GENETIKA, Vol

GENETIKA, Vol. 23, No.2 (1991)

S. ŠESEK

THE EFFECT OF ANTHER LENGTH AND MICROSPORE DEVELOPMENT STAGE ON ANDROGENOUS PROCESSES IN WHEAT ANTHER CULTURE [Abstract]

Snežana MEZEI, Sibila JELASKA and L. KOVAČEV

CALLUS INDUCTION AND CALLUS TISSUE PROLIFERATION IN SUGARBEET CULTURE [Abstract]

Marija KRALJEVIĆ-BALALIĆ, T. MIŠIĆ, M.R. SARIĆ and Vojislava MOMČILOVIĆ

INHERITANCE OF NITROGEN CONTENT IN WHEAT [Abstract]

V. MIHAILOVIĆ, Marija KRALJEVIĆ-BALALIĆ and I. PATAKI

INHERITANCE OF SEED YIELD IN SPRING FORAGE PEAS [Abstract]

Kosana KONSTANTINOV, Snezana MLADENOVIĆ and M. DENIĆ

RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. V. PLANT AND INDIGENOUS BACTERIAL STRAINS TRANSFORMATION BY THE GENE CONTROLLING KANAMYCIN RESISTANCE [Abstract]

Zorica PAJIĆ and M. BABIĆ

INTERRELATION OF POPPING VOLUME AND SOME AGRONOMIC CHARACTERISTICS IN POPCORN HYBRIDS [Abstract]

Jelena LEVIĆ and Viktorija PENČIĆ

GENETICS AND BREEDING FOR LEAF DISEASE RESISTANCE OF MAIZE [Abstract]

L. DOKMANOVIĆ, S. LABAN, V. IVANOVIĆ-DERETIĆ and D. ŠKORIĆ

A PERICENTRIC INVERSION OF CHROMOSOME 9 ASSOCIATED WITH MILD MENTAL RETARDATION, MAJOR AND MINOR ANOMALIES, PANCYTOPENIA, AND WILMS'TUMOR [Abstract]

Nevenka MARTINOVIĆ, Vukosava DIKLIĆ, A. KRUNIĆ and Zorana DJAKOVIĆ

MECHANISM OF INHERITANCE OF ANDROGENIC ALOPECIA IN WOMEN [Abstract]

Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 89-95

Serbia

UDC 575.854 :576.744.4

Original scientific paper

THE EFFECT OF ANTHER LENGTH AND MICROSPORE DEVELOPMENT STAGE ON ANDROGENOUS PROCESSES IN WHEAT ANTHER CULTURE

S. ŠESEK

Institute of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia

Abstract

Šesek, S. (1991): The effect of anther length and micraspore development stage on androgenous processes in wheat anther culture. - Genetika, Vol. 23, No. 2, 89-95.

The effects of anther length and stage of microsporogenesis on androgenous capacity and plant regeneration ability were studied in wheat (Triticum aestivum L.) anther culture. In our experiment, anthers 1.5-2.0 mm long contained predominantly microspores in early uninucleate stage, anthers 2.1-2.5 mm long had microspores in miduninucleate stage, and anthers 2.6-3.0 mm long contained microspores in late uninucleate stage of pollen development.

The analyses of androgenous capacity showed that the anthers of 2.1-2.5 mm in length, with microspores in the mid-uninucleate stage, gave the best results i.e. 30% of the isolated anthers were androgenous. The same anthers had the highest plant regeneration ability (7.6%).

Androgenous capacity and plant regeneration ability in 2.6-3.0 mm long anthers, which contained predominantly microspores in the late uninucleate stage, were high also, namely, 26.9%, and 7.1% respectively. This means that the anthers of 2.1-3.0 mm in length were suitable for isolation.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 97-104

Serbia

UDC 75.854 : 576.7

Original scientific paper

CALLUS INDUCTION AND CALLUS TISSUE PROLIFERATION IN SUGARBEET CULTURE

Snežana MEZEI¹, Sibila JELASKA² and L. KOVAČEV¹

¹ Institute of Field and Vegetable Crops, Faculty of Agriculture, 21000 Novi Sad

² Institute of Molecular Biology, Faculty of Natural Sciences, 41000 Zagreb, Yugoslavia

Abstract

Mezei S., S. Jelaska and L. Kovačev (1991): Callus induction and callus tissue proliferation in suqarbeet culture. - Genetika, Vol. 23, No. 2, 97-104.

