GENETIKA, Vol. 17, No.3 (1985)
J.C. GUPTA, B.K. MUKHERJEE and K.N. AGARWAL
EFFECT OF POPULATION STRESS ON GENETIC COMPONENTS OF VARIATION IN SYNTHESIZED MAIZE (ZEA MAYS L.) POPULATIONS [Abstract]
Kosana KONSTANT1NOV and M. DENIĆ
RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. II. GENOMIC CLONES IN STUDIES OF MAIZE GENOME ORGANIZATION [Abstract]
L. KOJIĆ and I. ŠATARIĆ
DIALLEL ANALYSIS OF GRAIN MOISTURE CONTENT IN MAIZE (Zea mays L.) [Abstract]
A.K. JAIN and R.K.S. RATHORE
EVALUATION OF COMBINING ABILITY IN EXOTIC RESTORERS AND INDIAN CULTIVARS OF WHEAT (TRITICUM AESTIVUM L.) [Abstract]
M. BALAKRISHNA REDDY, M.V. REDDY and B.S. RANA
SEASONAL VARIATION IN GENE EFFECTS FOR YIELD COMPONENTS IN SESAME (SESAMUM INDICUM L.) [Abstract]
K.N. SINGH, U. SINGH SANTOSHI and H.G. SINGH
HETEROTIC PARAMETERS IN PEA (Pisum sativum L.) [Abstract]
Estera MRČALICA
THE INFLUENCE OF PARENTAL SEX AND AGE ON THE SEX RATIO OF THEIR PROGENIES IN DROSOPHILA MELANOGASTER [Abstract]
N. ŽIVKOVIĆ, O. MILOVANOVIĆ, D. PAVIĆ, Đ. PRIJOVIĆ and P.N. MARTINOVIĆ
NONMENDELIAN INHERITABLE PRECOCIOUS OPENING OF THE VAGINA IN RATS AFTER EXPOSURE TO X-RAYS [Abstract]
Branka KRAJINČANIĆ, M. KIČIĆ, T JOVANOVIĆ, D. NINKOVIĆ and Vesna KRAJINČANIĆ
DISTRIBUTION OF ABO, Rh (D) AND MN BLOOD GROUPS EN THREE LOCALITIES OF SERBIA [Abstract]
Ljiljana VUKSANOVIĆ, Jugoslava PEČEVSKI, Nada SAVKOVIĆ and Dušanka RADIVOJEVIĆ
EFFECT OF VINBLASTINE AND VINCRISTINE ON THE INDUCTION OF MEIOTIC RECIPROCAL TRANSLOCATIONS IN MALE MICE AND THEIR Ft OFFSPRING [Abstract]
R. JUREČIĆ and Dražena PAPEŠ
ENDOPOLYPLOID SOMATIC CELLS WITH MORPHOLOGY OF ENDOMITOSIS IN SPERMATOGENESIS OF LEPTINOTARSA DECEMLINEATA SAY (COLEOPTERA: CHRYSOMELIDAE) [Abstract]
Zorica ČKREBIĆ, Gordana TROJANOVIĆ i Zvezdana POPOVIĆ
GENE TRANSFER IN THE EUKARYOTIC CELL, A PREREQUISITE FOR SUCCESSFUL GENE THERAPY [Abstract]
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 219-228
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.174.015.3
Original scientific paper
EFFECT OF POPULATION STRESS ON GENETIC COMPONENTS OF VARIATION IN SYNTHESIZED MAIZE (ZEA MAYS L ) POPULATIONS
J.C. GUPTA1, B.K. MUKHERJEE and K.N. AGARWAL
Indian Agricultural Research Institute New Delhi-110012, India
Abstract
Gupta J.C., Mukherjee B.K. and Agarwal K.N. (1985): Effect of population stress on genetic components of variation in synthesized maize (Zea mays L) populations. - Genetika, Vol. 17, No 3, 219-228.
