GENETIKA, Vol. 52, No. 1 (2020)
Anamarija KOREN, Vladimir SIKORA, Biljana KIPROVSKI, Milka BRDAR-JOKANOVIĆ, Milica AĆIMOVIĆ, Bojan KONSTANTINOVIĆ, Dragana LATKOVIĆ
CONTROVERSIAL TAXONOMY OF HEMP [Abstract] [Full text]
Zahra NOORMOHAMMADI, Narges ASGHARI-MOONEGHI, Farah FARAHANI
EFFECT OF GAMMA RADIATION ON MORPHOLOGICAL AND GENETIC VARIATION IN REGENERATED PLANTLETS Catharanthus roseus L. (G) Don [Abstract] [Full text]
Ejaz-ul-HASAN, Farooq Ahmad KHAN, Sajida HABIB, Hafeez Ahmad SADAQAT, Shahzad Maqsood Ahmad BASRA
GENETIC DIVERSITY OF SUNFLOWER GENOTYPES UNDER DROUGHT STRESS BY PRINCIPLE COMPONENT ANALYSIS [Abstract] [Full text]
Tanja
KRMPOT, Ljubomir RADOŠ, Aleš VOKURKA
GENETIC CHARACTERISATION OF AUTOCHTHONOUS SWEET CHERRY
GENOTYPES (Prunus avium L.) USING SSR MARKERS [Abstract] [Full
text]
Mehmet Ramazan BOZHUYUK, Sezai ERCISLI, Rayda Ben AYED, Tunde JURİKOVA, Hafize FIDAN, Gulce ILHAN, Gursel OZKAN, Halil Ibrahim SAGBAS
COMPOSITIONAL DIVERSITY IN FRUITS OF ROWANBERRY (Sorbus aucuparia L.) GENOTYPES ORIGINATING FROM SEEDS [Abstract] [Full text]
Andrea Pirković-Čabarkapa, Lada Živković, Mirjana Zlatković-Švenda, Sunčica Borozan, Dijana Topalović, Dragana Dekanski, Marija BRUić, Vladan Bajić, Marija Radak-Perović, Biljana Spremo-Potparević
Cytogenetic
alterations IN rheumatoid arthritis patients treated with methotrexate AND Dry olive leaf extract [Abstract]
[Full
text]
EVALUATION OF YIELD AND AGRONOMIC TRAITS OF NEW WINTER BREAD WHEAT CULTIVARS [Abstract] [Full text]
Vesna ĐORĐEVIĆ, Zvezdana JEMUOVIĆ, Sandra PEKIĆ, Marina DJUROVIĆ
CYTOGENETIC FINDINGS IN SERBIAN PATIENTS WITH KLINEFELTER SYNDROME [Abstract] [Full text]
Emre SEVİNDİK, Mehmet Yavuz PAKSOY, Melike AYDOĞAN, Feyzanur TOPSEÇER
GENETIC VARIATION AND MOLECULAR RELATIONSHIPS TAXA OF Conringia HEIST. EX FABR. (BRASSICACEAE) BASED ON RAPD MARKERS IN TURK [Abstract] [Full text]
Seda
ORENAY-BOYACIOGLU, Elmas KASAP, Hakan YUCEYAR, and Mehmet KORKMAZ
ASSOCIATION OF INTERLEUKIN 12B RS3212227
POLYMORPHISM WITH GASTRIC CANCER, INTESTINAL METAPLASIA, AND Helicobacter pylori INFECTION [Abstract] [Full
text]
Ivan BUSARČEVIĆ, Svetlana VOJVODIĆ, Una VOJVODIĆ
ASSOCIATION BETWEEN SECRETOR STATUS AND LEWIS PHENOTYPE WITH SERONEGATIVE SPONDYLOARTHRITIS AS INDICATOR OF AUTOIMMUNITY [Abstract] [Full text]
Andrija BRKIĆ, Domagoj ŠIMIĆ, Antun JAMBROVIĆ, Zvonimir ZDUNIĆ, Tatjana LEDENČAN, Emilija RASPUDIĆ, Mirjana BRMEŽ, Josip BRKIĆ, Maja MAZUR, Vlatko GALIĆ
QTL ANALYSIS OF WESTERN CORN ROOTWORM RESISTANCE TRAITS IN MAIZE IBM POPULATION GROWN IN CONTINUOUS MAIZE [Abstract] [Full text]
Olga Sergeevna ANTONOVA, Hadil Mohamed KATHOM, Evgeni GRIGOROV, Rada Georgieva STANEVA, Savina Petrova HADJIDEKOVA, Draga Ivanova TONCHEVA, Daniela Mircheva AVDJIEVA-TZAVELLA
BENEFITS AND CHALLENGES IN CONDUCTING LONG-TERM GROWTH HORMONE THERAPY IN PATIENTS WITH PRADER-WILLI SYNDROME [Abstract] [Full text]
Ali H. ABU-ALMAATY, Iman M. BAHGAT, Zaineb M. AL-TAHR
USING SDS-PAGE AND ISSR AS BIOCHEMICAL MARKERS FOR ASSESSMENT THE GENETIC SIMILARITY AND PROTEIN ANALYSIS OF SOME CYPRINID FISH SPECIES[Abstract] [Full text]
Dragan NIKOLIĆ, Dragan MILATOVIĆ, Aleksandar RADOVIĆ, Jugoslav TRAJKOVIĆ
DISTINGUISHING OBLAČINSKA SOUR CHERRY CLONES (Prunus cerasus L.) BY POLLEN MORPHOLOGY [Abstract] [Full text]
Neda ATAZADEH, Masoud SHEIDAI, Farideh ATTAR , Fahimeh KOOHDAR
GENETIC AND MORPHOLOGICAL DIVERSITY IN Cousinia calocephala JAUB. & SPACH (ASTERACEAE; SECTION CYNAROIDEAE BUNGE) POPULATIONS [Abstract] [Full text]
Bahram DEHDAR, Saleh AMIRI, Bahman PANAHI, Reza MOHAMMADI
COMBINING ABILITY ANALYSIS OF TUBER YIELD AND RELATED TRAITS IN POTATOES [Abstract] [Full text]
Sinem AYDIN
TOTAL PHENOLIC CONTENT, ANTIOXIDANT, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES, FT-IR ANALYSES OF Brassica oleracea L. var. acephala AND Ornithogalum umbellatum L. [Abstract] [Full text]
Primož TITAN, Jernej ISKRA, Koji MURAI, Vladimir MEGLIČ
CHEMICALLY INDUCED MALE STERILITY IN COMMON WHEAT MEDIATED BY AEGILOPS CYTOPLASM [Abstract] [Full text]
Hüsnü AKTAŞ, Okan ŞENER
EFFECT OF HMW AND LMW GLUTENIN ALLELES ON QUALITY TRAITS OF BREAD WHEAT [Abstract] [Full text]
Jelena VANCETOVIC, Marija KOSTADINOVIC, Sofija BOZINOVIC, Ana NIKOLIC, Jelena VUKADINOVIĆ, Ksenija MARKOVIC, Dragana IGNJATOVIC-MICIC
AGRONOMIC, BIOCHEMICAL AND GENETIC ATTRIBUTES OF MAIZE HIGH GRAIN QUALITY ACCESSIONS [Abstract] [Full text]
Abouzar ABOUZARI, Mahmood SOLOUKI, Behrouz GOLEIN, Barat Ali FAKHERI, Ateh SABOURI, Ahmad Reza DADRAS
UNRAVELING THE CRYPTIC GENETIC VARIATION AND POPULATION STRUCTURE OF THE CITRUS GENOTYPES WITH UNKOWN ORIGIN [Abstract] [Full text]
Özge KURD, Ersin DOĞAÇ, Vatan TAŞKIN, Belgin GÖÇMEN TAŞKIN
ISSR MARKER BASED POPULATION GENETIC STUDY OF MEDITERRANEAN FRUIT FLY Ceratitis capitata (Diptera: Tephritidae) [Abstract] [Full text]
Saeed MOHSENZADEH, Masoud SHEIDAI, Fahimeh KOOHDAR
SPECIES RELATIONSHIPS OF GENUS Plantago L. (PLANTAGINACEAE) BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE [Abstract] [Full text]
Osama Zuhair KANBAR, Csaba LANTOS, Erzsebet KISS, Janos PAUK
ANDROGENIC RESPONSES OF WINTER WHEAT (Triticum aestivum L.) COMBINATIONS IN IN VITRO ANTHER CULTURE [Abstract] [Full text]
Snežana GOŠIĆ DONDO, Dragan GRČAK, Milosav GRČAK, Danijela KONDIĆ, Djuradj HAJDER, Željko POPOVIĆ, Desimir KNEŽEVIĆ
THE EFFECT OF INSECTICIDES ON THE TOTAL PERCENTAGE OF Ostrinia nubilalis Hbn ATTACK ON MAIZE HYBRIDS [Abstract] [Full text]
Bojan MITROVIĆ, Bojan DRAŠKOVIĆ, Dušan STANISAVLJEVIĆ, Milica PERIŠIĆ, Petar ČANAK, Ivana MITROVIĆ, Sonja TANČIĆ-ŽIVANOV
ENVIRONMENTAL MODELING OF INTERACTION VARIANCE FOR GRAIN YIELD OF MEDIUM EARLY MATURITY MAIZE HYBRIDS [Abstract] [Full text] [Supplement]
Jakub KUBIŚ, Marek KMIEĆ, Jan BIŃKOWSKI, Marta SRÓŻYŃSKA
THE INFLUENCE OF HIGH AND LOW LEVELS OF ARSENIC IN THE HUMAN BODY ON THE PROCESS OF CARCINOGENESIS, BASED ON FUNCTION, EXPRESSION AND GENETIC POLYMORPHISMS IN THE GENES ENCODING THE TWO ARSENIC TRANSPORTERS - WATER CHANNELS AQP AND GLUCOSE TRANSPORTERS GLUT (SLC2A) [Abstract] [Full text]
M. M. I. RASHAD and Umakanta SARKER
GENETIC VARIATIONS IN YIELD AND YIELD CONTRIBUTING TRAITS OF GREEN AMARANTH [Abstract] [Full text]
Corrigendum: Stefanović M., N. Karaiskou, N. Veličković, M. Kovačević, Z. Ristić, M. Djan (2019): Spatial genetic analysis of roe deer from the northern Serbian province of Vojvodina.- Genetika, Vol 51, No.3, 1127-1138. https://doi.org/10.2298/GENSR1903127S [Corrigendum] [Corrigendum Full text]
Published in „GENETIKA“ Vol. 52, No.1 (2020), pp. 1-13
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.633
https://doi.org/10.2298/GENSR2001001K
Review article
CONTROVERSIAL TAXONOMY OF HEMP
Anamarija KOREN1, Vladimir SIKORA1, Biljana KIPROVSKI1, Milka BRDAR-JOKANOVIĆ1,
Milica AĆIMOVIĆ1, Bojan KONSTANTINOVIĆ2*, Dragana LATKOVIĆ2
1 Institute of Field and Vegetable Crops, Novi Sad, Serbia
2 University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
Abstract
Koren A., V. Sikora, B. Kiprovski, M. Brdar-Jokanović, M. Aćimović, B. Konstantinović, D. Latković (2020). Controversial taxonomy of hemp. - Genetika, Vol 52, No.1, 1-13.
