GENETIKA, Vol

GENETIKA, Vol. 31, No. 2 (1999)

Mirjana IVANČEVIĆ, Tatjana SRETENOVIĆ RAJIČIĆ, Nenad PAVLOVIĆ, and

Jasmina ZDRAVKOVIĆ

CORRELATION ANALYSIS OF SOME QUANTITATIVE TRAITS OF AUTOCHTONOUS CABBAGE POPULATIONS (BRASSICA OLERACEA VAR. CAPITATA L.) [Abstract]

Bogoljub ZEČEVIĆ, Dušan STEVANOVIĆ, Slaven PRODANOVIĆ, and Radiša ĐORĐEVIĆ

INHERITANCE OF YIELD COMPONENTS IN DIALLEL CROSSING OF DIVERGENT GENOTYPES OF PEPPER (CAPSICUM ANNUUM L.) [Abstract]

Dejan CVIKIĆ, Jasmina ZDRAVKOVIĆ, Gordana ŠURLAN-MOMIROVIĆ, Zoran SUŠIĆ, and Radiša ĐORĐEVIĆ

INHERITANCE MODE AND PHENOTYPIC VARIABILITY FOR FRUIT FIRMNESS IN NOR AND RIN TOMATO GENOTYPES [Abstract]

Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, and Mirjana MIJATOVIĆ

INHERITANCE MODE OF FRUIT SHAPE IN TOMATO [Abstract]

Radiša ĐORĐEVIĆ, Milan ZDRAVKOVIĆ. Bogoljub ZEČEVIĆ, and Tatjana SRETENOVIĆ RAJIČIČ

AFILA GENE EFFECTS ON ABSOLUTE SEED MASS IN PEAS (PISUM SATIVUM L.) [Abstract]

Danijela RADIVOJEVIĆ, Marija GUĆ-ŠĆEKIĆ, Emmanuel KANAVAKIS, Jovan SAVIĆ, Maria TZETIS, Thalia ANTONIADI, Predrag MINIĆ, Marina ĐURIŠIĆ, Tanja LALIĆ, and Aleksandar CVETKOVIĆ

IDENTIFICATION OF NON-ΔF508 MUTATIONS IN THE CFTR GENE USING DENATURING GRADIENT GEL ELECTROPHORESIS [Abstract]

Živoslav MARKOVIĆ, Jasmina ZDRAVKOVIĆ, and Milan DAMJANOVIĆ

LOCAL POPULATIONS AND SEMI-WILD TOMATO FORMS AS A SOURCE OF GENETIC DIVERGENCE [Abstract]

Milena STEVANOVIĆ and Marija GUĆ-ŠĆEKIĆ

APPLICATION OF MOLECULAR TECHNIQUES IN DIAGNOSTICS OF CHROMOSOMAL DISORDERS [Abstract]

Tanja LALIĆ, Marija GUĆ-ŠĆEKIĆ, Marina ĐURIŠIĆ, Danijela RADIVOJEVIĆ, Dragan ZAMUROVIĆ, and Slobodanka TODOROVIĆ

GENETIC LINKAGE FOR CARRIER IDENTIFICATION AND PRENATAL DIAGNOSIS OF DUCHENNE/BECKER MUSCULAR DYSTROPHY [Abstract]

Stanislav ŠESEK and Ankica KONDIĆ

HERBICIDE TOLERANCE OF WHEAT GENOTYPES IN IN VITRO CULTURE [Abstract]

Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 91-96

Serbia

UDC 575.1; 635.34

Original scientific paper

CORRELATION ANALYSIS OF SOME QUANTITATIVE TRAITS OF AUTOCHTONOUS CABBAGE POPULATIONS (BRASSICA OLERACEA

VAR. CAPITATA L.)

Mirjana IVANČEVIĆ, Tatjana SRETENOVIĆ RAJIČIĆ, Nenad PAVLOVIĆ, and

Jasmina ZDRAVKOVIĆ

Agricultural Research Institute "Serbia", Center for Vegetable Crops, 11420

Smederevska Palanka, Yugoslavia

Abstract

Ivančević M., T. Sretenović Rajičić, N. Pavlović, and J. Zdravković (1999): Correlation analysis of some quantitative traits of autochthonous cabbage populations (Brassica oleracea var. capitata L.). — Genetika, Vol. 31, No. 2, 91-96.

