GENETIKA, Vol. 46, No. 1 (2014)

Vanya KOLEVA, Asya DRAGOEVA, Marian DRAGANOV, Laura MELENDEZ-ALAFORT, Antonio ROSATO, Nikolay UZUNOV, Dobromir ENCHEV

INHIBITION OF GROWTH AND INDUCTION OF APOPTOSIS IN HUMAN LUNG CANCER CELLS BY BR-OXPH [Abstract] [Full text]

Dragan MILIĆ, Đura KARAGIĆ, Sanja VASILJEVIĆ, Aleksandar MIKIĆ, Branko MILOŠEVIĆ, Slobodan KATIĆ

BREEDING AND IMPROVEMENT OF QUALITY TRAITS IN ALFALFA (Medicago sativa ssp. sativa L.) [Abstract] [Full text]

Nikolay PANAYOTOV and Dochka DIMOVA

ASSESSMENT OF YIELD AND YIELD STABILITY OF NEW PERSPECTIVE PEPPER BREEDING LINES WITH CONICAL SHAPE [Abstract] [Full text]

Desimir KNEŽEVIC, Sonja MARIĆ, Vlado KOVAČEVIĆ, Nevena DJUKIĆ, Srdjan ATANASIJEVIC

VARIABILITY OF GENOTYPE AND INHERITANCE OF GRAIN NITROGEN CONTENT IN WINTER WHEAT (Triticum aestivum L.) [Abstract] [Full text]

Eleonora ČAPELJA, Nevena STEVIĆ, Vladislava GALOVIĆ, Milana NOVAKOVIĆ, Maja KARAMAN

rDNA BASED ANALYSIS OF AUTOCHTONOUS FUNGAL SPECIES FROM SERBIA [Abstract] [Full text]

Ahmad Reza GOLPARVAR

GENETIC CONTROL AND HEREDITY OF HARVEST INDEX AND BIOLOGICAL YIELD IN BREAD WHEAT (Triticum aestivum L.) [Abstract] [Full text]

Milomir FILIPOVIĆ, Milosav BABIĆ, Nenad Delić, Goran Bekavac, Vojka BABIĆ

DETERMINATION relevant breeding criteria by the path AND factor analysis IN MAIZE [Abstract] [Full text]

Novo PRŽULJ, Vojislava MOMČILOVIC, Jasmina SIMIĆ, Milan MIROSAVLJEVIĆ

EFFECT OF GROWING SEASON AND VARIETY ON QUALITY OF SPRING TWO-ROWED BARLEY [Abstract] [Full text]

Wen JIN, Qingqiong DENG, Meiying NIU, Liping HU, Aijuan LIU, Guihua TIAN, Jianqiu LU, Mingquan ZHOU, Ghulam MURTAZA, Haiyan JIANG

EFFECTIVE CHEMICAL CONSTITUENT’S CONFIRMATION OF EXTRACTING SOLUTION FROM HERBS AND THEIR GENE TARGETS [Abstract] [Full text]

Mladen POPOVAC, Milica PETROVIĆ, Dragan RADOJKOVIĆ, Dragan STANOJEVIĆ, Aleksandar MILETIĆ, Predrag PERIŠIĆ

THE ASSESSMENT OF GENETIC POTENTIAL IN PERFORMANCE TESTED GILTS BY MEANS OF SELECTION INDEXES METHOD [Abstract] [Full text]

Jela Ikanović, Vera Popović, Snežana Janković, Ljubiša Živanović, Sveto Rakić, Dalibor Dončić

Khorasan WHEAT POPULATION RESEARCHING (Triticum turgidum, SSP. TURANICUM (McKEY) IN THE MINIMUM TILLAGE CONDITIONS [Abstract] [Full text]

Vladan POPOVIĆ, Aleksandar LUČIĆ, Mirjana ŠIJAČIĆ-NIKOLIĆ, Tatjana ĆIRKOVIĆ-MITROVIĆ, Ljubinko RAKONJAC, Branislav CVJETKOVIĆ, Snežana MLADENOVIĆ DRINIĆ

ANALYSIS OF INTER-LINE VARIABILITY OF BALD CYPRESS (Taxodium distichum L. Rich.) JUVENILE SEEDLINGS using morphometric markers [Abstract] [Full text]

Hilal YILDIZ, Sezai ERCISLI, Haluk Kemal NARMANLIOGLU, Saban GUCLU, Mustafa AKBULUT, Zumrut TURKOGLU

THE MAIN QUALITY ATTRIBUTES OF NON-SPRAYED CHERRY LAUREL (Laurocerasus officinalis Roem.) GENOTYPES [Abstract] [Full text]

Dušica JOVIČIĆ, Zorica NIKOLIĆ, Miroslav ZORIĆ, Ana MARJANOVIĆ-JEROMELA, Gordana PETROVIĆ, Dragana MILOŠEVIĆ, Maja IGNJATOV

VIABILITY OF OILSEED RAPE (Brassica napus L.) SEEDS UNDER SALT STRESS [Abstract] [Full text]

Eva Kluzakova, Roman Stupka, Michal Sprysl, Jaroslav Citek,Monika Okrouhla

THE INFLUENCE OF CAST/MspI, HinfI, RsaI POLYMORPHISM ON PRODUCTION TRAITS IN PIGS [Abstract] [Full text]

Nada Hladni, Vladimir Miklič, Siniša Jocić, Marija Kraljević-Balalić, Dragan Škorić

Mode of inheritance and Combining ability for plant height and head diameter in sunflower (Helianthus annuus L.) [Abstract] [Full text]

Dušan Dj. KOVAČEVIĆ, Svetlana ROLJEVIĆ, Željko DOLIJANOVIĆ, Snežana DJORDJEVIĆ, Vesna MILIĆ

Different genotypes of alternative small grains in organic farming [Abstract] [Full text]

Ana MARJANOVIĆ JEROMELA, Radovan MARINKOVIĆ,Milan JOCKOVIĆ, Petar MITROVIĆ, Željko MILOVAC, Nikola HRISTOV, Jasna SAVIĆ,Biljana STAMENKOVIĆ

EVALUATION OF GENETIC VARIANCE COMPONENTS FOR SOME QUANTITATIVE TRAITS IN RAPESEED (Brassica napus L.) [Abstract] [Full text]

Mehrzad ALLHGHOLIPOUR, Ezatollah FARSHDFAR, Babak RABIEI

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS OF DIFFERENT RICE CULTIVARS BY MICROSATELLITE MARKERS [Abstract] [Full text]

Emina Mladenović, Janoš Berenji, Vladislav Ognjanov, Mirjana Ljubojević, Jelena Čukanović, Tihomir salamun

Genetic diversity in a collection of ornamental squash (Cucurbita pepo L.) [Abstract] [Full text]

Lejla KAPUR-POJSKIĆ, Naris POJSKIĆ, Gabrijela RADOSAVLJEVIĆ, Damir MARJANOVIĆ, Rifat HADŽISELIMOVIĆ

MtDNA VARIATION WITHIN LOCAL HUMAN POPULATIONS IN BOSNIA AND HERZEGOVINA [Abstract] [Full text]

Ivan Pihler, Hrisula Kiprijanovska, Nada Plavša, Milan Krajinović, Aleksandar Uzunov, Per KRYGER, Nebojša Nedić

POPULATION-GENETICAL CHARACTERISTICS OF THE BEE POPULATION OF VOJVODINA [Abstract] [Full text]

SANJAY KUMAR and A. K. SINGH

COMPLETE ABSENCE OF LINKAGE DISEQUILIBRIUM BETWEEN ENZYME LOCI IN NATURAL POPULATIONS OF Drosophila ananassae [Abstract] [Full text]

Nevena STEVIĆ, Eleonora ČAPELJA, Vladislava GALOVIĆ, Milana NOVAKOVIĆ, Maja KARAMAN

MOLECULAR CHARACTERISATION OF SOME LIGNICOLOUS SPECIES FROM FUNGAL CULTURE COLLECTION [Abstract] [Full text]

Marko JOSIPOVIC, Hrvoje PLAVSIC, Vlado KOVACEVIC,Monika MARKOVIC, Dario ILJKIC

IMPACTS OF IRRIGATION AND GENOTYPE ON YIELD, PROTEIN, STARCH AND OIL CONTENTS IN GRAIN OF MAIZE INBRED LINES [Abstract] [Full text]

Nataša KOČIŠ TUBIĆ, Mihajla DJAN, Nevena VELIČKOVIĆ, Goran ANAČKOV, Dragana OBREHT

GRADUAL LOSS OF GENETIC DIVERSITY OF Ambrosia artemisiifolia L. POPULATIONS IN THE INVADED RANGE OF CENTRAL SERBIA [Abstract] [Full text]

Fereshteh ASHRAFI, Ali HASHEMI, Karim MARDANI, Reza DARVISHZADEH

STUDY ON GENETIC VARIABILITY IN MHC-DRB1 SECOND EXON IN MAKUIE SHEEP BREED POPULATION [Abstract] [Full text]

Gordana ŠEBEK, Višnja KOVAČEVIĆ

COMPATIBILITY AND VERDURE OF SEEDLING FOR AUTOCHTONOUS PEER IN THE REGION OF NORTH MONTENEGRO [Abstract] [Full text]

Biljana BUFAN, Jasmina DJIKIĆ, Mirjana NACKA-ALEKSIĆ, Zorica STOJIĆ-VUKANIĆ, Mirjana DIMITRIJEVIĆ, Gordana LEPOSAVIĆ

STRAIN-SPECIFIC DIFFERENCES IN AGE-RELATED CHANGES IN RAT SUSCEPTIBILITY TO EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AND DENDRITIC CELL CYTOKINE GENE EXPRESSION [Abstract] [Full text]