Hemp (Cannabis sativa L.) was one of the earliest domesticated plant species. Biological classification (taxonomy or systematization) manifests evolutional relationships between taxons according to trait similarities. When it comes to taxonomy, hemp is one of the most controversial plant species due to significant effects of environmental conditions on hemp phenology and expression of quantitative traits as well as different levels of gender expression observed in hemp plants. Controversial taxonomy of hemp has gone through several phases throughout history. The attitude on the number of species within the genus Cannabis and the criteria used in taxonomic units division were under dispute. Initially focused on morphological characteristics and geographical origin, the approach was greatly amended by the development of molecular and biochemical techniques. The main cause of taxonomic uncertainties is the inbreeding ability of all wild Cannabis populations, resulting in continual variability of quantitative traits. The aim of the paper is to review the history of Cannabis classification including different approaches to this scientific issue.
Keywords: cannabinoids, Cannabis taxonomy, genus evaluation, hemp
Corresponding author: Bojan Konstantinović, University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia; E-mail: bojank@polj.uns.ac.rs. Phone:+38163518618
Published in „GENETIKA“ Vol. 52, No. 1 (2020), pp.15-28
© 2020Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001015N
Original scientific paper
EFFECT OF GAMMA RADIATION ON MORPHOLOGICAL AND GENETIC VARIATION IN REGENERATED PLANTLETS Catharanthus roseus L. (G) Don
Zahra NOORMOHAMMADI1*, Narges ASGHARI-MOONEGHI1, Farah FARAHANI2
1Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
Abstract
NoormohammadI Z., N. Asghari-Mooneghi, F. Farahani (2020). Effect of gamma radiation on morphological and genetic variation in regenerated plantlets Catharanthus roseus L. (G) Don.- Genetika, Vol 52, No.1, 15-28.
Catharanthus roseus L. (G) Don, commonly known as Madagascar periwinkle, is an important medicinal plant receiving much attention from researchers. This plant is a rich source of terpenoid indole alkaloids (TIAs). The gamma radiation and somaclonal variation may provide suitable methods for genetic changes to increase medicinal components of C. roseus. In the present study, 30 and 60 (Gy) gamma rays were irradiated on seeds. C. roseus seeds were cultured in Murashige and Skoog (MS) medium in three different groups, 0 Gy (control) 30, and 60 Gy gamma irradiations. Morphological characteristics of regenerated plantlets were measured for surface area of leaves, stem length, root length, and fresh weight. The genetic variations were evaluated using nine inter-simple sequence repeats and 25 sequences related amplified polymorphism markers. Morphological results showed a significant difference between studied groups in leaf surface and twisted leaf in 60 Gy irradiated plantlets. The analysis of molecular variance revealed the significant genetic difference among groups. STRUCTURE analysis showed genetic variability both within and among groups. Using the NJ tree analysis, the plants studied were categorized into three distinct groups, which were supported by principal coordinate analysis (PCoA). In conclusion, gamma irradiation produced morphological and genetic changes in C. roseus regenerated in vitro plantlets providing to be a suitable method for further mutation breeding in periwinkle.
Keywords: Gamma ray, Madagascar periwinkle, somaclonal variation, ISSR, SRAP.
Corresponding author: Zahra Noormohammadi, Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran. Tel/Fax: +98 21 44865939.
E-mail: marjannm@yahoo.com, z-nouri@srbiau.ac.ir.
Published in „GENETIKA“ Vol. 52, No. 1 (2020), pp.29-41
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001029H
Original scientific paper
GENETIC DIVERSITY OF SUNFLOWER GENOTYPES UNDER DROUGHT STRESS BY PRINCIPLE COMPONENT ANALYSIS
Ejaz-ul-HASAN1, Farooq Ahmad KHAN1, Sajida HABIB2, Hafeez Ahmad SADAQAT1, Shahzad Maqsood Ahmad BASRA3
1Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan
2Directorate of Oilseeds Research Institute, Faisalabad, Pakistan
3Department of Agronomy, University of Agriculture, Faisalabad, Pakistan
Abstract
Hasan, E.U., F. A. Khan, S. Habib, H. A. Sadaqat, S. M A. Basra (2020). Genetic diversity of sunflower genotypes under drought stress by principle component analysis.- Genetika, Vol 52, No.1, 29-41.
Seventy diverse genotypes were evaluated for drought stress for morphological (shoot and root length, fresh and dry shoot weight, fresh and dry root weight) and physiological traits (stomatal conductance, photosynthesis and transpiration rate) to increase the area under arid and semi-arid climatic condition to overcome the deficiency of edible oil. All genotypes varied significantly for all evaluated traits. High variability for photosynthesis rate and lower for transpiration rate but highly significant. Genotypic variance and its coefficient are almost higher for all characters then environmental variance and its coefficient. Additive gene action was found for stomatal conductance and fresh root weight, good characters for selection having high heritability with increased genetic advance used to find out drought tolerating genotypes. Genotypes ORI 29 and ORI 30 performed better in normal and drought stress condition can be used further breeding programme as a drought tolerant material. In PCA, genotypes ORI 30, ORI 27, ORI 38 and ORI 105 expressed maximum diversity can be used in further hybrid programme.
Keywords: Sunflower, drought stress, morphological traits, PCA, genetic variability
Corresponding author: Ejaz-ul-Hasan, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan, E-mail: HASAN115@umn.edu, Phone # +1 646-549-7988
Published in „GENETIKA“ Vol. 52, No1(2020), pp. 43-53
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001043K
Original scientific paper
GENETIC CHARACTERISATION OF AUTOCHTHONOUS SWEET CHERRY GENOTYPES (Prunus avium L.) USING SSR MARKERS
Tanja KRMPOT1, Ljubomir RADOŠ1, Aleš VOKURKA2
1University of Banja Luka, Faculty of Agriculture, Republic of Srpska, Bosnia and Herzegovina
2 University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
Abstract
Krmpot T., Lj. Radoš, A. Vokurka (2020). Genetic characterisation of autochthonous sweet cherry genotypes (Prunus avium L.) using SSR markers.- Genetika, Vol 52, No.1, 43-53.
The autochthonous local genotypes of sweet cherries, which represent a very important genetic potential for future breeding programs. Understanding of the molecular basis biodiversity is one of the most important factors for the proper conservation, management and use of plant genetic resources.
This paper investigate the genetic variability of 14 genotypes of sweet cherries using 26 SSR markers. The study included eight autochthonous genotypes of sweet cherry taken from four different location and six virus-free reference varieties of sweet cherry. The average genetic distance between them was 0.43. The number of alleles per locus ranged from two to eight. The minimum number of two alleles of polymorphic loci showed EMPa003 and EMPa002, while the highest number of eight loci alleles had PceGA34 and UDP97-402. Results of genetic analysis that were done show that between autochthonous genotypes of sweet cherries ‘Biljur-Bjelica’, ‘Barevka’ and ‘Ašlamka’ (Kriškovci) there was no difference, it is to have the same genetic profil, which indicates that is the synonym, it is the same identity group. Also the autochthonous genotype ‘Crveni Hrušt’ and ‘Nordwunder’ cultivar had the same SSR profile on microsatellite loci tested. The remaining autochthonous genotypes of sweet cherry had a unique genetic profile.
Keywords: Prunus avium L., autochthonous genotype, varieties, genetic diversity, SSR
Corresponding author: Tanja Krmpot, University of Banja Luka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, Republic of Srpska, Bosnia and Herzegovina, E-mail:tanja.krmpot@agro.unibl.org, tel.+38751330941
Published in „GENETIKA“ Vol. 52, No.1 (2020), pp. 55-65
© 2020 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001055B
Original scientific paper
COMPOSITIONAL DIVERSITY IN FRUITS OF ROWANBERRY (Sorbus aucuparia L.) GENOTYPES ORIGINATING FROM SEEDS
Mehmet Ramazan BOZHUYUK1*, Sezai ERCISLI2, Rayda Ben AYED3, Tunde JURİKOVA4, Hafize FIDAN5, Gulce ILHAN2, Gursel OZKAN2, Halil Ibrahim SAGBAS2
1Igdir University, Faculty of Agriculture, Horticulture Department, Igdir, Turkey
2Ataturk University, Faculty of Agriculture, Horticulture Department, Erzurum, Turkey
3University of Sfax, Centre of Biotechnology of Sfax, Tunisia
4Institute for Education of Pedagogics, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, SK-949 74 Nitra, Slovak Republic
5Department of Catering and Tourism, University of Food Technologies, Plovdiv, Bulgaria
Abstract
Bozhuyuk M. R., S. Ercıslı, R. B. Ayed, T. Jurikova, H. Fıdan, G. Ilhan, H. I. Sagbas (2020). Compositional diversity in fruits of rowanberry (Sorbus aucuparia L.) genotypes originating from seeds.- Genetika, Vol 52, No.1, 55-65.
