Nikolić Biljana, Milojević Nataša, Janković Ivana, Kolarević Stoimir, Jovanović Predrag, Jagodić Aleksandra, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Antimutagenic potential of camphor, α/β thujone and eucalyptol in the bacterial assay system

Mitić-Ćulafić Dragana, Janković Milena, Ćakić Sanja, Vuković-Gačić Branka and Knežević-Vukčević Jelena

The effect of rat liver enzymes on antimutagenic potential of monoterpenes against t-booh-induced mutagenesis in E.coli

Mitić-Ćulafić Dragana, Žegura Bojana, Knežević-Vukčević Jelena and Filipič Metka

Evalution of antigenotoxic potential of linalool, eucalyptol and myrcene against IQ-and PhiP-induced DNA damage

Patenković Aleksandra, Stamenković-Radak Marina, Nikolić Dragana, Marković Tamara and Anđelković Marko

Antigenotoxic potential of tea infusion of Gentana lutea in wing spot test of Drosophila melanogaster

Topic III

Invited lecture

Milenković Ljiljana

Hedgehog signaling pathway in development and tumor formation

Introductory lectures

Novaković Ivana, Damnjanović T., Sango Violeta, Mladenović O., Maksimović N., Mirković Duško, Antonijević Nebojša, Simić-Ogrizović S., Sternic N. and Luković Ljiljana

Polymorphism C677T in the gene encoding for methylenetetrahydrofolate reductase in different vascular disorders

Oral presentations

Milićević Radomir, Branković Ljiljana, Radulović D., Jugović D., Stanković T., Stamenković H., and Kamenov B.

Cytogenetics-the present, the future and perspective

Todorić-Živanović Biljana, Strnad Milica, Stamatović Dragana, Grujović Jelena,Tanić Nikola, Ilić Vesnaand Magić Zvonko

Types of bcr-abl rearrangements in Serbian population and a level of expression in chronic myeloid leucemia patients

Lakić Nina, Krstić Aleksandra, Guć-Šćekić Marija, Kuzmanović Miloš, Mićić Dragan, Janjić Dragana, Konstantinidis Nadaand Kostić Gordana

Molecular diagnostic of chilodhood all

Strnad Milica, Brajušković Goran, Strelić Nataša, Todorić-Živanović Biljana, Stamatović Dragana and Magić Zvonko

Expression of BCL 2 protein and amplification of C-MYC gene in patients with chronic myeloid leukemia

Ćirković Sanja, Guć-Šćekić Marija, Pašić Srđan, Radivojević Danijelaand Jovanović Ankica

Results of cytogenetic and molecular testing in Serbian patients with Nijmegan breakage syndrome

Šupić Gordana, Kozomara Ružica, Branković-Magić Mirjana, Jović Nebojša and Magić Zvonko

Prognostic significance of tumor suppressor genes hypermethylation in oral squamous cell carcinoma

Đorđević Vesna, Denčić-Fekete Marija, Jovanović Jelica, Drakulić Danijela, Stevanović Milena, Janković Gradimir, Gotić Mirjana

Pattern of trisomy 1Q in hematological malignancies:a single institution experience

Jelenković Ankica

Genetics in pharmacology: an individualized medicine

Posters

Živanović Dušica, Šipetić Sandra,Stamenković-Radak Marina and Milašin Jelena

Family history of Graves-Basedow disease and environmental risk factors in etiology of this disease

Janakova Zorica

Population genetics in preclinical practice: presentation of 18 years work carried aut at genetic counselling offis of the medical practice in Jagodina

Popović-Kuzmanović Dragana, Novaković Ivana, Stojanović Ljudmila, Miković Danijela, Mirković Duško, Luković Ljiljanaand Aksentijević Ivona

Case study of possible familial antiphospholipid syndrome

Jelenković Ankica

Pharmacogenetics, drug interactions and adverse drug reactions

Cvetković Dragana, Cvetković Slobodan, and Novaković Ivana

Candidate gene polymorphisms and susceptibility to abdominal aortic aneurysm

Stojković Ljiljana, Živković Maja, Radak Đorđe, Kolaković Ana, Stančić Olja, Stanković Aleksandra and Alavantić Dragan

The association of IL-6 -174G/C gene polymorphism with carotid atherosclerosis in population of Serbia

Kolaković Ana, Stojković Ljiljana, Radak Đorđe, Đurić Tamara, Radak Sandra, Stanković Aleksandra, Alavantić Draganand Živković Maja

Matrix metaloproteinase-9 gene polymorphism -1562C/T as susceptibility factor for carotid atherosclerotic plaque development

Pejin Ivana, Buzadžić Ivana, Janković-Oreščanin Biljanaand Šuput-Tomaš Ljiljana

Distribution of paraoxonase 1 coding region polymorphisms in Serbian population

Đorđević Valentina, Nestorović Aleksandra, Tomić Branko, Miljić Predrag, Kovač Mirjana, Miković Danijela, Antonijević Nebojša, Rakićević Ljiljana and Radojković Dragica

The prevalence of FII A19911G and FII C20221T gene variants in thrombophilic Serbian population

Ilić Vesna, Ilić Miroljub and Magić Zvonko

Determination of ACE gene polymorphism distribution in diabetes nephropathy development

Đan Igor, Đan Mihajla, Obreht Dragana, Jovanović Nataša, Stokić Edita,Sakač Dejanand Vapa Ljiljana

Apoe gene polymorphism in patients with coronary heart disease

Buzadžić Ivana, Pejin Ivana, Ilić V. Tihomir, Janković-Oreščanin Biljana and Šuput-Tomaš Ljiljana

Association of Parkinson’s disease and polymorphism in environment responsive genes

Bošković Marija, Popović Branka and Milašin Jelena

Analysis of the -1562 C>T polymorphism in MMP-9 promoter in patients with head and neck carcinoma

Dobričić Valerija, Novaković Ivana and Kostić Vladimir

Determination of number of CAG repeats within HTT gene by capillary electrophoresis

Denčić-Fekete Marija, Đorđević Vesna, Tiziana Storlazzi Clelia, Janković Gradimir, Bogdanović Andrija, Jovanović Jelica, Rocchi Mariano, Todorić-Živanović Biljana, Strnad Milica and Gotić Mirjana

Translocation (5;6;12) associated with resistance to imatinib mesylate in chronic myeloid leukemia

Mišković Marijana, Lalić Tanja, Radivojević Danijela, Guć-Šćekić Marija, Zamurović Dragan and Vlahović Gordana

Prenatal and postnatal molecular study of SMA using restriction enzyme assay

Durković Jasmina, Cemerlic Dejan, Anđelić Luka and Drakulić Danijela

Survey of a case of gender reversion

Jovičić Dubravka, Rakić Boban, Vukov Tanja, Milačić Snežana, Pajić Jelena, Stevanović Milena and Drakulić Danijela

Comparative cytogenetic analysis of chromosomal aberrations and premature centromere division in lymphocytes of peripheral blood in persons professionally exposed to radionuclides

Daggag Hinda, White Stefan and Sinclair Andrew

Copy number changes in patients with disorders of sex development and functional analyses of candidate genes

Joksić Ivana, Guć-Šćekić Marija, Vujić Dragana, Petrović Sandra, and Leskovac Andreja

Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of fanconi anemia patients

Radivojevic Miloš, Keckarović-Marković Milica, Dacković Jelena, Apostolski Slobodan, Brajušković Goran and Romac Stanka

GLI3 gene molecular analysis of patients with Pallister-Hall syndrome

Lukić Jelena, Anđelković Marina and Bugarić Predrag

Rapid prenatal diagnosis of aneuploidy using quantitative fluorescence-PCR (QF-PCR) in Serbia

Popović Branka, Jekić Biljana, Novaković Ivanaand Milašin Jelena

The role of p53 gene and human papilloma virus in pathogenesis of oral squamous cell carcinomas (OSCCS)

Manasijević Maja, Nešić Branka, Jelovac Drago, Ilić Branislav, Popović Branka and Milašin Jelena

Analysis of c-myc and c-erbB-2 oncogenes in histopathologically tumour-free surgical margins in patients with oral squamous cell carcinoma and basal cell carcinoma

Fatović-Milićević Božica, Mitić-Ćulafić Dragana, Vuković-Gačić Branka, Berisavac Milica, Lakić Nada and Knežević-Vukčević Jelena

Detection of pre-manifested changes of cervix uteri with comet assay

Branković Ljiljana, Milićević Radomir, Živadinović Radomir and Milašin Jelena

Infections with human papilloma virus (HPV) and cytologic changes in the cervical epithelium

Topic IV

Invited lecture

Herrera J. Rene, Somarelli A. Jason, Mesa Annia, Rodriguez E. Carol

Do U1 small nuclear RNA variants mediate differential pre-mRNA splicing?

Introductory lectures

Kobiljski Borislav, Kondić-Špika Ankica, Trkulja Dragana and Brbaklić Ljiljana

Application of molecular markers in wheat breeding

Oral presentations

Golić Nataša, Terzić-Vidojević Amarela, Begović Jelena, Jovčić Branko, Veljović Katarina, Strahinić Ivana, Lozo Jelena, Nikolić Milica, Tolinački Maja and Topisirović Ljubiša

Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum isolated from different ecological niches

Lozo Jelena, Kojić Milan, Jovčić Branko and Topisirović Ljubiša

Molecular characterization of bacteriocins produced by natural isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8

Vojnović Sandra, Ilić-Tomić Tatjana and Vasiljević Branka

Secondary structure within 5’ untranslated region of the kgmB mRNA plantation establishment

Jovčić Branko, Fira Đorđe, Topisirović Ljubiša and Kojić Milan

Biodegradation of alkyl sulfates

Timotijević Gordana, Milisavljević Mira, Samardžić Jelena,Konstantinović Miroslav and Maksimović Vesna

Senescence-associated aspartic proteinase gene from buckwheat

Kalendar Ruslan

Universal amplification method of retrotransposon terminal ltr parts: new tool for polymorphism detection in plant and animal genomes and efficient retrotransposon cloning

Daggag Hinda, Svingen Terje, Western Patrick, van den Bergen Jocelyn, McClive Peter, Harley.Vincent, Koopman Peter and Sinclair Andrew

The Rhox homeobox gene family shows sexually dimorphic and dynamic expression during mouse embryonic gonad development

Mladenović Djordjević Aleksandra, Perović Milka, Smiljanić Kosara, Tešić Vesna, Tanić Nikola, Ruždijić Sabera and Kanazir Selma

Cholesterol homeostasis in the brain- regulation of gene expression in aging and caloric restriction

Tanic Miljana, Zajac Magdalena, Benitez Javier and Martinez-Delgado Beatriz

The role of microRNAs in regulation of BRCA1 gene

Posters

Bugarski-Stanojević Vanja, Stamenković Gorana, Adnađević Tanja, Blagojević Jelena, and Vujošević Mladen

The estimation of phylogenetic relationships among three species of genus Apodemus (Rodentia, Mammalia)

Tasić Srđan and Tasić Irena

Molecular characterisation of Seratia fonticola from oligomineral water

Tasić Srđan and Tasić Irena

Molecular characterisation of Pseudomonas sp. from oligomineral water

Djuric Simonida, Jarak Mirjana and Josic Dragana

RAPD fingerprinting of indigenous Azotobacter isolates from different types of soil

Jošić Dragana, Delić Dušica, Rasulić Nataša, Stajković Olivera, Kuzmanović Đorđe and Miličić Bogić

Phosphate solubilization and (GTG)5 analysis of pseudomonads from rhizosphere

Ivanović Žarko, Gavrilović Veljko, Živković Svetlanaand Stanković Slaviša

REP PCR as a method for determining diversitiy among Pseudomonas syringae strains from fruit trees

Lepšanović Zoricaand Libisch Balázs

Characterization of the metallo-b-lactamase gene from a Pseudomonas aeruginosa clinical isolate

Begović Jelena, Veljović Katarina, Strahinić Ivana and Topisirović Ljubiša

Antibiotic susceptibility of Staphylococcus epidermidis isolated from human milk storage

Jošić Dragana, Kuzmanović Slobodan, Stojanović Saša, Aleksić Goran and Starović Mira

XIIA group phytoplasma detection by RFLP analysis of 16S rDNA sequences

Tolinački Maja, Fira Đorđe, Kojić Milan and Topisirović Ljubiša

Antimicrobial activity of natural isolates of strain Lactobacillus paracasei subsp. paracasei

Veljović Katarina, Terzić-Vidojević Amarela and Topisirović Ljubiša

Antimicrobial and proteolytic activity of chosen Enterococci

Kočiš Tubić Nataša, Obreht Dragana, Pal Boža and Vapa Ljiljana

Microsatellite allelic variability in Ambrosia artemisiifolia L. populations in Vojvodina

Bratić Ana, Majić Dragana, Samardžić Jelena, and Maksimović Vesna

Functional promoter analysis of plant genes in bright yellow 2 cells and pollen grains

Aleksić Jelena, Geburek Thomas, Fussi Barbara and Heinze Berthold

Two mitochondrial group II introns in Picea omorika: New insights into evolutionary relations among spruces

Jevremović Slađana, Stanišić Mariana, Ćalić-Dragosavac Dušica, Raspor Martin, Ninković Slavica, Subotić Angelina, Trifunović Milana and Petrić Marija

Clonal fidelity of Iris sibirica plantlets regenerated by culture in vitro

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Investigations of genetic diversity of Paenibacillus larvae from Serbia using REP-PCR fingerprint technique

Kovačević Nives and Kaeppler Shawn

Chromatin mutants affecting paramutation of r1

Nešić Milica, Kuzmanović Dragana, Jovanović-Ćupić Snežana, Libek Vesna, Pašić Srđan and Stamenković Gorana

Standardisation of methods for detection of virus genome in blood and blood products: our experience with parvovirus B19

Savić Dejana, Kuljić Kapulica Nada and Jovanović Dragutin

The evaluation of PCR method for early detection and monitoring of citomegalovirus infection in transplanted patients

Meier Carste and Berenji Janoš

GM Arabidopsis thaliana for biodetection af landmines

Topic V

Invited lecture

Gömöry Dušan and Paule Ladislav

Traces of evolutionary processes in the gene pool of western-eurasian beeches

Heinze Berthold

Forest molecular genetics: promises, breakthroughs, and the practice

Introductory lectures

Janoš Berenji

New trends in plant breeding

Isajev Vasilije, Orlović Saša, Konstantinov Kosana, Drinić Mladenović Snežana, Lučić Aleksandarand Ivetić Vladan

The use of genetic markers in the enhancement of forest trees seed production in Serbia

Miščević Branislav, Bošković Jelena, Ivanc Aleksandar, Vukosav Marija and Kenjveš Tibor

Biotechnology and genetic improvement of domestic animals

Saftić-Panković Dejana, Radovanović Nataša, Jocić Siniša

MAS of sunflower for the resistance to downy mildew

Stevanović Jevrosima, Stanimirović Zoran, Ninoslav Đelić, Radaković Milena

Parentage verification and sex determination in cattle using molecular markers

Anđelković Violeta, Marković Ksenija, Ignjatović-Micić Dragana, Nikolić Ana, Drinić Mladenović Snežana and Lazić-Jančić Vesna

Maize breeding towards drought tolerance

Boćanski Jan and Savić Ivanov Milena

State and perspective of plant genetic resources in Serbia

KonstantinovKosana, Drinić Mladenović Snežana and IsajevVasilije

Crop, forestry and animal genetic resources – role of biotechnology for the characterization and conservation

Oral presentations

Dimitrijević Miodrag, Petrović Sofija, Banjac Borislav and Vukosavljev Mirjana

Phenotypic reaction of wheat grown on different soil types

Šurlan-Momirović Gordana, Zorić Miroslav, Kovačević Vlado, Prodanović Slaven, Vučković Savo, Maklenović Violeta, Jolanka Marton and Birkas Marta

Impact of global and regional climate changes on maize grain yield

Zečević Bogoljub, Đorđević Radiša, Damnjanović Jelena, Đorđević Mladen, Todorović Goran and Jevđović Radosav

Analysis of influence of parental germplasm on fruits characters of F1, F2 and F3 generations of pepper hybrids (Capsicum annuum L.)

Ellis Noel, Hofer Julie, Mikić Aleksandar, Mihailović Vojislav, Vasiljević Sanja, Milić Dragan, Đorđević Vuk and Perić Vesna

Leaf types in legumes and their agronomic importance

Smýkall Petrl, Kenicer Greg and Mikić Aleksandar

'Beautiful Vavilovia' (Vavilovia formosa) and molecular taxonomy of tribe Fabeae

Radičević Sanja, Cerović Radosav, Glišić Ivana and Karaklajić-Stajić Žaklina

Promising sour cherry hybrids (Prunus cerasus L.) developed at fruit research institute Čačak

Rakonjac Ljubinko, Isajev Vasilije and Lučić Aleksandar

Scots pine (Pinus silvestris L.) ecotypes of the Illyrian region in the afforestation of sites in south-eastern Serbia

Ivetić Vladan, Isajev Vasilije and Drinić Mladenović Snežana

Using of geographical component of genetic data

Galović Vladislava, Orlović Saša, Kovačević Branislav and Pilipović Andrej

Interspecific DNA variation in poplars using AFLP and SSR markers

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Species identification of Nosema microsporidian pathogen in samples of Apis mellifera from Serbia using PCR-RFLP method

Perović Dragan, Günter J., Steffenson B., Kopahnke D., Pržulj Novo and Ordon F.

Genetic mapping of leaf rust (Puccinia hordei Otth) resistance in barley accession MBR1012 originating from R. of Serbia and R. of Montenegro

Dodig Dejan, Kandić Vesna, Zorić Miroslav, Šurlan-Momirović Gordana and Knežević Desimir

The drought responce of wheat landraces from the Western Balkan

Nikolić Ana, Ignjatović-Micić Dragana, Lazić-Jančić Vesna and Pekić Sofija

QTL analysis of traits contributing to drought tolerance in maize

Dimitrieski Miroslav

Transmission of resistance to tobacco mosaic virus (TMV) in some oriental tobacco varieties

Denić Miloje, Ignjatović-Micić Dragana, Stanković Goran, Marković Ksenija, Žilić Slađana, Lazić-Jančić Vesna, Chauque Pedro, Fato Pedro, Mariote David, and Haag Wayne

Role of genetic resources of different geographic origin in simultaneous maize breeding for high protein quality and stress tolerance

Sokolović Dejan, Babić Snežana, Tomić Zorica, Radović Jasmina, Lugić Zoran and Cvetković Mirjana

Broadening of perennial ryegrass (Lolium perenne L.) and cocksfoot (Dactylis glomerata L.) autochthonous genotypes collection

Mratinić Evica, Fotirić Milica

Genetic resources of apple (Malus sp.) in south Serbia region

Longauer Roman, Lavadinović Vera, Gömöry Dušan Paule Ladislav, Borlea Gheorghe, Ballian Dalibor, Brus Robert, Zhelev Peta7 and Andonoski Aleksandar

Genetic variation of european silver fir (Abies alba) in southeastern Europe

Posters

Knežević Desimir Kovačević Vlado, Đukić Nevena, Jelić Miodrag and Luković Kristina

Genotypic variability of grain number per spike in wheat (Triticum aestivum L.)

Đukić Nevena, Knežević Desimir and Stamenković-Radak Marina

Variability of Gli-A2 alleles of 21 Triticum durum culltivars

Olivera Nikolić, Tomislav Živanović, Milovanović Milivoje

Possibilities of using physiological parameters in wheat breeding on grain yield

Petrović Sofija, Dimitrijević Miodrag, Banjac Borislav, Vukosavljev Mirjana

Spike stability parameters in wheat grown on solonetz soil

Jankuloski Ljupcho, Jankulovska Mirjana and Simeonovska Emilija

Induction of doubled haploids in Macedonian wheat genotypes (Triticum aestivum L.)

Prodanović Slaven, Girek Zdenka and Matzk Fritz

Effect of auxin concentration on the growth of wheat haploid embryos

Vukosavljev Mirjana, Kondić-Špika Ankica, Petrović Sofija, Dimitrijević Miodrag, Mladenov Velimir and Banjac Borislav

The effect of androgenic response components on regeneration wheat plants in vitro

Brdar-Jokanović Milka, Kobiljski Borislav, Kraljević-Balalić Marija and Maksimović Ivana

Wheat (Triticum aestivum L.) boron tolerance in field conditions

Kondić-Špika Ankica, Kobiljski Borislav, Marjanović Milica, and Hristov Nikola

In vitro evaluation of boron tolerance in wheat genotypes

Đurić Veselinka, Hristov Nikola, Kondić-Špika Ankica, Aćin Vladimir, Racić Marija

Effect of N mineral fertilization on the alveographic parameters of winter wheat

Jelić Miodrag., Đalović, I.., Knežević Desimir., Paunović Aleksandar

Variation of N, P and K contents in grain and straw of winter wheat genotypes in dependence of system of fertilization

Hristov Nikola, Mladenov Novica, Đurić Veselinka, Kondić-Špika Ankica, Marjanović-Jeromela Ana and Lečić Nada

Effect of fertilization on oil content in wheat grain

Gorjanović Biljana and Kraljević-Balalić Marija

The grain yield of bread wheat genotypes on three levels of nitrogen nutrition

Knežević Desimir, Zečević Veselinka, Đukić Nevena and Konstantinov Kosana

Genetic determination of technological quality of wheat (Triticum aestivum L.)

Zečević Veselinka, Knežević Desimir, Bošković Jelena, Mićanović Danicaand Luković Kristina

Variability of technological quality of winter wheat

Madić Milomirka, Paunović Aleksandar, Knežević Desimir and Zečević Veselinka

Inheritance of stem height and second internode length barley hybrids

Milovanović Milivoje and Perišić Vladimir

Results and perspectives of winter triticale breeding Kragujevac

Babić Vojka, Babić Milosav, Filipović Milomir and Delić Nenad

A visual estimation of the phenotype as an indicator of relatedness of maize inbred lines

Deletić Nebojša, Stojković Slaviša and Gudžić Slaviša

The change of genetic and phenotypic variability of yield components after recurrent selection of maize

Sečanski Mile, Živanović Tomislav, Šurlan-Momirović Gordana, Prodanović Slaven and Jovanović Snežana

Combining abilities for yield maize of lines from different election ciklus

Pajić Zorica, Erić Uroš, Srdić Jelena, Drinić Mladenović Snežana and Filipović Milomir

The mode of inheritance and heritability of the yield and the popping volume in popping maize (Zеа mays L. everta) hybrids

Ivanović Mile, Stanisavljević Dušan, Stojaković Milisav, Treskić Sanja and Mitrović Bojan

An evaluation of the two groups of half-sib families for grain yield in maize (Zea mays L.)

Srećkov Zorana, Nastasić Aleksandra, Ivanović Mile and Boćanski Jan

Correlation and path analysis of grain yield and morphological traits in test-cross populations of maize

Stojković Slaviša, Deletić Nebojša, Biberdžić Milan and Aksić Miroljub

The effect of yield components on grain yield in different progeny types of an F3 maize population

Pavlov Jovan, Stanković Goran and Delić Nenad

The utilisation of related maize inbreds in the hybrid seed production

Filipović Milomir, Čamdžija Zoran and Lopandić Dragiša

The performance of maize hybrids with exotic germplasm under conditions of our country

Babić Milosav, Babić Vojka, Prodanović Slaven and Anđelković Violeta

Yields stability of commercial maize hybrids

Cvarkovic Radomir, Branković Gordana, Calic Irena, Delić Nenad, Živanović Tomislav and Šurlan-Momirović Gordana

Stability of yield and yield components in maize hybrids

Đurović Dragan, Madić Milomirka and Stevović Vladeta

Stability parameters of grain yield in maize hybrids (Zea mays L.)

Vančetović Jelena, Jankuloski Ljupcho, Božinović Sofija and Dodig Dejan

The effects of cytoplasmic male sterility and xenia on the chemical composition of maize grain

Kravić Natalija, Anđelković Violeta, Hadži-Tašković Šukalović Vesna and Vuletić Mirjana

Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm

Ristić Danijela, Ignjatović-Micić Dragana, Drinić Mladenović Snežana, Anđelković Violeta and Nikolić Ana

Comparison of RAPD and SSR markers for genetic diversity studies in maize local populations

Ignjatović-Micić Dragana, Marković Ksenija, Drinić Mladenović Snežana and Lazić-Jančić Vesna

SSR polymorphism assay for modifier loci in normal and opaque2 maize inbred lines

Kovačević Vlado and Šimić Domagoj

Heredity impacts on phosphorus and potassium concentrations in maize

Drinić Mladenović Snežana, Ristić Danijela, Sredojević Slobodanka, Dragičević Vesna, Terzić Dušanka, Nenad Delić and Ignjatović-Micić Dragana

Genetic variation of phytate in maize population

Radosavljević Milica, Mojović Ljiljana, Semenčenko Valentina and Milašinović Marija

The utility value of the grain of maize hybrids of a different genetic background

Atlagić Jovanka, Marjanović-Jeromela Ana, Marinković Radovan and Terzić Sreten

Pollen traits of the oil species from the Novi sad collection

Drumeva Miglena, Yankov Peter and Nenova Nina

New sunflower restorer lines developed by y-induced parthenogenesis from Helianthus annuus hybrids - disease resistance, combining ability. II. Combining ability.

Hladni Nada, Jocić Siniša, Miklič Vladimir, Saftić-Panković Dejana and Kraljević-Balalić Marija

Interdependence between yield and yield components of the confectionary sunflower

Marinković Radovan, Marjanović-Jeromela Ana, Radić Velimir

Genetics of some quantitative traits in sunflower (H. annuus L.)

Miladinović Dragana, Miladinović Jegor, Siniša Jocić and Miklič Vladimir

The effect of oxalic acid on asymmetry of somatic hybrids between cultivated sunflower and Helianthus maximiliani

Cvejić Sandra and Jocić Siniša

Development of hybrids of ornamental sunflower

Srebrić Mirjana, Kovačević Dragan and Perić Vesna

Relathionsip between conventional and kunitz free soybean lines in early generation selection

Perić Vesna, Nikolić Ana and Drinić Mladenović Snežana

Genetic diversity of soybean genotypes based on RAPD markers

Miladinović Jegor, Đorđević Vuk, Balešević-Tubić Svetlana and Miladinović Dragana

Genetic diversity of soybean genotypes originating from distant geographical regions

Perić Vesna, Srebrić Mirjana, Jankuloski Ljupcho, Mirjana Jankulovska, Žilić Slađana and Drinić Mladenović Snežana

The effects of nitrogen on protein and trypsin inhibitor content of soybean

Srebrić Mirjana, Perić Vesna, Dragičević Vesna, Sredojević Slobodanka, Terzić Dušanka and Drinić Mladenović Snežana

Genetic variability of phosphorius content in soybean seed

Marjanović-Jeromela Ana, Marinković Radovan, Miladinović Dragana, Stojaković Željka and Terzić Sreten

Inheritance of tocopherol contents in seeds of rapeseed (Brassica napus L.)

Danojević Dario, Kovačev Lazar, Ćurčić Živko, Nagl Nevena, Rajić Milorad and Stojaković Željka

Variability of root and seed traits in sugar beet

Terzić Sreten and Atlagić Jovanka

Nitrogen and sugar content variability in tubers of Jerusalem artichoke ( Helianthus tuberosus )

Korubin-Aleksoska Ana, Aleksoski Jane

Combining quantitative characters in breeding the lines of Nicotiana tabacum L. with diallel crossings

Miceska Gordana

Determination of the level of androgenesis in tobacco

Zdravković Jasmina, Pavlović Nenad, Girek Zdenka, Zdravković Milan and Cvikić Dejan

Characteristics important for organic breeding of vegetable crops

Kondić Svetlana, Takač Adam, Gvozdanović-Varga Jelica, Vasić Mirjana and Červenski Janko

Tomato ( Lycopersicum esculentum Mill. ) genotypes variability for the fruit traits

Živanović Tomislav, Prodanović Slaven, Šurlan-Momirović Gordana and Krstanović Saša

Genetic variability components of some quantitative traits of tomato

Pavlović Nenad, Cvikić Dejan, Zdravković Jasmina, Mijatović Mirjana and Brdar-Jokanović Milka

Dry matter content inheritance pattern in fresh bulb onion (Allium cepa L.)

Poštić Dobrivoj, Sabovljević Radovan and Bogdanović Zorica

Indicators viability of potatoe seed-tuber

Đorđević Radiša, Zečević Bogoljub, Damnjanović Jelena, Todorović Goran and Jevđović Radosav

Influence of af gene on the grain number of pea (Pisum sativum L.) per pod

Damnjanović Jelena, Zečević Bogoljub, Đorđević Radiša, Đorđević Mladen and Todorović Goran

Genetic analysis of inheriting the eggplant ( Solanum melongena L.) fruit widthh

Smýkall Petrl, Jarkovský J., Corander J. and Hýbl M.

Genetic diversity and population structure among pea (Pisum sativum L.) varieties revealed by combined marker analysis based upon retrotransposons, microsatellites and morphological characters

Mikić Aleksandar, Mihailović Vojislav, Ćupina Branko, Katić Slobodan, Vasiljević Sanja, Milić Dragan and Krstić Đorđe

Achievements in white lupin (Lupinus albus L.) breeding in Serbia

Radović Jasmina, Štrbanović Ratibor, Lugić Zoran, Sokolović Dejan and Vasić Tanja

Variability of morphological traits and dry matter yield of birdsfoot trefoil (Lotus corniculatus L.) wild populations

Mihailović Vojislav, Mikić Aleksandar, Dimitrijević Miodrag, Petrović Sofija and Katić Slobodan

Interspecies hybridisation in some annual legumes

Babić Snežana, Sokolović Dejan, Anđelković Snežana, Štrbanović Ratibor and Živković B.

Variability of the most important traits of timothy (Phleum pratense L.) autochthonous populations

Katić Slobodan, Milić Dragan, Mihailović Vojislav, Vasiljević Sanja and Mikić Aleksandar

Effects of heterosis for yield and yield components obtained by crossing divergent alfalfa populations

Lugić Zoran, Radović Jasmina, Sokolović Dejan, Anđelković Snežana, Babić Snežana

Genotype potential of different origins of white clover (Trifolium repens L.) for the basic agronomic features

Zec Gordan and Čolić Slavica

Influence of vineyard peach selection`s F1 generation to vigour and yield in peach autumn glo and nectarine stark red gold

Rakonjac Vera, Nikolić Dragan and Fotirić Milica

Genetic gain from selection of vineyard peach native population

Marić Slađana, Lukić Milan and Bošković Radovan

Polymorphism, inheritance and mapping of ETR1 gene in apple (Malus x domestica Borkh.)

Živković Svetlana, Stojanović Saša, Ivanović Žarko, Gavrilović Veljko and Balaž Jelica

Morphological and molecular analysis of Colletotrichum species from pear

Miletić Rade

Correlation between the onset of vegetation growth and major properties of walnut fruits

Miletić Rade

Correlation between the end of growing season and major fruit properties in a walnut population

Čolić Slavica, Milatović Dragan, Nikolić Dragan and Zec Gordan

Dehydrogenase isoenzyme polymorphism in selected almond genotypes (Prunus amygdalus Batsch)

Nikolić Mihailo, Radović Aleksandar, Fotirić Milica, Milivojević Jasminka and Nikolić Dragan

Pomological properties of promising raspberry seedlings with yellow fruits

Nikolić Dragan

Inheritance of some grapevine properties in crossing combination of seedling 113 x muscat hamburg

Šijačić-Nikolić Mirjana, Milovanović Jelena, Savić-Pavićević Dušanka, Brajušković Goran and Diklić Miloš

Sessile oak different regions genetic distance in Serbia

Šijačić-Nikolić Mirjana, Milovanović Jelena, Pilipović Andrej and Orlović Saša
Plants survive variability of different beech provenances within the serie of European provenance tests in Serbia

Lavadinović Vera, Isajev Vasilije and Radošević Gordana

Interdependence of width of needles and sites of douglas-fir provenances in test plantations in Serbia

Mrsic Marina and Pavlović P. Boro

Correlations of seed properties with its position inside seed cone of Abies alba Mill.

Lučić Aleksandar, Drinić Mladenović Snežana, Isajev Vasilije and Rakonjac Ljubinko

Polymorphism of seed proteins in populations of Scots pine (Pinus silvestris L.) in Serbia

Daničić Vanja, Isajev Vasilije, Mataruga Mila and Cvjetković Branislav

Inter-clonal variability of physiological properties of Scots pine (Pinus silvestris L) pollen

Ivetić Vladan

Use of GIS – from making conservation strategies to seed source selection

Andrić Andrijana, Obreht Dragana, Rat Milica, Pal Boža and Vujić Ante

Analysis of genetic variability in Ornithogalum L. using RAPD markers

Sedlovskiy А.I., Tupina L.N., Kоkhmetova А.М. and Baimagambetova К.К.

The screening of wheat germplasm to resistance to leaf rust in Kazakhstan

Prijić Željana and Jerković Zoran

Common to Puccinia triticina durable resistance genes combinations in Serbia

Staletić Mirjana, Milovanović Milivoje, Perišić Vladimir and Đekić Vera

Efficiency of Pc genes of resistance to crown rust of oat

Bekavac Goran, Purar Božana, Jocković Đorđe, Treskić Sanja, Stanisavljević Dušan and Mitrović Bojan

Genetic analysis for corn reddening tolerance in broad-base population NS 1-257 CRS

Radovanović Nataša, Jocić Siniša, Miklič Vladimir and Saftić-Panković Dejana

Changes in the Pl6 genomic region for the resistance to downy mildew in recombinant sunflower inbred lines

Mezei Snežana, Kovačev Lazar, Atlagić Jovanka and Nagl Nevena

Meiosis in diploid sugar beet populations rezistant to rhizomania

Živić Jelica, Knežević Desimir, Petrović Saša and Stančić Ivica

Variability of contents of nonsugar matter in rot of sugarbeet Beta vulgaris var. saccharifera at multigerme fertilizers in condition of rhizomania infection

Oro Violeta and Ivanović Žarko

Evaluation of DNA extraction methods in Globodera pallida PCR identification

Oro Violeta

Potato root pathocytologic changes and Globodera pallida (Nematoda: Heteroderidae) parasitism

Kovačević Branislav, Drekić Milan, Orlović Saša and Poljaković-Pajnik Leopold

Variability and relationship among characters of tolerance in black poplar clones to Phyllodecta vitellinae L.

