inherited genes and the second one three with complement effect (A_B_, A_C_). In the progenies was transgressive segregation in direction of increasing of uncomplete resistance to parasites. Resistance expressed by differences in reaction types was not characteristic of older generations (F4 and F5). Enhancement of the uncomplete resistance can be explained by new interactions. Ten different lines according to resistance was created.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CALLUS INDUCTION FROM MATURE EMBRYOS IN SPECIES OF THE GENUS Triticum L.

 

Jestrović Zorica1, Kondić Ankica2, Šešek S.2, Denčić, S.2, Pavlović, M.1

 

1 ARI SERBIA, Center for Small Grains, Kragujevac, S. Kovacevica 31, 34000 Kragujevac

2 Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Yugoslavia

 

Callus formation and plant regeneration of mature embryos of Triticum monococum, Triticum dicoccoides, Triticum durum, Triticum spelta and Triticum aestivum were estimated. The embryos were inoculated on a modified MS (Morashige and Skoog, 1962) induction medium. The frequency of callus induction was in generally liigh (99.4% in average). The greatest number of initiated calli (100%) was obtained in Triticum durum and Triticum aestivum, while the lowest value (98.2%) founded in Triticum dicoccoides. The highest number of regenerated plants (51.87%) has been achieved by the species Triticum monococcum. Without regenerated plants was Triticum spelta. The average values for fresh weight of induced calli were ranged from 0.04 g in Triticum dicoccoides to 0.304 g in Triticum durum.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECT OF GENES AND COMBINING ABILITY FOR KERNEL YIELD IN SOME SUNFLOWER INBRED LINES

 

Joksimović J., Atlagić J., Škorić. D.


Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad

 

Effect of genes and combining ability for kernel yield have been studied in eight genetically divergent sunflower inbred lines and 15 F1 hybrids using the method line x tester (Singh and Choudhary, 1976).

Heterosis for kernel yield occurred in all F1 hybrids. The inbreds HA-BCPL and RHA-178 were the best general combiners for this trait. The values of specific combining ability (SCA) for the trait were found to depend on the ecological factors prevailing in the different test years. A significant positive SCA value for kernel yield was found only in the combination HA-74 x RHA-N-K. a cross whose parents had negative GCA for the trait under study.

The GCA/SCA ratio indicated that the non-additive gene action prevailed over the additive one, as confirmed by the analysis of the components of genetic variance.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC CONTROL OF MAIZE GRAIN DRY-DOWN DURING MATURATION

 

B. Kerečki, M. Ivanović i Violeta Anđelković

Maize Research Institute, Zemun Polje, Belgrade-Zemun

 

A great attention is paid to grain dry down during maize maturation in the process of inbred lines and hybrids development.

The grain maturation period can be divided into three stages. A soft dough stage is the first one with the grain moisture content of 65-70%. The normal moisture content in this stage is maintained on the basis of active metabolism. The second stage is known as grain filling and grain moisture decreases to 30-40%, while the third stage is characterised by the rapid loss of water and cessation of metabolic matter accumulation.

A dry down rate from maize grain depends on a few following parameters: osmotic pressure in grain, pericarp thickness and permeability, content and structure of starch granules, morphological properties of ear and grain, etc. The dry down rate from maize grain was observed in 12 inbreds and their combinations. Significant differences in grain moisture content were observed between inbreds and their combinations.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENE EFFECTS FOR PHYSIOLOGICAL PARAMETERS IN WHEAT

 

Kraljević-Balalić Marija

 

Faculty of Agriculture, Institute for Field and Vegetable Crops, Novi Sad

 

In order to analyze the mode of inheritance, gene effects and combining ability different number of genetically divergent parents were chosen. The parents (4-6) were diallel crossed, excluding reciprocals. On the basis of the results of F1 and F2 generations the gene effects for tiller number, leaf area, leaf area index (LAI) and leaf area duration (LAD), leaf position, pigments content, harvest index (HI) and a-amylase activity wer evaluated.

The cultivars and hybrids differed significantly in physiological parameters fc examined characters. ANOVA for combing ability, using Griffing's approach (method 1 model I) showed the total leaf area in wheat was under the control of additive genes in both generations. Highly significant general combing ability (GCA) and specific combining ability (SCA) variances were obtained for tillering, LAI and LAD, flag leaf angle carotenoid content, HI and a-amylase activity, indicating the presence od both additive an non-additive component of genetic variance. Chlorophyll content was caused only by non additive genes.

The importance of the results on gene effects, mode of inheritance and combining: ability of physiological parameters for wheat breeding is discussed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF SOME STRUCTURAL AND PHYSICAL PROPERTIES OF BLACK LOCUST (Robinia pseudoacacia L.) WOOD

 

Bojana Klašnja, Špiro Kopitović, Saša Orlović, Zoran Galić

 

Agricultural Faculty Novi Sad, Poplar Research Institute, Antona ehova 13, 21000 Novi
Sad, Jugoslavija, E-mail: sasao@polj.ns.ac.yu

 

This paper presents the results of the research of variability of the main structural (annual ring width and fibre length) and physical properties (density - basic and oven dry) wood of 10 black locust (Robinia pseudoacacia L.) clones. The samples of increment cores were taken from a field polyclonal test established in 1983 at the Poplar Research Institute Test Field on fluvisol soil type. The study samples were taken when the plantation was six, eight and thirteen years old. The clones were statistically highly different regarding the study properties. The values of fibre length and annual ring width were also statistically highly different per study years. Interclonal variability was very pronounced, which is shown by the results of cluster analysis grouping die clones into five groups. The coefficients of broad sense heritability for analyzed properties per ages ranged between 0.31 and 0.98. The results point to the need of further research in the adult phase in order to create a method of early selection for desired physical properties of wood.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

LEVEL OF GENETIC DETERMINATION OF SMALL GRAINS AND FUTURE PROSPECT

 

Desimir Knežević

 

ARI SERBIA, Center for Small Grains Kragujevac, Save Kovacevica 31, Kragujevac 34000

 

The extensive genetic investigation of wheat, barley, oat, rye and triticale gave great contribution for resolving of: polyploids of these plant species, genes control of morpho- physiological traits, productive traits and traits of technological quality, regulation of numerous biochemical processes etc. Up today, the knowledge obtained on the base of genetic investigation have played important role for taxonomy discrimination and evolutionary studies of small grains and their identification. The methods for identification of gene a gene alleles encoding expression of biological traits of already named plant species were developed. The determined genes for certain traits were used for cultivar identification within each plant species. The genetic and Physical mapping of cereal chromosomes was done. In this paper we will give review of genetic investigation of Yugoslav cultivars of small grains. The extensive investigation on land of cytogenetics, population genetics, quantitative genetics, mutagenesis, biochemical genetics etc., was conducted. Numerous cultivars were identified on the base of genes: for stem height (Rht, Saitama) which connected with lodging resistance, than genes encoding technological quality of plant species (Gli-1, Gli-2, Gli-3, Glu-1, Hor1, Hor2), disease resistance genes (Sr, Lr, BYDW) and pests as well as according to presence of IRL.IBS translocation. In some Yugoslav cultivars, nif genes and genes encoding tolerance to abiotic stress were identified. However, cultivars characterization on the DNA level is on the beginning. Obtained results indicting that our investigation was followed modern investigation and gave contribution to the world genetic investigation.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC DETERMINATION OF TECHNOLOGICAL QUALITY OF WHEAT

 

Desimir Knežević, Veselinka Zečević, Milanko Pavlović, Danica Mićanović, Miroslav Kuburović, 1Slaven Prodanović

 

ARI SERBIA, Center for Small Grains Kragujevac, Save Kovacevica 31, Kragujevac 34000

1 Agricultural faculty Zemun, University of Belgrade, Nemanjina 6, 11080 Zemun

 

Quality represents complex genetically determined traits. The numerous parameters are included in quality determination. In this work were presented analysis of sedimentation volume and loaf volume in 20 genetically different wheat cultivars. The gliadin and glutenin composition were analyzed by electrophoresis method. The relationships between Gli-1, Glu-1 alleles encoding gliadin and glutenin proteins and quality components were analyzed. The high sedimentation protein volume and high loaf volume values were established in cultivars (KG.56, KG.56S, Rodna and Tara) which carried Gli-Blb, Gli-Dlb, Glu-Dld. Also, positive correlation between GU-D2 and sedimentation volume as well as between Gli-B11 and loaf volume were established (Srbijanka, Ravnica, Jugoslavia, Balkan). The identified alleles at the Gli-1 and Glu-1 loci can use as markers of quality in wheat breeding. However, the analyzed components of technological quality are genetically determined, but not only by Gli-1 and Glu-1, than by other factors (Gli-2, Gli-3, ratio of gliadin/glutenin amount etc) which is necessary more study.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

DIFFERENCES BETWEEN HOMOZYGOUS AND HETEROZYGOUS WHEAT GENOTYPES REGARDING THEIR ANDROGENIC RESPONSE

 

Kondić Ankica i S. Šesek


Institute of Field and Vegetable Crops, Novi Sad

 

Androgenous and regeneration abilities of 10 heterozygous (F1 hybrids) and 13 homozygous (parents) winter wheat (Triticum aestivum L.) genotypes have been analyzed in in vitro culture.

Significant differences found among the genotypes with respect to the androgenous capacity, callus yield, frequencies of green and albino regenerants, indicate that these androgenetic components are genetically controlled.

Androgenous capacity in the homozygotes ranged from 0.6% (NS-0-694) to 20.3% (Zitnica), while in the heterozygotes from 3.9% (NS-O-694/Kosuta) to 34.7% (Szegedi-746/Zitnica). The average androgenous capacity of the F1 hybrids (13.8%) has been significantly higher than that of their parents (8.2%).

In the genotypes studied, callus yield ranged from 0.7% (Obrij) to 40% (Szegedi-746/Zitnica). On average, the heterozygotes had higher callus yields than the homozygotes.

Tree homozygous genotypes (Obrij, Szegedi-746 and NS-0-694) and one heterozygote (NS-O-694/Kosuta) have not regenerated green plants. The average frequency of green regenerants of the F1 hybrids (16.2%) has not been significantly different from that of the parents (14. 5%).

The heterozygotes had significantly higher average frequencies of albino plants (308%) than the homozygotes (22.7%).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INHERITANCE OF KERNEL NUMBER PER SPIKE IN CROSSING WHEAT GENOTYPES DIFFERING IN Rht GENES

 

Borislav Kobiljski1, Srbislav Denčić1, Marija Kraljević-Balalić2 i Rončević Petar1

 

1 Institute of Field and Vegetable Crops, Novi Sad

2 Faculty of Agriculture, Novi Sad

 

Recent results in research of Rht genes have shown that, besides plant height, they also affect other quantitative and qualitative traits in wheat. The aim of this work was to determine variability, mode of inheritance and gene effects of kernel number per spike in crossing wheat genotypes differing in Rht genes. The crosses were: Bankut 1205 (Rht 0) / Aobakomughi (Rht-Dlb), Bankut 1205 (Rht 0) / Sava (Rht 8), Siete Cerros (Rht-Blb) / Tom Thumb (Rht-Blc), Siete Cerros (Rht-Blb) / Ai-bian 1 (Rht-Dlc) and Sava (Rht 8) / Ai-bian 1 (Rht-Dlc).

