GENETIKA, Vol. 32, No.2 (2000)
Snežana DRINIĆ MLADENOVIĆ, Kosana KONSTANTINOV, Goran DRINIĆ, and Dražen JELOVAC
APPLICATION OF MOLECULAR MARKERS AND BIOINFORMATICS IN PLANT BREEDING [Abstract]
Ljubiša TOPISIROVIĆ, Milan KOJIĆ, Đorđe FIRA, Nataša MILADINOV, Ivana STRAHINIĆ, Olivera GAJIĆ, Slavica ARSENIJEVIĆ, Irena SPASOJEVIĆ, and Maja VUKASINOVIĆ
MOLECULAR GENETICS OF AUTOCHTHONOUS LACTIC ACID BACTERIA [Abstract]
Ljiljana RAKIĆEVIĆ, Dubravka GEORGIJEVIĆ, Gordana JANKOVIĆ, Danijela MIKOVIĆ, and Dragica RADOJKOVIĆ
GENETIC PREDICTION OF HEMOPHILIA A BY INT18/BClI RFLP ANALYSIS [Abstract]
Milorad ROŠULJ, Ivan HUSIĆ, and Slobodan TRIFUNOVIĆ
GENETIC ADVANCE IN MAIZE (ZEA MAYS L.) TEST TRIALS IN RELATION TO NUMBER OF LOCATIONS, REPLICATIONS AND HYBRIDS [Abstract]
Olivera SPASIĆ-BOŠKOVIĆ, Tanja LAZIĆ, Grozdana BILIĆ, and Mladen VUJOŠEVIĆ
C-BANDING KARYOTYPES AND LOCATION OF NOR’S IN THE TOAD SPECIES BUFO BUFO AND BUFO VIRIDIS FROM YUGOSLAVIA [Abstract]
Anna NIKOLIĆ, Olgica NEDIĆ, and Romana MASNIKOSA
PERIPHERAL SERUM CONCENTRATIONS OF INSULIN-LIKE GROWTH FACTORS (IGF-I AND IGF-II) DECREASE WITH AGE IN HEALTHY ADULTS [Abstract]
Nada HLADNI, Dragan ŠKORIĆ, and Marija KRALJEVIĆ-BALALIĆ
VARIANCE COMPONENTS AND GENE EFFECTS OF MORPHOLOGICAL TRAITS IN SUNFLOWER (HELIANTHUS ANNUUS L.) [Abstract]
Dragoljub MAKSIMOVIĆ, Dušan UROŠEVIĆ, and Aleksandar PAUNOVIĆ
INHERITANCE OF RESISTANCE TO LOW TEMPERATURES IN THE F1 GENERATION IN DIFFERENT BOTANICAL VARIETAL TYPES OF BARLEY (HORDEUM SATIVUM JESSEN.) [Abstract]
Aleksandra NASTASIĆ, Milisav STOJAKOVIĆ, Đorde JOCKOVIĆ, Goran BEKAVAC, Nenad VASIĆ, and Zoran PETROVIĆ
EFFECT OF S1 RECURRENT SELECTION ON THE OCCURRENCE OF STALK, EAR AND ROOT ROT (FUSARIUM GRAMINEARUM) IN THE SYNTHETIC CORN POPULATION NSB [Abstract]
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 93-113
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 577.218; 575.827
Review article
APPLICATION OF MOLECULAR MARKERS AND BIOINFORMATICS IN PLANT BREEDING
Snežana DRINIĆ MLADENOVIĆ, Kosana KONSTANTINOV, Goran DRINIĆ, and Dražen JELOVAC
Maize Research Institute “Zemun Polje”, 11080 Zemun-Belgrade, Yugoslavia
Abstract
Drinić Mladenović S., K. Konstantinov, G. Drinić, and D. Jelovac (2000): Application of molecular markers and bioinformatics in plant breeding. – Genetika, Vol. 32, No. 2, 93-113.
