GENETIKA, Vol. 29, No.3 (1997)
Jadranka IVEZIĆ, Srbislav DENČIĆ, Marija KRALJEVIĆ-BALALIĆ, Ljiljana MERKULOV and Dragana VUJIČIĆ
AN EVALUATION OF SPIKE CHARACTERIST1CS IN TETRAPLOD AND HIEXAPLOD WHEAT GENOTYPES [Abstract]
Goran TODOROVIĆ, Gordana ŠURLAN-MOMIROVIĆ, Ilija ŠATARIĆ, Tomislav ŽIVANOVIĆ and Slaven PRODANOVIĆ
GENETIC ANALYSIS AND HETEROS1S OF YIELD IN MAIZE HYBRIDS [Abstract]
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Milan DAMJANOVIĆ, Milan ZDRAVKOVIĆ and Tatjana SRETENOVIĆ-RAJIČIĆ
EPISTATIC GENE EFFECTS ON INHERITANCE OF FRUIT WEIGHT PER PLANT IN TOMATO (LYCOPERSICUM ESCULENTUM MILL.) [Abstract]
Yueming VAN, Slaven PRODANOVIĆ, Gordana ŠURLAN-MOMIROVIĆ, Dragica ZORIĆ and Guangtian LIU
CHROMOSOMAL LOCATION OF GENES CODING WHEAT GLIAD1N PROTEINS BY HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS ANALYSIS [Abstract]
Veselinka ZEČEVIĆ and Desimir KNEŽEVlĆ
VARIABILITY AND COMPONENTS OF VARIANCE FOR HARVEST INDEX IN WHEAT (TRITICUM AESTIVUM L.) [Abstract]
Danijela PETRONIJEVIĆ, Marija GUČ-ŠĆEKIĆ,Tanja LALIĆ, Marina ĐURlŠlĆ, Jovan SAVIĆ and Predrag MINIĆ
CYSTIC FIBROSIS: OUR EXPERIENCE IN SCREENING OF CFTR MUTATIONS [Abstract]
Ravi PARKASH, Dev KARAN and Ashok K. MUNJAL
PHENOTYPIC VARIABILITY IN INDIAN DROSOPHILA SPECIES POPULATIONS: I. QUANTITATIVE TRAITS [Abstract]
Dev KARAN. Ashok K. MUNJAL and Ravi PARKASH
PHENOTYPIC VARIABILITY IN INDIAN DROSOPHILA SPECIES POPULATIONS: II. PIGMENTATION POLYMORPHISM [Abstract]
Mladen VUJOŠEVIĆ, Saša MALKOV, and Aleksandar JOVANOVIĆ
COMPUTERIZED CHROMOSOME COMPARISON – HOW MUCH DIFFERENCE IN HOMOLOGY OF TWO CHROMOSOMES IS ACCEPTABLE [Abstract]
Nada BARJAKTAROVIĆ and Miroslav WURZBURGER
SISTER-CHROMATID EXCHANGES IN PATIENTS WITH ACROMEGALIA [Abstract]
Bratislava SIVČEV
MINIMAL LIST OF DESCRIPTORS AS A BASIS FOR RRANGING GRAPEVINE VARIETIES IN ECOLOGICAL-GEOGRAPHIC GROUPS [Abstract]
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 153-162
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 75:633.1
Original scientific paper
AN EVALUATION OF SPIKE CHARACTERIST1CS IN TETRAPLOD AND HIEXAPLOD WHEAT GENOTYPES
Jadranka IVEZIĆ, Srbisav DENČIĆ2, Marija KRALJEVIC-BALAL1Ć2,
Ljiljana MERKULOV and Dragana VUJIČIĆ1
1 Institute of Biology. 21000 Novi Sad,
2lnstitute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia
Abstract
Ivezić Jadranka, Srbisav Denčić, Marija Kraljević-Balalić, Ljiljana Merkulov and Dragana Vujičić (1997): An evaluation of spike, characteristics in tetraploid and hexaploid wheat genotypes - Genetika, Vol. 29, No. 3. 153-162.