Aleksandra SAVIĆ, Gordana PETROVIĆ, Mirjana MILOŠEVIĆ, Zorica NIKOLIĆ, Anamarija STOJANOVIĆ, Jelica GVOZDANOVIĆ-VARGA, Vida TODOROVIĆ, MirjanaVASIĆ

Morpho-chemical characterization of dry and snap bean (Phaseolus vulgaris L.) landraces collected on Fruška gora Mt. [Abstract] [Full text]

Bosiljka PLEĆAŠ-SOLAROVIĆ, Ninoslav ĐELIĆ, Vladan BAJIĆ, Lada ŽIVKOVIĆ, Biljana SPREMO-POTPAREVIĆ

CYTOGENETIC ALTERATIONS IN PERIPHERAL CELLS OF ALZHEIMER’S DISEASE PATIENTS [Abstract] [Full text]

Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp. 1-10

UDC 575
DOI: 10.2298/GENSR1401001K

Original scientific paper

INHIBITION OF GROWTH AND INDUCTION OF APOPTOSIS IN HUMAN LUNG CANCER CELLS BY BR-OXPH

Vanya KOLEVA¹*, Asya DRAGOEVA¹, Marian DRAGANOV², Laura MELENDEZ-ALAFORT³, Antonio ROSATO³, Nikolay UZUNOV¹ and Dobromir ENCHEV¹

¹ Faculty of Natural Sciences, University of Shumen, Bulgaria

² Medical University of Plovdiv, Bulgaria

³ Istituto Oncologico Veneto IRCCS, Padova, Italy

Abstract

Koleva V., A. Dragoeva, M. Draganov, L. Melendez-Alafort, A. Rosato, N.Uzunov and D. Enchev (2014): Inhibition of growth and induction of apoptosis in human lung cancer cells by Br-oxph- Genetika, vol., No.46, No.1, 1-10.

The study was aimed at evaluating apoptotic potential of Br-oxph (4-bromo-N,N-diethyl-5,5-dimethyl-2,5-dihydro-1,2-oxaphosphol-2-amine 2-oxide) in vitro. The dose response effect of Br-oxph (dose range 1-3 mg/ml, for 48 h) on SK-MES-1 cells viability was determined by means of WST-1 cell proliferation assay. The half maximal inhibitory concentration (ІС50) value was determined – 1.8 mg/ml. The ability of the compound tested to induce apoptosis was tested by ELISA to detect cellular DNA fragmentation. We provided a quantitative assessment of the apoptotic potential of Br-oxph in human lung carcinoma cells at concentrations corresponding to ІС50 and 2хІС50 for 3 hours. Treatment with 2хІС50 significantly increased the amount of cytoplasmic DNA-fragments. Results obtained from the present study confirm that Br-oxph target the cancerous cells towards apoptosis.

Key words: Apoptosis, Br-oxph, DNA fragmentation, cancer cell line, cell viability

Corresponding author: Vanya Koleva, Faculty of Natural Sciences, University of Shumen, str. "Universitetska" 115, 9712 Shumen, Bulgaria, fax: +359 54 830 371; phone: +359 54 830 495; e-mail: vanyakolleva@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp. 11 -18

Serbia

UDC 575:633.3
DOI:10.2298/GENSR1401011M

Original scientific paper

BREEDING AND IMPROVEMENT OF QUALITY TRAITS IN ALFALFA (Medicago sativa ssp. sativa L.)

Dragan MILIĆ, Đura KARAGIĆ, Sanja VASILJEVIĆ, Aleksandar MIKIĆ, Branko MILOŠEVIĆ,

and Slobodan KATIĆ

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Milić D., Đ. Karagić, S. Vasiljević, A. Mikić, B. Milošević, and S. Katić (2014): Breeding and improvement of quality traits in alfalfa (Medicago sativa ssp. sativa L.) -.Genetika, Vol 46, No. 1, 11-18.

An important objective in alfalfa breeding is the improvement of forage quality. The aim of this experiment was to determine the genetic control of the most important quality traits in whole plant leaves and stems of alfalfa genotypes. Quantitative genetic parameters were estimated for crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) content. A 5x5 diallel design with reciprocals among parents from geographically different populations were studied. In the diallel study, effects due to general combining ability (GCA) were higher than those due to specific combining ability (SCA) for majority of the tested characters in analyses of whole plant. The F1 progeny effect was significant for almost all characters (except ADF content). The highest values of narrow sense heritability’s were found for NDF (h =0.19) and CP (h =0.17) in whole plant, and in leaves (0.11 and 0.08), while in stems most heritable traits are ADF (h = 0.29) and ADL (h = 0.16) content. As a consequence, CP, NDF and ADF content would be more efficient selection criteria in whole plant analyses, while ADF and ADL content would be more interesting to investigate in stems, in a breeding program for improved nutritive value of alfalfa.

Key words: alfalfa, diallel, GCA, heritability, SCA, variance

Corresponding author: Dragan Milić, Forage Crops Department, Institute of Field and Vegetable Crops M. Gorkog 30. 21000 Novi Sad, Serbia tel.: + 381 21 4898376; fax: + 381 21 4898377, e-mail: dragan.milic@nsseme.com

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Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp. 19- 26

Serbia

UDC 575:635
DOI: 10.2298/GENSR1401019P

Original scientific paper

ASSESSMENT OF YIELD AND YIELD STABILITY OF NEW PERSPECTIVE PEPPER BREEDING LINES WITH CONICAL SHAPE

Nikolay PANAYOTOV and Dochka DIMOVA

Agricultural University, Plovdiv, Bulgaria

Abstract

Panayotov N., and D. Dimova (2014): Assessment of yield and yield stability of new perspective pepper breeding lines with conical shape .-Genetika, Vol 46, No. 1, 19-26.

The main goal of the present study was to estimate new perspective Bulgarian conic lines pepper about yield and stability of the yield with purpose to be presented for Government variety testing and to been included in new breeding programs. Three years experiments were carried out in Experimental fields of Agricultural University, Plovdiv, Bulgaria with eight new perspective consolidated hybrids of sweet pepper. Typical Bulgarian variety Kurtovska kapia 1619 was used as a standard. The plants were grown according to the conventional technology for middle early field production for South Bulgarian condition. Four harvests were done – one of green fruits and three of red ripening fruits. The total yield, weight and length of fruit were determined. The stability of yield (Ysi) by the methods Kang was calculated. The lines with the highest yield were Doux Marconi Rouge х Kapia 1300 F₈and Doux Marconi San Semences х Kalinkov 800/7 F₇with productivity of 5034.1 kg/da and 4881.3 kg/da respectively. The index of yield stability was highest 7+ about following breeding lines Doux Marconi San Semences х Kalinkov 800/7 F₇ and Kapia 1300 х Doux d’Espagne F₉. The lines Doux Marconi Rouge х Kapia 1300 F₈, Doux Marconi San Semences х Kalinkov 800/7 F₇ and Doux Marconi Rouge х Kapia 1300 F₇ are suitable to be included in new selection programs for more successfully breeding.

Key words: adaptability, index of stability, Kang (YSi), productivity, selection

Corresponding author: Nikolay Panayotov, Agricultural University, 12 “Mendeleev” Str. 4000 Plovdiv, Bulgaria, Tel.: +35932654257, Mobile tel.: +359887872720, fax:+35932633157, e-mail: nikpan@au-plovdiv.bg

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Published in „GENETIKA“ Vol. 46, No.1 (2014), pp. 27 -32

Serbia

UDC 575:633.11
DOI: 10.2298/GENSR1401027K

Original scientific paper

VARIABILITY OF GENOTYPE AND INHERITANCE OF GRAIN NITROGEN CONTENT IN WINTER WHEAT (Triticum aestivum L.)

Desimir KNEŽEVIĆ¹, Sonja MARIĆ², Vlado KOVAČEVIĆ²,

Nevena DJUKIĆ³, Srdjan ATANASIJEVIC⁴

¹University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lesak, Kosovo and Metohia, Serbia

²University J. J. Strossmayer in Osijek, Faculty of Agriculture, Osijek, Croatia

³University of Kragujevac, Faculty of Natural Science, Department of Biology,

Kragujevac, Serbia

⁴Technical College of Professional Studies, in Kragujevac, Department of Informatics, Kragujevac, Serbia

Abstract

Knežević D., S. Marić, V. Kovačević, N. Đukić, and S. Atanasijević (2014): Variability of genotype and inheritance of grain nitrogen content in winter wheat (Triticum aestivum L.)-. Genetika, Vol 46, No. 1, 27-32.

In diallel crosses (without reciprocals) of four genetically divergent wheat cultivars (Yugoslavia, Osijecanka, Zitnica, and NS Rana 2) the variability and inheritance of nitrogen content in wheat plant were investigated. The mode of inheritance, gene effect, heritability in parent cultivars and F₂ hybrids were studied. The different mode of inheritance: partial dominance, intermediate and overdominance for analyzed trait was established. Among parent cultivars, the highest nitrogen contents had Novosadska Rana 2 (3.52%). The combination Zitnica/NS Rana 2 in F₂ grain generation was the best for nitrogen content.

Key words: cultivars, hybrids, inheritance, nitrogen, wheat

Corresponding author: Desimir Knežević-Faculty of Agriculture, University of Priština, Kosovska Mitrovica, Lesak 38219, Kopaonicka bb., Kosovo and Metohija, Serbia, Phone: +381 64 614 8882 e-mail deskoa@ptt.rs

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Published in „GENETIKA“ Vol. 46, No.1 (2014), pp. 33 -42

Serbia

UDC 575:630
DOI: 10.2298/GENSR1401033C

Original scientific paper

rDNA BASED ANALYSIS OF AUTOCHTONOUS FUNGAL SPECIES FROM SERBIA

Eleonora ČAPELJA¹, Nevena STEVIĆ¹, Vladislava GALOVIĆ², Milana NOVAKOVIĆ¹,

Maja KARAMAN¹

¹University of Novi Sad, Faculty of Sciences, Department for Biology and Ecology, Novi Sad, Serbia

²Institute of Lowland Forestry and Environment, Novi Sad, Serbia

Abstract

Čapelja E., N. Stević, V. Galović, M. Novaković, and M. Karaman (2014): rDNA based analysis of autochtonous fungal species from Serbia-. Genetika, Vol 46, No. 1, 33 - 42.