Marković Ksenija, Kravić Natalija, Anđelković Violeta, Vuletić Mirjana

Response of maize genotypes with different proportion of exotic germplasm to osmotic stress

Simić Milena, Filipović Milomir, Dolijanović Željko, Grčić Nikola

The importance of genotypes for the increase of the competitive ability of maize against weeds

Dražić Slobodan, Dajić Zora and Živanović Tomislav

Diversity and utilization of medicinal plants genetic resources

Ilić Zoran, Milenković Lidija, Vasić Mirjana and Đurovka Mihal

Old cultivars and populations from traditional pepper-growing regions of Serbia

Taški-Ajduković Ksenija, Knoblauch Rainer, Nagl Nevena, Jevtić Aranka and Vujaković Milka

Genetic purity in seed testing using biomolecular markers

Sredojević Slobodanka, Dragičević Vesna, Srebrić Mirjana, Milivojević Marija and Đukanović Danica

The posibility of thermodynamic’s application in seed characterisation 1. Gibbs’ free energy.

Dragičević Vesna, Kobiljski Borislav, Radojčić Aleksandar, Dodig Dejan, Kandić Vesna, and Sredojević Slobodanka

The posibility of thermodynamic’s application in seed characterisation 2. Monocotyledon plants.

Nišavić Anika, Perić Vesna, Srebrić Mirjana, Đukanović Lana, Dragičević Vesnaand Sredojević Slobodanka

The posibility of thermodynamic’s application in seed characterisation 3. Dicotiledon plants.

Bošković Jelena, Isajev Vasilije, Miščević Branislav, Ivanc Aleksandar, Zečević Veselinka, Dozet Gordana

Interactions of gentically modified crops and environment

Milovanović Milivoje, Perišić Vladimir and Staletić Mirjana

Žarko – Perspective winter triticale cultivar

Perišić Vladimir, Milovanović Milivoje, Staletić Mirjana, Nikolić Olivera

Delija - New spring Triticale cultivar

ROUND TABLE I

Cvetković Dragana

Darwin’s legacy and modern genetics

Brockett Milosevic Mirjana

Strategies for teaching genetics and evolution more effectively

ROUND TABLE II

Marinkovic Dragoslav

Bioethics in science and medicine in R. Serbia

Magić Zvonko

Legislation of genetic testing in medicine

Konstantinov Kosana and Drinić Mladenović Snežana

Ethical aspects of genes and organisms protected by patent

Drinić Mladenović Snežana and Konstantinov Kosana

40 years of the journal Genetika

Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.6

FROM GENES TO GENOMES

Glišin Vladimir

Institute of Molecular Genetics and Genetic Engineering, Belgrade

The advent of our capabilities to sequence whole genomes of hundreds of organisms from bacteria to human, as well as to monitoring in a real time and in its totality the biochemical functionality of these genomes in different physiological, developmental, pathological, evolutionary settings, has significantly changed our tenet on molecular biology from the time of Watson’s Molecular Biology of the Gene (1965) to Molecular Biology of the Cell (2008). The old conventional wisdom said that to get a gene expressed, the DNA of that gene was copied into RNA, and that would be sent to the ribosome to be made into protein with the help of three RNAs. Now, everywhere you look there is some new kind of RNA with some known and unknown kind of function. Furthermore, there is a change how we view the genomes, particularly concerning how the genomes are assembled and how that information is translated to become proteins. Also, until recently, one supposed that structural variations within the genome of a species even within an individual, were not so common, but now there are more structural variations than we have ever expected. In my presentation I shall discuss and document the progress in the understanding of all biological processes based on genomics rather than on genetics.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.8

EVOLUTIONARY CONSERVATION GENETICS IN PRACTICE - IDENTIFICATION OF UNIQUE ENTITIES IN THE FAMILY Syrphidae

Milankov Vesna and Ludoški Jasmina

Department for biology and ecology, Faculty of Science,

University of Novi Sad, Novi Sad, Serbia

Delimitation of biological diversity is a crucial step in understanding phenomena in evolutionary biology, conservation biology, ecology, and biogeography. For identification of areas of genetic endemism of taxonomically diverse and challenging taxa, such as the family Syrphidae, it is important to delineate genetic units that are geographically discrete, often characterized by non-overlapping haplotype distributions and/or occurrence of unique allozyme markers. Identification of genetic uniqueness and genetic endemism emphasizes the importance of integration of multiple characters (mitochondrial sequence data, allozyme loci and quantitative traits) and estimation of genetic and phenotypic diversity in recognition of conservation units. Based on the observed private haplotypes, alleles and genotypes, unique heterozygotes, significant differences of allelic frequencies and recognized cryptic species across the full hierarchy of taxonomic and genetic diversity, molecular markers and wing geometric morphometrics are a suitable representation of the evolutionary potential of the hoverflies on the Balkan Peninsula.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.10

ARE B CHROMOSOMES GENOMIC PARASITES? – GEOMETRIC MORPHOMETRIC ANALYSIS OF THE CANALIZATION AND DEVELOPMENTAL STABILITY IN YELLOW-NECKED FIELD MICE

Apodemus flavicollis (Rodentia, Mammalia)

Jojić Šipetić Vida, Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

The objective of this study was to test currently favored (parasitic) model of B chromosomes (Bs) maintenance. Bs, as true parasites, would increase level of stress in the host, i.e. B carriers would have lower levels of both canalization and developmental stability compared to non-carriers. To test this hypothesis 60 mandibles and 146 crania of adult yellow-necked field mice (Apodemus flavicollis) collected during one year on Mt. Jastrebac were analyzed. Size and shape variance among individuals and fluctuating asymmetry (FA) were estimated using geometric morphometric methods. B carriers had a higher variance for cranium shape, although without statistically significant difference. Non-carriers showed significantly higher level of FA for cranium shape compared to B carriers. The fact that B chromosomes do not decrease the levels of canalization and developmental stability of analyzed skull features in studied population of A. flavicollis should not be considered as a conclusive argument for accepting some of the alternative models of Bs maintenance, but only as the evidence for rejecting herein tested parasitic model.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.11

MICROSATELLITE ANALYSIS OF GENETIC DIVERSITY IN WILD BOAR

Veličković Nevena¹, Đan Mihajla¹, Obreht Dragana¹, Zorić Miroslav²and Vapa Ljiljana¹

¹Department for biology and ecology, Faculty of Science,University of Novi Sad

²Facultyof agriculture, Belgrade, Serbia

The aim of this study was the assessment of genetic diversity in the captive wild boar population from Danube region. A total of 51 muscle tissue samples were collected. A set of five microsatellite markers, developed for domestic pig, was used. Amplification was successful at 4 of them, and all four loci were included in statistical analysis since linkage disequilibrium test showed that loci were not associated. A total number of 59 alleles were found, with an average of 14.7 alleles/locus. The highest number of alleles per locus was found at S0068 microsatellite (24), while the lowest number (9) was present at locus SW251. Group-specific numbers of allele was higher in young (55) then in adults (26). Among all alleles, 22 (37.29%) were found in both analyzed groups. All others were private. Average observed heterozygosity (Ho) values for population was 0.579 (0.348-0.833). Similar values were found for both groups. All F_IS values were significantly higher then zero, which reflects a substantial deficit of heterozygotes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.12

LARGE-SCALE DIFFERENTIATION OF WILD BOAR (Sus scrofa) POPULATIONS: IS THERE INTRASPECIFIC STRUCTURE?

Paule Ladislav¹, Bakan Jana¹, Kuehn Ralph², Nikolov Ivan², Romšáková Ivana¹

and Krajmerová Diana¹

¹Faculty of Forestry, Technical University, Zvolen, Slovakia

²Technische Universität München, Freising, Germany

Wild boar (Sus scrofa) distribution covers the entire continental Europe; it is missing in British Isles, Scandinavia and northern part of the European Russia. The intraspecific taxonomy recognizes numerous subspecies, while in central and south-eastern Europe five subspecies should occur: S.s. scrofa, S.s. atilla, S.s. lybicus, S.s. meridionalis and S. s. algira. Fourteen microsatellites of nuclear DNA composed in four multiplexes: (1) S008, SW986, SW1129; (2) SW1701, SW828, SW1517; (3) SW1465, SW1492, SW1514, SW2532; (4) SW461, SW841, SW2021, SW2496 were used to study the genetic structure and differentiation of the central and south-eastern European wild boar populations and to search for links with the occurrence of individual subspecies. Sharp differentiation of wild boar populations was between Slovakia and the Czech Republic and also between the southern and northern Bulgarian populations, which might indicate that in Western Slovakia and Bulgaria are suture zones of genetic lineages.

The paper was financially supported by research grant APVV-18-032105.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.13

GENETIC fragmentation OF Rupicapra rupicapra balcanica POPULATIONS ALONG THE BALKAN PENINSULA

Paule Ladislav¹, Grubešić Marijan², Krajmerová Diana¹, Tomljanović Kristijan², Ballian Dalibor³ and Zhelev Petar⁴

¹ Faculty of Forestry, Technical University, Zvolen, Slovakia;²Šumarski fakultet, Zagreb, Croatia;³ Šumarski fakultet, Sarajevo, Bosnia and Herzegovina;⁴ University of Forestry, Sofia, Bulgaria

Distribution range of R.r. balcanica stretches from Slovenia to Greece and at present it is discontinuous. We have studied the genetic diversity and differentiation of chamois populations along the Balkan Peninsula with the aim to find the degree of admixture of chamois of Alpine origin (R.r. rupicapra) in Western Balkan. Nuclear microsatellites according to Muntwyler et al. (2002) were used for characterization of genetic diversity and differentiation of individual subpopulations and to define the mixed populations. Northern Croatian populations from Risnjak and Kupa proved to belong to Alpine genetic type together with Slovenian populations. Populations from Biokovo in Croatia and Devin in Bulgaria form another genetic group while Velebit populations with several Bosnian individuals contained both genetic types. Consequences for gene conservation and population management are given.

The paper was financially supported by research grant APVV-18-032105.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.14

“SEX RATIO IN Drosophila pseudoobscura: EVOLUTIONARY ANALYSIS

OF SR POLYMORPHISM”

Brockett Milosevic Mirjana and Anderson W. Wyatt*

School of Biology, Georgia Institute of Technology, Atlanta, GA, USA

*Department of Genetics, University of Georgia, Athens, GA, USA

Sex ratio (SR) condition has been described in the Drosophila literature as a case of almost entirely female offspring produced by certain wild females. In this study, we have focused on the SR trait in D. pseudoobscura, a species from Western coast of the United States and Canada. In this species the SR chromosome is manifested with three inversions on the right arm of X chromosome. SR chromosome never reaches fixation in nature, while despite some selective disadvantages, SR inversions remain widespread within the genus. We have tested some models for selective maintenance of the Sex ratio polymorphism, both in laboratory and natural populations. Our results indicate that in D. pseudoobscura there seems to be frequency dependent gamete selection, responsible for maintenance of the SR genotypes.

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Book of Abstratcts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.15

“GEOGRAPHICAL CHANGES IN RELATIVE FREQUENCY OF INVERSIONS IN CHROMOSOME III OF Drosophila pseudoobscura AMONG NATURAL POPULATIONS FROM MEXICO”

Salceda M. Víctor

Departamento de Biología. Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca S/N, La Marquesa, Ocoyoacac, Mexico

Chromosomal polymorphism in natural populations of Drosophila pseudoobscura have been broadly studied in the USA but scarcely in Mexico with only about 60 localities analyzed. Differences among both regions are notorious with respect to their chromosomal constitution. Northern populations, those of USA, have as representative inversions the sequences ST, AR and CH contrasting with those in Southern populations (Mexico) in which prevail the gene arrangements TL, CU and SC. Assuming as a probable mechanism that has allowed these substitutions the flow generated by the presence of a North-South clines, we took as a goal find out if such clines really exist. With that objective in mind we studied 29 populations of this species distributed along four North-South transects. Specimens of D. pseudoobscura caught by attracting them with fermenting bananas, carried to the laboratory where from each female an isofemale line was established. When their offspring appeared a single larva from each isofemale was taken, its salivary glands extracted and stained with a solution of lacto-aceto-orcein, by these means the polytene chromosomes were obtained. On these chromosomes we identified, for each larva, the inversion (s) carried in the third chromosome, in such a way 3439 third chromosomes were analyzed. Among the 29 localities we identified 17 different inversions but the number of them varied from population to population from three to eleven. Relative frequencies of each inversion at every location were calculated and with them for each transect the presence or absence of clines was determined. Among each transect the existence of clines was observed only between two or three near by populations, but we were not able to find a clear manifestation of the presence of clines along a complete transect. Our results at this respect are similar to those previously reported for USA populations. A mechanism that explains North-South substitutions of predominant inversions remains as open question.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.16

CONSEQUENCES OF OUTBREEDING ON DEVELOPMENTAL STABILITY IN Drosophila subobscura WINGS

Kurbalija Zorana¹, Stamenković-Radak Marina^1,2 and Anđelković Marko^1,2

¹Institute for biological research "Sinisa Stanković“, Belgrade, University of Belgrade, Belgrade; ² Faculty of Biology, University of Belgrade, Belgrade

Fluctuating asymmetry (FA), the increased variation of bilateral symmetry in a sample of individuals, can point to disturbance in developmental stability at molecular, chromosomal and epigenetic level. The aim was to study different genetic causes of FA, such as interpopulation hybridization as genomic stress and find out weather co-adaptations or heterozygosity are probable mechanisms which maintain developmental homeostasis in populations. We focused on co-adaptive aspect of inversion polymorphism in Drosophila subobscura and it’s relation with genomic stress, such as interpopulation hybridization and developmental stability. We analysed the effect of interpopulation hybridization through the variability of fluctuating asymmetry (FA) of wing size parameters (wing width and wing length) of D.subobscura from three ecollogically distinct populations, presuming that they possess a certain degree of genetic differentiation due to their different evolutionary histories. The FA analysis and statistics was done on samples from P, F1 and F2 generations of inter- and intrapopulation crossings.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.17

PRODUCTION OF ANTIMICROBIAL SUBSTANCES AND GENETIC DIVERSITY OF Bacillus sp. STRAINS ISOLATED FROM DIFFERENT HABITATS IN SERBIA

Berić Tanja¹, Fira Đorđe², Stanković Slaviša¹, Urdaci C. Maria³

and Knežević-Vukčević Jelena¹

¹Faculty of Biology,Department of microbiology, University of Belgrade;²Faculty of Biology,Department for biochemistry and molecular biology, University of Belgrade , Serbia;³Labaratory for microbiology and biochemistry application, ENITA de Bordeaux, Gradignan, France

In this study, genetic diversity and production of antimicrobial substances (AMS) of 192 Bacillus strains isolated from different geographical and ecological niches in Serbia was studied. Combining RAPD analysis and 16S DNA sequencing we determined thirteen different groups of RAPD profiles within four species. B. pumilus was represented with three groups of different RAPD profiles, B. subtilis with five, B. cereus with four and B. firmus with one. Production of AMS was tested against plant pathogens: Burkholderia glumae, B. cepacia, Erwinia carotovora, Pseudomonas fuscovaginae and Xanthomonas oryzae. Out of 192 strains, 128 showed some degree of antimicrobial activity against at least one indicator strain, and 42 strains exhibited effect on three and more pathogens.No apparent association between RAPD profiles and the geographic origin or habitat of the strains tested was detected. Antimicrobial activity was related to species and RAPD group. Most AMS producers including the highly potent ones belonged to B. subtilis species.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.20

DISTRIBUTION OF B CHROMOSOMES IN AGE CATEGORIES OF YELLOW-NECKED MOUSE APODEMUS FLAVICOLLIS (MAMMALIA, RODENTIA)

Vujošević Mladen, Blagojević Jelena, Jojić Šipetić Vida, Bugarski-Stanojević Vanja, Adnađević Tanja and Stamenković Gorana

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Supernumerary chromosomes, found in many species, are named B chromosomes (Bs) if they satisfy three criteria: dispensability, absence of paring with A chromosomes and non-Mendelian inheritance. Bs are frequently found in populations of yellow-necked mice, Apodemus flavicollis. Maintenance of Bs in populations is explained by two opposed models. Model signed parasitic claims that Bs are maintained by mechanism of accumulation despite detrimental effects on carrier fitness. On the other hand, heterotic model suggests that, in the absence of accumulation mechanism, small number of Bs could confer an adaptive advantage to carriers, while large number could be deleterious. With the aim of contributing to understanding the mechanisms of Bs maintenance in populations of A. flavicollis, differences in frequencies of Bs in age categories were analysed in 732 animals. Bs described as parasites reduce mean fitness of individuals carrying them. Therefore, it is expected that frequency of animals with Bs will decrease with age increase. Absence of significant statistical differences in frequency of Bs between six age categories point to the heterotic way of Bs maintenance in A. flavicollis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.21

GENOTYPING OF FRUŠKA GORA POPULATION OF YELLOW-NECKED MOUSE APODEMUS FLAVICOLLIS (RODENTIA, MAMMALIA) – AFLP AND B CHROMOSOMES

Adnađević Tanja, Bugarski-Stanojević Vanja, Stamenković Gorana,

Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

B chromosomes (Bs) are extra chromosomes to the standard complement that occur in many organisms. Because of high degree of homology with A chromosome, Bs of Apodemus flavicollis can not be accurately distinguished from chromosomes of normal complement at cytogenetic level, but there are differences at molecular level. AFLP is PCR-based technique that is useful for the rapid screening of genetic diversity. It generates hundreds of highly replicable markers, thus allowing high-resolution genotyping. We investigated usefulness of AFLP markers for genotyping mice in the context of Bs presence or absence. Total of 75 individuals were included in the study and 16 of them are B carriers, all captured on Mt. Fruška Gora. Out of 22, 14 primer combinations gave clear and reproducible pattern. Presence or absence of DNA fragments were transformed to binary data (1, 0). The computing of binary data was performed using MVSP software and genetic distances were estimated by Nei & Li's coefficient and UPGMA clustering. High level of detected intrapopulation polymorphism makes AFLP markers useful tool for genotyping analysis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.22

ANALYSIS OF HYPERVARIABLE DOMAIN 1 OF CONTROL REGION mtDNA IN BROWN HARE POPULATIONS (Lepus europaeus pallas)

Đan Mihajla¹, Obreht Dragana¹, Tanurdžić Miloš², Vapa Milan³ and Vapa Ljiljana¹

¹Faculty of Sciences, Dept of Biology and Ecology, Novi Sad; ²Cold Spring Harbor Lab, New York, USA; ³Faculty of Agriculture, Novi Sad, Serbia

Hypervariable domain 1 of control region of mtDNA is known molecular marker in population genetics studies of Lepus. The aim of this research is to analyse mtDNA CR-1 sequence variation in brown hares from Serbia. In total 157 hares from six regions (Backa, Banat, Srem, Eastern, Central and Western Serbia) were analysed. After sequencing of mtDNA CR-1, subset of 92 sequences (426-432bp) was used for further analyses. Among those 45 haplotypes were found. Unique haplotypes were detected in each population. The highest number of unique haplotypes was present in Central Serbia (9). Haplotype diversity for all populations was Hd=0.944, and nucleotide diversity was π=0.0236. Based on number of nucleotide differencies (Kxy) and genetic divergence (Fst) NJ dendograms were constructed. Both dendograms showed similar branch topology, indicating some separation between two groups of populations, first consists of three populations from Vojvodina and second consists of three populations from Eastern, Central and Western Serbia. The most genetically divergent population was the Western Serbia with two unique sequences detected for the first time in L. europaeus.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.23

OPTIMISATION AND STANDARDIZATION OF PRIMERS FOR STERLET (Acipenser ruthenus) AND BELUGA (Huso huso) MICROSATELLITE LOCI

Cvijanović Gorčin¹, Adnađević Tanja², Bugarski-Stanojević Vanja²

and Lenhardt Mirjana¹

¹ Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Belgrade, Serbia;² Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Overharvest, habitat loss, fragmentation and other anthropogenic factors have resulted in population and distribution decline of both sterlet (Acipenser ruthenus) and beluga (Huso huso) in Danube basen. As all living sturgeon species are endangered and there are numerous conservation programs, there is a need for estimation of genetic diversity and the evolutonary relationships among populations. Microsatellite loci are useful tool for detection of intraspecies and intrapopulatoin polymorphism and numerous microsatellite loci were detected for sturgeon species. Primers for loci from Afu, Aox and Spl groupe were preveously developed for lake sturgeon (Acipenser fulvescens), Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and shovelnose sturgeon (Scaphirhynchus platorynchus) respectfuly and provided a much needed group of genetic markers, detectable with non-invasive sampling (anal fin tissue). We tested and optimised primers for ten microsatellite loci on 10 sterlet and 5 beluga speciments from Serbian part of Danube River. All of tested loci show high level of polymorphism among sample speciments.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.24

GENETIC POLYMORPHISM OF SALICORNIA EUROPAEA GROUP (Chenopodiaceae)

Polić Dubravka, Veselić Sanja, Đan Mihajla and Obreht Dragana

Department of Biology and Ecology, University of Novi Sad,

Faculty of Sciences, Novi Sad, Serbia

In order to estimate the genetic polymorphism of the Salicornia, seven different RAPD (Random Amplified Polymorphic DNA) primers were assayed for four isolated populations from different European regions (Saladar de Aquamarga -Spain, Slano Kopovo -Serbia, Ulcinj salina- Montenegro and Kalloni salina- Lesvos). The screened RAPD primers yielded 58 polymorphic bands: five for primer OPB11, seven for OPB06 and OPB12, eight for K01 and K15, ten for OPA01 and 15 for M02. Values of pairwise comparisons of Nei and Li genetic distance between populations ranged from D=0.469 (Serbia-Spain) to D=0.750 (Serbia-Montenegro). The study indicated that populations from Spain and Serbia are closely related with each other while Montenegro group is different from the both Serbia and Spain groups, which led to conclusion that the Montenegro group belongs to other population which might derived from an ancestry different from that of the Spain and Serbia population. On the other hand, the study pointed out that Lesvos group is quite different from all the other investigated populations.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.25

PRELIMINARY RESULTS OF MOLECULAR GENETIC ANALYSES OF THE GENUS Branchipus schaeffer 1766 (Crustacea, Branchiopoda)

Miličić Dragana, Petrov Brigita, Stojković Biljana and Pavković-Lučić Sofija

Institute of Zoology, Faculty of Biology, University of Belgrade,

Belgrade, Serbia

To gain insights into the taxonomic relationships among different Branchipus populations, molecular genetic analyses were performed. Data matrices used in the analyses included 629 base pairs from the mitochondrial gene COI. We used specimens from Pannonian populations (both Hungarian and Serbian), as well as from localities in Eastern Serbia. Samples of Branchipus intermedius (from Macedonia) were used as outgroup. Both the NJ tree based on p-distances and the MP tree on maximum parsimony were calculated. Results from both methods indicated major divergence between two lineages: the lineage from Pannonian populations, and Eastern Serbia lineage. These preliminary results suggest significant evolutionary branching of the “southern” lineage (from Eastern Serbia), and, consequently, possible higher taxonomic level of this population group. Further analyses are needed for reaching consistent conclusion.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.26

MATING SUCCESS AND WING MORPHOLOGY OF Drosophila subobscura FROM ECOLOGICALLY DIFFERENT ENVIRONMENTS

Savić Tatjana¹, Stamenković-Radak Marina^1,2, Savić Marija², Kenig Bojan¹

and Anđelković Marko^1,2

¹Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Drosophila species exhibit genetic and phenotipic variation in traits affecting mate choice. This variation forms the basis for sexual selection that may lead to isolation between populations in ecologically different environment. Mating success of the flies in different Drosophila species depends on a variety of factors including male courtship vigour, female receptivity, male size and other signals emitted during the courtship.Ecological speciation is commonly considered in terms of pre-mating isolation. In context of this, morphological variation of wing size and shape in ecologically different environments may cause intraspecific variation.Drosophila subobscura provide excellent model system to compare frequency of homo- and heterogametic maiting among populations in divergent environments. Mate preference functions and wing morphology can further be used to develop predictions of relative mating success in population hybridizations.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.27

A LACK OF GENETIC VARIATION IS ASSOCATED WITH ASYMMETRY OF WING TRAITS IN NORTHERN HOVERFLY SPECIES (Diptera, Syrphidae)

Milankov Vesna¹, Ludoški Jasmina¹, Francuski Lubinka¹, Ståhls Gunilla2 and Vujić Ante¹

¹Department for biology and ecology, Faculty of Science,University of Novi Sad, Novi Sad, Serbia;²Finnish Museum of Natural History, University of Helsinki, Finland

The molecular and phenotypic diversity of the Cheilosia aff. longula and C. naruska species (Diptera, Syrphidae) from Lapland, Finland, were examined through DNA sequencing, protein electrophoresis and geometric morphometrics. The morphological identification of the species was verified molecularly using partial sequences of mitochondrial COI and the nuclear ribosomal ITS2 genes comparing with corresponding sequences of previously published of C. aff. longula and closely related C. longula as well as corresponding sequences of a paratype of C. naruska. Analysis of isozyme loci showed an extremely low genetic variability in the studied populations. Variation among genetically diverse individuals was studied both within- and among studied populations, and directional (DA) and fluctuating asymmetry (FA) were estimated using landmarks in the framework of geometric morphometric methods. The documented DA and FA asymmetry in both wing shape and size likely reflected developmental instability of studied individuals. The observed consistent association between high asymmetry of wing traits and low heterozygosity would indicate a reduced potential of populations for adaptive evolution.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.28

CHROMOSOMAL INVERSION POLYMORPHISM IN Drosophila subobscura POPULATION FROM THE GORGE OF THE DERVENTA RIVER

(TARA MOUNTAIN)

Jelić Mihailo¹, Kurbalija Zorana², Kenig Bojan², Stamenković-Radak Marina^1,2

and Anđelković Marko^1,2

¹Faculty of Biology, University of Belgrade, Belgrade; ²Institute for biological research "Sinisa Stanković“, Belgrade, University of Belgrade, Belgrade,Serbia

Due to geomorphological characteristics and climatic conditions, gorges are considered to have been refugia during last glaciation. Therefore, the aim of this research was to determine wheter genetic variability of Drosophila subobscura population from the gorge of the Derventa river (Tara Mountain) shows some local distinctivnes. What makes the Tara National Park especially valuable is the fact that this area is a refugium with complex forest phytocoenoses in which endemics and relicts dominate and have been preserved to date. Habitat conditions on Tara have not significantly changed since the Tertiary to date. Inversion polymorphism is suitable for this survey since it responds to some extent to climatic change.Gene arrangement frequencies of D. subobscura population from the Derventa river gorge were compared to the ones determined before for the other populations in Serbia. No endemic arrangements were found in the analyzed population, except those characteristic for the Balkan Peninsula refugium, in general. High dispersion capacity of D. subobscura may explain lack of genetic distinctiveness in inversion polymorphism which probably existed in this refugium.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.29

CHROMOSOME NUMBER VARIATION IN SEXUALLY DIMORPHIC SPECIES Mercurialis perennis L. (Euphorbiaceae) FROM SERBIA

Jovanović Vladimir¹, Blagojević Jelena², Vujošević Mladen²and Cvetković Dragana¹

¹Faculty of Biology, Universityof Belgrade, Belgrade, Serbia; ²Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Evolutionary transitions between various sexual systems in plants have attracted a great deal of research. Processes leading to change in chromosome number were important in shaping evolution of genus Mercurialis, that shows exceptional variation in sexual system, as well as extensive chromosomal variation. Previous studies revealed that polyploidy was associated with transition from dioecy to hermaphroditism in M. annua. Its congeneric, M. perennis, is almost exclusively dioecious species with wide latitudinal and altitudinal distribution. It represents the extreme example of chromosome variation – it consists of polyploid and aneuploid complex (2n= 42–112); about 43 different cytotypes have been described so far, most with basic number x=8. While populations from certain parts of European range are well studied in this respect, cytogenetic data from southeastern part is missing. The aim of this study was to examine the variation in chromosome number, i.e. presence of polyploid and aneuploid complex in M. perennis populations in Serbia. Karyological analyses of samples from low altitude populations did not confirm the presence of extreme variation – the observed chromosome numbers fall within the previously reported range, with 2n=60 being the most common variant.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.30

THE EFFECTS OF POLLUTION IN POPULATIONS OF FIELD MOUSE,

Apodemus agrarius – HAEVY METALS AND DEVELOPMENTAL STABILITY

Blagojević Jelena¹, Jojić Šipetić Vida¹, Jovanović Vladimir²and Vujošević Mladen¹

¹Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;²Research station Petnica, Valjevo

Concentration of heavy metals was assessed in bone tissues of widely distributed species of field striped mouse, Apodemus agrarius (Rodentia, Mammalia) from two populations in the locations with different levels of pollution, mountain Cer and Pančevo. Comparison of concentrations of eight heavy metals (Zn, Ni, Co, Mn, Cu, Cd, Pb, Fe) was done between the localities, sex and age categories. The effect of pollution was clear for the most of heavy metals studied. Differences between sexes were found for iron and copper in samples from Pančevo. Bioaccumulation of all heavy metals, except zinc, is increased in the youngest age category on mountain Cer. Using the method of geometric morphometry, the same samples of animals were analysed for the level of fluctuating asymmetry (FA) for mandible and cranium. FA is the measure of developmental stability and is often used for assessing the effects of environmental factors on living systems. Comparison of obtained results and concentrations of heavy metals was done and discussed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.31

THE INFLUENCE OF ELECTROMAGNETIC FIELDS ON FITNESS COMPONENTS DURING DEVELOPMENT OF Drosophila subobscura

Patenković Aleksandra¹, Savić Tatjana¹and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Extremely low frequency (ELF) electromagnetic field (EMF) is increasingly present abiotic environmental factor. Studies of the effect of EMF have been performed on a variety of species from different aspect, but it is still obscure how organisms respond and/or adapt to such environmental change. Electromagnetic field used in this experiment was 0.25mT and of extremely low frequency of 50Hz. This intensity and frequency is common for EMF which are widely used at workplace and home. The effect of exposure to this EMF was observed through fitness components during three generations in species Drosophila subobscura. The females from different inbred lines laid eggs in the presence or absence of EMF for 24 h period, and the progeny is reared in identical conditions for an additional 24 h. Therefore, the progeny was exposed to EMF in embrional and early postembrional stages of development. In both groups, egg-to-adult viability, early fecundity and development time was analyzed. Changes in fitness components during several generations reveal that ELF - EMF is a physical factor wich affects embrional development.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.32

DEVELOPMENTAL INSTABILITY IN Drosophila subobscura IN CONDITIONS OF DIFFERENT DEGREE OF LEAD POLLUTION

Kenig Bojan¹, Kurbalija Zorana¹, Stamenković-Radak Marina^1,2 and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Heavy metal pollution of natural ecosystems is one of the negative consequences of human activity. Lead is one of the most widespread pollutants. Adaptation to toxic substances in the environment can lead to the increased resistance and changes in genetic structure of the exposed populations. Drastic environmental deterioration presents environmental stress which can affect various phenotypic traits, such as developmental homeostasis of organisms. Disturbance of developmental homeostasis, developmental instability (DI), is a suite of processes that tend to disrupt precise development in particular environment. Variation in bilateral traits, such as fluctuating asymmetry (FA), is in a direct correlation with developmental instability, environmental changes and stress. The aim of this study was evaluation of FA level in laboratory populations of D. subobscura exposed to different concentrations of lead. Variation in wing-shape asymmetry in D. subobscura was analyzed by method of geometric morphometrics. In that manner, the relationship between environmental stress caused by lead with DI levels during seven successive generations was analysed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.33

Effect of previous mating experience on some components

of mating behavior in Drosophila melanogaster

Pavković-Lučić Sofija, Kekić Vladimir and Miličić Dragana

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Sexual behavior in Drosophila melanogaster is very complex: it includes series of various courtship steps, followed by an interchange of different types of stimuli, like olfactory, acoustic, visual and tactile. However, many aspects of the reproductive behavior of D. melanogaster may be modified by experience and age. It is well known that in D. melanogaster, females "decide" if and with which male mating will take place. These "decisions" definitely depend on various sensory cues from the males, which they use in demonstration their attractiveness. We supposed that female mating experience also may contribute to mate choice.According to our results, larger males were more succesfull in mating with “experienced” females. Also, females with previous mating experience had significantly slower mating speed than virgin females, while, on the other side, virgin females copulated significantly longer than previously mated ones. Opposite to females, males with or without previous mating experience, did not differ in mean mating speed and mean duration of copulation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.34

Fluctuating asymmetry and sexual selection

in Drosophila melanogaster

Pavković-Lučić Sofija and Kekić Vladimir

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Fluctuating asymmetry (FA) is defined as small, random departures from bilateral symmetry, which is ’’ideal state’’ of bilateral traits; it reflects levels of genomic and environmental stresses during development. Relationship between sexual selection and FA is very complex and, still controversial.In a series of experiments (two in laboratory conditions and one in the field), we investigated if more symmetrical D. melanogaster males were more succesfull in mating. In the laboratory experiment I, males were developed at different growth temperatures (18 ºC vs. 25 ºC), while, in laboratory experiment II, males were reared on one out of three different substrates for more than 35 generations (standard cornmeal medium, banana and tomato).Contrary to results obtained in laboratory, in field experiment, male mating success was associated with low FA. Namely, non-mating males showed elevated FA both in wing length and number of sex comb teeth.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.35

Sexual selection and mating pattern related to body size in three Drosophila species in natural conditions

Kekić Vladimir and Pavković-Lučić Sofija

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Sexual selection is recognized as a special form of natural selection and defined as selection that arises from differences in mating success. Large body size in males emerged as a correlate of mating success across a range of insect taxa, including Drosophila. However, investigations with a number of Drosophila species have indicated that as more Drosophila species were studied, the number of examples where body size and male mating success were uncorrelated was increasing. The goals of the present study were: (i) test for differences in mean body size between mating and non mating (control group) of flies from natural populations of three Drosophila species (D. melanogaster, D. hydei and D. immigrans), (ii) test for mating pattern related to body size. Mating behavior of those species were investigated during two seasons, at two different localities (Sremska Kamenica, Bačko Gradište, Serbia). Body size was correlated with mating success only in D. melanogaster. Size-assortative mating was observed only in D. hydei and only in one season. Different results obtained in these species would be discussed in a course of their different reproductive strategies.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.36

FLUCTUATING ASYMMETRY AND COMPONENTS OF FITNESS IN COMPETITIVE AND NON- COMPETITIVE CONDITIONS IN Drosophila subobscura

Savić Marija¹, Savić Tatjana², Pavković-Lučić Sofija¹, Stamenković-Radak Marina^1,2

and Anđelković Marko^1,2

¹ University of Belgrade, Faculty of Biology, Belgrade, Serbia.