In the past decade development of molecular genetics brought new dimension of a plant breeding. Molecular markers as universaly methods for all biological system virtualy effect on a success of directly examination of structure and function of genome and well as determination of genetic polymorphism of plant genomes. The potential applications of molecular markers in plant breeding are: analysis of molecular basis of evolution; germ plasm identification, classification and managment; assessing genetic diversity; identification of genes underlying agronomy important traits as yield, resistence to stress and dissease as well as heterosis. High density genetic linkage maps for a number of plant species as a basis for marker assisted selection of agronomically useful traits and isolation of these genes have been established. Important factor in the successes of the genetic improvement of crops was the development of faster and more reliable methods, which allowed easier analysis- of date as well as rapid information excange. The application of information tehnology and development of statistical techniques to analyze genomic information is know as “bioinformatics”. By integrating genetics with informatics investigations of whole genomes aims to elucidate the structure, function and evolution of plant genomes are faster. Together these technologies as integral part of classical breeding programs contibuting significantlly to shorting of plant breeding process and cycles of selection.
Key words: molecular markers, bioinformatics
Corresponding author: Snežana Drinić Mladenović, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11080 Zemun, Yugoslavia
e-mail: msnezana@mrizp.co.yu
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 115-127
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 579.6
Review article
MOLECULAR GENETICS OF AUTOCHTHONOUS LACTIC ACID BACTERIA
Ljubiša TOPISIROVIĆ, Milan KOJIĆ, Đorđe FIRA, Nataša MILADINOV, Ivana STRAHINIĆ, Olivera GAJIĆ, Slavica ARSENIJEVIĆ, Irena SPASOJEVIĆ, and Maja VUKASINOVIĆ
Institute of Molecular Genetics and Genetic Engineering,
11001 Belgrade, Yugoslavia
Abstract
Topisirović Lj., M. Kojić, Đ. Fira, N. Miladinov, I. Strahinić, O. Gajić, S. Arsenijević, I. Spasojević, and M. Vukasinović (2000): Molecular genetics of autochthonous lactic acid bacteria. – Genetika, Vol. 32, No. 2, 115-127.
Autochthonous strains of lactic acid bacteria (LAB) were isolated from homemade fermented milk products. These products were collected from specific ecological localities such as high mountains (above 1200 m above sea level), mountain plateaus, river valleys, islands, Adriatic coast, etc. Analysis of LAB from the collection of natural isolates revealed that they produce proteinases, bacteriocins and exopolysaccharides. It was also shown that some isolates of lactococci and lactobacilli produce two bacteriocins simultaneously. According to their antimicrobial and biochemical properties, most of the analysed bacteriocins in natural isolates of lactococci were class II bacteriocins. In addition some isolates produced both proteinase and bacteriocin. Biosynthesis of specific proteinases was detected in natural isolates. Analysis of the prt gene organisation among natural isolates of LAB showed that four isolates of lactobacilli exibited prt genes organisation different from those described so far. Elucidation of the regulation of the genes encoding proteinases, bacteriocins and exopolysaccharides could facilitate the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. fermented products with a geographical origin.
Key words: Autochthonous LAB, bacteriocins, exopolysaccharides, proteinases, plasmid
Corresponding author: Dr Ljubiša Topisirović, Laboratory for Molecular Genetics of Industrial Microorganisms, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P. O. Box 446, 11001 Belgrade, Yugoslavia
Tel: +381 11 397 59 60
Fax: +381 11 397 58 08
E-mail: LAB6@EUnet.yu
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 129-135
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.113.2; 616.151.5
Original scientific paper
GENETIC PREDICTION OF HEMOPHILIA A BY INT18/BClI RFLP ANALYSIS
Ljiljana RAKIĆEVIĆ1, Dubravka GEORGIJEVIĆ1, Gordana JANKOVIĆ2, Danijela MIKOVIĆ2, and Dragica RADOJKOVIĆ1
1Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia
2National Blood Transfusion Institute, Belgrade, Yugoslavia
Abstract
Rakićević Lj., D. Georgijević, G. Janković, D. Miković, and D. Radojković (2000): Genetic prediction of hemophilia a by INT18/BCLI RFLP analysis. – Genetika, Vol. 32, No. 2, 129-135.