Determination of fungal species by traditional morphological approach can often be problematic. In the phylum Basidiomycota, sporocarps of different species can share very similar morphoanatomical characteristics. Using molecular markers and phylogenetic species concept this problem can be reduced. In this study identification of six autochtonous fungal species, collected from several locations in Serbia (Tara, Kopaonik, Stara planina) was done by comparison between morphological and molecular data of fungal species, as well as information obtained from phylogenetic tree. ITS sequences amplified from 11 specimens of two genera of ph. Basidiomycota: Marasmius and Ganoderma, were compared with ITS sequences from database using basic local alignment search tool (BLAST). Phylogenetic tree was constructed using Neighbor joining method based on differences between analyzed ITS sequences. Our results showed that within genera Marasmius and Ganoderma morphological and molecular determinations are usually in accordance, but for proper species delimitation both approaches should be used.

Key words: Basidiomycota, BLAST, Ganoderma internally transcribed spacer, species delimitation, Marasmius

Corresponding author: Eleonora Čapelja, University of Novi Sad, Faculty of Sciences, Department for Biology and Ecology, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia, +381 21 4852682, e-mail: eleonora.capelja@dbe.uns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp. 43 -48

Serbia

UDC 575:633.11
DOI: 10.2298/GENSR1401043G

Original scientific paper

GENETIC CONTROL AND HEREDITY OF HARVEST INDEX AND BIOLOGICAL YIELD IN BREAD WHEAT (Triticum aestivum L.)

Ahmad Reza GOLPARVAR

Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad UniversityIsfahan, Iran

Abstract

Golpavar Reza A. (2014): Genetic control and heredity of harvest index and biological yield in bread wheat (Triticum aestivum L.). Genetika, Vol 46, No. 1, 43-48.

Assessment of genetic control, mode of inheritance, general and specific combining abilities and effect of drought stress on genetic parameters of harvest index and biological yield traits in bread wheat were achieved by using Diallel mating design. Parents (eight cultivars) along with F₁ progenies (28 crosses) were sown in a randomized complete blocks design with three replications under stress condition in Karadj Agricultural Research Center. The data were analyzed according to methed of Hallauer and Miranda as well as fixed model of Griffing’s method II. Jinks-Hayman model was used to estimate broad and narrow-sense heritabilities and mean degree of dominance. There were significant differences between genotypes for mentioned traits in both environments. Studying mean of squares of general combining ability (GCA), specific combining ability (SCA), the ratio of GCA to SCA mean of squares and portion of additive and dominance variances showed importance of both additive and non additive gene effects for harvest index, but in biological yield heredity, additive effect was more important. Estimating broad-sense and narrow-sense heritabilities showed low efficiency of harvest index and high efficiency of biological yield for selection programs in stress environments.

Key words: biological yield, diallel mating design, harvest index, gene action, hexaploid wheat

Corresponding author: Ahmad Reza Golpavar, Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, P.O.Box:81595-158, Isfahan, Iran, e-mail:dragolparvar@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp.49 -58

Serbia

UDC 575.633.15
DOI: 10.2298/GENSR1401049F

Original scientific paper

DETERMINATION relevant breeding criteria by the path

AND factor analysis IN MAIZE

Milomir FILIPOVIĆ¹, Milosav BABIĆ¹, Nenad Delić¹, Goran Bekavac²,

Vojka BABIĆ^*1

¹Maize Research Institute, Zemun Polje, Belgrade, Serbia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Filipovic M., M. Babić, N. Delić, G.Bekavac and V. Babić (2014): Determination relevant breeding criteria by the path and factor analysis in maize-. Genetika, Vol 46, No. 1, 49-58.

In the process of plant breeding the application of relevant breeding criteria is very important. The Path analysis is broadly applied with the aim to define yield components that mostly determine the yield and that can be used as quality breeding criteria. However, the significance of revealed relationships between yield and yield components can be affected by various factors, such as diverse genetic material that is observed, traits included into analysis, environments in which the material is observed, as well as, the applied statistic approach to determine the nature of the relationships itself.

The interrelationships of yield and yield components of 15 commercial maize hybrids were observed using the Path and factor analyses. According to results of Path analysis, plant height, ear diameter and grain moisture had highly significant genetic and phenotypic direct effects on grain yield. At the same time, factor analysis points out significant effects of two factors on grain yield. Factor 1 was mostly determined by ear length and number of kernels per row, while grain moisture content, ear and cob diameter mostly determined Factor 2.

Key words: common structure, maize, Path coefficients, yield components

Corresponding author: Babić Vojka, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia; Tel: +38111 37 56 704; Fax: +38111 37 56 707; e-mail: vbabic@mrizp.rs

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Published in „GENETIKA“ Vol. 46, No.1 (2014), pp. 59 -73

Serbia

UDC 575:633.11
DOI: :10.2298/GENSR1401059P

Original scientific paper

EFFECT OF GROWING SEASON AND VARIETY ON QUALITY OF SPRING TWO-ROWED BARLEY

Novo PRŽULJ¹, Vojislava MOMČILOVIĆ¹, Jasmina SIMIĆ², Milan MIROSAVLJEVIĆ¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²MAIB-Mediterranean Agronomic Institute of Bari, Valenzano (Bari), Italy

Abstract

Pržulj N., V. Momčilović, J. Simić and M. Mirosavljević (2014): Effect of growing season and variety on quality of spring two-rowed barley. Genetika, Vol 46, No. 1, 59-73.

Quality of barley consists of precisely defined grain quality attributes (grain weight, grading, grain protein concentration, etc.) and malt quality attributes (extract, viscosity, Kolbach index, etc.). Barley quality attributes are quantitatively inherited and greatly affected by environmental factors such as temperature, available water, nitrogen fertilizer and soil type. The main objective of this study was to determine the effects of genotype and growing conditions (year) on grain and malt quality attributes in barley grown under field conditions in the Pannonian zone. The eight spring two-row barley varieties were studied during a seven growing seasons (1998-2004) on the location Novi Sad (45°20'N, 15°51'E, 86 m asl). The growing season predominantly affected variation of the all studied grain and malt quality attributes of spring barley where its percentage of variance was 35.2%, 20.2%, 32.5%, 25.4%, 30.9%, 31.2%, and 38.5% for grain weight (GW), grading (GRA), grain protein content (GPC), viscosity (VIS), Kolbach index (KOL), Hartong number (HAR) and extract content (EXT), respectively. The interaction of GxE comprised of 28.4%, 64.5%, 38.2%, 54.0%, 39.6%, 41.2% and 23.7% of variation for GW, GRA, GPC, VIS, KOL, HAR and EXT, respectively. The genetic component of variance ranged from 11.1% for GRA to 35.0% for EXT. The heritability was the lowest for GRA-0.54 and the highest for EXT-0.91. Across growing seasons GW ranged from 39.6 to 46.1g, GRA from 76.7 to 91.1%, GPC from 12.1 to 13.5g 100^-1g dm, VIS from 1.44 till 1.61m.Ps, KOL from 32.5 to 42,9%, HAR from 31.9 to 45.9VZ 45°C and EXT from 76.3 to 80.3% dm. Out of seven growing seasons EXT was acceptable in four ranging from79.2 to 81.4% dm. Although growing conditions in the Pannonian zone are less favorable for malting barley production in relation to western Europe it does not exclude malting barley production with acceptable quality in the Pannonian zone environments. The varieties Scarlett and Viktor performed quite acceptable quality in the Pannonian zone and may be recommended for commercial production in this region for malt industry.

Key words: Barley (Hordeum vulgare L), growing season, interaction, variety, quality, Pannonian zone

Corresponding author: Novo Pržulj, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, SerbiaTelefon: 021 4898 220, fax: 021 4898 222, e-mail: novo.przulj@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 46, No.1 (2014), pp. 75-94

Serbia

UDC 575
DOI: 10.2298/GENSR1401075J

Original scientific paper

EFFECTIVE CHEMICAL CONSTITUENT’S CONFIRMATION OF EXTRACTING SOLUTION FROM HERBS AND THEIR GENE TARGETS

Wen JIN^1,#, Qingqiong DENG²^,#, Meiying NIU^1,#, Liping HU¹, Aijuan LIU¹, Guihua TIAN^3,*, Jianqiu LU^1,*, Mingquan ZHOU², Ghulam MURTAZA*⁴, Haiyan JIANG²

¹ Beijing University of Chinese Medicine, ChaoYang District, Beijing, P. R. China.

²Beijing Normal University, HaiDian District, Beijing, P. R. China.

³Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, P. R. China

⁴Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Pakistan

Abstract

Jin W., Q. Deng, M. Niu, L Hu, A. Liu, G.Tian, J.Lu, M. Zhou, G. Murtaza and H. Jiang (2014): Effective chemical constituent’s confirmation of extracting solution from herbs. Genetika, Vol 46, No. 1, 75-94.

Ixeris sonchifolia Hance, a normal perennial herb, has effects on appendicitis, headache, menstruation, blood circulation, blood stasis, pain, fibrinolytic enzymes, thrombus formation, coronary artery, myocardial infarct size, PGI2, ET, TXA2, TNF-,αIL-6 and IL-8, etc . However, there was no full coverage about the molecular basis and gene targets of Ixeris sonchifolia injection. PubChem Database and DAVID Database had been used conjunctively to detect the gene IDs, gene targets and their functions. There were 3008 genes found in the final results, 16 kinds of definite functions had been detected, 9 of which had been proved and the effects were focused on the psychological, immune, neurological, metabolic, cardiovascular and cancer functions. Ixeris sonchifolia had a wide effects on various symptoms, which had been reported by previous experiments and verified by this research; however its influences on aging, chemdependency, developmental, normalvariation, reproduction, pharmacogenomic and psychologic functions should be confirmed in the future experiments.

