GENETIKA, Vol. 42, No. 2 (2010)
Pranveer SINGH and B.N. SINGH
POPULATION GENETICS OF Drosophila ananassae: CHROMOSOMAL ASSOCIATION STUDIES IN INDIAN POPULATIONS [Abstract] [Full text]
Jasmina ZdravkoviC, Nenad PavloviC, Zdenka Girek, Milan ZdravkoviC and Dejan CvikiC
characteristics IMPORTANT for organic breeding of vegetable crops
Ivana PEJIN-GRUBIŠA, Ivana BUZADŽIC, Biljana JANKOVIĆ-OREŠČANIN, Nada BARJAKTAROVIĆ-VUČINIĆ
DISTRIBUTION OF PARAOXONASE 1 CODING REGION POLYMORPHISMS IN SERBIAN POPULATION [Abstract] [Full text]
Zorica Nikolić, Sanja Vasiljević, Đura Karagić, Milka Vujaković, Dušica Jovičić, Slobodan Katić, Gordana Šurlan-Momirović
Genetic diversity of red clover cultivars (Trifolium pratense L.) based on protein polimorphism [Abstract] [Full text]
Janko ČERVENSKI, Jelica GVOZDANOVIĆ-VARGA, Mirjana VASIĆ, Svetlana GLOGOVAC
MULTIVARIATE ANALYSIS FOR HEAD WEIGHT AND YIELD PERFORMANCE OF EXPERIMENTAL CABBAGE HYBRIDS (Brassica oleracea var. capitata L.)
Dragana IGNJATOVIĆ-MICIĆ, Goran STANKOVIĆ, Ksenija MARKOVIĆ, Snežana MLADENOVIĆ DRINIĆ, Vesna LAZIĆ-JANČIĆ, and Miloje DENIĆ
kernel modifications and tryptophan content in QPM segregating generations [Abstract] [Full text]
Biljana GORJANOVIĆ, Miroslav ZORIĆ, Marija KRALJEVIĆ-BALALIĆ
EFFECT OF NITROGEN RATE ON GRAIN YIELD OF BREAD WHEAT GENOTYPES [Abstract] [Full text]
Slobodan DRAZIC, Slaven PRODANOVIC and Tomislav ZIVANOVIC
IDENTIFICATION OF DONOR LINES CONTAINING FAVORABLE ALLELES FOR QUANTITATIVE TRAITS IN BURLEY TOBACCO (NICOTIANA TABACUM L.)
Sanja RADIČEVIĆ, Radosav CEROVIĆ, Ivana GLIŠIĆ, and Žaklina KARAKLAJIĆ-STAJIĆ
PROMISING SOUR CHERRY HYBRIDS (Prunus cerasus L.) DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK [Abstract] [Full text]
Željana PRIJIĆ and Zoran JERKOVIĆ
GENETIC BASE OF DURABLE RESISTANCE TO Puccinia triticina OF TWO SERBIAN VARIETIES [Abstract] [Full text]
Vojka Babić, Zorica Pajić, Slaven Prodanović, Milosav Babić and Milomir Filipović
VISUAL ASSESSMENT OF SWEET MAIZE LINES PHENOTYPE, ACCORDING TO UPOV DESCRIPTOR, AS INDICATOR OF HETEROSIS [Abstract] [Full text]
Miroslav Malešević, Đorđe GLAMOČLIJA, Novo Pržulj, Vera POPOVIĆ, Saša STANKOVIĆ , Tomislav ŽIVANOVIĆ and Angelina TAPANAROVA
PRODUCTION CHARACTERISTICS OF DIFFERENT MALTING BARLEY GENOTYPES IN INTENSIVE NITROGEN FERTILIZATION [Abstract] [Full text]
Ahmad DADASHPOUR, Alireza TALAEI and Ali SHAHI-GHARAHLAR
EFFECT OF ‘GUTINGEN V’ AS AN INTENSIVE TRAINING SYSTEM ON AGROMORPHOLOGICAL CHARACTERS OF SOME APPLE CULTIVARS IN KARAJ REGION OF IRAN [Abstract] [Full text]
INBRED LINES OF DIFFERENT CYCLES OF SELECTION AS DONORS OF favourable alleles FOR THE IMPROVEMENT OF F1 MAIZE HYBRIDS
Jela IKANOVIC, Đorđje GLAMOCLIJA, Radojka MALETIC,Snezana JANKOVIC, Marjenka TABAKOVIĆ and Ljubisa Zivanovic
THE GENOTYPE TRAITS OF FORAGE SORGHUM, SUDAN GRASS AND THEIR INTERSPECIES HYBRID IN THE CONDITIONS OF INTENSIVE NITROGEN NUTRITION
Slađana MARIĆ, Milan LUKIĆ, Radosav CEROVIĆ, Milisav MITROVIĆ and Radovan BOŠKOVIĆ
APPLICATION OF MOLECULAR MARKERS IN APPLE BREEDING
Aleksandra NIKOLIC, Aleksandra DIVAC, Marija STANKOVIC, Jelena DINIC, Snezana LUKIC, Marina ANDJELIC-JELIC, Dragan POPOVIC, Dragica RADOJKOVIC
PREVALENCE OF S AND Z ALPHA 1-ANTITRYPSIN MUTATIONS IN PATIENTS WITH PANCREATIC DISEASES IN SERBIAN POPULATION [Abstract] [Full text]
Dragan NIKOLIĆ and Dragan MILATOVIĆ
EXAMINING SELF-COMPATIBILITY IN PLUM (Prunus domestica L.)