² University of Belgrade, Institute of Biological Research, Belgrade, Serbia.

It has been suggested that fluctua. Some studies failed to report this, but many have reported association between fluctuating asymmetry of bilateral morphological traits and individual fitness traits. This relationship can be explained by sexual selection, if levels of asymmetry influence mate choice, or by natural selection, if increased level of FA are associated with reduced performance for life history traits. We used Drosophila subobscura in the conditions of competitive and non competitive mating, comparing fluctuating asymmetry and components of fitness. The present study focused on the fluctuating variation, of wing size and ovariole number in females and wing size and sex combs number in males, and association with early female fecundity and egg-to-adult viability. The results suggest that individual FA is poor indicator of fitness components and the generality of the relation between developmental instability and fitness remains a controversy. ting asymmetry (FA), as a measure for developmental instability, is associated with fitness

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.37

REPRESENTATION OF SPECIATION OF THE OUTCOME AMONG SPECIALISTS VS. GENERALISTS IN RELATION TO HABITAT IN THE LIMNOFAUNA OF EUROPE

Pavlović Nevenka and Pavlović P. Boro

Faculty of Natural Sciences and Mathematics, Banja Luka

The relations between species and sub-species with types of habitat have been analysed in the frame of the limnofauna of Europe. The register encompasses 15384 species and sub-species, and 53 types of habitat. For 12464 species and sub-species there is information on a linkage with one to nine (1-9) types of habitat grouped in 607 variations, what makes 19038 relations.A scale of the generalists is defined in the span ranging from 0 (zero) for the species using only one type of habitat, to eight (8) for the species appearing in nine (8) types of habitat.The use of the two types of habitat is a transitional phase towards specialisation or increased generalisation in occupation of more types of habitat.High diversity in 53 types of habitat appears in the interval of the scale of generalists ranging from 0.38 to 1.43, while low diversity is present in a larger number of types of habitat in the remaining area of the scale of generalists.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.40

GENERAL OVERVIEW OF THE COMET ASSAY

Anderson Diana

University of Bradford, Bradford, BD7 1DP, UK

The Comet assay has become one of the most important assays in toxicology of the last decade or so. It is now a standard method for measuring DNA strand breaks in single cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt. Electrophoresis at high pHs results in structures resembling comets, observed by fluorescence microscopy. The comet tail is formed by DNA fragments moving towards the anode. The assay can be used for genotoxicity testing of novel compounds and ecotoxins, human biomonitoring and molecular epidemiology and basic research into DNA damage and repair. DNA repair can be measured after treatment of cells with a DNA damaging agent and measuring damage remaining after different time intervals. This overview will be illustrated with examples from the work of the author’s group and collaborators. These will include for genotoxicity testing the specificity and sensitivity of the assay, for ecotoxins the work with DBP halogenated acetic acids, for human biomonitoring and molecular epidemiology the work with mother and babies, diabetes and lead-exposed children and for fundamental research that with drug resistant Non-Hodgkin’s lymphoma patients over-expressing p53 mutant protein and lacking DNA repair. Most of this work has been carried out in somatic human lymphocytes, but it can also be carried out in germ cells as human sperm. Using sperm, the oestrogens can cause DNA damage which can be diminished with anti-oxidants suggesting an oxygen radical involvement. Also as age increases in men so there is an increase in DNA damage.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.41

GENOTOXIC EFFECTS OF ORGANOPHOSPHORUS PESTICIDES

Filipič Metka and Hreljac Irena

National Institute of Biology, Ljubljana, Slovenia

Chronic exposure to organophosphorous compounds (OPs) has been linked to the development of different cancers. With human hepatoma HepG2 cells we showed that methyl-parathion (PT) and methyl-paraoxon (PO) induced DNA damage, whereas dimefox (DF) induced increased cell proliferation. PT and PO upregulated mRNA expression of DNA damage responsive genes: p53, p21, GADD45α and MDM2, while DF upregulated only expression of p53. We further found synergistic effects of PT and PO on BaP induced mutagenesis in the bacterial reverse mutation assay, and micronucleus assay in HepG2 cells. We demonstrated for the first time that the underlying mechanism of synergistic effect in HepG2 cells was PT and PO mediated modulation of the metabolic activation of BaP: OPs suppress activity and expression of cytochrome 1A1 (CYP1A1) and favours the induction of aldo-keto reductase 1C (AKR1C) that catalyse the formation of DNA reactive BaP o-quinones and the production of reactive oxygen species. Our results indicate that different types of OPs affect cancer development by different mechanisms including mitogenic effect, direct genotoxicity and synergistic effects in combination with PAH.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.44

GENETIC MODELS IN INVESTIGATION OF MOLECULAR MECHANISMS OF GENOTOXICITY AND ANTIGENOTOXICITY

Vuković-Gačić Branka, Simić Draga and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

DNA repair mechanisms, which are involved in preserving the integrity of the genome, are largely conserved among prokaryotes and eukaryotes, including human cells. Human XPC protein detects distortion in DNA helix in much the same way as bacterial UvrA, and XPD possess helicase activity similar to bacterial UvrB. Eukaryotic cells also repair mismatches with homologs of MutS and MutL, and genetic predisposition to colon cancer (HNCC) is due to mutations in corresponding MSH and MLH genes. Moreover, human RAD51, a homolog of the bacterial RecA protein, promotes homologous paring and DNA strand exchange. The modulation of DNA repair, and increase or decrease of mutagenic effect, can be monitored in different genetic backgrounds. Genetic endpoints such as spontaneous and induced mutations, recombination, and mutagenic repair can be studied with microbial short-term tests, and addition of mammalian enzymes provides valuable information about genotoxicity or antigenotoxicity in mammals. Our study of antigenotoxic compounds from medicinal and aromatic plants with microbial tests will be presented.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.45

WHAT IS KNOWN AND WHAT IS UNKNOWN IN RADIATION PROTECTION

Joksić Gordana

Institute of nuclear science „Vinča“

This paper summarize contribution of radiobiology in the field of radiation protection in the last 3 decades. The most important discoveries and findings are accounted chronologically and are discussed with respect to protection against of ionizing radiation.

The three decades of intensive research in radiation biology didn’t gave an clear answer on the basic question in biodosimetry- dose-response relationship at low doses of ionizing radiation, in another word a contribution of low doses in neoplastic transformation. Although is known that particular types of ionizing radiation differs significantly in their ability to induce DNA damages, mechanisms underlying this phenomenon are still unclear. Ionizing radiation at doses that are significant for radiation protection behaves as weak carcinogen, whereas irradiation with doses above 500 mSv induce erythema, cataract, infertility. It is known that the incidence of the most primarily induced events gave linear relationship with absorbed dose, which is highly surprising knowing that the multilevel nature of the most biological processes. Although current research didn’t gave a final answers to many questions, it is obvious that ionizing radiation induce complex interactions among many molecules and processes in cells Novel methods, developed for studying of DNA are of importance for radiation protection, and will be discussed in this paper.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.48

ANTIOXIDANTS REDUCE DNA DAMAGE INDUCED BY ADRENALINE

IN THE COMET ASSAY

Radaković Milena¹, Đelić Ninoslav¹and Plećaš-Solarović Bosiljka²,

Spremo-Potparević Biljana², Živković Lada²and Bajić Vladan³

¹Department for biology, Faculty of veterinary medicine, University of Belgrade

²Institute for physiology, Faculty of pharmacy, University of Belgrade

³Institute for research and development, Department for biomedical research, Galenika, Belgrade, Serbia

Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to covalent damage of cellular macromolecules including DNA. In this work we investigated level of primary DNA damage in isolated human lymphocytes exposed to adrenaline. DNA damage was evaluated by the in vitro Comet assay (single cell gel electrophoresis). Firstly, we examined dose-response relationship after the treatment with a broad spectrum of adrenaline concentrations (range from 0.0005 μM to 500 μM) and we found out that with the increase of the adrenaline dose there is an increase of DNA migration. At the concentration of 500 μM we observed the most profound genotoxic effects and acceptable level of cytotoxicity (less than 25%, determined by Trypan blue exclusion test). Therefore, we chose this concentration for further examination with antioxidants catalase and quercetin. It has been observed that catalase (100 IU and 500 IU) and quercetin (100 μM and 500 μM) significantly reduced quanitity of DNA in comet tails. Therefore, it can be concluded that adrenalin exerts genotoxic effects mainly through induction of reactive oxygen species (ROS).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.49

GENOTOXIC PROPERTIES OF ELECTRIC ARC FURNACE DUST ON HUMAN LYMPHOCYTES: IN VITRO STUDY

Garaj-Vrhovac Vera and Gajski Goran

Institute for Medical Research and Occupational Health, Mutagenesis Unit, Zagreb, Croatia

One of the global problems currently recognised worldwide, is a rising exposure of general population to small amounts of leachates of electric arc furnace dust (EAFD), which can pollute large volumes of ground and surface waters.The purpose of this work was to test the alkaline comet assay as a biomarker of the genotoxicity of EAFD leachates, established prior/after the alkaline extraction process. Prior and following the treatment, lymphocytes were incubated with leachate of EAFD for 6 and 24 hours. Results showed that EAFD leachates induced comet assay-detectable DNA damage in human lymphocytes compared to the control. The same goes for the samples exposed to EAFD leacheates pre-treated with a strong alkaline solution. Our data indicate that the ever-increasing amounts of mixture of a number of toxic chemicals, originated from various waste materials residual to a variety of technological processes, such as EAFD, may be responsible for the DNA damage, and suggest that the exposure of human population to such leachates may lead to adverse healthy effects. In addition, comet assay prove to be sensitive when assessing genotoxicity of various waste materials, such as EAFD leachates.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.50

CHEMICAL, CYTOTOXIC AND GENOTOXIC PROPERTIES OF WASTEWATER FROM METAL INDUSTRY

Durgo Ksenija^a, Kopjar Nevenka^b, Franekić Čolić Jasna^a, Šulić Krsta^c, Ćurčić Marijana^d and Antonijević Biljana^d

^a Faculty of Food Technology and Biotechnology, Zagreb, Croatia;^b Institute for Medical Research and Occupational Health, Zagreb, Croatia;^c Public Health Institute, Požarevac, Serbia;^d Institute of Toxicology academic Danilo Soldatović, Faculty of Pharmacy, Belgrade, Serbia

In many countries there is a growing problem concerning environmental pollution from industrial wastewaters. Majority of wastewaters that are released into environment satisfy law regulative on limit values of hazardous substances in wastewater, but mixture of compounds can cause deleterious effects on biological system. In this study we investigated chemical composition and genotoxic effects of wastewater collected from five locations of the region of metal industry. For determination of chemical composition of wastewater we used the method of atomic absorption spectrophotometry (AAS). Mutagenic potential of wastewater was determined on Salmonella typhimurium strains. Cytotoxic effect and primary DNA damage of wastewater was measured on human laryngeal and cervical carcinoma cells. Compounds present in five wastewater samples do not exceed permissible limits. Nevertheless, higher concentrations of wastewaters caused cytotoxic effect on both cell lines, but HeLa cell line was more responsive to lower concentrations of wastewater. All samples caused primary DNA damage in dose-responsive manner. Based on results obtained, additional treatment of the wastewater prior to discharge is recommended.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.52

INFLUENCE OF ANTIOXIDANT ENZYMES ON THE RADIOBIOLOGICAL RESPONSE OF FANCONI ANEMIA LYMPHOCYTES IN VITRO

Petrović Sandra¹, Vujić Dragana², Guć-Šćekić Marija², Joksić Ivana³,

Leskovac Andreja¹, Joksić Gordana¹

¹Institute of nuclear science „Vinča“;²Mother and Child Health Care Institute of Serbia “Dr. Vukan Čupić“, Belgrade;³Gynecology and Obstetrics Clinic „Narodni front“, Belgrade, Serbia

Fanconi anemia (FA) is genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a redox abnormalities indicating that FA is oxidative stress related disorder. One of the features of FA cells that remain unclear is their response to ionizing radiation. The aim of this study was to evaluate the in vitro radiosensitivity of FA homozygotes and heterozygotes, and to assess the contribution of antioxidant enzymes to the overall radiobiological response of the cells. FA patients exhibited a significant decrease in catalase activity, accompanied with lower activity of SOD and increased incidence of baseline micronuclei compared to control. The most of patients displayed a radioresistant response accompanied with enhanced percentage of radiation-induced apoptosis. Activity of catalase and percentage of apoptotic cells correlated inversely. Enhanced apoptosis also was observed in heterozygote-parents and was accompanied with lower activity of catalase in carrier-mothers. The results of study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.53

RADIOSENSITIVITY OF FANCONI ANEMIA FIBROBLASTS IN VITRO

Leskovac Andreja¹, Petrović Sandra¹, Joksić Ivana², Vujić Dragana³,Guć-Šćekić Marija³,

Slijepčević Predrag⁴and Joksić Gordana¹

¹ Institute of nuclear science „Vinča“;² Gynecology and Obstetrics Clinic „Narodni front“, Belgrade, Serbia;³Mother and Child Health Care Institute of Serbia “Dr. Vukan Čupić“, Belgrade;⁴Brunel University West London, Uxbridge, UK

Fanconi cells are highly sensitive to DNA crosslinking agents, but their response to ionizing radiation is still unclear. This aimed us to investigate in vitro cellular radiosensitivity of aplastic (AA) and Fanconi (FA) anemia fibroblasts employing enumeration of γH2AX foci and determination of apoptotic cells percentage. Foci of γH2AX, detected by immunofluorescence, are used for quantitative analysis of DSBs. The maximum of γH2AX foci in FA cells was 30 min, whereas in AA cells was 24 h after irradiation. In control cells apoptosis reached the maximum value 30 min and 24 h after irradiation. In both FA and AA cells maximum of cells dying via apoptosis were 2 and 24 h after irradiation. Results of the present study indicate disturbance of radioresponses of FA and AA cells seen as significant delay in apoptosis and cell cycle arrest. Furthermore, radioresponses 30 min after irradiation could be significant parameter for distinguishing between FA and AA cellular phenotypes. This study has shown that γH2AX foci represent very sensitive assay for estimation of intrinsic genome radiosensitivity, and demonstrate clear difference in behaviour of FA, AA and normal cells in response to ionizing radiation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.54

COMPARATIVE ANALYSIS OF PREMATURE CENTROMERE DIVISION AND MICRONUCLEI IN PERSONS PROFESSIONALLY EXPOSED TO RADIONUCLIDES

Rakić Boban¹, Jovičić Dubravka², Vukov Tanja³, Pajić Jelena¹, Kovačević Radomir¹, Stevanović Milena⁴and Drakulić Danijela⁴

¹Institute of Occupational Medicine and Radiological Health “Dr Dragomir Karajović”, Belgrade;²The Faculty of Applied Ecology FUTURA, Singidunum university, Belgrade;

³Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;⁴ IMGGI – Institute for molecula rbiology and genetic engeenering, Belgrade, Serbia

The aim of this paper was the comparative analysis of micronuclei (MN) and premature centromere division (PCD) in lymphocytes of peripheral blood in persons exposed to radionuclides. Two groups of patients were used for above mentioned analyses: 50 patients exposed to radionuclides (average age 45.24 ± 1.18, average exposition time 17.96 ± 1.15 years) and 30 control patients (average age 36.07 ± 1.11, average years of employment 10.77 ± 1.07 years). Our data showed that patients exposed to radionuclides had statistically significant higher frequencies of PCD and MN (Mann Whitney U Test, p<0.01). The sex and smoking have no influence on frequency of analyzed parameters. Also, there is significant positive correlation in frequencies of tPCD (PCD present in more then 10 chromosomes per metaphase) and MN. Using FISH method with probe pL1.84 repetitive DNA for chromosome 18 confirmed presence of PCD in interphase nuclei and metaphases. Taking into account that PCD was confirmed in persons with previously detected higher frequency of MN, our research suggests that PCD might be used as potential useful cytogenetic marker in persons professionally exposed to radionuclides.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.55

LOW DOSE GENOTOXICITY OF 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide IN MICE BONE MARROW CELLS

Kalcheva Petrova Vanya, Dragoeva Pencheva Asya, Kalchev Nikolov Karamfil and Enchev Dimitrov Dobromir

Faculty of Natural Sciences, University of Shumen, Shumen, Bulgaria

The chemistry of organophosphorus compounds is a subject of increasing interest, especially the heterocyclic compounds. There are data that some known organophosphorus compounds are mutagens. Although humans are chronically exposed to most environmental chemicals at low doses, genotoxicity assays with rodents are usually performed at high doses. The objective of this investigation was to determine genotoxicity of low doses (2.82x10^–6 μg/kg and 2.82x10^–9 μg/kg) of 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide (Br–oxph) on ICR mice bone marrow cells. Br–oxph induced chromosome aberrations in bone marrow cells after treatment for 3 h and during 24 h and 48 h recovery. The induction of chromosome aberrations demonstrates that the Br–oxph has a genotoxic effect at tested doses.

Acknowledgements: This work was supported by the Bulgarian Ministry of Education and Science, grant no. DO02-86/13.12.2008

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.56

LOW DOSE GENOTOXICITY OF 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide in Allium cepa L. ROOT TIP CELLS

Kalcheva Petrova Vanya, Dragoeva Pencheva Asya, Kalchev Nikolov Karamfil

and Enchev Dimitrov Dobromir

Faculty of Natural Sciences, University of Shumen, Shumen, Bulgaria

To provide a broad coverage of the mutagenic potential of a chemical, information on different experimental test-systems is required. In preliminary study we observed genotoxic effect of low doses of 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide (Br–oxph) in ICR mice bone marrow cells. The objective of the present study was to determine genotoxicity of Br–oxph at low doses (10^–12М and 10^–15М) using Allium cepa L. - test. Treatment with Br–oxph for 3 h produced alterations in the mitotic index and increased the frequency of chromosome abnormalities in Allium cepa root tip cells. These effects remained 48 h after the treatment. The data from the study showed the existence of cytotoxic and genotoxic effects of Br–oxph.

Acknowledgements: This work was supported by the Bulgarian Ministry of Education and Science, grant no. DO02-86/13.12.2008

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.57

ARE PET BOTTLES FOR MINERAL WATERS GENOTOXIC?

– TESTING WITH Allium Test

Stefanović Marina, Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Polyethylene terephthalate (PET) is relatively new material, which is widespread in practice for packaged food and mineral water. This material, characterized by thermostability, shatter resistance, lightweight, and transparency, has replaced PVC wrapping material. In the manufacture of PET, Sb₂O₃ is used as the catalyst and it was found that Sb can be realised in the bottled water. Potential genotoxic effects of two brand mineral waters from Serbia were analysed by Allium anphase-telophase test. After opening, the bottles with mineral waters were kept at three different temperatures, +4^oC, +50^oC and +60^oC, respectively. The results show that the warmed water samples significantly reduce onion growth and root length growth. Heating increases toxicity of mineral waters. On the other hand, the way the water is kept does not affect significantly the level of genotoxicity. This parameter is defined by frequency of aberrant cells, i.e. between 7.1% and 12.0%.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.58

EVALUATION OF GENOTOXIC EFFECT OF TOCOLYTIC RITODRINE, ANTIARRHYTHMIC VERAPAMIL AND ANTIBIOTIC ERYTHROMYCIN ON THE MICRONUCLEI FREQUENCY IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES

Grujičić Darko¹,Milošević-Djordjević Olivera^1,2 and Stošić Ivana¹,

Arsenijević Slobodan² and Marinković Dragoslav³

¹Faculty of Natural Science, Kragujevac;²Faculty of Medicine, Kragujevac

³ Serbian academy of science, Belgrade

The aim of this study was to investigate the genotoxic effect of ritodrine, verapamil and erythromycin on human peripheral lymphocytes in vivo and in vitro, by application of cytokinesis block micronucleus (CBMN) test. In the sample of 32 patients, the average of MN frequency was significantly higher after combined therapy in comparison to the MN frequency in the same patients before therapy (p<0.001). In order to assess single effects of medicaments in the cultures of peripheral lymphocytes effects of different concentrations, for ritodrine 8,4x10^-6M-25,2x10^-4M, verapamil 0,56-11x10^-5M and erythromycin 0,68-5,45x10^-4M were tested. The increase of concentrations of ritodrine and verapamil increases the MN frequency. In the treatment with concentrations equivalent to daily therapeutical doses (DTD), both drugs single or combined induced statistically significant increase of MN (p<0.05; p<0.001). Erythromycin in tested concentrations did not change MN frequency (p>0.05). The obtained results show that ritodrine and verapamil in vivo and in vitro have significant genotoxic and co-mutagen effect.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.59

EVALUATION OF THE DNA DAMAGE LEVEL IN HUMAN LYMPHOCYTES EXPOSED TO EPHEDRINE IN VITRO

Đelić Ninoslav¹, Radaković Milena, Spremo-Potparević Biljana², Plećaš-Solarović Bosiljka²_,Živković Lada² and Bajić Vladan³

¹Department for biology, Faculty of veterinary medicine, University of Belgrade;²Institute for physiology, Faculty of pharmacy, University of Belgrade;³Institute for research and development, Department for biomedical research, Galenika, Belgrade, Serbia

Ephedrine is a sympathicomimetic isolated from the plant Ephedra vulgaris. It has similar chemical structure to adrenaline and pharmacodynamic action similar to catecholamines. Ephedrine acts mainly through release of catecholamines from their depots, but it also stimulates adrenergic receptors. Although ephedrine has not exhibited genotoxic effects in some test systems, to our knowledge it has not been tested in the Comet assay. Therefore, the objective of this study was to evaluate whether ephedrine can induce primary DNA damage in isolated human lymphocytes using in vitro Comet assay. Firstly, we examined a broad spectrum of ephedrine concentrations, in a range from 0.0005 μM to 500 μM. We found out that ephedrine does not cause significant departures from the negative control level (the solvent). Three concentrations (1, 50 and 300 μM) with acceptable cell viability (over 75%) were further tested with inhibitors of DNA repair (cytosine arabinoside and hydroxyurea) and we did not observe any significant changes compared with cultures without DNA repair inhibitors. It can be concluded that ephedrine does not exhibit genotoxic effects in the in vitro Comet assay on human lymphocytes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.60

Genotoxic and citotoxic effects of fumagillin

Stanimirović Zoran and Stevanović Jevrosima

¹Department for biology, Faculty of veterinary medicine, University of Belgrade

Fumagillin, an antibiotic produced by Aspergillus fumigatus is used against microsporidiosis of bees and fish. In humans, fumagillin is suggested for the treatment of chronic microsporidial diarrhea and keratoconjunctivitis in patients with AIDS. Genotoxic effects of fumagillin were evaluated in vitro and in vivo. All tested concentrations of fumagillin (0.34, 0.68, 1.02, 3.07 and 9.20 μg/ml) applied to lymphocyte cultures increased the frequency of SCE significantly (p<0.001 or p<0.01). The three highest concentrations induced a significant (p<0.001) increase in structural CA, significant increase in MN formation and significant decrease in MI, PI, and NDI. In in vivo study BALB/c mice were treated with three doses of fumagillin (25, 50 and 75 mg/kg bw). All tested doses induced a significant (p<0.001) decrease in MI and a significant (p<0.001) increase in the frequency of MN. Significantly increased frequencies (p<0.01 or p<0.001) of numerical and structural CA were observed at 75 mg/kg bw. A significant (p<0.001) increase in the frequency of Rb(4.19) was observed at 50 and 75 mg/kg bw. These results confirm the genotoxic potential of fumagillin as well as its cytotoxic activity.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.61

GENOTOXICITY TESTING OF EFFECTS OF NATURAL AGENTS

Stanić Snežana¹, Matić Sanja¹ and Solujić Slavica²

¹Department of Biology, Faculty of Science, University of Kragujevac,

Kragujevac;²Department of Chemistry, Faculty of Science, University of Kragujevac,Kragujevac, Serbia

The genotoxicity effects of the acetone and watery extracts of plant Ambrosia artemisiifolia L. (Anacardiaceae) pollen and methanolic extract of plant Cotinus coggygria Scop. (Anacardiaceae) stem were investigated in vivo conditions using the sex-linked recessive lethal test (or SLRL test) on a eukaryotic model system Drosophila melanogaster.With applied experimental procedure we were able to quantify a mutegenic potential of agents of natural origin: two lactones, ambrosin and dihydroambrosin were identified in the acetone extract of Ambrosia artemisiifolia pollen, and pyrocatechol from the methanol extract of C. coggygria stem.The obtained results showed significant increases in the frequency of sex-linked recessive lethal mutations in premeiotic and postmeiotic germ cell lines of the tested males of species Drosophila melanogaster.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.62

EXAMINATION OF DIFFERENT RASPBERRY LEAVES (Rubus idaeus L.) METHANOL EXSTRACTS ON HELA CELLS VIABILITY AND PROLIFERATION

Vukelić Marija¹, Kitić Dušanka², Pavlović Dragana², Živanov-Čurlis Jelena¹,

Živković Jelena¹and Najman Stevo¹

¹Faculty of medicine, Institute for biomedical research, Niš

² Faculty of medicine, Integrated academic study of pharmacy, Niš, Serbia

The results of various phytochemical investigations show that raspberry leaves (Rubus idaeus L.) is rich source of flavonoids, ellagic acid and tannins. Experiments proved that the individual compounds belonging to these groups have antioxidant, antimicrobial, anti-inflammatory, antiviral and anticarcinogen activity. The aim of our study was examination of different raspberry leaves methanol exstracts on HeLa S3 cells viability and proliferation. Different methanol exstracts were prepaired by maceration in absolute methanol, ultrasount extraction in absolute methanol, maceration in 70% methanol and ultrasount extraction in 70% methanol. Efective exstracts concentrations were 0.0001mg/ml, 0.001mg/ml, 0.01mg/ml, 0.1mg/ml, 1mg/ml.Cell viability and proliferation was examined due to tetrazolium salt reduction ability (MTT test) and HeLa cells morphological characteristics after 24h and 72h incubation with extracts.The results of this investigation show that all investigated methanol extracts in these concentrations showed a mild impact on the viability of treated HeLa cells in comparison to the control group.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.63

ANTIGENOTOXIC PROPERTY OF AQUEOUS EXTRACT OF MEDICINAL MUSHROOM Agaricus blazei Murrill, ON THE GENOTOXICITY INDUCED BY MMS

Savić Tatjana¹, Patenković Aleksandra¹, Soković Marina¹, Glamočlija Jasmina¹, Radulović Višnja and Anđelković Marko^1,2

¹ Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Agaricus blazei Murrill, popularly known as the „sun mushroom“, is a native mushroom in Brazil, that has been widely used in folk medicine mainly in the form of tea to treat various ailments (diabetes, hypercholesterolamia, osteoporosis, gastric ulcer, stress, etc.), but mostly for prevention and treatment of cancer.The wing Somatic Mutation And Recombination Test (SMART) in Drosophila melanogaster was used to study the antigenotoxic effect of mushroom Agaricus blazei Murrill, in combination with the alkylating agent methyl methanesulfonate (MMS). Two-day-old larvae, trans-heterozygous for recesive markers multiple wing hairs (mvh) and flare-3 [flr (3)], were fed for 24h with aqueous extract of Agaricus blazei. For antigenotoxicity studies separate 24h pre-treatment with aqueous extract of Agaricus blazei, followed by chronic treatment with a MMS, was done. Genetic changes induced in somatic cells of the wing imaginal discs lead to the formation of mutant clones on the wing blade. Our results showed that aqeous extract of Agaricus blazei can possess antigenotoxic and/or protective effect against the activity of mutagenic agent.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.64

ANTIMUTAGENIC POTENTIAL OF CAMPHOR, α/β THUJONE AND EUCALYPTOL IN THE BACTERIAL ASSAY SYSTEM

Nikolić Biljana, Milojević Nataša, Janković Ivana, Kolarević Stoimir, Jovanović Predrag, Jagodić Aleksandra, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

We have tested bioantimutagenic potential of monoterpenes Camphor (C), α/β Thujone (T) and Eucalyptol (E) and elucidated molecular mechanisms of mutagenesis using E. coli K12 assay system.The inhibition of UV- and 4NQO-induced mutagenesis was monitored by reversion test in repair proficient and NER deficient strains. All tested monoterpenes significantly reduced induced mutagenesis only in repair proficient strain, confirming the involvement of NER in bioantimutagenic effect. The effect of monoterpenes on the SOS induction kinetics following UV-irradiation was studied by measuring activity of b-galactosidase, expressed from sfiA::lacZ fussion. The effect on protein synthesis was determined by measuring activity of constitutive alkaline phosphatase. The growth rate, protein syntesis and SOS induction were differently affected by monoterpenes. T reduced growth rate, protein syntesis and the level of b-gal, with no effect on enzyme induction kinetics. E and C reduced the rate of b-gal induction, but C increased while E decreased the enzyme level, compared with the control. Obtained results indicate different mechanisms of increased efficiency of NER in the presence of monoterpenes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.65

THE EFFECT OF RAT LIVER ENZYMES ON ANTIMUTAGENIC POTENTIAL OF MONOTERPENES AGAINST t-BOOH-INDUCED MUTAGENESIS IN E. coli

Mitić-Ćulafić Dragana, Janković Milena, Ćakić Sanja, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Oxidative DNA damage is one of the most common threats to genome stability and may contribute to carcinogenesis, diabetes and ageing by inducing oxidative stress. In addition to cellular antioxidative defense mechanisms and DNA repair enzymes, some natural compounds can act as protective substances against reactive oxidative species (ROS) induced DNA damage. The aim of this work was to investigate the protective capacity of monoterpenes: Linalool (L), Eucalyptol (E) and Myrcene (M) against t-BOOH-induced oxidative mutagenesis in E. coli. The WP2 reversion assay was performed with strain IC202 oxyR, deficient in induction of antioxidative enzymes, with or without S9 fraction. The fraction contains rat liver cells enzymes involved in metabolic transformation and detoxification of xenobiotics.The obtained results showed that L, E and M possess high protective capacity against t-BOOH-induced mutagenesis. In the presence of S9 enzymes antimutagenic potential of tested monoterpenes was reduced, suggesting their processing to less protective metabolic forms. Obtained results are possibly relevant for the development of dietary or pharmaceutical cancer chemopreventive supplements.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.66

EVALUATION OF ANTIGENOTOXIC POTENTIAL OF LINALOOL, EUCALYPTOL AND MYRCENE AGAINST IQ- AND PhIP-INDUCED DNA DAMAGE

Mitić-Ćulafić Dragana¹, Žegura Bojana², Knežević-Vukčević Jelena¹ and Filipič Metka²

¹Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade

²National Institute of Biology, Ljubljana, Slovenia

It has been reported that many plant components reduce the genotoxic effect of carcinogens, including heterocyclic amines. Monoterpenes: Linalool (L), Eucalyptol (E) and Myrcene (M) are strong antioxidants and free radical scavengers present in many medicinal and aromatic plants. In our previous work we have shown that L, E and M efficiently prevented t-BOOH- induced DNA damage and mutagenesis in bacteria and cultured human cells. In this work we studied antigenotoxic potential of L, E and M against food borne heterocyclic amines IQ and PhIP in human hepatoma HepG2 cells using Comet assay. The range of non-toxic concentrations of L, E and M was determined with MTT test. The cells were exposed to IQ or PhIP for 21h with or without L, E or M and subjected to the alkaline Comet procedure. The DNA damage induced by PhIP was significantly reduced (40-63%) by each of the tested substances, while inhibition of IQ-induced DNA damage was less efficient. Considering that PhIP is known to induce reactive oxygen species, we propose that more efficient protection against PhIP-induced genotoxicity is due to antioxidative activity of the tested substances.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.67

Antigenotoxic potential of tea infusion of Gentiana lutea in wing spot test OF Drosophila melanogaster

Patenković Aleksandra¹, Stamenković-Radak Marina^1,2, Nikolić Dragana, Marković Tamara and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković, Belgrade

² Faculty of Biology, University of Belgrade, Belgrade

The root of Gentiana lutea is an ingredient of many proprietary medicines, which has a long history of use as an herbal bitter in the treatment of digestive and numerous other disorders. Gentian root possesses therapeutic effects due to the presence of bitter glycosides, with main components gentiopicrin, gentiamarin and swertiamarin.

