GENETIKA, Vol

GENETIKA, Vol. 47, No. 2 (2015)

Jelena BOŠKOVIĆ

In memoriam prof dr Janoš Berenji (1954-2015)

Kulpash BULATOVA, Raushan MASSONICHICH-SHOTUNOVA, Galiolla MeiIrman, Shynar Mazkirat

DIVERSITY OF SAINFOIN (Onobrychis Mill.) COLLECTION SAMPLES ACCORDING TO THE SPECTRA OF STORAGE PROTEINS [Abstract] [Full text]

Khosro MOHAMMADI and Reza TALEBI

INTERRELATIONSHIPS AND GENETIC ANALYSIS OF SEED YIELD AND MORPHOLOGICAL TRAITS IN MINI CORE COLLECTION OF IRANIAN LANDRACE, BREEDING LINES AND IMPROVED CHICKPEA (Cicer arietinum l.) CULTIVARS [Abstract] [Full text]

Natalia.A.ZINOVIEVA, Marina.I.SELIONOVA, Elena.A.GLADYR, Milan.P. PETROVIC Violeta. CARO PETROVIC, Dragana. RUZIC MUSLIC, Milan.M.PETROVIC

INVESTIGATION OF GENE POOL AND GENEALOGICAL LINKS BETWEEN SHEEP BREEDS OF SOUTHERN RUSSIA BY BLOOD GROUPS AND DNA MICROSATELLITES [Abstract] [Full text]

Gordana S TIMOTIJEVIĆ, Mira DJ MILISAVLJEVIĆ, Dragana B NIKOLIĆ, Boško M MILOVANOVIĆ, Dragana S NIKOLIĆ, Miroslav NIKOLIC and Jelena T SAMARDŽIĆ
ESTABLISHMENT AND IN-HOUSE VALIDATION OF STEM-LOOP RT PCR METHOD FOR MICRORNA398 EXPRESSION ANALYSIS [Abstract] [Full text]

Zahra ERFANIASL, Ali HASHEMI, Ghorban Elyasi ZARRINGHABAIE, Mohammad FARHADIAN

ASSOCIATION OF THE INTERLEUKIN-2 GENE POLYMORPHISM WITH EGG PERFORMANCE IN A NATIVE TURKEY POPULATION [Abstract] [Full text]

Li-jing CHEN, Hong-Mei LI , Shao-kun SUN, Muhammad IRFAN, Jing-wei LIN, Ming ZHONG, Hui MA, Zhi-Fu GUO, Tian-lai LI

CONSTRUCTION OF A GENETIC LINKAGE MAP IN Lilium USING A RIL MAPPING POPULATION BASED ON SRAP MARKER [Abstract] [Full text]

Svetlana VOJVODIĆ and D. ADEMOVIĆ-SAZDANIĆ

KILLER-CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES LINKAGE DISEQUILIBRIUM ANALYSIS IN POPULATION OF VOJVODINA [Abstract] [Full text]

Malahat TABAN, Masoud SHEIDAI, Zahra NOORMOHAMMADI, Narjes AZIZI, Somayeh GHASEMZADEH-BARAKI, Fahimeh KOOHDAR

POPULATION GENETIC ANALYSIS AND EVIDENCE OF INTER-SPECIFIC INTROGRESSION IN Helichrysum armenium AND H. rubicundum (Asteraceae) [Abstract] [Full text]

Iva PRUNER, Valentina DJORDJEVIC, Maja GVOZDENOV, Branko TOMIC, Mirjana KOVAC, Predrag MILJIC, Dragica RADOJKOVIC

THE C20068T GENE VARIANT IN THE 3` END OF THE PROTHROMBIN GENE AND RECURRENT PREGNANCY LOSS: A PILOT STUDY [Abstract] [Full text]

Hongmei SONG, Yingchen WEI, Nan LI, Bin WU, Na XIE, Heming WANG

WENYANGBUSHEN-INDUCED-EXPRESSION OF VEGF, OPG, RANK, AND RANKL IN RABBITS WITH STEROID-INDUCED FEMORAL HEAD AVASCULAR NECROSIS [Abstract] [Full text]

Ana NIKOLIĆ, Dragana IGNJATOViĆ-MICIĆ, Dragan KOVAČEVIĆ, Zoran ČAMDŽIJA, Milomir FILIPOVIĆ, Snežana MLADENOVIĆ DRINIĆ
GENETIC DIVERSITY OF MAIZE INBRED LINES AS INFERRED FROM SSR MARKERS [Abstract] [Full text]

Abbas DOOSTI, Payam GHASEMI-DEHKORDI, Mohammad KARGAR, Abolghasem SHARIFI

GENERATION OF DIVALENT DNA VACCINE BASED ON p39 AND shiga-like toxin 2 (stx2) GENES [Abstract] [Full text]

Željko LAKIĆ, Igor BALALIĆ, Svetko VOJIN

Interpretation of Genotype × Environment interaction in Perennial ryegrass (Lolium perenne L.) [Abstract] [Full text]

M. YASODHA and P. PONMUTHURAMALINGAM

A MICROARRAY GENE EXPRESSIONS WITH CLASSIFICATION USING EXTREME LEARNING MACHINE [Abstract] [Full text]

Parul BANERJEE and Bashisth Narayan SINGH

STUDIES ON FLUCTUATING ASYMMETRY (FA) FOR CERTAIN MORPHOLOGICAL TRAITS IN FOUR SPECIES OF THE Drosophila bipectinata COMPLEX [Abstract] [Full text]

Ahmad Reza GOLPARVAR, Mohammad Mehdi GHEISARI, Davoud NADERI, Ali Mehras MEHRABI, Amin HADIPANAH, Saeed SALEHI

DETERMINATION OF THE BEST INDIRECT SELECTION CRITERIA IN IRANIAN DURUM WHEAT (Triticum aestivum L.) GENOTYPES UNDER IRRIGATED AND DROUGHT STRESS CONDITIONS [Abstract] [Full text]

Mehrzad ALLAHGHOLIPOUR, Ezatollah FARSHDFAR, Babak RABIEI

COMBINING ABILITY AND HERITABILITY OF SELECTED RICE VARIETIES FOR GRAIN YIELD, ITS COMPONENTS AND GRAIN QUALITY CHARACTERS [Abstract] [Full text]

Vladan POPOVIĆ, Aleksandar LUČIĆ, Danijela RISTIĆ, Ljubinko RAKONJAC, Sabahudin HADROVIĆ, Snežana MLADENOVIĆ DRINIĆ

ANALYSIS OF INTRA-POPULATION VARIABILITY OF BALD CYPRESS (Taxodium distichum L. RICH.) IN SEED STAND NEAR BACKA PALANKA USING RAPD MARKERS [Abstract] [Full text]

Esmaeel GHOLINEZHAD, Reza DARVISHZADEHandIraj BERNOUSI

EVALUATION OF SUNFLOWER GRAIN YIELD COMPONENTS UNDER DIFFERENT LEVELS OF SOIL WATER STRESS IN AZERBAIJAN [Abstract] [Full text]

Alena GAJDOŠOVÁ, Tatjana VUJOVIĆ, Miroslava SÚKENÍKOVÁ,Gabriela LIBIAKOVÁ

IMPROVEMENT OF ADVENTITIOUS ORGANOGENESIS FOR REGENERATION OF TRANSGENIC PLANTS IN BLACKBERRY [Abstract] [Full text]

Klaudija DAUGĖLAITĖ and Danielius SERAPINAS

THE IMPORTANCE OF MTHFR GENE MUTATION DETECTION IN PATIENT WITH RECURRENT MISCARRIAGES [Abstract] [Full text]

Afet MAMMADOVA, Ramiz ALIYEV, Sevda BABAYEVA, Mehraj ABBASOV

ADAPTIVE REACTION OF COTTON ACCESSIONS OF G. hirsutum L. AND G. barbadense L. SPECIES TO DROUGHT STRESS [Abstract] [Full text]

Dejana SAVIC, Dragana JOSIC, Elizabeta RISTANOVIC, Radmila PIVIC, Aleksandra STANOJKOVIC-SEBIC, Zorica LEPSANOVIC

DETECTION OF TOXIN GENES AND RAPD ANALYSIS OF Bacillus cereus ISOLATES FROM DIFFERENT SOIL TYPES [Abstract] [Full text]

Lydia Shtereva, Roumiana Vassilevska-Ivanova, Boris Kraptchev

Evaluation of drought tolerance in new bulgarian sweet corn genotypes with using stress tolerance indices [Abstract] [Full text]

Dragana MILOŠEVIĆ, Ivana STANKOVIĆ, Aleksandra BULAJIĆ, Maja IGNJATOV, Zorica NIKOLIĆ, Gordana PETROVIĆ, Branka KRSTIĆ

DETECTION AND MOLECULAR CHARACTERIZATION OF Pepper mild mottle virus IN SERBIA [Abstract] [Full text]

M. MOAEEN-UD-DIN, G. BILAL and James M. REECY

EVOLUTION OF HYPOTHALAMUS-PITUITARY GROWTH AXIS AMONG FISH, AMPHIBIAN, BIRDS AND MAMMALS [Abstract] [Full text]

Uroš ŽIVKOVIĆ, Danijela MILJKOVIĆ, Nataša BARIŠIĆ KLISARIĆ, Aleksej TARASJEV, Stevan AVRAMOV

PERFORMANCE OF Iris variegata GENOTYPES IN DIFFERENT LIGHT CONDITIONS: FLOWERING PHENOLOGY AND REPRODUCTIVE OUTPUT [Abstract] [Full text]

Sandra PETROVIC, Andreja LESKOVAC, Ivana JOKSIC, Dragana VUJIC, Ana Valenta SOBOT, Jelena FILIPOVIC and Gordana JOKSIC

ASSESSMENT OF SINGLE NUCLEOTIDE POLYMORPHISMS IN SCREENING 52 DNA REPAIR AND CELL CYCLE CONTROL GENES IN FANCONI ANEMIA PATIENTS [Abstract] [Full text]

F. IZADPANAH, S. KALANTARI, M. E. HASSANI, M. R. NAGHAVI, M. SHOKRPOUR

MOLECULAR AND MORPHOLOGICAL VARIATION IN SOME IRANIAN SAFFRON (Crocus sativus L.) ACCESSIONS [Abstract] [Full text]