Key words: database, gene targets, functions, information integration, Ixeris sonchifolia Hance

Corresponding author: Ghulam Murtaza, E-mail: gmdogar356@gamil.com; Phone: +92-992-383591-5; Fax: +92-992383441

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Published in „GENETIKA“ Vol. 46, No. 1 (2014), pp. 95 - 104

Serbia

UDC 575

DOI: 10.2298/GENSR1401095P

Original scientific paper

THE ASSESSMENT OF GENETIC POTENTIAL IN PERFORMANCE TESTED GILTS BY MEANS OF SELECTION INDEXES METHOD

Mladen POPOVAC¹*, Milica PETROVIĆ¹, Dragan RADOJKOVIĆ¹, Dragan STANOJEVIĆ¹, Aleksandar MILETIĆ², Predrag PERIŠIĆ¹

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

² PKB Agroekonomik Institute, Belgrade, Serbia

Abstract

Popovac M., M. Petrović, D. Radojković, D. Stanojević, A. Miletić, and P. Perišić (2014): The assessment of genetic potential in performance tested gilts by means of selection indexes method-. Genetika, Vol 46, No. 1, 95-104.

The objective of this research paper was to make an assessment of breeding value of performance tested gilts of Swedish Landrace and F₁ cross-breds of Swedish Landrace and Great Yorkshire by the method of selection index. The traits on whose basis the breeding value was estimated were: daily liveweight gain, average backfat thickness measured at two sites and carcass meat percentage. These traits were corrected for body mass of 100kg by the method of base indexes and the following average values were determined: corrected daily liveweight gain (KZDP) 408.93g/day, corrected average backfat thickness measured at two sites (KSL) 9.77mm and corrected carcass meat percentage (KPM) 61.08%. Studying the effect of genotype, year and birth season of gilts a statistically significant variation (P>0.05) of these traits provoked by the mentioned factors was not determined while the gilts` sire statistically highly significantly (P<0.001) influenced all studied traits. Heritability coefficients were: h²= 0.255 for KZDP, h²= 0.356 for KSL and h²= 0.349 for KPM. The four selection index equations were constructed among which as the most optimal was chosen the one which includes all three traits (KZDP, KSL and KPM) and whose coefficient of the correlation of selection index and aggregate genotype was r_IAG = 0.594.

Key words: backfat thickness, liveweight daily gain, meat percentage, heritability, selection index

Corresponding author: Popovac Mladen, University of Belgrade, Faculty of Agriculture Belgrade, Nemanjina 6, Zemun, Serbia; mlp@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 105 - 115

Serbia

UDC 575:633.11

DOI: 10.2298/GENSR1401105I

Original scientific paper

Khorasan WHEAT POPULATION RESEARCHING (Triticum turgidum, SSP. TURANICUM (McKEY) IN THE MINIMUM TILLAGE CONDITIONS

Jela Ikanović¹, Vera Popović², Snežana Janković³, Ljubiša Živanović¹,

Sveto Rakić¹, Dalibor Dončić⁴

¹University in Belgrade, Faculty of Agriculture, Belgrade-Zemun, Serbia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Institute of applyed research in Agriculture, Belgrade, Serbia

⁴Syngenta-Agro, Gradiska, Bosnia and Herzegovina

Abstract

Ikanović J., V. Popović, S. Janković, Lj. Živanović, S.Rakić and D. Dončić (2014): Khorasan wheat population researching (Triticum turgidum, ssp. turanicum (McKEY) in the minimum tillage conditions .- Genetika, Vol 46, No. 1, 105 -115.

Khorasan wheat occupies a special place in the group of new-old cereals (Triticum turgidum, ssp. Turanicum McKey). It is an ancient species, native to eastern Persia, that is very close to durum wheat by morphological characteristics. Investigations were carried out in agro ecological conditions of the eastern Srem, with two wheat populations with dark and bright awns as objects of study. The following morphological and productive characteristics were investigated: plant height (PH), spike length (SH), number of spikelets per spike (NSS), absolute weight (AW) and grain weight per spike (GW), seed germination (G) and grains yield (YG). Field micro-experiments were set on the carbonate chernozem soil type on loess plateau in 2011 and 2012. Hand wheat sowing was conducted in early March with drill row spacing of 12 cm. The experiment was established as complete randomized block system with four replications. Tending crops measures were not applied during the growing season. Plants were grown without usage of NPK mineral nutrients. Chemical crop protection measures were not applied, although powdery mildew (Erysiphe graminis) was appeared before plants spike formation in a small extent.

The results showed that both populations have a genetic yield potential. In general, both populations manifested a satisfactory tolerance on lodging and there was no seed dispersal. Plants from bright awns population were higher, had longer spikes and larger number of spikelet’s per spike. However, plants from dark awns population had higher absolute weight and grains weight per spike, as well as grain yield per plant. Strong correlation connections were identified among the investigated characteristics. The determination of correlations, as well as direct and indirect affects, enabled easier understanding of the mutual relationships and their balancing in order to improve the yield per unit area.

Key words: Triticum turgidum, population with dark and bright awns , genetic potential, morphological and productive characteristics.

Corresponding author: Jela Ikanovic, Faculty of Agriculture, Belgrade,Tel: +381 11 2615 315; E-mail: jela@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.1(2014), pp. 117 -128

Serbia

UDC 575:630
DOI: 10.2298/GENSR1401117P

Original scientific paper

ANALYSIS OF INTER-LINE VARIABILITY OF BALD CYPRESS (Taxodium distichum L. Rich.) JUVENILE SEEDLINGS using morphometric markers

Vladan POPOVIĆ1, Aleksandar LUČIĆ¹, Mirjana ŠIJAČIĆ-NIKOLIĆ2, Tatjana ĆIRKOVIĆ-MITROVIĆ¹, Ljubinko RAKONJAC1, Branislav CVJETKOVIĆ3, Snežana MLADENOVIĆ DRINIĆ4

¹Institute of Forestry, Belgrade

²Faculty of Forestry, Belgrade

³Faculty of Forestry, Banja Luka

⁴Maize Research Institute, Zemun Polje, Belgrade

Abstract

Popović V., A. Lučić, M. Šijačić-Nikolić, T. Ćirković-Mitrović, Lj. Rakonjac, B. Cvetković and S. Mladenović Drinić (2014): Analysis of inter-line variability of bald cypress (Taxodium distichum L. Rich.) juvenile seedlings using morphometric markers-. Genetika, Vol 46, No. 1, 117-128.

In this paper are presented results of the analysis of inter-line variability of morphometric characters of Bald cypress juvenile seedlings at the level of 20 half-sib lines. Obtained results contribute to better understanding of relationship of analyzed characters, as well as the influence of analyzed characters to the differentiation of half-sib lines. Based on these results, differences were observed in the values of the analyzed characters for each half-sib line individually. The determined values of seedlings’ size indicate good genetic and adaptive potential of this species, which can be considered as a starting point for the mass production of quality planting material and its use in Serbia.

Key words: Bald cypress, genetic potential, seedlings, variability.

Corresponding author: Vladan Popović; Institute of Forestry; Kneza Višeslava 3; 11000 Belgrade; Serbia; Phone: 011-3553355; e-mail: vladanpop79@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 129-136

Serbia

UDC 575.634
DOI: 10.2298/GENSR1401129Y

Original scientific paper

THE MAIN QUALITY ATTRIBUTES OF NON-SPRAYED CHERRY LAUREL (Laurocerasus officinalis Roem.) GENOTYPES

Hilal YILDIZ¹, Sezai ERCISLI², Haluk Kemal NARMANLIOGLU³, Saban GUCLU⁴,

Mustafa AKBULUT⁵ and Zumrut TURKOGLU⁶

¹Gumushane University, Engineering Faculty, Department of Food Engineering, Gumushane-Turkey

²Ataturk University Agricultural Faculty Department of Horticulture Erzurum-Turkey

³Ataturk University, Hamza Polat Vocational School, Ispir, Erzurum -Turkey

⁴Bozok University, Agricultural and Natural Science Faculty, Erdogan Akdag Kampusu, Yozgat-Turkey

⁵Recep Tayyip Erdogan University, Agricultural and Natural Science Faculty, Zafer Mah. Katori Mevkii, Pazar Yerleskesi, Pazar, Rize-Turkey

⁶Ministry of Food, Agriculture and Livestock, Ataturk Orman Ciftligi, Ankara-Turkey

Abstract

Yildiz H., S. Ercisli, H.K. Narmanlioglu, S.Guclu, M. Akbulut and Z. Turkoglu (2014): The main quality attributes of non-sprayed cherry laurel (Laurocerasus officinalis Roem.) genotypes. Genetika, Vol 46, No. 1, 129-136.

Cherry laurel (Laurocerasus officinalis) is naturally growing in black sea region in Turkey and the trees has not been spraying with pesticides. In natural growing conditions, all cherry laurel genotypes particularly fruits are seems resistant against pests. Astringent nature of fruits forms a barrier for pests. A total twelve cherry laurel accessions were harvested at full maturation time from various sites in Of district located in eastern black sea region in Turkey. Fruits were analyzed for their total phenolic (TP), total monomeric anthocyanin (TMA), total carotenoid (TC), Vitamin C and for their antioxidant capacity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) in fruit flesh extract. Variability among accessions was greatest for total phenol (TP) and total anthocyanins content ranged from 154 to 213 mg GAE per 100 g and 397 to 519 mg per 100 g. The results indicated that cherry laurel fruits are superior and unique in terms of bioactive content in particular compared to bioactive rich fruit species.

