GENETIKA, Vol. 23, No.3 (1991)
F. STUMPF, Lidija HALDA and W. KLINGMULLER
CONSTRUCTION OF JF 12, A SMALL PLASMID CONTAINING NIF GENE CLUSTER FROM ENTEROBACTER AGGLOMERANS 333 [Abstract]
Snežana MLADENOVIĆ, Kosana KONSTANTINOV, M. DENIĆ and Bojana TADIĆ
RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. VI. FOREIGN GENE INFLUENCE ON MAIZE GENOME EXPRESSION AFTER PLANT TRANSFORMATION BY DIFFERENT TECHNOLOGIES [Abstract]
Vesna LAZIĆ-JANČIĆ, D. KOVAČEVIĆ and M. DENIĆ
THE EFFECT OF ALUMINIUM ON GENE EXPRESSION IN MAIZE [Abstract]
Z. TOMOVIĆ and Branka KOLEVSKA-PLETIKAPIĆ
PLANT REGENE-RATION FROM CALLUS TISSUE CULTURES OF POPLARS [Abstract]
Maria D. OCHANDO and C. DE JUANA
MORPHOLOGICAL GENETIC VARIABILITY IN FIVE NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER [Abstract]
L. KOJIĆ, I. HUSIĆ, R. STOJŠIN and G. DRINIĆ
DIALLEL ANALYSIS OF KERNEL MOISTURE CONTENT AFTER BLACK LAYER FORMATION IN MAIZE [Abstract]
J. ĐORĐEVIĆ and M. FILIPOVIĆ
GENE EFFECTS FOR EAR LENGTH IN B73 AND B84 CROSSES OF MAIZE (ZEA MAYS L.) [Abstract]
R. CEROVIĆ
CYTOGENETIC PROPERTIES OF SOUR CHERRY IN RELATION TO POLLEN [Abstract]
P. CINDRIĆ, Nada KORAĆ and Mira MEDIĆ
A NEW GRAPE VARIETY RESISTANT TO PLASMOPORA VITICOLA [Abstract]
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 181-190
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.111/041'048
Original scientific paper
CONSTRUCTION OF JF 12, A SMALL PLASMID CONTAINING NIF GENE CLUSTER FROM ENTEROBACTER AGGLOMERANS 333
F. STUMPF1, Lidija HALDA2 and W. KLINGMULLER1
1Lehrstuhl fur Genetik, Universitat Bayreuth, P.O. Box 101251, D-8580 Bayreuth, Germany
2Maize Research Institute "Zemun Polje", 11080 Belgrade-Zemun, Yugoslavia
Abstract
Stumpf, F., L. Halda and W. Klingmuller (1991): Construction of JF12, a small plasmid containing nif gene cluster from Enterobacter agglomerans 333. - Genetika, Vol. 23, No. 3, 181-190.
JF12, a small plasmid containing the nif gene cluster of Enterobacter agglomerans 333 arid part of pACYC177 plasmid has been constructed. Cosmids peaMS2-2 and peaMS2-16, originally the cosmid pHC79, which contain various nif genes of strain Enterobacter agglomerans 333, were included in the experiment. Small fragment of peaMS2-2 (e.g. nifH, D, K, Y, E, N, X, U, S, V, M, L, A and B genes) and an EcoRI fragment of pea MS2-16 (e.g. nif A, B, Q, F and J) were selected as a source for the nif genes. Plasmids pACYC177 and pUC18 were chosen as hosts for cloning the isolated nif fragments. Fusion of an Apal fragment of the pUC18 plasmid with the pACYC177 plasmid resulted in plasmid pJF12 sized 33kb. The ability of the construction to fix nitrogen in Escherichia coli HB101 and C600 as a host was confirmed.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 191-204
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.575.47.633.15
Original scientific paper
RECOMBINANT DNA TECHNOLOGY IN MAIZE BREEDING. VI. FOREIGN GENE INFLUENCE ON MAIZE GENOME EXPRESSION AFTER PLANT TRANSFORMATION BY DIFFERENT TECHNOLOGIES
Snežana MLADENOVIĆ, Kosana KONSTANTINOV, M. DENIĆ and Bojana TADIĆ
Maize Research Institute "Zemun Polje", 11080 Zemun - Belgrade, Yugoslavia
Abstract
Mladenović, S., K. Konstantinov, M. Denić and B. Tadić (1991): Recombinant DNA technology in maize breeding. VI. Foreign gene influence on maize genome expression after plant transformation by different technologies. - Genetika, Vol. 23, No. 3. 191-204.