Milena CVETKOVIĆ, and Perica VASILJEVIĆ

HANDEDNESS AND PHENOTYPIC CHARACTERISTICS OF THE HEAD AND FACE [Abstract] [Full text]

Sahar GOHARI-LASAKI, Jalal GHARESOURAN, Morteza GHOJAZADEH,Vahid MONTAZERI, Hakimeh SAADATIAN, Seyyed Mojtaba Mohaddes ARDEBILI

DNA REPAIR GENE XRCC3 241MET VARIANT AND BREAST CANCER SUSCEPTIBILITY OF AZERI POPULATIONIN IRANIAN [Abstract] [Full text]

^{Khaled F. M.
Salem}

ALLELIC STATE AT THE MICROSATELLITE LOCUS XGWM261 MARKING THE DWARFING GENE RHT8 IN EGYPTIAN BREAD WHEAT (Triticum aestivum L.) GENOTYPES RELEASED FROM 1947 TO 2004 [Abstract] [Full text]

Milan MIROSAVLJEVIĆ, Novo PRŽULJ, Vojislava MOMČILOVIĆ, Nikola HRISTOV, Ivana MAKSIMOVIĆ

DRY MATTER ACCUMULATION AND REMOBILIZATION IN WINTER BARLEY

AS AFFECTED BY GENOTYPE AND SOWING DATE [Abstract] [Full text]

Suzana CVJETICANIN

ANTHROPOGENETIC VARIABILITY IN THE GROUPS OF HOMO- AND HETEROSEXUALLY ORIENTED INDIVIDUALS [Abstract] [Full text]

Correction:

Popović V., A. Lučić, M. Šijačić-Nikolić, T. Ćirković-Mitrović, Lj. Rakonjac, B. Cvetković and S. Mladenović Drinić (2014): Analysis of inter-line variability of bald cypress (Taxodium distichum L. Rich.) juvenile seedlings using morphometric markers-. Genetika, Vol 46, No. 1, 117-128. [Correction]

Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 375-382

UDC 575.630
DOI: 10.2298/GENSR1502375B

Orginal scientific paper

DIVERSITY OF SAINFOIN (Onobrychis Mill.) COLLECTION SAMPLES ACCORDING TO THE SPECTRA OF STORAGE PROTEINS

Kulpash BULATOVA¹ *, Raushan MASSONICHICH-SHOTUNOVA²,

Galiolla MeiIrman¹, Shynar Mazkirat¹

¹Kazakh Research Institute of Agriculture and Crop Growing,

Almalybak, Karasay district, Almaty region, Republic of Kazakhstan

²Kazakh Research Institute of Animal Husbandry and Forage Production,

Almaty, Republic of Kazakhstan

Abstract

Bulatova K., R. Massonichich-Shotunova, G. Meiirman, S. Mazkirat (2015): Diversity of sainfoin (Onobrychis Mill.) collection samples according to the spectra of storage proteins. - Genetika, Vol 47, No. 2, 375 -382.

Sainfoin (Onobrychis Mill.) is a valuable forage plant of the legume family (Fabaceae). In Kazakhstan the sainfoin introduced as field fodder in the 50s of the XX century and because of its biological features widely spread as a promising crop for forage resources increasing. The electrophoresis of seed storage proteins of Onobrychis viciifolia, Onobrychis arenaria and Onobrychis transcaucasica species was carried out. Considerable diversity of the number, mobility and intensity of components was determined.

Key words: diversity, electrophoresis, sainfoin, seed proteins

Corresponding author: Kulpash Bulatova, Laboratory of molecular-biological analyses of plants, Kazakh Research Institute of Agriculture and Crop Growing, 040909, Erlepesov Street 1, v. Almalybak, Karasay district, Almaty region, Republic of Kazakhstan, Phone: +7 72771 53 130. E-mail: bulatova_k@rambler.ru

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Published in „GENETIKA“ Vol. 7, No. 2 (2015), pp. 383 -393

Serbia

UDC 575.633
DOI:10.2298/GENSR1502383M

Original scientific paper

INTERRELATIONSHIPS AND GENETIC ANALYSIS OF SEED YIELD AND MORPHOLOGICAL TRAITS IN MINI CORE COLLECTION OF IRANIAN LANDRACE, BREEDING LINES AND IMPROVED CHICKPEA (Cicer arietinum l.) CULTIVARS

Khosro MOHAMMADI and Reza TALEBI^*

College of Agriculture, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran

Abstract

Mohammadi K. and R. Talebi (2015): Interrelationships and genetic analysis of seed yield and morphological traits in mini core collection of Iranian landrace, breeding lines and improved chickpea (Cicer arietinum L.) cultivars. - Genetika, Vol 47, No. 2,383-393.

To determine the association between genetic parameters and morphological traits in chickpea (Cicer arietinum L.) genotypes, a field experiment was conducted with 49 different landrace, breeding lines and cultivated chickpea genotypes using a 7×7 lattice square design with 2 replications in the 2012-2013 growing season. Genetic parameters including genetic, environmental and phenotypic variances; coefficients of variation; heritability; correlation coefficients; factor analysis and path coefficients were estimated, and cluster analysis was performed. High heritability values observed in measured traits indicating that these traits are controlled mainly by additive genes and that selection of such traits may be effective for improving seed yield. Number of seeds per plant, 100-seed weight and plant biomass had a positive direct effect on seed yield. These traits also had positive and highly significant phenotypic correlations with seed yield. Using principal component (PC) analysis, the first three PCs with eigenvalues more than 1 contributed 70.94% of the variability among accessions. The germplasm were grouped into 3 clusters. Each cluster had some specific characteristics of its own and the cluster I was clearly separated from cluster II and III. Overall the results, it can be concluded that seed yield in chickpea can be improved by selecting an ideotype having greater number of seeds per plant, 100-seed weight and plant biomass.

Key words: chickpea, cluster analysis, heritability, variance components,

Corresponding author: Reza Talebi, College of Agriculture, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran, email: srtalebi@yahoo.com

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Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 395- 404

Serbia

UDC 575.630
DOI: 10.2298/GENSR1502395Z

Original scientific paper

INVESTIGATION OF GENE POOL AND GENEALOGICAL LINKS BETWEEN SHEEP BREEDS OF SOUTHERN RUSSIA BY BLOOD GROUPS AND DNA MICROSATELLITES

Natalia.A.ZINOVIEVA¹, Marina.I.SELIONOVA², Elena.A.GLADYR¹, Milan.P. PETROVIC³, Violeta. CARO PETROVIC³, Dragana. RUZIC MUSLIC³, Milan.M.PETROVIC³

¹All-Russian Research Institute of Animal Husbandry, Moscow, Russia

²All-Russian Research Institute of Sheep and Goat, Stavropol, Russia

³Institute for Animal Husbandry, Belgrade-Zemun, Serbia

Abstract

Zinovieva, N..A., M..I.Selionova, E..A.Gladyr, M.P. Petrovic, V. Caro Petrovic, D. Ruzic Muslic, M..M.Petrovic (2015): Investigation of gene pool and genealogical links between sheep breeds of southern Russia by blood groups and DNA microsatellites.- Genetika, Vol 47, No. 2, 395 - 404.

To study the gene pool and the establishment of genealogical relationships between breeds of sheep of different directions productivity bred in Russia, were used two classes of genetic markers - blood and DNA microsatellites. The included sample sheep are fine-wool Merino breeds: Grozny (GR), Caucasian (CA), Manychskij merino (MM), the Soviet Merino (SM), Stavropol (ST) and coarse wool breeds: Edilbaevskaya (ED), Karakul (CR) and Romanov (RO). For the study of erythrocyte, were selected antigens (blood group) in 1159 samples from 11 breeding farms. For microsatellite DNA study - 598 from 10 breeding farms. Microsatellite analysis revealed that the most polymorphic were Stavropol breed sheep that have identified an average of 18.27 alleles per locus were relatively conservative Romanov breed sheep - 9.7 alleles per locus. The minimum genetic distances established between Grozny and Soviet Merino - 0.0569 (for microsatellites) and 0.0741 (blood groups - later in the same sequence). The rocks of the Stavropol – Grozny were 0.0861 and 0, 0810. Whereas Stavropol and Soviet Merino 0.0861 and 0.1094. Also relatively close between Grozny – Edilbaevskoy , Grozny - Karakul, Edilbaevskoy - Karakul: 0.1364 and 0.0851, respectively; 0.1620 and 0.1208; 0.1875 and 0.1192. The highest genetic distances were between Stavropol and Karakul - 0.2664 and 0.1804, as well as between the Romanov and all studied species - 0.2491 ... 0.3211 and 0.1734 ... 0.2235.

Key words: blood group, DNA microsatellites, genetic markers, genealogical analysis, polymorphism, sheep

Corresponding author: Milan P. Petrovic, Institute for Animal Husbandry, Belgrade-Zemun, Serbia

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Published in „GENETIKA“ Vol. 47, No2 (2015), pp. 405 -416

Serbia

UDC 575
DOI: 10.2298/GENSR1502405T

Original scientific paper

ESTABLISHMENT AND IN-HOUSE VALIDATION OF STEM-LOOP RT PCR METHOD FOR MICRORNA398 EXPRESSION ANALYSIS

Gordana S TIMOTIJEVIĆ¹*, Mira DJ MILISAVLJEVIĆ¹, Dragana B NIKOLIĆ¹, Boško M MILOVANOVIĆ², Dragana S NIKOLIĆ¹, Miroslav NIKOLIC³ and Jelena T SAMARDŽIĆ¹

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia,

²Geographical Institute“Jovan Cvijić”, Serbian Academy of Sciences and Arts, Belgrade, Serbia and

³Plant Nutrition Research Group, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia

Abstract

Timotijević G., M. Milisavljević, D. Nikolić, B. Milovanović, D. Nikolić, M.Nikolic and J. Samardžić (2015): Establishment and in-house validation of stem-loop RT PCR method for microrna398 expression analysis. - Genetika, Vol 47, No. 2, 405-416.

MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by “stem-loop” primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for “stem loop” primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as “stem loop” RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.