In order to determine the initial material for breeding aimed at obtaining favorable agronomic traits, we investigated nine local populations and one cabbage variety. The trial was set up on an experimental plot of the Center for Vegetable Crops in Smederevska Palanka by applying the method of random block system in five replications during the years 1997 and 1998. Interdependence of the traits (rosette diameter, number of leaves per rosette, plant mass, head diameter, head height, length of the interior core, length of the exterior core, and head mass) was determined by coefficient of correlation (HADŽIVUKOVIĆ, 1991). The coefficients of correlation demonstrated a high positive correlation ratio between head mass - plant mass (r = 0.769). The lowest positive correlation ratio was found for rosette diameter - core length (r = 0.142). The number of leaves per rosette was in negative correlation with the head diameter (r = - 0.309) and the head height (r = - 0.280)

Key words: cabbage, population, trait, coefficient of correlation, head mass

Corresponding author: Mirjana Ivančević, Agricultural Research Institute "Serbia", Center for Vegetable Crops, Karađorđeva 71, 11420 Smederevska Palanka, Yugoslavia, tel.: 381-26-323170; fax: 381-26-323785; e-mail: cfvcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 97-105

Serbia

UDC 575.1; 635.64

Original scientific paper

INHERITANCE OF YIELD COMPONENTS IN DIALLEL CROSSING OF DIVERGENT GENOTYPES OF PEPPER (CAPSICUM ANNUUM L.)

Bogoljub ZEČEVIĆ¹, Dušan STEVANOVIĆ¹, Slaven PRODANOVIĆ², and Radiša ĐORĐEVIĆ¹

¹ Center for Vegetable Crops, 11420 Smederevska Palanka,

² Faculty of Agriculture, 11000 Beograd, Yugoslavia

Abstract

Zečević B., D. Stevanović, S. Prodanović, and R. Đorđević (1999): Inheritance of yield components in diallel crossing of divergent genotypes of pepper (Capsicum annuum L.). - Genetika, Vol. 31. No. 2, 97-105.

On the basis diallel crossing (without reciprocal) of six divergent genotypes of pepper (Palanačka babura, Mačvanka, Palanačka kapija, Romana, Kobra, and Feferona žuta ljuta), the mode of inheritance and the components of genetic variance were analyzed for fruit weight and number of fruits per plant. The investigation was based on data of F₁ generation. In the most of hybrids, the partial dominance was estimated as the mode of inheritance for both traits. The analysis of components of genetic variance showed that the main part of genetic variance belonged to the additive gene effect for yield components. The high values of heritability indicated also more important role of additive genes. On the basis of the calculated results it can be concluded that in the breeding of pepper for yield, genotypes with high average values for fruit weight and number of fruits per plant should be selected.

Key words: paper, Capsicum annuum, diallel, inheritance

Corresponding author: Bogoljub Zečević, Agricultural Research Institute "Serbia". Center for Vegetable Crops, Karađordeva 71, 11420 Smederevska Palanka, Yugoslavia, tel.: 381-26-323170; fax: 381-26-323785; e-mail: cfvcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 107-114

Serbia

UDC 575.118: 635.64

Original scientific paper

INHERITANCE MODE AND PHENOTYPIC VARIABILITY FOR FRUIT

FIRMNESS IN NOR AND RIN TOMATO GENOTYPES

Dejan CVIKIĆ¹, Jasmina ZDRAVKOVIĆ¹, Gordana ŠURLAN-MOMIROVIĆ²,

Zoran SUŠIĆ¹, and Radiša ĐORĐEVIĆ¹

¹ Institute for Agricultural Research "SRBIJA", Center for Vegetable Crops,

11240 Smederevska Palanka,

² Faculty of Agriculture, 11000 Beograd, Yugoslavia

Abstract

Cvikić D., J. Zdravković, G. Šurlan-Momirović, Z. Sušić, and R. Đorđević (1999): Inheritance mode and phenotypic variability for fruit firmness in nor and rin tomato genotypes. - Genetika. Vol. 31, No. 2, 107-114.