The antigenotoxic action of gentian was evaluated by the wing Somatic Mutation and Recombination Test (SMART) on D. melanogaster. This test provides sensitive, rapid and reliable verification of mutational and recombinational potential of various chemicals and their antigenotoxic property. Two mutagens, methyl methanesulfonate as an alkylating agent and hydrogen peroxide as an oxidative reagent, were used to evaluate the antigenotoxicity of gentian. Third-instar larvae, trans-heterozygous for two genetic markers, multiple wing hairs (mwh) and flare (flr), were fed mutagens alone and in combination with tea infusion of gentian. Results reveal the difference in antimutagenic effects of gentian infusion. Usage of mutagens with different mechanisms of affecting DNA molecule and different treatments explain mechanisms of antimutagenic effect of G. lutea.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.70

HEDGEHOG SIGNALING PATHWAY IN DEVELOPMENT AND TUMOR FORMATION

Milenković Ljiljana

Stanford University Medical Center, Stanford, USA

Hedgehog (Hh) signaling pathway, first identified in a genetic screen for genes that control embryonic development in the fruit fly, regulates cell proliferation and differentiation in many tissues and organs, not only in flies, but also in vertebrates, including humans. Mutations in the genes components of the pathway can lead to birth defects and cancer. Inappropriate activation of the pathway has been implicated in several types of cancer, including medulloblastoma, basal cell carcinoma, rhabdomyosarcoma and others. That is one of the reasons we would like to fully understand how this signaling pathway works and find ways to modify it, in cases when it doesn't function properly. An interesting insight into Hh signaling in vertebrates came several years ago from genetic evidence in mice that suggests that the primary cilium, a cell organelle present in most cells in vertebrates, has an important role in processing of the Hh signal. We are using genetic, cell biological and biochemical approaches to further understand the role of cilia as a center for receiving and transmitting the Hh signal. Recent advances towards this goal will be presented.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.72

POLYMORPHISM C677T IN THE GENE ENCODING FOR METHYLENETETRAHYDROFOLATE REDUCTASE

IN DIFFERENT VASCULAR DISORDERS

Novaković Ivana¹, Damnjanović T.¹, Sango Violeta², Mladenović O.¹, Maksimović N.¹, Mirković Duško³, Antonijević Nebojša⁴, Simić-Ogrizović S.⁵, Sternic N.⁶ and Luković Ljiljana¹

¹Institute of Human Genetics, School of Medicine, Belgrade, ²Laboratory of Medical Genetics, HC Jagodina, Jagodina, ³Institute of Medical Biochemistry, ⁴Institute of Cardiovascular Diseases, ⁵Institute of Nephrology and Urology, ⁶Institute of Neurology, CCS, Belgrade, Serbia

It is well documented that higher plasma level of the amino acid homocysteine has atherogenic and prothrombotic action. For these reasons homocysteine metabolism is studied in large number of phisiological and pathological conditions. Activity of the enzyme methylene-tetrahydrofolate reductase (MTHFR) is one of the key points of homocysteine metabolism, involved in the remethylation of homocysteine to methionine. Gene polymorphism MTHFR C677T reduced the enzyme activity and is associated with risk of the hyperhomocystenemia. During the last five years we analyzed MTHFR C677T polymorphism and ists association with vascular disorders in different groups. In whole population established frequency of 677T allele and TT genotype was about 0.40 and about 15%, respectively, but data varied with age. Our studies showed association of T allele and TT genotype with higher lipid level and higher artherial tension in healthy school-aged child, as well as in young patients with stroke and in hemodyalisis patients. On the contrary, no significant association observed for 677 T allele and TT genotype in young patients with myocardial infarction and with pulmonary embolism.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.74

CYTOGENETICS-THE PRESENT, THE FUTURE AND PERSPECTIVE

Milićević Radomir, Branković Ljiljana, Radulović D., Jugović D., Stanković T., Stamenković H., and Kamenov B.

Clinical Center Nis, Clinical for child internal diseses, Laboratory for Immunology and Genetics, Nis, Serbia

Chromosome aberration frequency in the general population is approximately 0,6% though they occur more often ih high risk groups. The risk group mainly consists of pregnant women above 35 years, young pregnant women who have underwent alternative diagnostic methods wich raised doubts about their condition, couples with primary sterility etc.We present in this study the results of our laboratory for the year 2008. We analysed karyotype of pheripheral blood, bone merrow and amniotic cells after cultivation in RPMI or Quantum medium. All specimen were G-banded using trypsin- Giemsa stain. A total of 961 karyotypes has been analyzed: 311 karyotypes obtained from blood counted 33 abnormal cases, 481 from amniotic had 11 aberrant cases and 179 karyotypes obtained from bone marrow had 37 aberrant metaphases.Cytogenetic is a method with significant and irreplaceble role in the discovery of chromosomal aberrations. Detection of chromosomal abnormalities provides the opportunity to plan further treatment of reproduction disorders.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.75

TYPES OF bcr-abl REARRANGEMENTS IN SERBIAN POPULATION AND A LEVEL OF EXPRESSION IN CHRONIC MYELOID LEUKEMIA PATIENTS

Todorić-Živanović Biljana^a, Strnad Milica^a, Stamatović Dragana^a, Grujović Jelena^b,

Tanić Nikola^c, Ilić Vesna^aand Magić Zvonko^a

Military Medical Academy^a, Applied biosystems^b, Institute for Biological research^c, Belgrade, Serbia

Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of a pluripotent chematopoetic stem cell. In more than 95% of cases, it is characterized by the presence of chimeric bcr-abl gene which is formed by the translocation t(9;22)(q34;q11) or by other molecular mechanisms. Although well known by it’s clinical features it is molecularly heterogenic disease. The Bcr-abl, p210kD, protein with its elevated tyrosine kinase activity has a central role in pathogenesis of CML. Those knowledge gave rise for developing molecularly targeted therapy for CML with tyrosine kinase inhibitors. In this study we investigated types of bcr-abl rearrangements in CML patients (pts) in Serbian population. We analyzed 112 patients by RT-PCR method. Level of bcr-abl expression was quantified by the absolute real-time PCR (RQ-PCR) method in 20 treated pts. The level of expression in 15 de novo, untreated, pts was measured too, for constructing base line. In 85 (76.9%) pts, b3a2 form of bcr-abl gene was detected and b2a2 form in 26 (23.2%). One pt (0.9%) was bcr-abl negative, bath with e1a2 form of bcr-abl in blastic transformation of the disease. The level of expression of treated pts was compared with base line.Predomination of b3a2 form of bcr-abl gene, in our population, is in correlation with literature data for European population.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.76

MOLECULAR DIAGNOSTIC OF CHILDHOOD ALL

Lakić Nina¹, Krstić Aleksandra¹, Guć-Šćekić Marija¹, Kuzmanović Miloš², Mićić Dragan², Janjić Dragana³, Konstantinidis Nada⁴and Kostić Gordana⁵

¹Laboratory for Medical Genetics, Mother and Child Healthcare Institute, Belgrade; ²Department for Hematological Oncology, Mother and Child Healthcare Institute, Belgrade; ³Department for Hematological Oncology University Children's Hospital in Belgrade;⁴Center for Hematological Oncology Institute for health care of Children and Youth of Vojvodina, Novi Sad;⁵Department for Hematological OncologyChildren hospital, Niš

According to the BFM protocol for treatment of childhood ALL, molecular genetic analyses are a part of routine diagnostic procedure. They play an important role in risk group classification, choice of therapy and detection of MRD during follow-up. After several large studies it had been shown that the presence of fusion genes BCR/ABL, TEL/AML1, MLL/AF4 i PBX1/E2A in transformed blood cells are specific for ALL in children. Developing of RT-PCR technique allow us the detection of fusion gene transcripts of these four the most frequent rearrangements in ALL with high target sensitivity of 10 ^-6cells.RT-PCR analyses for detection of the genetic rearrangements mentioned above have been performing at the Laboratory of Medical Genetics, Mother and Child Health Care Institute, Serbia according to standard BIOMED protocol since May 2007.A total of 87 patients with ALL were analysed using RT-PCR method, during the diagnosing procedure. The BCR/ABL fusion gene (p190 type) was detected in four children (≈5%), equal to the presence of PBX1/E2A rearangement (≈5%). Hibride gene MLL/AF4 was seen in two patients (≈2%) and the most frequent was TEL/AML1 rearangement detected in 19 (≈22%) patients.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.77

EKSPRESSION OF BCL2 PROTEIN AND AMPLIFICATION OF C-MYC GENE IN PATIENTS WITH CHRONIC MYELOID LEUKEMIA

Strnad Milica, Brajušković Goran, Strelić Nataša, Todorić-Živanović Biljana, Stamatović Dragana and Magić Zvonko

Military Medical Academy, Belgrade, Serbia

Chronic myeloid leukemia (CML) represents the malignant myeloproliferative disease developed out of pluripotent hematopoietic stem cell that contains fusion bcr-abl gene. Disorders that occur in the process of apoptosis represent one of the possible molecular mechanisms that bring about disease progress. Within our study we carried out the analysis of the presence the amplification of c-myc oncogene as well as the analysis of the changes in expression of Bcl-2 in patients with CML. Our study included 25 patients with CML (18 in chronic phase, 7 in blast transformation). Using immunohistochemical APAAP method we analyzed the expression of cell death protein in mononuclear bone marrow cells of 25 CML patients. By differential PCR method we followed the presence of amplified c-myc gene in mononuclear peripheral blood cells. The level of expression of Bcl-2 protein is considerably higher in bone marrow samples of patients undergoing blast transformation of disease. The amplification of c-myc gene has been detected in 30% patients in blast transformation of disease. The results of this work have shown that expression of Bcl-2 protein and amplification of c-myc gene are in correlation with disease progression.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.78

RESULTS OF CYTOGENETIC AND MOLECULAR TESTING IN SERBIAN PATIENTS WITH NIJMEGEN BREAKAGE SYNDROME

Ćirković Sanja¹, Guć-Šćekić Marija¹, Pašić Srđan², Radivojević Danijela¹and Jovanović Ankica³

¹Laboratory for Medical Genetics, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade;²Department for Imunology, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade, ³Department for Hematological Oncology, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade, Serbia

Nijmegen breakage syndrome (NBS) is a rare autosomal recessive disorder, characterized by microcephaly, facial dysmorphism, immunodeficiency and cancer susceptibility, with increased cellular hypersensitivity to specific mutagenic agents such as bleomycin(BLE). Molecular diagnosis of NBS is based on screening for the presence of mutations in NBN gene, especially 5-bp deletion(657del5) in exon 6, the most frequent mutation in Slavic population.We investigated five patients with NBS, diagnosed and treated at the Mother and Child Health Care Institute of Serbia (2005-2007). Cytogenetic testing was carried out on peripheral blood cultures using standard procedure.Only three of four blood cultures were successful and exhibited chromosome breakage BLE-induced increases (0.27-0.81breaks/cell) and aberrations including chromosomes 7 and 14.Molecular analysis for the presence of 657del5 mutation in NBN gene was carried on DNA samples from peripheral blood, using modified PCR method on PAGE gel.Homozygosity has confirmed for all (five) tested patients. Early and precise diagnosis of NBS is very important for the management of the disease and for genetic counseling of the families at risk.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.79

PROGNOSTIC SIGNIFICANCE OF TUMOR SUPPRESSOR GENES HYPERMETHYLATION IN ORAL SQUAMOUS CELL CARCINOMA

Šupić Gordana¹, Kozomara Ružica², Branković-Magić Mirjana³, Jović Nebojša²

and Magić Zvonko ¹

¹Institute for Medical Research, Military Medical Academy, Belgrade, ²Clinic for Maxilofacial Surgery, Military Medical Academy, Belgrade, ³Institute for Oncology and Radiology, Belgrade, Serbia

Oral squamous cell carcinoma (OSCC) is characterized by high mortality rate and rising incidence in developed countries. Epigenetic modifications are changes in gene expression that are not coded in the DNA sequence. DNA methylation of CpG islands in promoters causes transcriptional silencing of genes and malignant transformation. The genes selected for our investigation are involved in cellular processes that are crucial for malignant transformation including cell cycle control (p16), apoptosis (DAPK), Wnt signaling (APC), cell-cell adhesion (E-cadherin), and DNA repair (MGMT, WRN). In 77 patients with OSCC, methylation was determined by methylation-specific PCR. Hypermethylation of p16 gene promoter was detected in 58.4% of samples, MGMT in 33.76%, DAPK in 36.36%, WRN in 23.37%, E-cadherin in 42.86%, and APC in 18.18% of OSCC samples. Patients with E-cadherin promoter methylation had significantly worse overall and cancer-specific survival (χ², p<0.05), while hypermethylation of other genes did not have prognostic significance. E-cadherin promoter hypermethylation could be molecular marker for the poor survival in OSCC.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.80

PATTERN OF TRISOMY 1Q IN HEMATOLOGICAL MALIGNANCIES: A SINGLE INSTITUTION EXPERIENCE

Đorđević Vesna^a, Denčić-Fekete Marija ^a, Jovanović Jelica^a, Drakulić Danijela^b, Stevanović Milena^b, Janković Gradimir^a, Gotić Mirjana^a

^aInstitute of Hematology, Clinical Center of Serbia, Belgrade, Serbia

^bInstitute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

An extra copy of 1q usually originates from the translocated unbalanced derivative chromosome, isochromosome, or ‘‘jumping translocation.’’ We report a pattern of partial trisomies and unbalanced whole-arm translocations of 1q in 10 patients: 5 with myelodysplastic syndrome, 3 with acute myeloid leukemia, and a single patient with acute lymphoblastic leukemia and myeloproliferative syndrome. The trisomy of 1q was registered as the sole karyotype aberration in one patient, while it was accompanied by a limited number of additional chromosomal changes in nine patients. Analyzed patients are a subset of a larger group of 92 adults carrying a wide variety of chromosome 1 anomalies within a complex cytogenetic context observed over a period between 1994 and 2006 in a panel of 3786 hematologic patients at the Institute of Hematology in Belgrade. Chromosomes were prepared according to standard procedures and HG-banding. Conventional cytogenetics was supplemented by fluorescence in situ hybridization (FISH) with a probe specific for the paracentric region of 1q. Whole-arm 1q translocations involved chromosomes: Y, 7, 14, 15, 16, 16 and 19. This study suggests that extra copy of 1q as the sole cytogenetic abnormality may be sufficiently mutagenic to favor leukemogenesis and hematopoietic tissue degeneration (trilineage myelodysplasia).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.81

GENETICS IN PHARMACOLOGY: AN INDIVIDUALIZED MEDICINE

Jelenković Ankica

Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Prescribing drugs is a serious responsibility. The effects of medication varies from person to person, which can be, beside other reasons, due to the fact that human genetic profiles are not identical. The first such observations appeared more than 50 years ago. That was the muscle relaxant suxamethonium chloride that prolonged apnea in some people, as well as the drugs metabolized by N-acetyltransferase with very different blood concentrations of isoniazid and procainamid. It is known that genetic factors influence drugs' pharnmacokinetic and pharmacodinamic. The term pharmacogenetcs is used since late 1950s. It is generally regarded as the study or clinical testing of genetic variation that gives rise to differing response to drugs. Phenomenon of individual variations to drugs progressed toward pharmacogenetic science which promise to incorporate into clinical practice a concept of „tailor-made medicine“, individualzed medicine, in which drugs and drug combinations become coordinated with each individual's unique genetic profile. The wider use of pharmacogenetic testing is viewed by many as an outstanding opportunity to optimize prescribing safety, efficacy and cost.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.84

FAMILY HISTORY OF GRAVES-BASEDOW DISEASE AND ENVIRONMENTAL RISK FACTORS IN ETIOLOGY OF THIS DISEASE

Živanović Dušica¹, Šipetić Sandra²,Stamenković-Radak Marina³ and Milašin Jelena⁴

¹Dept of Gynecology and Obstetics, Health Center Ćuprija, Ćuprija;²Institute of Epidemiology, Medical School, University of Belgrade;³Faculty of Biology, University of Belgrade;⁴Institute of Biology and Genetics, School of Dentisty, University of Belgrade

Graves-Basedow disease (GBD) is a common multifactorial syndrome, determined by several genes and environmental factors. Our study aimed at unveiling risk factors for developing GBD and to compare risk factors in affected individuals with positive family history (PFH) and individuals without PFH of GBD. This cross-sectional study was conducted in Ćuprija and icluded 262 individuals (132 with GBD and 130 healthy subjects). Data were collected on basic demographic characteristics, exposure to various chemical and physical agents, stress, smoking and PFH of GBD. Affected individuals were older (above 50) (p=0,020), exposed to stress (p=0,024) to physical agents (p=0,031), and had significanly (p=0,000) more relatives with GBD than the control. Among the affected individuals with PFH of GBD, the number of women was significantly higher (p=0,000), than in the affected individuals without PFH. Gender, age, PFH and exposure to physical agents were identified as potential risk factors for the increased incidence of GBD. Different risk factors are probably responsible for Graves-Basedow disease among the affected individuals with and without PFH.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.85

POPULATION GENETICS IN PRECLINICAL PRACTICE: PRESENTATION OF 18 YEARS WORK CARRIED AUT AT GENETIC COUNSELLING OFFIS OF THE MEDICAL PRACTICE IN JAGODINA

Janakova Zorica

Healthcare Center, Jagodina

The work shows principles successfully organized prevention of chronic comman noncontagious diseases and basic levels of activity carried out at this family counselling offis. From the aspect of populational genetics and preclinical practice and using the examples of Diabetes mellitus, we establish the genetical, constitutional, physiological and environmental parameters of the most effective selection of the persons (families) in order to diagnose liability .The work shows Population and Genetic Questionnaire of pregnant and child-bearing woman in the systematic procedure of screening, long-term monitoring and control genetic, physiological and environment health of the population.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.86

CASE STUDY OF POSSIBLE FAMILIAL ANTIPHOSPHOLIPID SYNDROME

Popović-Kuzmanović Dragana¹, Novaković Ivana², Stojanović Ljudmila¹, Miković Danijela³, Mirković Duško⁴, Luković Ljiljana²and Aksentijević Ivona⁵

¹Clinical center ”Bežanijska Kosa”,²³Institute of Biology and Human genetics, School of Medicine, University of Belgrade,³Transfusion Institute of Serbia, ⁴Institute of Biochemistry, KCS, Belgrade, Serbia,⁵National Institutes of Health, Arthritis and Rheumatism Branch, Bethesda, USA

Antiphospholipid syndrome (APS) may have a familial association. This familial tendency could be genetically determined. In this study we analyzed family consisted of 7 members, with three affected members: father and two suns. The diagnosis was established by clinical signs, imaging methods, presence of anti cardiolipin and anti β₂ glicoprotein I antibodies, homocysteine levels, coagulation factors as well as protein C, protein S, antithrombin III deficiencies and APC resistance. DNA polymorphisms in genes encoding for factor V (G1691A), prothrombin (G20210A), MTHFR (C677T and A1298C) were analyzed by PCR/RFLPS method. Plasma Hcy was measured by HPLC method. Father and two suns had pulmonary embolism. Nobody have positive ANA, dsDNA and RF. The values of anti cardiolipin anti β₂ glycoprotein I antibodies were border line positive. Hcy levels and platelet number were within normal range. All members of family had low value of factor V, and father and both of suns also had low factor II, VII and X. Father and his suns had low protein C and S, but they are on anticoagulant therapy. The results of genetic studies will be discussed in the context of biochemical and clinical phenotypes. Further investigations will include analyses of new DNA polymorphisms, which could be associated with family disease.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.87

PHARMACOGENETICS, DRUG INTERACTIONS AND ADVERSE DRUG REACTIONS

Jelenković Ankica

Institute for Biological Research "Sinisa Stanković“, Belgrade, Serbia

Over half a century ago it has been recognized that genetic determined variations between people contribute to interindividual differences in the response to the same dose of the same drug, given separately or together with other drugs. Pharmacogenetics is a growing field of research and medicine focuses on the interaction between a single gene variation and drug therapy. Genetic polymorphism are involved in drug efficacy, drug-drug interactions and adverse drug reactions (ADR) variability, because most of drug effects are defined by the interplay of multiple gene products through the entire drug pathway in the human body, such as metabolizing enzymes, transporters, receptors and/or ion channels. Millions of people worldwide are affected per annum by ADR, which are among leading causes of death. Pharmacotherapy improvement, especially that of medications with narrow therapeutic indices, is expected to be obtain with identification of gene variants encoding key components of drug targets (i.e. receptors) and all aspects of drug disposition (i.e. absorption, distribution, metabolism and excretion) responsible for increased risk of adverse drug reactions and unwanted drug interaction.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.88

CANDIDATE GENE POLYMORPHISMS AND SUSCEPTIBILITY TO ABDOMINAL AORTIC ANEURYSM

Cvetković Dragana¹, Cvetković Slobodan², and Novaković Ivana³

¹Faculty ofBiology, University of Belgrade, Belgrade

²Clinic for Vascular Surgery, Institute of Cardiovascular diseases Clinical Center of Serbia

³Institute of Human Genetics, School of Medicine, University of Belgrade

Abdominal aortic aneurysm (AAA) is a common vascular disease with high fatality rate. Genetic polymorphisms potentially related to susceptibility to aneurysm formation and disease outcome have attracted much interest. The aim of this study was to examine the association between AAA and following candidate gene polymorphisms: -1562 (C→T) of matrix metalloproteinase (MMP) 9 gene, common e2/e3/e4 polymorphism of apolipoprotein E (APOE) gene, I/D of angiotensin converting enzyme gene (ACE), 677 (C→T) of methylenetetrahydrofolate reductase (MTHFR) gene and -819 (C→T) of interleukin (IL) 10 gene. The study included patients who underwent surgery for AAA during 2005-2006 at the Institute for Cardiovascular Diseases CCS and healthy controles comparable for sex and age. Significant association with AAA was found only for ACE I/D polymorphism (OR=2.897, 95% CI 1.409-5.955, p=0.003). None of the other polymorphisms was significantly related with susceptibility to AAA. Though it is not likely that a single gene polymorphism has a key role in aneurysm formation, candidate gene approach provides better understanding of pathogenesis and individual susceptibility to AAA and may provide potential therapeutic targets.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.89

THE ASSOCIATION OF IL-6 -174G/C GENE POLYMORPHISM WITH CAROTID ATHEROSCLEROSIS IN POPULATION OF SERBIA

Stojković Ljiljana¹, Živković Maja¹, Radak Đorđe², Kolaković Ana¹, Stančić Olja¹, Stanković Aleksandra¹ and Alavantić Dragan¹

¹Institute of Nuclear Sciences VINČA, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Serbia

²Dedinje Cardiovascular Institute, Vascular Surgery Clinic, Belgrade, Serbia

If chronic inflammation plays a causal role in atherogenesis, individuals with proinflammatory gene variants would be expected to develop atherosclerosis. The IL-6 gene were chosen because is an important component of the upstream inflammatory process and the -174G/C polymorphisms already implicated in cardiovascular disease. The aim of was to associate the IL-6 -174G/C polymorphism with development of carotid plaques. Materials and methods: Genomic DNA was isolated from blood cells of 278 controls and 136 patients with carotid atherosclerosis. The genotypes were determined by tetra-primer ARMS PCR and visualized by agarose gel electrophoresis. Results: The genotype distribution wasn’t significantly different in patients (GG 39.71%, GC 44.12%, CC 16.18%) compared to controls (GG 41.73%, GC 46.40%, CC 11.87%). Conclusion: The IL-6 gene polymorphism is not associated with advanced atherosclerotic phenotype such as plaque presence, in population of Serbia. As IL-6 is expressed in human atherosclerotic plaques there is the possibility that the level of its expression plays the more important role in this clinical phenotype than genotype of investigated polymorphism.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.90

MATRIX METALOPROTEINASE-9 GENE POLYMORPHISM -1562C/T AS SUSCEPTIBILITY FACTOR FOR CAROTID ATHEROSCLEROTIC PLAQUE DEVELOPMENT

Kolaković Ana¹, Stojković Ljiljana¹, Radak Đorđe², Đurić Tamara¹, Radak Sandra², Stanković Aleksandra¹, Alavantić Dragan¹and Živković Maja¹

¹Institute of Nuclear Sciences VINČA, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Serbia;²Dedinje Cardiovascular Institute, Vascular Surgery Clinic, Belgrade, Serbia

The matrix metalloproteinase 9 (MMP-9) has been shown to play role in VSMC migration and proliferation by degradation of the basement membrane. MMP-9 expression is increased after vascular injury. The MMP-9 -1562 C/T gene polymorphism results in higher promoter activity of the T allele. The higher prevalence of –1562T allele was associated with coronary artery stenosis. The aim was to investigate the association of the MMP-9 -1562C/T polymorphism and susceptibility for development of carotid plaques. Genomic DNA was isolated from blood cells of 278 control subjects and 136 patients with carotid atherosclerosis. The genotypes were determined by tetra-primer ARMS PCR method previously developed by us and visualized by MADGE. We did not find the significant difference in genotype frequencies between controls: CC 70.82%, CT 27.05%, TT 2.14%, and patients: CC 74.26%, CT 25.74%, TT 0%. The allele frequencies didn’t differ as well (controls: C 0.84, T 0.16, patients: 0.87, 0.13. MMP-9 gene polymorphism -1562C/T is not the susceptibility factor for development of carotid atherosclerotic plaques in population of Serbia.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.91

DISTRIBUTION OF PARAOXONASE 1 CODING REGION POLYMORPHISMS IN SERBIAN POPULATION

Pejin Ivana¹, Buzadžić Ivana¹, Janković-Oreščanin Biljana²and Šuput-Tomaš Ljiljana²

¹ Department of Human genetics and prenatal diagnosis, Clinical Center Zvezdara, Belgrade, Serbia

² Trasfusiology service, Clinical center Zvezdara, Belgrade, Serbia

Human serum paraoxonase 1 (PON1) is associated with high-density lipoproteins and is coded by a gene located on the 7q21.3-22.1 region of chromosome 7. PON1 hydrolyzes the active metabolites of several organophosphorus insecticides and neural agents and metabolizes oxidized lipids present the most in oxidized low density lipoproteins. L55M and Q192R are the most studied pon1 coding region polymorphisms, and intrapopulation and interethical variability in PON1 levels and activity is determined by the Q192R and L55M. PON1 has been widely investigated, especially for its involvement in atherosclerosis and age-related diseases.The aim of this study was to determine the frequencies of pon1 polymorphisms Q192R and L55M in Serbian population. The following genotype frequencies were determined: 60 % QQ, 34 % QR, 6,5 % RR for Q192R; 47 % LL, 42 % LM, 11 % MM for L55M. The most frequent haplotype combination was QQ / LL (26%).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.92

THE PREVALENCE OF FII A19911G I FII C20221T GENE VARIANTS IN THROMBOPHILIC SERBIAN POPULATION

Đorđević Valentina¹, Nestorović Aleksandra¹, Tomić Branko¹, Miljić Predrag², Kovač Mirjana³, Miković Danijela³, Antonijević Nebojša⁴, Rakićević Ljiljana¹ and Radojković Dragica¹

¹Institute of molecular genetics and genetics engineering, Belgrade;²Institute of Hematology, Clinical Centar of Serbia, Belgrade;³Institute of Blood Transfusion, Belgrade

⁴Institute for Cardiovascular diseases, Clinical Centar of Serbia, Belgrade

Thrombophilia is multifactorial disorder, which is linked with wide range of clinical manifestations including deep venous thrombosis (DVT) and recurrent pregnancy loss (RPL). A polymorphism in the last intron of the FII gene A19911G is associated with slightly elevated plasma prothrombin level but its role as a thrombophilic risk factor for is not clearly defined. Recently, a new 3'UTR prothrombin variant C20221T was reported. The aim of our study was to establish the prevalence of these prothrombin gene variants in a group of 100 patients with DVT, 100 healthy controls, 50 women with RPL and 20 controls with no previous history of RPL. Polymorphisms were detected by polymerase chain reaction, followed by digestion with specific restriction enzymes. The FII 19911GG genotype were overrepresented in patients vs control groups (23% vs 19% patients with VTE and 26% vs 10% for patients with RPL) but difference in frequencies was not statistically significant. FII C20221T variant was found neither in the control subjects, nor in the patients groups. Our study suggests that FII A19911G and FII C20221T variants are not an important thrombophilic risk factor in our population.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.93

DETERMINATION OF ACE GENE POLYMORPHISM DISTRIBUTION IN DIABETES NEPHROPATHY DEVELOPMENT

Ilić Vesna¹, Ilić Miroljub² and Magić Zvonko¹

¹Institute of Medical Research, Military Medical Academy, Belgrade

²Institute of Diabetis and Metabolic Diseases, Clinicla center Serbia, Belgrade

Clinicalhallmarks of diabetic nephropathy (DN),a major microvascular complication, include a progressive increasein urinary albumin excretion in association with an increase in bloodpressure and to end-stage renal failure.Renin-angiotensine system (RAS) hyperactivity and the presence of corresponding genotypes of the RAS components, such as angiotensin-converting enzyme (ACE),predispose the increasing risk of renal failure induced with hypertension. To analyze a distribution of the ACE gene polymorphism in patients with type 1 diabetes arranged in three groups according to increasing level of DN. ACE gene-insertion/deletion- polymorphism,was genotyped in a group of 80 patients,out of which 31 were normoalbuminuric ,19 microalbuminuric and 22 proteinuric patients. The frequency of ID and DD genotype in a group of microalbuminuric patients was 63,15 %, in proteinuric pts. 77,27 % compared to normoalbuminuric 70 %.Percentage of "worse" polymorphisms (ID+DD) wasn’t increased in a group of patients with terminal DN. The observed ID and DD allelic distribution did not show the increased frequencies of these allele form related with DN progression.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.94

APOE GENE POLYMORPHISM IN PATIENTS WITH CORONARY HEART DISEASE

Đan Igor¹, Đan Mihajla², Obreht Dragana², Jovanović Nataša³, Stokić Edita⁴,

Sakač Dejan⁵and Vapa Ljiljana²

¹Institute for health care of Children and Youth of Vojvodina, ²Faculty of Sciences, Dept of Biology and Ecology, ³Faculty of Medicine, ⁴Clinical Center Vojvodina, Dept of Endocrinology, Diabetes and Metabolism, ⁵Institute for Cardiovascular Diseases Sremska Kamenica, Clinics for cardiology; Novi Sad

Cardiovascular diseases are leading massive, chronical and uninfectious diseases and unfortunately represent leading mortality factor in modern men. The aim of this paper is to determine apoE allelic variants in a group of patients with coronary heart disease (CAD), and to compare their frequencies with health control group. RFLP-PCR analysis of exon 4 in a group of healthy control revealed the frequency of e3 allele 86%, e4 12% and e2 2%. In a group of patients with CAD increased frequency of e4 allele 23.33%, and decreased frequency of e3 allele 75%, while the frequency of e2 allele was 1.67%. Distribution analysis of apoE alleles revealed statistically significant higher frequency of e4 allele in a group of patients, as expected and previously determined as risk factor for CAD development. Beside this fact, statistically significant higher number of persons with e3e4 genotype was found in a group of patients comparing to control group. Comparation of analysed antropometric and biochemical parameters showed statistically significant higher values of blood pressure, BMI and fat percent, triglycerides, insulin (HOMA IR) and CRP in a group of patients.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.95

ASSOCIATION OF PARKINSON’S DISEASE AND POLYMORPHISM

IN ENVIRONMENT RESPONSIVE GENES

Buzadžić Ivana¹, Pejin Ivana¹, Ilić V. Tihomir², Janković-Oreščanin Biljana³

and Šuput-Tomaš Ljiljana³

¹Department of Human Genetics and Prenatal Diagnosis, Clinical Center „Zvezdara“, Belgrade, Serbia; ²Clinic for Neurology, Military Medical Academy, Belgrade

³Trasfusiology service, Clinical center Zvezdara, Belgrade, Serbia

Parkinson’s disease (PD) is a progressive neurodegenerative disorder, clinically characterized by bradykinesia, rigidity, resting tremor and postural instability. It has been suggested that polymorphism in enzymes involved in oxidative metabolism and detoxification could be involved in predisposition to PD. Studies have hypothesized that individuals with poor metabolizing status, based on cytochrome P4502D6(CYP2D6*3 and*4) genotype could be at risk to PD (EM,p=0,06, IMp=0,40, PMp=0,07). Polymorphism in enzymes, wich generate free radicals or those involved in dopamine neurotransmission such as cytochrome P4502E1*5B (CYP2E1*5B), glutathione S tranferases (GSTs), which are known to interact with environmental exposures, could be implicated in the pathogenesis of PD. A significant association was observed between the GSTP1*B allele zygosity with PD(GSTP1*A/*B-49,5%/30,37%; *B/*B-5,31%/1,05%).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.96

ANALYSIS OF THE -1562 C>T POLYMORPHISM IN MMP-9 PROMOTER IN PATIENTS WITH HEAD AND NECK CARCINOMA

Bošković Marija, Popović Branka and Milašin Jelena

Institute of Biology and Genetics, School of Dentisty, University of Belgrade

MMP-9 or Gelatinase B, a member of the matrix metalloproteinase family (MMPs), play an important role in theinvasive behavior of a number of cancers including head and neck squamous cell carcinoma (HNSCC) and basal cell carcinoma (HNBCC), and increased expression of MMP-9 is correlatedwith invasive and metastatic HNSCC and HNBCC. The prognostic role of MMP-9 is still unclear. Theaim of this study was to investigate the role of the -1562 C-to-T polymorphism in MMP-9 promoter (abbreviated MMP-9 -1562 C>T polymorphism). Genomic DNAs were obtained from peripheral blood cells of patients with SCC and BCC and healthy controls. The MMP-9 (–1562 C>T) polymorphisms were determined by the polymerase chain reaction-based restriction fragment length polymorphism (PCR–RFLP). There was no significant difference in the genotype distribution between patients and controls. Our data suggest that the -1562 C>T polymorphism doesn’t have a significant role in head and neck squamous cell carcinoma and basal cell carcinoma. Therefore, this polymorphism couldn’t be used as a risk and prognostic factor.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.97

DETERMINATION OF NUMBER OF CAG REPEATS WITHIN HTT GENE BY CAPILLARY ELECTROPHORESIS

Dobričić Valerija, Novaković Ivana and Kostić Vladimir

Institute of Neurology, Clinical Centar of Serbia

Huntington disease (HD) is an autosomal dominant neurological disorder with prevalence between 3 and 7 per 100 000 in European population. HD causing mutation is CAG trinucleotide repeat expansion within exon 1 of the HTT (IT15) gene.The number of CAG repeats in normal alleles is less than 26. Alleles with 27-35 CAG repeats are not associated with an abnormal phenotype but they have significant propensity to expand during parental transmission. Disease-causing alleles contain more than 36 CAG repeats, while alleles that contain 36-39 repeats are characterized by reduced penetrance, and those with 40 and more repeats are characterized by full penetrance.Routine genetic testing is based on PCR amplification of CAG repeats containing region and subsequent analysis by polyacrylamide gel electrophoresis. In our laboratory we established contemporary and more sensitive method for exact repeat size determination based on separation of fluorescently labeled PCR products by capillary electrophoresis using ABI310 genetic analyzer (Applied Biosystems) and GeneMapper 4.0 software.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.98

TRANSLOCATION (5;6;12) ASSOCIATED WITH RESISTANCE TO IMATINIB MESYLATE IN CHRONIC MYELOID LEUKEMIA

Denčić-Fekete Marija¹, Đorđević Vesna¹, Tiziana Storlazzi Clelia³, Janković Gradimir¹, Bogdanović Andrija¹, Jovanović Jelica¹, Rocchi Mariano², Todorić-Živanović Biljana², Strnad Milica² and Gotić Mirjana¹

