GENETIKA, Vol

GENETIKA, Vol. 28, No.3 (1996)

Marija KRALJEVIĆ-BALALIĆ

GENE EFFECT FOR PROTEIN CONTENT IN WINTER WHEAT [Abstract]

Duška STOJŠIN, Lyn W. KANNENBERG, Jarmila RAJNPREHT, Peter K. PAULS and Radomir STOJŠIN

GENETIC RELATIONSHIPS AMONG COMMERCIAL CORN HYBRIDS AND PARENTS BASED ON RAPD ANALYSES OF PERICARP AND EMBRYO DNA [Abstract]

Dragiša LOPANDIĆ, Marija KRALJEVIĆ-BALALIĆ, Mile IVANOVIĆ and Goran DRINIĆ

STABILITY PARAMETERS FOR YIELD OF MAIZE SYNTHETIC POPULATIONS OF DIFFERENT CYCLES OF SELECTION [Abstract]

Milomirka MADIĆ and Dragan ĐUROVIĆ

GENETIC ANALYSIS OF TILLERING IN BARLEY HYBRIDS [Abstract]

Gordana ŠURLAN-MOMIROVIĆ, Zorica TOMIĆ, Ivana RALEVIĆ1 and Nebojsa RALEVIĆ

EVALUATION OF A GERMPLASM COLLECTION OF AGROSTIS GIGANTEA ROTH. THROUGH A MULTIVARIATE ANALYSIS [Abstract]

Mirjana VASIĆ, Jelica GVOZDANOVIĆ-VARGA and Dušanka BUGARSKI

SEED YIELD PER PLANT IN WHITE BEAN CROSSES (PHASEOLUS VULGARIS L.) [Abstract]

Rakhee BANERJEE and B.N. SINGH

INTRASPECIFIC VARIATION IN THE NUMBER OF MALE SEX COMB TEETH IN DROSOPHILA BIPECTINATA [Abstract]

Olivera MILOŠEVIĆ-DJORDJEVIĆ, Dragoslav MARINKOVIĆ, and Slobodan ARSENIJEVIĆ

GENETIC DETERMINATION OF SISTER CHROMATID EXCHANGE (SCE) VARIATION IN NONSTIMULATED HUMAN LYMPHOCYTES AFTER TREATMENT WITH CYTOSTATICS IN VITRO [Abstract]

Snežana STANIĆ

THE MECHANISMS OF SEXUAL SELECTION OF Drosophila melanogaster [Abstract]

Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 129-135

Serbia

UDC 575.112:633.11

Original scientific paper

GENE EFFECT FOR PROTEIN CONTENT IN WINTER WHEAT

Marija KRALJEVIĆ-BALALIĆ

Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia

Abstract

Kraljević-Balalić M. (1996): Gene effect for protein content in winter wheat. - Genetika, Vol. 28, No. 3, 129 - 135.

The study was conducted to determine the inheritance, gene action and combining ability in 5 x 5 diallel wheat crosses. The parents and F₁ were analysed for soluble leaf protein content and grain protein content. The cultivars and their F₁ hybrids differed significantly in both examined characters. It was established that GCA variances were highly significant for both characters. For soluble leaf protein content there was preponderance of additive gene action, and for grain protein content a preponderance of non-additive gene action. Atlas 66 was a good general combiner for both characters.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 137-150

Serbia

UDC 633.15

Original scientific paper

GENETIC RELATIONSHIPS AMONG COMMERCIAL CORN HYBRIDS AND PARENTS BASED ON RAPD ANALYSES OF PERICARP AND EMBRYO DNA

Duška STOJŠIN^1,2, Lyn W. KANNENBERG¹, Jarmila RAJNPREHT¹, Peter K. PAULS¹ and Radomir STOJŠIN³

¹Department of Crop Science, University of Guelph, Guelph, Ontario,

²Maize Research Institute "Zemun Polje", 11081 Beograd, Yugoslavia

³First Line Seeds Ltd. R.R. #2, Guelph, ON N1H 6H8 Canada

Abstract

Stojšin D., L.W. Kannenberg, J. Rajnpreht, P. K. Pauls, and R. Stojšin (1996): Genetic relationships among commercial corn hybrids and parents based on RAPD analyses of pericarp and embryo DNA. -Genetika, Vol. 28, No. 3, 137 - 150.