By using the method of diallel (without reciprocal crosses), we carried out the crossing of two tomato lines with normal ripening and four mutant lines characterized by postponed shelf life (nor and rin). The genetic analysis was made both of parental lines and their descendants, fi and F₂ generations. The mode of inheritance for fruit firmness was estimated by using the significance test of mean values of F₁, and F₂ generations as compared to the mean values of their parents. The segregation of the genetic variance was performed by applying the mather and jinks method (1971), while their combining abilities were analyzed by the griffing method (1956), method 2, mathematical model 1. As for the inheritance mode of the fruit firmness feature in tomato, both investigated generations showed the following modes of inheritance: intermediary inheritance mode, partial dominance of parents with the soft and the firm fruit, dominance of parents with the soft and the firm fruit, as well as superdominance of parents with the firm fruit. The additive variance prevailed over the dominance one. The highest heterosis for the feature of fruit firmness was found in the hybrid NR-2 x NR-12 (18.93%). Three parental lines (NR-3, NR-11 and NR-12) were characterized by significant GCA values in F₁ and F₂ generations, while significant SCA values were found in the two F₁hybrids (NR-2 x NR-12 and NR-11 x NR-12). The mutant tomato lines (NR-3, NR-11 and NR-12) were characterized by greater firmness as compared to the normal lines, and possessing such features, they should be included in the selection programs. The main reason for this is the fact that these programs are aimed at creating commercial F₁ hybrids with firm fruits.

Key words: tomato, fruit firmness, nor and rln, diallel crosses, inheritance mode

Corresponding author: Dejan Cvikić, Agricultural Research Institute "Serbia". Center for Vegetable Crops, Karađorđeva 71,11420 Smederevska Palanka, Yugoslavia, tel.: 381-26-323170; fax: 381-26-323785; e-mail: cfvcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 115-123

Serbia

UDC 575.118, 635.64

Original scientific paper

INHERITANCE MODE OF FRUIT SHAPE IN TOMATO

Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, and Mirjana MIJATOVIĆ

Institute for Agricultural Research "SRBIJA", Center for Vegetable Crops,

11240 Smederevska Palanka, Yugoslavia

Abstract

Zdravković J., Ž. Marković, and M. Mijatović (1999): Inheritance mode of fruit shape in tomato. Genetika, Vol. 31, No. 2, 115-123.

By diallel crossings of 6 parental genotypes (D-150, S-49, S-35 and H-52, Kg-ž and SP-109) and their descendants obtained from these crossings (F_l, F₂, BC₁ and BC₂), we investigated the ratio between the horizontal and vertical diameter of tomato fruit which was represented by the index, i.e. tomato fruit shape. The inheritance mode of fruit shape in tomato was determined by the mean generation analysis (MATHER and JINKS, 1982). The additive gene effects were the most significant in inheriting the fruit shape. Most frequently expressed effects were additive x dominance interaction, followed by additive x additive gene effects. The duplicate type of epistasis was relatively stable.

Key words: tomato, inheritance mode, gene effects, fruit shape

Corresponding author: Jasmina Zdravković, Institute for Agricultural Research "Serbia", Center for Vegetable Crops. Karađorđeva 71, 11420 Smederevska Palanka. Yugoslavia, tel.: 381-26-323170; fax: 381-26-323785; e-mail: ciVcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 125-132

Serbia

UDC 575.113.2; 635.656

Original scientific paper

AFILA GENE EFFECTS ON ABSOLUTE SEED MASS IN PEAS (P1SUM

SATIVUM L.)

Radiša ĐORĐEVIĆ, Milan ZDRAVKOVIĆ. Bogoljub ZEČEVIĆ, and Tatjana

SRETENOV1Ć RAJIČIČ

Agricultural Research Institute "Serbia", Center for Vegetable Crops, 11420

Smederevska Palanka, Yugoslavia

Abstract

Đorđević R., M. Zdravković, B. Zečević, and T. Sretenović Rajičić (1999): "Afila" gene effects on absolute seed mass in peas (Pisum sativum L). - Genetika, Vol.31, No. 2, 125-132.