¹Institute of Hematology, Clinical Center Serbia, Belgrade, Serbia

²Military Medical Academy, Belgrade, Serbia

³Department of Genetics and Microbiology, University of Bari, Bari, Italy

We present a patient with the t(9;22)-positive chronic myelogenous leukemia (CML) who became resistant to imatinib mesylate (Glivec) therapy and that was associated with the appearance of t(5;6;12) in the same cell with t(9;22):[46,XX,t(5;6;12)(q14?;q21?;q23?),t(9;22)(q34;q11)]. Philadelphia chromosome was detected by standard cytogenetic technique and RT-PCR analysis. Additional abnormalities were precisely defined by FISH and M-FISH methods. The patient did not show any clinical or laboratory signs of disease progression. She remains well and in stable chronic phase more than two years since the evidence of an additional complex cytogenetic change has been observed. The resistance to imatinib therapy seems to have coincided with the appearance of t(5,6,12). This is the first reported case of karyotype evolution in this disease which is temporally associated with the induction of resistance to imatinib but without influencing the clinical course of leukemia towards a more anaplastic and aggressive form. The absence of clinical progression of disease suggests that this complex translocation, despite its theoretically possible role in induction or facilitation of resistance to imatinib, is unrelated to pathways of karyotype evolution to advanced clinical stages of CML.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.99

PRENATAL AND POSTNATAL MOLECULAR STUDY OF SMA USING RESTRICTION ENZYME ASSAY

Mišković Marijana¹, Lalić Tanja¹, Radivojević Danijela¹, Guć-Šćekić Marija¹, Zamurović Dragan² and Vlahović Gordana²

¹Labaratory for medical genetics, Mother and Child Helthcare Institute of Serbia „Dr Vukan Čupić”, Belgrade

²Department of Neurology, Mother and Child Helthcare Institute of Serbia „Dr Vukan Čupić”, Belgrade

Spinal muscular atrophy (SMA) is the second most frequent autosomal recessive disease. According to the age of onset and severity of the clinical manifestations, SMA is classified into three types. SMN gene exists in two nearly identical copies-SMNt and SMNc, but only deletion/mutation in SMNt is seen to cause SMA (98%). Deletion of NAIP gene is observed more often in severe SMA (45%). In this report we present the molecular analysis of the SMN and NAIP gene in 41 SMA patients and 27 prenatal samples, from pregnant women with affected child. DNA extracted from blood samples, CVS and amniotic fluid was amplified by PCR. Exons 7 and 8 of SMN gene and exons 5 and 13 of NAIP gene were amplified. Restriction enzymes digestion (restriction enzyme assay) with DraI and DdeI was used to separate two SMN copies. Results revealed the homozygous deletions of exons 7 and 8 of the SMN gene in 76% (31/41) and deletion of exon 5 of the NAIP gene in 20% (8/41). Exon 7 deletion was found in one case. In 6 prenatal cases were found deletion of exons 7 and 8; in one case was present only deletion of exon 7. These findings have important implications for genetic counseling and pre- and postnatal diagnosis of SMA.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.100

SURVEY OF A CASE OF GENDER REVERSION

Durković Jasmina, Cemerlic Dejan, Anđelić Luka and Drakulić Danijela

¹Department of Genetics, Hospital Subotica, ²Department of Obstetrics and Gynecology, Hospital Subotica, ³Institute of molecular genetics and genetics engineering, Belgrade, Serbia

In persons with gender reversion there is no harmony between the gonosomal constitution and the phenotype. A case of a woman with the karyotype 46, XY accompanied by a gonadal dysgenesis, i.e. by a disturbance of testis differentiation in a genetic man is presented.By a cytogenetic analysis, a karyotype 46, XY was obtained on 30 metaphases of all the cells with the deletion of a short branch Y. The Fluorescence in situ hybridization (FISH) from the peripheral blood lymphocite was performed using DXZ1/DYZ3 tests for the centromeric region of the chromosomes X and Y. In 96% of the cells 46, XY karyotype was identified and in the remaining 4% of the cells 45, X0 karyotype was found. With a primer typical of SRY gene on the chromosome Y, the polymerase chain reaction (PCR) was performed and the absence of SRY gene was proven. A detailed laparoscopy of uterus and gonads was done along with the gonadal tissue biopsy. The uterus was extremely hypoplastic, whereas in the place of both ovaries fibrosal white-like straps with no traces of ovarial function were detected.It is extremely important to PCR detect a SRY gene on the chromosome Y at women with a male karyotype, as all SRY positive women are exposed to a high risk of malignant gonadoblastoma during adolescence.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.101

COMPARATIVE CYTOGENETIC ANALYSIS OF CHROMOSOMAL ABERRATIONS AND PREMATURE CENTROMERE DIVISION IN LYMPHOCYTES OF PERIPHERAL BLOOD IN PERSONS PROFESSIONALLY EXPOSED TO RADIONUCLIDES

Jovičić Dubravka¹, Rakić Boban², Vukov Tanja³, Milačić Snežana², Pajić Jelena², Stevanović Milena⁴ and Drakulić Danijela⁴

¹The Faculty of Applied Ecology Futura, Singidunum University, Belgrade

²KCS – Institute for medical and radiology protection „Dr Dragomir Karajović“, Belgrade

³Institute for biological research "Sinisa Stanković“, Belgrade;⁴Institute of molecular genetics and genetics engineering, Belgrade, Serbia

The aim of this study was the comparative analysis of chromosomal aberrations (CA) and premature centromere division (PCD) in persons professionally exposed to radionuclides. Biological dosimetry was performed by conventional cytogenetic technique. The presence of PCD was confirmed by fluorescent in situ hybridization (FISH). Examinations were performed on 50 persons professionally exposed to radionuclides (average age 45.24±1.18, average exposition time 17.96±1.15 years) and 30 control persons (average age 36.07± 1.11, average years of employment 10.77±1.07 years). The results show that frequencies of CA and PCD in lymphocytes of peripheral blood are significantly higher in patients exposed to radionuclides than in control group (Mann Whitney U Test, p<0.01). Moreover, in group of exposed patients the number of isochromatid breaks was significantly higher in smokers in respect to non-smokers. There is no correlation between frequencies of chromosomal aberrations, except for acentric fragments, and tPCD (PCD present in more then 10 chromosomes per metaphase). Our results indicate that the presence of PCD can be used as one of cytogenetical marker in persons professionally exposed to radionuclides.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.102

COPY NUMBER CHANGES IN PATIENTS WITH DISORDERS OF SEX DEVELOPMENT AND FUNCTIONAL ANALYSES OF CANDIDATE GENES

Daggag Hinda, White Stefan and Sinclair Andrew

Murdoch Children’s Research Institute and Dept. of Paediatrics, University of Melbourne, Royal Children’s Hospital, Melbourne, Australia.

Disorders of sex development (DSD), ranging in severity from genital abnormalities to complete sex reversal, are surprisingly common and as such represent a major pediatric concern. The cause of these disorders is most often a disruption of the genetic programs that regulate development of testes or ovaries. Although a number of genes have been identified in these developmental pathways, in many cases of DSD the causative mutations cannot be identified. We have used the Affymetrix 6.0 whole genome SNP array to perform whole genome copy number analysis on genomic DNA from 33 individuals with gonadal dysgenesis. Rearrangements affecting known sex determination genes were a duplication of the X chromosome including DAX1 in a 46,XY female, as well as a 1.2 Mb deletion upstream of the SOX9 locus that caused sex-reversal without campomelic dysplasia in a 46,XY female. Several other potentially causative rearrangements were identified, including a duplication of the SOX3 gene in a 46,XX male and a 50 kb deletion immediately downstream of GATA4 in a 46,XY female. Both of these genes have been suggested to play a role in gonadal development in animal models without previous supporting evidence in humans.These findings will stimulate molecular analysis of a number of genes in gonadal determination and differentiation, and support the hypothesis that mutations affecting the regulation of known genes are responsible for a significant number of DSD cases.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.103

TELOMERE DYSFUNCTION INDUCED FOCI CORRELATES POSITIVELY WITH INCIDENCE OF SISTER CHROMATID EXCHANGES IN CELLS OF FANCONI ANEMIA PATIENTS

Joksić Ivana¹, Guć-Šćekić Marija², Vujić Dragana², Petrović Sandra³, Leskovac Andreja³

¹Clinic of Gynecology and Obstetics „Narodni front“

² Mother and Child Helthcare Institute of Serbia „Dr Vukan Čupić”, Belgrade

³ Institute of Nuclear Sciences VINČA

Telomeres consist of repeated DNA sequences and proteins that lie at the chromosome termini and are critical to stability of internal chromosome sequences. Telomeres restrict chromosome end resection by exonucleases and prevent the improper activation of checkpoint response factors and DNA damage response pathways. The development of the TIF (telomere dysfunction induced foci) assay that is based on colocalization of the DNA repair factors 53BP1 and γH2AX with telomeres, enables measurement of telomere uncapping in different tissues. In 9 primary fibroblast cell lines, obtained from patients presenting with bone marrow failure syndrome and Fanconi anemia phenotype, baseline incidence of SCE, T-SCE, γH2AX and TIF were examined. The average incidence of SCE was 4.42±0.96 (3.38 to 6.5) per cell, whereas incidence of T-SCE was 1.91±0.81 (1 to 3.33). Baseline level of γH2AX foci was 2.27±1.55 (0.8 to 4.2), whereas average incidence of TIF was 2.97±1.47 (1.44 to 5) per cell. Statistically significant positive correlation between incidence of SCE and TIF was observed (0.77 p<0.05) indicating that uncapped telomeres in these cells might have arisen via process of homologous recombination.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.104

GLI3 GENE MOLECULAR ANALYSIS OF PATIENTS WITH PALLISTER-HALL SYNDROME

Radivojevic Miloš¹, Keckarović-Marković Milica¹, Dacković Jelena², Apostolski Slobodan², Brajušković Goran¹ and Romac Stanka¹

¹University of Belgrade, Faculty of Biology, Serbia

²University of Belgrade, School of Medicine, Institute of Neurology, Serbia

Pallister-Hall syndrome (PHS) is a rare autosomal dominant multisystem disorder caused by mutations in a Glioma-Associated Oncogene Homolog 3 zinc-finger transcription factor (GLI3) gene. Here we present a molecular analysis of two patients, a mother and a daughter, with a typical PHS phenotype. Genomic DNA was isolated from blood samples (QIAamp blood purification kit, Qiagen Inc., Valencia, CA, USA). The GLI3 coding exons were amplified using PCR, and sequenced (BigDye Terminator kit ver. 1.1 Applied Biosystems, CA, USA) by capillary electrophoresis (3130 Genetic Analyzer, Applied Biosystems, CA, USA). Sequencing analysis revealed a heterozygous truncating nonsense mutation (c.2146C®T), located in the 2^nd third of the gene. The type and localization of the mutation are in consistency with a proposed model by which GLI3 caused constitutive repression is a molecular basis of PHS. GLI3 acts as both a transcriptional activator and a repressor in the sonic hedgehog pathway. Frameshift/nonsense mutations in the 2^nd third of GLI3 (c. 1998-3481), affecting regions downstream of the DNA-binding domain, give truncated protein that acts as a constitutive repressor.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.105

RAPID PRENATAL DIAGNOSIS OF ANEUPLOIDY USING QUANTITATIVE FLUORESCENCE-PCR (QF-PCR) IN SERBIA

Lukić Jelena, Anđelković Marina and Bugarić Predrag

Helixlab, Belgrade

The most commonly encountered chromosomal disorders in newborns are trisomies of chromosomes 13, 18, 21 and sex chromosomes (Patau, Edwards, Down, Klinefelter and Turner’s syndrome). Trisomies of other chromosomes usually lead to spontaneous miscarriage. In prenatal diagnosis of fetal aneuploidies QF-PCR method gives new possibilities for rapid and precise diagnosis of these anomalies. Main advantage of this method is extremely small amount of specimen acquired (CVS, amniotic fluid, fetal blood or fetal tissue), effective distinction between mother cells and cells deriving from fetus, detecting mosaicisms and getting results in 24 hours period time. The method excludes possibility of human factor mistake since this process is almost entirely automated. Overall, 889 samples have been analyzed in this laboratory so far. Following results were found: 7 trisomies of chromosome 21; 6 trisomies of chromosome 18; 3 trisomies of chromosome 13 and 1 mosaicism of chromosome 21.This method proves to be quick, simple and efficient way of detecting selected chromosomal anomalies, which is of major importance in prenatal diagnosis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.106

THE ROLE OF p53 GENE AND HUMAN PAPILLOMA VIRUS IN PATHOGENESIS OF ORAL SQUAMOUS CELL CARCINOMAS (OSCCS)

Popović Branka¹, Jekić Biljana², Novaković Ivana²and Milašin Jelena¹

¹Institute of Biology and Genetics, School of Dentisty, University of Belgrade

²Institute of Human Genetics, School of Medicine, University of Belgrade

OSCCs are associated with poor prognosis, and despite advances in therapy no improvement has been achieved in survival. Efforts are directed toward finding of crucial markers that could more accurately predict tumor behavior. One of the most important genes contributing to OSCCs pathogenesis is p53. Its protein product, p53, is involved in cell cycle control and apoptosis. Mutations affecting p53 gene could lead to deregulated cell progression. Also, the function of p53 proteins might be disabled by binding to proteins of the human papilloma virus (HPV). In this study was done an attempt to establish the role of p53 gene and HPV in pathogenesis of OSCCs. A series of 60 paraffin embedded OSSC samples were analyzed for the presence of mutations in exons 5-8 of p53 gene by SSCP, followed by sequencing. PCR detection of HPV 16 was done by amplification of the region of E6 genes. 60% of samples were positive for p53 mutation, while HPV was detected in 10% of the cases. Finding of p53 mutations with incresed rate in higher tumor stages and grades, might suggest that p53 gene has imortant role in patogenesis of OSCCs, but without significant HPV involvment.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.107

ANALYSIS OF c-myc AND c-erbB-2 ONCOGENES IN HISTOPATHOLOGICALLY TUMOUR-FREE SURGICAL MARGINS IN PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA AND BASAL CELL CARCINOMA

Manasijević Maja, Nešić Branka, Jelovac Drago, Ilić Branislav, Popović Branka

and Milašin Jelena

Institute of Biology and Genetics, School of Dentisty, University of Belgrade

Cancers of head and neck consistently ranks as one of the top ten cancers worldwide. C-erb and c-myc genes mutations have been considered as selective and sensitive DNA markers of cancer cells. Their identification in histopathologically tumour free margins could be of great importance for the recurrence risk evaluation. Aim of this study was to assess the status of c-myc and c-erb in histologically free tumor margins in patients with OSCC and BCC. Specimens were obtained from patients diagnosed with OSCC and BCC, who underwent surgery. DNA extraction for molecular analysis was done on tumours margins, previously confirmed by a pathologist as histologically free. Differential PCR was used for the detection of c-myc and c-erbB-2 amplification. Amplification of c-erbB-2 gene was found in 5 out of 50 patients with OSCC, whilst 7 harboured c-myc amplification. C-myc deletion was detected in 3 cases. C-erb overexpression was observed in 7 of the 50 BCC tissue slides, whereas increased c-myc oncogene expression concerned 8 of 50 cases. Molecular analysis of free surgical margins, targeting oncogenes, could enable the selection of OSCC and BCC patients at higher risk for tumour recurrence.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.108

DETECTION OF PRE-MANIFESTED CHANGES OF CERVIX UTERI WITH COMET ASSAY

Fatović-Milićević Božica¹, Mitić-Ćulafić Dragana ², Vuković-Gačić Branka², Berisavac Milica³, Lakić Nada³ and Knežević-Vukčević Jelena²

¹Polyclinic Vizim, ²Department for mycrobiology, Faculty of Biology, University of Belgrade, ³IGACC Belgrade, ⁴Faculty of agriculture, University of Belgrade, Serbia

The aim of this work was to investigate if the Comet assay could be used in the study of pre-manifested changes of cervix uteri epithelial cells. Squamous cells of cervix uteri were taken from 30 women in generative period, soaked into physiological solution and subjected to standard alkaline Comet procedure. Images of 50 randomly selected cells/patient were analyzed with Comet Assay IV Image Analysis system, PI, UK. In addition, Papanicolaou test (PAP) and colposcopic examination were performed for each patient. All patients had PAP 2nd group, but according to the colposcopic picture they could be divided into 3 groups: A - normal; B – atypical and C - normal, after conization or laser vaporization. Groups A and C had rare comets with significantly lower % of tail DNA comparing to group B. Interestingly, in A and C samples with large amount of lactobacilli comets could not be detected.According to obtained results, we can conclude that pre-manifested changes of squamous cervical cells include increased DNA damage that can be detected with Comet assay.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.109

INFECTIONS WITH HUMAN PAPILLOMA VIRUS (HPV) AND CYTOLOGIC CHANGES IN THE CERVICAL EPITHELIUM

Branković Ljiljana¹, Milićević Radomir¹, Živadinović Radomir² and Milašin Jelena ³

¹Clinic for Child Internal Diseases, Niš;²Clinicfor Gynecology and Obstetics, Niš

³Institute for Biology andHuman Genetics, School of Dentistry, University of Belgrade

The aims of the present study were: a) to establish the incidence of infections (HPV 16 and 18), b) to correlate viral infections with clinical and cytologic changes, c) the prognostic value of HPV infection assessment in terms of cervical cancer onset. 40 samples of cervical swabs have been subjected to PCR analysis. 17 patients were diagnosed as CIN I (42,5%), 5 CIN II (12,5%), 8 CIN III (20%) and 10 (25%) had cervical cancer. Viral infections were found in 67.5% of women with pathologic changes on their cervix (57.5% were positive for HPV 16, 25% for HPV 18, and out of the total number of cases, 15% were positive for both HPV 16 and 18). A high percentage of HPV infections has been detected in patients with cervical pathologies. HPV 16/18 have been found in all the analyzed groups with the highest incidence in patients with CIN II and CIN III. The number of infections detected in the group of younger patients with CIN I and older patients with carcinoma, is lower than expected, pointing most probably to infections with other types of HPV, not tested in this study. PCR proved to be a sensitive and reliable technique, easily applicable in HPV detection.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.112

DO U1 SMALL NUCLEAR RNA VARIANTS MEDIATE DIFFERENTIAL

PRE-mRNA SPLICING?

Herrera J. Rene, Somarelli A. Jason, Mesa Annia, Rodriguez E. Carol

Florida International University, College of Medicine, Miami, FL, USA.

The spliceosome is a RNA-protein complex responsible for excision of introns from pre-mRNA to produce mature RNA. This nucleo-protein complex is comprised of five small nuclear RNAs (snRNAs) as well as over 300 proteins. The snRNAs serve as scaffolds, onto which protein splicing factors bind, forming small nuclear ribonucleoprotein particles (snRNPs). Initiation of spliceosomal assembly onto the 5'exon/intron junction is performed by U1 snRNA, along with U1 snRNP proteins U1-70K, U1A, U1-C, the Sm core and other ubiquitous factors. The discovery of five major human U1 snRNA isoforms, with up to 28% sequence variability, has prompted our group to hypothesize that the nucleotide changes within protein binding sites may modulate differential formation of U1 snRNPs. It is also possible that the U1 snRNA variants enable the spliceosomal machinery to recognize variable splice site sequences. To test these hypotheses, 3-dimensional models of stem loop one and two were constructed for each U1 snRNA isoform, subsequently docked with U1-70K and U1-A, respectively. The results of these computations suggest that all variants interact with U1 snRNP proteins. To confirm this hypothesis,gel shift assays were first performed using ³²P labeled U1 variants and HeLa splicing extracts. Analyses of the spliceosomal assembly in native agarose gels indicate that all five U1 snRNA isoforms exhibit qualitative and quantitative differences in the formation of spliceosomal complexes. This experiment was followed by depletion studies from nuclear extracts and affinity binding assays of U1 specific proteins, suggesting that these complexes form U1-like snRNPs. The variability observed in the formation of spliceosomal complexes may provide insight into U1 snRNAs and/or proteins that may be involved in selection of variant splice sites playing a role in control of gene expression as well as development and tissue differentiation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.114

APPLICATION OF MOLECULAR MARKERS IN WHEAT BREEDING

Kobiljski Borislav, Kondić-Špika Ankica, Trkulja Dragana and Brbaklić Ljiljana

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Most recent advances in modern biotechnology in general and molecular markers in particular, have led to the development of a number of novel approaches that offer the chance of making plant breeding more efficient and more cost effective. This paper provides an overview of the current status in this respect, with special emphasis on the present process of integration of conventional and molecular breeding in the NS breeding programs. The molecular marker work in our Institute has few areas of interest: a) fragment analysis for the presence of major genes and loci linked to most important breeding traits of quantitative-poligenic origin in wheat (yield, abiotic and biotic stress and technological quality); b) DNA fingerprinting of 190 wheat varieties grown in R. of Serbia in last 40 years; C) validation of carefully chosen so called „candidate“ markers targeting evaluation of their application potential in NS wheat breeding program, and d) joint research-application work in the frame of international projects aiming further increase of potential for MAS (Marker Assited Selection) in wheat breeding.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.116

MOLECULAR DIVERSITY AMONG NATURAL POPULATIONS OF Lactobacillus paracasei AND Lactobacillus plantarum ISOLATED FROM DIFFERENT ECOLOGICAL NICHES

Golić Nataša, Terzić-Vidojević Amarela, Begović Jelena, Jovčić Branko, Veljović Katarina, Strahinić Ivana, Lozo Jelena, Nikolić Milica, Tolinački Maja and Topisirović Ljubiša

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

In the present study the genotypic diversity of more than hundred Lactobacillus paracasei and Lactobacillus plantarum strains isolated from different ecological niches including strains from goat’s and cow’s milk cheeses, human isolates and collection strains was investigated. Genotypic diversity was evidenced by rep-PCR fingerprinting using the (GTG)₅ primer. Genotypic characterization indicated a wide variability of the strains. Several clusters of strains were formed. In the first cluster were strains isolated from different cheeses and in fingerprint profile minimal differences among lactobacilli strains were observed. In the second group, there was a slight difference in the (GTG)₅-PCR pattern among lactobacilli strains isolated from various ecological niches. Finally, too many clusters of strains were formed with little or no information about their meaning in relation to the different sources of isolation. In conclusion, our data contribute to the description of microbial heterogeneity in lactobacilli. The results provide a more solid basis for understanding the functional and ecological significance of the presence of different lactobacilli biotypes in natural ecosystems.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.117

MOLECULAR CHARACTERIZATION OF BACTERIOCINS PRODUCED BY NATURAL ISOLATE Lactobacillus paracasei subsp. paracasei BGSJ2-8

Lozo Jelena, Kojić Milan, Jovčić Branko and Topisirović Ljubiša

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

Natural isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces two bacteriocins, BacSJ and acidocin 8912. Genes that encoded synthesis, maturation and export of the bacteriocins are located on the plasmid pSJ2-8. Sequencing and in silico analysis of the plasmid pSJ2-8 revealed 15 open reading frames (ORF). N-terminal sequence of the purified bacteriocin BacSJ, YSYFGGSNGY was used to determine ORF that encodes gene for bacteriocin production, bacSJ2-8. BacSJ is a 50 amino acid long peptide with the typical double-glycin leader consisting of 18 amino acids. The bacSJ2-8 gene is followed by immunity gene for BacSJ, bacSJ2-8i. Genes encoding ABC transporter and accessory protein necessary for maturation and export of both bacteriocins are also located on the plasmid pSJ2-8. Bacteriocin BacSJ is heat stable, active within broad pH range, protease sensitive and exhibits a narrow range of antimicrobial activity to the closely related bacteria. Biochemical and genetic analyses showed that BacSJ is a new bacteriocin that belongs to the class II bacteriocin.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.118

SECONDARY STRUCTURE WITHIN 5’ UNTRANSLATED REGION

OF THE kgmB mRNA

Vojnović Sandra, Ilić-Tomić Tatjana and Vasiljević Branka

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

The KgmB (kanamycin-gentamicin resistance) methylase from Streptomyces tenebrarius acts at G-1405 of 16S rRNA within the sequence CGUCA and thus protects the producer against its own toxic product. It was shown that the CGCCC pentanucleotide is present in the same region of 16S rRNA overleaping with the CGUCA sequence. Both pentanucleotides are also present in 82 nucleotides long 5’ UTR (5’ untranslated region) on kgmB mRNA in front of ribosome binding site (RBS), but in this case separated by xx nt. We proposed a model for translational autoregulation of KgmB methylase based on our in vivo studies (kgmB::lacZ gene and operon fusions). The model suggests that KgmB methylase recognizes the same sequences within 16S rRNA and 5’ UTR on its own mRNA. After methylation of all available target sites within 16S rRNA, KgmB is capable for preventing its own further translation. According to computer prediction of kgmB 5’ UTR CGCCC putative regulatory sequence is part of stable stem-loop structure. This prediction was tested in secondary structure probing experiments with different RNases. Experimental data and computational prediction of secondary structure will be presented.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.119

BIODEGRADATION OF ALKYL SULFATES

Jovčić Branko, Fira Đorđe, Topisirović Ljubiša and Kojić Milan

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

Interest in the microbial biodegradation of pollutants has intensified in recent years as humanity strives to find sustainable ways to cleanup contaminated environments. Long chain aliphatic sulfate esters such as sodium dodecyl sulfate (SDS) are in common use as components of surfactant formulations and are consequently discharged into wastewater. Previously was shown that Pseudomonas sp. ATCC19151 was able to grow on SDS as a sole carbon source and harbors a gene encoding a putative alkyl sulfatase (sdsA) and gene encoding its putative regulator (sdsB). We reported a growth ability of this strain in minimal media containing various concentrations of sodium dodecyl sulfate as a sole carbon source. The most prominent growth was detected for M9 minimal medium with 0.5% SDS (growth was detected even at 4% of SDS), thus this concentration of SDS was used for the monitoring of Pseudomonas sp. ATCC19151 SDS biodegradation dynamic by using MBAS assay. Bacterial growth coincided with the SDS disappearance, suggesting that Pseudomonas sp. ATCC19151 metabolise SDS efficiently.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.120

SENESCENCE-ASSOCIATED ASPARTIC PROTEINASE GENE FROM BUCKWHEAT

Timotijević Gordana, Milisavljević Mira, Samardžić Jelena,Konstantinović Miroslav and Maksimović Vesna

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

Analysis of aspartic protease (FeAP9) gene isolated from buckwheat gDNA showed that it contains 13 exons and 12 introns. Also, we have revealed presence of leader intron in 5’UTR of AP gen. Computer analysis of the leader intron predicted the existence of regulatory sequences that could be involved in responses to different hormonal and external stimuli which lead to senescence. Using Real-time PCR we found that the FeAP9 expression is upregulated in leaves under the influence of different abiotic stresses. RT PCR experiments using primers from 3'UTR indicate that FeAP9 was present in all analyzed tissues and it was differentially expressed comparing to another seed specific buckwheat AP: FeAP12. In situ RNA-RNA hybridization confirmed presence of FeAP9 mRNA in stem parenchyma cells as well as in cells of seed endosperm. Recombinant FeAP9 protein was produced using pMAL expression vector but protein was located mostly in inclusion bodies. Polyclonal antibody raised against oligopeptide corresponding to the N-terminus of FeAP9 was used in Western blot analysis. Protein of 47 kDa corresponding to FeAP9 was detected in all analyzed organs, while protein of 55 kDa was detected in flowers.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.121

UNIVERSAL AMPLIFICATION METHOD OF RETROTRANSPOSON TERMINAL LTR PARTS: NEW TOOL FOR POLYMORPHISM DETECTION IN PLANT AND ANIMAL GENOMES AND EFFICIENT RETROTRANSPOSON CLONING

Kalendar Ruslan

Institute of Biotechnology, University of Helsinki, Helsinki, Finland

Retrotransposons represent a highly abundant, ubiquitous class of repetitive elements widely dispersed in all eukaryotic genomes. These characters make them very attractive and useful for molecular marker development. Here, we describe a novel PCR-based method for the rapid isolation of LTR parts and entire retrotransposon sequences from any plant, animal or fungi genomes. This method allows discovering the sequences of LTR-retrotransposons not only belonging to described groups like gypsy, copia, LARD or TRIM but any families of LTR-retrotransposons. We present data for an efficient cloning of LTR sequences by using conservative retrotransposon sequences the only universal sequence present in all LTR-retrotransposons. This new technique is a powerful DNA fingerprinting technology applicable to plant and animal organism or stress activation cDNA transposon display without the need for prior sequence knowledge, also as novel specific isolation method for retrotransposon LTRs, with potential for cloning of LTR-retrotransposons and for study LTR-retrotransposons transcription assay with total RNA or for in-silico LTR-retrotransposons discovery. This method can in silico identify all types of LTR retrotransposons (gypsy, copia, LARD or TRIM). We tested this method on plant and animals genomes: Oryza sativa, Vitis vinifera, Arabidopsis thaliana, Solanum lycopersicum and Drosophila melanogaster.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.122

THE Rhox HOMEOBOX GENE FAMILY SHOWS SEXUALLY DIMORPHIC AND DYNAMIC EXPRESSION DURING MOUSE EMBRYONIC GONAD DEVELOPMENT

Daggag Hinda¹, Svingen Terje², Western Patrick ^1,4, van den Bergen Jocelyn^1,4, McClive Peter¹, Harley.Vincent³, Koopman Peter ^2,4 and Sinclair Andrew^1,4,*

¹Murdoch Children’s Research Institute and Dept. of Paediatrics, University of Melbourne, Royal Children’s Hospital, Melbourne, Australia, ²Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia,^3Prince Henry’s Institute for Medical Research, Monash Medical Centre, Clayton, Australia, ⁴Australian Research Council Centre of Excellence for Biotechnology and Development.