Turkey has great ecological, topological and geographical diversity within the country and this diversity has contributed not only to a high genetic diversity, but has also allowed the successful introduction and cultivation of a great number of fruit tree taxa. Turkey is also known to have a great richness of wild edible fruits with regard to variety and biological diversity. Rowanberry or mountain ash (Sorbus aucuparia L.) is one of the wild edible fruits naturally found most parts of Turkey. Present study describes morphological (tree growth habit, fruit color) and biochemical fruit traits (vitamin C, organic acids, specific sugars, total phenolic content, total anthocyanin content, total antioxidant capacity) of 17 seed propagated rowanberry genotypes. We found significant differences among almost all studied parameters. The genotypes had diverse L, chroma and hue values, which found between 28.76-42.38%; 24.11-29.45% and 33.13-42.66%, respectively. Among sugars and organic acids, Glucose and Malic acid were dominant in rowanberry fruits, respectively. Total phenolic content, total anthocyanin content, vitamin C and antioxidant activity varied from 123-189 mg GAE per 100 g, 18-57 mg per 100 g, 25.6-40.2 mg per 100 g and 3.36-6.92 mM trolox equivalent per 100 g of fresh weight (FW) basis. Results suggest using rowanberry fruits in production of functional foods with high biologically active properties.
Keywords: Sorbus aucuparia L., seed propagation, functional foods.
Corresponding author: Mehmet Ramazan Bozhuyuk, Igdir University, Faculty of Agriculture, Horticulture Department, Igdir, Turkey. E-mail: mrbozhuyuk@gmail.com
Published in „GENETIKA“ Vol. 52, No. 1 (2020), pp. 67-80
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001067P
Original scientific paper
Andrea Pirković-Čabarkapa1,*, Lada Živković1, Mirjana Zlatković-Švenda2, Sunčica Borozan3, Dijana Topalović1, Dragana Dekanski4, Marija BRUić1, Vladan Bajić5, Marija Radak-Perović2, Biljana Spremo-Potparević1
1Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
2Clinical rheumatology, Institute of Rheumatology, University of Belgrade School of Medicine, Belgrade, Serbia.
3Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Belgrade, Serbia
4Biomedical Research, R&D Institute, Galenika a.d., Belgrade, Serbia.
5Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research “Vinca”, University of Belgrade, Belgrade, Serbia.
Abstract
Pirković-Čabarkapa A., L. Živković, M. Zlatković-Švenda, S. Borozan, D. Topalović, D. Dekanski, M. Bruić, V. Bajić, M. Radak-Perović, B. Spremo-Potparević (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extract.- Genetika, Vol 52, No.1, 67-80.
Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of
Corresponding author: Andrea Pirković Čabarkapa, Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia; E-mail:andrea.pirkovic@pharmacy.bg.ac.rs; Phone +381113951246.
Published in „GENETIKA“ Vol. 52, No.1(2020), pp.81-96
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2001081A
Original scientific paper
EVALUATION OF YIELD AND AGRONOMIC TRAITS OF NEW WINTER BREAD WHEAT CULTIVARS
Bekir AKTAS
Ministry of Agriculture and Forestry, Variety Registration and Seed Certification Center, Ankara, Turkey
Abstract
Aktas B. (2020). Evaluation of yield and agronomic traits of new winter bread wheat cultivars.- Genetika, Vol 52, No.1, 81-96.
Yield and quality attributes of 13 bread wheat cultivars registered for Thrace region in 2018 and 5 standard bread wheat cultivars were determined and compared in this study. Tested cultivars are originated from Turkey, Bulgaria, Romania, Croatia, Austria and Ukraine. Experiments were conducted in 2015-2016 and 2016-2017 growing seasons. Since the locations were not equally distributed to growing seasons, statistical analyses for yield and other parameters were performed through assuming each location as an environment. Therefore, assessments were made over 7 environments. Environment (E) explained 54.9% of the total variation (E+G+GEI), genotype (G) and genotype × environment interaction (GEI) generated 25.6% and 19.5%, respectively. GGE (Genotype + Genotype by environment interaction) biplot analysis was able to explain 78.51% of variation in grain yield. Trial environments gathered under 3 mega-environments. Of the cultivars registered in 2018, Adali, Bc Anica, Topkapi, FDL Miranda, Otilia, ZT Ziyade, Viktoria and Dragana had values larger than the average and thus they had the largest PC1 (Principal component 1) scores. Tested cultivars were also assessed based on vector lengths to average environment coordinate (AEC). Adali cultivar with a high PC1 score and the closest position to AEC apsis was found to be quite prominent for grain yield. In environment-focused assessments, E3, E4 and E5 had the closest position to ideal environment circle. With regard to physical quality attributes, Pehlivan, Bojana and Adali cultivars had high performance values. The cultivar Bojana, Topkapi and Iveta had protein contents above 15%. For Zeleny sedimentation values, Krasunia odes’ka had the best performance.
Keywords: wheat, genotype, environment, variability, stability, grain yield, quality traits.
Published in „GENETIKA“ Vol. 52, No.1 (2020), pp.97-105
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001097D
Original scientific paper
CYTOGENETIC FINDINGS IN SERBIAN PATIENTS WITH KLINEFELTER SYNDROME
Vesna ĐORĐEVIĆ1, Zvezdana JEMUOVIĆ2, Sandra PEKIĆ2,3, Marina DJUROVIĆ2,3
1Clinic of Hematology, Clinical Center of Serbia, Belgrade, Serbia
2Clinic of Endocrinology, Diabetes and Metabolic Disease, Clinical Center of Serbia, Belgrade, Serbia
3Faculty of Medicine, University of Belgrade, Serbia
Abstract
Đorđević V., Z. Jemuović, S. Pekić, M. Djurović (2020). Cytogenetic findings in Serbian patients with Klinefelter syndrome.- Genetika, Vol 52, No.1, 97-105.
Klinefelter syndrome (KS) describes the phenotype of the most common sex chromosome abnormality in humans (1/600 newborn males). The most widespread karyotype in affected patients is 47,XXY, but various others have been described. The aim of this study was to examine the karyotypes of a group of patients suspected of having Klinefelter's syndrome. Between January 1993 and April 2018 104 adult KS patients were evaluated. Cytogenetic analysis was carried out on metaphases obtained from phytohemagglutinin-stimulated peripheral lymphocytes using a standard procedure. Fluorescence in situ hybridization (FISH) analysis was performed on peripheral blood specimens. Vysis CEP X/Y- alpha satellite DNA probes were used to detect X and Y chromosomes. We identified KS presenting the ‘standard’ or 47,XXY karyotype in eighty three (80%) patients, while five (5%) KS patients showed the mosaic karyotype 47,XXY/46,XY and three (3%) patients had the mosaic karyotype 47,XXY/46,XX. In six (6%) cases KS patients with the ‘standard’ karyotype also had autosomal chromosomal abnormalities, while numerical sex chromosome abnormalities, with karyotypes 48,XXYY occurred in two (2%) subjects, 47,XYY in three (3%) and 47,XYY/46,XY in two (2%) individuals. Thus, most of our KS patients had the 'standard', 47,XXY karyotype, but some men formed a group of patients with a diversity of other karyotypes. These disparate chromosomal variants may have different physical and mental implications for the general symptomatology of KS. Therefore, it is important to determine the nature of the karyotype of every male with clinical characteristics of KS in very early childhood in order to initiate an adequate, personalized, medical approach.
Keywords: Klinefelter syndrome, sex chromosome abnormalities
Corresponding author: Vesna Đorđević, PhD, Clinic of Hematology, Clinical Center of Serbia, Belgrade, Serbia, Koste Todorovića 2, Tel: +381 11 366 3382, E-mail: djvesna.kcs@gmail.com
Published in „GENETIKA“ Vol. 52, No. 1 (2020), pp. 107-114
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001107S
Original scientific paper
GENETIC VARIATION AND MOLECULAR RELATIONSHIPS TAXA OF Conringia HEIST. EX FABR. (BRASSICACEAE) BASED ON RAPD MARKERS IN TURKEY
Emre SEVİNDİK1*, Mehmet Yavuz PAKSOY2, Melike AYDOĞAN1, Feyzanur TOPSEÇER1
1 Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
2Munzur University, Tunceli Vocational School,Department of Medical Services and Techniques, Medical Documentation and Secretaryship Pr. Tunceli, Turkey
Abstract
Sevindik E., M. Y. Paksoy, M. Aydoğan, F. Topseçer (2020). Genetic variation and molecular relationships taxa of Conringia Heist. ex Fabr. (Brassicaceae) based on RAPD markers in Turkey.- Genetika, Vol 52, No.1, 107-114.
In this study, genetic variation and phylogenetic analysis of 13 populations of 6 species belonging to Conringia genus spreading in Turkey were performed using RAPD markers. Genomic DNA isolation from the leaves of the Conringia plant samples was performed via using a commercial kit. Seven RAPD primers were used to identify the genetic diversity between the populations. Polymerase Chain Reaction (PCR) was performed using DNA samples and primers. PCR products were resolved using agarose gel electrophoresis and visualized under UV light. All gel images were analyzed, and the absence and presence of polymorphic bands were scored. The total of 34 DNA bands were detected by seven RAPD primers. PAUP 4.0b10 analysis program was used to calculate phylogenetic tree and genetic distances between the species. The phylogenetic tree was obtained using the UPGMA algorithm and it was composed of two clades. According to the PAUP analysis, the species having the closest distance between each other are C. planisiliqua (Ankara-Ayaş) and C. planisiliqua (Ankara-Nallıhan) with the value of 0.000 and those having the longest distance are C. grandiflora (Akseki Çukurköy) and C. orientalis (Elazığ-Baskil) with the value of 0.6000. The results suggest that the RAPD markers are useful tools to demonstrate the genetic relationships between populations of the Conringia species.