Kernel number per spike varied in parents between 26.5 (Sava) and 36.1 (Siete Cerros), in F1 from 27.6 (Siete Cerros / Ai-bian 1) to 45.1 (Sava / Ai-bian 1) and in F2 from 27.6 (Bankut 1205 / Aobakomughi) to 40.0 (Siete Cerros / Tom Thumb). Significant positive heterotic effects have been determined in three crosses and significant negative effect in one cross (Siete Cerros / Ai-bian 1). No heterosis occured in the cross Siete Cerros / Tom Thumb. In the F, and F2 generations, kernel number per spike was inherited dominantly (partial dominance, full dominance or overdominance). The additive-dominance model showed that the inheritance of kernel number per spike was controlled mostly by the dominant genetic component (Bankut 1205 / Aobakomughi, Siete Cerros / Tom Thumb i Siete Cerros / Tom Thumb), or mostly by the additive component in two other crosses. Also, the presence of different epistatic gene effects has been determined in all crosses.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EVALUATION OF DISCRIMINATION ABILITY FOR MORPHOMETRIC LEAF PARAMETERS OF EASTERN COTTONWOOD

 

Branislav Kovačević


Poplar Research Institute, Faculty of Agriculture, Novi Sad

 

Eight measured and fifteen derived morphometric leaf parameters of one-year old rooted cuttings are examined for ability to discriminate 16 clones of eastern cottonwood (Populus deltoides) and four clones from hybrid combination P. deltoides x P. x euramericana and P. deltoides x ?. Discrimination loadings of first four canonical discrimination functions in the model with all 23 examined leaf parameters are higher with derived leaf parameters, especially with parameters that describe leaf blade shape. By forward and backward stepwise discrimination analysis eight-parameter models were formed. Except in one case, in bought models appeared same parameters. Four parameters in forward stepwise model and three parameters in backward stepwise model were measured parameters despite their low broad sense heritability. That could be explained by multicolinearity among derived parameters.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CHARACTERISTICS OF PERSPECTIVE LINES OF WINTER WHEAT OF CENTER FOR SMALL GRAINS IN KRAGUJEVAC

 

Miroslav Kuburović, Milanko Pavlović, Milivoje Milovanović, Veselinka Zečević

 

ARI SERBIA, Center for Small Grains Kragujevac, Save Kovacevica 31, Kragujevac 34000

 

The perspective lines of winter wheat of Center for Small Grains in Kragujevac and Standard cultivars Pobeda and Partizanka were investigated on several localities (Kragujevac, S. Mitrovica, Sombor and Pec). The eight lines: KG. 381, KG. 110/95, KG. 131/94, KG.3059-4/93T KG.8072, KG 14/1, KG. 1/1 i KG.19I/I were selected for analysis. In average, all lines on each localities stowed higher average value of grain, yield than check Pobeda cultivar, from 369 kg/ha to 1015 kg/ha. The lines have strong stern and resistance to lodging and height of stem, varied from 74 to 83 cm. These lines showed resistance to important pest and diseases of wheat as well high resistance to low temperature. Thousand grain mass varied in wheat lines from 37 g to 44 g, and hectoliter mass of grain varied from 75.7 kg to 83.3 kg. The analysis showed excellent value of quality parameters of flow and bread. Sedimentation value of some lines (KG.381) was on the level of cheek cultivar Pobeda. Properties of bread of some lines (KG.381, KG.110/95, KG.3059-4/93, KG.80721) was on the level of check cultivar Pobeda. Analyzed lines belong to Ai-B2 quality group (Pobeda belongs to A2). On the base of obtained results all lines submitted and included in micro trials of Federal Commission for cultivar Approvement of agricultural plant in 1998 year.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BREEDING ALFALFA GENOTYPES FOR INCREASED SEED YIELD

 

Lukić D1., Marija Kraljević-Balalić2

 

1 Institute of Field and Vegetable Crops, Novi Sad

2 Faculty of Agriculture, Novi Sad

 

In 1995 and 199, ten genotypes of blue alfalfa and 10 genotypes of hybrid alfalfa were analyzed for quantitative characteristics and seed yield components. The genotype H-40 had the largest number of stems with bolts. The blue alfalfa genotype S-8 arid the hybrid genotypes H-35 and H-34 stood out for the number of branches per plant - 57.2, 60.1 and 59.1 branches per plant, respectively. The blue alfalfa genotype S-9 and the hybrid alfalfa genotype H-35 had the highest average heights, 62.1 cm and 61.2 cm, respectively. High pollen fertility was exhibited by S-l, H-35 and H-34, 86.2%, 86.4% and 85.8%, respectively. Outstanding pod set per plant was found in S-9 and H-34, 1315 and 1295 pods per plant, respectively. They were followed by the genotypes H-33, H=35, etc. The genotype S-6 had the largest number of pollinated flowers per plant - 165.8. This genotype, which produced an average of 3.1 seeds per pod and the largest number of seeds per plant, 4760 was most productive, with 140.5 kg of seed per ha. High productivities were also exhibited by the genotypes H-36 and S-5, 4727 and 4644 seeds per plant, respectively, or 135.5 kg/ha. Blue alfalfa genotypes S-3 and S-2 and hybrid alfalfa genotypes H-35 and H-32 were also distinguished for high productivity.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC ANALYSIS OF THE GRAIN MASS PER PLANT IN BARLEY HYBRIDS

 

Milomirka Madić, Miroslav Kuburović i Aleksandar Paunović

 

Faculty of Agronomy, Cacak

 

Four divergent genotypes of barley (HVW-247, Partizan, NS-293 i Japanska 15) were chosen with the aim of analysing the inheritance mode and gene effect for grain mass per plant. By crossing of selected genotypes, F1 and F2 generations were obtained. The mode of inheritance was assessed on the basis of the significance of the differences in mean values of the hybrids in relation to parental average. The method of Jinks (1954) Hayman(1954) and Mather and Jinks (1971) was applied to establish the components of genetic variance and regression analysis. It was found that the variability of the trait under study was different, with variation coefficient found as the highest in the crosses HVW-247 x NS-293 (60%) and the lowest in the cross NS-293 x Jap. 15 (32%) The mode of inheritance of grain mass per plant seemed to be different depending on the cross combination, with partial dominance, dominance and overdominance exhibited in F1 and F2 generations. The value of additive component of the variance (D) was lower than the values H1 and H2 in both generations, indicating a stronger effect of dominant genes on the inheritance of grain mass. The average degree of dominance was higher than one (1.32) thus pointing at the overdominance in the inheritance of grain mass. This is confirmed by the regression line intersecting Wr axis below the point of origin. According to the way in which the points of scatter diagram were distributed along the regression line, we concluded that in the cultivar (NS/293) dominant genes prevailed, whereas in the cultivar HVW-247 recessive genes did.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STUDY OF SOME CHARACTERISTICS OF BARLEY VARIETIES EN F1 GENERATION

 

Dragoljub Maksimović1, Dušan Urošević1, Aleksandar Paunović2 1

 

1 Center for Small Grains, Kragujevac

2 Faculty of Agronomy, Cacak

 

Study of some characteristics in F1 generation in three combinations of Barley was presented in this paper.

Crosses between Barley cultivars (unresistant to low temperatures) varety pyramidatum with variety nudipyrami datum in F1 generation was studied. In F1 generation variety pyramidatum was completely dominant. This was obtained by chaff presence on F1 plants. F1 hybrides had the resistance to low temperatures as variety nudipyramidatum with kernels without chaff. Other characteristics of F1 hybrides: plant height spike height, general and productive tillering, vegetative period duration, rachis ankle number, length and width of second leaf under the ear, and length of the smallest-youngest leaf maesured before freezing had the same values as one of the parental varieties, were between parental values, had higher or lower values of both parental varieties which were different in only one gene who contrails chaff presence or abscence on kernels.

Crosses between Barley cultivars (resistant and unresistant to low temperatures) variety pallidum with variety nudipyramidatum was also studied in this paper. Variety pallidum was completely dominant in F1 generation. In F1 generation of pallidum variety the dominant characteristics were: resistance to low temperatures, plant height and ear length, rachis ankle number (same values as pallidum variety). But, F1 hybrides had higher general and productive tillering and second leaf under the ear length than both parental varieties. Characteristics: width of the second leaf under the ear, length of the smallest youngest leaf measured before freezing and vegetative period duration were between parental cultivar values of pallidum and nudipyramidatum variety.

Crosses between cultivars resistant to low temperatures (both belong to pallidum variety) was also studied.

F1 hybrides were resistant to low temperatures (-15 °C, duration 6 hours ) like more resistant parent. Other characteristics examined had the values like one of the parents, between parental values, higher or lower values than both parents of variety pallidum.

In winter Barley breeding the very important characteristic is resistance to low temperatures. In this study was pointed out presence of positive and complete significant (P0.05, P0.01) statistical correlation between characteristics resistance to low temperatures and ear height. But, between characteristics resistance to low temperatures, rachis ankle number and width of the second leaf under the ear was estimated negative and complete statistical significant (P0.05, P0.01) correlations. Very strong, positive and statistical significant correlation (P0.05, P0.01) was between characteristics resistance to low temperatures and second leaf under the ear.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

HERITABILITY OF RAPESEED (Brassica napus L.) YIELD COMPONENTS

 

Ana Marjanović-Jeromela, Radovan Marinković

Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad

 

In order to determine heritability in wide sense of rapeseed yield components, six genotypes originating from different regions were studied.

Experimental results were analyzed for plant height, height to first branch, stem diameter, branch number, leaf number, number of pods on branch, number of seeds in pod, seed yield per plant, mass of 1000 seeds and oil content. All yield components had high heritability values.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

LOCAL POPULATIONS AND SEMI-WILD TOMATO FORMS AS A SOURCE OF

GENETIC DIVERGENCE

 

Marković Z., Zdravković Jasmina, Damjanović M.

 

Agricultural Research Institute Srbija,Centre for Vegetable Crops Karadordeva 71, Smed. Palanka

 

We investigated the sample of 125 entirely different local populations and semi-wild tomato forms originating from the area of the former Yugoslavia. We tested both the plant and the fruit characteristics: plant height, internode length, number of flowers up to the first flower branch, earliness, fruit mass, number of locules per fruit, fruit shape, fruit colour, jointless gene, fruit firmness, radial and concentrically cracks, green back, fruit fasciation, blossom-end rot, dry matter content, p-carotene content, C- vitamin content, tolerance to diseases and pests, tolerance to high and low temperatures, length of shelf-life of biologically mature tomato fruits, etc. The investigation was carried out in the laboratory and in the open field applying the 1BPGR descriptor. The results showed that there was a strong genetic divergence for most of the investigated features, as well as the presence of the specific genes for a great number of characteristics favourable for selecting (i.e. earliness, fruit firmness, absence of cracks and fasciation, high content of vitamins, genetic resistance to diseases, pests and unfavourable weather conditions). This material could be highly significant and it could also be successfully used for the process of tomato selection.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF SOME QUALITATIVE CHARACTERS OF AUSTRIAN PINE HALF-SIB LINES IN A SEED ORCHARD ON JELOVA GORA

 

Milan Mataruga, Vasilije Isajev, Mirjana Šijačić-Nikolić

 

Faculty of Forestry, Belgrade

 

The study was carried out in Austrian pine seed orchard established in 1991 with seedlings from 40 half-sib lines, by the principle of metapopulation structure. Based on qualitative characters, we distinguished and set aside the phenotypes with gray needles, considerably shorter needles, irregular branching, as well as phenotypes that entered the reproductive phase during the study (seven-year old seedlings). The analysis of the contents of photosynthetically active substances (chlorophyll a and b, carotenoids) and needle anatomy showed a significant difference of tested phenotypes compared to the population average. The results are a significant contribution to the study of the magnitude of Austrian pine potential variability, as well as the assessment of metapopulation structure of seed orchard for multiannual gene pool testing, its conservation and long-term utilization.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

POSSIBLE USE OF MICROPROPAGATION OF DORMANT BUDS FROM ROOT HEAD OF SUGAR BEET IN RECURENT SELECTION

 

Mezei S., Čačić N., Nagl N., Kovačev L. i Sklenar P.