Some quantitative characteristics of the spike such as: spike weight, weight and number of grains per spike and spike index were analyzed in eight tetraploid and eight hexaploid genotypes of wheat, during a three year experiment. The target of this research was to detect differences in the analyzed characteristics between genotypes of different ploidy level, as well as the variability of the characteristics with respect to the genotypes, years and year x genotype interactions. Our results showed that tetraploid genotypes had lower values for all analyzed characteristics. Significant differences between tetraploid and hexaploid genotypes were found for grain weight per spike and for spike index. Highly significant variability was found within the analyzed groups of wheat with respect to years, genotypes and year x genotype interactions for spike weight and number of grains per spike for both groups. The same conclusion holds for the grain weight per spike and the spike index for the tetraploid genotypes only.
Key words: wheat, tetraploids, hexaploids. quantitative characteristics of spike, variability
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 163-172
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.2:633.15
Original scientific paper
GENETIC ANALYSIS AND HETEROS1S OF YIELD IN
MAIZE HYBRIDS
Goran TODOROVIĆ1, Gordana ŠURLAN-MOMIROVIĆ2. Ilija ŠATARIĆ,1 Tomislav ŽIVANOVIĆ2 and Slaven PRODANOVIĆ2
1Maize Research Institute ,,Zemun Polje", 11080 Belgrade-Zemun
2Faculty of Agriculture, University of Belgrade, 11080 Belgrade. Yugoslavia
Abstract
Todorović G., G. Šurlan-Momirović. I. Šatarić, T. Živanović, and S. Prodanović (1997): Genetic analysis and heterosis of yield in maize hybrids. - Genetika, Vol. 29, No. 3, 163-172.
The inheritance patterns of grain yield were studied using the generation mean analysis method (MATHER, 1949; MATHER and JINKS, 1971, 1982). Two hyhrids with common parents were observed whereby 14 generations included in the genetic analysis were formed. Based on the results obtained the conclusion emerges that dominant variation primarily effected by the genetic basis of the material analyzed predominated in the genetic variation. Epistatic effects were less significant compared with dominant genes. However, they were more significant in relation to additive genes. In addition, two-gene epistasis was found to predominate in relation to complementary epistasis as far as grain yield inheritance in the hybrid is concerned. In the case of both hybrids low positive values of the two-gene epistasis between the additive genes were found to contribute to an insignificant increase of the additive gene effects. Highly significant values of heterosis were found for grain yield. The values for dominant genes x environment interaction were higher than the values for additive genes x environment interactions.
Key words: additive, dominant, genes, epistasis, heterosis, heritability, maize
Corresponding author:
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 173-180
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 635.64
Original scientific paper
EPISTATIC GENE EFFECTS ON INHERITANCE OF FRUIT WEIGHT PER PLANT IN TOMATO (LYCOPERSICUM ESCULENTUM MILL.)
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Milan DAMJANOVIĆ, Milan ZDRAVKOVIĆ and Tatjana SRETENOVIĆ-RAJIČIĆ
Institute for Agricultural Research ,,SRBIJA", Center for vegetable Crops, 11240 Smederevska Palanka, Yugoslavia
Abstract
Zdravković Jasmina, Ž. Marković. M. Damjanović, M. Zdravković and Tatjana Sretenović-Rajičić (1997): Epistatic gene effects on inheritance of fruit weight per plant in tomato (Lycopersicum esculentum Mill. - Genetika. Vol. 29, No. 3, 173-180.
Genetic analysis of six tomato hybrids obtained from 6 inbred lines (D-150, S-49. S-35.11-52. Kg-z and SP-109) was made. Their F1, F2, BC1 and BC2 generations were raised. Genetic effects were estimated by applying the additive - dominance model with three and six parameters, epistatic gene effects were estimated by applying the model with six parameters (MATHER and JINKS, 1982). Concerning the fruit weight per plant, there were significant differences between the mean values of the parents and their offspring. There was a considerable epistatic effect in five investigated hybrids (S-49 x S-35, S-49 x SP-109, S-35 x SP-109, H-52 x SP-109 and Kg-z x SP-109). The duplicate type of epistasis which had a negative effect on fruit weight was found in three hybrids (S-49 x S-35, H-52 x SP-109 and Kg-z x SP-109). Apart from type (1) epistasis (dominance x dominance) which was significant in 4 hybrids, type (j) epistasis was also prominent in three combinations.