A comprehensive evaluation of the relationship among commercial corn (Zea mays L.) hybrids requires the analysis of the parental inbred lines. However, the inbreds used in commercial hybrids are not publicly known and available. Considering that the hybrid pericarp tissue is of maternal origin, it should be possible to determine the genetic relationships of maternal inbred lines by analyzing hybrid pericarp DNA. The objectives of our study were to determine: (i) the relationship between embryo and pericarp DNA in corn hybrids; and (ii) genetic relationships among 28 commercial corn hybrids grown in Canada. The 28 hybrids (representing 11 corn companies) were chosen on the basis of their superior performance and/or because some general pedigree information was available to us. The embryo and pericarp DNAs were extracted from each hybrid and maternal inbreds of three of the hybrids (FX 010, FL Hades, and FL Tekila). Contrary to theoretical expectations, our results indicated that the RAPD (Random Amplified Polymorphic DNA) patterns obtained from hybrid pericarp DNA were not the same as those obtained from maternal inbred line embryo DNA. However, they were comparable to the markers obtained from the pericarp DNA of the maternal inbred line. Furthermore, the hybrid pericarp data generally agreed with the parental pedigree information available to us. Thus, hybrid pericarp DNA can he used for maternal inbred line identification. Hybrid pericarp DNA data, complemented by hybrid embryo DNA results, can be used in diversity studies as an indicator of relationships among hybrids and their parental inbred lines. To our knowledge, this is the first report of RAPD patterns from com pericarp DNA, and it represents a novel method of analyzing genetic relationships among inbred lines when only hybrid seeds are available. Our study also showed that diversity among commercial corn hybrids grown in Canada is limited and that similar inbred lines are frequently used as parents in more than one hybrid. Genetic similarities are found not only among hybrids developed by the same company, but also among hybrids with different brand names.

Key words: Zea mays L., RAPD markers, embryo and pericarp DNA, genetic relationships.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 151-158

Serbia

UDC 575:633.15

Original scientific paper

STABILITY PARAMETERS FOR YIELD OF MAIZE SYNTHETIC POPULATIONS OF DIFFERENT CYCLES OF SELECTION

Dragiša LOPANDIĆ¹, Marija KRALJEVIĆ-BALALIČ², Mile IVANOVIĆ³and Goran DRINIĆ³

¹ Agricultural Estate "Semberija", Bijeljina, Republic Srpska

²Faculty of Agriculture, Novi Sad, Yugoslavia

³ Maize Research Institute "Zemun Polje", Belgrade-Zemun, Yugoslavia

Abstract

Lopandić, D., M. Kraljević - Balalić, M. Ivanović and G. Drinić (1996): Stability parameters for yield of maize synthetic populations of different cycles of selection. - Genetika, Vol. 28, No. 3, 151 - 158.

The efficiency of HS and combined HS-FS i.e. S₁ recurrent selection was studied while improving grain yield and combining ability of two synthetic populations of different genetic background (ZPSynEP and ZPSynl). Stability parameters were determined after Eberhart and Russell (1966). The standard regression coefficient (b_i) in synthetics per se and test crosses points to stability of the traits, as its values did not significantly differ from unity. The value of this coefficient in the synthetic per se ZPSynl of Co-C₃ selection cycles decreased with each succeeding cycle of selection. The most stable yield of test crosses was found in ZPSynl C2xB84 (b_i=0.96). The increased grain yield of test crosses of succeeding cycles of synthetic selection was followed by their better response to more favourable environmental conditions.

Key words: maize, recurrent selection, synthetic populations, stability parameters.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 159-165

Serbia

UDC 575.3.633.16

Original scientific paper

GENETIC ANALYSIS OF TILLERING IN BARLEY HYBRIDS

Milomirka MADIĆ and Dragan ĐUROVIĆ

Faculty of Agronomy, 32000 Čačak, Yugoslavia

Abstract

Madić M. and D. Đurović (1996): Genetic analysis of tillering in barley hybrids. - Genetika, Vol. 28, No. 3, 159 - 165.