 

Naučni institut za ratarstvo i povrtarstvo, Maksima Gorkog 30, Novi Sad

 

The aim of research is to maintain and improve productivity of hybrid variety Crvenka mz , by recurrent selection on special combining abilities. After individual analysis of root weight and refraction, 106 genotypes was selected from self-sterile tetraploid population C-8173, which is the male component of the variety. Each genotype was planted on separate plot with CMS line A-0401, in order to obtain F1 generation. Micropropagation of dormant buds from root head is used to preserve selected genotypes in unchanged condition until the complete analysis of productive traits of F1 generation. Until now, explants from sterile seedlings were used in micropropagatiou of sugar beet, as well as explants taken from greenhouse or field. Sterilization of these explants, in order to eliminate bacterial and fungal infection, always presented a problem, especially for dormant buds, for which standard sterilization procedure is still not known. In the paper are presented results of nine sterilization methods of dormant buds taken from field. Sterilization with 0.3% BENLATE and ().7%NaOCl proved to be the best procedure and gave 47.1% of sterile plants.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BIOLOGICALLY-POMOLOGICAL CHARACTERISTICS OF BUSHES AND

TREES OF FOREST EUROPEAN FILBERT (C. avelana L) AND THE EUROPEAN

FILBERT (C. colurna L) IN EASTERN SERBIA

 

Rade Miletić1, Radoljub Petrović1, Milisav Mitrović2

 

Institut za istraživanja u poljoprivredi SRBIJA-Beograd

Centar za poljoprivredna i tehnološka istraživanja-Zaječar
Centar za voćarstvo i vinogradarstvo-Čačak

 

The forest European filbert and the European filbert are multiplied generatively. So that the populations are heterogeneous with bushes and trees of various biologic and pomological characteristics. Among great number of evaluated and analysed bushes and trees with the most favourable characteristics, certain ones have been chosen for further investigations in the same conditions. By planned multiplying and spreading of chosen types, in would be possible to provide more regular, better and fruitfulness of higher quality of both C. avelana L. and C. colurna L., particularly in supplying the basic material for production of biologically more valuable food. According to the results obtained from the sieve conditions, the fruits of chosen European filbert bushes and trees are characterized by great coarseness, mass, kernel content and oil content. The fruit coarseness of the chosen bushes of European filbert ranged from 20.7-15.6x16.6-13.2x13.7-11.6 mm (length, width, depth); the kernel coarseness ranged from 15.6-10.9x10.2-7.8x10.1-6.4 mm; the fruit mass ranged from 1.80 to 1.01 g, the kernel mass from 0.79 to 0.38 g, the kernel content from 46.2 to 27.9% and the oil content from 71.8 to 45.7%. The fruits of C. colurna L. are coarse with the dimensions 17.2-15.1x16.5-11.6x12.1-7.7 mm, and of the kernel 14.4-10.3x12.5-8.4x7.9-5.3 mm. The fruit mass range from 1.75 to 1.03 g, the kernel mass from 0.57 to 0.31 g, the kernel content from 40.8 to 31.1%, and the oil content from 74.4 to 63.2%. The appearance of the pest Balaninus is irrelevant; the amount of shrivelled grains is from 10.2 to 0.0%, that is, from 8.7 to 0.2%. The fruits are grouped, most often at the C. avelana L., from 1 to 4, the dominant from is 2 and 3 fruits together, and at the C. colurna L., from 1 to 10, the dominant from being from 5 to 7 fruits together.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

FENOLOGICAL REGULARITIES IN WALLNUT TREES POPULATION IN REGION OF TIMOCKA KRAJINA

 

Rade Miletić

 

Institut za istraživanja u poljoprivredi SRBIJA-Beograd, Centar za poljoprivredna i tehnološka istraživanja-Zaječar

 

Investigations of walnut trees population in the region of Timocka Krajina included the fenological characteristics, as well. In this study, the regularities among certain fenological characteristics are considered, such as; time of vegetation onset, blooming and amending time, time of vegetation competition. It has been analysed the relation of the influence of vegetation onset on the resistance to low wintry temperatures and the parasite inciter of the angular leaf spot (Isariopsis griseola). According to the comparative analysis of three-year average results referring to randomly chosen 1464 walnut trees in five sites in Timocka Krajina, numerous conclusions can be made. Earlier onset of vegetation and early, that is mean time of vegetation completion is the characteristic of 32.7, that is 38.5% of all walnut trees. The trees of earlier vegetation onset and early (29.22%) that is of mean time of amending (19.23%) dominate in the whole population. Among the early moving trees, the greatest number is of those with mean resistance to low wintry temperatures (50.0%), but weakly resistant ones, as well (15.37%>). Mean resistance (20.42%) is remarkable in the mean moving walnut trees. Low degree of sensitivity to the parasite, inciter of the angular leaf spot, is characteristic for the trees of later and mean late time of vegetation onset. However, the trees of earlier vegetation onset are more sensitive to the before mentioned pest occurrence.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE CLUSTER ANALYSIS OF MAIZE INBRED LINES ON THE BASIS OF POLYMORPHISM OF PROTEINS MARKER

 

Gordana Milojević, Snežana Mladenović Drinić, Goran Drinić i Kosana Konstantinov

 

Maize Research Institute, 11080 Zemun Polje-Belgrade

 

The method of genetic similarity assessment for maize inbred lines on the basis of embryo salt soluble proteins is proposed. Salt soluble proteins were extracted from dry embryo of maize inbred lines and electrophoretic separation were performed in 12.5%, polyakrilamide gels (PAGE). It was noticed the great polymorphism of embryo salt soluble proteins at their electrophoretic pattern, and that it is suitable for inbred line distinguishing. The protein pattern has confirmed the great differences among lines derived from different genetic sources and in a lower degree was observed for lines from similar sources. The indexes of similarity between analysed lines were calculated and cluster analysis according UPGMA was made. The cluster analysis has confirmed differentiation among lines and mat may be used for validation of pedigree records.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INHERITANCE OF GRAIN NUMBER PER SPIKE IN HEXAPLOID TRITICALE

 

Milivoje S. Milovanović1, VladanPešić2

 

1ARI'Serbia', Center for Small Grains - Kragujevac, Yugoslavia

2 Faculty of Agriculture Zemun - Belgrade, Yugoslavia

 

In this paper the inheritance of grain number per spike in hexaploid triticale was investigated. Studies were performed on primary spikes of parents, F1 and F2 progenies of a 5 x 5 complete diallel crossing. The results highlighted the prevalence of dominance and over-dominance in the expression of this trait at majority of combinations of F1 generation. Significant differences between direct and reciprocal crosses appeared in both generations and in generally with combinations of the most divergent parents. Analysis of variance for combining abilities demonstrated the existence of highly significant differences for general combining ability (GCA), specific combining ability (SCA) and effects of reciprocal crosses. GCA and SCA variations demonstrated similar values, which were for about three times higher than variation caused by reciprocal effects. The best SCA were expressed in generally in combinations of good x average and good x poor GCA of parents.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

RATIO OF POLLEN FUNCTIONAL ABILITY AND FRUIT SET DEGREE IN GRAPEVINE (Vitis sp.)

 

Momčilo Milutinović, Dragan Nikolić, Milica Fotrić i Vera Rakonjac


Faculty of Agriculture, Belgrade-Zemun

 

In this paper, pollen functional ability of cultivars Chardonnay, Smederevka and SV 12-375 that were used as a male parent for pollination of the cultivar Bagrina, were investigated. Bagrina is a grapevine cultivar with functionally female flower, and was used as a female parent in this paper. Besides controlled pollination, free pollination of the cultivar Bagrina was observed as well. Pollen functional ability was determined by acetocarmine coloring method and in vitro germinating method. Fertility degree was established based on fruit set number and fruit harvest number. All examined cultivars showed high percentage of colored pollen grains (over 75%). Satisfactory pollen germination in vitro was established in cultivars SV 12-375 (76.65%) and Smederevka (66.08%), while the cultivar Chardonnay had low pollen germination (20.88%). The largest fruit set number and fruit harvest number were obtained as a result of free pollination (51.43%; 36.94%). Considering examined crossing combination, the lowest fruit set number" and fruit harvest number had combination Bagrina x Chardonnay (29.32%; 15.93%). The largest fruit set number was obtained in crossing combination of Bagrina x SV 12-375 (39.10%), while the largest fruit harvest number had combination Bagrina x Smederevka (23.90%). Dependence between pollen functional ability of pollinators and fruit set degree of the cultivar Bagrina was determined. Cultivars SV 12-375 and Smederevka were good pollinators for the cultivar Bagrina.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COEFFICENT OF FRUITFULNESS OF APPLE VARIETIES AND HYBRIDS

 

Milutinović M.M.1, Milutinović D.M.2

 

1 PKB INI Agroekonomik, Padinska Skela

2 Faculty of Agriculture, Belgrade – Zemun

 

It was investigated introduced apple varieties (Shei seedling, Freedom, Liberty, Novamec and Nova izigro) resistant to cause of apple scab and hybrids (NJ 327246 and Ida 79) also resistant to Venturia inaequalis. The investigations were done in 1995 and 1996 at the locality PKB "Vocarske plantaze" in Bolec. Fruitfulness coefficient was established on the basis of percentage amount of setted and harvested fruits during mutual pollination, free pollination and selfpollination of investigated varieties. Fruitfulness coefficient of investigated apple genotypes is the highest with the variety Novamek (31.07%), followed by apple hybrids (NJ 327246 and Ida 79) with 19.85%, that is 14.76%. The lower fruitfulness coefficient percentage was the variety Nova izigro 6.38%.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INHERITANCE OF BERRY SKIN COLOR IN SEEDLINGS OF F1 GENERATION OF INTERSPECIES HYBRIDS IN GRAPEVINE

 

Milutinović. M., Žunić. P., Simić V.

Faculty of Agriculture Belgrade-Zemun

 

The berry skin color is important ampelographical characteristic, especially, for wine cultivars, because the color components are mostly in berry skin. The skin color is determined by O.I.V. Code N° 225 and it has marks from 1 to 7 (1 - green-yellow; 2 - rose; 3 - red; 4 - red-gray; 5 - dark-red-violet; 6 - blue-black; 7 - red-black). The seedlings of F1 generation produced by interspecies hybridization of the Vranac cultivar (wine cultivar with the blue-black skin color) and interspecies hybrid with the green-yellow skin color B-7-2 was observed. The seedlings obtained by reciprocal hybridization of the parents were observed as well. Obtained seedlings had all marks except number 4. The seedlings produced by hybridization of cultivars B-7-2 x Vranac mostly had red-black skin color (mark 7) - 53.22% of seedlings; mark 1 - 33.87% and rest of population had marks 3. 5. 6. None of seedlings with the white skin color were found in combination of cultivars Vranac x B-7-2. If marks 5, 6 and 7 describe black or dark berry skin color, than 75.95% of seedlings had dark skin color. Red skin color is represented with 24.04% of seedlings. The ratio between black and red skin color is 3:1.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

RACEMOSE WALNUT 10/95

 

M. Mitrović, S. Milenković

 

Fruit and Grape Research Centre, Cacak

 

Clonal selection of walnuts in Yugoslavia resulted in numerous walnut types with, different economic-biological properties, which thrive in our agroecological conditions both in trials and in large scale production.