Reproductive capacity is fundamental to the survival of all species. Consequently, much research has been undertaken to better understand gametogenesis and the interplay between germ cells and the somatic cell lineages of the gonads. In this study, we have analyzed the embryonic expression pattern of the X-linked gene family Reproductive homeobox genes on the X chromosome (Rhox) in mice and report a germ cell-specific up-regulation of eight members during early gonadogenesis. We also show a sexually dimorphic and temporally dynamic expression pattern for several Rhox genes in the embryonic gonads. These changes coincide with critical stages of differentiation where the germ cells enter either mitotic arrest in the testis or meiotic arrest in the ovary. Finally, we show that Rhox8 (Tox) is the only member of the Rhox gene family that is expressed in the somatic compartment of the embryonic gonads. Our results indicate that the regulation of Rhox gene expression and their potential function during embryogenesis are quite distinct from those previously reported for Rhox gene regulation in post-natal gonads.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.123

CHOLESTEROL HOMEOSTASIS IN THE BRAIN- REGULATION OF GENE EXPRESSION IN AGING AND CALORIC RESTRICTION

Mladenović Djordjević Aleksandra, Perović Milka, Smiljanić Kosara, Tešić Vesna, Tanić Nikola, Ruždijić Sabera and Kanazir Selma

Laboratory for molecular neurobiology, Department for neurobiology, Institute for Biological Research “Sinisa Stanković”, Belgrade, Serbia

Holesterol is the main lipid constituent in the brain and important factor of brain plasticity. Disregulation of choleterol metabolism is underlying numerous brain disorders. In the brain cholesterol is synthesized in situ. Its homeostasis is maintained through precisely controlled processes of synthesis, degradation and export from the brain, were 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), cholesterol 24S-hydroxylase (CYP46) and apolipoprotein E (ApoE) respectively, are the key proteins.Expression profiles of cholesterol homeostasis genes were analyzed in the rat brain regions as a function of aging and in response to long-term dietary restriction (DR). Aging induced region specific changes in gene expression at both mRNA and protein levels for all genes examined. DR exhibited an attenuating effect on age-related changes through specific temporal and regional pattern with the tendency of reverting age-related changes at the control level. The importance of cholesterol brain homeostasis and the effect of DR on its maintenance point to potential therapeutic impact of DR on aging and age-related disease.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.124

THE ROLE OF microRNAs IN REGULATION OF BRCA1 GENE

Tanic Miljana, Zajac Magdalena, Benitez Javier and Martinez-Delgado Beatriz

Human Genetics Group, Spanish National Cancer Research Centre, Madrid, Spain

MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression by targeting the mRNAs 3’UTR and triggering either translational repression or RNA degradation. They have been implicated in an increasing number of biological processes, including tumorigenesis. Recent miRNA profiling studies have shown that miRNAs are deregulated in breast cancer. Breast cancer is a heterogeneous form of cancer and the central role of BRCA1 mutations in a number of both hereditary and sporadic breast tumors is well documented. Finding miRNAs that regulate the expression of BRCA1 might help explaining the development of tumor subtypes not accounted by mutations in known breast cancer susceptibility genes, and these miRNAs by themselves could infer susceptibility to breast cancer. In this study we have examined the expression of several miRNAs predicted to target BRCA1 3’UTR in both hereditary and sporadic breast cancer cell lines by means of QT-PCR. Functional studies have been performed in order to validate direct effect of these miRNAs on BRCA1 protein level via luciferasse reporter assay and western blotting.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.126

THE ESTIMATION OF PHYLOGENETIC RELATIONSHIPS AMONG THREE SPECIES OF GENUS Apodemus (Rodentia, Mammalia)

Bugarski-Stanojević Vanja, Stamenković Gorana, Adnađević Tanja, Blagojević Jelena,

and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

The faster evolving DNA characters are more informative for the estimation of phylogenetic relationships among very closely related species. Because of their exceptionally high mutation rate, microsatellites (SSR) may prove more useful than other DNA markers or sequence data. Evolutionary dynamics of the SSR loci may be evaluated by investigation of their structure, distribution and variability. The aim of our study was to investigate differences of their distribution in genomes of three species of the genus Apodemus (A.flavicollis, A. sylvaticus and A. agrarius). A total of 40 individuals from 7 localities were analysed by inter-simple sequence repeat (ISSR) technique, a SSR-primed PCR amplification, wich produces multilocus and highly polymorphous patterns. Each electrophoretic marker corresponds to a DNA sequence delimited by two inverted microsatellites. Out of twelve selected anchored and non-anchored primers with di- and trinucleotide repeats, six primers produced reproducible and polymorphic, species-specific paterns. These results indicate that ISSR markers are very useful tool for genetic characterization and phylogeny reconstruction for genus Apodemus species.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.127

MOLECULAR CHARACTERISATION OF SERATIA FONTICOLA

FROM OLIGOMINERAL WATER

Tasić Srđan¹ and Tasić Irena²

¹High school of applicable study, Vranje

²Faculty for informatic and managment, Belgrade, Serbia

In accordance with the Directive EU (80/777/EEC) special attention should be paid to determining the representatives of autochthonous bacteria species in a well in addition to the standard examination of the bacteriological content of natural mineral waters.The subject matter of molecular characterisation are isolates of oligomineral water on the Vlasina mountain which is used for the purpose of the water factory “Vlasinka ROSA”.The quality of the chromosomal DNA has been analysed on agarose gel. The isolated DNA has not been degraded. The PCR reaction was highly specific. The gene sequence for 16S rRNK from 1095 nucleotedes has been obtained. The sequencing of the amplified DNA has been performed according to Sanger.The comparison between the gene sequences with the NCBI data bank has been performed by means of the software ChromasPro, ver. 1.45. A similarity (98% of identity) of our strain with the strain Seratia fonticola LMG (gi|15054669|gb|AF286869.1) from from the Philippines isolated from the rice stems was determinet.The sequencing of 16S rDNA gene has proven to be more reliable than the use of the multitest identification systems (bioMèrieux) and the identification software (Apilab Plus V3.3.3.).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.128

MOLECULAR CHARACTERISATION OF Pseudomonas sp. FROM OLIGOMINERAL WATER

Tasić Srđan¹and Tasić Irena²

¹High school of applicable study, Vranje

²Faculty for informatic and managment, Belgrade, Serbia

The subject matter of molecular characterisation are isolates of oligomineral water on the Vlasina mountain which is used for the purpose of the water factory “Vlasinka ROSA”. The quality of the chromosomal DNA has been analysed on agarose gel. The isolated DNA has not been degraded. The PCR reaction was highly specific. The gene sequences for 16S rRNK from 1147 nucleotedes and 1154 nucleotedes have been obtained. The sequencing of the amplified DNA has been performed according to Sanger. The comparison between the gene sequences with the NCBI data bank has been performed by means of the software ChromasPro, ver. 1.45. A similarity (96% of identity) of our strain (1147 nucleotides) with the strain Pseudomonas putida from New Zealand marked as biotip A (gi|18076625|emb|AJ308311.1) was determined and it was used for the understanding of philogenical relationships. A similarity (99% of identity) of our strain (1154 nucelotides) with the strain Pseudomonas sp. marked as A-13 (gi|45454207|gb|AY556391.1) from China could be determined. Considering the identity percentage, the distance of the locality and the impossibility to identify the species, it may be concluded that it might be a ubiquitous species which has not been included in existing identification schemes because of its polimorphic biochemical characteristics.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.129

RAPD fingerprinting of indigenous Azotobacter isolates from different types of soil

Djuric Simonida¹, Jarak Mirjana¹ and Josic Dragana²

¹Faculty of agriculture, Novi Sad

²Institute of soil, Belgrade

Azotobacter is a genus of free-living diazotrophic bacteria whose resting stage is a cyst. It is primarily found in neutral to alkaline soils, in aquatic environments, in the plant rhizosphere and phyllosphere. Under extreme environmental conditions, the cell produced a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. Diazotrophic organisms such as Azotobacter play a vital role in every ecosystem, working to make nitrogen available to all organisms. It turns nitrogen into ammonia through the process of nitrogen fixation, after which the ammonia is turned into proteins. Unusual, when the cells reach stationary phase, the number of chromosomes can increase to 50-100 per cell, increasing the cell size dramatically. In our investigation, we compared RAPD pattern in the exponential growth phase of different Azotobacter isolates and their tolerance capability to herbicides. RAPD fingerprinting showed clear differences between the Azotobacter sp. isolates.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.130

Phosphate Solubilization and (GTG)₅ Analysis of Pseudomonads from rhizosphere

Jošić Dragana, Delić Dušica, Rasulić Nataša, Stajković Olivera,

Kuzmanović Đorđe and Miličić Bogić

Institute of soil, Belgrade

Plant hormone production, nutrient mobilization and plant protection are the generally accepted mechanisms for plant growth promotion. The rhizosphere of plants seems to be the most suitable place to search for these beneficial microorganisms since it’s a widely accepted fact that plants select those bacteria that may be more beneficial for their health. Pseudomonads are distributed widely in temperate and in tropical soils and often predominate among bacteria of plant rhizosphere. Specific strains of pseudomonads have the potential to enhance plant growth, suppress plant pathogens and participate in carbon and nitrogen cycling in nature. The role of pseudomonads in agriculture has been a matter of interest because of their abundant population in plant rhizosphere and their innate biocontrol properties.The present study revealed the genodiversity among pseudomonads of mayze rhizospheric soil. A screening for PGPRs (phosphate solubilization) was carried out. Results from rep-PCR fingerprinting using (GTG)₅ primer of phosphate-solubilizing strains indicated the low diversity existing in the selective environments.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.131

REP PCR AS A METHOD FOR DETERMINING DIVERSITIY AMONG Pseudomonas syringae STRAINS FROM FRUIT TREES

Ivanović Žarko¹, Gavrilović Veljko¹, Živković Svetlana¹and Stanković Slaviša²

¹Institute of plant protection and enviroment, Belgrade

²Faculty of Biology,University of Belgrade

Pseudomonas syringae is economically important plant pathogen, found on a number of hosts. This phytopathogenic bacteria is becoming a quite widespread pathogen on the fruit trees in Serbia, causing significant economic loses. Up to now it was experimentally confirmed as a pathogen on the pear, apple, peach, apricot, cherry, sour cherry, plum trees as well as raspberries. The genomic DNA fingerprinting technique known as repetitive sequence – based polymerase chain reaction (rep-PCR) was evaluated as a tool to differentiate Pseudomonas syringae strains isolated from the different fruit trees. BOX DNA primer, was used to generate genomic fingerprints. Genetic fingerprints were determined for 23 isolates of Pseudomonas syringae originated from peach, pear, apple, plum, sour cherry and raspberry using repetitive polymerase chain reaction (rep-PCR) based technique. Experiment demonstrates the potential of rep-PCR fingerprinting as a diagnostic tool in determining the genetic difference among the Pseudomonas syringae strains from various origin.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.132

CHARACTERIZATION OF THE METALLO-b-LACTAMASE GENE FROM A Pseudomonas aeruginosa CLINICAL ISOLATE

Lepšanović Zorica¹and Libisch Balázs²

¹Institute of epidemiology, Military Medical Academy, Belgrade

²Department for epidemiology, National center for epidemiology, Budampest, Hungary

Metallo-b-lactamases are emerging resistance determinants capable to hydrolyse both third-generation cephalosporins and carbapenems. The enzymes have been detected in multidrug-resistant nosocomial isolates of Pseudomonas aeruginosa and other Gram-negative pathogens.By the phenotypic tests, four P. aeruginosa clinical isolates obtained from Military Medical Academy proved MBL positive. PCR analysis demonstrated the presence of a bla_VIM gene. Molecular analysis of the first strains demonstrated that bla_VIM gene is integron-borne. Sequencing of the variable region of this integron revealed a bla_VIM cassette in the first position of the integron followed by orfD, a putative gene with unknown function. A strain was further characterised by multilocus sequence typing (MLST). The isolate displayed sequence type ST235 which belonged to international clonal complex CC11. These results confirmed an important role of clonal complex CC11 in dissemination of VIM type MBL, as well as marked heterogeneity of integrons carrying the MBL genes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.133

ANTIBIOTIC SUSCEPTIBILITY OF Staphylococcus epidermidis ISOLATED

FROM HUMAN MILK

Begović Jelena, Veljović Katarina, Strahinić Ivana and Topisirović Ljubiša

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

Staphylococci, particularly Staphylococcus epidermidis, seems to be the most predominant bacteria in human milk that represents an important source of staphylococci and other bacterial groups to the infant gut. All S. epidermidis isolates used in this study, originated from human breast milk and were identified according to 16S rDNA sequencing. The results obtained from rep-PCR analysis with three primer sets, (GTG)₅, ERIC and BOX revealed different strain types among tested isolates. In this study antimicrobial activities of 6 antibiotics against 14 isolates of S. epidermidis were investigated. Seven isolates were resistant to gentamycin (MIC≥32 mg/ml) and five were resistant to tetracycline (MIC=32 mg/ml). One isolate was multiresistant, i.e. resistant to four antibiotics, erythromycin, clindamycin, tetracycline and gentamycin. PCR analysis did not reveal the presence of three most commonly found methylase genes, ermA, ermB and ermC in this erythromycin resistant strain. On the other hand, PCR analysis with primers for genes encoding tetracycline efflux protein TetK and TetL revealed that all tetracycline resistant strains contained tetK gene. Computer-analyzed sequences revealed that they shared high nucleotid similarity (97-98%) with plasmid pT181 and TetK efflux protein from Staphylococcus aureus.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.134

XIIA group phytoplasma detection by RFLP analysis of 16s rDNA sequences

Jošić Dragana¹, Kuzmanović Slobodan², Stojanović Saša², Aleksić Goran²

and Starović Mira ²

Institute of soil, Belgrade

Institute of plant protection and enviroment, Belgrade

Phylogenetic analyses based on 16s rRNA of the uncultured phytoplasmas provided a basis for establishing a phylogenetically valid classification. PCR using phytoplasma group-specific or universal primers derived from conserved 16s rRNA gene sequences has provided a sensitive means for detection of a broad array of phytoplasmas from infected plants or insect vectors By direct sequence analysis or RFLP analysis of PCR-amplified 16s rDNA, the detected phytoplasmas can be differentiated and classified. Classification by RFLP analysis has provided a simple and rapid method that can be used to differentiate and identify a large number of unclarified phytoplasmas. In some cases, the phytoplasma groups classified on the basis of RFLP analyses using few restriction enzymes were not always consistent with groups based on phylogenetic analysis of 16s rRNA gene sequences. Our objective was to detect phytoplasmas of XIIA group based on RFLP analysis of 16s rDNA sequences using four restriction enzymes: MseI, AluI, KpnI and TaqI. We identified phytoplasmas of XIIA group in the three vinwyard regions (Zupa, Tules and Vrsac) using clean and specific nested-PCR products for RFLP analyses.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.135

ANTIMICROBIAL ACTIVITY OF NATURAL ISOLATES OF STRAIN Lactobacillus paracasei subsp. paracasei

Tolinački Maja, Fira Đorđe, Kojić Milan and Topisirović Ljubiša

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

According to our knowledge, only two bacteriocins from Lactobacillus paracasei subsp. paracasei isolates were purified and one was characterized on biochemical and genetical level. The first part of our research activities was the screening of natural isolates from the laboratory collection, classified as Lb. paracasei subsp. paracasei, for antimicrobial activity against relevant strains. These strains were isolated from homemade cheeses manufactured without addition of commercial starter cultures. Screening the collection resulted in identification of eight Lb. paracasei subsp. paracasei strains that produce bacteriocins. Analysis of antimicrobial spectrum and cross-immunity test between bacteriocin-producing strains indicated that they synthesize four different bacteriocins. Results of PCR experiments revealed that 22 of 32 analysed strains harbouring gene with 99% of identity with genetically characterized bacteriocin BacSJ. Among those strains, only two of them produced antimicrobial substances with identical antimicrobial spectrum as bacteriocin BacSJ. Also, results of PCR experiments revealed that 14 analysed strains showed 99% identity on nucleotide level with acidocin 8912.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.136

ANTIMICROBIAL AND PROTEOLYTIC ACTIVITY OF CHOSEN ENTEROCOCCI

Veljović Katarina, Terzić-Vidojević Amarela and Topisirović Ljubiša

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

A collection of 27 enterococci isolated from dairy and meat products was tested for antimicrobial and proteolytic activity as well as for susceptibility to antibiotic vancomycin. Eleven analysed enterococci produced antimicrobial compounds, while 10/11 enterococci synthesized enterocins. It was determined that 8/11 enterococci carried some of the genes encoding for enterocins A, B, P, 1071 and L50, except three isolates BGPT1-10P, BGPT1-78 and BG221. Moreover, E. faecalis BG221 showed antimicrobial activity that is not related to production of enterocin, H₂O₂ or organic acid, indicating that BG221 may be synthesized some antimicrobial metabolite. Proteolytic activity of enterococci was tested. Among them, five enterococci showed a good ability of β-casein degradation, while nine showed a weak proteolytic activity. Two enterococci, BGPT1-10P and BGPT1-78 hydrolysed α-, b-, κ-casein, total casein and gelatin. The molecular mass determination revealed that extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass about 29 kDa. Results showed that among tested enterococcal isolates, six were able to grow in the presence of vancomycin.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.137

MICROSATELLITE ALLELIC VARIABILITY IN Ambrosia artemisiifolia L. populations IN VOJVODINA

Kočiš Tubić Nataša, Obreht Dragana, Pal Boža and Vapa Ljiljana

Faculty of Science, University of Novi Sad, Novi Sad, Serbia

A. artemisifolia is the most frequent species of this genus in Europe and in Vojvodina. This species is also very abundant, widely distributed, dangerous invasive weed, and causes pollen alergies and contact allergies. The study described in this paper used microsatellite markers to evaluate the genetic variability in 3 natural populations collected in Vojvodina on 3 localities: Laćarak (Srem), Novi Sad (Bačka) and Đala (Banat). Microsatellite variability of four loci: Amb12, Amb16, Amb30 and Amb82 was analysed. All four loci given high level of polymorphism and they should be useful molecular markers for studying genetic variabillity of A. artemisiifolia populations. At loci Amb12 and Amb16 the lowest number of alleles (4) was detected, 11 alleles were identified at locus Amb30 and 8 alleles at locus Amb82. Expected heterozygosity was significantly higher than observed ones for all loci and populations. Genetic distance based on analysis of microsatellite variability, showed that population from Novi Sad of this species and population from Laćarak are geneticlly closer than population from Đala, and that can be caused by geographic barrier and low levels of gene flow.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.138

FUNCTIONAL PROMOTER ANALYSIS OF PLANT GENES IN BRIGHT YELLOW 2 CELLS AND POLLEN GRAINS

Bratić Ana^a,b, Majić Dragana^a, Samardžić Jelena^a, and Maksimović Vesna^a

Institute of Molecular Genetics and Genetic Engineering, Laboratory for plant molecular biology,Belgrade, Serbia

^a,bDivision of Metabolic Diseases, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden

Gene expression is mediated by DNA sequences directly upstream from the coding sequences by recruiting transcription factors, regulators and an RNA polymerase in a spatially defined fashion. Understanding promoter strength and the regulation mode would enhance our understanding of gene expression.The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay, in two transient expression systems: tobacco BY2 cells and pollen grains. Strong promoter activity was observed in both systems.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.139

TWO MITOCHONDRIAL GROUP II INTRONS IN Picea omorika: NEW INSIGHTS INTO EVOLUTIONARY RELATIONS AMONG SPRUCES

Aleksić Jelena*, Geburek Thomas, Fussi Barbara and Heinze Berthold

BFW – Austrian Federal Research Centre for Forests, Department of Genetics

Vienna, Austria

Close evolutionary relations between Picea omorika Panč. (Purk.), a Tertiary relict species endemic to the north-eastern Balkans, and two North American species P. mariana and P. rubens, argumented on the basis of crossability studies and supported by two recent comprehensive spruce molecular phylogenies, were tested using published data on two mitochondrial group II introns. Both introns - nad1i477, characterized by pronounced intra- and interspecific variability in spruces, and less variable nad5i230, revealed evolutionary relations among spruce species which were not concordant with two published spruce molecular phylogenies and crossability studies, but coincided with inferences based on morphological data reported more than 50 years ago. Nad1i477 enabled division of all spruces into two groups with essentially different organization and secondary structure of the domain IV, with P. omorika being placed into the group A, and P. mariana-rubens placed into the group B. The results are interpreted in relation to (i) the possible occurrence of hypervariability at nad1i477, and (ii) evolutionary relations of P. omorika and P. mariana-rubens, and (iii) the origin of spruces.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.140

CLONAL FIDELITY OF Iris sibirica PLANTLETS REGENERATED BY CULTURE IN VITRO

Jevremović Slađana, Stanišić Mariana, Ćalić-Dragosavac Dušica, Raspor Martin, Ninković Slavica, Subotić Angelina, Trifunović Milana and Petrić Marija

Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Genetic stability and uniformity of Iris sibirica plantlets regenerated by organogenesis and somatic embryogenesis, as well as, seedlings from natural populations, using flow-citometry and random amplified polymorphic DNA analysis (RAPD) were investigated. In vitro plant regeneration was induced in zygotic embryo culture on Murashige and Skoog nutritional media supplemented with 2, 4-dihlorophenoxy acetic acid (somatic embryogenesis) or thidiazuron (organogenesis). Plantlets derived by germination of somatic embryos or rooting of shoots were grown in greenhouse conditions one year before analysis. Plantlets regenerated by both processes have showed diploid number of chromosome using flow-citometry. Stability of the regenerated plants was screened by RAPD analysis using four decamer primers (OPD-08, OPD-11,OPD-13 and OPB-12). Among all tested plants, genetic variations were detected only within the group of plants obtained from seeds collected from nature.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.141

Investigations of genetic diversity of Paenibacillus larvae from Serbia using rep-PCR fingerprint technique

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Department for biology, Faculty of veterinary medicine, University of Belgrade

Paenibacillus larvae is the etiological agent of a severe disease called American foulbrood (AFB) which is lethal to infected honey bee larvae and can be devastating to colonies and apiaries. For diagnostic purposes genotyping of P. larvae using rep-PCR is very useful because of genotype-specific differences in virulence. Genotypes highly virulent at larval level (ERIC III and ERIC IV) are less virulent at colony level and are not expect to cause classical AFB-outbreaks. In contrast, P. larvae genotypes ERIC I and ERIC II are associated with classical AFB outbreaks. In this study, we investigated the diversity of P. larvae in Serbia. P. larvae isolates were obtained from broodcombs with or without clinical symptoms of AFB. Among 28 samples, 12 belonged to ERIC I (all from diseased brood), 5 to ERIC II (two from diseased and three from apparently health brood). The rest 11 isolates (all from colonies showing clinical symptoms) gave the patterns which were not assignable to any of published genotypes. The detection of new genotypes in Serbia represents the starting point for further investigation of their characteristics on the level of the pathogen as well as the bee colony.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.142

CHROMATIN MUTANTS AFFECTING PARAMUTATION OF r1

Kovačević Nives and Kaeppler Shawn

Plant Breeding and Plant Genetics Program, Department of Agronomy, University of Wisconsin, Madison, USA

Alternation of a chromatin state is achieved through interactions and modifications of DNA, RNA and chromatin associated proteins. Such complex, yet precise, interactions are utilized by living organisms to pack DNA and to regulate gene expression. Paramutation is an epigenetic phenomena that violates Mendel’s Second Law as one homologous DNA sequence alters the expression of the other in heritable a manner. Underlying mechanisms by which paramutagenic states are established remain largely uncharacterized. In our search to identify chromatin genes that affect paramutation initiation at the r1 locus in maize (which conditions aleurone pigmentation), we have screened 390 transgenic RNAi silencing lines targeting 100 different genes. Our search resulted in the identification of several genes that have a potential effect on paramutation of r1.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.143

STANDARDISATION OF METHODS FOR DETECTION OF VIRUS GENOME IN BLOOD AND BLOOD PRODUCTS: OUR EXPERIENCE WITH PARVOVIRUS B19

Nešić Milica¹, Kuzmanović Dragana¹, Jovanović-Ćupić Snežana², Libek Vesna³, Pašić Srđan⁴ and Stamenković Gorana⁵

¹Agency for drugs and medical supstances Serbia, National control laboratory, Belgrade; ²Institute of Nuclear Sciences VINČA , Laboratory for radiobiology and molecular genetics,Belgrade; ³ Clinical center – Zemun, Trasfusiology service, Belgrade, Serbia, ⁴Mother and Child Helthcare Institute of Serbia „Dr Vukan Čupić”, Belgrade; ⁵Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Human parvovirus B19 (B19), besides being implicated in mild fever, erythema infectiosum and arthropathy, may cause transient aplastic crisis, chronic anemia and fetal death. B19 is ubiquitous infectious agent resistant to most methods for virus inactivation in blood and blood products. Therefore, evaluation of B19 genome presence in these products by PCR is obligatory.Specificity of the primers for B19 genotype 1, the most frequent genotype in Europe, was evaluated by database analyses. The positive cut-off point was estimated using serial dilutions of B19 DNA BRP, and detection of 1 copy/μL was successful and repeatable. Positive and negative controls were used for method performance evaluation. In addition, internal control, artificially synthesized oligonucleotide amplified by the same B19 primers, was included in every step of the method.Using established PCR method we investigated plasma/blood samples of healthy blood donors, individuals with acute infection and immunocompromised patients. Results and the nature of B19 virus pointed out necessity of primers redesigning for less frequent genotypes 2 and 3 and upgrading our method to a quantitative one. The work is in progress.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.144

THE EVALUATION OF PCR METHOD FOR EARLY DETECTION AND MONITORING OF CITOMEGALOVIRUS INFECTION IN TRANSPLANTED PATIENTS

Savić Dejana, Kuljić Kapulica Nada and Jovanović Dragutin

Military Medical Academy, Belgrade

Cytomegalovirus (CMV) infection is an importante complication in transplanted patients and lead to serious disease in patients with decreased immunity.CMV DNA detection is made in plasma of patients with transplantation of stem cells or kidney. PCR comercial test Cobas Amlicor CMV Monitor Roche was applied. Monitoring CMV infection were performed in in the 82 post transplanted patients (33 recipients of kidney and 49 recipients of stem cells) during 2006.-2007.CMV DNA in blood of all post transplanted patients 18/82 was detected in 21.95%. From all of them were 8/33 (24.24%) recipients kidney and 10/49 (29.48%) recipients stem cells. Viral load in blood was between 5.37 x10² to 1.34 x 10⁵ cop/ml for hematology and 4.09 x 10² do 3.25 x 10³ cop/ml for nefrology patients.Viremia was absent in all cases after antiviral therapy with ganciclovir and valganciclovir.Quantification of CMV test is very important for early detection of CMV infection and monitoring of CMV DNA desease in patients after stem cells and kidney transplantation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.145

GM Arabidopsis thaliana FOR BIODETECTION AF LANDMINES

Meier Carsten¹ and Berenji Janoš²

¹Aresa, Coppenhagen, Denmark

²Field and vegetable crops Institute, Novi Sad

Arabidopsis thaliana (thale cress) has been genetically modified in order to use it for biodetection of landmines. The regulation of red pigment production (anthocyanins) has been engineered to cause the GM plant to respond to specific outer stimuli present in the environment, which results in a highly visible colour change of the plant from green to red within 3 weeks. Thus, the genetically engineered plants serve as a biodetection system able to detect landmines. The auter stimuli in this case is NO₂ cleaved from explosives. The efficiency of this this system has been demonstated under field conditions in 2007 and 2008.The reporter system can also be used in breeding of various crops (soybean, maize, cotton, canola, wheat, rice, etc.) for "reporting" the presence of specific genes.The patent application covering the technology responsible for the red colour formation in plants - as a ”Reporter System in Plants” was filed and published in December 2003 in the US, EU, Australia, New Zealand, South Africa, India, Singapore, China and Japan. Today the application has been accepted in New Zealand, Australia and EU.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.148

TRACES OF EVOLUTIONARY PROCESSES IN THE GENE POOL OF WESTERN-EURASIAN BEECHES

Gömöry Dušan and Paule Ladislav

Technical University in Zvolen, Slovakia

Common beech (Fagus sylvatica sensu lato) is an ideal object for the studies of microevolution. This taxonomic complex contains 4 taxa, however, their status is an issue of controversies. European beech (Fagus sylvatica L. ssp. sylvatica) is a generally recognized species with a wide European range. Eastern beech (Fagus sylvatica ssp. orientalis Lipsky) is sometimes considered a separate species, distributed over northern Asia Minor, the Caucasus and the Alborz Mts., with isolated occurrences in eastern Bulgaria and Greece. Two additional taxa, Balkan beech (F. moesiaca Czeczott) and Crimean beech (F. taurica Popl.) are mostly regarded as transitions between both main taxa.Compared to the diversity of the fossil record, the recent beech exhibits a strange morphological homogeneity over its European range. F. sylvatica s.s. survived the last glaciation in small refugial populations located at the tree southern peninsulas and there is paleobotanic as well as genetic evidence that it spread into most of its present range from a single refugium located in southeastern Alps. This colonization process is reflected in the present trends of genetic variation: allelic richness decreases from the refugia towards the marginal populations, whereas gene diversity exhibits just opposite trend. This pattern apparently results from repeated founding events during the recolonization.Beech in the southwestern part of the Balkans differs from the remaining European populations both in chloroplast and nuclear genes. However, the genetic transition from the Slovenian to Bulgarian populations is smooth. This cline is results from isolation by distance rather that adaptation. The absence of a distinct boundary does not support the existence of a separate taxon in this area.The range of Eastern beech is much more fragmented than that in Europe. The long-term geographical isolation, maybe lasting since the Tertiary, lead to the formation of three distinct gene pools, represented in different areas: western Asia Minor, the Caucasus and the Alborz. The populations in eastern Asia Minor, the Amanus Mts. and the Crimea contain a mixture of essentially the Turkish and the Caucasian gene pools. Beech in western Turkey is actually les differentiated from European beech than from the other Eastern beech populations. European beech appears thus as a paraphyletic taxon. This is a further argument to support pooling all western-Eurasian populations into a composite species Fagus sylvatica with two subspecies.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.149

FOREST MOLECULAR GENETICS: PROMISES, BREAKTHROUGHS, AND THE PRACTICE

Heinze Berthold

Federal Research Centre for Forests, Department of Genetics, Vienna, Austria

Forest genetics has gone a long way, from observation of differences between provenances within species, altitudinal races in mountainous regions, or clones of vegetatively propagated trees. Over the decades, single genes with visible effects (mostly deficiency mutants), metabolites as markers, isoenzymes (also called allozymes), and various kinds of DNA or protein-based markers have allowed ever increasing insights into the tree genome, culminating in the publication of the first fully sequenced genome of a Populus tree in 2006. In retrospect, almost every one of these technological breakthroughs has been accompanied by great expectations and promises of new applications based upon the new findings. In a review that contrasts the expectations, in their historical context, with real changes in forestry practice, I will try to identify the major obstacles that have hindered more widespread application of modern genetic knowledge: is it foresters being more conservative, scientists promising more than they can deliver, a problem of state-of-the-art being transferred into products on a grand scale, or are we alre

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.152

NEW TRENDS IN PLANT BREEDING

Janoš Berenji

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Molecular breeding. Advances in biotechnology provide plant breeders with knowledge and tools to produce "designer crops" like transgenic crops in order to fulfill special needs, particularly tolerance to abiotic and biotic stresses, improved quality, etc.

Breeding for global climate change. The global climate change is expected to result temperature change, altered rainfall patterns and other direct or indirect effects on crops. It is important to prepare the desired kinds of new varieties to have them at disposal when predicted climate change effects will be experienced more consistently.

Breeding Bioenergy Crops. Breeding bioenergy crops frequently deals with conventional food crops like maize, wheat or sugar beet for ethanol or oil crops for biodiesel. Sorghum (Sorghum bicolor) used for bioalcohol or jatropa (Jatropha curcas) for biodiesel production are among the examples of dedicated bioenergy crops.

Organic plant breeding. Organic plant breeding as a new trend in plant breeding with new approaches, breeding goals and methods used to bred organic cultivars specially adapted to organic agriculture. Participatory plant breeding might play a significant role in organic plant breeding.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.153

THE USE OF GENETIC MARKERS IN THE ENHANCEMENT

OF FOREST TREES SEED PRODUCTION IN SERBIA

Isajev Vasilije¹, Orlović Saša², Konstantinov Kosana³, Drinić Mladenović Snežana³, Lučić Aleksandar⁴and Ivetić Vladan¹

¹Forestry faculty, Belgrade, Serbia,

²Institute of Lowland Forestry and Environment, Novi Sad

³Maize research Institute «Zemun Polje», Belgrade-Zemun,

⁴Institute for Forestry, Belgrade, Serbia

The paper presents the results of the morphological and genetic marker application in the enhancement of forest trees seed production by the designation of regions of Austrian pine, Norway spruce, beech provenances in Serbia. The genetic specificities of these species in Serbia were studied by the analysis of genome structure of their seed stands, by using protein and PCR based markers. The research of the genetic structure of mentioned species by using genetic markers was the base of study :-the degree of variability of cone, seed and fruit morphometric characteristics and the interdependence of the analysed characteristics;-genetic similarity by the analysis of genetic markers;-the relationship of ecological attributes characterising the site from which the analysed seeds and fruits originate and the results of laboratory analyses.The final regionalisation of seed stands should be based on two aspects – genetic differentiation and gene pool conservation of those species (in-situ and ex-situ), in order to protect the genetic specificities of their natural populations in Serbia

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.154

BIOTECHNOLOGY AND GENETIC IMPROVEMENT OF DOMESTIC ANIMALS

Miščević Branislav, Bošković Jelena, Ivanc Aleksandar, Vukosav Marija and Kenjveš Tibor

Faculty of biofarming, Bačka Topola

Biotechnology and genetic improvement are the key point for efficient increasing of farm animals number, quality improvement and quantity of products such as meat, milk and eggs. Genetic improvement and reproduction are basic prerequisites for successful animal production and also very important elements in animal biotechnological research. New production techniques are developed and applied in praxis. These techniques are specific and mark the development of this century: Artificial insemination and semen deep freezing, Apply of embryo transfer and manipulation with embryos,Sexing of semen and embryos, Gene cloning and creating functional genetic constructions, Gene mapping,Producing transgenic animals.

Developed techniques are used for certain goals in animal husbandry:Advantages of frozen semen and embryos in changing of genetic material world wide, Advantages in using hygiene concepts using AI and ET, Improvement of genetic progress based on biotechnology, Improvement and even obtaining completely new products from domestic animals.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.155

MAS OF SUNFLOWER FOR THE RESISTANCE TO DOWNY MILDEW

Saftić-Panković Dejana, Radovanović Nataša, Jocić Siniša

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Sunflower downy mildew is a disease caused by the oomycetes of Plasmopara halstedii. Dominant gene Pl6 confers resistance to four races of P. halstedii which are the most abundoned in Europe. New sunflower lines resistant to downy mildew are produced by implementation of Pl6 gene with marker assisted selection (MAS). Pl6 is positioned on linkage group 8 of the SSR map, together with several dominant and co-dominant markers for downy mildew resistance. The evaluation of molecular markers for identification of resistant plants across a diverse range of sunflower germplasm enabled us to define one dominant and one co-dominant CAPS marker, as the most useful for efficient MAS in a breeding program. The expression of Pl6 gene in young sunflower leaves was also investigated. Obtained results indicate that Pl6 gene is constitutive. Fragments amplified on cDNA from resistant and susceptible line are of same size but differ in sequence and the level of expression. Additional experiments which are neccessary to explain the difference in Pl6 gene expression between resistant and susceptible line, will also give valuable information for MAS of downy mildew resistant sunflower plants.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.156

Parentage Verification and sex determination in cattle using molecular markers

Stevanović Jevrosima, Stanimirović Zoran, Ninoslav Đelić, Radaković Milena

Department for biology, Faculty of veterinary medicine, University of Belgrade

Parentage verification in cattle using molecular markers is necessary for each sire and dam in genetic improvement programs. A certificate of parentage testing, issued on the basis of microsatellite analysis, is requested for each sire and dam included in artificial insemination program. These facts led us to introduce the method for parentage verification in cattle using informative set of 11 microsatellite markers recommended by the Cattle Molecular Markers and Parentage Testing Standing Committee of the International Society of Animal Genetics (ISAG) including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225 and BM1824. Certificate and passport needed for international cattle movement contain parentage data obtained using the listed microsatellite markers. Fetal sex determination in cattle is important because EU export and import of beef carries a subsidy difference depending on whether the beef is declared as male (considered to be higher quality) or female. Bovine amelogenin gene (AMX/Y) residing on the sex chromosomes is molecular marker for prenatal fetal sex determination. The same marker is used for the PCR-based diagnosis of freemartinism.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.157

MAIZE BREEDING TOWARDS DROUGHT TOLERANCE

Anđelković Violeta, Marković Ksenija, Ignjatović-Micić Dragana, Nikolić Ana, Drinić Mladenović Snežana and Lazić-Jančić Vesna

Maize Research Institute Zemun Polje, Belgrade, Serbia

It is expected that global warming will induce climatic changes, drought escalation and increase in crops water needs. Maize, which is the third world crop by acreage and usage, is the plant with yielding capacity highly influenced by drought.The scientific programs toward improvement of existing maize germplasm and development of drought tolerant hybrids with stable yield under water deficit were initiated in Maize Research Institute „Zemun Polje” during early seventies of the 20th century. These programs were evolving and changing together with the new scientific discoveries and technical developments. Through the years improvement of maize tolerance to drought was achieved by the methods of conventional breeding, based solely on phenotypic expression of favorable traits. Contemporary programs aim the increased implementation of molecular marker techniques in various stages of corn breeding.The most relevant results of these programs will be discussed in the presentation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.158

STATE AND PERSPECTIVE OF PLANT GENETIC RESOURCES IN SERBIA

Boćanski Jan and Savić Ivanov Milena

Ministry of Agriculture, Forestry and Water Management of Republic of Serbia, Plant Protection Directorate, Novi Beograd

Plant genetic resource is each plant genetic material which has actual, or potential value for food, or other use in agriculture. Natural privileges of our climate bring huge resources and numerous plant species that are important genetic resources for food and agriculture, as well as for economic development of the country. Plant genetic resources are necessary support for genetic improvement of crops, whether by breeding, or modern biotechnology. They are also of vital importance for adaption to unpredictable ecological changes and future human needs. Various resistance to diseases, stress conditions, and pests present in wild relatives, old varieties and populations could be vital factor in plant production development. Plant genetic resources are endangered directly (urbanization, development and expansion of cities) and indirectly ( contamination of water, ground and air), as well as by other factors (climate changes, natural disasters, introduction of foreign organisms). Collections of plant genetic resources of fruit, grape-vine, vegetable, cereals, industrial, fodder plants, drug plants are stored in institutes and faculties in Serbia, and there is a project for establishment Plant Gene Bank as national institution.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.159

CROP, FORESTRY AND ANIMAL GENETIC RESOURCES – ROLE OF BIOTECHNOLOGY FOR THE CHARACTERIZATION AND CONSERVATION

KonstantinovKosana^{1, 2}, Drinić Mladenović Snežana¹ and IsajevVasilije³

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

² Academy of engineering sciencies of Serbia, Belgrade, Serbia

³Faculty of forest, university of Belgrade, Serbia

There are different levels of genetic diversity, including ecosystems communities, populations, genotypes and individual genes. There are tremendous technical possibilities available to create new varieties, improve efficiencies, and increase value of the genotypes of interest. Central to sustainable conservation is the knowledge of the genetic diversity present in a genebank. The effective use of genetic reources stored in genebanks by breeding programs is limited. Germplasm characterization has been developed mostly on classic descriptors (traits) and at present more and more molecular biotechnology application. Advances in molecular genetics have allowed numerous useful genes to be introduced, identified and used as markers in genotypes characterization. Interspecific hybridization has been used to develop some animals that are useful for aquaculture but at present there are trans- genetic aquatic species available to the consumer. Conservation genetics of forest tree species takes advantage from the availability of molecular biotechnology. Molecular characterization is helpful to make informed decisions on the conservation of genetic resources.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.162

phenotypic reaction of wheat grown on different soil types

Dimitrijević Miodrag, Petrović Sofija, Banjac Borislav and Vukosavljev Mirjana

University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Phenotypic reaction of eight wheat varieties under different growing conditions was followed. Trials were conducted at localities of Kumane and Rimski Šančevi on solonetz, humoglay and black soil. The yield, as well as, the yield components plant height, spike length and harvest index were studied. The effect of different environments on varietal phenotypic variability, with the emphasis on genotype by environment interaction, was quantified and analyzed using AMMI model.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.163

IMPACT OF GLOBAL AND REGIONAL CLIMATE CHANGES ON MAIZE GRAIN YIELD

Šurlan-Momirović Gordana¹, Zorić Miroslav¹,Kovačević Vlado², Prodanović Slaven¹, Vučković Savo¹, Maklenović Violeta³,Jolanka Marton⁴ and Birkas Marta⁴

¹ Faculty of Agriculture, Belgrade-Zemun

²University J. J. Strossmayer – Poljoprivredni fakultet, Osijek, Hrvatska

³Institute for agriculture“Agrorazvoj”, Niš

⁴Szent Istvan University - Institute of Crop Production, Gödöllö, Hungary

A constant growth of a human population needs to be followed by increasing of food production. However, climate changes observed during last decades affected decrease of the yield in major field crops, such as maize. Different type of researches which explore links between climate change and agricultural adoption and mitigation options are urgently needed. In this research, we studied how environmental changes in region of Balkan affected the maize grain yield during the ten years period (1996-2007). Data were collected from eight locations (Niš, Novi Sad, Osijek, Zagreb, Vukovar, Virovitica, Bekes, Fejer) in three countries (Serbia, Croatia and Hungary). Different statistical methods (ANOVA, correlations, stepwise regression analysis and pairwise association) for estimation of relationships between environmental variables (precipitation and air-temperature) and maize grain yield were used. Obtained results at local level were compared with other similar investigation on the world.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.164

ANALYSIS OF INFLUENCE OF PARENTAL GERMPLASM ON FRUITS CHARACTERS OF F₁, F₂ AND F₃ GENERATIONS OF PEPPER HYBRIDS (Capsicum annuum L.)