Six synthesized, genetically broad base populations namely, KS14C, JML305, J236, JML24, DMR1 and DMR5 were used for studying gene effects at three densities. The three densities used were: below normal density (29,000 plants per hectare), normal density (56,000 plants per hectare) and above normal density (109,333 plants per hectare). The magnitude of epistatic contribution had exceeded additive, dominance and inter-variety dominance iii the case of five characters (except for grain yield) at above normal density, three characters (ear diameter, number of kernel rows per ear and 100-kernel weight) at normal density and two characters (number of kernel rows per ear and 100-kernel weight) at below normal density out of the total six characters studied. However, at below normal density, dominance component exceeded all other components in the majority of the characters.
Quantitatively, additive genetic and inter-variety dominance contributions were least affected by densities in a majority of the characters. Epistasis showed a somewhat increasing trend with increase in density for grain yield, ear diameter and number of kernels per row. All the parents possessed a preponderance of dominance gene effects in comparison to the corresponding additive gene effects in all the densities for all the characters. Dominance gene effects were over estimated in below normal density and under estimated in above normal density for grain yield. Parents like J236, DMR5 and JML305 which possessed large additive as well as dominance effects, not only for yield but for yield components especially in above normal density, can be subjected to reciprocal recurrent selection procedures to achieve further gains in high population stress conditions.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 229-235
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.852.113
Original scientific paper
RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. II. GENOMIC CLONES IN STUDIES OF MAIZE GENOME ORGANIZATION
Kosana KONSTANT1NOV and M. DENIĆ
Maize Research Institute, 11081 Beograd-Zemun, Yugoslavia
Abstract
Konstantinov Kosana and Denić M. (1985): Genomic clones in studies of maize genome organization. - Genetika, Vol. 17, No.3, 229 -235.
For the evaluation of genetic polymorphism of breeding material, both unique and repetitive DNA inserts were used as labelled probes. The investigated inbred lines were different concerning type of kernel (flint and dent type) and in the time they needed to attain maturity. When repetitive sequence was used as the probe, both quantative and qualitative differences between inbreds were obtained. With the unique DNA insert as the probe, polymorphism the methylation site was demonstrated both between inbred lines per se and between parental lines and their hybrid combinations.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 237-244
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.118.2/.3
Original scientific paper
DIALLEL ANALYSIS OF GRAIN MOISTURE CONTENT IN MAIZE (Zea mays L.)
L. KOJIĆ and I. ŠATARIĆ
Maize Research Institute "Zemun Polje", 11080 Beograd-Zemun, Yugoslavia
Abstract
Kojić L. and Šatarić I. (1985): Diallel analysis of grain moisture content in maize (Zea mays L.) - Genetika, Vol.17, No.3, 237-244.
Five divergent inbred lines were diallelly crossed in order to evaluate the mode of inheritance and combining ability of grain moisture content in maize at harvest. The tested material showed significant differences in respect to this characteristic.
Varying with the hybrid, grain moisture content was inherited with partial dominance, dominantly, whereas in some hybrids heterosis was observed.
The ratio GCA/SCA showed the predominance of additive over nonadditive gene effect. The highest GCA value was recorded for line B73, medium positive but not significant for inbreds Mo 17 and L105, and negative for L 233 and especially for A632.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 245-251
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.118
Original scientific paper
EVALUATION OF COMBINING ABILITY IN EXOTIC RESTORERS AND INDIAN CULTIVARS OF WHEAT (TRITICUM AESTIVUM L.)
A.K. JAIN and R.K.S. RATHORE
Department of Botany, R.B.S. College, Agra, India
Abstract
Jain A.K. and Rathore R.K.S. (1986): Evaluation of combining ability in exotic restorers and Indian cultivars of wheat. - Genetika, Vol. 18, No. 1, 245-251.