Keywords: Conringia, RAPD-PCR, genetic variation, phylogenetic, Turkey
Corresponding author: Emre Sevindik, Department of Agricultural Biotechnology College of Agriculture , Adnan Menderes University ,South Campus, 09970 Cakmar-Aydin- Turkey, Phone: + 90 (256) 772 71 48 ext: 2529, e-mail: ph.d-emre@hotmail.com
Published in „GENETIKA“ Vol. 52, No.1 (2020), pp. 115-126
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001115O
Original scientific paper
ASSOCIATION OF INTERLEUKIN 12B RS3212227 POLYMORPHISM WITH GASTRIC CANCER, INTESTINAL METAPLASIA, AND Helicobacter pylori INFECTION
Seda ORENAY-BOYACIOGLU1, Elmas KASAP2, Hakan YUCEYAR2, and Mehmet KORKMAZ3
1Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey
2Department of Gastroenterology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey
3Department of Medical Biology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey
Abstract
Orenay-Boyacioglu S., Kasap E., Yuceyar H., and M. Korkmaz (2020). Association of interleukin 12B RS3212227 polymorphism with gastric cancer, intestinal metaplasia, and Helicobacter pylori infection. - Genetika, Vol 52, No.1, 115-126.
Interleukin 12 (IL-12) has a key function in promoting Th1 immune response in the gastrointestinal mucosa. Although cytokine gene polymorphisms are associated with increased risk of gastric cancer (GC), studies on different geographic regions and ethnic groups are not able to draw a consistent result. The current case-control study aims to find out an association between a functional IL-12B rs3212227 polymorphism and the susceptibility and clinical features of the study groups, which are GC, Helicobacter pylori–infected and H. pylori–uninfected intestinal metaplasia (IM). In this study, IL-12B rs3212227 polymorphism was genotyped in 35 GC cases, 25 H. pylori–infected IM patients, 25 H. pylori–uninfected IM patients, and 25 control subjects. PCR-RFLP analysis was performed to find out and compare the polymorphism profiles of case biopsies. There was statistical significance in genotype distributions and allelic frequencies in GC patients with proximal arrest in stomach (p=0.042). The rs3212227 genotypes and allelic frequencies were not correlated with any of the study groups (p>0.05). Other clinical features examined in the GC patients were also not correlated with the rs3212227 genotypes and allelic frequencies (p>0.05). Current findings suggest that IL-12B rs3212227 polymorphism may play a role in GC development.
Keywords: Gastric cancer, Helicobacter pylori, intestinal metaplasia, IL-12B gene, rs3212227, polymorphism.
Corresponding author: Seda Orenay-Boyacioglu, Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey, Tel: +90 539 277 7679 Fax: +90 256 2146495, E-mail: sorenay@adu.edu.tr
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 127-136
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001127B
Original
scientific paper
ASSOCIATION BETWEEN SECRETOR STATUS AND LEWIS PHENOTYPE WITH SERONEGATIVE SPONDYLOARTHRITIS AS INDICATOR OF AUTOIMMUNITY
Ivan BUSARČEVIĆ1* Svetlana VOJVODIĆ2, Una VOJVODIĆ3
1Institute for Internal Medicine, Department of gastroenterology and hepatology, Clinical Center of Vojvodina, Novi Sad, Serbia
2Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, University of Novi Sad, Medical Faculty Novi Sad, Novi Sad,Serbia
3Academy of Professional Health Studies, University of Belgrade,Belgrade, Serbia.
Abstract
Busarčević I., S. Vojvodić, U. Vojvodić (2020). Association between secretor status and Lewis phenotype with seronegative spondyloarthritis as indicator of autoimmunity.- Genetika, Vol 52, No.1, 127-136.
The classical paradigm of autoimmune pathogenesis involving specific genetic makeup and exposure to environmental triggers has been challenged recently by the addition of a third element, the loss of intestinal barrier function. Regardless of HLA B27 phenotype or gastrointestinal symptoms, evidence of ileitis, ileocolitis or colitis exists in patients with spondyloarthropathy. The FUT2 secretory gene is a strong candidate for Crohn's susceptibility by shaping the functional states of mucosal microbiota and may thus have influence on the release of zonulin, the main regulator of gut permeability. Gram negative bacteria precipitate and may be involved in the pathogenesis of spondyloarthropathies. Susceptibility to many infectious agents is associated with ABO blood group or secretor state. Patients who cannot secrete ABO and Lewis blood group antigens into body fluids, an ability controlled by a single gene on chromosome 19, are known to be at increased risk of certain autoimmune diseases associated with human leukocyte antigen (HLA) markers. Lewis (Le) blood group phenotype can be used to infer secretor status. The objective of this study was to determine the distribution of secretor state and Lewis blood group phenotype in patients with seronegative spondyloarthropathies and healthy control subjects. Hundred and ten (110) patients with seronegative spondyloarthropathies (58 females and 52 males) and 103 control (74 males and 29 females) subjects participated in this study. Samples of saliva and blood were subjected to haemagglutination inhibition tests for determination of secretor status and Lewis phenotype. A total of 92(84%) patients and 92 (89%) control subjects were secretors while 18 (16%) patients and 11 (11%) control subjects were non-secretors.There was no statistically significant difference (χ2 1,461 p<0,05 and degrees of freedom 1) in distribution of secretor status in comparison to seronegative spondyloarthropathies by comparing two observed populations. Seven patients had modified (reduced) expression of Lewis b antigen on their erythrocytes. Reduction of Lewis b antigen expression was not observed on erythrocytes of healthy subjects. Reduced expression of Lewis b antigen could be a consequence of the inflammatory process within the gut and it also suggests several pathogenic mechanisms which may be relevant to the synthesis of Lewis antigens inside the gut or its absorption on erythrocytes in patients with spondyloarthropathy.
Keywords: Secretor status, Lewis antigen, gastrointestinal tract, autoimmunity, spondyloarthropathy.
Corresponding author: Ivan Busarčević, Institute for Internal Medicine, Department of gastroenterology and hepatology, Clinical Center of Vojvodina, 21000 Novi Sad, Serbia. E-mail address: ivan.busarcevic@gmail.com. Phone: +3810638309622
Published in „GENETIKA“ Vol. 52, No.1(2020), pp. 137-148
© 2020 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2001137B
Original scientific paper
QTL ANALYSIS OF WESTERN CORN ROOTWORM RESISTANCE TRAITS IN MAIZE IBM POPULATION GROWN IN CONTINUOUS MAIZE
Andrija BRKIĆ1*, Domagoj ŠIMIĆ1,2, Antun JAMBROVIĆ1,2, Zvonimir ZDUNIĆ1,2, Tatjana LEDENČAN1, Emilija RASPUDIĆ3, Mirjana BRMEŽ3, Josip BRKIĆ1, Maja MAZUR1, Vlatko GALIĆ1
1Agricultural Institute Osijek, Department for Breeding and Genetics of Maize, Osijek, Croatia
2Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Zagreb, Croatia
3Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Department for Plant Protection, Chair for Entomology and Nematology, Osijek, Croatia
Abstract
Brkić A., D. Šimić, A. Jambrović, Z. Zdunić, T. Ledenčan, E. Raspudić, M. Brmež, J. Brkić, M. Mazur, V. Galić (2020). QTL analysis of Western corn rootworm resistance traits in maize IBM population grown in continuous maize.- Genetika, Vol 52, No.1, 137-148.
The western corn rootworm (Diabrotica virgifera virgifera LeConte; WCR) is the most destructive maize pest in the USA, and one of the most dangerous pests in Europe. In just over a decade from the introduction it became one of the most serious pests in Croatia. Native resistant cultivars could be an efficient strategy for WCR control. Although research in WCR host plant resistance has continuously been conducted for over 70 years, no resistant maize hybrid is commercially available today. The aim of this study was to evaluate resistance traits in the maize IBM intermated recombinant inbred lines (IRILs), and detect QTLs associated with resistance to WCR herbivory. Total of 207 recombinant inbred lines were phenotyped for standard WCR resistance traits. Several associated QTLs were detected on chromosomes 1, 5, 6, 8, 9, and 10. QTLs on chromosomes 1 (6.41 % variance explained), and 6 (9.26 % variance explained) were mapped in regions harboring genes connected with plant defense against herbivory. Detected QTLs provide relevant guidelines for future research on WCR resistance in maize.
Keywords: QTL analysis, western corn rootworm, IBM population, maize
Corresponding author: Andrija Brkić, Agricultural Institute Osijek, Department for Breeding and Genetics of Maize, Južno predgrađe 17, Osijek 31000, Croatia, E-mail: andrija.brkic@poljinos.hr, Telephone: +385 31 515 588
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 149-160
© 2020Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001149A
Original scientific paper
BENEFITS AND CHALLENGES IN CONDUCTING LONG-TERM GROWTH HORMONE THERAPY IN PATIENTS WITH PRADER-WILLI SYNDROME
Olga Sergeevna ANTONOVA1*, Hadil Mohamed KATHOM2, Evgeni GRIGOROV3, Rada Georgieva STANEVA1, Savina Petrova HADJIDEKOVA1, Draga Ivanova TONCHEVA1, Daniela Mircheva AVDJIEVA-TZAVELLA2
1Department of Medical Genetics, Medical Faculty, Medical University of Sofia, Bulgaria
2 Department of Clinical Genetics, University Pediatric Hospital, Medical University of Sofia, Sofia, Bulgaria
3Department Pharmaceutical sciences and Pharmaceutical management, Medical University of Varna, Bulgaria
Abstract
Antonova Sergeevna O., Kathom M. H., Grigorov E., Staneva Georgieva R., Hadjidekova Petrova S., Toncheva Ivanova D., Avdjieva-Tzavella Mircheva D. (2020). Benefits and challenges in conducting long-term growth hormone therapy in patients with Prader-Willi syndrome.- Genetika, Vol 52, No.1, 149-160.