Key words: Cherry laurel, Genetic Resources, Total phenolics, Genotype, Total anthocyanin

Corresponding author: Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: 090 4422312599, Fax: 090 4422360958, e-mail: sercisli@gmail.com

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Published in „GENETIKA“ Vol. 46, No.1(2014), pp. 137 - 148

Serbia

UDC 575:633
DOI: 10.2298/GENSR1401137J

Original scientific paper

VIABILITY OF OILSEED RAPE (Brassica napus L.) SEEDS

UNDER SALT STRESS

Dušica JOVIČIĆ, Zorica NIKOLIĆ, Miroslav ZORIĆ, Ana MARJANOVIĆ-JEROMELA, Gordana PETROVIĆ, Dragana MILOŠEVIĆ, Maja IGNJATOV

Intitute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Jovičić D., Z. Nikolić, M. Zorić, A. Marjanović-Jeromela, G. Petrović, D. Milošević and M. Ignjatov (2014): Viability of oilseed rape (Brassica napus L.) seeds under salt stress-. Genetika vol 46, No1, 137-148.

Although salt stress affects all plant growth stages, seed germination and seedling growth stages are the most sensitive. Therefore, the aim of this study was to examine the seed viability of selected oilseed rape cultivars in saline conditions and to determine the most tolerant cultivars to salinity stress at germination stage. The experiment included four released oilseed rape cultivars. The salinity stress was simulated in vitro conditions by adding different concentrations of NaCl solution to the growing media. Testing of the seed viability was performed by using the standard laboratory method test and the accelerated aging test. In all four cultivars, with increasing concentrations of NaCl in the growing medium, significant changes were observed in determined characteristics. Results of accelerated aging test were lower than the standard laboratory test, which implies the importance of vigour tests as an additional indicator of physiological seed quality, especially under stress conditions. The most important source of variation is treatment, but the differences between the cultivars were also significant. Depending on traits, crossover and non-crossover cultivar × treatment interactions were observed.

Key words: accelerating aging test, germination, growth variables, salinity tolerance, standard laboratory method

Corresponding author: Dušica Jovičić,Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia,Phone: 021/4898-154, Fax: 021/421-249,e-mail:dusica.jovicic@nsseme.com

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 149- 158

Serbia

UDC 575

DOI: 10.2298/GENSR1401149K

Original scientific paper

THE INFLUENCE OF CAST/MspI, HinfI, RsaI POLYMORPHISM ON PRODUCTION TRAITS IN PIGS

Eva Kluzakova, Roman Stupka, Michal Sprysl, Jaroslav Citek,

Monika Okrouhla

Czech University of Life Science Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic.

Abstract

Kluzakova E., R. Stupka, M. Sprysl, J. Citek and M. Okrouhla (2014): The influence of CAST/MspI, HinfI, RsaI polymorphism on production traits in pigs. Genetika, Vol 46, No. 1, 149-158.

The aim of this study was to perform the CAST gene polymorphism genotyping and to verify its possible influence on the quantitative and qualitative indicators characterizing carcass value in pigs. The study found a significant effect of the CAST gene on carcass value. In the case of alelle A present in the CAST/HinfI gene there was a higher lean meat share (i.e. lower fat content) and therefore the detected quality of pork meat was lower. The signifiant differences were found between the homozygotes AA and heterozygotes AB, mainly in the amount of fat content (P≤0.05). Concerning the CAST/MspI gene, it was found that genotype CD caused higher lean meat share due to the higher shares of muscles in the main meat parts. However higher lean meat share does lead to lower quality of the meat. Another discovered influence was that of the allele D, which was associated with the intramuscular fat content (IMF) in the neck (P≤0.05). Our results also show signifiant influence (P≤0.05) of the allele C on the qualitative indicators of pork meat (MS EC₅₀). Concerning the CAST/RsaI polymorphism, the study proved that this polymorphism doesn’t influence any of the monitored qualitative parameters.

Key words: allele, CAST, pig, polymorphism, qualitative traits

Corresponding author: Eva Kluzakova, Czech University of Life Science Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic,e.mail: kluzakova@af.czu.cz

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 159- 168

Serbia

UDC 575.633
DOI: 10.2298/GENSR1401159H

Original scientific paper

Mode of inheritance and Combining ability for plant height

and head diameter in sunflower (Helianthus annuus L.)

Nada Hladni¹, Vladimir Miklič¹, Siniša Jocić¹, Marija Kraljević-Balalić²,

Dragan Škorić³

¹ Institute of Field and Vegetable Crops, Novi Sad, Serbia

² Faculty of Agriculture, Novi Sad, Serbia

³Serbian Academy of Science and Arts, Branch in Novi Sad, Serbia

Abstract

Hladni N., V. Miklič, S. Jocić, M. Kraljević-Balalić, D. Škorić (2014): Mode of inheritance and combining ability for plant height and head diameter in sunflower (Helianthus annuus L.).-Genetika, Vol 46, No. 1, 159-168.

The development of sunflower hybrids with high genetic potential for seed and oil yields requires information on the general combining ability (GCA) and specific combining ability (SCA) for plant height and head diameter in the F₁ generation. Used in this study were thirteen new divergent cms inbred lines (A), three Rf restorers utilized as testers, and their F₁ hybrids. The inbred A-lines, Rf-testers and F₁ hybrids differed significantly in the mean values for plant height and head diameter. The mode of inheritance for plant height was superdominance of the better parent and for head diameter it was dominance and superdominance of the better parent. Highly significant positive values of GCA for both traits were found in cms inbred line NS-G-7 and restorer line RHA-N-49. Based on the GCA values chosen were lines with the best GCA for plant height (NS-G-9, NS-G-7) originating from PRA-RUN and head diameter (NS-G-13, NS-G-12) originating from DES. The greatest highly significant positive SCA value was found in NS-G-1xRHA-N-49 for plant height and in NS-G-8xRUS-RF-ol-168 for head diameter. Non-additive genetic variance played the main role in the inheritance of both traits as confirmed by the GCA/SCA ratios in the F₁generation, which were invariably smaller than unit. The female A lines had the highest contribution to the expression of head diameter and plant height.

Key words: combining ability, gene effect, head diameter, plant height, sunflower

Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, fax: +381 21 6413833, phone: 021 4898411, e-mail: nada.hladni@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 169-178

Serbia

UDC 575:633.11
DOI: 10.2298/GENSR1401169K

Original scientific paper

Different genotypes of alternative small grains

in organic farming

Dušan Dj. KOVAČEVIĆ^*1, Svetlana ROLJEVIĆ², Željko DOLIJANOVIĆ¹,

Snežana DJORDJEVIĆ¹, Vesna MILIĆ³

¹University of Belgrade, Faculty of Agriculture, Belgrade, R. Serbia

²Institute of Agricultural Economics, Belgrade, R. Serbia

³University of East Sarajevo, Faculty of Agriculture, East Sarajevo, R. Bosnia and Herzegovina

Abstract

Kovacevic D., S. Roljević, Ž. Dolijanović, S. Djordjević, and V. Milić (2014): Different genotypes of alternative small grains in organic farming. -Genetika, Vol 46, No. 1, 169-178.

The main objectives were to examine different genotypes of alternative small grains important for food technology in organic farming conditions on morphological and productive characteristics. Four genotypes of different alternative small grains were included in the trial. Three of them were chosen for specific usage in food technology compact wheat Bambi - Triticum aestivum L. ssp. compactum, spelt Nirvana (Triticum aestivum L. ssp. spelta), durum wheat Durumko- (Triticum durum L.), and one which leads as a genotype for intensive conventional common wheat production in Serbia - NS 40S (Triticum aestivum L. ssp. vulgare).

Plots were fertilized with biohumus "Royal ofert" (30 t ha^-1) applied in autumn with basic tillage and microbial fertilizer "Slavol" ad as in spring foliar treatment in full tillering (5 l ha^-1).

Alternative small grains durum wheat and compact wheat except splet gives lower grain yield in organic condition compared with comercial cultivar for high-input NS-40S.

Key words: alternative small grains, genotypes, organic farming, yield

Corresponding author: Dušan Dj. Kovačević, University of Belgrade, Faculty of Agriculture, Institute of Field and Vegetable crops, dulekov@agrif.bg.ac.rs; +381 11 2615-315 ext. 356, fax: +381 11 3161-987

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 179-185

Serbia

UDC 575:633
DOI: 10.2298/GENSR1401179M

Original scientific paper

EVALUATION OF GENETIC VARIANCE COMPONENTS FOR SOME QUANTITATIVE TRAITS IN RAPESEED (Brassica napus L.)

Ana MARJANOVIĆ JEROMELA¹, Radovan MARINKOVIĆ¹,

Milan JOCKOVIĆ¹, Petar MITROVIĆ¹, Željko MILOVAC¹, Nikola HRISTOV¹,

Jasna SAVIĆ²,Biljana STAMENKOVIĆ³

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

³Faculty for biofarming, Bačka Topola, Serbia

Abstract

Marjanović-Jeromela A., R. Marinković, M. Jocković, P. Mitrović, Ž. Milovac, N. Hristov, J. Savić and B. Stamenković (2014): Evaluation of genetic variance components for some quantitative traits in rapeseed (Brassica napus L.).Genetika, Vol 46, No. 1,179 -185.

Three hybrid combinations obtained by crossing six winter rapeseed cultivars were analyzed for the impact of genes with additive and dominant effects and their interactions with inheritance of plant height and first lateral branch height. The linkage among the expected progeny means was checked using the scaling test method (Mather, 1949), while the estimates of genetic effects and mode of inheritance was made by the Generation Mean Analysis (Mather and Jinks, 1982). The additive dominant model did not prove adequate for plant height in all three crosses, and for first lateral branch height in the second and third cross. The inadequacy of the model showed epistatic gene effects were also of large importance in the inheritance of these traits.