The af gene, at its recessive homozygous stage, causes the modification of the pea leaves so that they only form vines with stipules instead of normal structure leaves. In order to investigate the effect of the "afila" genotype on the absolute seed mass of the descendants, the pea variety Filigreen of the afila type was crossed with 12 other varieties possessing normal leaf structure. The genetic analysis of F₁ and F₂ generations (the segregation ratio between the normal and afila leaf type being 3:1) proved the monogenic character of this feature. The absolute seed mass in F₁ generation, which was characterized by normal leaf structure, was higher in the parents of both pea types. In F₂ generation that possessed normal leaf structure, the absolute seed mass was 2% higher on the average than the one in parents, while it was 6% lower as compared to the F₁ generation. The average absolute seed mass in afila pea plants was 5% lower than it was in their parents, and when compared to the F₁ generation, the absolute seed mass was 12% lower. The decrease in the absolute seed mass in F₂ generation of the afila genotype was conditioned by the reduction of the assimilation surface of the plant.

Key words: pea, a/gene, absolute seed mass

Corresponding author: Radiša Đorđević, Agricultural Research Institute "Serbia", Center for Vegetable Crops, Karađorđeva 71,11420 Smederevska Palanka, Yugoslavia, tel.: 381-26-323170; fax: 381-26-323785; e-mail: cfvcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 133-139

Serbia

UDC 575.224.2

Original scientific paper

IDENTIFICATION OF NON-ΔF508 MUTATIONS IN THE CFTR GENE USING DENATURING GRADIENT GEL ELECTROPHORESIS

Danijela RADIVOJEVIĆ¹, Marija GUĆ-ŠĆEKIĆ¹, Emmanuel KANAVAKIS²,

Jovan SAVIĆ', Maria TZETIS², Thalia ANTONIADI², Predrag MINIĆ¹, Marina

ĐURIŠIĆ¹, Tanja LALIĆ¹, and Aleksandar CVETKOVIĆ¹

'Mother and Child Health Institute, 11070 Belgrade, Yugoslavia,

²First Department of Pediatrics and Choremio Research Laboratory Unit

Molecular Medicine, St. Sophia's Children's Hospital, Athens, Greece

Abstract

Radivojević D., M. Guć-Šćekić, E. Kanavakis, J. Savić, M. Tzetis, T. Antoniadi, P. Minić, M. Burisić, T. Lalić, and A. Cvetković (1999): Identification of non-ΔF508 mutations in the CFTR gene using denaturing gradient gel electrophoresis. — Genetika, Vol. 3 1, No. 2, 133-139.

Cystic fibrosis (CF) is the most frequent lethal genetic disease of childhood. The disease is caused by more then 800 different mutations in the CFTR gene, with ΔF508 being the most frequent. In this study, denaturing gradient gel electrophoresis (DGGE) was used for a rapid analysis of the CFTR gene for the presence of non-ΔF508 mutations. In order to determine complete genotypes, we screened 22 CF patients with ΔF508 / non-ΔF508 genotypes (16 patients - 72.7%) and non-ΔF508 / non-ΔF508 genotypes (6 patients - 27.3%). In a sample of 28 non-ΔF508 CF alleles, 15 different CFTR mutations were identified: 621 + IG>T (13.6%); G542X (11.4%), S466X (6.8%), R1070Q (4.5%), 2789+5OA (4.5%), R334W (2.3%), E585X (2.3%), 2184insA (2.3%), 2907delTT (2.3%), 2723delTT (2.3%), 457TAT>G (2.3%), AI507 (2.3%), 582insG (2.3%), 1525-1G>A (2.3%) and R75X (2.3%). One of them, 2723delTT was a new frameshift mutation, found for the first time in one Yugoslav CF patient. Thus, authors demonstrated that DGGE was a method that permitted the rapid and efficient analysis of the CFTR gene regions, being particularly useful for genetic diagnosis.