Hemophilia A, an X linked genetic disease, is the most common coagulation disorder with an incidence of about 1-2 in 10 000 males. It is caused by mutations in the factor VIII coagulation gene. After cloning of the factor VIII gene, almost all types of mutations causing hemophilia A were characterized. The wide spectrum of different mutations in the factor VIII gene made direct DNA diagnosis of the disease not the method of choice. Indirect DNA diagnosis, using restriction fragment length polymorphisms (RFLPs) offers an alternative. The aim of our study was to provide carrier and prenatal diagnostics for affected families using the indirect approach. The genomic DNA of 67 members of 24 hemophilia A families were analyzed by polymerase chain reaction (PCR) amplification of the BclI polymorphic region at intron 18 of the factor VIII gene. Twelve families were informative for int18/BclI RFLP (50%). One prenatal diagnosis of hemophilia A was performed in the first trimester of gravidity, and the fetus was found to be a male affected by hemophilia A. Indirect DNA testing is straightforward, rapid and inexpensive to perform. Thus, in many families requiring genetic diagnosis of hemophilia A, as well as genetic counselling, the use of intragenic polymorphism analysis represents the diagnostic strategy of choice.
Key words: hemophilia A, prenatal diagnosis, carrier detection, RFLP
Corresponding author: Ljiljana Rakićević, Institute of Molecular Genetics and Genetic Engineering, 11001 Belgrade, Yugoslavia, Vojvode Stepe 444a, P.O. Box: 446
tel. +381-11-491391, fax: +381-11-3975808
e-mail: QWERT@Eunet.YU
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 137-143
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
GENETIC ADVANCE IN MAIZE (ZEA MAYS L.) TEST TRIALS IN RELATION TO NUMBER OF LOCATIONS, REPLICATIONS AND HYBRIDS
Milorad ROŠULJ, Ivan HUSIĆ, and Slobodan TRIFUNOVIĆ
Maize Research Institute “Zemun Polje”, 11080 Belgrade-Zemun, Yugoslavia
Abstract
Rošulj M., I. Husić, and S. Trifunović (2000): Genetic advance in maize (Zea mays L.) test trials in relation to number of locations, replications and hybrids. – Genetika, Vol. 32, No. 2, 137-143.
With a view to reducing expenses and errors at planting, harvesting and during further work on the material, it is more practical to set up trials with a lower replication number per investigated location. Thereby, the location number might be increased. Such trials provide better sampling of environmental conditions. This study encompassed 280 yield test trials conducted at the Maize Research Institute (MRI) from 1994 to 1999. Trials were set up according to the RCB design with 1 to 4 replications and 2 to 11 locations. Average genetic advance was estimated by the mathematical model of Spraque and Federer (1951). Based on the results obtained it can be concluded that a higher genetic advance can be provided with an increase in number of locations and hybrids and a decrease in the number of replications within locations.
Key words: maize, genetic advance, number of replications, number of locations, and number of hybrids
Corresponding author: Milorad Rošulj, Maize Research Institute, Zemun Polje, 11080 Belgrade-Zemun, Yugoslavia
e-mail: mrosulj@yahoo.com
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 145-153
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.853; 597.82
Original scientific paper
C-BANDING KARYOTYPES AND LOCATION OF NOR’S IN THE TOAD SPECIES BUFO BUFO AND BUFO VIRIDIS FROM YUGOSLAVIA
Olivera SPASIĆ-BOŠKOVIĆ, Tanja LAZIĆ, Grozdana BILIĆ,
and Mladen VUJOŠEVIĆ
Department of Genetics, Institute for Biological Research “Siniša Stanković”, 11060 Belgrade, Yugoslavia
Abstract
Spasić-Bošković O., T. Lazić, G. Bilić, and M. Vujošević (2000): C-banding karyotypes and location of NOR’s in the toad species Bufo bufo and Bufo viridis from Yugoslavia. – Genetika, Vol. 32, No.2, 145-153.