General and specific combining ability variances and effects for plant height, ear length, no. of spikelets (spike, no. of grains) ear, no. of tillers (plant, yield) plant, 100 grain weight, no. of days to flowering, no. of days to maturity and seed set percentage was studied in F1 progeny of 12 parents (8 Indian cultivars and 4 exotic restorers) of wheat (Triticum aestivum L.). Varieties and restorers were crossed reciprocally. Combining ability studies showed that in set-I the analysis of variances due to specific combining ability was much larger for all the characters than the general combining ability components of variances suggesting that all the characters were mainly controlled by dominant and epistatic types of gene action for different characters.
The hybrids of set-I provided significant SCA values for a greater number of hybrids than set-II. Therefore, the yield and its contribution characters were increased more for the larger no. of hybrids of set-I. Estimation of GCA clearly indicated that HDI593 was the best combiner in both the sets among Indian wheat cultivars. On the other hand, restorer R2 (3401/47466) was judged best for most of the characters among exotic germplasm.
Estimates of SCA for yield components and grain yield showed that HD2009 x R1 combination with significant positive SCA values i: for a number of characters seemed to be the best combination among the hybrids of set-I. Crosses HD2204 x R2 and HP1102 x R3 were also desirable with positively significant SCA effects for yield and its contributing characters. On the other hand, cross combination R2 x HD1593 did best among the hybrids of set-II for a number of characters.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 253-257
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.041/.048
Original scientific paper
SEASONAL VARIATION IN GENE EFFECTS FOR YIELD COMPONENTS IN SESAME (SESAMUM INDICUM L.)
M. BALAKRISHNA REDDY1, M.V. REDDY2 and B.S. RANA3
Department of Genetics and Plant Breeding, Andhra Pradesh Agricultural University, Rajendranagar, Hyderabad 500030 (AP)
Abstract
Balakrishna Reddy M., Reddy M.V. and Rana B.S. (1986): Seasonal variation in gene effects for yield components in sesame (Sesamum indicum L). - Genetika, Vol. 17, No. 3, 253-257.
Gene effects were studied in four crosses of sesame (Sesamum indicum L.) in kharif and summer seasons. In Fubilejniji varietal crosses, plant height, number of branches, effective stem length and capsules per plant were mainly governed by additive gene effects during kharif season. The significance of gene effects was low in summer as compared to the kharif season indicating the inconsistency of gene effects on various characters. For plant height and number of branches, duplicate epistasis was consistent over seasons while for other characters both duplicate and complementary epistasis, were observed. The variety Fubilejniji produced significant additive effects and was therefore considered useful for a breeding programme.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 259-267
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.222.78
Original scientific paper
HETEROTIC PARAMETERS IN PEA (Pisum sativum L.)
K.N. SINGH1, U. SINGH SANTOSHI2 and H.G. SINGH3
Department of Plant Breeding and Genetics, C.S. Azad
University of Agriculture and Technology, Kanpur-208002, India
Abstract
Singh K.N., Singh Santoshi U. and Singh H.G. (1986): Heterotic parameters in pea Pisum sativum L.). - Genetika, Vol. 17, No. 3, 259-267.
Heterotic parameters were estimated according to Gardner and Eberhart (1966). The sum of squares due to entries, varieties and various heterotic parameters were significant for most of the characters studied. The mean squares due to the residual effects in F1, were higher than the residual effects in the F2 generation for almost all the characters. The situation was similar regarding the differences for F1s V. F2s as compared to average heterosis. Considering average parental values and- parental effects, five strains were found superior for grain yield and three strains for protein content, were found superior. The cross combination, "T163 x EC33866" having the most diverse parents, produced the highest economic heterosis (60.77%), inbreeding depression (53.13%) and specific heterotic effect (18.30%) for grain yield. The cross 'BR12 x P209' producing maximum economic heterosis (4156%) and inbreeding depression (24.85%), produced a significant specific heterotic effect (4.06) for protein content. The cross combinations '6113 x BHU397', 'Rachna x 6113' and 'Rachna x BR12', for grain yield, and 'BHU397 x EC33866' and 'P209 x EC33866' for protein content, producing good performance in F2 are expected to be a reliable indicator of transgression in later generations.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 269-278
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.182/.183.8
Original scientific paper
THE INFLUENCE OF PARENTAL SEX AND AGE ON THE SEX RATIO OF THEIR PROGENIES IN DROSOPHILA MELANOGASTER
Estera MRČALICA
Faculty of Medicine, University of Ni š, 18000 Niš, Yugoslavia
Abstract
Mrčalica Estera (1985): The influence of parental sex and age on the sex ratio of their progenies in Drosophila melanogaster. - Genetika, Vol. 17, No. 3, 269-278.