Experiments were conducted on five genotypes which differed in genetic constitution. Initiation and proliferation of callus was studied on cotyledon, hypocotyl and leaf explants. The analysis seems to indicate that the tested explants give identical callus color and structure. Nutrient medium PGoB supplemented with 3% sucrose, 0.7% agar, 5.4 /μM NAA and 0.3 μ/M BA was found most favourable for callus growth. Leaf explants of the F₁ hybrids differed significantly in callus production.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 105-110

Serbia

UDC 275.172/12 : 577.21

Original scientific paper

INHERITANCE OF NITROGEN CONTENT IN WHEAT

¹Marija KRALJEVIĆ-BALALIĆ, ¹T. MIŠIĆ, ²M.R. SARIĆ and ¹Vojislava MOMČILOVIĆ

¹ Faculty of Agriculture and Faculty of Natural Sciences

² University of Novi Sad, 21000 Novi Sad, Yugoslavia

Abstract

Kraljević-Balalić, M., T. Mišić, M.R. Sarić and V. Momčilović (1991): Inheritance of nitrogen content in wheat. - Genetika, Vol. 23, No. 2,105-110.

In order to study the inheritance, gene effects and combining ability for N content in the vegetative part and grain of wheat, a diallel analysis of four wheat cultivars (Jugoslavija, Osječanka, Žitnica and NS Rana 2) was carried out and the F₁ was analysed. The combining ability analysis was made according to Griffing (1956). The cultivars and the F₁ hybrids differed significantly in N content in the vegetative part and grain. Highly significant general combining ability (GCA) and specific combining ability (SCA) effects were obtained for N content, indicating the presence of both additive and non-additive components of genetic variance.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 111-119

Serbia

UDC 575.111/041'048

Original scientific paper

INHERITANCE OF SEED YIELD IN SPRING FORAGE PEAS

V. MIHAILOVIĆ, Marija KRALJEVIĆ-BALALIĆ and I. PATAKI

Institute of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia

Abstract

Mihailović, V., M. Kraljević-Balalić and I. Pataki (1991): Inheritance of seed yield in spring forage peas. - Genetika, Vol. 23, No. 2, 111-119.

Grain yield per plant has been analysed in 4 x 4 diallels, in the F₁ and F₂ generation of spring forage peas. The mode of inheritance was evaluated on the basis of the mean values of the generations in relation to the parents' average. Heterosis, dominance of the higher yielding parent and intermediacy were displayed in the F₁ generation and intermediacy and partial dominance in the F₂ generation.

The additive component of genetic variance was higher than the dominant component value which showed that the additive gene action was more important than the dominance and epistasis.

The varieties Poneka and Tarra were the best combiners for yield. They should be used in the further hybridization program and recombined with some other varieties which possess other desirable characters.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 121-135

Serbia

UDC 575.3/7 : 15

Original scientific paper

RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. V. PLANT AND INDIGENOUS BACTERIAL STRAINS TRANSFORMATION BY THE GENE CONTROLLING KANAMYCIN RESISTANCE

Kosana KONSTANTINOV, Snezana MLADENOVIĆ and M. DENIĆ

Maize Research Institute "Zemun Polje", 11081 Beograd - Zemun, Yugoslavia ;v:-ri

Abstract

Konstantinov, K., S. Mladenović and M. Denić (1991): Recombinant DNA technology in maize breeding. V. Plant and indigenous bacterial strains transformation by the gene controlling kanamycin resistance. - Genetika, Vol. 23, No. 2, 121-135.

The gene producting NPT 11 fused to the 35S RNA promoter of cauliflower mosaic virus (CaMV) and introduced in to pRT100neo plasmid (obtained from Reinhard Topfer, MP1, Cologne) was injected into maize plants before micro- and macro-sporogenesis during the summer of 1986. Marker gene expression was tested in the plains surviving in kanamycin sulphate concentration toxic for untransformed genotypes. Green plant progeny was tested both for g⁶¹¹⁶ expression and gene presence by Southern analysis of genomic DNA from T₄ plants (transformed plant of the fourth generation). Both NPT II en7,yme activity and positive hybridization with a DNA fragment containing the coding sequence for the enzyme NPT 11 have been obtained in several plants.

Typical Mendelian 3:1 segregation was not obtained for any plant; a lower number than the theoretical 25% of recessive phenotypes appeared in all transformed plant progeny. This result and the rather uniform hybridization of plant DNA and the DNA of the introduced plasmid pRT100neo initiated an experiment to test whether, besides plant genome transformation endogenous/indigenous bacteria transformation is going on side by side. On the medium containing kanamycin and ampicillin bacterial colonies developed only from the tissue of the transformed plant. Two kanamycin resistant bacterial strains have been identified in plant tissue as Pseudomonas maltophylia and Acinetobacter calcoaceticus.