Key words: miRNA gene expression analysis, Real time PCR, iron deficiency

Corresponding author: Gordana Timotijević, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, VojvodeStepe 444a, PO box 23, 11010 Belgrade, Serbia,Fax: +381113976414, e-mail: timotijevic@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 47, No.2 (2015), pp. 417 -424

Serbia

UDC 575
DOI: 10.2298/GENSR1502417E

Original scientific paper

ASSOCIATION OF THE INTERLEUKIN-2 GENE POLYMORPHISM WITH EGG PERFORMANCE IN A NATIVE TURKEY POPULATION

Zahra ERFANIASL^1*, Ali HASHEMI¹, Ghorban Elyasi ZARRINGHABAIE²,

Mohammad FARHADIAN³

¹Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran.

²Scientific member of Agriculture and Natural resources Research Center of East Azarbaijan Province, Tabriz, Iran.

³Young Researchers and Elite Club, Khoy Branch, Islamic Azad University, Khoy, Iran.

Abstract

Erfaniasl Z., A. Hashemi, G. Elyasi Zarringhabaie, M. Farhadian (2015): Association of the interleukin-2 gene polymorphism with egg performance in a native Turkey population- Genetika, Vol 47, No. 2, 417-424.

Interleukin-2 (IL2) is a powerful growth factor for a variety of cell types, including T-cells, and therefore plays a crucial role in immune system's functioning. Polymorphism of the Interleukin-2 gene in west Azerbaijan native turkey was investigated using PCR and single-stranded conformation polymorphism technique (SSCP). Genomic DNA of 185 turkey samples was isolated from whole blood. A 523-bp IL2 second exon with part of the third intron segment was amplified by standard PCR, using locus-specific primers. Three SSCP patterns, representing three different genotypes, were identified. The frequencies of the genotypes were 13.83%, 8.51% and 77.66 % for AA, BB and AB, respectively. Allele frequencies were 52.65% and 47.35 %for A and B respectively. Observed heterozygosity was 0.6196. There was a significant deviation from the Hardy-Weinberg equilibrium for this locus. Relationships between molecular polymorphism in the second exon with part of the third intron regions of Interleukin-2 and number of eggs, average weight of eggs (g) and weight of egg mass production (g) were investigated. The results showed that the AA genotype was associated with the number of eggs and Weight of egg mass production (g) (P ≤ 0.01). No correlation between the second exon and part of the third intron Interleukin-2 variants with the average weight of eggs was found (g). We expect that this gene could be a candidate for the genetic improvement of some economic traits in turkey breeding programs

Key words: Genetic Polymorphism, IL-2, Turkey, SSCP

Corresponding author: Mohammad Farhadian,Young Researchers and Elite Club, Khoy Branch, Islamic Azad University, Khoy, Iran,E-mail: Mohammad.farhadian@tabrizu.ac.ir

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Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 425 -438

Serbia

UDC 575:630
DOI: 10.2298/GENSR1502425C

Original scientific paper

CONSTRUCTION OF A GENETIC LINKAGE MAP IN Lilium USING A RIL MAPPING POPULATION BASED ON SRAP MARKER

Li-jing CHEN^#*, Hong-mei LI^# , Shao-kun SUN^#, Muhammad IRFAN, Jing-wei LIN, Ming ZHONG, Hui MA, Zhi-Fu GUO, Tian-lai LI*

Key Laboratory of Agricultural Biotechnology of Liaoning Province, Key Laboratory of Protected Horticulture (Ministry of Education), Bioscience and Biotechnology College, Shenyang Agriculture University, Shenyang 110866 China

Abstract

Chen L., H.Li , S. Sun, M. Irfan, J. Lin, M. Zhong, H. Ma, Z. Guo, T. Li (2015): Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker- Genetika, Vol 47, No. 2, 425- 438.

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.

Key words: genetic linkage map, genome length, Lily, map coverage, RIL, SRAP

Corresponding author: Li-jing Chen, E-mail: chenlijing1997@126.com, Phone: +86-24-88487163, Fax: +86-24-88492799.Tian-lai Li，E-mail: litianlai@126.com, Phone: +86-24-88487143, Fax: +86-24-88487143

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Published in „GENETIKA“ Vol. 47, No.2 (2015), pp.439 -450

Serbia

UDC 575
DOI: 10.2298/GENSR1502439V

Original scientific paper

KILLER-CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES LINKAGE DISEQUILIBRIUM ANALYSIS IN POPULATION OF VOJVODINA

Svetlana VOJVODIĆ and D. ADEMOVIĆ-SAZDANIĆ

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, University of Novi Sad, Medical faculty Novi Sad

Abstract

Vojvodić S. and D. Ademović-Sazdanić (2015): Killer-cell immunoglobulin-like receptor genes linkage disequilibrium analysis in population of Vojvodina- Genetika, Vol 47, No. 2, 439-450.

Killer Immunoglobulin-like Receptors (KIRs) form a group of regulatory molecules that modulate cytolytic activity of natural killer cells and T cells through interaction with specific human leukocyte antigen (HLA) molecules on target cells. KIRs are encoded by the family of 16 homologous genes that vary substantially between haplotypes and display sequence polymorphism with allelic variation that also contributes to diversity within the complex. The aim of the study is to estimate two locus linkage disequilibrium for 16 KIR loci. In this study, we report the evaluation of KIR gene content, allele, haplotype and genotype frequencies in 175 unrelated healthy individuals from Vojvodina who were KIR typed by polymerase chain reaction-sequence specific primers genotyping assay. The linkage disequilibrium (LD) was studied at the structural level (presence or absence of 16 KIR genes). Our results revealed that linkage disequilibrium is present between telomeric gene pairs KIR2DL1~KIR2DL4, KIR2DP1~KIR2DL4, KIR2DP1~KIR3DL1, KIR2DL1~KIR3DL2, KIR2DP1~KIR3DL2, KIR2DL4~KIR3DL1, KIR2DL4~KIR2DS4, KIR2DL4~KIR3DL2 where (r²=1), but positive association between KIR genes, with higher observed than expected haplotype frequencies were observed for KIR3DS1~KIR2DS1 and KIR2DL5~KIR2DS1 pair of genes (r²=0.646) and (r²=0.371), respectively. Thirty-eight different genotypes were identified, where 12% of the individuals have unique genotype, present in only one person. Our results will help to understand the genetic background of the Vojvodina population, in illustrating the population migration events in the northern part of Serbia, in explaining the extensive genetic admixture amongst the different ethnic groups of the region and also in KIR-related disease studies.

Key words: KIR genes, Linkage disequlibrium

Corresponding author: Svetlana Vojvodić, M.D., Ph.D., Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Compartment, Hajduk Veljkova 9a, 21000 Novi Sad, Serbia; Tel.: +381-21-4877-963; Fax: +381-21-4877-978; Mobile: +381-64-861-58-12; E-mail: svetlana.vojvodic021@gmail.com

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Published in „GENETIKA“ Vol. 47, No.2 (2015), pp. 451-468

Serbia

UDC 575:630
DOI: :10.2298/GENSR1502451T

Original scientific paper

POPULATION GENETIC ANALYSIS AND EVIDENCE OF INTER-SPECIFIC INTROGRESSION IN Helichrysum armenium AND H. rubicundum (Asteraceae)

Malahat TABAN¹, Masoud SHEIDAI¹, Zahra NOORMOHAMMADI^2*, Narjes AZIZI¹,

Somayeh GHASEMZADEH-BARAKI¹, Fahimeh KOOHDAR¹

¹Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran

²Department of Biology, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Taban M,, M. Sheidai, Z. Noormohammadi, N. Azizi, S. Ghasemzadeh-Baraki, F. Koohdar (2015): Population genetic analysis and evidence of inter-specific introgression in Helichrysum armenium and H. rubicundum (Asteraceae). - Genetika, Vol 47, No. 2, 451-468.

Helichrysum armenium and H. rubicundum are two medicinally important plants of Iran that are distributed in various regions of the country. They are extensively used by locals as medicinal plants and a great negative selection pressure is applied on them. Therefore, due to importance of these plant species, we performed a population genetic study in both species. For this study, we used 66 randomly collected plants from 24 geographical populations of Helichrysum armenium and H. rubicundu. These species had areas of overlap and contact and we found some intermediate plants that were included in our study too. UPGMA and MDS analyses revealed morphological separation of these closely related species and placed intermediate plants in an intermediate position. ISSR analysis revealed inter-population genetic diversity and K-Means clustering and STRUCTURE analyses revealed populations^. genetic stratification in both species. Genetic difference of the studied populations was not correlated to geographical distance. Triangle plot of Bayesian analysis and NeighborNet plot showed inter-specific gene flow. The studied populations showed plants with 2n = 2x = 14 and 2n = 4x = 28 chromosomes and differed significantly in their meiotic behavior. Therefore, a combination of genetic stratification, and genetic admixture as well as polyploidy and chromosomes structural changes, have played rule in Helichrysum diversification.

Key words: genetic admixture, Helichrysum, polyploidy,. Inter-specific gene flow

Corresponding author: Zahra Noormohammadi, Department of Biology, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran,,Tel/Fax: +98 21 44865939,Email address: marjannm@yahoo.com z-nouri@srbiau.ac.ir

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Published in „GENETIKA“ Vol. 47, No.2 (2015), pp. 469-476

Serbia

UDC 575
DOI: 10.2298/GENSR1502469P

Original scientific paper

THE C20068T GENE VARIANT IN THE 3` END OF THE PROTHROMBIN GENE AND RECURRENT PREGNANCY LOSS: A PILOT STUDY

Iva PRUNER¹, Valentina DJORDJEVIC¹, Maja GVOZDENOV¹, Branko TOMIC¹,

Mirjana KOVAC^2,3, Predrag MILJIC^2,4, Dragica RADOJKOVIC¹

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

²Faculty of Medicine, University of Belgrade, Belgrade, Serbia,

³Blood Transfusion Institute of Serbia, Hemostasis Department, Belgrade, Serbia

⁴Clinic of Hematology, University Clinical Center, Belgrade, Serbia

Abstract

Pruner I., V. Djordjevic, M. Gvozdenov, B. Tomic, M. Kovac, P. Miljic, D..Radojkovic (2015): The c20068t gene variant in the 3` end of the prothrombin gene and recurrent pregnancy loss: a pilot study. - Genetika, Vol 47, No. 2, 469-476.

Recurrent pregnancy loss (RPL) is a health problem affecting up to 5% of women of reproductive age. Several thrombophilic risk factors might contribute to RPL.To investigate relationship between a novel C20068T gene variant in the 3` end of prothrombin gene and RPL, we tested 153 women with RPL and 111 controls for the presence of this gene variant. In patients, we have detected four heterozygous (2.61%) and no homozygous carriers. In controls, no carriers were detected. Our results indicate higher prevalence of C20068T gene variant in women with RPL but this difference was not statistically significant. However, in patients who suffered 5 or more RPL, frequency of C20068T gene variant was significantly increased compared to controls (12.5% vs. 0%, P=0.02).