The influence of parental sex and age on the secondary sex ratio of experimental populations of fruit fly Drosophila melanogaster is analyzed in this paper.
For the purpose of examining parents sex influence a large number of females were crossed with one male and the sex ratio of the progeny of each female was separately established at the imago stage. The age and masculinity rate ratio was analyzed through three types of crosses: of young males with young and then with old females; of young and then old males with young females; of old males with old females. It was found that the genomes of both parents were of influence in determining the sex ratio with this species.
Individual age influences decreased in the masculinity rate, the influence of the male parent being more strongly expressed. An increase in the percentage of female individuals in the progeny of old males is most likely due to genetic changes resulting in the formation of a greater number of X-hromosome spermatozoa. It seems that spermatozoa containing X-chromosomes are somewhat more successful in fertilizing the ova of old females.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 279-286
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.13/.224.2
Original scientific paper
NONMENDELIAN INHERITABLE PRECOCIOUS OPENING OF THE VAGINA IN RATS AFTER EXPOSURE TO X-RAYS
N. ŽIVKOVIĆ, O. MILOVANOVIĆ, D. PAVIĆ, Đ. PRIJOVIĆ and P.N. MARTINOVIĆ
Laboratory of Molecular Biology and Endocrinology Boris "Kidrič" Institute of Nuclear Sciences
11 001 Beograd, Yugoslavia
Abstract
Živković R., Milovanović O., Pavić D., Prijović D. and Martinović P.N. (1986): Nonmendelian inheritable precocious opening of the vagina in rats after exposure to X-rays.- Genetika, Vol. 17, No. 3, 279-286.
A highly significant inheritable advancement in opening of the vagina in rats has been induced by application of X- rays to embryos and infant animals. The phenomenon of precocity was followed-up in the progeny of the irradiated rats.
When pregnant females were exposed to moderate doses (2.9 and 1.9 Gy) on day six of gestation, the first appearance of advanced vaginal opening was noted in the F2 generation, and the lowest time limit of the mean value for precocity was reached in the F19 and F20 generations.
In the infant rats locally irradiated with high doses of X- rays (8.6 or 9.6 Gy), opening of the vagina appeared as early as in the first filial generation. A striking dose effect was clearly demonstrated in these experiments.
Crosses between rats belonging to the irradiated lines and normal partners showed that the factor responsible for precocity can be transmitted to the filials with equal effectiveness by both the male and female parent.
In contrast to the experimental animals, not a single case of precocity was noted in the inbred and random- bred controls.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 287-291
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.174.015.3
Original scientific paper
DISTRIBUTION OF ABO, Rh (D) AND MN BLOOD GROUPS EN THREE LOCALITIES OF SERBIA
Branka KRAJINČANIĆ, M. KIČIĆ, T JOVANOVIĆ, D. NINKOVIĆ and Vesna KRAJINČANIĆ
Faculty of Defectology, Beograd University, Military Academy and Medicine, Beograd
and Health Centre - Zemun, 11000 Beograd, Yugoslavia
Abstract
Krajinčanić Branka, Kičić M., Jovanović T., Ninković D. and Krajinčanić Vesna (1986): Distribution of ABO, Rh (D) and MN blood groups in three localities of Serbia. - Genetika, Vol. 17, No. 3, 287-291.