Duplicate epistasis for plant height inheritance was found in all three cross combinations and for inheritance of height of the first lateral branch in second and third cross combination. However, it should be emphasized that duplicate epistasis among dominant positive genes occurred on plant height inheritance in C1 and C3, and on inheritance of first lateral branch height in C3. Duplicate epistasis among dominant negative genes occurred in C2 on the mode of inheritance of both traits.

Key words: additive and dominant effect, epistasis, height of the first lateral branch, plant height

Corresponding author: Ana Marjanović Jeromela, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 187-198

Serbia

UDC 575
DOI: 10.2298/GENSR1401187A

Original scientific paper

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS

OF DIFFERENT RICE CULTIVARS BY MICROSATELLITE MARKERS

Mehrzad ALLHGHOLIPOUR^1*, Ezatollah FARSHDFAR², Babak RABIEI³

1* Department of Plant Breeding, Rice Research Institute of Iran (RRII), PRasht/Iran

2 Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, Razi University, Kermanshah, Iran

3 Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Allhgolipour M., E. Farshdfar, and B. Rabiei (2014): Molecular characterization and genetic diversity analysis of different rice cultivars by microsatellite markers-. Genetika, Vol 46, No. 1, 187-198.

A total of 52 rice SSR markers well distributed on 12 chromosomes were used to characterize and assess the genetic diversity among ninety four rice genotypes. The total number of polymorphic alleles was 361 alleles with the average of 5.86 alleles per SSR locus. The study revealed that some markers such as RM276 and RM5642 on chromosome 6 and RM14 and RM1 on chromosome 1 have more than 9 observed alleles compared to other primers like RM16, RM207, RM208 and RM317 with 3-4 alleles. The highest and lowest PIC values were observed for primers RM276 (0.892 and RM208 (0.423) respectively. Using Shannon´s diversity index, a mean genetic diversity of 1.641 was obtained from the analysis, indicating a high level of genetic variation among these cultivars. Cluster analysis using the complete linkage method based on jaccard similarity coefficient revealed that all genotypes were classified to nine clusters at genetic similarity level of 0.01-0.75, which contained 12, 16, 2, 18, 3, 6, 16, 10 and 11 varieties, respectively. Results of discriminant analysis showed that the nine cluster groups were confirmed at high levels of correct percent (96.8) and revealed true differences among these clusters. As a final result from this study, we selected eight cultivars from different cluster including Daylamani, Tarom mohali (landrace rice cultivars), RI1843046, Back cross line, RI184472, RI184421 (promising cultivars), Line 23 and IR50 (IRRI lines) as parents. All of the selected cultivars will be arranged in complete diallel design to obtain combining abilities, gene effects and heterosis for each important morphology and physico-chemical characters.

Key words: Cluster analysis, genetic diversity, rice cultivars, SSR marker

Corresponding author: Mehrzad Allhgholipour,^,Department of Plant Breeding, Rice Research Institute of Iran (RRII), P.O Box: 1658, Postal Code: 41996-13475/ Rasht/Iran

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 199-207.

Serbia

UDC 575:630
DOI: 10.2298/GENSR1401199M

Original scientific paper

Genetic diversity in a collection of ornamental squash (Cucurbita pepo L.)

Emina Mladenović¹^*, Janoš Berenji², Vladislav Ognjanov¹,

Mirjana Ljubojević¹, Jelena Čukanović¹and Tihomir salamun¹

¹University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

²Institute for field and crops, Novi Sad, Serbia

Abstract

Mladenović E., J. Berenji, V. Ognjanov, M. Ljubojević, J. Čukanović and T. Salamun (2014): Genetic diversity in a collection of ornamental squash (Cucurbita pepo L.) - Genetika, vol., No.46, No.1, 199-207.

The aim of this study is collecting and preserving of unique Cucurbita pepo germplasm in Serbia. This study also describes the intraspecific variation of C. pepo within twenty germplasm accessions. Conservation work aimed to develop a representative core collection of C. pepo germplasm, to guide future studies and breeding of its ornamental use. A wide range of C. pepo germplasm was collected from diferent parts of the world. Nineteen variables were recorded in 20 varieties to determine the overall degree of polymorphism and to detect similarities among them. Qualitative traits of fruit such is shape, color and texture, showed immense variation. Coefficient of variation were highest for fruit length, fruit weight and number of fruits per plant (CV=56.69-161.32%), while they were the lowest for leaf length (CV=20.65%). Morphological characterization is need to facilitate the use of C. pepo varieties in breeding work.

Based on the PCA results, 20 accessions of squash are separate in five groups. Those groups are unique in qualitative and quantitative traits. Knowledge of genetic divergence among varieties is essential for breeding.

Key words: Cucurbita pepo, genetic resources, PCA, variability

Corresponding author: Emina Mladenović, University of Novi Sad, Faculty of Agriculture, Department of fruit science, viticulture, horticulture and landscape arhitecture, Dositeja Obradovića Square 30, 21000 Novi Sad, tel: +38121 485 3269, fax:+381 21 450 123, e-mail: eminam@polj.uns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 209-218

Serbia

UDC 575
DOI: 10.2298/GENSR1401209K

Original scientific paper

MtDNA VARIATION WITHIN LOCAL HUMAN POPULATIONS IN BOSNIA

AND HERZEGOVINA

*Lejla KAPUR-POJSKIĆ, *Naris POJSKIĆ, Gabrijela RADOSAVLJEVIĆ,

Damir MARJANOVIĆ, Rifat HADŽISELIMOVIĆ

Institute for Genetic Engineering and Biotechnology, University of Sarajevo

Abstract

Kapur-Pojskić L., N. Pojskić, G. Radosavljević, D.Marijanović and R. Hadžiselimović (2014): MtDNA variation within local human populations in Bosnia and Herzegovina.- Genetika, Vol 46, No. 1, 209-218.

Being a crossroad of many ancient and recent historical migrations, Bosnia and Herzegovina (B&H) represents unique spot of multicultural and social diversity. The main aim of this study was to assess genetic structure of three local populations of mountain area from central part of B&H using mtDNA HVS-1 as an informative marker for population genetics studies.

A 444 bp HVS-1 segment of control region of mtDNA extracted from buccal swabs was PCR amplified and sequenced. Haplotype and nucleotide diversity, average number of nucleotide differences, AMOVA and pairwise F_ST based on mtDNA haplotype and haplogroup frequencies were calculated. NJ tree was constructed based on pairwise F_ST results. Tajima’s D was calculated to evaluate population demographic status.

Key words: human mtDNA diversity, HVS1 motif, haplogroups, population structure

Corresponding author: Naris Pojskic, Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina, Phone:+38733220926, fax:+38733442891, E mail: naris.pojskic@ingeb.ba

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 219-226

Serbia

UDC 575
DOI: 10.2298/GENSR1401219P

Original scientific paper

POPULATION-GENETICAL CHARACTERISTICS OF THE BEE POPULATION

OF VOJVODINA

Ivan Pihler¹, Hrisula Kiprijanovska², Nada Plavša¹, Milan Krajinović¹, Aleksandar Uzunov², Per KRYGER³, Nebojša Nedić⁴

¹University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia.

²University of Skopje, Faculty of Agricultural Science and Food, Skopje, Macedonia.

³University of Arhus, Department of Integrated Pest Management, Slagelse, Denmark

⁴University of Belgrade, Faculty of Agriculture, Belgrade, Serbia.

Abstract

Pihler I, H. Kiprijanovska, N. Plavša, M. Krajinović, A.Uzunov. P.Kryger and N. Nedić (2014): Population-genetical characteristics of the bee population of Vojvodina . Genetika, Vol 46, No. 1, 219-226.

In 1893. Jovan Živanović notes that based on the color of the abdomen of bees, two varieties of bees are present in Vojvodina, one dark and the other with two yellow rings on abdomen. Up to the present time there are mixed opinions about the presence of one or more populations of bees in Vojvodina, especially about the presence of the yellow variety in Banat. The aim of this paper is to establish the existence of one or more populations of bees, using genetic analysis of bees. Evaluation of genetic connections, diversity within the population and structure of the bee population in Vojvodina, were calculated on the basis of allele variation of 25 microsatellite loci. A genetic typification of the following microsatellites was performed: A8, A14, A24, A29, A43, A79, A88, A113, Ac11, Ac88, Ac139, Ac306, Ap15, Ap68, Ap85, Ap90, Ap223, Ap224, Ap226, Ap249, Ap273, Ap274, Ap288, At168, At188. 92% or 23 loci proved to be polymorphic in samples of bees from Srem and Bačka, and 88% or 22 loci proved to be polymorphic in samples of bees from Banat. Heterozygosity calculated for the whole population is not significantly different from the expected heterozygosity. It was found that the obtained genetic differences between bees of Srem and Bačka, and Banat region are not sufficient for these two populations to be considered separate.

Key words: A. m. carnica, alleles, bee, genetic analysis

Corresponding author: Pihler Ivan, Trg Dositeja Obradovića 8., 21000 Novi Sad, tel: 0641352899, email: ivan.pihler@stocarstvo.edu.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 227-234

Serbia

UDC 575
DOI: 10.2298/GENSR1401227K

Original scientific paper

COMPLETE ABSENCE OF LINKAGE DISEQUILIBRIUM BETWEEN ENZYME LOCI IN NATURAL POPULATIONS OF Drosophila ananassae

SANJAY KUMAR and A. K. SINGH*

Department of Zoology, Banaras Hindu University, Varanasi, INDIA

Abstract

Kumar S., and A.K.Singh (2014): Complete absence of linkage disequilibrium between enzyme loci in natural populations of Drosophila ananassae.Genetika, Vol 46, No. 1, 227--234.