The karyotypes, C-banding patterns and positions of nucleolus organizer regions (NORs) of two European species of toads, Bufo bufo and Bufo viridis from seven localities in Yugosiavia were investigated. These karyotypes and chromosomai markers were compared with those in other species and subspecies of the Bufo bufo complex. The results suggest that C-banded patterns are characters specific for those species. A marker band on chromosome 5 in the karyotype of B, bufo bufo is shown.
Key words: Bufo bufo, Bufo viridis, constitutive heterochromatin, NORs
Corresponding author: Mladen Vujošević, Department of Gentics, Institute for Biological Research “Siniša Stanković”, 29. Novembra 142, 11060 Belgrade, Yugoslavia
e-mail: mladenvu@ibiss.bg.ac.yu
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 155-165
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22
Original scientific paper
PERIPHERAL SERUM CONCENTRATIONS OF INSULIN-LIKE GROWTH FACTORS (IGF-I AND IGF-II) DECREASE WITH AGE IN HEALTHY ADULTS
Anna NIKOLIĆ, Olgica NEDIĆ, and Romana MASNIKOSA
Institute for the Application of Nuclear Energy, 11080 Zemun-Belgrade, Yugoslavia
Abstract
Anna Nikolić, O. Nedić and R. Masnikosa (2000): Peripheral serum concentrations of insulin-like growth factors (IGF-I and IGF-II) decrease with age in healthy adults. – Genetika, Vol. 32, No. 2, 155-165.
The aim of this investigation was to determine the concentrations of the insulin like growth factors (IGF-I and IGF-II) in the blood serum of normal healthy adults of different ages. Peripheral blood samples were obtained from a total of 52 men and 45 women aged 18-75 years. After separation of the serum, IGF-I and IGF-II concentrations were determined in neutralised acid-ethanol extracts by validated liquid phase radioimmunoassays using appropriate highly specific anti-IGF antibodies as the reagents in each test. The overall mean concentrations of these growth factors were 26.2 (SD 9.7) and 73.4 (SD 14.6) nmol/l respectively for IGF-I and IGF-II and the values found were positively correlated (r = 0.460, P < 0.001). Serum levels of immunoreactive IGF-I declined with increasing age at similar slopes in both men (r = -0.525, P < 0.001) and women (r = -0.422, P = 0.004). There was an equivalent fall in immunoreactive IGF-II concentrations with age in women (r = -0.514, P < 0.001) but the age-related decrease in men was small and not statistically significant (r = -0.251, P = 0.072). These physiological data obtained from the local population will form the basis for comparison with serum IGF levels found in patients suffering from different diseases, such as acromegaly, growth hormone deficiency and liver cirrhosis. The importance of taking age into consideration when assigning normal concentration ranges is emphasised.
Key words: human serum, IGF-I, IGF-II, radioimmunoassay, age effects
Corresponding author: Dr Anna Nikolić, INEP - Institute for the Application of Nuclear Energy, Banatska 31B, 11080 Zemun. Yugoslavia
tel: 381-11-617252
fax: 381-11-618724
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 167-174
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
VARIANCE
COMPONENTS AND GENE EFFECTS OF MORPHOLOGICAL TRAITS IN SUNFLOWER
(HELIANTHUS ANNUUS L.)
Nada HLADNI1, Dragan ŠKORIĆ1, and Marija KRALJEVIĆ-BALALIĆ2
1Institute of Field and Vegetable Crops, 21000 Novi Sad,
2Institute of Field and Vegetable Crops, Faculty of Agriculture, 21000 Novi Sad, Yugoslavia
Abstract
Hladni N., D. Škorić, and M. Kraljević-Balalić (2000): Variance components and gene effects of morphological traits in sunflower (Helianthus annuus L.). Genetika, Vol. 32, No. 2, 167-152.