BY FLUORESCENCE MICROSCOPY [Abstract] [Full text]
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 210-222
© 2010 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 596.77
Original scientific paper
POPULATION GENETICS OF Drosophila ananassae: CHROMOSOMAL ASSOCIATION STUDIES IN INDIAN POPULATIONS
Pranveer SINGH and B.N. SINGH
Genetics laboratory, Department of Zoology, Banaras Hindu University,
Varanasi – 221 005, India
Abstract
Singh P. and B.N Singh (2010): Population genetics of Drosophila ananassae: chromosomal association studies in Indian populations. - Genetika, Vol 42, No. 2, 210 - 222.
Forty-five natural populations of Drosophila ananassae and laboratory stocks made from these flies were analysed for chromosome inversions. Quantitative data on the frequencies of these inversions were utilized to test intra- and interchromosomal interactions in D.ananassae. In most of the natural as well as laboratory populations no significant deviation from randomness of intra- and interchromosomal associations (2L-3L, 2L-3R, 3L-3R) was found hence, providing evidence for random associations. However, in some instances, significant deviation from randomness was found in both natural and laboratory populations, which could be due to excess of certain combinations, deficiency of others and complete absence of some combinations. Possible role of genetic drift could be implicated due to tight-linkage between linked gene arrangements. This strengthens the previous suggestion that there is lack of genetic coadaptation in D. ananassae
Key words: Drosophila ananassae, inversions, intra- and interchromosomal associations, epistatic selection, random genetic drift
Corresponding author: B.N. Singh, Department of Zoology, Banaras Hindu University,Varanasi – 221 005, India,bnsingh@bhu.ac.in; bashisthsingh2004@rediffmail.com
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 223 -233
© 2010 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.635.1/.8
Original scientific paper
characteristics IMPORTANT for organic breeding of vegetable crops
Jasmina ZdravkoviC1, Nenad PavloviC1, Zdenka Girek1,
Milan ZdravkoviC1 and Dejan CvikiC1
1 Institute for Vegetable Crops, Smederevska Palanka, Serbia
Abstract
Zdravković J., N. Pavlović, Z. Girek, M.Zdravković and D. Cvikić (2010): Characteristics important for organic breeding of vegetable crops- Genetika, Vol 42, No. 2, 223 -233.
The remarkable development and application of new genetic The Institute for Vegetable Crops possesses a rich germplasm collection of vegetables, utilized as gene resource for breeding specific traits. Onion and garlic breeding programs are based on chemical composition improvement. There are programs for identification and use of genotypes characterized by high tolerance to economically important diseases. Special attention is paid to breeding cucumber and tomato lines tolerant to late blight. As a result, late blight tolerant pickling cucumber line, as well as late blight tolerant tomato lines and hybrids are realized. Research on bean drought stress tolerance is initiated. Lettuce breeding program including research on spontaneous flora is started and interspecies hybrids were observed as possible genetic variability source. It is important to have access to a broad range of vegetable genotypes in order to meet the needs of organic agriculture production. Appreciating the concept of sustainable agriculture, it is important to introduce organic agriculture programs in breeding institutions.
Key words: disease tolerance, organic breeding, specific traits, vegetable germplasm
Corresponding author: Jasmina Zdravkovic, Institute for Vegetable Crops, Karađorđeva 71, 11420 Smederevska Palanka, Serbia, Phone: 381 26 323 170, fax 381 26 323 785, e-mail: jzdravkovic@institut-palanka.co.rs
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 235 - 247
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
DISTRIBUTION OF PARAOXONASE 1 CODING REGION POLYMORPHISMS IN SERBIAN POPULATION
Ivana PEJIN-GRUBIŠA1*, Ivana BUZADŽIC 1, Biljana JANKOVIĆ-OREŠČANIN2, Nada BARJAKTAROVIĆ-VUČINIĆ1
1 Department of human genetics and prenatal diagnostics, University Medical Hospital Zvezdara, Belgrade, Serbia
2 Blood Transfusion Center, University Medical Hospital Zvezdara, Belgrade, Serbia
Abstract
Pejin Grubiša I., I. Buzadžić, B. Janković-Oreščanin, and N. Barjaktarović Vučinić (2010): Distribution of paraoxonase 1 coding region polymorphisms in Serbian population- Genetika, Vol. 42, No. 2, 235 - 247.
Serum paraoxonase 1 (PON1) in humans is a protein component of high-density lipoprotein (HDL) particles that protects against oxidative damage, detoxifies toxic metabolites of organophosphorus pesticides and nerve agents and activates or inactivates specific drugs. It has been reported that PON1 gene coding region polymorphisms, L55M and Q192R, could influence both expression level and catalytic activity of PON1, and their link with a broad spectrum of diseases has been described. The aim of this study was to determine the frequencies of PON1 coding region polymorphisms Q192R and L55M in Serbian population. The most frequent alleles were Q (0.77) for Q192R and L (0.68) for L55M. Genotypes QQ (0.60) and LL (0.47) and combined genotype QQ/LL (0.26) were the most frequent in examined population.