The types which besides their pronounced cropping also have racemose inflorescences merit special attention. The paper presents the characteristics of the selection 10/95 - Lozanj named after the place at which it was selected. It is distinguished by an exceptional cropping potential (there are almost no individual fruits on the tree and raceme contains 8-14 nuts).

The tree is vigorous, vital, with a widely spreading crown. According to flowering type, it is protandrous. Flushing time occurs in the third decade of April. Fruits are medium sized (averaging 10.4 g) with kernel percentage 48.6%. They are attractive with light kernel and soft shell.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PHENOTYPIC AND GENETIC RELATIONSHIPS BETWEEN WHEAT QUALITY

 

Novica Mladenov, Novo Pržulj, Nikola Hristov, Veselinka Đurić

Institute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia

 

Knowing the dependence of technological quality on its various components and the interdependence of bread quality components is a major prerequisite for a successful application of proper selection methods in breeding for high technological quality- of bread. During 1994/95 - 1996/97 period (three years), a series of small-plot trials with 16 winter wheat varieties were conducted at three locations (Novi Sad, Indjija, and Sremska Mitrovica). Each location in each year was considered as a separate environment. Quality tests were performed for each cultivar in three replicates. The following bread-making components were analysed: hectoliter mass (HM), yield of flour (FY), protein content (PC), sedimentation test (SE). wet gluten (WG), loaf volume (LV) and crumb number (CN). Phenotypic (rph) and genotypic (rg) coefficients of correlation were calculated. Although in most cases the phenotypic and genetic correlations closely agreed, in some instances the difference was higher, indicating the importance of environmental effects in estimating the characters in question. Protein content, sedimentation, and wet gluten were positively correlated with the other indicators of technological quality. Positive and significant correlations were also found between hectolitre mass and crumb number (rph,=0,480; rg=0,613) and between loaf volume and crumb number (rph=0,872; rg=0,905). Negative and significant correlations were recorded between loaf volume and flour yield (rph=-0,311; rg=-0,329) as well as between crumb number and flour yield (rph=-0,291; rg=-0,326).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF MORPHOLOGICAL TRAITS OF MAIZE (Zea mays L.) INBRED LINES, CONSERVED IN YUGOSLAV GENEBANK FOR MAIZE

 

Jasmina Muminović, Dražen Jelovac, Gordana Radović

 

Maize Research Institute, Zemun Polje

 

The Genebank for Maize, within the Maize Research Institute. Zemun Polje. conserves, studies and conducts a breeding programme in a huge number of maize accessions of different genetic composition (local populations, synthetic populations, inbred lines, composites). Morphological traits of plant, cob and kernel of each accession, regardless their genetic composition, are being described using the international descriptor list for maize.

The study included maize inbred lines as they have an outstanding importance in breeding maize hybrids. Three sub-families of each inbred line in a group of inbred lines conserved in the Genebank for Maize were chosen. Data on highly heritable morphological traits were analysed by principal component analysis.

The objectives were to reveal the extent of variability that exists among sub¬families within an inbred line and to determine variability among different inbred lines.

Principal component analysis showed the presence of high variability in morphological traits among inbred lines and tended to group inbred lines of similar background together.

The study confirmed that maize germplasm, conserved at the Genebank for Maize, is highly variable. Tin's variability can be successfully utilised further in maize breeding, using the heterotic effect - a result of crossing two maize inbred lines differing in origin and expressing high level of combining ability.

In conclusion, for more precise survey of existing variability, characterisation on biochemical or molecular level appears to be necessary.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE POSSIBILITY OF USING PEPPER POPULATIONS IN THE AIM OF FURTHER BREEDING

 

Živka Bukić, Siniša Milutinović, Danica Mladenović


Institut za istraživanja u poljoprivredi SRBIJA - Beograd
Centar za poljoprivredna i tehnološka istraživanja - Zaječar

 

During the many years Center for agricultural and technological research in Zajecar works on collecting of domestic pepper populations. The genotypes with the best traits were separated and with be use for further breeding in order to getting new cultivars with more stability yield and more tolerant to extreme climatic conditions.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INFLUENCE FROM S1 RECURRENT SELECTION IN MAIZE SYNTHETIC NSB

 

Aleksandra Nastasić, M. Stojaković, G. Bekavac, Z. Petrović, N. Vasić

 

Institute of Field and Vegetable Crops, Novi Sad

 

Studied in this paper was the effectiveness of five cycles of Si recurrent selection for resistance to the causal agent of Fusarium graminearum rot in the synthetic maize population NSB. Using the Nested design (random model), independent trials were carried out for each of the four cycles of S1 progenies (C0, C1, C3, and C5). Genetic and phenotypic correlations, heritability, indicators of trait variability, linear regression, and genetic gain per cycle of S1 recurrent selection were determined.

After five cycles of S1 recurrent selection, we observed a significant rise in stem resistance to Fusarium graminearum. The largest increase was recorded in C5. Increasing resistance to this pathogen during the selection cycles was accompanied by increases in grain yield. Grain yield (b= 0.193*) had a significant positive and disease index (b= - 5.558*) a significant negative coefficient of linear regression. In C0 there was a significant negative strong correlation between disease index and grain yield, but in C5 no such correlation was found between the two traits.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GROUP AND INDIVIDUAL POLLEN VARIABILITY OF SOME 5-NEEDLE PINES IN FUNCTION OF IMPROVEMENT

 

Biljana Nikolić1,Vasilije Isajev2


1
PE Srbijasume. Institute of Forestry, Belgrade
2
University of Belgrade, Faculty of Forestry, Belgrade

 

We have three pine species of subsection Strobus in Serbia: Pinus – peuce, Griseb., Pinus strobus. L. and Pinus wallichiana, A.B. Jackson. On the basis of pollen grain dimensions and coefficient of shape of morphometric characteristics have been established. Physiological properties of freshen and frozen pollen (germination percentage and germination energy) were assessed by Kobel method. The results proved affirmative approach in the possibilities of using inter- and intraspecific hybridisation (as tree improvement methods). Meanwhile, flowering phenophasis between species are not simultaneous (Macedonian pine flushed one month later). Because of that using fresh pollen of Macedonian pine in interspecies hybridisation with Vaymouth and Himalayan pine is not possible. Using 1-year stored pollen of P. pence this problem might be overcomed. Strong individual variability in pollen quality and the possibility of long-term conservation of these species need selecting the best trees pollinators before hybridization.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC ANALISYS OF YOUNG SHOOT CHARACTERISTICS IN GRAPEVINE PROGENY OBTAINED BY CROSSING MUSCAT HAMBURG AND VILLARD BLANC CULTIVARS

 

Dragan Nikolić

Faculty of Agriculture, Belgrade-Zemun

 

For ampelographic description and determination of grapevine cultivars, lots of morphological characteristics have been used. Among 21 characteristics that are investigated for a gene bank, there are 5 characteristics for young shoot. Those are: form of young shoot tip. anthocyanin coloration intensity of young shoot tip, prostrate hairs density of young shoot tip, erect hairs density on the nodes of shoot and tendrils distribution on the shoot. The variability and the mode of inheritance of cited characteristics have been investigated in 90 seedlings of F1 generation from the crossing combination of Muscat Hamburg x Villard Blanc. Categorization of examined characteristics has been done using OIV codes, and determination of the mode of inheritance has been done using χ2 test. Obtained results showed that in hybrid progeny the majority of examined characteristics had substainal variability. For form of young shoot tip and prostrate hairs density of young shoot tip. monogenic inheritance has been established. Exception to monogenic inheritance, anthocyanin coloration intensity of young shoot tip and erect hairs density on the nodes of shoot has been established. The mode of inheritance of tendrils distribution on die shoot, could not be determined because all of hybrid seedlings and their parents had discontinuous tendrils distribution on the shoot.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC AND PHENOTYPIC CORRELATION OF PHYSIOLOGICAL AND GROWTH PARAMETERS OF POPLAR CLONES

 

Saša Orlović1, Borivoj Krstić2, Bojana Klašnja1

 

1 Agricultural Faculty Novi Sad, Poplar Research Institute, Antona Cehova 13, 21000 Novi Sad. Yugoslavia, E-mail: sasao@polj.ns.ac.yu

2 Faculty of Naturally Sciences, Institute of Biology, Trg D. Obradovica 5, 21000 Novi Sad, Yugoslavia

 

This paper presents the results of the research of physiological characters of rooted cuttings of eight black poplar clones (4 Popuhis x euramericana and 4 Populus deltoides) in three field experiments on different soil types (humofluvisol, fluvisol f. loamy and fluvisol f. sandy). Physiological characters are net photosynthesis, dark respiration and leaf area. At the end of the vegetation period, the main plant growth elements were measured: diameter, height and biomass. The results of the research physiological processes of poplar clones showed a high interclonal variability of most elements and processes of species under study. Statistically significant differences between clones and insignificant between repetitions, medium and high coefficients of heritability in a broad sense, indicate that the majority of study characters are controlled by genetic factors which result in considerable specificities of some clones. The statistically significant interaction clone x experiment, in the greatest number of characters, indicates the different reactions of clones to the site, i.e. soil type. Also the rank of the clones in three experiments was not the same, consequently the interaction clone x environment existed in all the characters under study. The quotient of variance genotype x environment and variance of genotype, for the dark respiration, and rooted-cutting diameters and heights was higher than 0.5, and therefore the interaction genotype x environment must be considered during selection. A strong genetic correlation with the elements of growth and biomass was shown especially by leaf area. The results indicate that it could be possible to construct the hybrids with desirable level of physiological processes by which the effects of hybridization can be enhanced.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY OF BLACK WALNUT (Juglans nigra L.) PHYSIOLOGICAL PARAMETERS AND GROWTH ELEMENTS IN THE JUVENILE PHASE

 

Saša Orlović, Zagorka Petrov, Zoran Galić, Leopold Poljaković Pajnik

 

Agricultural Faculty, Novi Sad, Poplar Research Institute Antona Cehova 13, 21000 Novi Sad, Yugoslavia. E mail: sassao@pojns.ac.yu

 

Black walnut (Juglans nigra L.) was successfully introduced to our country. It is characterized by fast growth, good quality and economically valuable wood,, as well' as with low susceptibility to pests and diseases. Black walnut plantations are established on alluvial soils along lowland rivers. In Yugoslavia to date tow superior populations of this, species have been designated seed orchards, which is a base material for breeding. This paper presents the results of the study of variability of physiological parameters and elements of growth of one-year-old black walnut progeny from a registered seed orchard (reg. no. S 02.12.02.01) in a field test. The study physiological parameters: stomatal length and width, net photosynthesis respiration and the content of a and b chlorophyll and growth parameters: plant diameters and height. The results point to a very pronounced intra-population variability of all study parameters, as well as statistically very significant differences between the genotypes regarding net photosynthesis, respiration and the content of chlorophyll. A high correlation between the content of a and b chlorophyll and plant diameter and height was recorded.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STUDY OF INDIVIDUAL VARIABILITY OF HORSE CHESTNUT (AESCULUS HIPPOCASTANUM L.) IN THE AIM OF IMPROVED SECIES SELECTION

 

Mirjana Ocokoljić1, Vasilije Isajev1, Nada Kovačević2

 

1 Faculty of Forestry, Belgrade

2 Faculty of Pharmacy, Belgrade

 

Comparative morphological and biometric analysis of the seeds of 15 horse chestnut genotypes was performed in the aim of quantitative analysis of three groups of physiologically active seed constituents (escine, tannins, fatty oil and fatty acids). The assessment of the character of variability and the study of characteristics was based on the analysis of variance, cluster analysis and the coefficients of variation, as it can be considered that they reflect satisfactorily the hereditary potential of the selected individuals. The significant differences between test trees, as well as insignificant differences between the replicates, indicate that study properties are in the function of tree genotypes, and partly, some properties are under environmental impact.

