GENETIKA, Vol

GENETIKA, Vol. 24, No.2 (1992)

M. ANDJELKOVIĆ and D. MARINKOVIĆ

ALLOZYME POLYMORPHISM OF DROSOPHILA SUBOBSCURA SELECTED FOR COPULATION ABILITY IN THE ABSENCE OF LIGHT [Abstract]

Milica MARJANOVIĆ, D. MARINKOVIĆ and Stefanija MARKOVIĆ

POPULATION GENETIC STUDY OF DIABETES MELLITUS [Abstract]

Olivera MILOŠEVIĆ-ĐORĐEVIĆ and B. GARZIČIĆ

REACTION OF HUMAN T LYMPHOCYTES ON CYTOSTATIC EFFECTS IN VITRO

I. MUTAGENIC EFFECTS OF DEGRANOL [Abstract]

Olivera MILOŠEVIĆ-ĐORĐEVIĆ and B. GARZIČIĆ

REACTION OF HUMAN T LYMPHOCYTES TO CYTOSTATIC EFFECTS IN VITRO

II. CITOZAR [Abstract]

Smiljana RISTIĆ, R. HADŽISELIMOVIĆ and D. MARINKOVIĆ

POPULATION-GENETIC ANALYSIS OF SOME PHENOTYPIC SYSTEMS OF QUALITATIVE VARIATION IN THE POPULATION OF RIJEKA [Abstract]

G. CEKUŠ

THE OCCURRENCE OF THE FOSSA MENTALIS IN FOUR REPRESENTATIVE GROUPS FROM NORTH-BAČKA [Abstract]

R. PETROVIĆ, M. FILIPOVIĆ and M. VIDAKOVIĆ

IDENTIFICATION OF SOURCES CONTAINING USEFUL ALLELES FOR IMPROVING PARENTS OF SUPERIOR MAIZE SINGLE CROSSES (Zea mays L.) [Abstract]

R. KRISHNA and Z. AHMAD

HETEROSIS FOR YIELD COMPONENTS AND DEVELOPMENTAL TRAITS IN SPRING WHEAT [Abstract]

B. JOVANOVIĆ, Radojka MALETIĆ and S. PRODANOVIĆ

APPLICATION OF THE HIERARCHICAL CLUSTER METHOD IN ESTIMATES OF GENETICAL DIVERGENCE AMONG SOYBEAN CULTIVARS [Abstract]

M. DIMITRIJEVIĆ and Marija KRALJEVIĆ-BALALIĆ

COMBINING ABILITY FOR NUMBER OF KERNELS PER SPIKE IN WHEAT [Abstract]

Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 73-79

Serbia

UDC 575.2

Original scientific paper

ALLOZYME POLYMORPHISM OF DROSOPHILA SUBOBSCURA SELECTED FOR COPULATION ABILITY IN THE ABSENCE OF LIGHT

M. ANDJELKOVIĆ¹ and D. MARINKOVIĆ²

¹Institute for Biological Research and Faculty of Science, University of Belgrade, 11060 Belgrade, Yugoslavia

²Faculty of Science, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Andjelković, M. and D. Marinković (1992): Allozyme polymorphism of Drosophila subobscura selected for copulation ability in the absence of light. - Genetika, Vol. 24, No. 2, 73 - 79.

In a D. subobscura line selected over thirty generations for copulation ability in the absence of light, the electrophoretic analysis of Ao-2, Est-5, Gpd-3, Lap-4 and Pgm-1 loci registered an allozyme polymorphism in Est-5 and Lap-4 genes. The deviation from theoretically expected genotypic frequencies is significant, suggesting that multigenerational selection for this type of reproductive behavior led to a change of genetic homeostasis, which could be a prerequisite for such a positive selection.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 81-88

Serbia

UDC 578.084

Original scientific paper

POPULATION GENETIC STUDY OF DIABETES MELLITUS

Milica MARJANOVIĆ¹, D. MARINKOVIĆ² and Stefanija MARKOVIĆ³

¹Medical Center New Belgrade, 11070 Belgrade, Yugoslavia

²Faculty of Science, University of Belgrade, 11000 Belgrade, Yugoslavia

³Faculty of Medicine, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Marjanović, M., D. Marinković and S. Marković (1992): Population genetic study of diabetes mellitus. - Genetika, Vol. 24, No. 2, 81-88.