Key words: coding region polymorphisms, paraoxonase I, Serbian population
Corresponding author: Pejin-Grubisa Ivana, University Medical Hospital Zvezdara, Department of human genetics and prenatal diagnostics, Dimitrija Tucovica 161, 11 000 Belgrade, Serbia. Tel: + 381 11 3810 600, Mail: i.pejin@lab.kbczvezdara.co.rs, ivanapejin@yahoo.com
Published in „GENETIKA“ Vol. 42, No.2 (2010), pp. 249 -258
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.33
Original scientific paper
Genetic diversity of red clover cultivars (Trifolium pratense L.) based on protein polimorphism
Zorica Nikolić 1, Sanja Vasiljević 1, Đura Karagić 1, Milka Vujaković 1, Dušica Jovičić1, Slobodan Katić1, Gordana Šurlan-Momirović 2
1Institute of Field and Vegetable Crops, Novi Sad
2University of Belgrade, Faculty of Agriculture, Zemun
Abstract
Nikolić Z., S. Vasiljević, Đ. Karagić, M. Vujaković, D.Jovičić, S.Katić and G. Šurlan Momirović (2010): Genetic diversity of red clover cultivars (Trifolium pratense L.) based on protein polimorphism- Genetika, Vol 42, No. 2, 249 -258.
Red clover is the second most important perennial forage legume. Based on morphological characters alone, it is difficult to distinguish accessories of red clover from each other because they have overlapping variations in terms of the major delimiting morphological and biological characters. The aim of this study was to analyze the genetic relationships of 32 red clover cultivars collected from European gene banks, as well as created in Serbia, based on seed storage proteins and isozymes.
From 8 analyzed enzymic systems, there were no differences in zymograms for six enzymes only two enzymes were polymorphic. Two different allelic variants were found for enzyme shikimate dehydrogenase and three for phosphohexose isomeraze.
The seed proteins in the area of higher molecular high weight, 55-95 kDa, were identified as polimorphic. All the cultivars were placed into three clusters on the basis of Ward's distance range. The cluster pattern showed correlation between genetic diversity and geographic origin.
Genetic distance between cultivars based on protein fingerprint could be used as a tool to control and protect intellectual property right over plant breeding material.
Key words: breeding, genotypes, popping maize, sweet maize, white-seeded maize
Corresponding author: Zorica Nikolić, Institute of Filed and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Tel: + 381 21 4898 154; Fax: + 381 21 421 249; e-mail: nikolicz@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 259 -266
© 2010 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.4
Original scientific paper
MULTIVARIATE ANALYSIS FOR HEAD WEIGHT AND YIELD PERFORMANCE OF EXPERIMENTAL CABBAGE HYBRIDS (Brassica oleracea var. capitata L.)
Janko ČERVENSKI, Jelica GVOZDANOVIĆ-VARGA, Mirjana VASIĆ,
Svetlana GLOGOVAC
Institute of field and vegetable crops Novi Sad, Novi Sad, Serbia
Abstract
Červenski J., J. Gvozdenović Varga, M. Vasić, and S.Glogovac (2010): Multivariate analysis for head weight and yield performance of experimental cabbage hybrids (Brassica oleracea var. capitata L.).- Genetika, Vol 42, No. 2, 259 -266.
This paper reviews characters of 18 cabbage genotypes (9 experimental hybrids vs 4 cultivars and 5 hybrids from the domestic commercial production). The experimental genotypes differed in head weight, growing season and yield performance. The analysis of variability of the characters was performed by the PCA method. The two characters selected by the scree test accounted for 57.7% of the variability. Based on head weight and yield performance, the tested hybrids were classified into two clusters that differed quantitatively.
Key words: experimental hybrids, clustering, cabbage, PCA analysis
Corresponding author: Janko Červenski, Institute of Field and Vegetable crops Novi Sad, M.Gorkog 30, 21000 Novi Sad, Serbia, phone + 381 21 4898356, e-mail: jankic@ifvcns.ns.ac.rs, web: www.nsseme.com
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 267 -278
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
kernel modifications and tryptophan content in QPM segregating generations
Dragana IGNJATOVIĆ-MICIĆ, Goran STANKOVIĆ, Ksenija MARKOVIĆ, Snežana MLADENOVIĆ DRINIĆ, Vesna LAZIĆ-JANČIĆ, and Miloje DENIĆ
Maize Research Institute „Zemun Polje“, Belgrade, Serbia
Abstract
Micić Ignjatović D., G. Stanković, K. Marković, S. Mladenović Drinić, V. Lazić Jančić and M. Denić (2010): Kernel modifications and tryptophan content in QPM segregating generations.- Genetika, Vol 42, No. 2, 267 -278.