An investigation on some somatic and genetic characteristics is being carried out in three localities of Serbia, which are geographically far from one another and different according to the -degree of urbanization and origin of the population. Thus, the blood groups of 756 children from the vilages of Laznica and Žagubica in Homolje (Eastern Serbia) 542 children from Čajetina - Zlatibor (Western Serbia) and 416 children from Velika Drenova (Western Morava) have been examined. Haemogenic characteristics such as the ABO system, Rh-factor and MN system, have a very significant place in population genetics and make an important contribution to the genetic characteristics of the population.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 293-298
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.224.46.044
Original scientific paper
EFFECT OF VINBLASTINE AND VINCRISTINE ON THE INDUCTION OF MEIOTIC RECIPROCAL TRANSLOCATIONS IN MALE MICE AND THEIR Ft OFFSPRING
Ljiljana VUKSANOVIĆ, Jugoslava PEČEVSKI, Nada SAVKOVIĆ and Dušanka RADIVOJEVIĆ
Institute of Nuclear Sciences "Boris Kidrič", Laboratory of Radiobiology, 11000 Belgrade, Yugoslavia
Abstract
Vuksanović Ljiljana, Pečevski-Jugoslava, Savković Nada and Radivojević Dušanka: Effect of vinblastine and vincristine on the induction of meiotic reciprocal translocations in male mice and their fi offspring. -.Genetika, Vol. 17, No. 3, 293-298.
The dose-dependent effect of Vinblastine (VLB) and Vincristine (VCR) on the fertility and the indiduction of chromosomal translocations in treated mice and their F1 male offspring were examined. Single i.p. treatment with VLB and VCR (doses: 0.5; 1.0 and 2.0 mg/kg body weight) reduced the litter size in all groups VCR reduced fertility (65%) in the group treated with 2.0 mg/kg as well as testis weight (38%) in the same group of directly treated mice. Both VLB and VCR induced chromosomal translocations in parental males. VLB after the dose of 2.0 mg/kg (1.94%) and VCR after the dose of 1.0 mg/kg (0.12%). Cytological analysis of fi males revealed no chromosomal translocations. There was a significant enhancement of sex univalent among all treated mice.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 299-304
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.224.234.2
Original scientific paper
ENDOPOLYPLOID SOMATIC CELLS WITH MORPHOLOGY OF ENDOMITOSIS IN SPERMATOGENESIS OF LEPTINOTARSA DECEMLINEATA SAY (COLEOPTERA: CHRYSOMELIDAE)
R. JUREČIĆ and Dražena PAPEŠ
Laboratory of Cytogenetics, Department of Biology, Faculty of Science, University of Zagreb, 41001 Zagreb, Yugoslavia
Abstract
Jurecic R. and PapeS Drazena (1986): Endopolyploid somatic cells with morphology of endomitosis in spermatogenesis of Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). - Genetika, Vol. 17, No. 3, 299-304.
The present study describes the occurrence of endomitosis in cystocytes and cells of the inner partial layer of the testis follicle in Colorado potato beetle males. Besides cells in interphase, cells in endoprophase and endometaphase were most often encountered. Their morphology and function in gametogenesis of beetles are briefly discussed.
Published in „GENETIKA“ Vol. 17, No.3 (1985), pp. 305-313
© 1985 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.116.14
Review paper
GENE TRANSFER IN THE EUKARYOTIC CELL, A PREREQUISITE FOR SUCCESSFUL GENE THERAPY
Zorica ČKREBIĆ, Gordana TROJANOVIĆ i Zvezdana POPOVIĆ
Department of Molecular Biology, Institute of Biology "S. Stanković", 11000 Beograd, Yugoslavia
Abstract
Čkrebić Zorica, Trojanović Gordana and Popović Zvezdana (1985): Gene transfer in the eukaryotic cell a prerequisite for successful gene therapy. - Genetika, Vol. 17, No. 3, 305-313.
The introduction of cloned genes into animal cells offers an opportunity to study the expression of heterologous genes in a transformed host and therefore provides a system in which the functional significance of various features of DNA sequence organization can be examined in vivo.
Here, we describe three different eukaryotic vectors (pSV, BPV and retroviral vector), constructed to efficiently introduce and express foreign DNA in eukaryotic cells.