Zečević Bogoljub¹, Đorđević Radiša¹, Damnjanović Jelena¹, Đorđević Mladen¹, Todorović Goran² and Jevđović Radosav²

¹Institute for Vegetable Crops Smederevska Palanka

²Institute for Medicinal Plants Research "Dr.Josif Pančić" Belgrade

The aim of this research was to determine genetic values for the fruit width and fruit length in F₁and F₂ generation of diallely crossed pepper genotypes, and to evaluate the effects of applying the pedigree and bulk breeding method for producing F₃ hybrid populations. Hybrid populations were formed by crossing divergent genotypes belonging to different varieties of species Capsicum annuum L. The research of parent lines lasted for three years, experiments with F₁ generation two years, while the analysis of results for F₃ hybrid population has been done regarding the data from one year experiment. The heterosis effect in F₁ generation for all researched traits and in all crossing combinations was very low. Genetic analysis of F₂ generation showed that all researched traits were inherited with partial domination. In F₃ generation, hybrid populations formed by using the pedigree method of selection had traits which are more valuable for further breeding than the hybrid population formed by using modified bulk method.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.165

LEAF TYPES IN LEGUMES AND THEIR AGRONOMIC IMPORTANCE

Ellis Noel¹, Hofer Julie¹, Mikić Aleksandar², Mihailović Vojislav², Vasiljević Sanja², Milić Dragan², Đorđević Vuk²and Perić Vesna³

¹John Innes Centre, Department of Crop Genetics, Colney Lane, Norwich,UK

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Maize Reseearch Institute Zemun Polje, Zemun, Serbia

The majority of legume species have compound leaves with leaflet pairs arranged along the rachis that are subtended by a proximal pair of stipules. Within the Cicereae and Fabeae the distal organs can be tendrils rather than leaflets. Formation of these leaf organs in diverse species is under the control of several genes. The greatest collection of leaf organ variation in an economically important legume is in pea. The pea afila mutants, with the a genetic structure of afaf TlTl, have leaves that are a branched system of tendrils and this variant is widely regarded as the greatest innovation in pea breeding in the last 20 years. This has led to the development of the varieties with a significantly improved standing ability and reduced grain yield losses. A pea mutant with all tendrils transformed into leaflets, called acacia and with a genetic structure of AfAf tltl, is considered to have a great potential for forage production, as are genotypes of lucerne (alfalfa) and clovers with 5 or 7 leaflets instead of the standard 3. More than 3 leaflets in soybean do not necessarily lead to higher grain yields, but improves its potential for forage and biomass production.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.166

'BEAUTIFUL VAVILOVIA' (Vavilovia formosa) AND MOLECULAR TAXONOMY OF TRIBE Fabeae

Smýkall Petrl¹, Kenicer Greg² and Mikić Aleksandar³

¹Agritec Plant Research Ltd., Šumperk, Czech Republic

²Royal Botanic Garden Edinburgh, Edinburgh, UK

³Institute of Field and Vegetable Crops, Novi Sad, Serbia

Tribe Fabeae (formerly Vicieae) contains five genera, including Lathyrus (vetchlings), Lens (lentils), Pisum (peas) and Vicia (vetches). The remaining monotypic genus, Vavilovia, is obscure and intriguing. Its only species, 'beautiful vavilovia' (Vavilovia formosa (Steven) Fed.), is a perennial found in the Caucasus, eastern Turkey, northern parts of Iraq, Iran, Syria and Lebanon. Among the traits that support a closer relationship of Vavilovia are susceptibility to the pea-specific fungi Uromyces pisi and Ascochyta pisi, and the possibility of mutual hybridization. Until now molecular phylogenetics has not been used to determine anything regarding Vavilovia's relationship to Pisum and Lathyrus. Using sequences of four phylogenetically informative regions; the maturase K, trn L-F and trn S-G spacer regions of chloroplast DNA, and the internal transcribed spacer (ITS) region of nuclear DNA in 30 species of tribe Fabeae we investigated the relationships among the genera. Maximum parsimony and Bayesian analysis of combined sequence data confirmed Vavilovia as a distinct group within a Lathyrus – Pisum - Vavilovia clade.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.167

PROMISING SOUR CHERRY HYBRIDS (Prunus cerasus L.) DEVELOPED

AT FRUIT RESEARCH INSTITUTE ČAČAK

Radičević Sanja, Cerović Radosav, Glišić Ivana and Karaklajić-Stajić Žaklina

Fruit research institute, Čačak

At Fruit Research Institute Čačak, some of the major objectives of the breeding work on developing sour cherry (Prunus cerasus L.) cultivars are high cropping, large and high-quality fruits and resistance to causal agents of the most serious diseases and pests.

The paper presents two-year results of the study of pomological properties, biochemical composition of fruits and resistance under field conditions of the four promising sour cherry hybrids, i.e. III/23 (‘Köröser Weichsel’ x ‘Heimanns Konserven Weichsel’), III/31 (‘Köröser Weichsel’ x ‘Heimanns Rubin’), II/40 (‘Köröser Weichsel’ x ‘Heimanns Konserven Weichsel’) and XII/57 (‘Čačanski Rubin’ x ‘Heimanns Konserven Weichsel’) all of which were compared with standard cultivar ‘Heimanns Konserven Weichsel’.

As compared to standard cultivar, these hybrids are characterised by higher fruit weight and more favourable biochemical composition of fruits. In addition, they demonstrate considerably higher resistance to Monilia sp., manifested on shoots and fruits, cherry leaf spot (Blumeriella jaapii (Rehm.) v. Arx.), shot hole in sour and sweet cherry (Clasterosporium carpophilum (Lév.) Aderh.) as well as cherry fruit fly (Rhagoletis cerasi L.).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.168

Scots pine (Pinus silvestris L.) Ecotypes of the illyrian region in the afforestation of sites in south-eastern Serbia

Rakonjac Ljubinko¹, Isajev Vasilije² and Lučić Aleksandar

¹Institute of forestry, Belgrad, ²Forestry faculty, Belgrade

The methodological approach to Scots pine ecotype differentiation was studied. It was presented that the specific site conditions of the wider ecological range of Scots pine in Bosnia could have affected the processes of the reproductive isolation of this species, despite the small geographic distance of the study populations. The study of Scots pine ecotype differentiation shows that there is a regular interdependence between Scots pine morphological-anatomical and eco-physiological properties and the characters of the sites. It is the defined regularities of the distribution of the researched elements by the study groups that actually represent the adopted parameters of Scots pine ecotype differentiation. Based on the analysis of all differential characters, the populations are grouped into 5 Scots pine ecotypes.The planting stock applied in the afforestation with Scots pine in the conditions of south-eastern Serbia should originate from the phenogroup B1 (after Tošić’s classification of the sites very close to Scots pine natural sites - regarding the altitude).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.169

USING OF GEOGRAPHICAL COMPONENT OF GENETIC DATA

Ivetić Vladan¹, Isajev Vasilije¹ and Drinić Mladenović Snežana²

¹Forestry faculty, Belgrad

²Maize Research Institute Zemun Polje, Belgrade, Serbia

Genetic diversity can be spatially differentiated on different levels, like populations, subpopulations and between neighboring individuals. This spatial distribution is always caused by influences from outside, like human activities, phase of life development and demographic history of species. Because of that, knowing of spatial genetic structure provide useful tool in researching of these causal factors and for better understanding of genetic processes like differential selective pressure, gene flow and genetic drift. Unlike estimators of population genetic, which demands averaging inside populations or making hierarchical models, spatial autocorrelation use data from all individual pairs from sample plot, and by that gives much useful information’s on population level. In this paper, use of several methods for investigating of spatial genetic structure is presented, on example of genetic data derived from natural populations of beech, by using of RAPD markers, from spatial autocorrelation, Mantel test, interpolation of genetic data, to use of Monmonier’s algorithm for making genetic barriers.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.170

INTERSPECIFIC DNA VARIATION IN POPLARS USING AFLP AND SSR MARKERS

Galović Vladislava, Orlović Saša, Kovačević Branislav and Pilipović Andrej

Institute of Lowland Forestry and Environment, Novi Sad, Serbia

The objective of this paper was to examine DNA variation in poplars using AFLP and SSR markers in order to establish a fingerprinting profiles starting with germplasm (different genetic background) of the core collection of the Institute of Lowland Forestry and Environment, Novi Sad, Serbia. The markers polymorphisms and its power of discrimination for AFLP and SSR, respectively, were determined within 13 clones of two sections (Aigeiros and Tacamahaca) of genus Populus. Twelve sets of microsatellite primers, and 5 AFLP primer combinations were chosen for this study. Out of 12 SSR markers 10 were successfully amplified. Out of 12 loci, PTR1, PTR3, PTR6, and PTR14 were the most polymorphic. Power of discrimination for all SSR markers ranged from 0,13 – 0,81. Out of five AFLP primer combinations Pes1/Pms1 reveal the most polymorphic pattern. Power of discrimination for AFLP markers ranged from 0,82 – 0,92. When evaluating polymorphisms within clones of interest microsatellite and AFLP DNA markers could be successfully used in distinguishing tree clones, and thus revealing the fingerprinting pattern. It could be of significant help in poplar breeding programs and could also be used as a valuable annex in registration process to check for trueness to type of new bred clones and also facilitate the protection of plant breeders rights.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.171

Species identification of Nosema microsporidian pathogen in samples of Apis mellifera from Serbia using PCR-RFLP method

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Department for biology, Faculty of veterinary medicine, University of Belgrade

Honey bee Apis mellifera, was formerly considered to be parasitized by one microsporidian, Nosema apis. In recent years, the presence of new parasite Nosema ceranae has been detected in A. mellifera worldwide (Europe, Asia, United States and Canada). During the same period, honey bees have been strangely disappearing from their hives, and strong colonies have suddenly become weak and died (colony collaps disorder-CCD). Such symptoms have been also reported by beekeepers in Serbia during last two years, indicating a need for studies on the epidemiology of N. ceranae in A. mellifera on the territory of Serbia. from Adult foraging bees were sampled from 144 colonies (from 112 locations throughout Serbia) with or without apparent nosema disease. Only Nosema-positive samples (n=84, confirmed by light microscopy examination) were analysed with PCR–RFLP method to distinguish between N. ceranae and N. apis. All samples gave RFLP pattern characteristic for N. ceranae. The possibility of causative association between the colonization of Apis mellifera by N. ceranae and severe bee losses in Serbia is discussed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.172

GENETIC MAPPING OF LEAF RUST (Puccinia hordei Otth) Resistance In Barley Accession MBR1012 ORIGINATING FROM R. OF SERBIA AND R. OF MONTENEGRO

Perović D.¹, Günter J.¹, Steffenson B.², Kopahnke D.¹, Pržulj Novo³ and Ordon F.¹

¹Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany;

²Department of Plant Pathology, University of Minnesota, St. Paul, USA

³ Institute of Field and Vegetable Crops, Novi Sad, Serbia

To promote the effective use of barley landraces in breeding programs, they need to be fully characterised and evaluated for agronomical traits. Characterization of such genetic resources has to give special attention to disease resistance as the gene-pool of cultivated barley is largely depleted of major resistance genes for many plant pathogens, e.g. leaf rust. After detection of resistance to the available virulent isolates of pathogens, it is necessary to genetically map the corresponding genes in order to effectively incorporate them into adapted breeding lines. Here we present preliminary results on the mapping of resistance to leaf rust (Puccinia hordei Otth) isolates I80 and I90 detected in the landrace MBR1012 from Serbia and Montenegro. Mapping revealed the localization at two new positions on the short arm of chromosome 1H indicating two new resistance genes. Next, the relation to Rph4, which is also located on chromosome 1H has to be analysed by tests for allelism. Further characterization and utilisation of these genes is discussed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.173

THE DROUGHT RESPONCE OF WHEAT LANDRACES FROM THE WESTERN BALKAN

Dodig Dejan¹, Kandić Vesna¹, Zorić Miroslav², Šurlan-Momirović Gordana² and Knežević Desimir³

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²Faculty of Agriculture, Belgrade-Zemun

³Faculty of Agriculture, University of Pristina, Zubin Potok, Serbia

Landraces of common wheat, collected from the Western Balkans, were considered as a potential genetic resource of drought resistance for wheat breeding. A group of 20 landraces with 80 modern and obsolete wheat cultivars of worldwide origin were tested in a 4-year field trials under two watering regimes: fully irrigated and in a rain-out plot shelter. Three selection indices, including mean productivity (MP), tolerance (TOL), and stress susceptibility index (SSI) were calculated based on grain yield in irrigated and drought-stressed conditions. The average yield of landraces was significantly lower than the average yield of cultivars under both irrigated and drought stress conditions. Beside, there was no evidence that yield stability of landraces was better than in cultivars, as expected. On the other hand, average minimum yield decrease under stress with respect to favourable conditions (TOL) was significantly lower in landraces than in cultivars. Nevertheless, selection for drought tolerance can cause reduction in MP, which is of first choice considering drought indices as a selection criterion for the variable rainfed conditions of south-eastern Europe. Based on obtained results we have concluded that studied landraces is of little value for improving yield and its stability of new varieties for the regional market.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.174

QTL ANALYSIS OF TRAITS CONTRIBUTING TO DROUGHT TOLERANCE IN MAIZE

Nikolić Ana¹, Ignjatović-Micić Dragana¹, Lazić-Jančić Vesna¹ and Pekić Sofija²

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

² Faculty of Agriculture, Belgrade-Zemun

Drought is one of the most important factors limiting maize production all over the world, thus development of drought-tolerant maize varieties is of high importance. Drought tolerance is hard to achieve through conventional breeding because of its complexity. Understanding the genetic basis of this trait has a significant influence on maize productivity. Phenotypic data in combination with genetic marker data allow mapping quantitative trait loci (QTLs) involved in the expression of the trait. Identification of QTLs was done on F2/ F3 populations from a cross between drought tolerant (DTP79) and susceptible (B73) maize lines, will be presented. F₂segregating population was used for linkage map construction based on molecular markers (RFLP, AFLP, SSR). F2 plants were selfed, and F3 families were used for phenotypic traits (yield, yield components, morphological and physiological traits) evaluation. QTL Cartographer V 2-5 was used for QTL mapping.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.175

TRANSMISSION OF RESISTANCE TO TOBACCO MOSAIC VIRUS (TMV) IN SOME ORIENTAL TOBACCO VARIETIES

Dimitrieski Miroslav

Tobacco Institute-Prilep, Macedonia

The aim of investigation was to study the transmission of TMV resistance in some commercial varieties of oriental tobacco by the method of backcross hybridization. Investigation included resistant introduced oriental varieties (AA) and one susceptible variety (aa). In relation to other qualitative and productional characteristics, the latter variety was good for the purchasers. In F1, resistant heterozygous progeny (Aa) was obtained, which was back pollinated with pollen of the recipient variety(aa). In BC1, as well as in other generations up to BC5 and BC6, resistant plants, phenotypically more similar to the recipient variety, were pollinated again with the pollen of this variety. Infective juice from diseased plants was used for inoculation of plants from hybrid generations, using the method of Ternovskiy.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.176

ROLE OF GENETIC RESOURCES OF DIFFERENT GEOGRAPHIC ORIGIN IN SIMULTANEOUS MAIZE BREEDING FOR HIGH PROTEIN QUALITY AND STRESS TOLERANCE

Denić Miloje¹, Ignjatović-Micić Dragana¹, Stanković Goran¹, Marković Ksenija¹, Žilić Slađana¹, Lazić-Jančić Vesna¹, Chauque Pedro², Fato Pedro², Mariote David², and Haag Wayne³

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²Institute for agricultural research Mozambika, Maputo, Mozambik

³SG 2000, Maputo, Mozambique

Due to the low biological value of proteins of common maize, it was initiated breeding for high protein quality maize (QPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background. In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South Africa, then West, Central and Southern Africa and gene bank of Maize Research Institute “Zemun Polje” were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.177

BROADENING OF PERENNIAL RYEGRASS (Lolium perenne L.) AND COCKSFOOT (Dactylis glomerata L.) AUTOCHTHONOUS GENOTYPES COLLECTION

Sokolović Dejan¹, Babić Snežana¹, Tomić Zorica², Radović Jasmina¹, Lugić Zoran¹ and Cvetković Mirjana¹

¹Institute for forage crops, Krusevac

²Institute for Animal Husbandry, Belgrade

Perennial ryegrass and cocksfoot are most important fodder grasses. In Serbia, L. perenne is dominantly present in association Lolio-Cynosuretum cristati, especially on places treated upon intensively. D. glomerata is species of plain grasslands and in hilly regions it is mostly present on edges of mezoxerofile woods. In order to preserve genetic resources, but also to collect initial material for creation of well adopted and acclimatized forage cultivars, a collection of 10 L. perenne and 12 D. glomerata wild accessions was formed in the Institute for Forage Crops from 1989 to 1992 and sent to Serbian PGB. During previous period in domestic breeding projects, partly using material from National collection, three cultivars of these species were created. Further collection expanding is permanent objective of breeders in Serbia in order to improve genetic basis for breeding. In previous period several projects of gathering and evaluation of autochthonous genotype samples were carried out resulting with 51 cocksfoot and 5 perennial ryegrass new accessions. Those accessions are already incorporated in different breeding processes for cultivars with special fodder characteristics.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.178

GENETIC RESOURCES OF APPLE (Malus sp.) IN SOUTH SERBIA REGION

Mratinić Evica, Fotirić Milica

Faculty of Agriculture, Belgrade

Results of garnering, collecting and exact investigation in situ of 18 autochthonous apple cultivars from south Serbia region (Kosovska Mitrovica, Vranje and Preševo), are shown in this study. Important biologic properties such as flowering time (beginning, full bloom and abundance), ripening time, fruit size and fruit chemical characteristics (soluble solid, total sugar, acid and mineral matter content) and yield (kg/tree) were examined during period 2000-2002. Differences between apple cultivars studied in this experiment were determined by analysis of variance, while its clustering into similarity groups was done using the method of UPGMA. Studied apple cultivars had flowering span of 23 days (from 20^thApril till 5^thMay). According to the ripening time all cultivars were in the `winter` group. Cultivars with medium to large fruits, with high soluble solid and sugar content but low acid and mineral matter content were dominating. Also, majority of the apple cultivars had medium yields. In all examined cultivars alternative in bearing was noticed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.179

GENETIC VARIATION OF EUROPEAN SILVER FIR (Abies alba) IN SOUTHEASTERN EUROPE

Longauer Roman¹, Lavadinović Vera², Gömöry Dušan Paule Ladislav³, Borlea Gheorghe⁴, Ballian Dalibor⁵, Brus Robert⁶, Zhelev Petar⁷ and Andonoski Aleksandar⁸

¹Forest Research Institute, Zvolen, Slovakia;² Institute of Forestry, Belgrade, Serbia;³Technical University, Faculty of Forestry, Zvolen, Slovakia;⁴ROMSILVA - Forest Directorate Timisoara. Timisoara, Romania;⁵University of Sarajevo, Sarajevo, Bosnia and Herzegovina;⁶University of Ljubljana, Biotechnical Faculty, Ljubljana, Slovenia;⁷ University of Forestry, Sofia, Bulgaria;⁸ University St. Kiril and Metodij, Faculty of Forestry, Skopje, Macedonia

Genetic variation of 57 Southeast-European populations of Abies alba was studied by means of maternally inherited mitochondrial nad5-4 gene marker and isozyme gene markers in 18 nuclear loci.Geographic distribution of mtDNA haplotypes revealed 2 zones where different Postglacial re-colonization routes have met. The zone in the Ukraininan Carpathians is narrow, while two circumparallel migration streams are present in Central Dinarids: The haplotype typical of Balkanic refugia predominates in the maritime, whereas the Alpine-Hercynic haplotype in the inland part of the mountains. Clinal variation in the allele frequencies of nuclear gene loci indicate an extensive gene flow between areas colonized from different refugia, however. Mean observed heterozygosity (H_o=0.140-0.182), expected heterozygosity H_e= 0.143–0.188) and gene diversity (υ = 0.147–0.235) of Southeast-European populations appeared to be obviously higher than in the Central-European ones (H_o= 0.101–0.122, H_e= 0.102–0.124, υ = 1.114–0.142). This finding supports the hypothesis attributing the long-term dieback of Abies alba in Central Europe to insufficient genetic diversity (and reduced adaptability) of its local populations.Genetic differentiation in the isozyme loci proved to be higher in Abies alba than in other wind-pollinated forest tree species. The among-population component of total gene diversity accounted for 8.7%, and Nei’s genetic distances ranged from 0,019 to 0,037 between its Southeast- and Central-European populations. According to the genetic structure, southeastern populations of Abies alba can be grouped as follows: (i) Bulgaria, & southeast Serbia and Macedonia, (iii) Central Dinarids (iv) Romanian Carpathians and (v) Slovenia. Several alleles seem to be area-specific also within these regions, however.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.182

GENOTYPIC VARIABILITY OF GRAIN NUMBER PER SPIKE IN WHEAT (Triticum aestivum L.)

Knežević Desimir¹, Kovačević Vlado², Đukić Nevena³, Jelić Miodrag¹ and Luković Kristina⁴

¹Faculty of Agriculture, University of Pristina, Zubin Potok, Kosovo and Metohija, Serbia

²University J. J. Strossmayer in Osijek, Faculty of Agriculture, Osijek, Croatia

³Faculty of Natural Science, University of Kragujevac, Kragujevac, Serbia

⁴Economic Society Center for Small Grains doo Kragujevac, Kragujevac, Serbia

The mode of inheritance gene effect and combining ability and combining ability for number of kernels per spike in four divergent cultivars (jugoslavija, žitnica, NS rana 2 and osiječanka) were established in diallel crosses (without reciprocals) . Cultivars and hybrids expresed significant differences for the grain number per spike. This investigation established that the mode of inheritance was different with the preponderance of of partial dominace. The best general combiner for number of kernels per spike was Jugoslavija.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.183

VARIABILITY OF Gli-A2 ALLELES OF 21 Triticum durum CULLTIVARS

Đukić Nevena¹, Knežević Desimir² and Stamenković-Radak Marina³

¹ Faculty of Natural Science, University of Kragujevac, Kragujevac, Serbia; ²Faculty of Agriculture, University of Pristina, Zubin Potok, Kosovo and Metohija, Serbia; ³Faculty of Biology, Universityof Belgrade

In this paper, the composition of gliadins controlled by Gli-A2 alleles has been investigated, as well as polymorphism of alleles at Gli-A2 locus in 21 cultivar of durum wheat. Gliadins were separated by the method of electrophoresis on polyacrylamide gel. Electrophoregrams obtained by polyacrylamide gel electrophoresis were used for estimation variability of gliadin components and identification of gliadin blocks.

In the investigation, 9 alleles were identified (b, c, d, e, f, g, j, k and n) at Gli-A2 locus. Alleles b, c, d and e are encoding blocks whose components are located in a zone. The first components of blocks determined by alleles n, f, g, j and k is identical and located in b zone, and the other components are located in a zone. Gliadin blocks differed according to number of components and their molecular mass. Variability of determined block components indicates that existing polymorphisms of gliadins alleles Gli-A2 loci. Frequency of identified 9 alleles at Gli-A2 locus was different and varied from 4.76 % to 23.8 %. The most frequent was Gli-A2b allele and the least frequency was found for Gli-A2j, Gli-A2d, Gli-A2f.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.184

POSSIBILITIES OF USING PHYSIOLOGICAL PARAMETERS IN WHEAT BREEDING ON GRAIN YIELD

Olivera Nikolić¹, Tomislav Živanović², Milovanović Milivoje¹

Center for Small Grains Kragujevac, Kragujevac, Serbia

² Faculty of Agriculture, Belgrade-Zemun

Contemporary wheat selecting and breeding, in aim to obtain satisfactory results in increasing productive potential of new genotypes, need application of new criterions and different scientific knowledge as base. This paper deals with possibilities of using physiological parameters of wheat plant nitrogen nutrition efficiency as criterions in breeding on its grain yield, according to their interrelationships. The investigation included 30 wheat cultivars and perspective lines. The positive and statistically high significant interrelationships between nitrogen content in the above – ground part of plant in the flowering, in grain and in straw, entire nitrogen content in the matured plant, nitrogen reutilization and post – anthesis accumulation as physiological parameters and grain yield were registered in investigated material. Most of listed parameters, important by grain yield aspect, can be studied easily and measured before wheat vegetative period ended. The investigated parameters can be recommended as criterions for selecting parents pairs and evaluating progeny in breeding wheat on grain yield, considering their determined interrelationships and their measurable.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.185

spike stability parameters in wheat grown on solonetz soil

Petrović Sofija, Dimitrijević Miodrag, Banjac Borislav, Vukosavljev Mirjana

University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Phenotypic variability of the number and grain weight per spike, as well as the spike length of wheat varieties NSR-5, Evropa 90 and Pobeda creation of the Institute of Field and Vegetable Crops in Novi Sad was studied. The trial was conducted at the village Kumane locality in Banat on solonetz soil. Control and two levels of amelioration (25t/ha and 50t/ha of phosphor gypsum) in three vegetation periods were followed. The variety Evropa 90 exhibited maximal values of all examined spike traits in all years of study. Significant value of the first PCA was denoted for the grain number and the grain weight per spike, using AMMI model.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.186

INDUCTION OF DOUBLED HAPLOIDS IN MACEDONIAN

WHEAT GENOTYPES (Triticum aestivum L.)