Maize has poor nutritional value due to deficiency of two essential amino acids – tryptophan and lysine. Although recessive opaque2 (o2) mutation significantly increases their content in the endosperm, incorporation of opaque2 into high yielding cultivars was not commercially successful, because of its numerous agronomic and processing problems due to soft endosperm. Quality protein maize - QPM has lately been introduced as opaque2 maize with improved endosperm hardness and improved agronomic traits, but mostly within tropical and subtropical germplasm. The ongoing breeding project at MRI includes improvement of MRI opaque2 lines and conversion of standard lines to QPM germplasm. The main selection steps in QPM breeding involve assessing kernel modifications and tryptophan level in each generation. Herein, we present the results of the analysis for these traits on F3 and BC1F1 generations of QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses. The results showed that the majority the genotypes had kernel types 2 and 3 (good modifications). The whole grain tryptophan content in F3 and BC1F1 genotypes of crosses between QPM and opaque2 germplasm was at the quality protein level, with a few exceptions. All BC1F1 genotypes of standard lines x QPM had tryptophan content in the range of normal maize, while majority of F3 genotypes had tryptophan content at level of QPM. The progeny (with increased tryptophan levels) of QPM and opaque2 crosses had significantly higher tryptophan content compared to the progeny of crosses between standard and QPM lines – 0.098 to 0.114 and 0.080, respectively. All genotypes that had poorly modified kernels and/or low tryptophan content will be discarded from further breeding.
Key words: kernel modifications, maize, opaque2, QPM, tryptophan content
Corresponding author: Dragana Ignjatović-Micić,,Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade,Serbia,,Tel: 011/3756704,
Fax: 011/3756707,e-mail: idragana@mrizp.rs
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 279 -286
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
EFFECT OF NITROGEN RATE ON GRAIN YIELD OF BREAD WHEAT GENOTYPES
Biljana GORJANOVIĆ 1*, Miroslav ZORIĆ 2, Marija KRALJEVIĆ-BALALIĆ1
1Faculty of agricultural, Novi Sad, Serbia
Abstract
Gorjanović B., M. Zorić, and M. Kraljević Balalić (2010): Effect of nitrogen rate on grain yield of bread wheat genotypes .- Genetika, Vol 42, No. 2, 279 -286.
The improvement in grain yield is the main objective of bread wheat breeding programs. Numerous studies indicate that nitrogen is the key factor of yield and quality in the wheat. The goal of this paper is to investigate variability of grain yield, of twelve bread wheat genotypes, on three nitrogen level. ANOVA showed that this trait was mostly under influence of the genotype (36, 3%), year × genotype interaction (26, 3%), year of investigation (14, 1%), and in the smallest amount of the nitrogen rate (8, 8%). On all three nitrogen level, the highest grain yield was found in the variety Malyska. The lowest grain yield in control was found in the variety Nevesinjka, while in the N75 and N100 rates it was found in the variety Tamaro. The mean performance of individual cultivars, in nine environments (three years × three nitrogen rates), was depicted using which-won-were view of SREG2 biplot. The nine environments fall into two sectors, which is an indication of a strong crossover GE interaction. Genotype Malyska was the winner (the highest yielding variety) in first sector containing seven environments, while genotypes Pertrana and Axis were the winners in second sector containing two environments.
Key words: grain yield, nitrogen, sites regression (SREG) model, wheat
Corresponding author: Gorjanović Biljana, Dušana Jerkovića 22, 22320 Inđija, 065 8432167, e-mail: lazzaric@ptt.rs
Published in „GENETIKA“ Vol. 42, No.2 (2010), pp. 287 -297
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.71
Original scientific paper
IDENTIFICATION OF DONOR LINES CONTAINING FAVORABLE ALLELES FOR QUANTITATIVE TRAITS IN BURLEY TOBACCO (NICOTIANA TABACUM L.)
Slobodan DRAZIC1, Slaven PRODANOVIC2 and Tomislav ZIVANOVIC2
1Institute for Medicinal Plant Research ''Dr J. Pancic'', Belgrade, Serbia
Abstract
Dražić S., S. Prodanović, and T. Živanović (2010): Identification of donor lines containing favorable alleles for quantitative traits in burley tobacco (Nicotiana tabacum L.)- Genetika, Vol 42, No. 2, 287 -297.
Well-adapted local lines might be used as donors to improve existing commercial hybrids. The objective of this study was to identify the best burley tobacco (Nicotiana tabacum L.) line among a group of four lines (SA 130, Bols 334, Barlej T and TN 90) from germplasm collection, for improving an elite single hybrid Bols 335 x TN 86. Values of three quantitative traits (number of leaves, total leaf area and leaf yield) were measured in two-year field experiments. Evaluation of donor lines as sources of new favorable alleles not present in the elite hybrid parent lines was conducted. According to Dudley (1984, 1987) the most significant class for improving quantitative traits is locus class G. Donor line TN 90 had positive values of the parameter μG for number of leaves and for total leaf area. Studied donor lines could not be use directly as the source of new alleles for the improvement of leaf yield in elite hybrid. The improvement of leaf yield in tobacco hybrid Bols 335 x TN 86 would be conducted indirectly via back crossing (TN 86 x TN 90) x TN 86. Significance of GxE interaction for studied traits indicates the same breeding method would be effective in broad range of environmental conditions.