Linkage disequilibrium has been studied among three linked enzyme loci of second chromosome of D. ananassae collected from five natural populations. Each of the three enzyme loci, that is Acph1, Acph 2 and Xdh was represented by two distinct alleles and in three genotypic forms. Thus nine genotypic combinations were recorded for each enzyme pair. The results clearly show absence of non-random occurrence of different genotypic combinations in all the populations studied. The occurrence of all possible genotypic combinations indicates enough frequency of crossing over among these enzyme loci. The absence of linkage disequilibrium in this study thus indicates that selection did not play any role and free recombination among the genes resulted random occurrence of all combinations.

Key words: Allozyme, crossing-over, D. ananassae, linkage disequilibrium

Corresponding author: A. K. Singh, Department of Zoology, Banaras Hindu University, Varanasi-221005 India, e-mail: aksbhu23@rediffmail.com

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 235-242

Serbia

UDC 575
DOI: 10.2298/GENSR1401235S

Original scientific paper

MOLECULAR CHARACTERISATION OF SOME LIGNICOLOUS SPECIES FROM FUNGAL CULTURE COLLECTION

Nevena STEVIĆ¹, Eleonora ČAPELJA¹, Vladislava GALOVIĆ ², Milana NOVAKOVIĆ¹,

Maja KARAMAN¹

¹University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia

² Institute of Lowland F

Abstract

Stević N., E. Čapelja, V. Galović, M. Novaković, and M. Karaman (2014): Molecular characterisation of some lignicolous species from fungal culture collection-. Genetika, Vol 46, No. 1, 235-242.

Culture collections of microorganisms, including fungi, are strain deposits recognised as Biological Resource Centers (BRCs) with a great importance in science, industry and education. Their objective is to preserve the purity, viability and genomic integrity of every single strain as a member of such collection. Since improvement of molecular methods nowadays brought many novel approaches in manipulation with strains of microorganisms, they can also be useful for characterization of existing stored strains.

ITS1 region in nuclear DNA is preferred barcoding marker for taxon identification, which can be explained by its great inter-species variability. This paper presents results from analysing ITS1 region sequences (17) obtained from fungal DNA of culture collection of autochthonous, lignicolous genera Piptoporus, Pleurotus, Ganoderma and Schizophyllum cultured on malt agar plates for 14 days at 25°C. BLAST (Basic Local Alignment Search Tool) was used for comparison with online databases, while alignment of sequences was made with MEGA 5.10 software. Morphological determination of species or genus was confirmed for 13 cultures, while the others were disproved. The resulting alignment indicated small intra-species variability of ITS1 region and pointed to it as an ideal marker for verification of fungal culture collections' authenticity.

Key words: BLAST, culture collection, fungi, ITS1 region, molecular identification.

Corresponding author: Stević R. Nevena, University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia, e-mail: nevena.stevic@dbe.uns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 243-253

UDC 575:633.15
DOI: 10.2298/GENSR1401243J

Original scientific paper

IMPACTS OF IRRIGATION AND GENOTYPE ON YIELD, PROTEIN, STARCH

AND OIL CONTENTS IN GRAIN OF MAIZE INBRED LINES

Marko JOSIPOVIC¹, Hrvoje PLAVSIC¹, Vlado KOVACEVIC²,

Monika MARKOVIC²and Dario ILJKIC²

¹ Agricultural Institute, Osijek, Croatia

²University J.J. Strossmayer in Osijek, Faculty of Agriculture, Osijek, Croatia

Abstract

Josipovic M., H. Plavsic, V. Kovacevic, M. Markovic, and D. Iljkic (2014): Impacts of irrigation and genotype on yield, protein, starch and oil contents in grain of maize inbred lines. Genetika, Vol 46, No. 1,243 -253.

Four inbred lines of maize (Os 438-95 = C1, Os 30-8 = C2, Os 6 = C3 and Os 1-44 =C4) were grown for 4-year period (2006-2009) in the stationary field experiment on Osijek eutric cambisol. Impact of irrigation, nitrogen fertilization and genotype were tested. Soil moisture was maintained by two irrigation rates from 60-100% and 80-100% of the field water capacity). Two steps of N (0, 100 and 200 kg N ha^-1) were applied, while P and K fertilization was equal (500 kg/ha NPK 0:30:20). Eight maize genotypes (four inbred lines and four hybrids) were grown on each basic plot of fertilization. The experiment was duplicated for maize – soybean rotation. The experiment was set by split-split plot method according to randomized block design in three replicates. The basic plot areas were 617.2 m² (irrigation), 313.6 m² (fertilization) and 39.2 m²(genotype). Selection of N non-fertilized treatment and four inbred lines were made for this study with aim of testing year (A) irrigation (B) and genotype (C) effects under natural N-soil conditions. Average grain yield in level 1809 kg ha^-1without N fertilization is indication of very high fertility of the soil. Differences of yield among the years were from 823 (2007) to 2450 (2006) kg ha^-1. Excessive drought and high air-temperature stress is responsible for the low maize yield in 2007. Irrigation considerable affected on maize yields (4-year averages: 1500, 1809 and 2118 kg ha^-1, for B1, B2 and B3, respectively). Differences of the 4-year average yields among the genotypes were from 1259 (C3) to 2765 (C1) kg ha^-1. Differences of yield among the genotypes in the different years were also considerable because the lowest yield was for 71% (A1), 23% (A2), 63% (A3) and 40% (A4) lower in comparison to the highest yield. The genotype effects under different water supplies were less influencing factor because the high-yielding C1 had for 128%, 129% and 106% the higher yield compared to the low-yielding C3, for B1, B2 and B3, respectively. Differences of grain -protein, -starch and -oil among the years was from 9.61 to 11.84%, from 68.51% to 70.93% and from 3.50% to 4.17%, respectively. The C2 separated by the higher grain protein contents (10.93%) from the remaining three genotypes (average 9.96%). The genotype effects on starch and oil contents were from 69.83% (C4) to 70.58% (C1) and from 3.56% (C3) to 4.09% (C1), respectively.

Key words: inbred lines, irrigation, yield, maize, protein, oil, starch

Corresponding author: Vlado Kovačević, Faculty of Agriculture, Kralja P. Svačića 1d,

31000 Osijek, Croatia; phone: +385 31 554 932, fax: +385 31 554 853; e-mail: vkovacevic@pfos.hr

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 255-268

Serbia

UDC 575
DOI: 10.2298/GENSR1401255K

Original scientific paper

GRADUAL LOSS OF GENETIC DIVERSITY OF Ambrosia artemisiifolia L. POPULATIONS IN THE INVADED RANGE OF CENTRAL SERBIA

Nataša KOČIŠ TUBIĆ, Mihajla DJAN, Nevena VELIČKOVIĆ, Goran ANAČKOV,

Dragana OBREHT

Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad

Abstract

Kocić Tubič N., M. Djan, N. Veličković, G. Anačkov and D. Obreht (2014): Gradual loss of genetic diversity of Ambrosia artemisiifolia L. populations in the invaded range of central Serbia.-Genetika, Vol 46, No.1,255 -268.

As an invasive allergenic weed, Ambrosia artemisiifolia L. causes serious public health and economic problems in invaded ranges of Europe. Over the last two decades, while expanding toward southern parts of Serbia, this common ragweed has become a very troublesome plant species in the whole country. Considering the importance of genetic studies in understanding of invasive species, our main objectives in this study were to analyze the genetic diversity and genetic structure of Ambrosia artemisiifolia populations from Central Serbia, a relatively recently invaded region. Comparing values of genetic measures obtained by microsatellite analyses, a number of differences were detected in genetic diversity between sampled populations. Allelic richness-r (ranged from 5.42 to 7.80), the mean number of alleles per locus-N_A (5.8-8.4) and the mean number of rare alleles per locus-N_R (2.8-5.8) have quite similar ranges across populations. We observed greater genetic variability in populations from the northern part of investigated area than in southern populations. Based on pairwise Fst values, AMOVA results and PCo Analysis, moderate differentiation among population was detected, while the STRUCTURE analysis clearly separated SR-Kru and SR-Les. Data obtained for analyses of differentiation and gradual losses of genetic diversity of sampled populations provides useful information about invasion dynamics of common ragweed in recently invaded region.

Key words: Ambrosia artemisiifolia, Central Serbia, genetic diversity and structure, microsatellites

Corresponding author: Nataša Kočiš Tubić, Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia, Tel: +381 21 485 2656, Fax: +381 21 450 620, e-mail: natasa.kocis@dbe.uns.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 269-275

Serbia

UDC 575
DOI: 10.2298/GENSR1401269A

Original scientific paper

STUDY ON GENETIC VARIABILITY IN MHC-DRB1 SECOND EXON IN MAKUIE SHEEP BREED POPULATION

Fereshteh ASHRAFI¹, Ali HASHEMI¹, Karim MARDANI¹, Reza DARVISHZADEH¹

¹Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran.

²Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

³Department of Biotechnology, Urmia University, Urmia, Iran

Abstract

Ashrafi F., A.Hashemi, K. Mardani, and R. Darvishzadeh (2014): Study on genetic variability in MHC-DRB1 second exon in makuie sheep breed population. Genetika, Vol 46, No. 1, 269-275.

In the present study polymorphism of the exon 2 of MHC (Major Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2 fragment was amplified using polymerase chain reaction (PCR). PCR products were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR products were electrophoresed on 2% agarose gel. The results showed the existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the population under study. Effective number of alleles and heterozygosity for the examined region were 3.7231 and 0.7314, respectively. Chi-square test showed that the examined sheep population was not in Hardy-Weinberg equilibrium in the examined region.