The purpose of this report is to comment the results from long-term growth hormone (GH) treatment of Bulgarian patients suffering from rare genetic disease - Prader-Willi syndrome (PWS) with reference to the age, body composition, complications and genetic etiology. Statistical analysis was performed by ANOVA with post hoc Kruskal-Wallis test and Dunn’s multiple comparison tests. In 90% of the patients maternal uniparental disomy (mUPD) was found to be the cause of the disease. No cases due to imprinting defects are found. The BMI data shows no statistically significant difference between BMI at diagnosis (21.850), at the beginning of the GH therapy (21.852) and current BMI (24.09) - measured under the GH background. Early GH treatment allows to overcome arising obstacles in time and to improve the quality of life for PWS children and their families. During the fourteen years study period only ten patients were diagnosed with the disease. Ninety percent (n=9) of the children were found to be with maternal UPD (mUPD) and only one case was due to deletion in 15q11-13. These results are in agreement with other studies in the field which shows the need for reassessment and new robust statistical analysis of the frequency of genetic mechanisms for PWS.
Keywords: PWS, growth hormone, treatment, mUPD.
Corresponding author: Olga Antonova, Department of Medical Genetics, Medical Faculty, Medical University-Sofia, Bulgaria, 2 “Zdrave” str., 1431 Sofia, E-mail: olga_boyanova@yahoo.com, phone: 00359 884 209.
Published in „GENETIKA“ Vol. 52, No.1(2020), pp. 161-175
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001161A
Original scientific paper
USING SDS-PAGE AND ISSR AS BIOCHEMICAL MARKERS FOR ASSESSMENT THE GENETIC SIMILARITY AND PROTEIN ANALYSIS OF SOME CYPRINID FISH SPECIES
Ali H. ABU-ALMAATY1*, Iman M. BAHGAT1, Zaineb M. AL-TAHR2
1Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt
2Zoology Department, Faculty of Science, Al Jabal Al Gharbi University, Libya
Abstract
Abu-Almaaty A. H., I. M. Bahgat, Z. M. Al-Tahr (2020). Using SDS-page and ISSR as biochemical markers for assessment the genetic similarity and protein analysis of some Cyprinid fish species.- Genetika, Vol 52, No.1, 161-175.
Genetics similarity and protein analysis of some cyprinid fish were studied using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Inter-simple sequence repeated (ISSR Markers). Species Pethia nigrofasciatus, Barbonymus schwanenfeldii, Puntius tetrazone and Brachydanio rerio were collected from the fish farms in Damietta to study the genetic variability among them. Eleven ISSR primers were tested to assess the effectiveness of ISSR analysis in discriminating among the four applied fish species. We observed varied size of amplified products depending upon the sequence of ISSR primers and genotypes used. A total of 131 discrete amplified products were obtained (size 79 to 1185 bp approximately) with polymorphism 95%. Out of 131 products, 63 bands were species specific markers indicating high level polymorphism among species. The highest and lowest number of ISSR bands detected for primers ISSR 15 and ISSR16 was 18 and 17 respectively. 7 bands were most relevant as found monomorphic in all four species of family: Cyprinidae. Highest similarity observed between Pethia nigrofasciatus and Barbonymus schwanenfeldii 80% and lowest similarity was between Pethia nigrofasciatus and Brachydanio rerio 31 %. The protein analysis by SDS-PAGE produced 29 bands of molecular weight ranging from 11 to 132 KD with polymorphism 14%. This study concludes that ISSR-based DNA analysis bands and protein profile in muscles from Pethia nigrofasciatus and Barbonymus schwanenfeldii are the most closest species compared molecularly compared with other species used in this study.
Keywords: Genetics, Cyprinid fish, fingerprint, ISSR, Protein analysis.
Corresponding author: Ali Hussein Abu-Almaaty, PhD., Biotechnology Program, Department of Zoology, Faculty of Science, Port Said University, Port Said, 42521, Egypt. E-mail: ali_zoology_2010@yahoo. Tel: 00201093041699
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 177-186
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001177P
Original scientific paper
ARE THE GENES CA6, TAS2R38, TCF7L2, FTO, TAS1R2, TAS1R3, GNAT3 RECEPTOR POLYMORPHISMS INVOLVED ON EXCEPTIONAL LONGEVITY
AND RISK OF SARCOPENIA? A CROSS SECTIONAL STUDY IN ITALIAN AGING POPULATION
Simone PERNA
Department of Biology, College of Science, University of Bahrain, Sakhir Campus, Kingdom of Bahrain, Zallaq P.O. Box 32038, Kingdom of Bahrain
Abstract
This study hypothesized that genetic polymorphisms of different genes could modulate the lifespan and muscle health or sarcopenia. We investigated the possible associations between longevity and the common genetic variation polymorphism such as rs713598, rs7903146, rs9939609, rs35874116, rs2274333, rs7792845, rs35744813 in a population of 360 Italian elderly aged 65- 100 years. The polymorphism rs9939609, of the FTO gene, shows an association (p< 0.001) with longevity. In particular, the frequency of T/T homozygotes increases gradually from 35% in subjects aged 65-80 ys up to 80% in centenarians. The polymorphism of rs2274333 (CA6) gene shows that A/G frequency increases continuously through older classes reaching the 80% in (95-100) (p trend <0.05). Another important founding regards the polymorphism of rs35744813 of TAS1R3 gene. The C/C genotype frequency is over 80% in all classes (65–95 years) but appears increase in oldest old and reach 100% (95-100 ys). While T allele frequency decrease continuously through older classes reaching the 0% in T/T and 5% in C/T in (95-100) (p-trend <0.05). These data provide suggestive evidence on the possible correlation between longevity and FTO gene (obesity related), CA6 gene (the gustin- bitter taste related) and TAS1R3 (umami taste stimulus related). None associations with sarcopenia were detected.
Keywords: genes, polymorphism, sarcopenia, longevity.
Corresponding author: Simone Perna, Department of Biology, College of Science, University of Bahrain, Sakhir Campus, Kingdom of Bahrain sperna@ sperna@uob.edu.bh ; Tel.: +973 39 37 99 46
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 187-198
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001187
Original scientific paper
DISTINGUISHING OBLAČINSKA SOUR CHERRY CLONES
(Prunus cerasus L.) BY POLLEN MORPHOLOGY
Dragan NIKOLIĆ1*, Dragan MILATOVIĆ1, Aleksandar RADOVIĆ1,
Jugoslav TRAJKOVIĆ2
1University of Belgrade, Faculty of Agriculture, Belgrade, Serbia
2The College of Agriculture and Food Technology, Prokuplje, Serbia
Abstract
Nikolić D., D. Milatović, A. Radović, J. Trajković (2020). Distinguishing Oblačinska sour cherry clones (Prunus cerasus L.) by pollen morphology.- Genetika, Vol 52, No.1, 187-198.
Morphology and ultrastructure of pollen grains were studied in 13 Oblačinska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 μm and 25.16-28.37 μm respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 μm), and the highest in the clone 3 (49.16 μm). The highest colpus and mesocolpium width had the clone 3 (1.73 μm; 14.87 μm) and the lowest had the clone 7 (1.46 μm; 13.78 μm). All studied clones had striate exine ornamentation. Number of ridges per 100 μm2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 μm and from 0.45 to 0.59 μm, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Oblačinska sour cherry clones.
Keywords: Prunus cerasus, clone, pollen grain, exine patern, scanning electron microscopy
Corresponding author: Dragan Nikolić, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: +381 2615 315, fax: +381 2193 659,
e-mail: nikolicd@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp.199-213
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001199A
Original scientific paper
GENETIC AND MORPHOLOGICAL DIVERSITY IN Cousinia calocephala JAUB. & SPACH (ASTERACEAE; SECTION CYNAROIDEAE BUNGE) POPULATIONS
Neda ATAZADEH1 , Masoud SHEIDAI1* , Farideh ATTAR2 , Fahimeh KOOHDAR1
1Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Evin, Tehran, Iran
2Central Herbarium of Tehran University, School of Biology, College of Science, University of Tehran, Tehran, Iran
Abstract
Atazadeh N., M. Sheidai , F. Attar , F. Koohdarć (2020). Genetic and morphological diversity in Cousinia calocephala jaub. & spach (Asteraceae; section Cynaroideae bunge) populations- Genetika, Vol 52, No.1, 199-214.
The genus Cousinia Cass. of the tribe Cardueae with about 700 species is one of the most diverse genera in Central and SW Asia following Senecio and Vernonia. The section Cynaroideae with 89 species is the largest section of the genus. Cousinia calocephala is the only endemic species of the section distributed in 14 provinces of Iran from Alborz to Zagros mountains. In present study 65 plant specimens of 13 geographical populations of C. calocephala were investigated based on the morphological and genetic (ISSR) data. ANOVA test revealed a significant morphological difference among the studied populations. Similarly, AMOVA test yielded a significant genetic difference between the studied populations, suggesting that the studied populations are morphologically and genetically differentiated. AMOVA test revealed that 94% of the total genetic difference was due to inter-populations genetic differences, while 6% was due to within-species genetic variability. The discriminating power of ISSR loci as determined by Gst against Nm analysis, revealed that almost all ISSR loci have an excellent discriminating power. Thus, ISSR markers are efficient in differentiating of the studied C. calocephala populations. The mantel test, revealed a significant positive correlation between genetic and morphological distance and geographical distance of the studied populations. Genetic analysis results revealed that along with genetic drift, low level of gene flow and migration, adaptive loci also helped populations diverge and adapt these populations to their local condition. Thus, we have three different groups which can be considered as three ecotypes for C. calocephala based on the morphological and genetic data.
Keywords: Cousinia calocephala, Cynaroideae, ISSR, Morphometry.
Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Evin, Tehran, Iran. E-mail: msheidai@yahoo.com; Tel/Fax: +98 2129902111
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp.215-228
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001215D
Original scientific paper
COMBINING ABILITY ANALYSIS OF TUBER YIELD AND RELATED TRAITS
IN POTATOES
Bahram DEHDAR1, Saleh AMIRI2*, Bahman PANAHI2, Reza MOHAMMADI2
1Ardebil Agricultural and Natural Resources Center, Agricultural Research, Education and Extension Organization (AREEO), Ardebil, Iran.
2Department of Genomics, Branch for Northwest & West region, Agricultural Biotechnology
Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran
Abstract
Dehdar B., S. Amiri, B. Panahi, R. Mohammadi (2020). Combining ability analysis of tuber yield and related traits in potatoes- Genetika, Vol 52, No.1, 215-228.
In potatoes (Solanum tuberosum L. 2n=4x=48) both the general combining ability (GCA) effects of parents and specific combining ability (SCA) of crosses are important in conditioning traits of economic importance. The objective of this study was to determine the combining abilities for tuber yield and its components on seven high-yielding commercial cultivars of potato and advanced potato clones. Significant differences were found between the parents in the all of traits that are associated with high genetic diversity between parents. GCA for tuber form, length of stolon and number of tuber per plant were high and for stem height, tuber skin appearance and eye dept were low. Heritability estimated from 49 percent in the number of stem to 84 percent in tuber skin appearance. Both heritability and genetic advance as percent of mean estimates were high for tuber yield, tuber form, length of stolon, tuber size, and number of tuber per plant and tuber size uniformity. AS10, AS12, AS14 and UT43 clones was best general combiner for the tuber yield, tuber size uniformity, eye depth, tuber dry matter and length of stolon. The SCA effects were the highest in the cross Savalan×UT43, Luca×AS20, Stbr2×Savalan and Picasso×HS in the traits were considered. Savalan and UT43 parents used in the crosses also have high GCA in studied traits. Finally results showed that Savalan, UT43, AS10, AS12 and AS14 in the studied traits was the high GCA and better than other parents.
Keywords: Potato, GCA, SCA, heritability, genetic advanced.
Corresponding author: Saleh Amiri, Department of Genomics, Branch for Northwest & West region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran. E-mail: saleh.amiriii@gmail.com
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 229-244
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2001229A
Original scientific paper
TOTAL PHENOLIC CONTENT, ANTIOXIDANT, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES, FT-IR ANALYSES OF Brassica
oleracea L. var. acephala AND Ornithogalum umbellatum L.
Sinem AYDIN
Giresun University, Faculty of Arts and Science, Department of Biology, Giresun, Turkey
Abstract
Aydin S. (2020). Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L.- Genetika, Vol 52, No.1, 229-244.
Brassica oleracea var. acephala and Ornithogalum umbellatum are two plant species which belong Brassicaceae and Liliaceae, respectively. Both of them are used as food and medicinally. O. umbellatum is used as anticancer, diuretic anti anti emetic. B. oleracea var. acephala is used for gastritis, gastric and duodenal ulcers and preventing some cancer forms such as stomach cancer, breast cancer and lung cancer. The aim of this research was to search antioxidant, antibacterial and antifungal activities and FT-IR analyses of ethanol extracts of stem and flower parts of B. oleracea var. acephala and O. umbellatum. Extracts were obtained by Soxhlet extraction. Ethanol extracts of B. oleracea var. acephala showed higher antibacterial activity than ethanol extracts of O. umbellatum. The most effective antifungal extract was ethanol extract of flower part of O. umbellatum against the fungi Candida. tropicalis and Candida. parapsilosis. Antioxidant activity of the extracts were also studied. The highest total phenolic and flavonoid contents were found in flower part of B. oleracea var. acephala as 50.7±0.007 µg GAE/mL and stem part of O. umbellatum as 81.37±0.006 µg CE/mL, respectively. BHT which used as standard antioxidant had higher total antioxidant activity than tested ethanol extracts. DPPH activity of the extracts increased in the following order: Flower part of B. oleracea var. acephala> Stem part of O. umbellatum> Flower part of O. umbellatum> Stem part of B. oleracea var. acephala. These results which obtained from this study demonstrate that B. oleracea var. acephala and O. umbellatum species might be utilized as an alternative to synthetic antioxidans and antimicrobials.
Keywords: Plant extract, antimicrobial activity, antioxidant activity.
Corresponding author: Sinem Aydin, Giresun University, Faculty of Arts and Science, Department of Biology, Giresun, Turkey,email : sinem.aydin@giresun.edu.tr
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 245-256
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2001245T
Original scientific paper
CHEMICALLY INDUCED MALE STERILITY IN COMMON WHEAT MEDIATED BY AEGILOPS CYTOPLASM
Primož TITAN1,2, Jernej ISKRA3, Koji MURAI4, Vladimir MEGLIČ5*
1RGA, Research Genetics and Agrochemistry, Ltd, Murska Sobota, Slovenia
2 University of Maribor, Faculty of Agriculture and Life Sciences, Maribor, Slovenia
3University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, Slovenia
4Fukui Prefectural University, Fukui, Japan
5 Agricultural Institute of Slovenia, Ljubljana, Slovenia
Abstract
Titan P., J. Iskra, K. Murai, V. Meglič (2020). Chemically induced male sterility in common wheat mediated by Aegilops cytoplasm Genetika, Vol 52, No.1, 245-256.
Several approaches have been proposed, in the past, for the development of hybrid wheat varieties. Nevertheless, common wheat hybrids today account for less than 1% of the world's wheat production. The reason for the limited success of hybrid wheat varieties is the fact that to date, a simple and efficient system for the production of hybrid seed has not been developed. A two-line system using photoperiod-sensitive cytoplasmic male sterility (PCMS) caused by Aegilops crassa Boiss. cytoplasm seems to be very promising way to produce hybrid wheat seed on large scale. Non-desirable side effects of PCMS system are the unreliable sterility of the male sterile lines under different latitudes. Therefore, we tried to connect male sterility system based on Ae. crassa cytoplasm with chemical induction of male sterility. The presented results reveal that the majority of male sterility genes are conserved in the Ae. crassa cytoplasm and that their effects can be promoted by chemical signals, such as synthetic auxins (e.g., 2,4-Dichlorophenoxyacetic acid). Our research work represents the development of a novel male sterility system which is controlled by chemical signals (e.g., synthetic auxins and their pro-herbicide analogues) and mediated by Ae. crassa cytoplasm.
Keywords: Aegilops cytoplasm, azetidine-3-carboxylic acid, hybrid wheat, male sterility, 2,4-Dichlorophenoxyacetic acid
Corresponding author: Vladimir Meglič, Crop science department, Agricultural institute of Slovenia, Ljubljana, Slovenia, Phone +386 (0)1 280 51 80, E-mail: vladimir.meglic@kis.si
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 257-271
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2001257A
Original scientific paper
EFFECT OF HMW AND LMW GLUTENIN ALLELES ON QUALITY TRAITS
OF BREAD WHEAT
Hüsnü AKTAŞ1*, Okan ŞENER2
1*Mardin Artuklu University, Vocational School of Kızıltepe, Department of Plant and Animal Production, Mardin, Turkey
2Mustafa Kemal University, Faculty of Agriculture, Department of Field Crop, Hatay, Turkey
Abstract
Aktaş H., O. Şener (2020). Effect of HMW and lMW glutenin alleles on quality traits of bread wheat.-Genetika, Vol 52, No.1, 257-271.
This study was performed to investigate impact of HMW and LMW glutenin alleles on quality traits of bread wheat cultivars. Fifteen bread wheat varieties were used for field trails during 2012-13 and 2013-14 under irrigated conditions of Diyarbakır and Mardin locations, Turkey. We investigated the quality of varieties that have same HMW-GS (High molecular weight subunits) and different LMW-GS (Low molecular weight subunits) or vice versa. Results indicated that GluA3-c > d > e, GluB3-g > b’ = i, and GluD3-b ≥ c > a for LMW-GS, and GluB1-7+8 > GluB1-17+18; GluD1-5+10 > GluD1-2+12 for HMW-GS have higher extensograph dough energy, extensibility, resistance, also higher farinograph stability time, gluten index and grain hardness values. The overall evaluation of the results obtained from this study demonstrated that GluA1-1 or 2*, GluB1-7+8 and GluD1-5+10 for HMW glutenin alleles and GluA3-d, GluB3-g and b, GluD3-c for LMW glutenin alleles are associated with a strong gluten structure and accordingly selection of lines harboring these alleles can contribute to the development of genotypes with high quality in wheat breeding programs.
Keywords: HMW, LMW, glutenin, quality, rheology, dough
Corresponding author: Hüsnü Aktaş, Mardin Artuklu University, Vocational School of Kızıltepe, Department of Plant and Animal Production, Mardin, Turkey. E-mail: h_aktas47@hotmail.com
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp.273-290
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2001273V
Original scientific paper
AGRONOMIC, BIOCHEMICAL AND GENETIC ATTRIBUTES OF MAIZE HIGH GRAIN QUALITY ACCESSIONS
Jelena VANCETOVIC, Marija KOSTADINOVIC, Sofija BOZINOVIC, Ana NIKOLIC, Jelena VUKADINOVIĆ, Ksenija MARKOVIC, Dragana IGNJATOVIC-MICIC*
Maize Research Institute Zemun Polje, Belgrade, Serbia
Abstract
Vancetovic J., M. Kostadinovic, S. Bozinovic, A. Nikolic, J. Vukadinović, K. Markovic, D. Ignjatovic-Micic (2020). Agronomic, biochemical and genetic attributes of maize high grain quality accessions.-Genetika, Vol 52, No.1, 273-290.