Key words: donor, favorable alleles, locus class, quantitative traits, tobacco, yield component
Corresponding author: Slobodan Dražić, Institute for Medicinal Plant Research ''Dr J. Pancic'', T.Košćuška 1,Belgrade, Serbia, e-mail: sdrazic@mocbilja.rs
Published in „GENETIKA“ Vol. 42, No.2 (2010), pp. 299-306
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.23
Original scientific paper
PROMISING SOUR CHERRY HYBRIDS (Prunus cerasus L.) DEVELOPED
AT FRUIT RESEARCH INSTITUTE ČAČAK
Sanja RADIČEVIĆ, Radosav CEROVIĆ, Ivana GLIŠIĆ,
and Žaklina KARAKLAJIĆ-STAJIĆ
Fruit Research Institute, Čačak, Serbia
Abstract
Radičević S., R. Cerović, I. Glišić and Ž. Karakljajić - Stajić (2010): Promising sour cherry hybrids (Orunus cerasus L.) developed at Fruit Research Institute Čačak - Genetika, Vol 42, No. 2, 299-306.
At Fruit Research Institute in Čačak, major objectives of the work on breeding new sour cherry (Prunus cerasus L.) cultivars are high cropping, large, high-quality fruits and resistance to causal agents of diseases and pests. As a result of the planned hybridization, more than 10,000 hybrid seedlings have been developed from about 40 cultivars within more than 110 parental combinations, among which are ‘Čačanski rubin’ (‘Shasse Morello’ x ‘Köröser Weichsel’) and ‘Šumadinka’ (‘Köröser Weichsel’ x ‘Heimanns Konserven Weichsel’) which have been named and released. Ten-year study of 11 hybrids, selected from the population of about 3,000 hybrid seedlings, gave four hybrids which have been singled out as elite (III/23, III/31, II/40 i XII/57). These hybrids are currently under procedure of being released as new cultivars. The paper presents two-year results of the study of ripening time, pomological properties, biochemical composition of fruits, and field resistance to causal agents of diseases and pests attacking the above named genotypes which were compared to standard cultivar ‘Heimanns Konserven Weichsel’. In the studied hybrids, fruit weight, soluble solids content and sugars content were higher than in standard cultivar. In addition, they exhibit substantial field resistance to causal agents of brown rot (Monilinia laxa /Ader et Ruhl./ Honey ex Whetz.), cherry leaf spot (Blumeriella jaapii (Rehm.) v. Arx.), shot-hole (Clasterosporium carpophilum (Lév.) Aderh.) and cherry fruit fly (Rhagoletis cerasi L.) attack.
Key words: promising hybrids, pomo-technological properties, resistance sour cherry
Corresponding author: Sanja Radičević, Fruit Research Institute,Kralja Petra I 9,32000 Čačak, Serbia,Phone: 032 221 413, Fax: 032 221 391,e-mail: sanjaradicevic@yahoo.com
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 307 - 312
© 2010 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:578
Original scientific paper
GENETIC BASE OF DURABLE RESISTANCE TO Puccinia triticina OF TWO SERBIAN VARIETIES
Željana PRIJIĆ1 and Zoran JERKOVIĆ2
1Faculty of Bio-farming , Bačka Topola
2Institute of Field and Vegetable Crops, Novi Sad
Abstract
Prijić Ž. and Z.Jerković (2010): Genetic base of durable resistance to Puccinia triticina of two Serbian varieties.- Genetika, Vol 42, No. 2, 307 - 312.
The aim of the study was to differentiate the two long term incomplete resistant varieties according to Lr genes and compare the effects of some in the accumulation. The investigated varieties were developed more than 15 years ago. Basis of the trait was investigated according to reaction type (RT) 10 days after spores of the race 2 were trashed at seedlings of F2 generations of varieties Anastasia or Selekta x Lr 1, Lr 2a, Lr 3, Lr 13, Lr 14a, Lr 16 or Lr 26 grown at air temperatures 20-25oC. The presence of single resistant genes Lr 1 and Lr 2a in the varieties was excluded by presence of susceptible plants in F2 progenies of adequate crosses. The RT decreaseable combinations were Lr 3+B as Lr3+C in Selekta and Lr26+E, Lr26+C as EC in Anastasia. According to lower infection efficiency and yellowing of the above tip top part the C was similar or Lr 34. The Lr 13 and 14a were near the same effective at seedlings when were added to proposed combinations in parents. According to F1 results in field the most effective over season combination was achieved in progeny of the investigated varieties because of two LP prolong able complementary genes and accumulation of three infection severity responsible ones.
Key words: durable resistance, latent period, Puccinia triticina, wheat
Corresponding author: Željana Prijić, Faculty of biofarming, Maršala Tita 39, 24300 Bačka Topola, email: zeljana.prijic@yahoo.com
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 313 - 322
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
VISUAL ASSESSMENT OF SWEET MAIZE LINES PHENOTYPE, ACCORDING TO UPOV DESCRIPTOR, AS INDICATOR OF HETEROSIS
Vojka Babić1, Zorica Pajić1, Slaven Prodanović2,
Milosav Babić1 and Milomir Filipović1
1 Maize Research Institute, Zemun Polje, Belgrade
2Faculty of Agriculture, University of Belgrade, Belgrade
Abstract
Babić V., Z. Pajić, S. Prodanović, M.Babić and M. Filipović (2010): Visual assessment of sweet maize lines phenotype, according to UPOV descriptor, as indicator of heterosis- Genetika, Vol 42, No. 2, 313 - 322.