The results point to the fact that the study of variability of test trees is of high significance for their selection and the establishment of good-quality horse chestnut plantations. By the selection of superior genotypes and by their propagation, the varieties can be synthetized that will, thanks to a high degree of general adaptability, ensure a proportionally high productivity of individual metabolites.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE FRAMEWORK OF VARIANTS OF HAPLOID CHROMOSOME COMPLEMENT PRODUCING A FINITE NUMBER OF PURE LINES

 

Boro P. Pavlović

 

Institute of Forestry, KnezaViseslava 3, Belgrade, Yugoslavia

 

A species typically consists of individuals with the same number of chromosomes in the chromosome set, or with the same number in the individuals of the same sex. The deviation from this characteristic of a species is the base of species evolution changes. Genetic variation of individuals intra species is contained in the variants of chromosome complements. A chromosome variant is characterized by a series of alleles. The change of allele representation in any gene locus leads to another chromosome variant. Each chromosome is represented by one variant in the haploid cell. Diploid cell can contain one or two variants of each chromosome. Depending on the state of the population, the number of variants of each chromosome in the chromosome complements can be N to 2N (N is number of individuals). In the classical experiments with bean, Johannsen obtained 19 pure lines. It is a question how many potential source states of chromosome set variants were there, if no source chromosome variants were lost, if the complete genetic analysis was performed. The solution of the problem is in the forms of the calculation of variants of haploid (V) and diploid (W) chromosome complements based on chromosome variants (v) and the number of chromosomes in the haploid chromosome complement (n): V=v1v2v3....vj....vn; W=V2. A possible number of pure lines is equal to the number of V variants. Invariant chromosomes in the chromosome complement have no consequence on the number of pure lines. The presented analysis of source sets (resulting in 1 to 127 pure lines) was discussed. The prime number of pure lines (such as 19), in the complete genetic analysis, is related to the source set of individuals, which is characterized by the variants of only one chromosome.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

VARIABILITY AND HERITABILITY OF ONION BULBS DIAMETER

 

Pavlović Nenad, Sušić Zoran, Zdravković Jasmina, Marković Živoslav, Stevanović Dušan

 

Agricultural Research Institute "Srbija", Center for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, e-mail: cfvcsp@,eunet.YU

 

In order to investigate the variability of bulb diameter, the trial was set up on an experimental plot of the Centre for Vegetable Crops in Smederevska Palanka during 1997 and 1998. The trial was performed by applying the method of random block system in five replications. Ten varieties of different geographical origins were included as the research material. The recorded values of the investigated features were determined by applying the variance analysis of a two-factorial trial - model 2 (Hadzivukovic, 1991). The components of the phenotype variance, genotype and phenotype coefficient of variation and heritability in broad sense were estimated according to Singh and Chaudhary (1976).

Significant variability was recorded for diameter bulbs in both years of investigation. For this parameter, genotype variance was greater than the environmental. Phenotype coefficient of variation (PCV) was greater than genotype coefficient of variation (GCV). High heritability confirmed that the genotype variability was stronger in the overall phenotype variability.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

PUTTING PHYSIOLOGY ON THE MAP - TESTING RELATIONSHIPS BETWEEN DROUGHT RESISTANCE TRAITS USING MOLECULAR MAPS

 

Sofija Pekić1, Vesna Lazić-Jančić2, Violeta Anđelković2, M Ivanović2, Zora Dajić1, Evonne Waterman3, H Rahman3, A Steed3 i SA Quarrie3

 

1 Faculty of Agriculture, Belgrade, Yugoslavia

2 Maize Research Institute "Zemun Polje", Yugoslavia

3 John Innes Centre, Norwich, UK

 

The aim of plant physiology is to understand how plants work: to establish the inter-relationships between processes that the plant integrates, from a single gene to the complex phenotype of a whole plant. The traditional approach has been correlation analysis of changes in the process and the observed phenotype. However, causal effects are difficult to prove in this way. The development of molecular marker systems, such as restriction fragment length polymorphisms (RFLPs) and microsatellites (simple sequence repeats, SSRs), to generate detailed genetic maps of any species provides physiologists with new opportunities to test the inter-relationships amongst processes and to identify those that are important in determining a particular phenotype. A range of statistical techniques is available to locate major genes regulating a particular trait or process (quantitative trait locus. QTL, analysis) and causal relationships between traits can be tested by looking for coincidence of QTLs for the traits.

We are using molecular maps of maize to identify the QTLs for drought responses and to identify those traits that are important in determining drought resistance, defined as yield under droughted field conditions. Our initial studies on drought resistance traits focused on leaf abscisic acid (ABA) content and the results of QTL analysis of leaf ABA content and yield under droughted field conditions are presented in an accompanying poster (Pekic et al.). Essentially, we used QTL analysis to show that, despite the reports of ABA having many effects on plant physiology, growth and development, there was no coincidence of QTLs for leaf ABA content and yield under droughted conditions.

We are now extending this work to study a wide range of physiological, morphological and biochemical traits that may be important in determining drought resistance in a maize population derived from a cross between a susceptible inbred line (B73) and a more resistant line (DTP79). Traits expressed in mature plants growing under droughted field conditions are being compared with traits of younger plants droughted under laboratory' conditions by testing coincidence of QTLs for the traits.

In a glasshouse experiment where plants were droughted over several weeks in soil columns, highly significant QTLs for leaf sap osmotic potential and soluble carbohydrate content (assessed by refractometry) were found coincident on chromosome 1. The same region of chromosome 1 also carried a gene(s) determining yield under droughted field conditions. QTL analysis of other aspects of plant growth and physiology will also be presented to illustrate how tliis technique can provide important information for physiologists on associations amongst traits.

We are grateful to the Royal Society, European Commission and Commonwealth Awards Division for providing financial support for this collaborative research.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

THE ROLE OF ABA IN DROUGHT RESISTANCE: PHYSIOLOGICAL AND GENETICAL ANALYSIS IN MAIZE

 

Sofija Pekić1, Lora Ljubojević1, Violeta Anđelković2, M Ivanović2, Vesna Lazić-Jančić2, A. Steed3 i SA Quarrie3

 

1 Faculty of Agriculture, Belgrade, Yugoslavia

2 Maize Research Institute "Zemun Polje", Yugoslavia

3 John Innes Centre, Norwich, UK

 

For several years, we have been studying the role of abscisic acid (ABA) in mediating the responses of maize to drought stress using a range of physiological, genetical and molecular marker techniques. Our initial work focused on the preparation of maize genotypes, by crossing and selection, that had contrasting capacities to accumulate ABA in their leaves and then studying the consequences of the selection procedure on a number of physiological and agronomic characteristics related to drought resistance. Both inbred lines and synthetic populations differing in leaf ABA content under field conditions were created for this work.

Significant differences amongst the inbred lines derived from the cross F-2 x Poljl 7 were found for the majority of characteristics tested (leaf water potential stomatal conductance, plant height, flowering time, etc) as well as yield under both draughted and irrigated field trials. However, the variation in these traits was not associated with variation in leaf ABA content. Comparison of the high- and low-ABA synthetic populations under rainfed conditions also showed no significant association of leaf ABA content with yield.

The same cross (F-2 x Poljl7) was also used to create a mapping population of 81 FJJ families which was mapped with around 100 restriction fragment length polymorphic (RFLP) markers. The families were trialled in the field and a number of physiological, morphological and agronomic characters, including leaf ABA content and yield, were analysed for quantitative trait loci (QTLs). There was no coincidence between QTLs for yield and leaf ABA content, showing no significant effect of leaf ABA content on drought resistance and confirming the earlier work with inbred lines and synthetic populations. Nevertheless, there were associations between QTLs for yield and anthesis-silking interval and the number of nodal roots at harvest, showing that these traits may be important in determining yield under draughted conditions.

Thus, we have shown that both classical and molecular marker methods provided the same conclusions about the role of ABA in these draughted maize plants. However, the molecular marker methods are more powerful in identifying in a relatively short period of time the traits that may be important in determining drought resistance as well as the location of genes regulating those traits.

We are grateful to the Royal Society and British Council for providing financial support for this research.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

COMBINING ABILITIES AND MODE OF INHERITANCE OF YIELD AND 100-GRAIN MASS IN MAIZE (Zea mays L.)

 

Petrović, Z.1, J.Bocanski2, Nastasić. Aleksandra1, G. Bekavac1, N. Vasić1

 

1 Scientific Institute of Field and Vegetable Crops, Novi Sad

2 Faculty of Agriculture and Institute of Field and Vegetable Crops

 

Using the line x tester analysis (Singh and Choudhary, 1976), we studied the mode of inheritance of grain yield and 100-grain mass of seven female lines, three tester lines, and 21 hybrids of maize.

The results of the study have shown that dominant genes play the most important role in the inheritance of 100-grain mass, and grain yield per plant in this crop species. Superdominance and heterosis were recorded in both characters under investigation. In most cases, the combination of lines with significantly negative and significantly positive general combining ability GCA values produced hybrids with a significantly positive specific combining ability SCA. Hybrids having a good SCA for 100-grain mass also had a significant SCA for grain yield per plant.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ESTIMATES OF GENETIC EFFECTS OF PRODUCTIVE TILLERING IN SPRING WHEAT

 

Novo Pržulj1, Novica Mladenov1, Miroslav Bogdanović2

 

1 Institute of Field and Vegetable Crops, Novi Sad
2
Faculty of Agriculture, Srpsko Sarajevo, Republic of Srpska

 

Grain yield of wheat depends on spike number per unit area, grains number per spike and grain weight. Some researches have shown that spikes number per unit area is often the yield components with which grain yield is most closely associated. The objectives of this study were to (i) estimate the mode of inheritance, (ii) gene effects and (iii) heritability of productive tillering in spring wheat. For mat purpose the next crosses between varieties with different productive tillering were made: low x low (Sonalika x Bobwhite, Lelija x Bobwhite) low x medium (Sonalika x Glennson 81. Lelija x Mitacore Vesa x Radusa) and low x high (Buckbuck x Dugoklasa). Additive-dominance (AD) model was used to estimate midparent (m), additive (d) and dominance (h) gene effects. The adequacy of the AD model was determined by the ABC scaling tests and the joint scaling test. When the AD model failed to fit the data a six-parameter model was fitted. Heritability was estimated from a generation variance analysis.

