GENETIKA, Vol

GENETIKA, Vol. 25, No.3 (1993)

I. HUSIĆ, O. STOJNIĆ, M. IVANOVIĆ and L. KOJIĆ

MULTIPLE REGRESSION ANALYSIS IN CROSSES OF INBRED LINES DEVELOPED FROM DIFFERENT CYCLES OF SELECTION OF BSSS [Abstract]

R. MARINKOVIĆ, D. ŠKORIĆ and B. KRSTIĆ

MODE OF INHERITANCE OF DRY MATTER MASS OF ROOT, STEM AND LEAF IN F1 SUNFLOWER HYBRIDS (H. annuus L.) [Abstract]

Jelena VANČETOVIĆ and G. DRINIĆ

COMBINING ABILITY FOR YIELD COMPONENTS OF LOCAL AND SYNTHETIC POPULATIONS OF MAIZE (Zea mays L.) [Abstract]

Nevenka ALEKSIĆ

THE EFFECT OF LEVAMISOLE HYDROCHLORIDE ON HUMAN LYMPHOCYTE CULTURES [Abstract]

Olivera MILOŠEVIĆ-ĐORĐEVIĆ and N. BARJAKTAREVIĆ

REACTION OF HUMAN T LYMPHOCYTES TO CYTOSTATIC EFFECTS IN VITRO. III. VINBLASTIN AND COLCHICINE [Abstract]

Nevenka ALEKSIĆ and Nada BARJAKTAROVIĆ

INDUCTION OF SISTER CHROMATID EXCHANGE (SCE) BY IVERMECTIN [Abstract]

B. ĐORĐEVIĆ, H. MEISELMAN and W.F DEMAS

COMPARISON OF TWO METHODS OF SURVIVAL DETERMINATION IN X-IRRADIATED HYBRID SPHEROIDS [Abstract]

Zvezdana POPOVIĆ, Gordana NIKČEVIĆ. Suzana SAVKOVIĆ, Jelena MARJANOVIĆ and Sonja PAVLOVIĆ

TRANSCRIPTIONAL REGULATION OF THE β-GLOBIN GENE [Abstract]

M. VUJOŠEVIĆ

B-CHROMOSOMES IN MAMMALS [Abstract]

Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 177-182

Serbia

UDC 575.1: 635.15

Original scientific paper

MULTIPLE REGRESSION ANALYSIS IN CROSSES OF INBRED LINES DEVELOPED FROM DIFFERENT CYCLES OF SELECTION OF BSSS

I. HUSIĆ, O. STOJNIĆ, M. IVANOVIĆ and L. KOJIĆ

Maize Research Institute "Zemun Polje", 11080 Belgrade-Zemun, Yugoslavia

Abstract

Husić I., O.Stojnić, M. Ivanović, and L. Kojić (1993): Multiple regression analysis in crosses of inbred lines developed from different cycles of selection of BSSS. - Genetika, Vol. 25, No. 3, 177 - 182.

The test crosses of inbred lines developed from different cycles of BSSS (Iowa Stiff Stalk Synthetic) were used as the material to estimate the joint effects of yield components on grain yield. The main objective of this study was to determine the effect of recurrent selection for grain yield on correlation among traits using the coefficient of multiple determination (R2 y.i; i = l ... 5) of yield components on grain yield. Seven cycles of recurrent selection led to changes in the correlation traits. Coefficients of multiple determination of yield components on grain yield generally had higher values for crosses where one of the parents was B84 (BSSSC7) than for corresponding crosses where one of the parents was B37 (BSSS0). Kernel row number, number of kernels per row, and 1000 kernel weight had the highest joint effect on grain yield for both B84 and B37 crosses.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 183-191

Serbia

UDC 575.1: 633.85

Original scientific paper

MODE OF INHERITANCE OF DRY MATTER MASS OF ROOT, STEM AND LEAF IN F1 SUNFLOWER HYBRIDS (H. annuus L.)

R. MARINKOVIĆ¹, D. ŠKORIĆ¹ and B. KRSTIĆ²

¹Institute of Field and Vegetable Crops, 21000 Novi Sad

²Institute of Biology, 21000 Novi Sad, Yugoslavia

Abstract

Marinković R., D. Škorić and B. Krstić (1993): Mode of inheritance of dry matter mass of root stem and leaf in F1 sunflower hybrids (H. annuus L.). - Genetika Vol. 25. No. 3, 183 -191.

