GENETIKA, Vol. 24, No.3 (1992)
Kosana KONSTANTINOV, Snežana MLADENOVIĆ, Bojana TADIĆ, Vesna ŠUKALOVIĆ and J. DUMANOVIĆ
INFLUENCE OF FOREIGN GENES ON ALLOZYME PROFILES IN TRANSGENIC MAIZE PLANTS [Abstract]
Branka B. LOTHROP, Dragana RADOVIĆ, Ljiljana VAPA, Zorana BANOVAČKI and A. VUJIĆ
MORPHOLOGICAL AND ISOZYME VARIABILITY OF ISOLATED POPULATIONS OF THE SPECIES AEDES COMMUNIS (DEGEER, 1776) (DIPTERA: CULICIDAE) [Abstract]
Snežana MEZEI and L. KOVAČEV
REGENERATION OF PLANTS FROM CALLUS TISSUE OF SUGAR BEET (BETA VULGARIS L.) [Abstract]
Ljiljana VAPA, R. KASTORI and B. BAI-ZHANG
PROTEIN VARIABILITY OF SOME CHINA-GROWN WHEAT CULTIVARS [Abstract]
Ljiljana VAPA, B. ANDRIĆ, Marija KRALJEVIĆ-BALALIĆ and M. JOST
INHERITANCE OF HIGH MOLECULAR WEIGHT GLUTENINS IN WHEAT (TRITICUM AESTIVUM L.) [Abstract]
G. DRINIĆ, L. KOJIĆ, M. BABIĆ and Jelena VANČETOVIĆ
MULTIPLE REGRESSION ANALYSIS FOR GRAIN YIELD AND FOUR AGRONOMICALLY IMPORTANT TRAITS OF MAIZE (ZEA MAYS L.) [Abstract]
Jelena VANČETOVIĆ, G. DRINIĆ and M. BABIĆ
STABILITY PARAMETERS FOR GRAIN YIELD OF TOP-CROSS MAIZE HYBRIDS (ZEA MAYS L.) [Abstract]
V. ISAJEV and I. DORMING
PHOTOPERIODIC CONTROL OF GROWTH AND GROWTH CESSATION IN 30 HALF SIB FAMILIES OF SERBIAN SPRUCE SEEDLINGS (PICEA/OMORIKA/PANC./PURKYNE) [Abstract]
M. KUBUROVIĆ, M. PAVLOVIĆ and M. MILOVANOVIĆ
INHERITANCE OF PRIMARY ROOT NUMBER IN WINTER WHEAT [Abstract]
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 145 - 161
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.17.635.15
Original scientific paper
INFLUENCE OF FOREIGN GENES ON ALLOZYME PROFILES IN TRANSGENIC MAIZE PLANTS
Kosana KONSTANTINOV, Snežana MLADENOVIĆ, Bojana TADIĆ, Vesna ŠUKALOVIĆ and J. DUMANOVIĆ
Maize Research Institute "Zemun Polje" 11081 Zemun - Belgrade,
Yugoslavia
Abstract
Konstantinov, K., S. Mladenović, B. Tadić, V. Šukalović and J. Dumanović (1992V Influence of foreign genes on allozyme profiles in transgenic maize plants. - Genetika Vol. 24, No. 3, 145 - 161.
Transgenic maize plants were obtained by dry seed incubation in plasmid DNA solution containing the NPT II gene and by the pollen gene tagging system using the same gene. Introduced gene expression (neomycinphosphotransferase activity), gene integration (Southern blot analysis) and gene transmission were confirmed both in T1 and T2 transgenic plants of the B73 maize inbred line. Bacterial DNA integration induced numerous phenotypic changes which were eliminated through the cell cycle. Plant height alternation and reduction of the period between planting and flowering are inheritable traits and no segregation was obtained in the T2 and T3 generations. In this paper the results for T3 plants are not presented. The allozyme profiles were changed in transgenic plants at several of the 16 analyzed loci: Acpl, Acp4, Mdhl, Mdh2, Mdh5, BetaGlu, Idh2 and Pgm2. The results for specific activity changes of ACP and MDH in transgenic plants are discussed. As plant height and time flowering are important breeding traits, field trial testing of the combining ability was started during the summer of 1991.
The results obtained confirmed the possibility of transforming maize by foreign gene transfer during different developmental stages of the plant. The results also indicated that, besides foreign gene expression in the maize plant, the introduced DNA could induce mutations which change host genome functions.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 163 -171
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 576.31
Original scientific paper
MORPHOLOGICAL AND ISOZYME VARIABILITY OF ISOLATED POPULATIONS OF THE SPECIES AEDES COMMUNIS (D E G E E R, 1776) (DIPTERA: CULICIDAE)
Branka B. LOTHROP1, Dragana RADOVIĆ, Ljiljana VAPA, Zorana BANOVAČKI and A. VUJIĆ
1Coachella Valley Mosquito Abatement District, 83-733 Avenue 55, Thermal, CA 92274-9491, USA
Institute of Biology, Faculty of Sciences, 21000 Novi Sad, Yugoslavia
Abstract
Lothrop, B.B., D. Radović, Lj. Vapa, Z. Banovački and A. Vujić (1992): Morphological and isozyme variability of isolated populations of the species Aedes communis (De Geer, 1976) (Diptera: Culcidae). — Genetika, Vol. 24, No. 3,163 -171.