Key words: genetic variation, Hardy-Weinberg equilibrium, Makuei sheep, PCR-RFLP, west Azerbaijan

Corresponding author: Fereshteh ASHRAFI, Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 277-286

Serbia

UDC 575
DOI: 10.2298/GENSR1401277S

Original scientific paper

COMPATIBILITY AND VERDURE OF SEEDLING FOR AUTOCHTONOUS PEER

IN THE REGION OF NORTH MONTENEGRO

Gordana ŠEBEK¹, Višnja KOVAČEVIĆ²

¹Biotechnical faculty. Department for continental fruit growing. University of Montenegro,

² Student of faculty of biology, direction of research. Beograd.

Abstract

Šebek G., and V. Kovačević (2014): Compatibility and verdure of seedling for autochtonous peer in the region of north Montenegro. Genetika, Vol 46, No. 1, 277-286.

The study was conducted in three successive years to examine the compatibility and verdure in nursery trees of autochthonous peer cultivars grafted on Quince MA and wild peer seedlings (Pyrus communis L) during the first year after bud grafting. The aim of this paper is to determine compatibility and verdure of autochthonous peer seedling sorts (Pećanka, Jerebasma, Lubeničarka, Sinka, Turšijača and Jarac) with the Quince MA and wild peer seedlings (Pyrus communis L). The severity of incompatibility varies between varieties but is always more severe with Quince MC than Quince MA.

The incompatibility expresses itself in several ways. Firstly a poor ‘bud’ or ‘graft’ take in the nursery will be evident, secondly even if growing successfully in the nursery very often when ‘lifted’ breakages (brittle unions) occur between rootstock and scion and thirdly ‘delayed’ incompatibility where again the union between rootstock and scion breaks suddenly in later years, this is unpredictable and can often happen when there is a heavy crop load assisted by strong autumnal winds.

The production of seedling material of autochthonous sorts with vegetative rootstocks Quince MA will be enormous contribution for even partially saving of fruit genofond that is the unity product of our ecological environment and autochthonous biocenosis.

Key words: autochthonous varieties, acceptance of grafting, growth dynamic, compatibility, morphological characteristics,one-year old seedlings,

Corresponding author: Gordana Šebek, PhD, Senior Research Fellow at Department for continental fruit growing, Biotechnical faculty, University of Montenegro, Ul. Mihaila Lalića No. 1, 81000 Podgorica, Montenegro; tel: +38269380183; fax: +38220268432; email: sebek@t-com.me

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Published in „GENETIKA“ Vol. 46, No.1(2014), pp. 287-301

Serbia

UDC 575
DOI: 10.2298/GENSR1401287B

Original scientific paper

STRAIN-SPECIFIC DIFFERENCES IN AGE-RELATED CHANGES IN RAT SUSCEPTIBILITY TO EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AND DENDRITIC CELL CYTOKINE GENE EXPRESSION

Biljana BUFAN¹, Jasmina DJIKIĆ², Mirjana NACKA-ALEKSIĆ², Zorica STOJIĆ-VUKANIĆ¹, Mirjana DIMITRIJEVIĆ³, Gordana LEPOSAVIĆ²

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

² Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

³Immunology Research Center “Branislav Janković”, Institute of Virology, Belgrade, Serbia

Abstract

Bufan B., J. Djikić, M. Nacka-Aleksić, Z. Stojić-Vukanić, M. Dimitrijević and G. Leposavić (2014): Strain-specific differences in age-related changes in rat susceptibility to experimental autoimmune encephalomyelitis and dendritic cell cytokine gene expression. Genetika, Vol 46, No. 1, 287 -301.

Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis, a prototype of Th1/Th17-mediated organ-specific autoimmune disease. In the rat, susceptibility to development of these diseases is shown to be strain- and age-dependent. In adult rats of distinct strains, it correlates with splenic dendritic cell (DC) subset composition, which also exhibit age-related changes. The aim of this study was to examine influence of aging on: i) Albino Oxford (relatively resistant to EAE) and Dark Agouti (susceptible to EAE) rat development of EAE and ii) their splenic conventional (OX62+) DC population in respect to its subset composition and expression of mRNAs for proinflammatory and immunosuppressive cytokines. We used 3-month-old (young) and 26-month-old (aged) rats of AO and DA strain. The rats were immunized for EAE with rat spinal cord homogenate in complete Freund’s adjuvant and clinical course of the disease was followed. Fresh OX62+DCs were examined for the expression of CD4 (using flow cytometry) and genes encoding cytokines influencing DC activation/maturation (TNF-α and IL-6) using RT-PCR. Additionally, in vitro lipopolysaccharide (LPS) activated/matured DCs were examined for the expression of genes encoding cytokines controlling Th1/Th17 cell polarization using RT-PCR. With aging, AO rats became more susceptible, whereas DA rats largely lose their susceptibility to the induction of EAE. In AO rats aging shifted CD4+:CD4- DC ratio towards CD4- cells, producing large amount of proinflammatory cytokines, whereas in DA rats CD4+:CD4- DC ratio remained stable with aging. In fresh DCs from rats of both the strains the expression of TNF-α mRNA increased with aging, whereas that of IL-6 mRNA decreased and increased in DCs from AO and DA rats, respectively. Following in vitro LPS stimulation OX62+ DCs from aged AO rats up-regulated the expression of mRNA for IL-23p19 (specific subunit of IL-23; crucial for sustained IL-17 production) and IL-1β (positive IL-17 regulator), whereas down-regulated the expression of IL-10 (negative IL-17 regulator) when compared with young strain-matched rats. In DA rats aging incresed IL-23p19 mRNA expression in LPS-stimulated DCs, whereas exerted the opposing effects on the expression of mRNAs for IL-10 and IL-1β compared to AO rats. Irrespective of the rat strain, aging did not influence mRNA expression for IL-12p35 (driving Th1 polarization) in DCs. Overall, results suggest role of changes in the expression of genes encoding proinflammatory and immunosuppressive cytokines in development of age-related alterations in rat susceptibility to EAE induction.

Key words: Aging, Cytokine gene expression, Dendritic cells, Experimental autoimmune encephalomyelitis, Rat strain differences

Corresponding author: Gordana Leposavić, Department of Physiology, Faculty of Pharmacy, University of Belgrade, 450 Vojvode Stepe, 11221 Belgrade, Serbia, Tel. +381 11 3951 209, E-Mail: Gordana.Leposavic@pharmacy.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 301-313

Serbia

UDC 575.630
DOI: 10.2298/GENSR1401301S

Original scientific paper

Morpho-chemical characterization of dry and snap bean (Phaseolus vulgaris L.) landraces collected on Fruška gora Mt.

Aleksandra SAVIĆ¹, Gordana PETROVIĆ¹, Mirjana MILOŠEVIĆ¹, Zorica NIKOLIĆ¹, Anamarija STOJANOVIĆ¹, Jelica GVOZDANOVIĆ-VARGA¹, Vida TODOROVIĆ², MirjanaVASIĆ¹

¹ Institute of Field and Vegetable Crops, Novi Sad, Serbia

² Faculty of Agriculture, Banja Luka

Abstract

Savić A., G. Petrović, M. Milošević, Z. Nikolić, A. Stojanović, J. Gvozdenović-Varga, V. Todorović and M. Vasić (2014): Morpho-chemical characterization of dry and snap bean (Phaseolus vulgaris L.) landraces collected on Fruška Gora MT..- Genetika, Vol 46, No. 1, 301-313.

Disappearance of old cultivars, including dry and snap bean (Phaseolus vulgaris L.) has been accelerated in last five to six decades, which mainly led to great genetic impoverishment. For all the humanity and its future, particularly is important the maintenance and evaluation of old cultivar’s seeds. The research presented in this paper has been conducted on the territory of southwestern Fruška gora Mt. Of the collected samples of field and vegetables crops, as well as wild plants on the mountain, 13 accessions of snap bean and 21 accessions of dry bean have been analyzed in this paper. Seed color, seed shape, 1000-seed mass and phaseolin type was determined for all the accessions. Seeds of collected bean and snap bean accessions were predominantly white and cylindrical in shape. Mass of 1000 seeds ranged between 104,90 g and 634,96 g. T phaseolin type dominated, while S type of phaseolin was present in six bean and in two snap bean accessions.

Key words: landraces, collection, main traits, phaseolin type, Phaseolus vulgaris

Corresponding author: Aleksandra Savić, Institute of Field and Vegetable Crops, Novi Sad, phone: +381214898357; +38121631936458, fax number: +381214898355, e-mail address: aleksandra.savic@nsseme.com

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Published in „GENETIKA“ Vol. 46, No. 1(2014), pp. 315-330

Serbia

UDC 575
DOI: 10.2298/GENSR1401315P
Review paper

CYTOGENETIC ALTERATIONS IN PERIPHERAL CELLS OF ALZHEIMER’S DISEASE PATIENTS

Bosiljka PLEĆAŠ-SOLAROVIĆ¹, Ninoslav ĐELIĆ², Vladan BAJIĆ³, Lada ŽIVKOVIĆ¹,

Biljana SPREMO-POTPAREVIĆ¹

¹Department of Physiology, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia

²Department of Biology, University of Belgrade, Faculty of Veterinary Medicine, Belgrade, Serbia

³Institute of Pharmaceutical Research, Galenika, Belgrade, Serbia

Abstract

Plečaš Solarević B., N. Đelić, V. Bajić, L. Živković, and B. Spermo-Potparević (2014): Cytogenetic alterations in peripheral cells of alzheimer’s disease patients.-Genetika, Vol 46, No. 1, 315-330.

Alzheimer’s disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid β protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.

Key words: Alzheimer’s disease, chromosome instability, peripheral cells

Corresponding author: Bosiljka Plećaš-Solarović, Faculty of Pharmacy,Department of Physiology, Vojvode Stepe 450, 11000 Belgrade, Serbia, Tel.: +381 11 3951346, Fax: +381 11 3972840, E-mail: bosiljka.plecas@pharmacy.bg.ac.rs

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