The paper deals with the mode of inheritance of dry matter mass (root, stem, leaf) in the F1 generation of sunflower dialleles of six genetically diverse genotypes (R-370, R-251, CMS-Ha-99, NS-B-16, M-6/4 and S-59)

In the F1 generation, the mode of inheritance of the traits studied depended on the crossing combination (intermediate, dominance of superior or inferior parent, positive or negative heterosis).

Additive and non-additive components of genetic variance played important roles in the inheritance of all three traits. The average degree of dominance, v H/D, was higher than unity, indicating that dry matter, when all combinations are taken into account, is inherited superdominantly.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 193-205

Serbia

UDC 575.1: 635.15

Original scientific paper

COMBINING ABILITY FOR YIELD COMPONENTS OF LOCAL AND SYNTHETIC POPULATIONS OF MAIZE (Zea mays L.)

Jelena VANČETOVIĆ and G. DRINIĆ

Maize Research Institute "Zemun Polje", 11080 Belgrade - Zemun, Yugoslavia

Abstract

Vančetović J. and G. Drinić (1993): Combining ability for yield components of local and synthetic populations of maize (Zea mays L.). - Genetika 25, No. 3, 193 - 205.

The combining abilities of eight maize populations (three synthetics and five local maize varieties) for four yield components were investigated. The populations chosen for this research were: local varieties under registry numbers 751, 1110b, 1583, 1571 and 1219 within the Variety collection of the Maize Research Institute ,,Zemun Polje", and synthetics ZPSIN1, ZPEP and BS13 (S2)C2. The method of diallel crossing, without reciprocals, was employed and Griffing' s method IV, mixed model B (1956) was used for the analysis of combining abilities. The yield components investigated were: ear length (cm), kernel row number, kernels per row and 1000 kernel weight (g).

The results showed that additive genetic effects had the highest influence on all investigated characters. SCA x location interaction was not significant for any trait, while GCAx location interaction was significant for all yield components.

The highest estimates of GCA effects in the following populations were found for the following traits:

- in 1110b, ZPSIN1 and BS13 for ear length.

- in ZPEP and BS13 for kernel row number,

- in three synthetics for kernels per row, and

- in 1110b, 1583 and 1571 for 1000 kernel weight.

Therefore, all the yield components investigated could be improved by using composites derived from combinations of the appropriate populations.

A negative correlation between GCA for 1000 kernel weight and kernel row number was observed.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 207-212

Serbia

UDC 612.42

Original scientific paper

THE EFFECT OF LEVAMISOLE HYDROCHLORIDE ON HUMAN LYMPHOCYTE CULTURES

Nevenka ALEKSIĆ

Faculty of Veterinary Medicine, University of Belgrade, 11000 Belgrade, Yugoslavia

Abstract

Aleksić N. (1993): The effect of levamisole hydrochloride on human lymphocyte cultures. - Genetika, Vol. 25, No. 3, 207 - 212.

The effect of levamisole hydrochloride on cultured PHA-activated human lymphocytes was investigated. Genotoxicity was evaluated by determination of the level of sister chromatid exchange (SCE). Mitomycin C was the positive control and DMSO (the substance in which the investigated antiparasitic was diluted) the negative control. Levamisole hydrochloride was tested in concentrations of 7.5, 15.0, 30.0, 60.0 and 120.0 μg/ml of culture medium. It increased the frequen¬cy of SCE in all doses applied. The difference was statistically significant in relation to the control at all doses except the lowest one.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 213-217

Serbia

UDC 612.42

Original scientific paper

REACTION OF HUMAN T LYMPHOCYTES TO CYTOSTATIC EFFECTS IN VITRO. III. VINBLASTIN AND COLCHICINE

Olivera MILOŠEVIĆ-ĐORĐEVIĆ and N. BARJAKTAREVIĆ

Faculty of Science, University of Kragujevac, 34000 Kragujevac

and

Belgrade Clinical Hospital, 11000 Belgrade, Yugoslavia

Abstract

Milošević-Đorđević O., and N. Barjaktarević (1993): Reaction of human T lymphocytes to cytostatic effects in vitro. III. Vinblastin and colchicine. - Genetika, Vol. 25, No. 3, 213 - 217.