In order to enable progress in sunflower breeding, an increasing amount of attention has to be paid to the morpho–physiological traits (petiole angle and length) of this crop that have a significant effect on its seed yield and oil concentration. In the inheritance of petiole angle and petiole length the additive component of genetic variance had the predominant role in the F1 and F2 generation. The mean degree of dominance (H1/D)1/2 and the intersection of the expected regression line with the Wr axis indicated partial dominance in the inheritance of petiole angle and petiole length. Broad sense heritability for petiole angle was 88% and for petiole length 85%.
Key words: sunflower, morpho-physiological, inheritance, gene effect, heritability
Corresponding author: Nada Hladni, Institute for Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Yugoslavia
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 175-180
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.12; 580.036
Original scientific paper
INHERITANCE OF RESISTANCE TO LOW TEMPERATURES IN THE F1 GENERATION IN DIFFERENT BOTANICAL VARIETAL TYPES OF BARLEY (HORDEUM SATIVUM JESSEN.)
Dragoljub MAKSIMOVIĆ1, Dušan UROŠEVIĆ1, and Aleksandar PAUNOVIĆ2
1Centre for Small Grains, 34 000 Kragujevac
2Faculty of Agronomy, 32 000 Čačak, Yugoslavia
Abstract
D. Maksimović, D. Urošević, A. Paunović (2000): Inheritance of resistance to low temperatures in the F1 generation in different botanical varietal types of barley (Hordeum sativum Jessen.). – Genetika, Vol. 32, No. 2, 175-180.
The results for inheritance of resistance to low temperatures in F1 generation hybrids of different botanical varieties of barley are presented in this paper. Correlation between resistance to low temperatures and some morpho-physiological characteristics of parents of different botanical variety and their F1 generation hybrids was found. The three cross combinations between different barley cultivars were carried out using the scheme: unresistant x unresistant, unresistant x resistant, resistant x resistant.
Key words: barley, botanical variety, inheritance, F1 generation, correlation.
Corresponding author: Dragoljub Maksimović, Centre for Small Grains, 34000 Kragujevac, Yugoslavia
Published in „GENETIKA“ Vol. 32, No.2 (2000), pp. 181-188
© 2000 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.827
Original scientific paper
EFFECT OF S1 RECURRENT SELECTION ON THE OCCURRENCE OF STALK, EAR AND ROOT ROT (FUSARIUM GRAMINEARUM) IN THE SYNTHETIC CORN POPULATION NSB
Aleksandra NASTASIĆ, Milisav STOJAKOVIĆ, Đorde JOCKOVIĆ, Goran BEKAVAC, Nenad VASIĆ, and Zoran PETROVIĆ
Institute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia
Abstract
Nastasić A., M. Stojaković, Đ. Jocković, G. Bekavac, N. Vasić, and Z. Petrović (2000): Effect of S1 recurrent selection on the occurrence of stalk, ear and root rot (Fusarium graminearum) in the synthetic corn population NSB. – Genetika, Vol. 32, No. 2, 181-188.
A study has been conducted to assess the effect of S1 recurrent selection on the occurrence of stalk, ear and root rot (Fusarium graminearum) in the synthetic corn population NSB. After five cycles of S1 recurrent selection, a significant increase was registered in the stalk resistance to the agent of stalk rot. The percentage of lodged plants was reduced from 57.7 to 41.6%. The largest reduction in the number of lodged plants occurred in cycle C5. In all selection cycles, lower percentages of plants infected by stalk rot occurred in the locations of Ada and Sombor, in conditions of natural infection, than in the locations Rimski Šančevi and Srbobran, in conditions of inoculation.
Key words: corn, S1 recurrent selection, infection index
Corresponding author: Aleksadra Nastasić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Yugoslavia