An analysis of individual variation among pregnant women with and without diabetes mellitus in Belgrade, Yugoslavia, has shown manifold differences between these two samples of individuals, and among their relatives. Diabetic women had a significantly increased amount of homozygously recessive traits; the ARh + blood type was more and ORh + less frequently present among them; their relatives had an increased frequency of d. mellitus in comparison to relatives of non-diabetics.

A set of diabeetogenic factors was also determined more frequently among women with d. mellitus (such as stress, chemical and physical factors, previous diseases, obesity, etc.). An increased risk for the progeny of diabetics was also detected, with an accumulation of genetic and congenital influences.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 89-94

Serbia

UDC 578.083

Original scientific paper

REACTION OF HUMAN T LYMPHOCYTES ON CYTOSTATIC EFFECTS IN VITRO

I. MUTAGENIC EFFECTS OF DEGRANOL

Olivera MILOŠEVIĆ-ĐORĐEVIĆ¹ and B. GARZIČIĆ²

¹Faculty of Science, University of Kragujevac, 34000 Kragujevac
²Institute of Oncology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Milošević-Đorđević, O. and B. Garzičić (1992): Reaction of human T Lymphocytes on cytostatic effects in vitro. I. Mutagenic effects of degranol. - Genetika, Vol. 24, No. 2, 89 - 94.

To determine the effect of alkylating agents on malignant and normal cells, we tested the effect of Degranol on human T lymphocytes in differentiated and non-differentiated states in an in vitro system. The method of Edwards and Frland somewhat modified was used and Degranol was dissolved in the medium and added to cell cultures in two ways: 1-24 hours before incubation of cultures with phytohemaglutinin, and H-24 hours before the end of incubation, acting simultaneously with phytohemaglutinin. The deleterious effect of Degranol was determined by analysing chromosomal aberrations. A significant increase was noticed in the frequency of chromosomal aberrations in the analyses of treated differentiated and non-differentiated cells, in relation to the control group of untreated cells (233.8% and 80.8%). It may be concluded that the alkylating agent Degranol produced such changes independently of the cell cycle phase, and with equal damage to malignant and healthy cells of the organism.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 95-100

Serbia

UDC 578.083

Original scientific paper

REACTION OF HUMAN T LYMPHOCYTES TO CYTOSTATIC EFFECTS IN VITRO

II. CITOZAR

Olivera MILOŠEVIĆ-ĐORĐEVIĆ¹ and B. GARZIČIĆ²

¹Faculty of Science, University of Kragujevac, 34000 Kragujevac

²Institute of Oncology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Milošević-Đorđević, O. and B. Garzičić (1992): Reaction of human T lymphocytes to cytostatic effects in vitro. II. Citozar. -Genetika, Vol. 24, No. 2, 95 -100.

The effect of the analog DNA Citozar was tested on differentiated (healthy cell) and undifferentiated (malignant cell) human T lymphocytes. The method of Edwards and F r 1 a n d, somewhat modified was used and Citozar was added in the con. 10^-5 M to cell cultures in two ways: 1-24 hours before incubation of cultures with PHA (differentiated states) and 11-24 hours before the end incubation with PHA (undifferentiated states). The effect of Citozar was determined by analysing the percent chromosomal aberrations. A significant increase was noticed in the frequency of chromosomal aberrations in the analyses of treated undifferentiated cells in relation to treated differentiated and untreated control cells. The data obtained, show the selective actions of the antimetabolite Citozar on differentiated and undifferentiated human T lymphocytes.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 101-107