Key words: tomato hybrids, epistatic gene effects, fruit weight
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 181-193
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 576.316:633.11
Original scientific paper
CHROMOSOMAL LOCATION OF GENES CODING WHEAT
GLIAD1N PROTEINS BY HIGH PERFORMANCE CAPILLARY
ELECTROPHORESIS ANALYSIS
Yueming VAN1, Slaven PRODANOVIĆ2, Gordana ŠURLAN-MOMIROVIĆ2, Dragica ZORIĆ2 and Guangtian LIU1
1College of Planl Science and Technology, China Agricultural University,
100094 Beijing, The People's Republic of China
2Faculty of Agriculture, University of Belgrade, 11080 Belgrade, Yugoslavia
Abstract
Yan Y., S. Prodanović, G. Šurlan-MomiroviĆ, D. ZoriĆ and G. Liu (1998): Chromosomal location of genes coding wheat gliadin proteins by high performance capillary elecimphoresis analysis. - Genetika, Vol. 29, No.3, 181-193.
Gliadin proteins of the hexaploid wheat variety ,,Chinese Spring" (CS) and its nullisomic-tetrasomic (NT) and ditelocentric (DT) aneuploid lines, were fractionated by a new, more powerful technique - High Performance Capillary Electrophoresis (HPCE). By using uncoated glass capillaries (47 cm long and 50 μm i.d.) in borate + SDS + ACN buffer system on a Beckman P/ACE 5500 system at 15 kV and 30°C, forty-five gliadin components were detected in CS. According to the presence, absence or amounts of gliadin peaks in NT and DT lines of CS, all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (Gli-1 and Gli-2 1oci). Of 45 components investigated, 27 were coded by the Gli-1 locus (5 by Gli-Al, 10 by Gli-Bl and 12 by Gli-Dl), and 18 were coded by the Gli-2 locus (7 by GK-A2, 7 by GH-B2 and 6 by GH-D2). Peaks 6 and 9 seemed to contain polypeptides controlled by loci on both 6A and 6B or 6B and 6D, respectively. Our results support HPCE as a powerful tool for qualitative and quantitative genetic studies of wheat storage proteins.
Key words: genetic control, gliadias, HPCE, wheat
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 195-202
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 633.11:631.524
Original scientific paper
VARIABILITY AND COMPONENTS OF VARIANCE FOR HARVEST INDEX IN WHEAT (TRITICUM AESTIVUM L.)
Veselinka ZEČEVIĆ and Desimir KNEŽEVlĆ
Agricultural Research Institute SERBIA, Center for Small Grains - Kragujevac, 3
4000 Kragujevac Yugoslavia
Abstract
Zečević V. and D. Knežević (1997): Variability and components of variance for harvest index in wheat (Triticutn aestivum L.). - Genetika. Vol. 29, No. 3, 195-202.
Variability, heritability and components of variance were studied in 50 cultivars from different selection centers of the world. Plants were grown in the experimental field in 3 replications during two vegetative seasons. Samples of 60 wheat plants (20 plants in 3 repetitions) were analyzed in full maturity stage. Harvest index was calculated as the ratio of grain yield and total aerial part of the plant. The highest harvest index was found in cv. Peking 8 (44.6%), and the lowest in cv. Jawa (23.8%). On average for all cultivars, the harvest index was similar in both years of investigation (35.4%; 35.2% resp.). Cullivars showed differences in variability of harvest index. The mean coefficient of variation for all cultivars and both years was V = 12.0%. The lowest coefficient of variation was found in cv. Peking 8 (V = 7.0%), and the highest cv Brock (V = 17.8%). High heritability (h2 = 75%) was established for this trait. Highly significant differences in mean values for harvest index between cultivars were shown by analysis of variance. Differences between year and the interaction cultivar/year were also highly significant. Components of phenotypic variance showed that genetic factors (53.7%) had the highest impact on expression of harvest index, while the interaction cultivar/year showed less impact (35%). Year did not contribute significantly to the variance.
Key words: wheat, cultivar, harvest index, heritability, variability, components of variance
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Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 203-208
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 577.21
Original scientific paper
CYSTIC FIBROSIS: OUR EXPERIENCE IN SCREENING OF CFTR
MUTATIONS
Danijela PETRONIJEVIĆ, Marija GUČ-ŠĆEKIĆ,Tanja LALIĆ, Marina ĐURIŠIĆ, Jovan SAVIĆ and Predrag MINIĆ
Mother and Child Health Institute of Serbia, Belgrade, Yugoslavia
Abstract
Petronijević D., M. Guč-Šćekić, T. Lalić, M. Đurišić, J. Savić and P. Minić (1997): Cystic fibrosis: Our experience in screening of CFTR mutations. - Genetika, Vol. 29, No. 3, 203-208.