This is the first study which points out a possible role of C20068T gene variant in etiology of RPL, but larger studies should be carried out to confirm our findings.

Key words: recurrent pregnancy loss, 3` end of the prothrombin gene, synonymous gene variant,thrombophilia

Corresponding author: Iva Pruner,Laboratory for Molecular Biology,Institute of Molecular Genetics and Genetic Engineering,Vojvode Stepe 444a,P.O. Box 23; 11010 Belgrade, Serbia,Phone: +381 11 3976658,Fax: +381 11 3975808,e-mail: iva.pruner@gmail.com

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Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 477 - 488

Serbia

UDC 575

DOI: 10.2298/GENSR1502477S

Original scientific paper

WENYANGBUSHEN-INDUCED-EXPRESSION OF VEGF, OPG, RANK, AND RANKL IN RABBITS WITH STEROID-INDUCED FEMORAL HEAD AVASCULAR NECROSIS

Hongmei SONG^1,2, Yingchen WEI², Nan LI², Bin WU¹, Na XIE³, Heming WANG^2,*

¹Internal Medicine Department Department, Second Affiliated People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China

²Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China

³Halison International Peace Hospital, Hengshui, Hebei, China

Abstract

Song H., Y. Wei, N. Li, B. Wu, N. Xie, H. Wang (2015): Wenyangbushen-induced-expression of VEGF, OPG, RANK, and RANKL in rabbits with steroid-induced femoral head avascular necrosis- Genetika, Vol 47, No. 2, 477-488.

This study was aimed to investigate the effects of Wenyangbushen formula on the mRNA and protein expression of VEGF, OPG, RANK, and RANKL in the steroid-induced avascular necrosis of the rabbit femoral head (SANFH) and to further explore the potential mechanism of this formula on the treatment of SANFH. One hundred and thirty six New Zealand rabbits were randomly divided into 5 groups (n=24 rabbits in each group): normal group, model group, Traditional Chinese Medicine (TCM) Wenyangbushen decoction at low, moderate and high dose group. The normal group and positive control group were intragastrically (i.g.) administered with saline. The TCM group was treated with Wenyangbushen decoction at the indicated dosage. After treated for eight weeks, the mRNA and protein expression of VEGF, OPG, RANK, and RANKL in the femoral head tissues was determined by RT-PCR and Western blotting, respectively. Wenyangbushen decoction treatment can effectively promote bone cells, osteoblasts and chondrocytes growth, as well as prevent the cell apoptosis in SANFH. The mRNA and protein expression of OPG and VEGF was increased while the levels of RANK and RANKL were reduced in the necrotic tissue of model group compared with those in the normal rabbits. And Wenyangbushen treatment prevented those changes, as manifested by up-regulation of VEGF and OPG, while down-regulation of RANK and RANKL levels in a dose-dependent manner. Wenyangbushen Formula treatment can alleviate necrosis of femoral head induced by steroid. It can promote bone cells, osteoblasts and chondrocytes growth, as well as prevent the cell apoptosis. Meanwhile, it up-regulates OPG and VEGF while inhibits RANK and RANKL expression. This may be one of the mechanisms for the effective treatment of SANFH.

Key words: Steroid-induced avascular Necrosis of the Femoral Head, Wenyangbushen Formula,VEGF,OPG-RANK-RANKLSignaling pathways

Corresponding author: Heming Wang,Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China E-mail: hemingwangfj@gmail.com

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Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 489- 498

Serbia

UDC 575:633.15

DOI: 10.2298/GENSR1502489N

Original scientific paper

GENETIC DIVERSITY OF MAIZE INBRED LINES AS INFERRED

FROM SSR MARKERS

Ana NIKOLIĆ, Dragana IGNJATOViĆ-MICIĆ, Dragan KOVAČEVIĆ, Zoran ČAMDŽIJA, Milomir FILIPOVIĆ, Snežana MLADENOVIĆ DRINIĆ

Maize Research Institute “Zemun Polje”, Belgrade, Serbia

Abstract

Nikolić A., D. Ignjatović-Micić, D. Kovačević, Z. Čamdžija, M. Filipović, S. Mladenović Drinić (2015): Genetic diversity of maize inbred lines as inferred from SSR markers- Genetika, Vol 47, No. 2, 489-498.

Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard’s coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.

Key words: genetic relatedness, inbred lines, maize, SSR markers

Corresponding author: Ana Nikolić, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, E-mail:anikolic@mrizp.rs, Phone: + 381 64 840 6216

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Published in „GENETIKA“ Vol. 47, No.2 (2015), pp. 499 -507

Serbia

UDC 575
DOI: 10.2298/GENSR1502499D
Original scientific paper

GENERATION OF DIVALENT DNA VACCINE BASED ON p39 AND shiga-like toxin

2 (stx2) GENES

Abbas DOOSTI¹, Payam GHASEMI-DEHKORDI²^*, Mohammad KARGAR³,

Abolghasem SHARIFI⁴

¹Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

²Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

³Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran

⁴ Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Doosti A., P. Ghasemi-Dehkordi, M. Kargar, A.Sharifi (2015): Generation of divalent DNA vaccine based on p39 and shiga-like toxin 2 (stx2) gene- Genetika, Vol 47, No. 2, 499-507.

The virulence factors such as shiga-like toxin (Stx) and immunogenic P39 protein in Escherichia coli and Brucella melitensis are related to disease of digestive system in human worldwide. In the present study the stx2 and p39 genes were cloned into expression plasmid pEEF1D-FLAG (pcDNA 3.1⁺) as a divalent DNA vaccine candidate. The Enterohemorrhagic E. coli ATCC 3081 and smooth virulent B. melitensis strain M5 were obtained and cultured on specific media. Bacterial DNA was extracted from colonies and was used for p39 and stx2 genes amplification by PCR. The amplified products on 2% agarose gel electrophoresis were revealed 285 and 1220 bp fragments for stx2 and p39 genes, respectively. Each amplified genes were T/A cloned into pGEM-T easy vector and pGEM-T-stx2 and pGEM-T-p39 were produced. The stx2 and p39 genes were sub-cloned in linearized expression vector (pcDNA 3.1⁺) using HindIII, XhoI and XbaI restriction enzymes and pCDNA3-stx2-p39 was generated. This final construct was confirmed by PCR and enzymes digestion. The results were showed stx2 and p39 genes were sub-cloned, successfully into pcDNA 3.1⁺ to generate pcDNA 3.1⁺-stx2-p39 recombinant vector. According to these findings novel recombinant pcDNA 3.1⁺-stx2-p39 construct that was produced in this study could be useful as DNA vaccine candidate in animal models against shiga-like toxin producing E. coli and virulence B. melitensis strains in future studies.

Key words: Brucella melitensis, Cloning, Escherichia coli, P39, PCR, Stx2

Corresponding author: Payam Ghasemi-Dehkordi, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran, Postal code: 8813833435,E-mails: payamghasemidehkordi@yahoo.com, biotechnologyskums@gmail.com

Tell: +98-38-33346692. Fax: +98-38-33330709.

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Published in „GENETIKA“ Vol. 47, No. 2 (2015), pp. 509-522

Serbia

UDC 575.633
DOI: 10.2298/GENSR1502509L
Original scientific paper

Interpretation of Genotype × Environment interaction

in Perennial ryegrass (Lolium perenne L.)

Željko LAKIĆ¹, Igor BALALIĆ², Svetko VOJIN¹

¹Agricultural Institute of Republic of Srpska, Banja Luka, BiH

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Lakić, Ž., I. Balalić, and S. Vojin (2015): Interpretation of genotype × environment interaction in perennial ryegrass (Lolium perenne L.)- Genetika, Vol 47, No. 2, 509-522.

The aim of this field study was to evaluate genotype × year interaction for spike length, 1000 seed weight, seed number per g and hectoliter mass in perennial ryegrass, using eight divergent genotypes across two growing seasons. Interaction was evaluated using AMMI (Additive Main Effects and Multiplicative Interaction) model. All traits schowed additive (genotype, year) and non additive (genotype × year interaction) variation. On the basis od AMMI1 biplot genotype G4 showed most stability for all examined traits, so it can be recommended for sowing in different environmental conditions. Also, the genotype G4 could be used in the creation of new local varieties of perennial ryegrass.

Key words: AMMI model, interaction, hectoliter mass, perennial ryegrass, spike length, 1000 seed weight

Corresponding author: Željko Lakić, Agricultural Institute of Republic of Srpska, Banja Luka, BiH

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 523- 534

Serbia

UDC 575
DOI: 10.2298/GENSR1502523Y

Original scientific paper

A MICROARRAY GENE EXPRESSIONS WITH CLASSIFICATION USING EXTREME LEARNING MACHINE

M. YASODHA¹ and P. PONMUTHURAMALINGAM²

¹Ph.D Research Scholar, Government Arts College (Autonomous), Coimbatore, Tamilnadu, India

²Government Arts College (Autonomous), Coimbatore, Tamilnadu, India

Abstract

M. Yasodha and Dr. P. Ponmuthuramalingam (2015): A microarray gene expressions with classification using extreme learning machine-Genetika, Vol 47, No. 2, 523-534.

In the present scenario, one of the dangerous disease is cancer. It spreads through blood or lymph to other location of the body, it is a set of cells display uncontrolled growth, attack and destroy nearby tissues, and occasionally metastasis. In cancer diagnosis and molecular biology, a utilized effective tool is DNA microarrays. The dominance of this technique is recognized, so several open doubt arise regarding proper examination of microarray data. In the field of medical sciences, multicategory cancer classification plays very important role. The need for cancer classification has become essential because the number of cancer sufferers is increasing. In this research work, to overcome problems of multicategory cancer classification an improved Extreme Learning Machine (ELM) classifier is used. It rectify problems faced by iterative learning methods such as local minima, improper learning rate and over fitting and the training completes with high speed.