Two technologies for foreign gene introduction into the maize genome have been applied: slightly modified pollen-tube pathway technology and dry seed incubation in plasmid DNA solution. Both gene introduction, integration and expression were followed in the plants of T1 and T2 generations. NPT II gene integration was followed by dot-blot and Southern blot analysis of plant DNA of T1 and T2 plants. Gene expression was analysed by neomycinphospho-transferase and several allozyme systems. Transformed plants contained the selective marker NPT II gene sequence in an active form. Bacterial gene integration induced several heritable changes of plant phenotype. As an important change, alteration of the flowering time has been used as a criterion for selection and plant propagation to keep transformed progeny. Besides plant genome transformation, endogenous bacteria living in different maize tissues were found. Enterobacter cloacae, Pseudomonas maltophylia, Acinetobacter calco-aceticus and Enterobacter aerogenes were detected both in control and transformed plants.
Gram positive Bacillus licheniformis was detected among bacteria living in all analysed tissues, except the endosperm. Experiments on detailed analysis of induced allozyme polymorphism, coupled to the specific site of gene integration in the maize genome are in progress.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 205-214
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.546.621.633.15:631.523.11
Original scientific paper
THE EFFECT OF ALUMINIUM ON GENE EXPRESSION IN MAIZE
Vesna LAZIĆ-JANČIĆ, D. KOVAČEVIĆ and M. DENIĆ
Maize Research Institute "Zemun Polje", 11080 Belgrade-Zemun, Yugoslavia
Abstract
Lazić-Jančić, V., D. Kovačević and M. Denić (1991): The effect of aluminium on gene expression in maize. - Genetika, Vol. 23, No. 3, 205-214.
The effect of Al stress on incorporation of 35 S-methionine into protein was examined in maize roots. Plants were grown in nutrient solution with or without 250 micromolar Al. Roots of intact plants were labeled in vivo and proteins were extracted and analyzed by fluorography of two-dimensional gels. Analysis of the proteins indicated that Al induced changes in the accumulation of soluble proteins, both after 2h of Al treatment (Al shock) and after 4d of treatment (Al grown). To determine whether or not these proteins were accumulated as a result of gene activation, RNA was extracted from control and treated roots and translated in vitro. The translation products were analyzed on gradient gels (10-20%) and compared with heat shock translation products.
The results showed that regulation of the synthesis of some proteins occurred at the level of mRNA accumulation. Comparison of the gene products after Al stress and heat shock did not suggest a specific response of maize inbred lines to Al stress.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 215-222
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.631.527.1
Original scientific paper
PLANT REGENERATION FROM CALLUS TISSUE CULTURES OF POPLARS
Z. TOMOVIĆ1 and Branka KOLEVSKA-PLETIKAPIĆ2
1 Poplar Research Institute, A. Čehova 13, 21000 Novi Sad
2 Department of Molecular Biology, Faculty of Science, University of Zagreb, Roosveltov trg 6, 41001 Zagreb, Yugoslavia
Abstract
Tomović, Z. and B. Kolevska-Pletikapić (1991): Plant regeneration from callus tissue cultures of poplars. - Genetika, Vol. 23, No. 215-222.
Plantlets were regenerated from callus tissue of the poplar hybrids Populus alba x P. grandidentata, P. alba x P. alba and from P. tricliocarpa and P. deltoides. Organogenic calli of poplar genotypes were obtained from leaves and stem segments excised from plants grown in vitro as well as from immature embryos. The possibility of shoot induction in callus cultures obtained from leaves and stem segments was limited by genotype - shoots were obtained from two of the six genotypes examined. In callus cultures obtained from immature embryos shoots were regenerated in all genotypes examined. In this callus the number of developed shoots was significantly higher. Shoots were successfully multiplied through auxiliary bud culture. Rooted plantelets were acclimatized in a greenhouse and then planted in the field.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 223-230
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.17.576.3.914.6
Original scientific paper
MORPHOLOGICAL GENETIC VARIABILITY IN FIVE NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER
Maria D. OCHANDO and C. DE JUANA
Departamento de Genetica, Facultad de Ciencias Biologicas
Universidad Complutense, 28040-Madrid, Spain
Abstract
Ochando, M.D.and C. de Juana (1991): Morphological genetic variability in five natural populations of Drosophila melanogaster. - Genetika, Vol. 23, No. 3 223-230.