Nutritional quality of maize is low because maize protein is poor in several essential amino acids. The purpose of this research was to analyze agronomic traits and kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core collection for grain quality and to identify populations for improving protein quality. Standard ZP341 hybrid was superior for half of agronomic traits tested, especially grain yield, which was higher from 24% to six times. Ten accessions had protein content over 14 % and were further analyzed for amino acid composition and kernel characteristics. Additionally, genetic relationships between the accessions were determined by Simple Sequence Repeats (SSRs) analysis with 30 primers. All accessions showed elevated contents of most essential amino acids. Population L492 with 1.87 and 0.68 g 100g-1 dry weight had the highest contents of leucine and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight and dimensions (p<0.05), but superior in most hardness parameters (p<0.05). Pearson correlations revealed lack of negative correlations between biochemical traits, indicating a possibility for concurrent improvement of several amino acids. The best way of improving protein quality of elite materials is through backcrossing and as populations were chosen according to their good general combining ability (with IoDent, Lancaster and BSSS), they could serve for improvement of elite materials of these genetic origins.
Keywords: breeding, kernel biochemistry, kernel hardness, protein quality.
Corresponding author: Dragana Ignjatovic-Micic: Maize Research Institute Zemun Polje, S. Bajica 1, 11185 Belgrade, Serbia,idragana@mrizp.rs; +381648406029
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 291-309
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001291A
Original
scientific paper
UNRAVELING THE CRYPTIC GENETIC VARIATION AND POPULATION STRUCTURE OF THE CITRUS GENOTYPES WITH UNKNOWN ORIGIN
Abouzar ABOUZARI*1, 2, Mahmood SOLOUKI2, Behrouz GOLEIN3, Barat Ali FAKHERI2, Atefeh SABOURI4, Ahmad Reza DADRAS5
1Agricultural Research and Education Center, AREEO, Mazandaran, Iran
2 Laboratory of Computational Biotechnology and Bioinformatics (CBB), Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, University of Zabol, Zabol, Iran
3 Citrus and Subtropical Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Ramsar, Iran
4 Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
5Olive Research Station of Tarom, Crop and Horticultural Science Research Department, Zanjan Agricultural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Zanjan, Iran
Abstract
Abouzari A., M. Solouki, B. Golein, B. A. Fakheri, A. Sabouri, A. R. Dadras (2020). Unraveling the cryptic genetic variation and population structure of the citrus genotypes with unknown origin - Genetika, Vol 52, No.1, 291-309.
The high diversity of Citrus genotypes can increase the probability of identifying new genetic makeups and even desirable traits. Hence, 76 genotypes with unknown origin and 34 known cultivars from several Citrus species were analyzed by SSR and AFLP markers. Overall, 326 alleles and 545 polymorphic bands were respectively scored for 28 SSR loci and 25 AFLP primer-enzyme combinations. The results of various analyses accomplished with SSR markers were in agreement with AFLP. Accordingly, the true species of citron, mandarin, and pummelo were nested into separate groups. In the studied germplasm, we succeeded in tracking three cryptic origin genomes (2 in SSR and 1 in AFLP analysis) which were distinct from 3 true species, and, interestingly, they contributed to the genetic composition of some control cultivars. The results suggested that the interspecific hybridization and genetic introgression were the main determinants of the Citrus genotypes genetic composition. Hereupon, the share of citron in 36%, mandarin in 34%, and pummelo in 22% of selected accessions estimated more than 50%. Despite the controversy about the origin of lemon and sour orange, the results of different grouping methods suggested that lemon had more affinity with lime, and the genetic contribution of mandarin in sour orange was stronger than that of pummelo.
Keywords: Citrus, cluster analysis, genetic relationships, population structure.
Corresponding author: Abouzar Abouzari, Agricultural Research and Education Center, AREEO, Mazandaran, Iran; E-mail: A.Abouzari@uoz.ac.ir.
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp 311-322
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001311K
Original scientific paper
ISSR MARKER BASED POPULATION GENETIC STUDY OF MEDITERRANEAN FRUIT FLY Ceratitis capitata (Diptera: Tephritidae)
Özge KURD1, Ersin DOĞAÇ2, Vatan TAŞKIN1, Belgin GÖÇMEN TAŞKIN*1
1Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, Kötekli, Muğla, Turkey
2Department of Medicinal and Aromatic Plants, Köyceğiz Vocational School, Muğla Sıtkı Koçman University, Muğla, Turkey
Abstract
Kurd Ö., E. Doğaç, V. Taşkin, B. Göçmen Taşkin (2020). ISSR marker based population genetic study of Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae).-Genetika, Vol 52, No.1, 311-322.
The Mediterranean fruit fly, Ceratitis capitata, is a serious pest of agricultural resources. Despite its economic importance, the population genetic structure of this species is still poorly investigated at micro-geographical level, especially from eastern Mediterranean basin. Knowledge about the genetic structure of C. capitata populations is a necessary requisite for understanding population history of the species and designing successful regional eradication programs. In the current study, the inter-simple sequences repeat (ISSR) markers were employed to assess the genetic diversity and population structure of seven natural populations of C. capitata that were collected from different regions of Turkey. Low to moderate levels of genetic diversity were observed. The estimated values for gene flow (Nm) and coefficient of genetic differentiation among populations (GST) were 3.07 and 0.14, respectively. The results of Principle Component Analysis (PCoA) and Unweighted Pair Group Arithmetic Mean Analysis (UPGMA) tend to be uniform in whole, the Antalya populations was clearly separated from the rest. Local environmental conditions, such as differences in pest control management strategies, agricultural practices, microclimates and human mediated transportations might be important factors in shaping the genetic structure of this species in Antalya. This paper provides useful data for understanding population genetic structure of C. capitata populations in eastern Mediterranean basin and development of effective regional pest management strategies.
Keywords: Ceratitis capitata, Genetic variability, ISSR, Colonization process, Turkey
Corresponding author: Belgin Göçmen Taşkın, Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, Kötekli, Muğla, Turkey. E-mail: bgtaskin@mu.edu.tr. phone:+90-252-2111789
Published in „GENETIKA“ Vol. 52, No.1(2020), pp. 323-334
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.630
https://doi.org/10.2298/GENSR2001323M
Original scientific paper
SPECIES RELATIONSHIPS OF GENUS Plantago L. (PLANTAGINACEAE) BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE
Saeed MOHSENZADEH, Masoud SHEIDAI*, Fahimeh KOOHDAR
Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Abstract
Mohsenzadeh S., M. Sheidai, F. Koohdar (2020). Species relationships of genus Plantago L. (PLANTAGINACEAE) based on molecular and morphological evidence.-Genetika, Vol 52, No.1, 323-334.
The genus Plantago L. is a cosmopolitan genus with more than 200 species and has the greatest dispersal rate in tropical and subtropical regions. The taxonomy of the genus Plantago is controversial at the section and subgenus levels. Therefore, we attempt to determine the relationships among 20 species of this genus for the first time in Iran using both morphological and molecular data. ISSR marker and morphological characteristics were used to examine relationships among different species and compare the results with different classifications. The molecular study of Plantago species showed that ISSR marker is not a good marker at the section and subgenus levels, but is a good marker at the species level and it can identify different genetic groups. Morphological study showed that according to Rahn's taxonomy, the studied species should be in 4 subgenera, but section Albicans is in need of taxonomic revision.
Keywords: ISSR, morphological analysis, Plantago L., Iran.
Corresponding author: Masoud Sheidai, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran . E-mail: msheidai@yahoo.com; Tel/Fax: +98 2129902111
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 335-350
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2001335K
Original scientific paper
ANDROGENIC RESPONSES OF WINTER WHEAT (Triticum aestivum L.) COMBINATIONS IN
IN VITRO ANTHER CULTURE
Osama Zuhair KANBAR1,2, Csaba LANTOS2, Erzsebet KISS3, Janos PAUK2*
1Doctoral School of Plant Science, Szent Istvan University, Gödöllő, Hungary
2Department of Biotechnology, Cereal Research Non-Profit Ltd., Szeged, Hungary
3Genetics, Microbiology and Biotechnology Institute, Szent Istvan University, Gödöllő, Hungary
Abstract
Kanbar Osama Z., C. Lantos, E. Kiss, J. Pauk (2020). Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture.-Genetika, Vol 52, No.1, 335-350.
The androgenic parameters were investigated by in vitro anther culture (AC) on nine F2-5 breeding combinations of winter wheat (Triticum aestivium L.). Each combination produced embryo-like structures (ELS), green plantlets, albino plantlets, transplanted plantlets and acclimatized plantlets, with respect to the number of anthers in AC. The number of AC-derived ELS was between 11.73 and 52.76 ELS/100 anthers with the mean of 26.22 ELS/100 anthers, out of which the number of regenerated green plantlets varied from 3.20 to 26.40 green plantlets/100 anthers and the mean was 9.76 green plantlets/100 anthers, while the number of transplanted plantlets ranged from 2.16 to 21.77 transplanted plantlets/100 anthers. Furthermore, the number of albinos/100 anthers was mitigated and varied between 0.72 and 6.20 albinos/100 anthers. We also studied the number of green and albino plantlets per 100 ELS. The rate of green plantlets per 100 ELS ranged between 14.81% and 64.01%, with the overall mean 33.59%, while the rate of albinos per 100 ELS ranged from 4.82% to 20.70% with the overall mean 11.93%. In our experiment, the rate of acclimatized plantlets (70.15–91.57%) depended mostly on the combination. This study asserted the importance of AC method in wheat for in vitro production of green plants. Although albinism was found in each combination, it did not hinder the production of green plantlets. The satisfying results were achieved in green plantlets production compared to the previously published data, but further improvement will be needed continuously, experiment by experiment. The generated acclimatized plantlets will be used in the wheat breeding program as doubled haploid (DH) lines.