Serbia

UDC 577.25

Original scientific paper

POPULATION-GENETIC ANALYSIS OF SOME PHENOTYPIC SYSTEMS OF QUALITATIVE VARIATION IN THE POPULATION OF RIJEKA

Smiljana RISTIĆ¹, R. HADŽISELIMOVIĆ² and D. MARINKOVIĆ³

¹Department of Biology, University of Rijeka, Rijeka, 51000 Rijeka, Hrvatska

²Faculty of Natural and Mathematical Sciences, University of Sarajevo, 71000 Sarajevo, Bosnia and Hercegovina

³Faculty of Biological Science, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Ristić, S., R. Hadžiselimović and D. Marinković (1992): Population-genetic analysis of some phenotypic systems of qualitative variation in the population of Rijeka. - Genetika, Vol. 24, No. 2, 101 -107.

A population-genetic analysis of 11 morphological and 5 biochemical and physiological systems was carried out in 817 school children from Rijeka. The frequencies of the majority of recessively controlled morphological traits varied similarly to those of other European populations. Among physiological phenotypes, the presence of PTC-tasters was somewhat greater than in the majority of populations from our Continent. Significant differences were found in a few traits between boys and girls, such as in deuterodefects and in the frequency of hair phenotypes.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 109-114

Serbia

UDC 575.1

Original scientific paper

THE OCCURRENCE OF THE FOSSA MENTALIS IN FOUR REPRESENTATIVE GROUPS FROM NORTH-BAČKA

G. CEKUŠ

Teacher's College, 24000 Subotica, Yugoslavia

Abstract

Cekuš, G. (1992): The occurrence of the fossa mentalis in four representative groups from North-Bačka. - Genetika, Vol. 24, No. 2, 109-114.

The anthropological and genetic characteristics of the population of North-Bačka are less well-known. That is why we found it important to deal with this question. We observed all the pupils in each settlement investigated. The author examined the degree of occurrence of fossa mentalis in 1266 pupils. The degree of occurrence of the fossa mentalis oscillated between 7.31 and 18.55%. The difference between age groups was big but it varied irregularly. The longitudinal depression was more frequent in all experiments. The frequency of the corresponding recessive gene (g_ch) is approximately identical. It fluctuated between 0.90 and 0.96. The difference between the sexes was similarly small (in boys 0.94 and in girls 0.95).

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 115-126

Serbia

UDC 575.17:633.15

Original scientific paper

IDENTIFICATION OF SOURCES CONTAINING USEFUL ALLELES FOR IMPROVING PARENTS OF SUPERIOR MAIZE SINGLE CROSSES (Zea mays L.)

R. PETROVIĆ, M. FILIPOVIĆ and M. VIDAKOVIĆ

Maize Research Institute "Zemun Polje", 11080 Belgrade-Zemun, Yugoslavia

Abstract

Petrović, R., M. Filipović and M. Vidaković (1992): Identification of sources containing useful alleles for improving parents of superior maize single crosses (Zea mays L.). - Genetika, Vol. 24, No. 2,115-126.

The majority of the presently grown maize varieties has been developed by crossing inbred lines of maize.

In the search for a measure to estimate the relative value of parental inbred lines in maize (Zea mays L.), the general (OCA) and specific (SCA) combining ability, variance components, relative number of favourable dominant and unfavourable recessive alleles, relationship value and coefficient of rank correlation were estimated for grain yield, plant and ear length, number of kernels per row and ear length.

The total genetic variation in terms of coefficient of variation (CV) ranged from 28% for ear height to 50% for grain yield. The ratios of twice the general combining ability (GCA)/total genetic variance ranged from 0.46 for ear height to 0.92 for ear length. Hence, degree of prediction of relative parental value on the basis of GCA only, varied from trait to trait. The results obtained on the basis of predicting the relative values of the loci in donor lines revealed that donor lines, as potential carriers of the desired alleles for the improvement of given lines, differed.