Key words: Gene Expression Data, Gene Ranking, Feature Selection and Classification

Corresponding author: M. Yasodha, Ph.D Research Scholar, Government Arts College (Autonomous), Coimbatore, Tamilnadu, India. E-Mail: yasodhamii@gmail.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 535- 548

Serbia

UDC 575

DOI: 10.2298/GENSR1502535B

Original scientific paper

STUDIES ON FLUCTUATING ASYMMETRY (FA) FOR CERTAIN MORPHOLOGICAL TRAITS IN FOUR SPECIES OF THE Drosophila bipectinata COMPLEX

Parul BANERJEE and Bashisth Narayan SINGH^*

Genetics Laboratory, Department of Zoology, Banaras Hindu University Varanasi 221005, India

Abstract

Banerjee P. and B. Narayan Singh (2015): Studies on fluctuating asymmetry (fa) for certain morphological traits in four species of the Drosophila bipectinata complex-Genetika, Vol 47, No. 2, 535-548.

Fluctuating asymmetry (FA) is defined as subtle deviations from perfect bilateral symmetry, evident in differences between the right and the left sides of any given trait. It is a pattern of variation between sides and measures developmental instability. Differences in the level of FA may be used for comparing developmental precision among closely related species and thus may give an idea whether developmental stability was affected during the divergence and separation of populations into distinct species. Keeping this in view, FA was studied in four species of the Drosophila bipectinata complex i.e. D. bipectinata, D. parabipectinata, D. malerkotliana and D. pseudoananassae. In females of all the four species, FA values did not vary significantly for any of the traits considered. However, in case of males, they varied significantly for Wing length (WL) and sex comb tooth number (SCTN). Also, while in females Composite fluctuating asymmetry (CFA) did not exhibit significant variation, in males it was found to vary significantly across the four species. However, Bonferroni t- tests did not reveal any consistent difference in FA levels between any two species. The magnitude of FA was found to differ significantly among traits and CFA values were found to be higher for males than females in all the four species. Therefore, it may be concluded that the level of FA shows trait specific variations and males are more prone to developmental perturbations. However, the FA levels are more or less similar in all the four species of this complex. Thus, developmental precision remains nearly same in all the four species of this complex irrespective of the degree of evolutionary divergence reached.

Corresponding author: Bashisth Narayan Singh, Genetics Laboratory, Department of Zoology, Banaras Hindu University Varanasi 221005, India; Email: bashisthsingh2004@rediffmail.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 549-558

Serbia

UDC 575:633
DOI: 10.2298/GENSR1502549G
Original scientific paper

DETERMINATION OF THE BEST INDIRECT SELECTION CRITERIA IN IRANIAN DURUM WHEAT (Triticum aestivum L.) GENOTYPES UNDER IRRIGATED AND DROUGHT STRESS CONDITIONS

Ahmad Reza GOLPARVAR^1*, Mohammad Mehdi GHEISARI², Davoud NADERI³,

Ali Mehras MEHRABI⁴, Amin HADIPANAH⁵, Saeed SALEHI⁶

¹Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

²Department of Chemistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan ³Department of Horticulture, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

⁴Department of Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

⁵Department of Horticultural, Science and Research Branch, Islamic Azad University, Tehran, Iran

⁶Department of Agronomy, College of Agriculture, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Abstract

Golparvar A. R-, M. M. Gheisari, D. Naderi, A. M. Mehrabi, A. Hadipanah, S. Salehi (2015): Determination of the best indirect selection criteria in Iranian durum wheat (Triticum aestivum L.) genotypes under irrigated and drought stress conditions.-Genetika, Vol 47, No. 2, 549-558.

In order to evaluate and classify morphological and morpho-physiological traits of durum wheat genotypes in drought and irrigated conditions 200 durum wheat genotypes were sown in modify augmented design with four replications during 2013-2014 farming season. Two replications were considered as drought condition and two as irrigated. Factor analysis based on principal component analysis method and varimax rotation indicated that four important factors accounted for about 87 and 92 percent of the total variation among traits in drought and irrigated conditions, respectively. In drought stress condition, the first factor assigned 37 percent of total variation between traits and was significantly related with spike yield and it’s components. Therefore, this factor was regarded as spike seed yield factor. Other factors in drought stress condition accounted for 22, 16 and 12 percent of variation between traits and were entitled as plant seed yield, spike density and assimilate transmission factors, respectively. In irrigated condition, the first factor assigned 36 percent of total variation between traits and was significantly related with traits plant and spike seed yield, plant and spike harvest index, No. seed/spike and plant height. This factor was regarded as plant seed yield factor. Other factors in irrigated condition accounted for 24, 18 and 14 percent of variation between traits and were entitled as production, assimilate transmission and spike density factors, respectively. Overall, results revealed effect of different environments on extracted factors, percent of variation accounted for by factors and traits related with each factor.

Key words: Durum wheat, simple correlation, factor analysis, varimax rotation, drought stress, yield

Corresponding author: Ahmad Reza Golparvar,Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran, dragolparvar@gmail.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 559-570

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:633
DOI: 10.2298/GENSR1502559A

Original scientific paper

COMBINING ABILITY AND HERITABILITY OF SELECTED RICE VARIETIES FOR GRAIN YIELD, ITS COMPONENTS AND GRAIN QUALITY CHARACTERS

Mehrzad ALLAHGHOLIPOUR ^1*, Ezatollah FARSHDFAR², Babak RABIEI³

1* Corresponding author, Department of Plant Breeding, Rice Research Institute of Iran (RRII, Rasht, Iran

2 Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Razi, Kermanshah, Iran

3 Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

Abstract

Allahgholipour M., E. Farshdfar, B. Rabiei(2015): Combining ability and heritability of selected rice varieties for grain yield, its components and grain quality characters-Genetika, Vol 47, No. 2, 559-570.

This study was conducted to determine the combining ability and heritability of rice grain yield, its components and some grain quality traits such as amylose content (AC), gelatinization temperature (GT), gel consistency (GC) and head rice recovery (HRR). The study was commenced by crossing the selected rice varieties based on a full diallel mating design. The F₁ was harvested at the end of the season. In the following season, the crossed, reciprocal and parental lines were planted in randomly complete block design with three replications. Analysis of variance indicated that genotypes were significantly different for all traits. The diallel analysis by Griffing`s method showed highly significant differences for GCA for number of panicles per plant (PN), amylose content, gelatinization temperature and head rice recovery. Highly significant differences were also observed for both SCA and REC for all evaluated characters. The results showed that the grain yield (GY), number of filled grains (FGN), 100-grain weight (HGW) and GC were controlled by non-additive gene action, while the inheritance of PN, AC, GT and HRR were largely controlled by additive gene effects, although non-additive genetic components and reciprocal effect were also involved, which suggest that a selection process could be done in the early generations. The two improved lines (RI18442-1 and RI18430-46) were found to be good general combiners for GY and FGN, while the best combiners for PN was Tarom Mohali and IR50 and for HGW was RI18430-46. The best combinations for GY were RI18430-46 × IR50, Tarom Mohali × RI18447-2 and Daylamani × RI18430-46. The good hybrids were Tarom Mohali × IR50, Line23 × RI18447-2 and Line23 × Backcross line for AC. Narrow sense heritability showed that the GY and GC had the lowest values while the other traits had either moderate or high heritability, which indicates selection in the early generations could be done to fix the favorable genes. In present study, narrow sense heritability was high for AC and moderate for GT, PN and HRR.

Key words: Combining ability, diallel analysis, grain yield, heterosis, narrow sense heritability, rice.

Corresponding author: Mehrzad Allahgholipour, Department of Plant Breeding, Rice Research Institute of Iran (RRII), P.O Box: 1658, Postal Code: 41996-13475, Rasht, Iran,Email address: alahgholipour@yahoo.com Tel: 098-133-6690052 and Fax: 098-133-6690051

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 571-580

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630
DOI: 10.2298/GENSR1502571P

Original scientific paper

ANALYSIS OF INTRA-POPULATION VARIABILITY OF BALD CYPRESS

(Taxodium distichum L. RICH.) IN SEED STAND NEAR BACKA PALANKA

USING RAPD MARKERS

Vladan POPOVIĆ¹⁾, Aleksandar LUČIĆ²⁾, Danijela RISTIĆ²⁾, Ljubinko RAKONJAC¹⁾,

Sabahudin HADROVIĆ³⁾, Snežana MLADENOVIĆ DRINIĆ²⁾

¹Institute of Forestry, Belgrade

²Maize Research Institute, Zemun Polje, Belgrade

³State Enterprise for Forest Management "Srbijašume", Belgrade

Abstract

Popović V., A. Lučić, D. Ristić, Lj. Rakonjac, S. Hadrović, S. Mladenović Drinić (2015): Analysis of intra-population variability of bald cypress (Taxodium distichum L. rich.) in seed stand near Backa palanka using RAPD markers- Genetika, Vol 47, No. 2, 571-580.

The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.

Key words: Bald cypress, RAPD, population, variability, test tree

Corresponding author: Vladan Popović, Institute of foresty, Kneza Višeslava 3, Belgrade, Serbia

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 581-598

Serbia

UDC 575
DOI: 10.2298/GENSR1502581G

Original scientific paper

EVALUATION OF SUNFLOWER GRAIN YIELD COMPONENTS UNDER DIFFERENT LEVELS OF SOIL WATER STRESS IN AZERBAIJAN

Esmaeel GHOLINEZHAD¹, Reza DARVISHZADEH²andIraj BERNOUSI²

¹ Department of Agronomy, Payame Noor University, Tehran, Iran.

² Department Plant Breeding and Biotechnology, Urmia University, Urmia, Iran

Abstract

Gholinezhad E., R. DarvishzadehandI. Bernousi (2015): Evaluation of sunflower grain yield components under different levels of soil water stress in Azerbaijan Genetika, Vol 47, No. 2, 581-598

In order to study yield and yield components of sunflower landraces under water deficit conditions, an experiment was conducted in the experimental field of Agricultural Research Center, West-Azerbaijan in 2012-2013 cropping seasons. The selected genotypes were evaluated in a rectangular 7 × 8 lattice design with two replications. They were treated by three irrigation scenarios including optimum irrigation, moderate stress and severe stress where irrigation was done after depletion of 50%, 70% and 90% of available water, respectively. A number of 56 confectionary sunflower landraces were investigated in this experiment. The results of combined analyzes showed that the single and combined effect of water treatments and genotypes on the majority of traits under study were significant. With increasing the severity of drought stress, grain yield, kernel to grain ratio, number of seeds per head, head diameter, 1000-seed’s weight, biological yield and harvest index decreased while the hollowness percentage increased. Among the studied landraces, the highest grain yield was obtained from 'Angane 4' in optimum irrigation condition whereas in moderate and severe stress, 'Garagoz 1' and 'Salmas-Sadaghian' produced higher grain yield than the other landraces, respectively. At each level of irrigation, genotypes had different responses so that the suitable genotypes could be chosen for different conditions.