The organisation of germplasm into genetically divergent groups is of extreme importance for the development of hybrid varieties in which the identification and exploitation of heterosis is very important for the final result of breeding. It can also be beneficial for breeding of self-pollination varieties, clones and synthetics. The discovery of heterotic groups in standard grain quality maize significantly improved the process of testing. The improvement in sweet maize was relatively modest in comparison to standard grain quality maize due to a relatively narrow genetic base of sweet maize, then poorly defined heterotic relations, scarce sources of germplasm that satisfy commercial standards, poor seed vigour, low quality of root and stalk, as well as, a short period of time for the estimation of yield and quality.
The efficiency of hybrid breeding programmes would be significantly higher if heterosis could be predicted prior to the evaluation in the field. The application of the multivariate analysis method to data of phenotypic characterisation according to the UPOV Descriptor was an attempt to establish whether such a procedure can be beneficial for the determination of related groups and whether the phenotypic distance, obtained on the basis of a visual estimation of a plant group, can be an indicator of heterosis. Obtained results indicate that clustering is highly analogous to the pedigree. Since sweet maize breeders have at their disposal less significant genetic variability and search for the development and defining of heterotic groups, as such a procedure can be useful in both, the process of the hybrids development and the process of new inbreed development and genetic variability increasing. Certainly, further systematic studies aimed at this direction are necessary to reliably ascertain that the phenotypic distance is a justifiable indicator of heterosis.
Key words: heterosis, phenotypic distance, sweet maize
Corresponding author: Babic Vojka, Maize Research Institute „Zemun Polje“, Slobodana Bajica 1, 11185 Zemun-Belgrade, Serbia; Tel: (011)3756704, Fax: 3756707, e-mail: vbabic@mrizp.rs
Published in „GENETIKA“ Vol. 42, No. 2 (2010), pp. 323 -330
© 2010 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC: 575:633.16
Original scientific paper
PRODUCTION CHARACTERISTICS OF DIFFERENT MALTING BARLEY GENOTYPES IN INTENSIVE NITROGEN FERTILIZATION
Miroslav Malešević1, Đorđe GLAMOČLIJA2, Novo Pržulj1, Vera POPOVIĆ1,
Saša STANKOVIĆ 3, Tomislav ŽIVANOVIĆ2 and Angelina TAPANAROVA2
1Institute of Field and Vegetable Crops, Novi Sad, Serbia
2Faculty of Agriculture, Belgrade, Serbia
3Technological Research Center, Zaječar, Serbia
Abstract
Malešević M., Đ. Glamočlija, N. Pržulj, V.Popović, S.Stanković, T. Živanović and A. Taparanova (2010): Production characteristics of different malting barley genotypes in intensive nitrogen fertilization.- Genetika, Vol 42, No. 2, 323 -330.
Two-year trials have been conducted in Technological Research Center in Zaječar. Research objects were six malting barley genotypes. The experimental crop was top dressed with the following amounts of nitrogen: 40, 60, 80 and 100 kg ha-1. The control variant was not top dressed. The obtained results showed that the genotypes reacted significantly to the increased amounts of nitrogen by changing their production characteristics and seed quality. In addition, the genotypes demonstrated certain varietal differences. A new genotype, Premijum, was highest yielding and it had the lowest total proteins in the grain. The genotype NS-525 had the highest 1000-grain weight (46.8 g). The effect of nitrogen on the studied characteristics depended on N quantity applied. Increasing amounts of nitrogen decrease positive effects on spike length, number of grains per spike, 1000-grain weight and yield. However, the protein content in grain kept increasing to the highest nitrogen dose, which lowered the quality of malting barley. The highest yield was obtained by applying 80 and 100 kg ha-1 of nitrogen, depending on the year of study.
Key words: genotype, malting barley, nitrogen fertilization, morphological characteristic, yield, grain quality.
Corresponding author: Miroslav Malešević, Institute of Field and Vegetable Crops, Novi Sad, Tel: +381 21 4898 481; 064 8205 700, E-mail: malesmir@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 331 - 338
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.10
Original scientific paper
EFFECT OF ‘GUTINGEN V’ AS AN INTENSIVE TRAINING SYSTEM ON AGROMORPHOLOGICAL CHARACTERS OF SOME APPLE CULTIVARS IN KARAJ REGION OF IRAN
Ahmad DADASHPOUR*, Alireza TALAEI and Ali SHAHI-GHARAHLAR
Department of Horticulture, University College of Agriculture & Natural Resources,
University of Tehran, Karaj, 31587, IRAN
Abstract
Dadashpour A., A. Talaei and A. Shahi-Gharahlar (2010): Effect of ‘Gutingen V’ as an intensive training system on agromorphological characters of some apple cultivars in Karaj region of Iran.- Genetika, Vol 42, No. 2, 331 - 338.
V-shape systems represent an efficient and popular option to increase yields and fruit quality in fruit trees. Hence, this paper attempts to compare some vegetative and yield characteristics of five apple cultivars grown at horticultural research station in Karaj, Iran. The selected apple cultivars were included: ‘Golab-kohans’ (Iranian cultivar), ‘Fuji’, ‘Gala’, ‘Starking’ and ‘Delbar estival’ (commercial foreign cultivars) that were grafted on M.9 rootstock which were trained in a V system. All of these trees were planted in winter 2005. The time of irrigation started from the second experimental year after planting and the method of irrigation was drip irrigation system. Results showed that the ‘Golab-kohans’ had the most tree height (278.63 cm), trunk cross sectional area (7.308 cm2) and mean shoot length (100.58 cm). Also ‘Delbar estival’ had the most yield (0.98 kg), yield efficiency (0.550 kg/cm2). Consistently, Results revealed that among the investigated cultivars, ‘Delbar estival’ can be introduced as a prone and productive cultivar for V system in Karaj’s climatic conditions.