It has been found in five natural populations of Drosophila melanogaster located in Spain that the frequencies of morphologically mutant phenotypes oscillate between 0.79 and 0.05 per captured individual.
The study of the inbred F2 progenies of the captured females shows that these females carry between 2.20 and 2.80 recessive mutants in the heterozygous condition.
The genetic variability discovered is discussed in relation to that observed by other authors and in relation to the population characteristics.
It would appear that the frequencies of recessive morphological mutants observed in our populations are totally compatible with the result of an equilibrium between mutation and selection against the recessive homozygotes.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 231-237
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.631.523.11: 635.15
Original scientific paper
DIALLEL ANALYSIS OF KERNEL MOISTURE CONTENT AFTER BLACK LAYER FORMATION IN MAIZE
L. KOJIĆ, I. HUSIĆ, R. STOJŠIN and G. DRINIĆ
Maize Research Institute "Zemun Polje", 11080 Belgrade-Zemun, Yugoslavia
Abstract
Kojić L., I. Husić, R. Stojšin and G. Drinić (1991): Diallel analysis of kernel moisture content after black layer formation in maize. - Genetika, Vol. 23, No. 3, 231-237.
In a diallel series of five inbred lines, an analysis of variance of combining value for seed water content, 10 and 20 days after black layer formation, showed highly significant differences for general (GCA) and specific (SCA) combining ability.
The GCA/SCA ratio was slightly greater than one and showed a prevalence of additive over non-additive gene action. Inbred lines Va25, R59 and Oh43ht had the highest GCA value. Opposite to this, inbred lines B432 and VI58 had negative GCA values and are therefore interesting from the viewpoint of maize improvement for a smaller seed water content.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 239-245
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.631.523.11 : 633.15
Original scientific paper
GENE EFFECTS FOR EAR LENGTH IN B73 AND B84 CROSSES OF MAIZE (ZEA MAYS L.)
J. ĐORĐEVIĆ and M. FILIPOVIĆ
Maize Research Institute "Zemun Polje" 11080 Belgrade-Zemun, Yugoslavia
Abstract
Đordeviđ, J. and M. Filipović (1991): Gene effects for ear length in B73 and B84 crosses of maize (Zea mays L.) - Genetika, Vol. 23, No. 3, 239-245.
In this paper the effect of genes for ear length was investigated in two high yielding maize hybrids with a common parent. The interdependence of gene effects and phenotypic expression of ear length as a desirable agronomic trait was investigated. In that case it is possible to determine type of gene effects which make a greater contribution to the inheritance of ear length.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 247-258
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.631.523.11: 634.2
Original scientific paper
CYTOGENETIC PROPERTIES OF SOUR CHERRY IN RELATION TO POLLEN
R. CEROVIĆ
Fruit and Viticulture Research Institute, 32000 Čačak, Yugoslavia
Abstract
Cerović, R. (1991): Cytogenetic properties of sour cherry in relation to pollen. - Genetika, Vol. 23, No. 3, 247-258.
Studies were conducted on the cytogenetic characteristics-conjugation of chromosomes, their lagging and the tetrad stage in two sour cherry (Prunus cerasus L.) cultivars in relation to viability and pollen germination in vitro. The results show that the varying degree of meiotic irregularities during microsporogenesis in sour cherries tested in individual years is correspondingly correlated with pollen viability and germination in vitro. This correlation is more expressed in 'Čačanski Rubin' than 'Šumadinka'.
Published in „GENETIKA“ Vol. 23, No.3 (1991), pp. 259-270
© 1991 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.631.523 : 634.84
Original scientific paper
A NEW GRAPE VARIETY RESISTANT TO PLASMOPORA VITICOLA
P. CINDRIĆ, Nada KORAĆ and Mira MEDIĆ
Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia
Abstract
Cindrić, P., N. Korać and M. Medić (1991): A new grape variety resistant to Plasmopora viticola, - Genetika, Vol. 23, No. 3, 259-270.
The prospective table grape variety Lasta was made by crossing the French variety Muscat de Saint Vallier and the Soviet variety Ljana. Both parents are table varieties resistant to the fungal disease caused by Plasmopara viticola. The new variety inherited tlus trait from the parents but outclassed them in grape quality. The variety matures approximately at the same time as Muscat Hamburg. Grape bunches can stay on the vine for an extended period. The bunch is attractive, with oval yellow berries of uniform size. The berries are firm, with a crunchy consistency, and they give a lasting refreshment. The variety yields a large quantity of commercial table grapes. In the test, the new variety was compared with the parents and the variety Muscat Hamburg.