The mode of inheritance in F1's was overdominant and dominant for higher tillering as well as intermediate, while F2's were mainly intermediate. Based on the significance of the individual tests and the joint scaling test it can be concluded that AD model was not adequate for any of the six crosses and that nonallelic interactions had important part in the productive tillering inheritance. Thee types of digenetic interactions (additive x additive, additive x dominance and dominance x dominance) were present in four of six crosses. Interactions mainly had negative sign and decreased productive tillering. Heritability estimates was about 35% in narrow and varied from 48 to 63% in broad sense.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STRUCTURAL CHANGES OF WHEAT POPULATIONS BY BULK SELECTION

I. Mean values and correlation among traits

 

S. Prodanović, G. Šurlan-Momirović. D. Perović, I. Stančić, Z. Nikolić i Z. Veselinović

 

Faculty of Agriculture, Belgrade-Zemun

 

Ten populations of F3 and F4 wheat hybrids and their parents has been investigated at two locations (Indjija and Pec). Bulk method of selection has been used to produce these populations. Six characters of materials were analyzed: plant height, spike length, spikelet number per spike, grain number per spike, grain mass per spike and grain yield. For all characters, exept for grain number and mass per spike, increasing of mean values (in relation to middle parents - MP values) has been found in F4 generation. This fact shows the high efficiency of bulk selection in wheat breeding. All yield components have been positively correlated with grain yield in both generations, but stronger in F3 than in F4 generation (R = 0.94 in F3 and R = 0.83 in F4). The grain mass per spike shown the highest influence on grain yield (r = 0.90 in F3 and r = 0.73 in F4), and this elementary trait was in the strongest relation with grain number per spike (r = 0.92 in F3 and r = 0.87 in F4).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

STRUCTURAL CHANGES OF WHEAT POPULATIONS BY BULK SELECTION

II. Analysis of homology in variation of traits

 

S. Prodanović, G. Šurlan-Momirović, B. Jovanović i M. Menkovska

 

Faculty of Agriculture, Belgrade-Zemun

 

The high homology among changes of grain yield, plant height and spike length, was observed by analysing the variability of mean values of traits in 10 wheat hybrid populations and their parents, growing at two locations. However, how this homology was not supported by significant correlation and how there was not homology in variation between grain yield and two characters mat showed the highest correlation with grain yield (grains number and mass per spike), it was concluded mat bulk selection affects changes in the structure of populations. One hypotesis has been established that these changes include follows aspects: 1) increasing of number of spikes per area unit, 2) better distribution of spikes in relation to plant height and 3) equalizing of grain mass between primaries and secondaries spikes. By these possibles changes in population structure could be explained the decrease of variability length among all characters from F3 to F4 generation, when their mean values at two locations were compared.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC AND PHENOTYPIC CORRELATION AND PATH ANALYSIS IN PEACH

 

Vera Rakonjac

 

Faculty of Agriculture, University of Belgrade, 11080 Belgrade-Zemun, Yugoslavia

 

This investigation was designed to study the inter-relation ship of some agrobiological characteristics (flowering time, ripening time, fruit set, fruit harvest, fruit weight and stone weight) and the correlations with yield. Yield was significant or very significant genetically correlated with fruit set (0.377*), fruit harvest (0.388*), fruit weight (0.851**), and stone weight (0.481**). There was very significant phenotypic correlation between yield and fruit set (0.576**), as well as between yield and fruit weight (0.556**). Genetic path coefficient analysis indicated a significant direct effect of fruit set on yield. Phenotypic path coefficient analysis showed that beside fruit set, fruit weight had significant direct effect on yield. The indirect effect of fruit harvest via fruit set and flowering time via fruit set was higher than the direct effect.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECT OF PEMF ON GRAIN NUMBER AND GRAIN MASS PER SPIKE IN SPRING WHEAT

 

Petar Rončević1, Nikola Hristov1 i Marija Kraljević-Balalić2

 

1 Institute of Field and Vegetable Crops, Novi Sad

2 Faculty of Agriculture, Novi Sad

 

The effect of pulsating electromagnetic field (PEMF) on grain number and grain mass per spike has been studied in 10 spring wheat genotypes. The studied genotypes come from different centers of origin. In the period 1995 to 1998, the experimental material was analysed applying the following method: water-soaked seeds of the 10 genotypes were once directly exposed to PEMF for 30, 60 and 90 minutes (frequency 72 Hz, voltage 1 mT). After treatment, the seeds were planted in pots and, at maturity, spikes were analyzed for grain number and grain mass. For the most genotypes the stimulation caused increases in the number of grains and grain mass per spike. The next step was to establish the expression of the observed changes in subsequent generations. The seed obtained in pots (EM0) was planted in the field for the next three seasons to produce EM1, EM2 and EM3 generations. The changes caused by the direct exposure to PEMF were evident in the subsequent generations, especially in the M3 generation. It seems that a single exposure of spring wheat seed to PEMF leads to permanent changes.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CORRELATIONS AND PATH-COEFFICIENTS ANALYSIS IN SUGAR BEETS ROOT TRAITS

 

Pavle Sklenar, Lazar Kovačev, Nikola Cačić, Snežana Mezei i Nevena Nagl

 

Institute of Field and Vegetable Crops, M. Gorkog 30, Novi Sad, Yugoslavia

 

The aim of sugar beet growing is to maximize the yield of crystal sugar per unit area. Investigated in the present paper were core correlations and direct and indirect effects of root weight, root volume, sugar content and dry matter content on crystal sugar yield. Two sugar beet populations, A8415 and 1102-5-8, were examined. These populations are monogerm maintainers of citoplasmical-nuclear male sterility (O-type) that differ significantly with respect to the aforementioned traits. In population A 8415 highly significant core correlations were found between the studied traits and crystal sugar yield, while sugar content, dry matter content and dry matter yield had a very significant direct effect on crystal sugar yield. The coefficient of multiple determination had a value of r2=0.99 and the residual effect was at R=0.04. In population 1102-5-8 core correlation were very significant only between root weight, root volume, dry matter yield and crystal sugar yield. These traits also had a highly significant direct effect on crystal sugar yield. The coefficient of multiple determination had a value of r2=0.90 and the residual effect was at R=0.31. This type of information could help sugar beet breeders improve the efficiency of their breeding process.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

EFFECTS OF SISTER-LINE CROSS ON GRAIN YIELD INCREASE IN SOYBEAN LINES WITH REDUCED ACTIVITY OF TRYPSIN INHIBITOR

 

Mirjana Srebrić

 

Maize Research Institute, ZemunPolje, S. Bajica 1,11080 Belgrade-Zemun

 

The cross of an adapted variety Kador to the variety Kunitz, as a donor of favourable traits - a decreased content of trypsin inhibitor in grain, was conducted with the aim to develop more yielding lines with modified chemical content of grain.

The progeny of 48 highest yielding F2 plants were tested and then two top yielding F3 lines were selected. Forty individual plants of the highest yielding line (L38) were crossed to 40 plants of the line ranking second (L30). Grain yield of progenies of female components and of the combination L38 x L30 was tested. Increased grain yield was found in approximately half of the progenies from the sister-line cross L38 x L30 in relation to non-crossed female components. Such developed material will be a starting point for selection of superior soybean lines with decreased content of trypsin inhibitor in grain.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ACCUMULATION OF FAVOURABLE ALLELES IN TWO SYNTHETIC POPULATIONS OF MAIZE (Zea mays L.)

 

Stanković G., Trifunović B.V., Trifunović V.

 

Maize Research Institute, Zemun Polje, 11080 Belgrade-Zemun, Yugoslavia

 

The important objective in plant breeding is to develop a source material of high quality (local populations, synthetic populations, F2 populations, etc.) for further selection. At the beginning of the initial population development, a special attention should be paid to genetic variability (narrow and broad genetic base), as well as to the method of further selection. The aim is to derive a population with a high frequency of favourable alleles controlling yield and other traits of agronomic importance. The incorporation of exotic germplasm into local and/or adapted populations provides the enhancement of genetic variability and the increase of frequency of favourable alleles. Starting from studies of Trifunovic et al. (1994a, 1994b) our intention was to find out genetic progress in two synthetic maize populations and determine whether favourable alleles controlling yield and other traits of agronomic importance, accumulated upon the change of the selection method. After three cycles of combined selection, two maize synthetic populations (Syn MRI ZP -narrow genetic base and Syn MRI ZP/USA - broad genetic base) out of the three used in the experiment, were included into inbred tester recurrent selection. Both populations, after one cycle of selection, obtained accumulation of favourable alleles controlling grain yield (Syn MRI ZP - 7.452 t ha-1, i.e. Syn MRI ZP/USA - 7.466 t ha-1), while moisture percentage remained at the target level. The yield level of Syn FS (8.248 t ha-1), derived by crossing of the previous two synthetics, point out that these synthetics maintained their initial divergence. Yield of test crosses (to inbred of Lancaster background) of all the three populations indicates that Syn FS of Syn MRI ZP/USA population accumulated the greatest number of favourable alleles. The percentage of lodged and broken plants is significantly lowered in Syn MRI ZP and Syn MRI ZP/USA in .relation to previous cycles. It can be concluded that these three developed populations are a good initial material for long-term selection programmes and that the chosen method is a proper one for accumulation of favourable alleles controlling yield and other properties of agronomic importance.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

HETEROSIS FOR AGRONOMIC TRAITS OF BROOMCORN

 

Sikora V.

Institute of Field and Vegetable Crops, Novi Sad

 

Six cytoplasmic-genetic male sterile lines have been crosses with six fertility restorer lines of broomcorn by line x tester analysis.

For the components of plant height, the most significant heterosis compared to better parent has been observed for total plant height (16.7 %). The rest of the plant height components expressed less heterozis (peduncle lenght 8,1 %, flag leaf sheat 5.8 %, panicle length 2.0 % and fibber length 1.9 %). Negative heterosis has been measured for panicle exertion (-157.4 %). For the panicle weight components, the heterosis was positive for the weight of untreshed panicle (28.3 %), as well as for seed weight per panicle (23.6 %) and weight of the treshed panicle (14.3 %). The ratio between the treshed as compared to untreshed panicle showed negative heterosis value (-4.5 %). For the number and fineness of fibres medium level of positive heterosis has been obtained (5.7 % and 5.2 %).

Based on the results from this study, further activity aimed at commercial exploitation of heterosis, i.e. breeding of hybrid broomcorn varieties has been proposed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

SELECTING FEATURES FOR ESTIMATING GENETIC DIVERGENCE OF TOMATO GENOTYPES (Lycopersicum esculentum Mill.)

 

Sušić Zoran1, Zdravković Jasmina1, Pavlović Nenad1, Prodanović Slaven2

 

1 Agricultural Research Institute "Srbija", Center for Vegetable Crops, Karadjordjeva 71,

11420 Smederevska Palanka, cfvcsp@eunet.YU

2 Agricultural Faculty, Nemanjina 6, 11080 Zemun

 

On the basis of the six tomato yield components (fruit length and width, pericarp thickness, number of locules per fruit, number of fruits per plant, and fruit mass), seven tomato genotypes were grouped into five clusters by applying the method of hierarchical cluster analysis. The values of the specific combining abilities (SCA) and heterosis effects in 21 tomato hybrids of F, generation obtained by diallel crossing of seven parental genotypes testified that the dendogram of phenotypic differences was obtained on the basis of these features. It also showed the present differences between the genotypes. It was concluded that the good hybrids with high SCA values and high heterosis effects were obtained by crossing the divergent genotypes from different clusters. It was in conformity with the fact that the favourable gene recombining abilities and high heterosis effects were obtained by crossing the divergent parents but not the ones in close relationship. Since this method was successfully applied and tested on seven genotypes, it could also be applied for the characterisation and classification of the entire tomato gennplasm collection. The genotypes characterised by a high degree of genetic divergence should be sorted out from the collection mat was classified in such a manner (from different clusters). In this way, the process of tomato selection would be greatly facilitated.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

AMINO ACIDS IN THE FRESH FRUIT OF THE PARENTAL RASPBERRY CULTIVARS AND PROMISING SELECTIONS (Rubus idaeus L.)