Key words: grain yield, genotype sunflower, water treatments

Corresponding author: Esmaeel Gholinezhad, Department of Agronomy, Payame Noor University, Tehran, Iran,E-mail: holinezhad1358@yahoo.com,Phone: +98441,Fax: + 98441

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 599-608

Serbia

UDC 575.630

DOI: 10.2298/GENSR1502599G

Original scientific paper

IMPROVEMENT OF ADVENTITIOUS ORGANOGENESIS FOR REGENERATION OF TRANSGENIC PLANTS IN BLACKBERRY

Alena GAJDOŠOVÁ^1*, Tatjana VUJOVIĆ², Miroslava SÚKENÍKOVÁ¹,Gabriela LIBIAKOVÁ¹

¹Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra 1, Slovak Republic

²Fruit Research Institute, Čačak, Serbia

Abstract

Gajdošová A., T. Vujović, M.Súkeníková,G. Libiaková (2015): Improvement of adventitious organogenesis for regeneration of transgenic plants in blackberry- Genetika, Vol 47, No. 2, 599-608.

The introduction of foreign DNA into the plant genome by Agrobacterium tumefaciens is a promising technique of targeted gene transfer which depends on good working regeneration system. The aim of the work was to elaborate the system for efficient adventitious organogenesis and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of putative transgenic shoots took place from flag explants cultivated vertically on MS medium with 1 mg l^-1 TDZ and 0.02 mg l^-1 IBA followed by transfer on MS medium with 1 mg l^-1 BAP, 0.02 mg l^-1IBA and 0.1 mg l^-1 GA₃ supplemented with 10-15 mg l^-1 hygromycin after transformation by A. tumefaciens strain LBA 4404 carrying plasmid pCambia 1304. Four putative transgenic plants of cv. 'Čačanska Bestrna' were rooted and acclimatized.

Key words: adventitious organogenesis, plant regeneration, Rubus fruticosus L., transgenic

Corresponding author: Alena Gajdošová,Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, SK-950 07 Nitra 1, Slovak Republic, tel: +421 37 6943 315; fax: +421 37 733 66 60; e-mail: alena.gajdosova@savba.sk

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 609-616

Serbia

UDC 575

DOI: 10.2298/GENSR1502609D

Original scientific paper

THE IMPORTANCE OF MTHFR GENE MUTATION DETECTION IN PATIENT WITH RECURRENT MISCARRIAGES

Klaudija DAUGĖLAITĖ¹ and Danielius SERAPINAS^1,2

¹ Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.

²Mykolas Romeris University, Vilnius, Lithuania

Abstract

Daugėlaitė K. and D. Serapinas (2015): The importance of mthfr gene mutation detection in patient with recurrent miscarriages- Genetika, Vol 47, No. 2, 609-616.

Homocysteine is an enzyme encoded by MTHFR (methylenetetrahydrofolate reductase) gene located on chromosome 1. Mutations in MTHFR gene may result in the afflicted metabolism of homocysteine and thus might increase the risk of recurrent miscarriages. In some cases, recurrent pregnancy loss could be prevented by prescribing folic acid and B group vitamin supplements. The demand of MTHFR gene sequencing for variations is commonly overlooked by doctors or genetic counsellors. To highlight this problem we present a case study of recurrent miscarriages in a patient with a homozygous c. 655C>T variation in MTHFR gene. Moreover, we discuss the need of molecular genetic testing for MTHFR gene variations in patients with recurrent miscarriages and the treatment of hyperhomocysteinemia.

Key words: homocysteine, folic acid, . MTHFR, recurrent miscarriage

Corresponding author: Danielius Serapinas, Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences, Eivenių st. 2, Kaunas LT-50009, Lithuania, Email: dserapinas@gmail.com, tel +37061490479 , fax +37037326953

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 617-626

Serbia

UDC 575.633

DOI: 10.2298/GENSR1502617M

Original scientific paper

ADAPTIVE REACTION OF COTTON ACCESSIONS OF G. hirsutum L.

AND G. barbadense L. SPECIES TO DROUGHT STRESS

Afet MAMMADOVA¹, Ramiz ALIYEV¹, Sevda BABAYEVA¹, Mehraj ABBASOV^1,2

¹Genetic Resources Institute of National Academy of Sciences of Azerbaijan, AZ1106, Baku, Azerbaijan

² Baku State University, AZ1048, Azerbaijan

Abstract

Mammadova A., R. Aliyev, S. Babayeva, M. Abbasov (2015): Adaptive reaction of cotton accessions of G. hirsutum L. and G. barbadense L. species to drought stress- Genetika, Vol 47, No. 2, 617-626.

Adaptation, process of the adjustment of a plant to specific environmental conditions, is provided by physiological mechanisms (physiological adaptation), and at population level (species) by mechanisms of genetic variability, heredity and selection (genetic adaptation).Intraspecific and the interspecific physiological assessment of adaptation potential to drought stress was carried out on 267 collection accessions and varieties of a cotton (197 accessions of G. hirsutum L. and 70 of G.barbadense L.). Comparative study of the stress reaction of tested accessions to unfavorable environmental factors was carried out based on stress depression indices of viability of seeds in sucrose solution-imitating drought.As a result of the studies different sensitivity of plants to abiotic stress was determined. The amplitude of the changes of physiological parameter during stress allowed roughly divide cotton accessions within each species into groups by defining different degrees of comparative drought resistance of samples.Analysis of the percentage ratio of high drought resistant accessions within species, revealed differences between G. hirsutum L. and G. barbadense L. For the specie G. hirsutum L. percentage of accessions with high drought resistance was 9.1%, for specie G. barbadense L.-28.6%. G. barbadense L., in our view, is characterized by a greater potential for volatility and acclimatization to the new, unusual conditions, capable of better resisting the adverse effects of drought and has great promise for using in breeding.It was found that because of the stress in drought resistant varieties activation of RNA synthesis, increase of labile and decrease of stable DNA was observed which indicates an increase of the physiological liability and functional activity of the genetic apparatus. In susceptible varieties a reduction in the synthesis of RNA and DNA and displacement of DNA fraction ratio towards the stable were noted.

Key words: cotton, resistance, stress, drought, physiological parameters, nucleic acids

Corresponding author: Mehraj Abbasov, Genetic Resources Institute of National Academy of Sciences of Azerbaijan, Azadliq Ave 155, AZ1106, Baku, Azerbaijan, e-mail: mehraj_genetic@yahoo.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 627-638

Serbia

UDC 575

DOI: 10.2298/GENSR1502627S

Original scientific paper

DETECTION OF TOXIN GENES AND RAPD ANALYSIS OF Bacillus cereus ISOLATES FROM DIFFERENT SOIL TYPES

Dejana SAVIC¹, Dragana JOSIC², Elizabeta RISTANOVIC¹, Radmila PIVIC³,

Aleksandra STANOJKOVIC-SEBIC³, Zorica LEPSANOVIC⁴

¹Institute of Microbiology, Military Medical Academy, Belgrade, Serbia

²Department of Microbiology, Genetic laboratory, Institute of Soil Science, Belgrade, Serbia

³Department for Soil Amelioration and Erosion, Institute of Soil Science, Belgrade, Serbia

⁴Institute of Epidemiology, Military Medical Academy, Belgrade, Serbia

Abstract

Savic D., D. Josic, E. Ristanovic, R. Pivic, A. Stanojkovic-Sebic, Z. Lepsanovic (2015): Detection of toxin genes and RAPD analysis of Bacillus cereus isolates from different soil types-Genetika, Vol 47, No. 2, 627-638.

The aim of this study was to detect genes for enterotoxins (hbla, entFM and bceT) and for emetic toxin (cer), to determine antibiotic resistance, and to estimate intraspecies diversity in B. cereus isolates by RAPD analysis. B. cereus was identified in 12 out of 117 indigenous Bacillus spp. using the classical microbiological methods and PCR. All isolates were resistant to penicillin and ampicillin, two to tetracyclin and four to trimethoprim-sulphamethoxazole. Also, all isolates produced inducible penicillinases and β-lactamase. Toxin genes were detected with PCR. EntFM and cer genes were present in all isolates, hbla in all, but two, and bceT in none. RAPD analysis was performed with four different primers, two of them designed for this study. The intraspecies diversity revealed 10 different patterns at the 90% similarity level. Two separate clusters were formed regardless of a soil type or utilization. The detection of genes encoding toxins in all B. cereus isolates indicated these bacteria as potentially pathogenic and seriously for human health. Regardless of a soil type or utilization, the RAPD analysis showed high intraspecies heterogeneity in B. cereus isolates. To the best of our knowledge, this is the first study to analyse the presence of entero- and emetic toxin genes and genetic heterogeneity in B. cereus isolates from different soil types and different soil utilization in Serbia.

Key words: antibiotic resistance, Bacillus cereus, enterotoxin genes, emetic toxin gene, RAPD, soil types

Corresponding author: Dejana Savic, Department of Microbiology, Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia, Tel (+381) 11 3609404,Fax (+381) 11 2666164,

e-mail dejsav@yubc.net

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp 639-650

Serbia

UDC 575:633.15

DOI: 10.2298/GENSR1502639S

Original scientific paper

Evaluation of drought tolerance in new bulgarian sweet corn genotypes with using stress tolerance indices

Lydia Shtereva, Roumiana Vassilevska-Ivanova*, Boris Kraptchev

Department of Applied Genetics and Biotechnology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria

Abstract

Shtereva L., R. Vassilevska-Ivanova, B. Kraptchev (2015): Evaluation of drought tolerance in new Bulgarian sweet corn genotypes with using stress tolerance indices .- Genetika, Vol 47, No. 2, 639 -650.