Keywords: Androgenesis, anther culture, haploids, Triticum aestivum L., wheatKeywords: Androgenesis, anther culture, haploids, Triticum aestivum L., wheat
Corresponding author: Janos Pauk, Department of Biotechnology, Cereal Research Non-Profit Ltd., P.O. Box 391, H-6701 Szeged, Hungary, Phone: +36-62-435-235 ext. 2234, Fax: +36-62-434-163, ORCID: 0000-0002-8909-0529, E-mail: janos.pauk@gabonakutato.hu
Published in „GENETIKA“ Vol. 52, No. 1(2020), pp. 351-365
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2001351G
Original scientific paper
THE EFFECT OF INSECTICIDES ON THE TOTAL PERCENTAGE OF
Ostrinia nubilalis Hbn ATTACK ON MAIZE HYBRIDS
Snežana GOŠIĆ DONDO1, Dragan GRČAK2, Milosav GRČAK2,
Danijela KONDIĆ3, Djuradj HAJDER3, Željko POPOVIĆ4, Desimir KNEŽEVIĆ2*
1Maize Research Institute “Zemun Polje”, Belgrade, Serbia
2University of Priština, Faculty of Agriculture, Kosovska Mitrovica–Lešak, Kosovo and Metohia, Serbia
3University of Banja Luka, Faculty of Agriculture, Banjaluka, Republika Srpska, Bosnia & Herzegovina
4University of Novi Sad, Faculty of Science, Novi Sad, Serbia
Abstract
Gošić Dondo, S., Grčak, D., Grčak, M., Kondić, D., Hajder, Dj., Popović, Ž., Knežević, D. (2020): The effect of insecticides on the total percentage of Ostrinia nubilalis Hbn attack on maize hybrids. -Genetika, Vol 52, No1, 351-365.
The objective of the paper was to determine the effect of insecticides on the development of the European corn borer (Ostinia nubilalis Hbn) and the effect of damage in six maize hybrids of different maturity groups. The research involved the following hybrids: ZP 427, ZP 434, ZP 555, ZP 600, ZP 606 and ZP 666. In the field trial, insecticides were applied in two terms, during the flight of O. nubilalis first generation and during the flight of second O. nubilalis generation. We used Fobos EC insecticide and a combination of Match 050 EC + Nurelle D insecticides, whose active substances are different. The active substance in Fobos EC insecticide is bifenthrin 100 g l-1, in Match 050 EC lufenuron 50 g l-1 and, in Nurelle D insecticide, the active ingredient is a combination of chlorpyrifos 500 g l-1 and cypermethrin 50 g l-1. The damage inflicted on plants was assessed in July and September. Fobos insecticide had the best plant protection effect, after its use, the arithmetic mean of the total attack percentage of all hybrids was approximately 81.62% while in the control group, it was approximately 91.72%.
Keywords: Ostrinia nubilalis, maize, insecticide, resistance, variability
Corresponding author: Desimir Knežević, University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lešak, Kosovo and Metohia, Serbia, Phone +381 64 614 88 82, e-mail: deskoa@ptt.rs
Published in „GENETIKA“ Vol. 52 No. 1(2020), pp. 367-378
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2001367M
Original scientific paper
ENVIRONMENTAL MODELING OF INTERACTION VARIANCE FOR GRAIN YIELD OF MEDIUM EARLY MATURITY MAIZE HYBRIDS
Bojan MITROVIĆ1, Bojan DRAŠKOVIĆ2, Dušan STANISAVLJEVIĆ1, Milica PERIŠIĆ1, Petar ČANAK1, Ivana MITROVIĆ3, Sonja TANČIĆ-ŽIVANOV1
1Institute of field and vegetable crops, Novi Sad, Serbia
2KWS Srbija doo, Bečej, Serbia
3Faculty of technology Novi Sad, Novi Sad, Serbia
Abstract
Mitrović B., B. Drašković, D. Stanisavljević, M. Perišić, P. Čanak, I. Mitrović, S. Tančić-Živanov (2020): Environmental modeling of interaction variance for grain yield of medium early maturity maize hybrids -Genetika, Vol 52, No1, 367-378.
The phenomenon of genotype by environment interaction (GEI) represents permanent interest for breeders, geneticists and biometricians with its practical and theoretical aspects. We investigated GEI for grain yield of medium early maturity maize (Zea mays L.) hybrids from the official variety trial network by the Department of Protection and Recognition of Varieties of the Republic of Serbia that includes experimental maize hybrids on eight sites over two years. Environmental variables explained 77.6% and 60.7% of the GEI variation for two consecutive years, respectively. Factorial regression combined with stepwise procedure revealed the model which includes variables precipitation in July (pr7), minimum temperature in May (mnt5), maximum temperature in May (mxt5) and insolation hours in April (sh4), in 2004, and environmental index (EI) and average temperature in September (mt9) in 2005, to be the most explanatory models in the region of Vojvodina (Serbia) in two consecutive years. These results provide a base for further research in GEI and stability analysis, and are a useful tool in characterizing the sub-regions of maize growing area and extending the existing results to new sites.
Keywords: genotype by environment interaction, climatic variables, stability analysis, multi-environment trials.
Published in „GENETIKA“ Vol. 52, No.1(2020), pp. 379-392
© 2020Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001379K
Review paper
THE INFLUENCE OF HIGH AND LOW LEVELS OF ARSENIC IN THE HUMAN BODY ON THE PROCESS OF CARCINOGENESIS, BASED ON FUNCTION, EXPRESSION AND GENETIC POLYMORPHISMS IN THE GENES ENCODING THE TWO ARSENIC TRANSPORTERS - WATER CHANNELS AQP AND GLUCOSE TRANSPORTERS GLUT (SLC2A)
Jakub KUBIŚ1*, Marek KMIEĆ2, Jan BIŃKOWSKI3, Marta SRÓŻYŃSKA4
1West Pomeranian University of Technology Szczecin, Department of Polymer and Biomaterials Science, Szczecin, Poland
2Pomerania Medical Univeristy in Szczecin,Department of Genetics and Pathology, Szczecin, Poland
3West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Genetics and General Animal Breeding, Szczecin, Poland
4University of Medical Sciences, Department of Dermatology, Poznań, Poland
Abstract
Kubiś J., M. Kmieć, J. Bińkowski, M. Sróżyńska. (2020): The influence of high and low levels of arsenic in the human body on the process of carcinogenesis, based on function, expression and genetic polymorphisms in the genes encoding the two arsenic transporters - water channels AQP and glucose transporters glut (SLC2A)-Genetika, Vol 52, No1, 379-392.
Arsenic is a metallic element commonly found in soil, water and plants, and thereby can easily enter the food chain. When arsenic enters the body through food consumption, it subsequently passes into the cells through water channels (AQP) and glucose transporters (GLUT), where it may exert a various metabolic alterations including genotoxicity, which may finally promote carcinogenesis. However, there are human populations showing a reduced adverse effects of arsenic. This is mainly due to a natural selection caused by a long-term environmental exposure to a large doses of arsenic. Aquaporins AQP3, AQP7, AQP9, AQP10 and glucose transporters SLC2A1 (GLUT1), SLC2A4 (GLUT4) are considered as the candidate genes associated with resistance to arsenic in the carcinogenesis process as they are closely related to the occurrence of a various types of cancers, while their products are associated with arsenic transport.
Keywords: cancer, arsenic, AQP, GLUT, tumor
Corresponding author: Jakub Kubiś, West Pomeranian University of Technology Szczecin, Department of Polymer and Biomaterials Science, Aleja Piastów 45, 70-311, Szczecin, Poland. E-mail: jakub.kubis1996@gmail.com, Phone: +48 695 092 013
Published in „GENETIKA“Vol. 52, No.1(2020), pp. 393-407
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2001393R
Original scientific paper
GENETIC VARIATIONS IN YIELD AND YIELD CONTRIBUTING TRAITS OF GREEN AMARANTH
M. M. I. RASHAD1 and Umakanta SARKER1*
1Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
Abstract
Rashad M. M. I. and U. Sarker (2020): genetic variations in yield and yield contributing traits of green amaranth.-Genetika, Vol 52, No1, 393-407.
The experiment was carried out at the experimental farm of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur during 2015 using twenty promising genotypes (AA21 to AA40) of green amaranth in Randomized Complete Block Design (RCBD) with three replications to study the variability, genetic parameters, association of yield related morphological traits to foliage yield and to find out the direct and indirect effects of components characters towards foliage yield. The significant analysis of variance revealed wide range of variability among the twenty genotypes of green amaranth. Except for stem diameter, foliage yield and all the yield contributing morphological traits had high heritability including high genetic advance in percent of mean, high to moderate genotypic and phenotypic variances and coefficient of variations with minimum differences of PCV and GCA indicated that the traits were governed by additive gene actions and had a great scope of selection for the improvement of green amaranth. Correlation study revealed that total biomass and shoot weight showed significant association with foliage yield per m2. Hence, selection based on total biomass and shoot weight could remarkably improve the foliage yield of green amaranth. However, the path coefficient analysis revealed that shoot weight had the highest positive direct effect (1.19) with significant genotypic correlation on foliage yield. It indicated that direct selection based on shoot weight would be effective for the improvement of foliage yield of green amaranth. Mean performance study revealed that the genotypes, such as AA21, AA22, AA24, AA25, AA27, AA28, AA31, AA36 and AA39 had high foliage yields including most of the yield related morphological traits over the grand mean. These genotype could be selected as high yielding varieties or parental lines for future breeding program.
Keywords: Foliage yield, genotypes, path analysis, green amaranth, variability
Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Postal Code 1706. Phone: +880-1716606098, Email address: umakanta@bsmrau.edu.bd
Published in „GENETIKA“Vol. 52, No.1(2020), pp. 409-410
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
https://doi.org/10.2298/GENSR2001409E
Editorial
The authors of the article:
Stefanović M., N. Karaiskou, N. Veličković, M. Kovačević, Z. Ristić, M. Djan (2019): Spatial genetic analysis of roe deer from the northern Serbian province of Vojvodina.- Genetika, Vol 51, No.3, 1127-1138 have informed the Editorial Office of the errors:
On page 1130 line 19 instead GenBank accession numbers:KY114497-KY114515 should be GenBank accession numbers KY114497 – KY114512.
On page 1133 in Table 2 instead of □ should be letter Φ.
On page 1134 line 37 instead Fu's FST should be Fu's FS
The authors have requested for this
error to be corrected. Therefore, the journal is publishing this Corrigendum.
Link to the corrected article https://doi.org/10.2298/GENSR1903127S