Key words: cystic fibrosis, CFTR mutations, DGGE analysis

Corresponding author: Danijela Radivojević, Mother and Child Health Institute, Radoja Dakića 6-8, 11070 Belgrade, Yugoslavia

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 141-149

Serbia

UDC 575.22; 635.64

Original scientific paper

LOCAL POPULATIONS AND SEMI-WILD TOMATO FORMS AS A

SOURCE OF GENETIC DIVERGENCE

Živoslav MARKOVIĆ, Jasmina ZDRAVKOVIĆ, and Milan DAMJANOVIĆ

Agricultural Research Institute "Serbia", Center for Vegetable Crops, 11420

Smederevska Palanka, Yugoslavia

Abstract

Marković Ž, J. Zdravković, and M. Damjanović (1999): Local populations and semi-wild tomato forms as a source of genetic divergence. -Genetika, Vol. 31, No. 3, 141-149.

We investigated the sample of 25 entirely different local populations and semi-wild tomato forms originating from the area of the former Yugoslavia. We tested both the plant and the fruit characteristics: plant height, internode length, number of flowers up to the first inflorescence, earliness, fruit mass, number of locules per fruit, fruit shape, fruit color, jointless gene, fruit firmness, radial and concentrical cracks, green back, fruit fasciation, blossom-end rot, dry matter content, β-carotene content, C- vitamin content, tolerance to diseases and pests, tolerance to high and low temperatures, length of shelf-life of biologically mature tomato fruits, etc. The results showed that there was a strong genetic divergence for most of the investigated features, as well as the presence of the specific genes for a great number of traits favorable for selecting (i.e. earliness, fruit firmness, absence of cracks and fasciation, high content of vitamins, genetic resistance to diseases, pests and unfavorable weather conditions). This material could be highly significant and it could also be successfully used for the process of tomato selection.

Key words: tomato, local populations, genetic divergence

Corresponding author: Živoslav Marković, Agricultural Research Institute "Serbia", Belgrade, Center for Vegetable Crops, 11420 Smederevska Palanka, Yugoslavia e-mail: cfvcsp@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 151-161

Serbia

UDC 576.316

Original scientific paper

APPLICATION OF MOLECULAR TECHNIQUES IN DIAGNOSTICS OF CHROMOSOMAL DISORDERS

Milena STEVANOVIĆ¹ and Marija GUĆ-ŠĆEKIĆ²

¹ Institute of Molecular Genetics and Genetic Engineering, 11000 Belgrade

²Mother and Child Health Care Institute of Republic of Serbia, 11070 New

Belgrade, Yugoslavia

Abstract

Stevanović M. and M. Guć-Šćekić (1999): Application of molecular techniques in diagnostics of chromosomal disorders. - Genetika, Vol. 31, No. 2, 151-161.

Chromosomal disorders include all conditions associated with visible changes of the chromosomes. Chromosomal changes involve structural and numerical aberrations of autosome or sex chromosomes. Approximately 20% of all conceptions have chromosomal aberration, but most of them are spontaneously aborted and frequency of chromosomes aberrations at birth is 0.6%. Chromosomal abnormalities are not always associated with a disease. In general, autosomal abnormalities tend to be more severe than sex chromosome abnormalities, and they usually result in mental handicap and multiple congenital malformations. Therefore precise and reliable prenatal and postnatal diagnostics of chromosomal aberrations is of great importance for planning and obtaining health individuals. In the last few years, the fast progress has been made in more accurate diagnostics of chromosome aberration by application of new molecular techniques. The applications and advantages of the new molecular techniques, such as FISH, CGH and PCR are presented and discussed here. Therefore, fast, precise, accurate and reliable diagnosis of chromosome aberrations is possible only by the combination of cytogenetics and molecular techniques.