As Pseudomonas strains are effective colonizers of maize root and stem, experiments on the presence and isolation of bacteria living in different maize tissues have been done. On nutritive medium colonies developed from embryo, endosperm, immature anther, pollen grains and leaves. None of these bacterial colonies grew on kanamycin. When bacterial DNA isolated from transformed P. maltophylia and A. calcoaceticus was used as the labelled probe, quite clear homology with bacteria found in normal maize tisseu was observed. Experiments directed at genetic analysis of endogenous/ indigenous bacterial strains of maize are in progress.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 137-144

Serbia

UDC 575.222.7/164

Original scientific paper

INTERRELATION OF POPPING VOLUME AND SOME AGRONOMIC CHARACTERISTICS IN POPCORN HYBRIDS

Zorica PAJIĆ and M. BABIĆ

Maize Research Institute "Zemun Polje", 11000 Belgrade- Zemun, Yugoslavia

Abstract

Pajic, Z. and M. Babi6 (1991): Interrelation of popping volume and some agronomic characteristics in popcorn hybrides. - Genetika, Vol. 23, No. 2,137-144.

Expansion volume, as the ratio between kernel volume before and after popping, is considered the most important quality trait in popcorn (Zea mays L).

Studies presented in this paper include the interrelations of individual characteristics crucial for growing popcorn. These characteristics are grain yield, as an economically important factor, and popping volume as an indicator of quality of the popcorn kernel.

On the basis of experimental results, a negative correlation was found between grain yield and popping volume (-0.18). A strong correlation was established between the number of kernels per 10 g and the popping volume (0.91**). These results are in agreement with the results of a number of other authors. A greater number of kernels per 10 g indicates that small kernels contain a larger portion of hard endosperm and very little, or only traces, of soft endosperm.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 145-166

Serbia

UDC 575.164.631.52

Original scientific paper

GENETICS AND BREEDING FOR LEAF DISEASE RESISTANCE OF MAIZE

Jelena LEVIĆ and Viktorija PENČIĆ

Maize Research Institute 'Zemun Polje', 11081 Belgrade - Zemun, Yugoslavia

Abstract

LeviC J. and V. Pendifi (1991): Genetics and breeding for leaf disease resistance of maize. - Genetika, Vol. 23, No. 2, 145-166.

The present report is aimed at elucidating the phytopathological-genetic-breeding approach in developing resistant maize hybrids for control of major economically important maize leaf diseases. The solution of this problem requires a number of prerequisites. Particularly important is the knowledge of the following factors: harmfulness of different pathogenic species; pathogen biology and ecology; pathogen variability; methods of artificial inoculation and resistance evaluation; resistance of different maize genotypes; mode of resistance inheritance and methods of resistance incorporation, i. e. resistance improvement.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 167-171

Serbia

UDC 575.224.232.4

Original scientific paper

A PERICENTRIC INVERSION OF CHROMOSOME 9 ASSOCIATED WITH MILD MENTAL RETARDATION, MAJOR AND MINOR ANOMALIES, PANCYTOPENIA, AND WILMS'TUMOR

L. DOKMANOVIĆ, S. LABAN, V. IVANOVIĆ-DERETIĆ and D. ŠKORIĆ

University Children's Hospital, 11000 Belgrade, Yugoslavia

Abstract

Dokmanović, L, S. Laban, V. Ivanović-Deretić and D. Škorić (1991): A pericentric inversion of chromosome 9 associated with mild mental retardation, major and minor anomalies, pancytopenia, and Wilms' tumor. - Genetika, Vol. 23, No. 2,167-171.

Two children with pericentric inversion on chromosome 9 are reviewed. They presented with mental retardation of minor grade and numerous minor anomalies. One child suffered from pancytopenia, while the other had wilms' tumor.

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Published in „GENETIKA“ Vol. 23, No.2 (1991), pp. 173-180

Serbia

UDC 575.164

Original scientific paper

MECHANISM OF INHERITANCE OF ANDROGENIC ALOPECIA IN WOMEN

Nevenka MARTINOVIĆ, Vukosava DIKLIĆ, A. KRUNIĆ and Zorana DJAKOVIĆ

Clinic for Dermatovenerology of the University Clinical Center and

Institute of Biology and Human Genetics of the Belgrade Medical School 11000 Belgrade, Yugoslavia

Abstract

Martinović, N., V. Diklić, A. Krunic and Z. Djakovic (1991): Mechanism of inheritance of androgenic alopecia in women. - Genetika, Vol. 23, No. 2, 173-180.

Genealogic analysis of 100 families of study subjects indicates the autosomally dominant type of androgenic alopecia heredity, considering that they were registered in 81.8% of descendants in cases where both parents were affected (ratio between affected and healthy was 1:1) and that they were registered in descendants of both sexes, irrespective of whether both parents were affected or only the father or only the mother. In 20% of families the study subjects were the only affected members, and the occurrence of AA may be explained by a new mutation.

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