The variability of populations of the species Aedes communis (D e Geer, 1776) from the mountains Kopaonik and Durmitor (Yugoslavia) was analysed by morphological and genetic studies. Morphological analysis showed no significant differences between populations. Gene-enzyme variations were used to calculate heterozygosity (H), polymorphism (P), genetic identity (I) and genetic distance (D). Values of genetic identity (I) and distance (D) show that the populations examined are in the initial phase of speciation at the subspecies level.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 173-179
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 633.413
Original scientific paper
REGENERATION OF PLANTS FROM CALLUS TISSUE OF SUGAR BEET (BETA VULGARIS L.)
Snežana MEZEI and L. KOVAČEV
Faculty of Agriculture, Institute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia
Abstract
Mezei, S. and L. Kovacev (1992): Regeneration of plants from callus tissue of sugarbeet (Beta vulgarisL.), - Genetika, Vol. 24, No. 3., 173-179.
The effects of genotype, explain origin, and duration of subculturing on callus proliferation were tested in five sugar beet genotypes (Beta vulgaris L.). Calli were induced from explants of the leaf, hypo-cotyl and cotyledon segments. The experiment was set up in a three-factorial design. Three measurements were made. Adventitious buds were successfully induced on the calli cultured in the medium with 4.6 µM kinetin and that with 4.4 µM BA and 53.7 µM NAA. The calli maintained organogenic capacity for eight months. Two clone populations were established, 41 L3K and 75 L6K. Cytological analysis of plants obtained by organogenesis indicated the presence of polyploidy and polytheny in the course of endomitosis.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 181 -187
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 633.11:631.526
Original scientific paper
PROTEIN VARIABILITY OF SOME CHINA-GROWN WHEAT CULTIVARS
Ljiljana VAPA1, R. KASTORI2 and B. BAI-ZHANG3
1Faculty of Sciences 21000 Novi Sad, Yugoslavia
2Faculty of Agriculture, 21000 Novi Sad, Yugoslavia
3Jilian Agricultural University, Changchun, PR China
Abstract
Vapa, Lj., R. Kastori and B. Bai-zhang (1992): Protein variability of some China-grown wheat cultivars. - Genetika, Vol. 24, No. 3,181 -187.
The storage proteins of 33 China-grown spring wheat cultivars were separated by SDS-PAG electrophoresis to determine their HMW glutenin alleles. Alleles 1, 2* and N were identified, at the GIu-Al locus alleles 7,7+9 and 7+8 at the Glu-Bl locus and alleles 2+12,5+10 and 5+12 at the Glu-Dl locus. The most frequent HMW glutenin composition was obtained for alleles N, 7+8, 2+12. Two cultivars were found to consist of biotypes with different allels. Grain protein content ranged from 11.99 to 16.54%, and significant differences were found between the cultivars examined.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 189 -194
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 633.11
Original scientific paper
INHERITANCE OF HIGH MOLECULAR WEIGHT GLUTENINS IN WHEAT (TRITICUM AESTIVUM L.)
Ljiljana VAPA1, B. ANDRIĆ1, Marija KRALJEVIĆ-BALALIĆ2 and M. JOST3
1Faculty of Sciences, 21000 Novi Sad, Yugoslavia
2Faculty of Agriculture, 21000 Novi Sad, Yugoslavia
3Institute of Agriculture, 43000 Križevci, Croatia
Abstract
Vapa, Lj., B. Andrić, M. Kraljević-Balalić and M. Jost (1992): Inheritance of high molecular weight glutenins in wheat (Triticum aestivum L.). - Genetika, Vol. 24, No. 3,189 -194.
The inheritance of high molecular weight (HMW) glutenin subunits in wheat was analysed by using sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Plants having different glutenin alleles encoded by the Glu-Al, Glu-B1 and Glu-D1 loci were crossed and their F1and F2 generations were examined. The results obtained showed that F1 seeds contained the HMW glutenin subunits characteristic for both parents. In the F2 generation HMW glutenin subunits segregated in accordance with control by a single gene with a dominant or codominant effect.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 195 - 200
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 635.15:631.52
Original scientific paper
MULTIPLE REGRESSION ANALYSIS FOR GRAIN YIELD AND FOUR AGRONOMICALLY IMPORTANT TRAITS OF MAIZE (ZEA MAYS L.)
G. DRINIĆ, L. KOJIĆ, M. BABIĆ and Jelena VANČETOVIĆ
Maize Research Institute "Zemun Polje" 11080 Belgrade-Zemun, Yugoslavia
Abstract
Drinić G., L. Kojić, M. Babić and J. Vančetović (1992): Multiple regression analysis for grain yield and four agronomically important traits of maize (Zea mays L.) - Genetika, Vol. 24, No. 3, 195 - 200.