Four divergent barley genotypes underwent diallel crossing (excluding reciprocals) in order to assess the mode of inheritance, the effect of genes and the components of genetic variance in the F₁ generation. The mode of inheritance was assessed on the basis of the significance of differences between mean values for the hybrids in relation to the parental average. For the purpose of determining genetic variance components and the data of regression analysis, the method of M a t h e r and Jinks (1971) was used. The inheritance of tillering in the F₁generation was of intermediate type with partial dominance and overdominance. The additive variance component was higher than the values H₁and H₂, denoting that the genes with additive effects had a greater share in the inheritance of tillering. The average degree of dominance was less than 1, pointing to partial dominance in the inheritance of tillering. This was supported by the regression line intersecting the Wr-axis above the origin of the ordinale. The position of the points in the scatter diagram indicates that in the varieties Partizan and NS-293, genes with dominant effects are most numerous, whilst in the line KG-15, those with a recessive effect prevails. The best general combiner was the NS-293 cultivar, but the highest value of specific combining ability occurred in the NS-293 x KG-15 combination.

Key words: barley, tillering, gene effects, regression, cultivar, F₁hybrids.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 167-170

Serbia

UDC 575:519.237

Original scientific paper

EVALUATION OF A GERMPLASM COLLECTION OF AGROSTIS GIGANTEA ROTH. THROUGH A MULTIVARIATE ANALYSIS

Gordana ŠURLAN-MOMIROVIĆ¹, Zorica TOMIĆ², Ivana RALEVIĆ¹and Nebojsa RALEVIĆ¹

¹Faculty of Agriculture, University of Belgrade, Belgrade

²Institute "Serbia", Belgrade, Centre for Forage Cops, 37000 Kruševac, Yugoslavia

Abstract

Šurlan-Momirović G., Z. Tomić, I. Ralević, and N. Ralević (1996): Evaluation of a germplasm collection of Agrostis gigantea Roth, through a multivariate analysis. - Genetika, Vol. 28, No. 3, 167 - 170.

Fifteen autochthonous populations of the species Agrostis gigantea Roth. collected from a narrow Serbian region, were analyzed using both univariate and multivariate statistical analysis. In order to establish a taxonomic connection among germplasm accessions, similar populations were clustered in three groups. The plants were collected from various habitats: valley meadows 6 populations, upland meadows 3 populations and mountain meadows and pastures 6 populations. The nine characters were recorded during 1992, 1993 and 1994 on a single plain basis.

Key words: autochthonous populations, cluster analysis, univariate analysis.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 171-176

Serbia

UDC 631.524.635.65

Original scientific paper

SEED YIELD PER PLANT IN WHITE BEAN CROSSES (PHASEOLUS VULGARIS L.)

Mirjana VASIĆ, Jelica GVOZDANOVIĆ-VARGA and Dušanka BUGARSKI

Institute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia

Abstract

Vasić M., J. Gvozdanović-Varga and D. Bugarski (1996): Seed yield per plant in white bean crosses (Phaseolus vulgaris I..). - Genetika. Vol. 28, No. 3, 171-176.

Five parent populations (two female lines chosen from the populations Gradištanac and Tetovac and the varieties Medijana, Biser and Oplenac as male components) and six populations of each F₁ and F₂ generations were analyzed for seed yield per bean plant. Considering the parents, the highest seed yield was achieved with Tetovac, then Gradištanac and the lowest with Oplenac. Seed yield variability per plant was median to high. Heterotic effect or dominance of the parent with higher mean value occurred as an inheritance mode in the F₁generation. Heterosis was highest in the cross between Gradištanac x Oplenac. Mean values of the populations was reduced in the F₂generation. Inheritance mode for seed yield per plant was intermediary in all studied crossing combinations. Heritability of seed yield per plant was high in most combinations. Considering the studied characters, plants with transgressions occurred in the crosses with Gradištanac.

Key words: bean (Phaseolus vulgaris L.), genetic analysis, yield of seeds/plant.