The effect of inhibitors of the division spindle, vinblastin and colchicine was tested on normal cells in differentiated and non-differentiated states in a system in vitro. A modified method Edwards and Froland was used. Vinblastin (5µg/ml) and colchicine (1 µg/ml) were dissolved in the medium and added to cell cultures in two different ways: 1-24 hours before incubation of the culture with phytohema-glutinin; II - 3 hours before the end of incubation, so that they act simultaneously with phytohemaglutinin. Both differentiated and non-differentiated cells treated with vinblastin and colchicine showed a high mitotic index (M.I.). For vinblastin this was found to be 4% and 10% per individual, respectively, and for colchicine 8% and 7% per individual. The results obtained indicate that vinblastin and colchicine express a specific effect on a phase of the cell cycle, but the effect is independent of the phase in which the cell existed at the moment of treatment.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 219-225

Serbia

UDC 576.312

Original scientific paper

INDUCTION OF SISTER CHROMATID EXCHANGE (SCE) BY IVERMECTIN

Nevenka ALEKSIĆ and Nada BARJAKTAROVIĆ

Veterinary faculty, University of Belgrade, 11000 Belgrade

and

Clinical-hospital centre "Zvezdara", 11000 Belgrade, Yugoslavia

Abstract

Aleksić N. and N. Barjaktarović (1993): Induction of sister chromatid exchange (SCE) by ivermectin. - Genetika, Vol. 25, No. 3, 219-225.

The genotoxic effect of ivermectin, an antiparasitic drug, was investigated. It was tested in various concentrations in cultures of PHA-activated human lymphocyte, for the ability to induce sister chromatid exchange (SCE). In doses 0.30 µg/ml and higher it significantly raised the SCE level.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 227-231

Serbia

UDC 631.527.54

Original scientific paper

COMPARISON OF TWO METHODS OF SURVIVAL DETERMINATION IN X-IRRADIATED HYBRID SPHEROIDS

B. ĐORĐEVIĆ, H. MEISELMAN and W.F DEMAS

Department of Radiation Oncology, State University of New York, Health Science Center at Brooklyn, Brooklyn, NY 11203, U.S.A.

Abstract

Đorđević B., H. Meiselman and W.F Demas (1993): Comparison of two methods of survival determination in X-irradiated hybrid spheroids. - Genetika, Vol. 25, No. 3, 227 - 231.

A comparison between two methods of measuring survival of irradiated HeLa cells cultured in hybrid spheroids is presented. In one method, the average number of clonogenic cells per spheroid is deduced from the percentage of colony formers among surface-attached spheroids. Survival is calculated from the ratio of clonogenicitics in the treated series to that in the untreated series. The other method of survival measurement is based on direct counting of colonies obtained from equal numbers of trypsinized spheroids and relating the numbers in the two series, treated and control. Values for survival obtained by the two respective methods are very similar.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 233-245

Serbia

UDC 631.523.11

Original scientific paper

TRANSCRIPTIONAL REGULATION OF THE β-GLOBIN GENE

Zvezdana POPOVIĆ, Gordana NIKČEVIĆ. Suzana SAVKOVIĆ, Jelena MARJANOVIĆ and Sonja PAVLOVIĆ

Institute of Molecular Genetics and Genetic Engineering 11000 Belgrade,

Yugoslavia

Abstract

Popović Z., G. Nikčević, S. Savković, J. Marjanović and S. Pavlović (1993): Transcriptional regulation of the β-globin gene. - Genetika, Vol. 25, No. 3, 233 - 245.

We present recent data on the regulation of human mouse, rat and chicken adult β-globin gene transcription. Ubiquitous and erythroid specific cis-acting sequences found in promoter, core and 3' regulatory regions of the adult β -globin genes are enumerated. Control regions bind multiple proteins, consisting of tissue-specific and general factors, whose composition changes during erythroid maturation. Binding of erythroid specific: GATA-1, NF-E2, NF-E4 and erythroid nonspecific: TFIID, CP1, Spl, AP-1 and other proteins, is indicated. Finally, proposed models for protein - DNA action resulting in transcription regulation are considered.

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Published in „GENETIKA“ Vol. 25, No.3 (1993), pp. 248-258

Serbia

UDC 631.523.12

Review paper

B-CHROMOSOMES IN MAMMALS

M. VUJOŠEVIĆ

Dept. of Genetic, Institute for Biological Research "S. Stanković", 11060 Belgrade, Yugoslavia

Abstract

Vujošević M. (1993): B-chromosomes in mammals. - Genetika, Vol. 25, No. 3, 247 - 258.

B-chromosomes represent a very heterogeneous class of chromosomes whose main characteristic is that their presence is not essential for the survival of organisms which carry them. They are occur widely in plants and insects but have been found in only 34 species of mammals. B-chromosomes of mammals are characterized by the same features as are Bs in other organisms. They show great variability in their morphology, structure and meiotic behavior. Different types of intra and interpopulational variation are present including also intra individual variation. Although they all originate from chromosomes of the standard complement, their later destiny is quite different. Explanations about the mechanism of their maintenance in populations are not quite in agreement with existing models.

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