Jankuloski Ljupcho¹, Jankulovska Mirjana¹and Simeonovska Emilija²

¹Faculty of Agricultural Sciences and Food, Skopje, Macedonia

²Institute of Agriculture, Skopje, Macedonia

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous doubled haploids from selected eight Macedonian winter wheat (Triticum aestivum L.) genotypes, from which six varieties and two advanced lines. For embryods induction and development of green plant regenerants, modified P-4 induction medium and modified 190-2 regeneration medium were used, respectively. Statistically significant differences were observed among wheat genotypes for androgenous capacity, green plant regeneration and doubled haploid production. From the whole material, the embryods production was 49.29 per 100 isolated anthers in average, ranging from 0.50 (Treska) to 119.67 (SK 15/90). The variety Treska was without capacity for green plant regeneration, while Bistra has the highest average green plant regeneration ability (3.92). In average, induction frequency for doubled haploid production was 0.86. Two genotypes (Treska and SK 15/90), didn’t show any response for doubled haploid production. From the total number of green plant regenerants, 37.55% were doubled haploid and fertile plants.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.187

EFFECT OF AUXIN CONCENTRATION ON THE GROWTH OF WHEAT HAPLOID EMBRYOS

Prodanović Slaven¹, Girek Zdenka² and Matzk Fritz³

¹ Faculty of Agriculture, Zemun-Belgrade, Serbia

² Institutute for Vegetable Crops, Smed.Palanka , Serbia

³Institute of plant genetics and crop plant research, IPK-Gatersleben, Germany

Different factors affect the development of haploid embryos derived after crossing wheat with maize. The aim of this research was to analyze the effect of auxin concentration on the growth and development of wheat haploid embryos and to determine the optimal auxin concentration. Three different concentrations of auxin dicamba (0.01 ppm, 1 ppm and 100 ppm) were applied. Embryo rescue from wheat caryopses was performed in 6 different developmental stages (from 3^rd to 14^th day after fertilization). Rescue was followed by measurement of the embryo size (length and width in mm) and by estimation of the embryo development stage (using the scale 1 to 3) in each rescued embryo. According to the results, the embryo size was affected by auxin concentration. Embryos were longer and wider when higher concentration of auxin was applied and this fact was more obvious as embryos were older. However, embryo development was not significantly affected by auxin concentration; 14 days after fertilization all embryos were at the same development stage. Since the larger embryos could be easier rescued and transferred on the medium for their growth in vitro, dicamba concentration of 100 ppm was recommended for the practical work.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.188

THE EFFECT OF ANDROGENIC RESPONSE COMPONENTS ON REGENERATION WHEAT PLANTS IN VITRO

Vukosavljev Mirjana¹, Kondić-Špika Ankica², Petrović Sofija³, Dimitrijević Miodrag³, Mladenov Velimir³ and Banjac Borislav¹

[1] Schoolar of Ministry of science and technology, Belgrade,Serbia

2 Institute of Field and Vegetable Crops, Novi Sad, Serbia

³ University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Specialized plant tissue culture methods have enabled the production of completely homozygous lines from gametic cells in a shortened time frame compared to conventional plant breeding. Plants derived from gametic cells represent a homozigous array each having a different genetic contribution from the parents. Wheat doubled haploid production is widely used not only for plant breeding acceleration but also in basic research, such as genomic mapping, haploid transformation and artificial seed production. The effect of two nutrient media, Potato-2 and N₆ on androgenetic induction on wheat anther culture was studied in vitro. Variation, interrelationships and path analysis for 4 components of androgenic response: frequency of responsive anthers, callus induction, green plant regeneration and albino plant regeneration of wheat were studied in two different environments using anther from 6 F₁ wheat lines. Correlation coefficient analyses showed positive correlation among anther culture traits. Desirable high significant influence on regeneration wheat plants in path coefficient analysis was found for number of green and albino plants in both media.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.189

WHEAT (Triticum aestivum L.) BORON TOLERANCE IN FIELD CONDITIONS

Brdar-Jokanović Milka¹, Kobiljski Borislav², Kraljević-Balalić Marija³, and Maksimović Ivana^2,3

¹ Institutute for Vegetable Crops, Smed.Palanka , Serbia

² Institute of Field and Vegetable Crops, Novi Sad, Serbia

³ University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

High concentration of soil boron may cause significant yield losses in wheat. The purpose of this study was to assess the impact of excess boron on yield and yield components in 59 wheat genotypes. Two-year field trial included control and three boron treatments. Selection criterion for boron tolerance was yield reduction on treatments with respect to control. Significant differences occurred among studied wheat genotypes for all analyzed traits. Cultivar Stephens was characterized by yield reduction of 20.1%, whereas yield of cultivar Evropa 90 was 9.7% higher on boron treatments with respect to control. Out of three yield components, number of grains/spike was the most affected by excess boron, with an average reduction of 8.6%. Average grain weight reduction was 2.9%. Number of spikes/m² was 7.0% larger on boron treatments than on control. In conditions of optimal boron supply yield correlated positively with all three yield components. Yield reduction on boron treatments with respect to control correlated only with number of spikes/m² reduction.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.190

IN VITRO EVALUATION OF BORON TOLERANCE IN WHEAT GENOTYPES

Kondić-Špika Ankica, Kobiljski Borislav, Marjanović Milica, and Hristov Nikola

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Boron tolerance of 14 wheat (Triticum aestivum L.) varieties was determined using the in vitro mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 and 30 mM. The control medium contained no excess boric acid. After one month of cultivation callus fresh weight was measured and reductions of fresh callus weight (RFCW) at different boron concentrations, in relation to the control, were calculated. Presence of excess boron in the nutrient medium caused an inhibition of callus growth in all of the genotypes. However, the genotypes differed significantly according their reaction to different boron concentrations. At the 15 mM boic acid concentration, cvs. Nevesinjka, Renesansa, Sonata, Simfonija, Rapsodija, and Pesma had RFCWs below 50%, while cvs. Pobeda, Venera, Balada, Ljiljana, Kantata, Cipovka, Vila, and Balerina had RFCWs above 50%. Among the genotypes, cv. Simfonija had the lowest RFCW (13.5%) and it was considered as the most tolerant genotype. The most sensitive genotype to excess boron was cv. Balada, with RFCW value of 87.1% at the concentration of 15 mM boric acid.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

Pp.191

EFFECT OF N MINERAL FERTILIZATION ON THE ALVEOGRAPHIC PARAMETERS OF WINTER WHEAT

Đurić Veselinka, Hristov Nikola, Kondić-Špika Ankica, Aćin Vladimir, Racić Marija

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Investigations on the baking quality of winter wheat, which is the most important bread cereal in Serbia, have been in the focus of attention for a long time. It is useful to study the theoretical and practical aspects of European quality testing systems, because different European methods are generally mutually accepted in the EU. Many recognised testing methods have been developed in Serbia over the last thirty years. In the present experiments studies were made on the effect of the year, variety and mineral fertilization and their interactions on alveographic parameters. in the years examined, the main factor which determined the alveographic values was found to be the variety. Fertilization had a significant effect on the examined parameters, but in most cases no regular trends were observed. The year only modified wheat quality in interactions.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.192

VARIATION OF N, P AND K CONTENTS IN GRAIN AND STRAW OF WINTER WHEAT GENOTYPES IN DEPENDENCE OF SYSTEM OF FERTILIZATION

Jelić Miodrag¹., Đalović, I.²., Knežević Desimir¹., Paunović Aleksandar³

¹Faculty of Agriculture, Zubin Potok, ;²Faculty of Agriculture, Novi Sad;

³Faculty of Agronomy, Čačak, Serbia

Different winter wheat genotypes were investigated in long-term field experiment with fertilization carried out in Center for Small Grains in Kragujevac, during 2000-20003. Wheat samples of grain and straw for analysis used in stage of full ripening. The investigated winter wheat had different concentration of the most important mineral elements: N, P and K. In average the highest grain N concentration (2.15 %) had KG-100 and the lowest (1.69 %) cultivar Matica, while in vegetative part, the highest concentration of nitrogen had Toplica (0.63%). The content of P in grain and straw of analyzed genotypes and its variation was significantly lower in relation to nitrogen contents. The lowest contents of P in grain (0.21%) and straw (0.051%) had cultivar KG-56, while the highest average content of P had cultivars Takovcanka and Studenica (0.31%). Concentration of K in straw was the significant higher than its concentration in grain of wheat cultivar. The highest contents of K (1.29%), had KG-100 while the lowest contents had Toplica (0.72%).The wheat genotypes reaction to application of mineral fertilizer was very different. The examined wheat genotypes realized the highest N concentration in grain and straw at NK variant of fertilization. Concentration of P in grain and straw as well K in grain was the highest on unfertilized plot (control), while concentration of K in straw was the highest on NK variant of fertilization.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.193

EFFECT OF FERTILIZATION ON OIL CONTENT IN WHEAT GRAIN

Hristov Nikola, Mladenov Novica, Đurić Veselinka, Kondić-Špika Ankica, Marjanović-Jeromela Ana and Lečić Nada

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Oil content in grain is a wheat quality indicator of importance in the food processing, cosmetics and pharmaceutical industries. Quality indicators may be significantly improved by applying appropriate cultural practices, especially fertilization. The objective of this study has been to assess the effect of fertilization on oil content variability in the grain of different wheat genotypes. A two-year study has included 24 phenotypically divergent genotypes. Analyses were conducted on the outer coat of wheat grain (the bran) separated during milling with a laboratory mill MLU 202. Oil content was determined by the conventional method of Rushkovsky. The analysis of variance showed that significant differences existed both among the genotypes and the applied doses of mineral fertilizers. Lowest average oil contents were found in the genotypes Skopljanka and Pobeda (3.25%), the highest in the genotype Dragana (4.88%). Oil content ranged from 3.10% to 4.98% in the non-fertilized variant and from 3.26% to 5.09% in the fertilized variant. The average oil contents in the respective variants were 3.90% and 4.08%, indicating that the tested cultural practice affected positively the analyzed characteristic.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.194

THE GRAIN YIELD OF BREAD WHEAT GENOTYPES ON THREE LEVELS OF NITROGEN NUTRITION

Gorjanović Biljana and Kraljević-Balalić Marija

University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

The constant increase of use of nitrogen fertilizers leads to numerous ecological and health problems. One of the ways to solve these problems is creating genotypes that will give the appropriate yield with limited application of mineral fertilizers. The goal of this paper is to investigate variability of grain yield, of twelve bread wheat genotypes, on three nitrogen level, and to classify genotypes according their phenotypic similarity for examined trait. ANOVA showed that this trait was mostly under influence of the genotype, year × genotype interaction, year of investigation, and in the smallest amount of the nitrogen rate. The grain yield was highest in 2006, and a smallest in 2007. On all three nitrogen levels, the highest grain yield was found in the variety Malyska. The lowest grain yield in control was found in the variety Nevesinjka, while in the N₇₅ and N₁₀₀ rates it was found in the variety Tamaro. By analyzing dendogram, it can be concluded that the genotypes Malyska i Sonata were singled out on all three nitrogen levels as the genotipes with the highest values, while a variety Tamaro and Ilona were singled out as a genotipes with the smolest value.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.195

GENETIC DETERMINATION OF TECHNOLOGICAL QUALITY OF WHEAT

(Triticum aestivum L.)

Knežević Desimir¹, Zečević Veselinka², Đukić Nevena³ and Konstantinov Kosana⁴

¹Faculty of Agriculture, University of Pristina, Zubin Potok, Serbia

²Faculty of Biopharming Backa Topola, University Megatrend Belgrade

³Faculty of Natural Science, University of Kragujevac, Kragujevac, Serbia

⁴Maize Research Institute „Zemun Polje“ Zemun, Serbia

Quality represents complex genetically determined traits. The numerous parameters are included in quality determination. In this work were presented analysis of sedimentation volume and loaf volume in 20 genetically different wheat cultivars. The gliadin composition were analyzed by electrophoresis method. The relationships between Gli-1, encoding gliadin proteins and quality components were analyzed. The high sedimentation protein volume and high loaf volume values were established in cultivars (KG.56, Rodna etc.) which carried Gli-B1b, Gli-D1b, Glu-D1d. Also, positive correlation between Gli-D2 and sedimentation volume as well as between Gli-B1l and loaf volume were established (Jugoslavija, Srbijanka, Balkan etc.). The identified alleles at the Gli-1 loci can use as markers of quality in wheat breeding. However, the analyzed components of technological quality are genetically determined, but not only by Gli-1 than by other factors (Gli-2, Gli-3, Glu-1as well ratio of gliadin/glutenin amount etc) which is necessary more study.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.196

VARIABILITY OF TECHNOLOGICAL QUALITY OF WINTER WHEAT

Zečević Veselinka¹, Knežević Desimir², Bošković Jelena¹, Mićanović Danica³and Luković Kristina⁴

¹ Faculty of biofarming, Bačka Topola

²Faculty of Agriculture, University of Pristina, Zubin Potok, Serbia

³Serbian Chamber of Commerce

⁴Center for Small Grains Kragujevac, Kragujevac, Serbia

Variability of technological quality components (sedimentation value, wet gluten content, water absorption and flour quality number) in six winter wneat cultivars (KG-56S, KG-100, Studenica, Takovčanka, Toplica and Lazarica) created in the Small Grains Research Centre of Kragujevac were investigated. Quality components were analysed during seven years (1998-2004) in the cultivars that were present in the macro-trial of Small Grains Research Centre. Standard deviation and coefficient of variation were calculated as indicators of variability. The results showed that the sedimentation value greater variability expressed by years (Cv=20.6%) than by cultivars. (Cv=17.0%). Sedimentation value had the largest variability, and the lowest water absorption (Cv=4.5%-years; Cv=5.3%-cultivars) of all investigated traits. Wet gluten content expressed high variability by years (Cv=10.9%) and cultivars (Cv=11.1%). Variability of quality number was also high by years (Cv=17.1%) and cultivars (Cv=12.3%). Cultivar Lazarica showed the lowest variability for sedimentation value, Studenica for water absorption, cultivar KG-56S for wet gluten content and Toplica cultivar for flour quality number.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.197

INHERITANCE OF STEM HEIGHT AND SECOND INTERNODE LENGTH

IN BARLEY HYBRIDS

Madić Milomirka¹, Paunović Aleksandar¹, Knežević Desimir2 and Zečević Veselinka³

¹Faculty of agronomy, Cacak

² Faculty of Agriculture, University of Pristina, Zubin Potok, Serbia

³ Faculty of biofarming, Bačka Topola

Stem height and the length of the second (basal) internode are highly important components of lodging resistance in barley. Stem height reducing genes in barley have different phenotypic effects as compared to Rht genes in wheat, resulting in highly brittle stems, disease susceptibility and low-quality malt of dwarf barley genotypes. A diallel crossing of five divergent barley genotypes (KG-1/90, NS-293, Jagodinac, KG-15 and KG-10/90) was conducted for the evaluation of the mode of inheritance of stem height and second internode length. The prevailing mode of inheritance of stem height in most combinations in the F₁ and F₂ generations ranged from partial dominance to superdominance. As for the second internode length, intermediary inheritance or dominance was the most common mode of inheritance of the trait in most combinations. Relative heterosis for stem height and second internode length ranged from 3.5% to 19.4% and from 3.1% to 17.5%, respectively. The analysis of genetic variance components suggested that the dominant gene effect predominated in the inheritance of stem height while the additive effect predominated in the inheritance of second internode length in F₁ generation as well as in F₂, which was also significantly controlled by dominant gene effects.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.198

RESULTS AND PERSPECTIVES OF WINTER TRITICALE BREEDING

IN KRAGUJEVAC

Milovanović Milivoje and Perišić Vladimir

Center for Small Grains Kragujevac, Kragujevac, Serbia

The paper presents the results of work on the breeding of winter 6x triticale in Small grains research Centre in Kragujevac, as well as prospects for the future contribution to the advancement of this species in Serbia on the basis of micro trials (5x5m²) - 2003-‘08. Up to now, the 8 new cvs. of w. triticale were recognized to the Center. KG cultivars of w. triticale are characterized by high productivity and many of desirable agronomic and physiologic traits. Latest perspective cvs. and lines (Trijumf, Žarko, KG.Tr.360/1-2, KG.Tr.102/6-2, KG.Tr.55/3, KG.Tr.110/3-2) significantly surpass the standards for grain yield (KG 20 and Favorite), and some of them have the results at the level of or above the well-known Polish cv. Presto. At that, the stem height of them restrained the average at the level of low and m.high cvs. (97-123 cm). Significant results have been achieved on the improvement of grain plumpness and protein content, too. In the future work on breeding, special attention will continue to be focused on the winter form of secondary hexaploid or substitution triticale, while work on the primary 6x, 8x and 4x forms will serve for the improvement of variability and researches.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.199

A VISUAL ESTIMATION OF THE PHENOTYPE AS AN INDICATOR OF RELATEDNESS OF MAIZE INBRED LINES

Babić Vojka, Babić Milosav, Filipović Milomir and Delić Nenad

Maize Research Institute, Zemun-Belgrade, Serbia

The prediction of hybrid performances is of a primary interest of all hybrid breeding programmes. Besides, the selection of the inbreds that will express high heterosis in crosses is of essential importance. There are many methods applied for the prediction of heterosis and they can be classified into the following groups: per se performances of parents; mitochondrial congruence; combining abilities; genetic divergence determined via geographic origin, morphological and agronomic traits or biochemical markers. Regardless of the advancements in biotechnology and marker techniques, the field trials still mostly consume time and funds and are unavoidable in hybrid breeding programmes. The aim of this study was to investigate whether a visual estimation of maize inbred genotypes, according to the UPOV descriptor, could be used to define homogenous groups by their relatedness. The results obtained by the application of statistical analyses showed a high concordance of the classification obtained by the Ward's method of cluster analysis, phenotypic estimations of maize inbred lines and available information on the origin. The discriminant analysis can be used as an ancillary tool in defining the number of groups, while the correspondence analysis can give a global survey of interrelationships of the observed material in form of continual variability.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.200

THE CHANGE OF GENETIC AND PHENOTYPIC VARIABILITY OF YIELD COMPONENTS AFTER RECURRENT SELECTION OF MAIZE

Deletić Nebojša, Stojković Slaviša and Gudžić Slaviša

University of Priština, Faculty of Agriculture Kosovska Mitrovica – Zubin Potok

This paper deals with 31 SSD lines from ZP-Syn-1 C₀ and 37 from ZP-Syn-1 C₃ maize populations. After line selection and seed multiplication in the first year of the study, the trials were set during two years in Kruševac and Zemun Polje, in RCB design with three replications. Additive and phenotypic variances of yield components were calculated, as well as the estimation of genetic variability narrowing by multivariation cluster analysis. The differences in additive and phenotypic variances between the cycles were significant for ear length only, and highly significant for grain row number per ear and for percent of root and stalk lodged plants. It means, a significant narrowing of additive and phenotypic variance occurred only for those three traits, and the other traits did not changed their variability by selection in a significant manner. However, according to cluster analysis, distances among genotypes and groups in the zero selection cycle were approximately double than in the third one, but group definition was better in the third selection cycle. It can suggest indirectly to a total variability narrowing after three cycles of recurrent selection.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.201

COMBINING ABILITIES FOR YIELD MAIZE OF LINES FROM DIFFERENT

SELECTION CIKLUS

Sečanski Mile¹, Živanović Tomislav², Šurlan-Momirović Gordana², Prodanović Slaven and Jovanović Snežana¹

¹ Maize Research Institute, Zemun Polje, Belgrade-Zemun

²Faculty of Agriculture, Belgrade-Zemun

The investigations included mean values, variability, heterosis and general and specific combining abilities after the method develop by Griffing, 1956a for six maize inbred lines and their hybrids related to the yield. Since the depression of the yield occurs in inbreds in inbreeding, this trait was greater in hybrids than inbreds, as expected. The highest average heterosis for the yield was determined in the hybrid B14 x B37 (209.8% ). The analysis of variance of combining abilities for the yield pointed out to highly significantly positive values of GCA and SCA for the observed trait in both years of investigation. Non-additive genes (dominance and epistasis) had the significant importance for yield heritability, indicating that the significant of values of GCA and SCA. The inbreds ZPL1 and ZPL2 were lines with the highest GCA effects, while hybrid combinations ZPL2 x B73,ZPL1 x ZPL2, ZPL1 x B90, ZPL1 x B91, ZPL1 x B99, ZPL2 x B90 and ZPL2 x B91 were populations with significant SCA effects in both years of investigation. They encompass both parents with high GCA effects or one parent with high GCA effects and other with low GCA effects. Furthermore, hybrid combination B 14 x B 37 had significant SCA effect and parents with low GCA effects. This is probably a result of additive gene effects (additive x additive) of the interaction between parents.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.202

THE MODE OF INHERITANCE AND HERITABILITY OF THE YIELD AND THE POPPING VOLUME IN POPPING MAIZE (Zеа mays L. everta) HYBRIDS

Pajić Zorica, Erić Uroš, Srdić Jelena, Drinić Mladenović Snežana and Filipović Milomir

Maize Research Institute, Zemun Polje, Belgrade, Serbia

The success in popping maize breeding depends on the efficiency of applied methods and the breeding material. Six self-pollinated popping maize inbreds of a different origin were used in the present study. The mode of inheritance and the heritability of the yield and the popping volume were observed by the method of the diallel analysis. Since GCA is an indicator of the additive genetic variance and SCA is an indicator of the non-additive genetic variance (dominance and epistasis), it can be concluded that the non‑additive genetic effect predominantly affected the popping maize grain yield level. It is also indicated by the GCA to SCA ratio, which amounted to 0.059 for the grain yield, that the non-additive component was significantly higher than the additive component. As the narrow‑ and the broad-sense heritability is estimated on the basis of values of variance components its determination was not possible due to the presence of epistasis. The GCA to SCA ratio (0.208) illustrates that the effect of non-additive components on the inheritance of popping volume is almost five fold-higher than the effect of the additive component. It was determined that the effect of the dominant genetic variance (N1 and N2) was higher than the effect of the additive component (D). A medium high narrow-sense heritability (h2ns=0.449) and a relatively high broad-sense heritability (h2bs=0.807) were expressed for the trait popping volume.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.203

AN EVALUATION OF THE TWO GROUPS OF HALF-SIB FAMILIES FOR GRAIN YIELD IN MAIZE (Zea mays L.)

Ivanović Mile, Stanisavljević Dušan, Stojaković Milisav, Treskić Sanja and Mitrović Bojan

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Unselected S₁ families of the narrow base maize population (P₁) are crossed as a common male parent with two unrelated inbred-tester lines (inbred A, and inbred B) to form two half-sib progeny groups (P₁A and P₁B, respectively). Forty progenies in each of the two half-sib (S₁*inbred-tester) populations were evoluated for several plant and yield component traits. The 40 half-sib progenis of each population groups were evaluated, along with four check cultivars, at four locations in 2008. A randomized incomplete block design with two replications was used in each enviroment. Averaged across locations, there were significant yield differences between populations (9.08t/ha vs 8.44t/ha, for P₁B and P₁A, respectively). The grain yield (t/ha) differences obtained between P₁B and P₁A corresponding progenies and mean progeny groups, indicated different specific combining abilities of inbred A and inbred B with the common S₁ lines.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.204

CORRELATION AND PATH ANALYSIS OF GRAIN YIELD AND MORPHOLOGICAL TRAITS IN TEST-CROSS POPULATIONS OF MAIZE

Srećkov Zorana¹, Nastasić Aleksandra², Ivanović Mile² and Boćanski Jan³

¹ Schoolar of Ministry of science and technology, Belgrade,Serbia

² Institute of Field and Vegetable Crops, Novi Sad, Serbia

³ University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

One of the goals of this paper was to determine relationship between grain yield, like the most important economic trait, and traits of the plant and ear that are influencing on the grain yield, in two test-cross populations, which are formed by crossing progenies of NSU₁population after 17 cycles of phenotypic recurrent selection and two testers, 568/II NS and B73. At 568/II NS testcrosses, grain yield had the highest value of genotypic coefficient of correlations with kernel row number. In second studied population the highest value of coefficient of correlations also was found between grain yield and kernel row number, but that relationship was negative. Path coefficient analysisprovides more information among variables than do correlationcoefficients. Because of that goal of this study also was founding the direct and indirect effects of morphological traits on grain yield. Desirable, high significant influence on grain yield, in path coefficient analysis, was found for ear height, in both studied populations. Plant height, in both testcross populations, and kernel row number and oil content, at B73 testcrosses, has high significant undesirable effect on grain yield.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.205

THE EFFECT OF YIELD COMPONENTS ON GRAIN YIELD IN DIFFERENT PROGENY TYPES OF AN F₃ MAIZE POPULATION

Stojković Slaviša, Deletić Nebojša, Biberdžić Milan and Aksić Miroljub

Faculty of Agriculture, University of Pristina, Zubin Potok, Serbia

This paper deals with the investigation of S₁ and HS progenies obtained from an F₃maize population. Those two progeny groups were created in 2004, and field trials were carried out in the period 2005-2006 at three locations near Aleksinac, Leskovac, and Kruševac, in RCB design. After genotypic correlation coefficients were calculated, they were used to calculate path coefficients and multiple determination coefficients. Analysis of path coefficients showed significant direct effects of the all studied traits in both S₁ and HS progeny types. In S₁progenies indirect effects were significant for the all paths, except for percent of stalk and root lodged plants through 1000 grain mass, 1000 grain mass through percent of stalk and root lodged plants, and for 1000 grain mass through number of grain rows. The strongest direct effect on grain yield in HS progenies was shown by percent of stalk and root lodged plants (0.68**), while number of grains per row gave the strongest negative direct effect on grain yield (-0.97**). Multiple determination coefficients were significant for the most of independent variables’ combinations in both progeny types. Numerous combinations composed of just few traits showed joint effects almost equal to the one showed by all seven independent variables.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.206

THE UTILISATION OF RELATED MAIZE INBREDS

IN THE HYBRID SEED PRODUCTION

Pavlov Jovan, Stanković Goran and Delić Nenad

Maize Research Institute, Zemun Polje, Belgrade-Zemun

The maize seed production is often characterised with a low yield of parental inbred lines and low seed quality. This disadvantage can be eliminated in problematic hybrids if related inbreds are used as female components. At the same time, properties of hybrids developed by the use of related inbred lines deviate from the original single cross hybrids.Four inbred lines of the BSSS background were crossed to the same male component. Simultaneously, inbred lines were inter-crossed, and then pollinated with the same male component. In such a way, beside four single cross hybrids another six hybrids in which related inbreds were used as a female component were developed. The two-replicate trail was set up in two locations in 2008. The yield of hybrids, inbred lines and their sister-line versions was observed. Obtained results show that related three‑way cross hybrids had yields at the level of the original single cross hybrids, and just in one case a single cross hybrid significantly overyielded a related three‑way cross hybrid. Yields of sister-line versions were higher by 27-58% than yields of the corresponding inbred lines. According to these results, the utilisation of related inbred lines as female components in the hybrid seed production is recommended for hybrids whose seed production is uncertain.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.207

THE PERFORMANCE OF MAIZE HYBRIDS WITH EXOTIC GERMPLASM UNDER CONDITIONS OF OUR COUNTRY

Filipović Milomir, Čamdžija Zoran and Lopandić Dragiša

Maize Research Institute, Zemun Polje, Belgrade-Zemun

White- and yellow-seeded exotic germlapsm, adapted to drought, was introduced in our mainly yellow-seeded continental germplasm within the selection programme for drought carried out at the Maize Research Institute, Zemun Polje, during the 1990s. Having good knowledge of heterotic pairs in the adapted and the exotic material, the suitable sources for the development of new yellow- and white-seeded inbred lines were derived very soon. Two white-seeded and three-yellow seeded maize hybrids were developed within this programme. The advantages of these hybrids developed within this selection programme were especially pronounced during dry years 2003 and 2007. In dry years, hybrids developed within this selection programme expressed their superiority in relation to yields and greater grain dry-down rates. This is well illustrated with the two-year results obtained by the Commission for the Variety Releasing in the process of testing and releasing hybrids ZP 555 and ZP 606. The superiority of these hybrids in relation to checks is especially pronounced in dry years such as 2007.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.208

YIELDS STABILITY OF COMMERCIAL MAIZE HYBRIDS

Babić Milosav¹, Babić Vojka¹, Prodanović Slaven² and Anđelković Violeta¹

¹ Maize Research Institute „Zemun Polje“, Zemun Polje

² Faculty of Agriculture, Belgrade, Serbia

Different interests of breeders and growers in regard to GxL (genotype x location) and GxY (genotype x year) interactions rises an important question: How broadly can a variety be adapted and at the same time have an optimum yield in a given location? Although scientists have been dealing with this problem for many decades, there is still a huge controversy over defining desirable adaptability and genotype stability. In accordance to certain definitions, some authors state that selection of stable genotypes will lead to selecting commercially quite unusable genotypes.Studying the literature considering these issues it was observed that there are many inconsistencies in regard to the concept of stability and its importance for the breeding aim. A complex phenomenon such as interaction is difficult to be explained by a universal parameter as it used to be case with many previous concepts of stability. In recent times, the increasing number of researchers have used hybrid statistical models in order to observe the interaction, and thereby stability. These models include multivariate statistical analyses, such as the additive main effect and multiplicative interaction (AMMI) model, the sites regression analysis (SREG) model or the shifted multiplicative model (SHMM). Fifteen commercial maize hybrids were used in the present study with the aim to observe the agreement of results of yield stabilities gained according to the AMMI model and Eberhart and Russell's model.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.209

STABILITY OF YIELD AND YIELD COMPONENTS IN

MAIZE HYBRIDS

Cvarkovic Radomir¹, Branković Gordana¹, Calic Irena¹, Delić Nenad², Živanović Tomislav¹ and Šurlan-Momirović Gordana¹

¹Faculty of Agriculture, Department of Genetics, Plant Breeding and Seed Production, Belgrade, Serbia

²Maize Research Institute „Zemun Polje“, Zemun Polje

Two-years yield and 1000-grains mass data of 24 maize hybrids of FAO maturity groups 400, 500, 600, 700 were analyzed. Investigations were performed at the two environments in two years. Nonparametric methods of the Hildebrand, Kubinger and the van der Laan–de Kroon showed genotype–environment interaction for both investigated features. Maize hybrids stability were estimated with: 1. the mean of the absolute rank differences over environments 2. the common variance of the ranks 3.and 4. the sum of the absolute deviations and sum of squares of rank relative to the mean of ranks. On the basis of the stability parametar values, the most stable and the most unstable hybrids were estimated for each FAO maturity group, for both investigated features. Correlation coefficients between both investigated features and stability parameters and for all pairs of stability parameters were computed. In spite of the positive correlations estimated between all four stability parameters, we can make groups: 1. the mean of the absolute rank differences over environments and the common variance of the ranks 2. the sum of the absolute deviations and sum of squares of rank relative to the mean of ranks.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.210

STABILITY PARAMETERS OF GRAIN YIELD IN MAIZE

HYBRIDS (Zea mays L.)

Đurović Dragan, Madić Milomirka and Stevović Vladeta

Faculty of agronomy Cacak

Stability parameters of grain yield were evaluated in 11 maize hybrids of FAO 300-700 maturity groups (ZPSC 330M, ZPTC 404, ZPSC 42A, ZPSC 480, ZPSC 539, ZPSC 599, ZPSC 580, ZPSC 677, ZPSC 633, ZPSC 704 and ZPSC 753) at three different locations in Central Serbia over a two-year period. The hybrids were tested in two separate trials including 50,000 and 65,000 plants/ha. The stability parameters were estimated using the Eberhart and Russell regression model (1966).There were no significant differences (except in the ZPTC 404 hybrid) between the values of the regression coefficient (b_i) for grain yield and the mean value. The ZPSC 599 hybrid of the group of hybrids with a medium growing season gave high yields and less favourable values of stability parameters at most locations and over most years as compared to the long-season hybrids. The late maturity hybrids (FAO 600 and 700) as compared to the early maturity ones generally exhibited unfavourable values of stability parameters, i.e. a specific response and better adaptation to more favourable environmental conditions, and produced higher average yields. The yield of these hybrids could not have been jeopardized by the yield of the early maturity hybrids.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.211

The EFFECTS of cytoplasmic male sterility and xenia on the chemical composition of maize grain

Vančetović Jelena¹, Jankuloski Ljupcho², Božinović Sofija¹ and Dodig Dejan¹

¹Maize Research Institute, Zemun Polje, Belgrade-Zemun, Serbia

²Faculty of Agricultural Science and Food, Skopje, Macedonia

Sterile hybrids often outyield their fertile counterparts, especially if pollinated by a genetically unrelated pollinator. The combined effect of cms and xenia is referred to as the Plus-hybrid effect. The objective of this study was to determine the individual, as well as, combining effect of cms and xenia on the maize grain chemical composition. The percent of oil, protein and starch in the grain was also observed. Two sterile hybrids, their fertile counterparts and five fertile pollinator-hybrids, were selected for the studies. The three-replicate trial set up according to the split-plot experimental design was performed at Zemun Polje in 2008. The obtained results show that the effects of cms on the oil percent was not significant in the studied hybrid ZP 341, while it increased at the significance level of P = 0.1 in the second observed hybrid ZP 360. The effect of this factor on the protein and starch percent was also significant (P = 0.01) in some hybrid combinations. Xenia effects on all three chemical parameters were significant (P = 0.01) in some hybrid combinations. The gained results indicate that the identification of a good combination of two hybrids, in which one would be a sterile female component, and the other a pollinator, would end up not only in the increased yield, but also in the improved maize grain quality.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.212

ANTIOXIDANT ACTIVITY IN SEEDS OF MAIZE GENOTYPES WITH DIFFERENT PERCENTAGE OF EXOTIC GERMPLASM

Kravić Natalija, Anđelković Violeta, Hadži-Tašković Šukalović Vesna and Vuletić Mirjana

Maize Research Institute Zemun Polje, Belgrade, Serbia

In order to broaden the genetic base of maize (Zea mays L.) germplasm, it is necessary to integrate exotic materials into adapted breeding materials. The aim of the study was to compare antioxidative systems of two adapted maize inbred lines (A and B) with exotic germplasm, Drought Tolerant Population (DTP), and their backcrosses with DTP (A1, A2 and B1, B2). The content of low-molecular weight antioxidants, proline and phenolics, as well as antioxidant capacity, detected as free radical scavenging activities against DPPH radical, were measured in maize seeds. Proline content in both, embryo and endosperm was higher in backcrosses than in inbred lines and DTP, and increased by getting higher percentage of exotic germplasm. Contrary, phenolic content and DPPH radical scavenging activity of seeds, which were higher in adapted inbred lines than in DTP, were slightly decreased in their backcrosses with DTP.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.213

COMPARISON OF RAPD AND SSR MARKERS FOR GENETIC DIVERSITY STUDIES IN MAIZE LOCAL POPULATIONS

Ristić Danijela, Ignjatović-Micić Dragana, Drinić Mladenović Snežana, Anđelković Violeta and Nikolić Ana

Maize Research Institute Zemun Polje, Belgrade, Serbia

Modern crop production is based on cultivating only some varieties and as a consequence the great part of genetic diversity has disappeared. With the reserve of ancestral genes, maize (Zea mays L.) local populations are a valuable source of genetic diversity and variability. The gene bank in Maize Research Institute “Zemun Polje” maintains the collection of 2178 local populations of maize, characterized and classified primarily using morphological markers. The main objective of our study was to investigate the applicability of SSR and RAPD markers, as well as DNA-pooling strategy (bulked samples), in genetic diversity determination. The genetic diversity within and among two local populations from Maize Research Institute (MRI) gene bank was estimated using five SSR primer pairs and five RAPD primers. Marker profiles for each primer were scored as presence/absence of individual bends within and between samples. Genetic distance between the analysed populations was calculated by Nei and Li (1979), while unweighted pair-group method analysis (UPGMA) was applied for cluster analysis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.214

SSR POLYMORPHISM ASSAY FOR MODIFIER LOCI IN NORMAL AND opaque2 Maize Inbred Lines

Ignjatović-Micić Dragana, Marković Ksenija, Drinić Mladenović Snežana

and Lazić-Jančić Vesna

Maize Research Institute Zemun Polje, Belgrade, Serbia

Nutritional value of maize is poor due to deficiency of two essential amino acids – tryptophan and lysine. Opaque2 (o2) mutations can nearly double the lysine and tryptophan content compared with the normal type. Incorporation of opaque2 into high yielding commercial cultivars failed, because of its numerous agronomic and processing problems. These drawbacks can be corrected with incorporation of modifier genes for endosperm hardness and amino acid content along with opaque2 into target genotypes, i.e. by developing quality protein maize (QPM). Maize Research Institute Gene bank maintains a collection of opaque2 inbred lines developed in 1970-ies. This material could be a valuable breeding source for development of QPM. In order to assay these lines for specific allele presence of the modifier genes in the opaque2 lines they were subjected to SSR analysis with endosperm hardness and amino acid modifier markers. No obvious correlation between modifier alleles and targeted traits content was established. This could be explained by the mechanism of gene regulation affected by o2 gene or inadequate distance between markers and genes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.215

HEREDITY IMPACTS ON PHOSPHORUS AND POTASSIUM CONCENTRATIONS IN MAIZE

Kovačević Vlado¹ and Šimić Domagoj²

¹University J. J. Strossmayer, Faculty of Agriculture, Osijek, Croatia

²Agricultural Institute, Osijek, Croatia

Twenty maize (Zeal mays L.) hybrids were grown under field conditions on acid (pH KCl = 4.77) hydromorphic soil (moderate supplies of available P and according AL-method: 7.91 P₂O₅and 9.17 K₂O/100 g) during the 2000 and 2001 growing seasons (4 replicates, plant density 71429 plants/ha, basic plot 7.0 m² = one 10-m row). Considerable differences of P and K status in the ear-leaf at silking stage were found among the maize hybrids. For example, they were from 0.269 % to 0.465% P, as well from 1.56% to 2.45% K. The lower P concentrations were found in three maize hybrids (OsSK378, OsSK298 and OsSK382: mean 0.276% P), while in four hybrids they were considerably higher (Alpos, Bc278, OsK2-191 and Clarica: mean 0.382% P). In two maize hybrids were found K concentrations lower than 1.60% K (OsSK333 and Os298: mean 1.57% K), while in five hybrids they were above 2.00% K (Os395, RK1456, Podravec 36, Os332 and Alpos: mean 2.17% K). Agreement of the leaf composition among the hybrids (comparison the results for 2000 and 2001) was considerably higher for P (r = 0.816**) than for K (r = 0.493*). Yield agreement was also significant (r = 0.587**). In both years of testing, negative correlation between yields and P status was found (r = -0.529* and -0.739**, for 2000 and 2001, respectively), while relationship between K and yields was low (r = -0.246 and -0.170, respectively).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.216

Đorđević Radiša, Zečević Bogoljub, Damnjanović Jelena, Todorović Goran and Jevđović Radosav

GENOTOXICITY TESTING OF EFFECTS OF NATURAL AGENTS

1Institute of plant protection and enviroment, Belgrade

Fruit research institute, Čačak

HEREDITY IMPACTS ON PHOSPHORUS AND POTASSIUM CONCENTRATIONS IN MAIZE

¹Institute of plant protection and enviroment, Belgrade