Two inbred lines and their heterotic F₁ hybrid of sweet corn (Zea mays L. var. rugosa Bonaf.) were evaluated to assess their reaction for PEG-mediated water stress at the seedling stage in terms of various physiological and biochemical traits. Water potentials were: zero as control and -0.6, -0.9, -1.2 and -1.5 MPa as treatments. Stress-determining parameters such as proline content, malondiadehyde, and hydrogen peroxide were measured. In water stress, the germination was inversely proportional to the Polyethylene glycol concentrations in all three genotypes; roots and shoots length, fresh and dry weight decreased with an increasing of polyethylene glycol concentration. Malondiadehyde, proline, and hydrogen peroxidewere found to be increased at different osmotic gradients in comparison to control. The data observed in the experiments revealed that the line C-6 might be used as a very promising parent to breed and select new drought tolerant sweet corn hybrids. Such a suggestion needs to be further explored by involving a larger set of sweet corn lines and hybrids.

Key words: drought stress, lipid peroxidation, maize, proline, Zea mays L.

Corresponding author: Roumiana Vassilevska-Ivanova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria; Phone: +359 2 9746229; E-mail: ru_vas_bg@yahoo.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 651-663

UDC 575.630

DOI: 10.2298/GENSR1502651M

Original scientific paper

DETECTION AND MOLECULAR CHARACTERIZATION OF Pepper mild mottle virus

IN SERBIA

Dragana MILOŠEVIĆ ¹, Ivana STANKOVIĆ², Aleksandra BULAJIĆ², Maja IGNJATOV¹, Zorica NIKOLIĆ¹, Gordana PETROVIĆ¹, Branka KRSTIĆ ²

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Belgrade, Serbia

Abstract

Milošević D., I. Stanković, A. Bulajić, M. Ignjatov, Z. Nikolić, G. Petrović, B. Krstić (2015): Detection and molecular characterization of pepper mild mottle virus in Serbia.- Genetika, Vol 47, No. 2, 651 -663.

During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P_1,2which do not break the resistance gene L³. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P_1,2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.

Key words: DAS-ELISA, phylogenic analysis, pepper, RT-PCR, sequencing, viruses

Corresponding author: Dragana Milošević, Institute of Field and Vegetable Crops, Makima Gorkog 30, 21000 Novi Sad, Serbia, phone:+381 21 4898155, fax:+381 21421249, E-mail: dragana.milosevic@nsseme.com

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 665-657

Serbia

UDC 575
DOI: 10.2298/GENSR1502665M

Original scientific paper

EVOLUTION OF HYPOTHALAMUS-PITUITARY GROWTH AXIS AMONG FISH, AMPHIBIAN, BIRDS AND MAMMALS

M. MOAEEN-UD-DIN^1þ, G. BILAL¹ and James M. REECY²

¹ Laboratories of Animal Breeding & Genetics, Faculty of Veterinary & Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi (46300, Pakistan

²Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, IA

Abstract

Moaeen-Ud-Din M., G. Bilal and J. M. Reecy (2015): Evolution of hypothalamus-pituitary growth axis among fish, amphibian, birds and mammals- Genetika, Vol 47, No. 2, 665-677.

Hypothalamus-pituitary growth axis (HP growth axis) regulates animal growth and development in pre-natal and post natal life governed by many factors. However, until recently, the evolutionary history of this axis among lineages is not understood. Aim of the present study was to understand the major events in evolution and evolutionary history and trend of HP growth axis. The diversity among Homo sapience, Mus musculus, Rattus norvegicus, Gallus gallus, Danio rerio and Xenopus laevis was determined for genes involved in HP growth axis in current study. Sequences of HP growth axis genes were retrieved from NCBI (http://www.ncbi.nlm.nih.gov/). Nucleotide diversity using Kimura’s two-parameter method; codon-based test of positive selection using the Nei-Gojobori; equality of evolutionary rate with Tajima's relative rate test and phylogenetic history using the RelTime method were estimated in MEGA6. Estimates of the coefficients of evolutionary differentiation based on nucleotides and amino acids substitution patterns of HP growth axis genes showed contrasting evolutionary patterns among the lineages. The results demonstrated that although these genes might have crucial functional roles in each of the species, however, their sequence divergence did not necessarily reflect similar molecular evolution among the species. Codon-based test of positive selection revealed that Human vs Mouse, Chicken vs Rat, Human vs Rat and Mouse vs Rat had similar and higher non synonymous substitutions (P > 0.05). Higher rate of non-synonymous substitutions at similar orthologs level among species indicated a similar positive selection pressure in these species. Results for relative rate test assessed with the chi-squared test showed difference on unique mutations among lineages at synonymous and non synonymous sites except Chicken vs Mouse, Human vs Mouse, Chicken vs Rat, Human vs Rat and Mouse vs Rat. This indicated that the mutagenic process that generates substitutional mutation is taking place at approximately the same rate at synonymous and non-synonymous sites these lineages. Moreover, despite of common ancestry, our results indicate a different divergent time among genes of these species. This is the first demonstration that variable rates of molecular evolution may be present within HP growth axis genes among different species. This difference could be of interest for comparative genomics analysis and physiological genes functions identification among tho comparative genomics, evolution rate, HP growth axis, positive selection se species whose HP growth axis is not explored.

Key words: genetic linkage map, genome length, Lily, map coverage, RIL, SRAP

Corresponding author: Muhammad Moaeen-ud-Din, Lab of Animal Breeding & Genetics,Faculty of Veterinary & Animal Sciences,PMAS-Arid Agriculture University, Rawalpindi (46300), Pakistan, Email: drmoinawan@gmail.com,Ph: 0092-3438702053

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Published in „GENETIKA“ Vol. 47, No. 2(2015), pp. 679-694

Serbia

UDC 575:630

DOI: 10.2298/GENSR1502679Z

Original scientific paper

PERFORMANCE OF Iris variegata GENOTYPES IN DIFFERENT LIGHT CONDITIONS: FLOWERING PHENOLOGY AND REPRODUCTIVE OUTPUT

Uroš ŽIVKOVIĆ, Danijela MILJKOVIĆ, Nataša BARIŠIĆ KLISARIĆ,

Aleksej TARASJEV, Stevan AVRAMOV

Department of Evolutionary Biology, Institute for Biological Research ”Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Abstract

Živković U., D. Miljković, N. Barišić Klisarić, A. Tarasjev, S. Avramov (2015): Performance of Iris variegata genotypes in different light conditions: flowering phenology and reproductive output.- Genetika, Vol 47, No. 2, 679-694.

We analyzed genetic variability and phenotypic plasticity of flowering pattern and reproductive success in 97 clonal genotypes of Iris variegata originating from contrasting light habitats in Deliblato Sands and expressed under different experimental light conditions. Rhizome segments were taken from each of these clones and transplanted in the experimental garden near the Institute for Biological Research in Belgrade. Significant differences between genotypes were found for two traits (start of flowering, average flowering time). Genotypes originating from open and understory habitats significantly differed for three traits (number of flowers, number of capsules/number of flowers, seed mass/capsule). Significant effect of light treatment were found for three traits (number of capsules/number of flowers, seed mass/capsule, average seed mass). Statistically significant correlations between explored traits were generally similar but also to some extent habitat and treatment specific. Comparing these results with research carried out on congeneric species we noted that there are similar responses for some traits, but also significant differences in some components of flowering and fruiting success.

Key words: genetic variability; flowering phenology Iris variegata; light environment;reproductive output

Corresponding author: Stevan Avramov, Department of Evolutionary Biology, Institute for Biological Research ”Siniša Stanković”, University of Belgrade, Belgrade, 11000 Serbia and Montenegro, tel. +381 11 2078376, fax: +381 11 761433; e-mail: stevan@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 47 No. 2(2015), pp. 695-710

Serbia

UDC 575

DOI: 10.2298/GENSR1502695P

Original scientific paper

ASSESSMENT OF SINGLE NUCLEOTIDE POLYMORPHISMS IN SCREENING 52 DNA REPAIR AND CELL CYCLE CONTROL GENES IN FANCONI ANEMIA PATIENTS

Sandra PETROVIC¹, Andreja LESKOVAC¹, Ivana JOKSIC^1,2, Dragana VUJIC^3,
4,

Ana Valenta SOBOT¹, Jelena FILIPOVIC¹ and Gordana JOKSIC¹

¹Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia

²Hospital of Gynecology and Obstetrics “Narodni Front”, Belgrade, Serbia

³Medical School, University of Belgrade, Belgrade, Serbia

⁴Mother and Child Health Care Institute of Serbia “Dr. Vukan Cupic”, Belgrade, Serbia

Abstract

Petrovic S., A. Leskovac, I. Joksic², D. Vujic, A. Valenta Sobot, J. Filipovic and G. Joksic (2015): Assessment of single nucleotide polymorphisms in screening 52 DNA repair and cell cycle control genes in Fanconi anemia patients .- Genetika, Vol 47, No. 2, 695-710.

Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 ‘and 3’ untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.

Key words: DNA repair, Fanconi anemia, single nucleotide polymorphisms

Corresponding author: Sandra Petrovic,Vinca Institute of Nuclear Sciences, University of Belgrade,M. Petrovica Alasa 12-14, 11001 Belgrade, Serbia,Phone: +381 11 340856,Email1: sandra@vinca.rs

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 711-722

Serbia

UDC 575

DOI: 10.2298/GENSR1502711I

Original scientific paper

MOLECULAR AND MORPHOLOGICAL VARIATION IN SOME IRANIAN SAFFRON (Crocus sativus L.) ACCESSIONS

F. IZADPANAH ^a,*, S. KALANTARI ^a, M. E. HASSANI ^b, M. R. NAGHAVI ^c,

M. SHOKRPOUR^a

^a Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj 31587, Iran

^b Faculty of Agriculture, Food and Natural Resources, University of Sydney, Australia

^c Department of plant breeding, Faculty of Agriculture, University of Tehran, Karaj 31587, Iran

Abstract

Izadpanah F., S. Kalantari, M. E. Hassani, M. R. Naghavi , M. Shokrpour (2015): Molecular and morphological variation in some Iranian saffron (Crocus sativus L.) accessions - Genetika, Vol 47, No. 2, 711-722

Saffron (Crocus sativus L.) is renowned as the most expensive spice in the world. This perennial species belongs to Iridaceae family. Germplasm collection and preservation are one of the main priorities for a better and successful crop improvement. In this study, preliminary evaluation of morphological and molecular diversity among 29 accessions of saffron from Iran was investigated. Several important traits such as length and number of leaves, leaves surface, fresh and dry weight of leaves were measured. RAPD markers using 17 primers were also used to evaluate molecular divergence among the accessions. Simple correlation analysis among morphological traits showed significant positive correlations in most characters, but negative correlations between emergence time and other characters. Cluster analyses based on morphological and molecular data produced dissimilar groups in due to data type. In both dendrograms three distinct groups were resulted and the most of the accessions were placed in the first cluster. Also, the groupings showed no association between diversity patterns and geographical origins. In molecular analysis, out of 17 primers that produced 108 polymorphic bands, 12 primers showed complete polymorphism. The maximum and minimum genetic similarities were 0.98 and 0.42, respectively. This results support abilities of these approaches as economical and quick technique to determination of diversity among saffron accessions.