Key words: apple, V system, vegetative and reproductive traits.
Corresponding author: Ahmad Dadashpour, Department of Horticulture, University College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587, IRAN, Tel.: +98 9125677083,e-mail: ahmad.dadashpour@gmail.com
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 339 - 348
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
INBRED LINES OF DIFFERENT CYCLES OF SELECTION AS DONORS OF favourable alleles FOR THE IMPROVEMENT OF F1 MAIZE HYBRIDS
1Maize Research Institute “Zemun Polje”, Belgrade-Zemun
2institute for Medicinal Plant Research "Dr Josif Pančić", Belgrade
3Faculty of Agriculture, Belgrade-Zemun
Abstract
Sečanski M., G. Todorović, G. Šurlan Momirović, T.Živanović and M. Babić (2010): Inbred lines of different cycles of selection as donors of favourable alleles for the improvement of F1 maize hybrids.- Genetika, Vol 42, No. 2, 339 - 348.
Eight BSSS and BSCB1 inbred lines of different cycles of selection were studied with the aim to evaluate which inbreds had the highest relative values of favourable alleles for the improvement of the grain yield trait in an elite single cross maize hybrid. Based on estimated parameters mG, UBND, PTC and NI it was determined that the inbreds B73 (C5) and B84 (C7), originating form the synthetic population BSSS had highest number of favourable alleles for the improvement of the grain yield. Both these inbreds belong to the later cycles of recurrent selections and proved to be better donors of favourable alleles than inbreds belonging to the earlier cycles of selection. The rank correlations of used parameters were positive and highly significant, while the highest values of correlations for yield were determined between mG and NI, that is PTC and NI. The highest grain yield was recorded in the hybrid ZPL2 x B73, which also had the highest value of heterosis (174.9%) in the trial for estimation of loci relative values.
Key words: donor of favourable alleles, heterosis, .inbred line, maize, rank correlation
Corresponding author: dr Mile Sečanski, Maize Research Institute “Zemun Polje”, S.Bajića 1, 11185 Belgrade-Zemun
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 349 - 358
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.3
Original scientific paper
THE GENOTYPE TRAITS OF FORAGE SORGHUM, SUDAN GRASS AND THEIR INTERSPECIES HYBRID IN THE CONDITIONS OF INTENSIVE NITROGEN NUTRITION
Jela IKANOVIC1, Đorđje GLAMOCLIJA1, Radojka MALETIC1,
Snezana JANKOVIC2, Marjenka TABAKOVIĆ3 and Ljubisa Zivanovic1
1Faculty of Agriculture, Belgrade, Serbia
2Institute for Science Application in Agriculture, Belgrade, Serbia
3Maize Institute, Zemun Polje, Serbia
Abstract
Ikanović J., Đ. Glamočlija, R. Maletić, S. Janković, M.Tabaković and Lj. Živanović (2010): The genotype traits of forage sorghum, sudan grass and their interspecies hybrid in the conditions of intensive nitrogen nutrition.- Genetika, Vol 42, No. 2, 349 -358.
The subject of this study is a three-year study (carried out during 2007, 2008 and 2009) of the morphological characteristics variability in three genotypes NS-Džin (Forage Sorghum), Zora (Sudan grass) and Siloking (interspecies hybrid) depending on the amount of nitrogen used for plant nutrition. For the height and mass analysis of fresh stems and leaves as well as leaf mass and leaf portion in the total biomass, samples were taken from the first swath when the effect of the used nitrogen amounts was the greatest. The results have shown that there are significant variations in the tested properties between the genotypes. The Siloking genotype had the highest total biomass during all three years. The largest leaf mass was found in the NS-Džin genotype, while the Zora genotype had the highest leaf portion in the three-year average. The effect of nitrogen use depended on the weather conditions, as well as the layout of the rainfall, so that the optimal quantity was 105 kg ha-1 during the first and the second year, and 150 kg ha -1 during the third year.
Key words: genotype, interspecies hybrid, nitrogen nutrition, sorghum, sudan grass
Corresponding author: Djordje Glamočlija, Faculty of Agriculture, Zemun-Belgrade, Tel: +381 11 2615 315; E-mail: lami@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 359-375
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.10
Original scientific paper
APPLICATION OF MOLECULAR MARKERS IN APPLE BREEDING
Slađana MARIĆ1, Milan LUKIĆ1, Radosav CEROVIĆ1, Milisav MITROVIĆ1
and Radovan BOŠKOVIĆ2
1Fruit Research Institute, Čačak, Serbia
2Division of Biology, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK
Abstract
Marić S., M. Lukić, R. Cerović, M. Mitrović and R. Bošković (2010): Application of molecular markers in apple breeding - Genetika, Vol 42, No. 2, 359-375.