Similarity in genetic constitution of loci between some inbreds, obtained by comparing relative locus values was in agreement with the origin of inbreds.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 127-132

Serbia

UDC 575.17:633.11

Original scientific paper

HETEROSIS FOR YIELD COMPONENTS AND DEVELOPMENTAL TRAITS IN SPRING WHEAT

R. KRISHNA and Z. AHMAD

Department of Genetics and Plant Breeding, C.S. Azad University of Agriculture and Technology, Kanpur - 206 002, India

Abstract

Krishna, R. and Z. Ahmad (1992): Heterosis for yield components and developmental traits in spring wheat. - Genetika, Vol. 24, No. 2,127-132.

In 72 F₁ s involving 22 diverse genotypes (18 as lines and 4 as testers) of spring wheat heterotic effects over the better parents and economic parent (Sonalika), respectively were analysed for grain yield, its components and some developmental attributes. Highest mean heterosis was obtained for grain weight per spike (26.78%) followed by 1000-grain weight (14.60%), grain yield (12.52%), harvest index (9.72%) and grains per spike (8.59%). Forty six crosses gave significantly higher yields than their respective better parents. Forty two crosses exhibited significantly higher yields than the economic parent. Grain weight per spike, 1000-grain weight, harvest index and grains per spike made the greatest contribution towards the increase in grain yield. Developmental attributes, specially early growth vigour, senescence of leaves and plant height moved in a desirable direction in some of the high yielding heterotic hybrids. Generally, negative heterosis was recorded for productive tillers and spike length. The best F₁ s involved parents of high x high, high x low and low x low performance for yield, its component traits and developmental traits.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 133-138

Serbia

UDC 575.1:633.3

Original scientific paper

APPLICATION OF THE HIERARCHICAL CLUSTER METHOD IN ESTIMATES OF GENETICAL DIVERGENCE AMONG SOYBEAN CULTIVARS

B. JOVANOVIĆ, Radojka MALETIĆ and S. PRODANOVIĆ

Institute of Field Crops, Faculty of Agriculture, University of Belgrade, 11080 Belgrade - Zemun, Yugoslavia

Abstract

Jovanović, B., R. Maletć and S. Prodanović (1992): Application of the hierarchical cluster method in estimates of genetical divergence among soybean cultivars. - Genetika, Vol. 24, No. 2,133 -138.

Germplasm collection of 20 soybean varieties was examined in a three year trial. Measurements and registrations of eleven quantitative characters were made, including plant height, lowest pod height, number of fertil nodes on the main stem, number of pods per node, number of pods per plant, number of seeds per plant, number of seeds per pod, seed yield per plant, 100 dm³ seed weight, 1000 seed weight and oil content.

The genetical divergence among the soybean cultivars studied was analyzed by the hierarchical cluster method which is very useful in revealing germplasm groupings. Eight clusters were delineated among the 20 soybean genotypes. Soybean genotypes included within the same cluster group had similar wide ranges of variation for the quantitative characters studied and a low degree of genetical diversity. Sampling from different clusters had high allelic diversity. Thus, cluster analysis provides useful information for programs of soybean breeding.

This work is one of the first investigations of germplasm resource banks in our country.

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Published in „GENETIKA“ Vol. 24, No.2 (1992), pp. 139-144

Serbia

UDC 575.2:633.11

Original scientific paper

COMBINING ABILITY FOR NUMBER OF KERNELS PER SPIKE IN WHEAT

M. DIMITRIJEVIĆ and Marija KRALJEVIĆ-BALALIĆ

Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia

Abstract

Dimitrijević, M. and M. Kraljević-Balalić (1992): Combining ability for number of kernels per spike in wheat. - Genetika, Vol. 24, No. 2,139-144.

The mode of inheritance for the number of kernels per spike in wheat was studied in this paper. Six different genotypes of common wheat were evaluated in diallel crosses excluding reciprocals, and F₁ generations were obtained. The results revealed additive and non-additive gene action in the inheritance of kernel number per spike. The best general combiner for the examined trait was Erect RAF E-2, as well as NS12-77, which proved to be a good and stable general combiner.

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