Corresponding author: Milena Stevanović, Institute of Molecular Genetic and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 794, 11001 Belgrade, Yugoslavia; tel.: 381-11-491391; fax: 381-11-492397; e-mail: stevanov@eunet.yu

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 163-170

Serbia

UDC 616.007.21:575.1

Original scientific paper

GENETIC LINKAGE FOR CARRIER IDENTIFICATION AND

PRENATAL DIAGNOSIS OF DUCHENNE/BECKER MUSCULAR

DYSTROPHY

Tanja LALIĆ¹, Marija GUĆ-ŠĆEKIĆ¹, Marina ĐURIŠIĆ¹, Danijela

RADIVOJEVIĆ¹, Dragan ZAMUROVIĆ¹, and Slobodanka TODOROVIĆ²

¹Mother and Child Health Institute of Serbia, 11070 Belgrade

² Institute of Neurology and Psychiatry for Children and Youth, 11000 Belgrade

Abstract

Lalić T., M. Guć-Šćekić, M Đurišić, D. Radivojević, D. Zamurović and S. Todorović (1999): Genetic linkage for carrier identification and prenatal diagnosis of Duchenne/Becker muscular dystrophy. — Genetika, Vol. 31, No. 2, 163-170.

In order to perform a complete postnatal and prenatal diagnosis of Duchenne/Becker (DMD/BMD) muscular dystrophy, 38 individuals in 11 families at risk were studied (15 affected boys). Multiplex PCR assay for the simultaneous amplification of 18 exons in the dystrophin gene was used for a deletion detection. Deletions were found in 11 of 15 cases (73.3%). In 4 cases where the deletion was not detected, linkage analyses were performed in order to determine the inheritance of DMD/BMD allele using 8 intragenic markers. In all of these cases it was possible to define the affected haplotype, which enabled the accurate and precise prenatal diagnosis in each subsequent pregnancy.

Key words: DMD/BMD, carrier status, deletions, prenatal diagnosis

Corresponding author: Tanja Lalić, Mother and Child Health Institute of Serbia, 11070 Belgrade, Yugoslavia

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Published in „GENETIKA“ Vol. 31, No.2 (1999), pp. 171-177

Serbia

UDC 632.954: 633.11

Original scientific paper

HERBICIDE TOLERANCE OF WHEAT GENOTYPES IN IN VITRO

CULTURE

Stanislav ŠESEK and Ankica KONDIĆ

Institute of Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia

Abstract

Šesek S. and Ankica Kondić (1999): Herbicide tolerance of wheat genotypes in in vitro culture. - Genetika, Vol. 31, No. 2, 171-177.

Tolerance of winter wheat (Triticum aestivum L.) genotypes to sulfonylurea, the active ingredient of a selective herbicide, was studied using in vitro mature embryo culture. The tolerance was tested on a modified MS (murashige and skoog, 1962) nutrient medium, which was enriched with three different concentrations of sulfonylurea (I-18.75 rngl^-1, II-37.50 mgl^-1, III-56.25 mgl^-1). Four cultivars, Pesma, Stepa, Renesansa and Proteinka, were used for isolation. During three months of cultivation, the survival of calli and regenerants were observed. After that, callus fresh weight and dry matter content were measured. The results showed that calli of all cultivars expressed a satisfactory level of tolerance to sulfonylurea, since in all of the treatments they had more than 50% of survived calli. In the treatment with the highest sulfonylurea concentration (III-56.25 mgl^-1) cv. Pesma had the highest callus survival rate (81 %), while the other three cultivars had significantly lower rates (Proteinka-71.3%, Stepa-67.9% and Renesansa-55.3%). Regenerants of all cultivars expressed an increased sensitivity to the presence of sulfonylurea. The sensitivity was especially noticed in treatments II and III, in which only cv. Stepa had 50% of survived plants while the other cultivars had lower survival rates. Treatments II and III had inhibitory effects on callus fresh weight in cultivars Pesma and Renesansa. In cv. Stepa, the callus fresh weight was significantly decreased compared to the control only in the treatment with the highest sulfonylurea concentration. In cv. Proteinka, the callus fresh weight was not significantly decreased by any treatment. Although the studied concentrations of sulfonylurea had no significant effect on dry matter content in the studied development stages, they affected the development and growth of callus tissue.

Key words: herbicide tolerance, wheat, in vitro culture

Corresponding author: Stanislav Šesek, Institute for Field and Vegetable Crops, 21000 Novi Sad, Yugoslavia

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