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 177-183

Serbia

UDC 575:596.773.4

Original scientific paper

INTRASPECIFIC VARIATION IN THE NUMBER OF MALE SEX COMB TEETH IN DROSOPHILA BIPECTINATA

Rakhee BANERJEE and B.N. SINGH

Genetics Laboratory, Department of Zoology

Banaras Hindu University, Varanasi - 221 005, India

Abstract

Banerjee R., and B.N. Singh (1996): Intraspecific variation in the number of male sex comb teeth in Drosophila bipectinata. - Genetika. Vol. 28, No. 3, 177-183.

The number of sex comb teeth was studied in nine mass culture stocks of Drosophia bipectinata established from naturally impregnated females collected from ecogeographically different localities in India during 1993-95 and one laboratory strain (origin Nepal) maintained since 1988 in the laboratory. The mean number of sex comb teeth varied from 13.58 to 14.54 indifferent geographic stocks. There was much individual variation (minimum 10 teeth and maximum 18 teeth) in the number of sex comb teeth. Statistically significant differences in the mean number of teeth were indicated among different stocks by ANOVA which provides evidence that there is genetic heterogenity with respect to the number of sex-comb teeth in populations of D. bipectinata.

Key words: Drosophila bipectinata, laboratory stocks, sex comb teeth number, intraspecific variation

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 185-192

Serbia

UDC 575.612.112

Original scientific paper

GENETIC DETERMINATION OF SISTER CHROMATID EXCHANGE (SCE) VARIATION IN NONSTIMULATED HUMAN LYMPHOCYTES AFTER TREATMENT WITH CYTOSTATICS IN VITRO

Olivera MILOŠEVIĆ-DJORDJEVIĆ¹ , Dragoslav MARINKOVIĆ², and Slobodan ARSENIJEVIĆ³

¹Faculty of Science, University of Kragujevac, 34000 Kragujevac

²Faculty of Science, University of Belgrade, 11000 Belgrade

³Clinical Hospital of Kragujevac, 34000 Kragujevac, Yugoslavia

Abstract

Milošević-Djordjević O., D. Marinković and S. Arsenijević (1996): Genetic determination of sister chromatid exchange (SCE) variation in nonstimulated human lymphocytes after treatment with cytostatics in vitro. - Genetika, Vol. 28, No. 3, 185 - 192.

In this experiment the lymphocytes of heparinized blood from healthy donors were taken and exposed to treatment with cytozar, 5-fluorouracil and mefosfamide. The sister chromatid exchange (SCE) test was applied, which showed the extent of within-individual and between-individual variation in mutagenically treated and non-treated cells. Among cells treated with cytozar or 5-fluorouracil, a mild but non-significant increase of SCEs could be observed, whereas the cells treated with mafosfamide had a significantly higher frequency of SCEs. Both between and within-individual variation was somewhat higher than among cells without mutagenic treatment.

Key words: sister chromatide, lymphocytes, cytostatics

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Published in „GENETIKA“ Vol. 28, No.3 (1996), pp. 193-199

Serbia

UDC 575:596.773.4

Original scientific paper

THE MECHANISMS OF SEXUAL SELECTION OF Drosophila melanogaster

Snežana STANIĆ

Faculty of Science, Kragujevac, Yugoslavia

Abstract

Stanić S. (1996): The mechanisms of sexual selection of Drosophila melanogaster. - Genetika, Vol. 28, No. 3, 193 - 199.

Two groups of Drosophila melanogaster (group S - maturation in biological surroundings, group WS - maturation under conditions of absolute isolation) were tested for some parameters of reproductive success (mating frequency, type of mating and the time until the beginning of mating) under conditions of multiple choices. Morphometric analysis of the individuals was also carried out, length of the wings being the indicator of their size. The significance of the differences in values obtained for the stated characteristics was ascertained by non parametric (X², t-test) tests and by the parametric Mann-Whitney U test. The results show a significant correlation between the sizes of mated males and females of the tested species indicating that their crossing is assortative. There was also non-random mating of Drosophila melanogaster individuals taken from the same conditions of maturation.

Key words: Drosophila melanogaster, reproductive success, males, females, mating

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