 

Žarko V. Tešović, Slađana A. Nidžović, Milan M. Lukić

 

Agricultural Research Institute "Serbia" Fruit and Grape Research Centre, Cacak

 

Over three-year period, qualitative and quantitative analyses was employed to determine the content of 17 amino acids in raspberry fruits of four parental cultivars: Gradina. Krupna Dvoroda, Mailing Exploit and Willamette and of nine promising selections: 1/2 and 1/4 (Willamette x M. Exploit), 1/9, 1/11 and 1/12 (Willamette x Gradina) and 11/11. 11/12, 11/14 and I1I/5 (Gradina x Krupna Dvoroda). The content of following amino acids was determined: alanine, arginine, aspartic acid, cysteine + cystine, phenylalanine. glycine, glutamic acid, histidine, isoleucine, lysine, leucine, methionine, proline, serine, tyrosine, threonine and valine. Of these 17 amino acids, 7 are essential: arginine. isoleucine, leucine. lysine, methionine, threonine and valine. The average content of all the evaluated amino acids in the fruit of all the genotypes ranges from 310 μM (methionine) to 5527 μM (glutamic acid).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BIOLOGICAL PROPERTIES OF PROMISING APPLE SELECTIONS

 

Žarko V. Tešović, Sladana A. Nidžović, Milan M. Lukić

 

Fruit and Grape Research Centre, Čačak

 

Commercial apple production has strict requirements concerning the choice of cultivars for growing: excellent fruit flavour, attractive skin colour, spur, pendulous or columnar growth habit, winter frost hardiness, resistance to the casual agents of major diseases, which implies minimal pesticide use in the production of quality fruits and long shelf life without physiological diseases. Since the existing apple cultivars do not meet the mentioned requirements, the solution is in breeding new apple cultivars with desired economic-biological characteristics. Long-term work carried out in Fruit and Grape Research Centre, Čačak resulted in a great number of promising selections. This paper presents the economic-biological properties of four of them: J/12/6 (Golden Delicious x Cox's Orange Pippin), J/11/20 (Prima x Melrose), J/12/12 (Prima x Melrose) and J/12/35/90 (Prima x Melrose).

Selection J/12/6 belongs to the group of autumn apple cultivars, whereas the following selections J/11/20, J/12/12 and J/12/35/90 are winter apples. The latter are resistant to the two major apple diseases: mildew and apple scab.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BREEDING DWARFING APPLE GENOTYPES

 

Žarko V. Tešović, Slađana A. Nidžović, Milan M. Lukić

 

Fruit and Grape Research Centre, Čačak

 

In terms of modern technology of growing and fruit production, vigour is a highly important apple characteristic. All apple growers prefer a dwarfing rootstock, since it enables grafting onto vigorous rootstocks requiring no support, which reduces the costs of establishing plantings even up to 40%. Smaller growth habit enables the planting of a great number of nursery trees per ha. Also, it facilitates pruning, fruit protection and harvest. Thus, the work on developing dwarfing apple genotypes has been intensively pursued at Fruit and Grape Research Centre, Cacak over last 10 years.

Planned hybridization within three parental combinations resulted in 787 hybrid seedlings, as follows: Cox's Orange Pippin x Cadel - 195 seedlings; Cox's Orange Pippin x Idared - 337 seedlings and Jonathan x selection TSR13T84 - 255 seedlings. All the seedlings were grown under the same conditions in ceramic plots and substrate for raising apple seedlings. At the end of the first growing season, the height of seedlings was measured and they were accordingly classified into seven groups. The greatest number of seedlings from the first and second combinations (36.4%, i.e. 36.7%) belonged to the second group (height - from 5.0 to 10.0 cm), whereas in the third combination, the largest number of seedlings (26.6%) belonged to the third group (height - from 10.0 to 15. 0 cm). These data indicate that an adequate choice of parental pairs can lead to appropriate vigour, i. e. dwarfing of apple seedlings.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

INBREEDING DEPRESSION OF PRODUCTIVE TRAITS IN THREE SPECIES OF THE GENUS Agrostis L.

 

Zorica Tomić1, Sokolović, D.1 i Gordana Šurlan-Momirović2

 

1 Agricultural Research Institute ''Serbia", Center for Forage Crops, Krusevac

2 Faculty of Agriculture, Belgrade-Zemun

 

The present results are a part of investigations carried out on the starting selection material of three populations of the genus Agrostis L. species: A. capillaris L., A. stolonifera L. and A gigantea Roth. The method applied was inbreeding, that is creating of inbred lines of the starting material for cultivars selection. The aim of selection was to develop high-productive forage crops not present in our domestic assortment. The values obtained for plant growth, level of tillering, number of generative shoots. DM yield, seed yield and germination were compared between inbred and mother lines. The selection process was initiated in 1991 when best plants were selected by detailed morphological analyses and phenological observations. Inbreeding was carried out by standard procedure at the optimal stage of generative shoots formation. Plants of S1 lines produced from inbred seeds were tested with mother plants in the following two years. Inbreeding of S1 plants was done then and the obtained S2 lines were tested in the previous two years with mother lines as well, in 50 replications per progeny. The results were processed by the analysis of variance and significance of differences was tested by LSD test. Irrespective of its origin, the tested progeny of 6 populations of the species Agrostis capillaris L. did not show statistically significant difference for plant height. Very significantly higher DM yield was produced by S0 line of the population 1.18 (147.65 g/plant) compared with S2 line (104.58 g/plant). Population S2 1.18 produced very significantly higher DM yield. Seed yield in Si and S2 was very significantly higher compared with S0 in populations 1.27, 1.08 and 1.01, and in population 1.14 seed yield was significantly higher in all progenies. Seed vigor and germination was above 70% in S0 lines 1.26 and 1.27, in Si lines 1.01, 1.14 and 1.27, and in S2 lines from populations 1.14, 1.26 and 1.27. Results for 7 populations of the species Agrostis stolonifera L. show that shoot height was very significantly higher in the population S0 3.02 and significantly higher in the populations S2 3.27 and 3.42. Number of generative shoots and DM yield were very significantly higher in S0 3.08 compared with Si and S2. Seed vigor and total germination was above 70% in S0 3.02, 3.11 and 3.19, in Si 3.24 and 3.27 and in S2 3.02, 3.11, 3.19, 3.24 and 3.42. Traits of S0, Si and S2 lines of 7 populations of the species Agrostis gigantea Roth, were measured. Plant height was very significantly higher in S2 4.08, 4.16 and 4.18 and in St line 4.20. Number of generative shoots was very significantly higher only in the population S0 4.20. DM production was highest in the populations with highest number of shoots. However, seed yield demonstrated statistically very significantly higher difference in Si 4.06, 4.16 and 4.18. Seed germination above 70% was found in populations S0 4.05, 4.08 and 4.18 and in S2 lines in populations 4.02, 4.05 and 4.06. The results obtained for inbreeding depression across two generations of inbreeding as well as comparative testing of lines with mother plants allow for diversity in decision making about further applications of this method and about crossing and creating of hybrid progeny of new cultivars.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

REGENERATION OF PERENNIAL GRASSES SEEDS FROM THE COLLECTION OF THE PLANT GENE BANK

 

Zorica Tomić i Sokolović D.

 

Agricultural Research Institute "Serbia", Center for Forage crops, Krusevac

 

The collection of the plant gene bank of the Center for Forage Crops, Krusevac has been formed of seed samples of perennial grasses populations collected from the flora of Serbia in 1988. Seed samples of 5 perennial grasses species have been kept in the collection of the Yugoslav Plant Gene Bank, Belgrade since 1991. Regeneration of seed samples from the existing collection was done because seed germination had substantially declined during the past years. The collection contains seed samples of 10 perennial ryegrass populations (Lolium perenne L.), 5 cocksfoot populations (Dactylis glomerata L.), 35 colonial bentgrass populations (Agrostis capillaris L.), 33 creeping bentgrass populations (Agrostis stolonifera L.) and 15 red top populations (Agrostis gigantea Roth.). In the fall of 1996, in a glasshouse, each seed sample was separately sown in small rows in a dense stand. Young plantlets were transplanted in the spring of 1997 on a plot 4m2 in size, with 100 plants and replications in a plot. Samples were transplanted on the experimental field in isolation in a wheat crop. The spacing between small plots in isolation was 2m. Seed harvest was done successively, depending on maturing time. Seeds were dried to 5% moisture. The amount of all seed samples was determined and now they are kept in adequate bags till the examinations for germination ability. Afterwards, a 5-g amount of each seed sample will be delivered to the Plant Gene Bank, Belgrade for a long-term keeping under controlled conditions at -20°C. The results for the time of development, i.e. heading pertain to the populations of species, such as Lolium perenne L. May 3-14, Dactylis glomerata L. April 25 -May 5, Agrostis capillaris L. May 28-June 14, Agrostis stolonifera L. June 1-14, and Agrostis gigantea Roth June 6-12. Average seed yield in the samples of populations was:

Lolium perenne L. 162g, Dactylis glomerata L. 387g, Agrostis capillaris L. 121g, Agrostis stolonifera L. 108g, and Agrostis gigantea Roth. 82g. Data on seed vigor and germination for all samples are at and over standard germination level for species, i.e. more than 70 and 85%.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

CLONAL ARCHIVES OF FIR WITH FASTIGIATE CROWN (Abies alba Mill. var. elegantissima Tošić) NEAR IVANJICA - PILOT PLOTS - FOR PROTECTION AND BREEDING

 

Mihailo Tošić

 

d. Tucovića 41/34, Užice

 

Fir variety with a fastigiate crown (Abies alba Mill. var. elegantissima Tosic) at Ogorijevac (south slopes of Mt. Javor) is a spontaneous generative mutant with specific morpho-physiological properties (Tosic, 1963, 1995). An extraordinary botanical rarity in the wider European area, this fir, i.e. its site has been designated a strict nature reserve, which is significant for its protection. However, when natural rarities are living organisms, namely forest trees created by mutation, this basic measure is insufficient for permanent protection. Generative mutants, especially conifers, are often characterized by slow growth compared to normal trees, which can be an advantage in horticulture, but in nature such trees rarely stand the competition of the surrounding faster-growing normal trees. Generative progeny is also variable, so some desirable phenotypes can be absent. In the aim of permanent conservation of this specific genotype, with extraordinary esthetical features, the author and the Forest Estate "Golija" at Ivanjica produced 600 grafts (ramets), of 6 trees (ortets) of generative origin. In the spring 1998 plantations were established at two sites near Ivanjica, i.e. at Raca - 207 and at Klekovica - 150 grafted seedlings. For safety reasons, at both sites, two separate plots were planted, so that a possible fire could not destroy the entire plantation. By heterovegetative reproduction of genotypes discovered at Ogorijevac and by the establishment of clonal plantations: (1) the genotypes are fixed and they will be protected from potential destruction (some trees were felled in the Reserve by unknown persons), (2) seed production from free and controlled fertilization is carried out in the aim of generative reproduction and further breeding and (3) secondary scions are used for further production of grafts, because the branches on younger trees are superior and more accessible than on those already overmature at Ogorijevac.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC POTENTIAL OF YIELD OF NEW ZP MAIZE (Zea mays L.) HYBRID COMBINATIONS

 

Trifunović B.V., Trifunović V., Stanković G.