Apple is economically the most important species of genus Malus Miller. In respect of production, trade and consumption, it ranks first among deciduous fruit and third on a global scale among all fruit species. Apple breeding is carried out on a large scale in several scientific institutes throughout the world. Due to this activity, apple is a fruit species with the highest number of described monogenic traits; 76 genes, encoding morphological traits, pest and disease resistance, as well as 69 genes encoding enzymes. The development of molecular markers (RFLPs, AFLPs, SCARs and SSRs) has allowed the mapping of the apple genome and the development of several saturated genetic maps, to which genes controlling important traits are assigned. Markers flanking these genes not only play an important role in selecting parental combinations and seedlings with positive traits, but they are also particularly important in detecting recessive traits, such as seedless fruit. In addition they enable pre-selection for polygenic quantitative traits. In recent years, particular attention has been paid to biochemical and physiological processes involved in the pathway of important traits e.g., ripening and the storage capability of apple fruit.
Key words: apple, marker, genetic map, QTL
Corresponding author: Slađana Marić, Fruit Research Institute, 32000 Čačak, Serbia, email:nidzovicsladja@yahoo.com
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 377-385
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:576.385
Original scientific paper
PREVALENCE OF S AND Z ALPHA 1-ANTITRYPSIN MUTATIONS IN PATIENTS WITH PANCREATIC DISEASES IN SERBIAN POPULATION
Aleksandra NIKOLIC1, Aleksandra DIVAC1, Marija STANKOVIC1, Jelena DINIC1, Snezana LUKIC2, Marina ANDJELIC-JELIC3, Dragan POPOVIC2,
Dragica RADOJKOVIC1
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
2Department of Gastroenterology, Clinical Center of Serbia, Belgrade, Serbia
3Department of Endocrinology, University Clinical Center “Zvezdara”, Belgrade
Abstract
Nikolić A., A. Divac, M. Stanković, J.Dinić, S.Lukić, M.Anđelić-Jelić, D.Popovuć, and D.Radojković (2010): Prevalence of s and z alpha 1-antitrypsin mutations in patients with pancreatic diseases in Serbian population.- Genetika, Vol 42, No. 2, 377-385.
One of the key points in research of pancreatic disease pathology is further elucidation of the role of proteases and antiproteases, since their imbalance can lead to pancreatic injury. Alpha 1-antitrypsin (AAT) is one of the most important serum inhibitors of proteolytic enzymes, including pancreatic enzymes trypsin, chymotrypsin and elastase. It is speculated that mutations in the AAT gene may influence the onset and the development of pancreatic disease. The presence of the most common AAT mutations Z and S was analyzed in 160 patients with pancreatic diseases (50 patients with pancreatic cancer, 50 patients with chronic pancreatitis and 60 patients with type 2 diabetes mellitus) and 129 healthy individuals by PCR-mediated site-directed mutagenesis (PSM) method. One patient with pancreatic cancer was found to be a carrier of Z mutation, as well as one patient with type 2 diabetes mellitus. One patient with chronic pancreatitis was found to be a carrier of S mutation. The common AAT mutations were statistically significantly over-represented in patients with pancreatic diseases (3 of 160 patients, allelic frequency 0.9%) than in the control group (1 of 129 individuals, allelic frequency 0.4%). The results of this study, requiring confirmation, suggest that common AAT mutations Z and S may be associated with a modest increase in susceptibility to the development of pancreatic disease.
Key words: alpha 1-anitripsin, pancreatic cancer, chronic pancreatitis, diabetes mellitus type 2, Z and S mutations
.
Corresponding author: Aleksandra Divac, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, PO Box 23,11010 Belgrade,Serbia,Tel: +381 11 3976658,Fax: +381 11 3975808,E-mail: aleksandradivac@imgge.bg.ac.rs
Published in „GENETIKA“ Vol. 42, No. 2(2010), pp. 387-396.
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.22
Original scientific paper
EXAMINING SELF-COMPATIBILITY IN PLUM (Prunus domestica L.)
BY FLUORESCENCE MICROSCOPY
Dragan NIKOLIĆ and Dragan MILATOVIĆ
Faculty of Agriculture, Belgrade, Serbia
Abstract
Nikolić D., and D. Milatović (2010): Examining self-compatibility in plum (Prunus domestica L.) by fluorescence microscopy.- Genetika, Vol 42, No. 2, 387-396.
Self-compatibility in 18 European plum cultivars was examined using the method of fluorescence microscopy. According to self-compatibility, cultivars were divided into two groups: self-compatible and self-incompatible. In self-compatible cultivars the number of pistils, where pollen tubes reached the base of the style varied from 32.00% (Anna Späth) to 91.18% (Wangenheims Frühzwetsche). Mean number of pollen tubes at the base of style in these cultivars ranged from 0.52 to 3.97. Cultivars were considered self-incompatible if pollen tubes stopped their growth in the style along with forming characteristic swellings at their tips. Of the studied cultivars, 13 were found to be self-compatible: Wangenheims Frühzwetsche, Čačanska Lepotica, Valjevka, California Blue, Čačanska Rodna, Italian Prune, Stanley, Požegača, Herman, Bluefre, Jelica, Ruth Gerstetter and Anna Späth, while 5 were found to be self-incompatible: Čačanska Rana, Zimmers Frühzwetsche, Čačanska Najbolja, Pacific and President.
Key words: plum, pollen tube growth, self-compatibility
Corresponding author: Dragan Nikolić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, e-mail: nikolicd@agrif.bg.ac.rs