To initiate the complete characterization of mutations in the CFTR gene in Yugoslav cystic fibrosis (CF) patients, we screened 82 patients for CF mutations using polyacrylamide and denaturing gradient gel electrophoresis (PAGE and DGGE). Three different mutations (ΔF508, G542X and 621 + 1G>T) accounted for 73, 78% of the CF alleles in Yugoslav patients, of which the ΔF508 mutation had a frequency of 69,37%. Further previously described mutations accounted for another 7, 32% CF alleles and one previously undescribed mutation (2723del1TT) was found in one chromosome. Thus, so far, we have identified mutations in the CFTR gene in Yugoslav CF patients accounting for 81, 1% of the CF alleles.
Key words: CFFR gene, cystic fibrosis, mutation
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 209-218
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 596.773.4:575.21
Original scientific paper
PHENOTYPIC VARIABILITY IN INDIAN DROSOPHILA SPECIES
POPULATIONS: I. QUANTITATIVE TRAITS
Ravi PARKASH, Dev KARAN and Ashok K. MUNJAL
Genetics Research Laboratory, Department of Biosciences, Maharshi Dayanand University, Rohtak - 124001, India
Abstract
Parkash R., D. Karan and A.K. Munjal (1997): Phenotypic variability in Indian Drosophila species population: I. Quantitive traits. - Genetika, Vol. 29, No. 3, 209-218.
Geographical variations in six quantitative morphological traits were studied in eight Indian natural populations of D, kikkawai. Four morphometrical traits (body weight, wing length, thorax length and ovariole number) demonstrated significant clinical variation with increase in latitude (r = > 0.99). The sternopleural and abdominal bristles showed no significant correlation with body weight or with latitude. South Indian populations had smaller mean values for body weight, wing and thorax lengths as well as ovariole number than the northern populations. Thus, such traits are differentiated among Indian geographical populations of D. kikkawai and are adaptively maintained by selective pressure from variable environmental conditions on the Indian subcontinent.
Key words: Morphometrical traits, latitudinal clines, Indian subcontinent, Drosophila kikkawai
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 219-229
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 596.773.4:575.21
Original scientific paper
PHENOTYPIC VARIABILITY IN INDIAN DROSOPHILA SPECIES POPULATIONS: II. PIGMENTATION POLYMORPHISM
Dev KARAN. Ashok K. MUNJAL and Ravi PARKASH
Genetics Research Laboratory, Department of Biosciences, Maharshi Dayanand University, Rohtak - 124001, India
Abstract
Karan D., A.K. Munajl and R. Parkash (1997): Phenotypic variability in Indian Drosophila species populations: II. Pigmentation polymorphism. - Genetika, Vol. 29, No. 3, 219-229.
Fourteen natural populations of D. melanogaster collected along diverse latitudinal and altitudinal ranges, were analysed for thoracic trident pigmentation at two different tempertures (17°C and 25°C) for both the sexes.Statistical analysis in dicated significant intra- and inter-populational variation. Clinal patterns for thoracide trident pigmentation along latitude as well as altitude were significantly correlated with temperture. Crosses between dark (Shimla) and light (Kalka) variants of trident pigmentation produced intermediate flies, and a clear maternal effect was observed in the reciprocal F1 flies. This is the first report on thoracic trident pigmentation analysis in Indian natural populations of D. melanogaster suggesting that phenotypic plasticity due to temperature differences has resulted in geographic differentiation.
Key words: thoracic pigmentation, latitudinal and altitudinal cline, Indian populations, Drosophila melanogaster
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 231-239
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 576.316
Original scientific paper
COMPUTERIZED CHROMOSOME COMPARISON – HOW MUCH DIFFERENCE IN HOMOLOGY OF TWO CHROMOSOMES IS ACCEPTABLE
Mladen VUJOŠEVIĆ1, Saša MALKOV2, and Aleksandar JOVANOVIĆ2
Department of Genetics, Institute for Biological Research
“Siniša Stanković",11060 Belgrade
and
School of Mathematics, 11000 Belgrade, Yugoslavia
Abstract
Vujošević M., S. Malkov and A. Jovanović (1997): Computerized chromosome comparison - how much difference in homology of two chromosomes in acceptable - Genetika, Vol. 29, No. 3, 231-239.