 

Maize Research Institute, Zemun Polje, 11080 Belgrade-Zemun, Yugoslavia

 

The development of maize hybrids of high genetic potential of yield is based on the idea of the phenomenon of heterosis. Since the period of double-cross, three-way-cross and single cross hybrids, maize breeders have been striving to develop hybrids with top and stable yield, as well as with favourable agronomic traits. According to Trover (1996) the introduction of hybrids into the US agriculture started in 1933, while all areas under maize were planted by the hybrid seed in the beginning of the 1960's. In Yugoslavia, the US hybrids were grown at the end of the 50's. Local hybrids were introduced in the beginning of the 60's, while over 90% of areas under maize were planted with local maize hybrids at the end of the 60's. A 40-year contribution of the Maize Research Institute, Zemun Polje to the increase of the total production of this, the most important field crop for us, amounts to 1% annually (Dumanovic, 1986).

In order to provide stable yield, the aim of the Institute was to develop the new medium maturing hybrids (FAO 400-500) of frequency of favourable alleles controlling yield not lagging behind high yielding hybrids of FAO maturity group 600-700 widely spread in the country (to about 80% areas). The trials with 24 maize hybrids (FAO 300 to FAO 700) were set up in 10 locations and carried out for two years under different agroecological conditions with the application of the common maize cropping practices.

The analysis of obtained results shows that frequency of favourable alleles controlling yield in hybrids of FAO maturity group 600-700 did not significantly change in relation to previously grown maize genotypes. However, the corresponding frequency in the new ZP hybrid combinations within the FAO maturity group 400-500 increased. This is indicated by the average increase of grain yield of these genotypes by 4.72% in relation to hybrids previously spread in the production. The new medium late maturity genotypes with the gene combination resulting in high genetic potential of yield of ZP maize hybrids were developed by directional yield selection and appropriate selection of the initial material (local x exotic germplasm).

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC PARAMETERS OF THE FATTENING AND REPRODUCTIVE TRAITS OF SWEDISH LANDRACE

 

Trivunović Snežana1, Teodorović M.1, Petrović Milica2

 

1 Faculty of Agriculture, Novi Sad

2Faculty of Agriculture, Belgrade-Zemun

 

Reproduction results of 1422 sows of Swedish Landrace breed originating from 10 sires which were performance tested before used in the breeding have been used in the paper. Medium values of heritability 0.48, 0.40, 0.34, 0.55 were found for the lifetime daily gain, fat thickness, age at the end of the test and age at the first farrowing, respectively as well as the low heritability 0.14, 0.04, 0.15 for the reproductive traits such as the number of live-born, still-born and weaned piglets.

Genetic, phenotypic and environmental correlations were in agreement with those found in literature indicating that the selection for the fattening traits has no negative effect on the reproductive ones.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC -ECOLOGICAL BASES OF AILANTHUS (Ailanthus altissima Swingle) ADAPTATION IN SERBIA

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

Faculty of Forestry, Belgrade

 

Based on morpho-physiological analyses, experimental + laboratory tests and literature sources, this paper deals with the bases of ailanthus adaptation, as the allochthonous species in Serbia. The study was performed in several spontaneous populations in which several mother trees were selected and half-sib lines were produced from their seed. The stability and the competitiveness of trees in populations are the result of ontogenetic, population and phylogenetic adaptation. As the process of adaptation is performed at several levels (cell, tree, population, species), each has a specific criterion of adaptation. The results of half-sib line analyses confirm the hypothesis that high adaptation of ailanthus mature trees and populations is in fact a significant protection of the genetic potential of the species. Namely, high heterozygousness of trees stabilizes in the ontogeny the transition of the potential genetic variability into free one, available to natural selection. Although the time period between the program of ontogeny and population variability is very short, positive effects have already been recorded at individual and population levels (plus trees, minus trees, etc.). However, their long-time (phylogenetic) aspects can be programmed only theoretically, e.g. through the so-called complex model of tree and shrub breeding in Serbia. Phylogenetic adaptation, through successive generations, has a decisive role in ailanthus adaptation, not only within the scope of the cultigeneous but also within its total range of distribution.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

FLOWERING OF Acer saccharinum L. OCCURRENCE, FREQUENCY OF POLYGAMY AND SEX MOSAICS

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

 

Šumarski fakultet, Beograd

 

An important place in tree breeding belongs to sexuality and flowering phenophase. Sexuality and flowering phenophase (inflorescences, flowers and trees) of fifty trees grown at the site of Quercetumfrainetto-cerris s.l. were analyzed. There are five types of inflorescences, flowers and six types of trees. Polygamy was recorded on 14% trees and sexual mosaics on 2%. Phenotypic and genetic differences between the parts of mosaic trees result from somatic mutations, which have an important role in the formation of sex-mosaics. The causes of sex mosaics and polygamous trees are in the scope of hypotheses, while their evident suppression within a tree is based on reparation mechanisms. Sex differences of raised trees lead to differences in pollination, i.e. to higher variability of fruits - heterocarpy, i.e. to changes in genetic structures of successive generations. The obvious practical aspect of the determined variability, starting from the nature and distribution of sexuality and reproduction specificity (allogamy, autogamy, apomixis) is that it enables the spontaneous and planned transformation of the species in our country.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

NEOTENY TYPES IN TREES AND SHRUBS AND THEIR INPORTACE

 

Aleksandar Tucović, Vasilije Isajev i Mirjana Šijačić-Nikolić

 

Faculty of Forestry, Belgrade

 

Early flowering of ailanthus and chaste tree is a cardinal process, which enables their specific adaptation. Premature sexual maturity (neoteny) i.e. the development of reproductive organs in the juvenile stage, is significant as it precedes fructification. This paper describes neoteny in ailanthus seedlings and in 2-3-month old root suckers of chase trees. The number of flowers and the structure of inflorescences in neoteny are significantly simplified. The flowers of ailanthus seedlings are individual and terminal, and the inflorescences in chase trees are much shorter, narrower, unbranched and vertical. Neoteny can be spontaneous - retentive and induced - stimulative. Neoteny is explained by the changes of interactions of trophic, hormonal and genetic systems. A narrower explanation of interaction mechanisms is one of the most complex fields of tree and shrub physiology. The properties of juvenile organisms with very early flowering indicate that the genetic and physiological base of neoteny is very old and that it can be classified as an example of series of parallel hereditary variability, occurring in phylogenetically very distant families, genera and species.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

BALKAN MAPLE GENERATIVE SEED ORCHARD I NEAR IVANJICA - PILOT PLOT FOR BALKAN MAPLE BREEDING

 

Gordana Ćurčić1, Vasilije Isajev2, Mihailo Tošić3

 

1 JP "Srbijasume'Torest Estate "Golija" Ivanjica

2Faculty of Forestry, Belgrade,

3D. Tucovića 41/34, Užice

 

The enhancement and intensifying of technically valuable timber production requires the increased share of valuable broadleaves in our forests. In this aim, valuable broadleaf seedlings (sycamore, ash, cherries, etc.) are increasingly produced in forest nurseries. Seed orchards are preferable in order to achieve the desirable properties of seeds and nursery stock for afforestation programs. Balkan maple (Acer heldreichii Orph.), the Balkan Peninsula relic and endemic, is distributed in the higher parts of mountains in Serbia and wider. On the mountains Golija and Javor it has optimal conditions for development, so its trees attain imposing dimensions (diameter often above 50 cm and height up to 25 m). The representation of superior trees in the autochthonous population of Balkan maple, its good-quality technical wood and its exceptional pioneer role in natural regeneration of the destroyed (fire or other agents) forest vegetation, were decisive factors for the establishment of Balkan maple seed stands and superior seed trees in the territory of Forest Estate "Golija" at Ivanjica. Aiming at producing selected seed and further breeding of this species, a generative seed orchard - pilot plot was established in 1994 on 105.54 ha near Ivanjica on the site of as Fagetum montanum Rud. By planting 2962 seedlings in 26 half-sib families from seed trees selected as superior for their morphological (technical) and physiological properties, we created the basis for the production of good quality seed for reproduction and further breeding of this valuable species. This paper presents the method and technology of plantation establishment, survival and seedling development during three growth seasons after planting.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

ESTABLISHING RESISTANCE TO Verticillium albo atrum IN THE PROGENY OF ALFALFA (Medicago sativa L.) GENOTYPES

 

Biljana Urošević, Jasmina Radović, Zorica Tomić

 

Agricultural Research Institute "Serbia", Center for Forage Crops, Krusevac

 

Verticillium albo atrum causes root and crown rot in alfalfa, which is followed by wilting. By developing persistent mycelium and swollen walls, the parasite thrives well in the soil regardless of its acidity. Alfalfa vascular diseases, such as rotting, wilting, dying from exposure to freezing are all typical symptoms of this pathogen presence. The nature of the disease makes chemical control ineffective and therefore the only solution is selection for resistance. To determine resistance to Verticillium albo atrum, we have tested the progeny of 14 genotypes using the root cut and dip method. Comparisons were made with internationally recognized testers for this feature, such as variety Maris Kabul - a positive tester and varieties Evropa and Everest - negative testers. Eight weeks after planting of the selected material, sprouted plantlets were taken out from the substrate, root and above-ground parts were cut, and plantlets were dipped in this pathogen suspension. After dipping of the root system, the remaining part of inoculum was put into the substrate, where plants were planted. Six weeks after inoculation, observations of the survived material were carried out. Foliar symptoms were assessed on a 0-5 scale. Resistance of the studied material is presented in % of resistant plants and by DSI index. The results show that our domestic material is substantially resistant to the examined pathogen compared with a positive tester, variety Maris Kabul that had 68.83% of survived plants and DSI index 2.48. When compared with a negative tester, variety Everest (13.75% of survived plants and DSI index 4.40), progeny of 7 genotypes exhibited statistically significant resistance (26.GO-61.73% of resistant plants). When compared with another negative tester, variety Evropa (28% of survived plants and DSI index 3.74), progeny of 6 genotypes manifested higher resistance, while progeny of only one genotype had statistically significantly better resistance. On the basis of % of resistant plants and DSI index, it can be concluded that 4 examined genotypes (2.26-3.35 DSI index) manifested satisfactory resistance to this pathogen, though considerably lower than a positive tester, and they will be taken for further selection. Attention should be also paid particularly to individual plants of susceptible genotypes, for they may also be the carriers of resistance to this pathogen, considering high alfalfa variability. Further studies will focus on establishing resistance inheritance and on production of alfalfa lines resistant to Verticillium albo atrum.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

APPLICATION OF PROTEIN MARKERS IN "GENETIC PURITY" TESTING OF MAIZE GENOME

 

Filipović M., Drinić Mladenović S., Milojević G. i Konstantinov K.

 

Maize Research Institute, 11080 Zemun Polje-Belgrade

 

Maintenance of genetic uniformity of maize inbred lines and hybrids is one of request for successful commercial hybrid seed production. Embryo salt soluble proteins as genetic markers for parental lines and commercial hybrids genotypic identification as well as "genetic purity" determination were used. All study hybrids, as well as, their parental components have specific protein pattern. Some of the protein fractions appeared for each genotype at the same position and could be used as reference bands. Protein fraction common for both parents or inherited only from one parent as well as hybrid specific protein, fraction were detected in the hybrids.; The uniform protein pattern was found in all individual samples in all studied hybrids, pointing to genetic purity of observed seed.

 

Return to content



 

Published in Book of Abstracts „SECOND CONGRESS OF SERBIAN GENETICISTS“

Sokobanja, Serbia, November 10-13, 1999.

© 1999 Serbian Genetics Society

 

 

GENETIC DIVERSITY OF STEM TRAITS IN WINTER WHEAT