Key words: Crocus sativus L., Germplasm, Morphological and Molecular variation, saffron

Corresponding author: F. Izadpanah, Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj 31587, Iran, Tel.: +98 263 2248 721; fax: +98 263 2248 721.

E-mail addresses: izadapanah@ut.ac.ir, izadpanah_f@yahoo.com

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 723-731

Serbia

UDC 575

DOI: 10.2298/GENSR1502723C

Original scientific paper

HANDEDNESS AND PHENOTYPIC CHARACTERISTICS OF THE HEAD AND FACE

Milena CVETKOVIĆ ¹, and Perica VASILJEVIĆ ²

¹ Elementary School Sreten Mladenović Mika Niš, Serbia

Abstract

Cvetković M., and P. Vasiljević (2015): Handedness and phenotypic characteristics of the head and face- Genetika, Vol 47, No. 2, 723-731.

Craniofacial characteristics are used to identify similarities and differences between human populations or within a single population. The aim of this study is to provide data on the differences/similarities between two groups of subjects, left-handed and right-handed children, based on the parameters that determine the phenotypic characteristics of the head and face: head dimensions, face dimensions, hair color, eye color and earlobe shape. The study participants included 1354 students aged 7 to 15 years from regular schools of southeastern Serbia. The instruments used include: the Edinburgh Handedness Questionnaire for handedness determination, the cephalometer, and the questionnaire. 135 students (9.97%) were identified as left-handed, and the differences in the observed parameters were recorded between left-handed and right-handed girls in relation to the cephalic index, nasal index, head breadth, face breadth, and eye color.

Key words: characteristics of the head, facial characteristics, handedness

Corresponding author: Cvetković Milena, Elementary School Sreten Mladenović Mika, Šabačka 20, Niš, Serbia; phone: +381184232869, fax +381184535300 e-mail: tasan@mts.rs

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 733-739

Serbia

UDC 575

DOI: 10.2298/GENSR1502733G

Original scientific paper

DNA REPAIR GENE XRCC3 241MET VARIANT AND BREAST CANCER SUSCEPTIBILITY OF AZERI POPULATIONIN IRANIAN

Sahar GOHARI-LASAKI¹, Jalal GHARESOURAN¹, Morteza GHOJAZADEH²,

Vahid MONTAZERI³, Hakimeh SAADATIAN¹, Seyyed Mojtaba Mohaddes ARDEBILI^1,3*

¹Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz

²Department RDCC, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

³Department of General Surgery, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

³Hematology and Oncology Research Center, Tabriz,University of Medical Sciences, Tabriz, Iran

Abstract

Gohari-Lasaki S., J. Gharesouran, M. Ghojazadeh, V. Montazeri, H. Saadatian, S. M. M.Ardebili (2015): DNA repair gene xrcc3 241met variant and breast cancer susceptibility of Azeri populationin Iranian- Genetika, Vol 47, No. 2, 733739.

DNA-repair systems are essential for repairing damage that occurs when there is recombination between homologous chromosomes. The gene XRCC3 (X-ray cross complementing group 3) encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage .The Thr241Met (XRCC3-18067C>T, rs861539) substitution, a C to T transition at codon 241 in exon7, thus plays critical roles in cancer development. The aim of this study was association between XRCC3 Thr241Met polymorphism and risk of sporadic breast cancer in Azari population. We analysed DNA samples from 100 sporadic breast cancer patients and 100 healthy women, for XRCC3 Thr241Met polymorphism using PCR-RFLP. Genotype specific risks were tested using chi²-test with 95% confident intervals. Frequency of Thr/Thr at codon 241was 69% in controls and 70% in patients, Thr/Met frequency was 22% in controls and 13 % in patients, the Met/Met genotype was 9% incontrols and 17% in patients. No correlation between the genotype and allele distribution and increased susceptibility for breast Cancer. Our results suggested that in pre-menopausal women, breast cancer riskis not significantly associated with rs861539 in Azari population.

Key words: Breast cancer, Polymorphism, XRCC3

Corresponding author: Seyyed Mojtaba Mohaddes Ardebili, Professor of Human Genetics, Department of Medical Genetics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran,Email:Mohaddesmo@yahoo.com, Cell phone: +989141170345

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 741-750

Serbia

UDC 575.633

DOI: 10.2298/GENSR1502741S

Original scientific paper

ALLELIC STATE AT THE MICROSATELLITE LOCUS XGWM261 MARKING THE DWARFING GENE RHT8 IN EGYPTIAN BREAD WHEAT (Triticum aestivum L.) GENOTYPES RELEASED FROM 1947 TO 2004

^{Khaled
F. M. Salem}

Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

Abstract

Salem F.M. K. (2015): Allelic state at the microsatellite locus XGWM261 marking the dwarfing gene RHT8 in Egyptian bread wheat (Triticum aestivum L.) genotypes released from 1947 to 2004- Genetika, Vol 47, No. 2, 741-608.

Rht8 is widely used in dry environments such as Mediterranean regions where it increases plant adaptability. Variation at the Gatersleben wheat microsatellite Xgwm261 locus, whose 192-bp allele closely linked to the dwarfing gene Rht8, on chromosome 2D within 0.6 cM, was used to screen thirty Egyptian bread wheat genotypes released from (1947-2004) to assess the variation at this locus. There were three microsatellite allelic variants based on size. Screening of this wheat collection showed that the three alleles Xgwm261_-165, Xgwm261_-174 and Xgwm261_-192 bp were the most frequent. The highest allele frequency was observed for a Xgwm261_-165 bp fragment (65.52%) followed by a Xgwm261_-174 bp fragment (24.14%). However, the allele frequency of a Xgwm261_-192bp fragment among these wheat genotypes was 10.34%. The percentage distribution of dwarfing alleles for the microsatellite locus Xgwm261 in the Egyptian wheat breeding programs was 30, 20, 20 and 30% for the wheat breeding program Giza, Sakha, Gemmiza and Sids, respectively. PIC for Xgwm261 was 0.527. Genetic heritage of Egyptian genotypes at the microsatellite locus Xgwm261 is consequence of new parental components usage, carriers short plant and early maturity attributes and consequent selection progeny with these traits in breeding programs. The present study will be helpful in characterization Egyptian wheat genotypes, as well as in accurate selection of parents for wheat breeding program in Egypt.

Key words: Bread wheat (Triticum aestivum L.), Rht8, dwarfing gene, microsatellite marker

Corresponding author: K.Salem, Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, P. O. Box, 79, Egypt,E-mail: salemcairo@yahoo.com

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 751-763

Serbia

UDC 575.633

DOI: 10.2298/GENSR1502751M

Original scientific paper

DRY MATTER ACCUMULATION AND REMOBILIZATION IN WINTER BARLEY

AS AFFECTED BY GENOTYPE AND SOWING DATE

Milan MIROSAVLJEVIĆ^1*, Novo PRŽULJ², Vojislava MOMČILOVIĆ¹, Nikola HRISTOV¹, Ivana MAKSIMOVIĆ³

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia,

² Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzegovina

³Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

Abstract

Mirosavljević M., N. Pržulj, V. Momčilović, N. Hristov, I. Maksimović (2015): Dry matter accumulation and remobilization in winter barley as affected by genotype and sowing date- Genetika, Vol 47, No. 2, 751-763.

Knowledge about the effect of genotypic variation and sowing date on dry matter accumulation, remobilization and partitioning in winter barley is important for crop management. Therefore, in field studies, six winter barley genotypes of various origin and maturity groups were studied across four sowing dates. In general, grain yield and dry matter content decreased with delayed sowing, after mid-October, and average grain yield in late October and November sowing was lower 14.2% and 16.9%, respectively, compared to the yield in the optimal sowing date. Among the tested genotypes, high grain yield and dry matter content was obtained from late and medium early barley genotypes. Delayed sowing dates, on average, reduced dry matter remobilization and contribution of vegetative dry matter to grain yield. In years characterized by high spring precipitation, late September and early October sowing of medium early and late barley genotypes enable increased accumulation and remobilization of dry matter and obtainment of high grain yield.

Key words: Barley (Hordeum vulgare L.); cultivar; sowing date; dry matter; accumulation; remobilization

Corresponding author: Milan Mirosavljević, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: 381214898220; Fax: 381214898227 E-mail: milan.mirosavljevic@nsseme.com

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 765-776

Serbia

UDC 575

DOI: 10.2298/GENSR1502765C

Original scientific paper

ANTHROPOGENETIC VARIABILITY IN THE GROUPS OF HOMO-

AND HETEROSEXUALLY ORIENTED INDIVIDUALS

Suzana CVJETICANIN

Institute of Human Genetics, School of Medicine, University of Belgrade

Abstract

Cvjeticanin S. (2015): Anthropogenetic variability in the groups of homo- and heterosexually oriented individuals- Genetika, Vol 47, No. 2, 765-776.

This population-genetic study compares morphophysiological and genetic variability in a group of homosexually oriented individuals from Serbia (N=96) with control group of heterosexual individuals (N=96) using a test of determination of homozygously recessive characteristics in humans (HRC-test).

Results of our study revealed a statistically significant difference in the mean values of genetic homozygosity (control group 5.0+0.2 ; homosexuals 3.4 +0.1 HRCs, out of 20 observed characteristics) the differences in the distribution type, as well as in the variances of presence of specific combinations of such traits. These results suggest a complex polygenic difference between two observed systems.

There is no difference in the degree of genetic homozygosity between the genders in each tested group of individuals. However, both homosexually oriented females and males have significantly lower mean values of HRCs compared to female and male heterosexuals.

Key words: anthropogenetic variability, genetic homozygosity, homosexuals, homorecessive characteristics

Corresponding author: Suzana Cvjeticanin, Institute of Human Genetics, School of Medicine, University of Belgrade,Visegradska 26, 11000 Belgrade, Serbia,Tel/Fax. +381 11 3607040,Tel +381 60 3534821,E-mail: cujasimsi@gmail.com

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