Mathematical representations of images of G-banded chromosome No 1 from the wood mouse, Apodemus flavicollis, were used as a model for a computerized chromosome comparison. Our aim was to explore the possibility of establishing a limit for deciding whether two chromosomes are the same or not. A total of 14189 comparisons between No 1 chromosomes from the same and different animals indicated that the limit was 20% for the same chromosome, and 25% for different chromosomes. The latter limit was established by comparing chromosome No 1 with different chromosomes from the same karyorype of similar size or having a similar number of banks. The results showed that the developed mathematical method could be used with great confidence for comparison and identification of G-banded chromosomes regardless of the degree of their contraction.
Key words: wood mouse, chromosome comparison, mathematical method
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 241-243
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 576.316
Short communication
SISTER-CHROMATID EXCHANGES IN PATIENTS WITH ACROMEGALIA
Nada BARJAKTAROVIĆ and Miroslav WURZBURGER
Zvezdara University Medical Center, 11000 Belgrade, Yugoslavia
Abstract
It is already known that sister-chromatid exchanges (SCE) are a sensitive indicator of DNA damage. Therefore, it was of interest to determine whether there is any connection between certain hormonal disbalances and changes in DNA level.
Investigations in our laboratory have already shown, for example, that, although the frequency of structural chromosomal aberrations does not increase in newborns after progesterone treated pregnancies, there is a statistically significant rise in the number of observed SCE-s compared to the control group (LUKIĆ and BARJAKTAROVIĆ, 1987).
This time we selected a group of 18 patients with acromegalia, in different stages of the disease. cytogenetic analyses were performed on peripheral blood lymphocytes, and differential harlequin staining carried out using the FPG technique (WOLFF and PERRY, 1974), with BUdR at 5 μ ml-1. We analysed 100 second division cells for the presence of SCE-s in each patient. Our laboratory control for SCE-s in the adult population is 8.02±0.43 obtained from 1000 second division cells. Patients with acromegalia showed widely different mean values of SCE/cell starting with 7.3 up to 13.6, the overall mean value being 10.9±3.6. Although the dispersion was considerable, an obvious statistically significant increase compared to the control was found (P < 0.05).
To verify whether growth hormone disturbances are the main cause of this rise in the number of SCE-s, CRESCORMONE was tested in a system in vitro as well. Beside the control, five concentrations of CRESCOKMONE in the presence of BUdR were set up (0.4. 1, 2, 20 and 200/pg/ml of culture). Again, almost a linear fit between the number ol SCE-s and different concentrations of this drug in the culture was obtained.
Although further work is needed to confirm this finding both in in vivo and in vitro systems, we considered that we should draw attention to this phenomenon.
Published in „GENETIKA“ Vol. 29, No.3 (1997), pp. 245-259
© 1997 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 634.84.85
Original scientific paper
MINIMAL LIST OF DESCRIPTORS AS A BASIS FOR ARRANGING GRAPEVINE VARIETIES IN ECOLOGICAL-GEOGRAPHIC GROUPS
Bratislava SIVČEV
"Radmilovac" Experimental School Estate Faculty of Agriculture, University of Belgrade, 11000 Belgrade, Yugoslavia
Abstract
Sivčev B. (1997): Minimal list of descriptors as a basis for arranging grapevine varieties in ecological - geographic groups. - Genetika. Vol. 29, No. 3, 245-259.
On the basis of 22 morphological characteristics, seventy-two grapevine varieties intended for white wine production were arranged into three ecological-geographic groups, with two subgroups in each. The accuracy achieved was 80.56%. The starling basis for arranging varieties from the groups convarietas occidentalis Negr., subconvarietas iberica Nem., convarietus pontica Negr., subconvarietas georgica Negr. and convarietas orientalis Negr., subconvarietas anthasiastica Negr. was confirmed by discriminant analysis. The accuracy varied from 89.5% for the varieties convarietas occidentalis Negr., subconvarietas galica Nem., to 73.0% for convarietas pontica Negr. and subconvarietas balcanicai Negr., and to 50% for convarietas orientalis Negr. and subconvaielas caspica Negr.
Key words: variety, minimal list of descriptors, ampelography, morphology, ecological-geographic groups