This paper deals with the calculation and testing of multiple regression and path coefficient analyses for grain yield as a dependent variable and index leaf angle, plant height, kernel row number and ear length as independent variables. Ten F1 maize hybrids developed from diallel crosses among five inbred lines were analyzed.
The highest correlation was found between plant height and grain yield per plant. Though the direct effect of ear length on grain yield was negative, the indirect effect of this trait on grain yield via plant height was higher and positive.
The high values of the determination coefficients obtained indicate that the investigated traits have significant effects on grain yield in maize.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 201 - 208
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 635.15
Original scientific paper
STABILITY PARAMETERS FOR GRAIN YIELD OF TOP-CROSS MAIZE HYBRIDS (ZEA MAYS L.)
Jelena VANČETOVIĆ, G. DRINIĆ and M. BABIĆ
Maize Research Institute "Zemun Polje" 11080 Belgrade - Zemun, Yugoslavia
Abstract
Vančetovic, J., G. Drinić and M. Babić (1992): Stability parameters for grain yield of top-cross maize hybrids (Zea mays L.). - Genetika, Vol. 24, No. 3,201 - 208.
Stability parameters for grain yield of 36 top-cross maize hybrids have been investigated. The purpose was to determine yield stability of top-cross maize hybrids developed by crossing nine local varieties with four maize inbred lines (testers). These top-crosses were primarily developed in order to investigate the combining ability of local maize varieties from Yugoslavia.
The method of Eberhart and Russell (1966) was used for evaluation of grain yield stability parameters. Also, Spearman's rank correlation was done for yield of top-cross hybrids among different inbred lines (testers).
Significant genotype x location interaction in the analysis of variance indicated that top-cross hybrids differed in response to different environments. The stability parameter bi was more important than S2di in the top-cross hybrids investigated.
Three varieties were chosen for further research according to the yield of top-cross hybrids (i.e. combining ability) and values for the stability parameters.
The highest yields were obtained by crosses with inbred line B73. On the basis of the rank correlation it could be concluded that this line could replace all other testers in the trial.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 209-217
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 631.523
Original scientific paper
PHOTOPERIODIC CONTROL OF GROWTH AND GROWTH CESSATION IN 30 HALF SIB FAMILIES OF SERBIAN SPRUCE SEEDLINGS (PICEA/OMORIKA/PANC./PURKYNE)
V. ISAJEV1 and I. DORMING2
1Faculty of Forestry, 11030 Beograd, Yugoslavia
2SLU - Fitotronen, 104 05 Stockholm, Sweden
Abstract
Isajev, V. and I. Dormling (1992): Photoperiodic control of growth and growth cessation in 30 half sib families of Serbian spruce seedlings (Picea omorika/Panc./Purkyne) - Genetika, Vol. 24, No. 3, 209-217.
The first scientific information about variability in the photo-periodic reactions of 30 Serbian-spruce half-sib families from three plantations from West Serbia (Yugoslavia), is presented. The dependence of Serbian spruce shoot growth upon the length of the dark period was experimentally demonstrated. At the day length of 10 -12 hours, shoot growth cased and the shoots formed their terminal buds, so the family started its dormancy. With the prolongation of day length, growth was continued. The optimal effect on the growth of shoots was obtained by a day length of 18 to 20 hours. As this effect is conditioned by visible light, it is the case of a true photoperiodic effect on Serbian spruce. Half-sib families reacted differently to the changes of photoperiods, and the great intrafamily variability was the consequence of a high degree of species heterozygosity.
Published in „GENETIKA“ Vol. 24, No.3 (1992), pp. 219-225
© 1992 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 631.527.12:633.11
Original scientific paper
INHERITANCE OF PRIMARY ROOT NUMBER IN WINTER WHEAT
M. KUBUROVIĆ, M. PAVLOVIĆ and M. MILOVANOVIĆ
Institute for Research in Agriculture "Srbija", Center for Small Grains, 34000 Kragujevac, Yugoslavia
Abstract
Kuburović, M., M. Pavlović and M. Milovanović (1992): Inheritance of primary root number in winter wheat. - Genetika, Vol. 24, No. 3., 219-225.
The inheritance of primary root number in F1 and F2 generations from diallel crossing of 4 winter wheat varieties ('Sava', 'Libel-lula', 'Bankut-1205' and 'Kruševačka 9083'), was investigated in 21 day old plants. The number of primary roots is inherited partially dominant and intermediate. Parental varieties differed in primary root number. Thus, it was the greates for 'Sava' and the lowest for 'Kruševačka 9083'. The number of primary roots is controlled by additive and nonadditive effects of genes. 'Libellula' and 'Sava' had more recessive genes than 'Kruševačka 9083' and 'Bankut-1205' which had more dominant genes in the F1 generation. In the F2 generation 'Sava', 'Bankut-1205' and 'Kruševačka 9083' had similar recessive-dominant gene proportions, but 'Libellula' had more recessive genes. Heritability in the broad sense was very high (91%).