GENETIKA, Vol. 35, No.3 (2003)
Violeta ANDJELKOVIĆ, Snežana MLADENOVIĆ-DRINIĆ, Milosav BABIĆ, Nenad DELIĆ, and Goran STANKOVIĆ
GENE EXPRESSION PROFILING IN RESPONSE TO HEAT AND WATER STRESS IN MAIZE KERNEL [Abstract] [Full text]
Nenad PAVLOVIĆ, Bogoljub ZEČEVIĆ, Milan ZDRAVKOVIĆ, Miroslav IVANOVIĆ, and Milan DAMJANOVIĆ
VARIABILITY AND HERITABILITY OF AVERAGE YIELD OF ONION BULB (ALLUM CEPA L.) [Abstract] [Full text]
Slavica ČOLIĆ, Gordan ZEC, Dejan MARINKOVIĆ, and Zoran JANKOVIĆ
GENETIC AND PHENOTYPIC VARIABILITY OF CHERRY PLUM (PRUNUS CERASIFERA EHRH.) POMOLOGICAL CHARACTERISTICS [Abstract] [Full text]
Dijana DIJANOVIĆ, Vesna STANKOVIĆ, and Ivan MIHAJLOVIĆ
IMPROVEMENT OF SUNFLOWER FOR CONSUMPTION [Abstract] [Full text]
Jasmina MILENKOVIĆ, R. STANISAVLJEVIĆ, R. SABOVLJEVIĆ, and Ivana STOJANOVIĆ
CORRELATIVE CONNECTION BETWEEN CHARACTERISTICS OF MOTHER LINE AND HYBRID SEED F1 GENERATION OF MAIZE HYBRID ZPTC 196 [Abstract] [Full text]
Radomirka NIKOLIĆ and Nevena MITIĆ
MORPHOLOGICAL CHANGES IN ATYPICAL BIRD’S FOOT TREFOIL PLANTS OBTAINED DURING GENETIC TRANSFORMATION BY AGROBACTERIUM [Abstract] [Full text]
Jovanka ATLAGIĆ, Dejana PANKOVIĆ, and Ana PEKANOVIĆ
BACKCROSSES IN INTERSPECIFIC HYBRIDIZATION IN SUNFLOWER [Abstract] [Full text]
Radovan MARINKOVIĆ, Ana MARJANOVIĆ-JEROMELA, and Dragana VASIĆ
GENETIC VARIABILITY COMPONENTS OF SOME QUANTITATIVE TRAITS OF WINTER OILSEED RAPE (BRASSICA NAPUS L.) [Abstract] [Full text]
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 139-147
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.117
Original scientific paper
GENE EXPRESSION PROFILING IN RESPONSE TO HEAT AND WATER STRESS IN MAIZE KERNEL
Violeta ANDJELKOVIĆ, Snežana MLADENOVIĆ-DRINIĆ, Milosav BABIĆ, Nenad DELIĆ, and Goran STANKOVIĆ
Maize Research Institute “Zemun Polje”, 11185 Belgrade-Zemun, Serbia and Montenegro
Abstract
Andjelković V., S. Mladenović-Drinić, M. Babić, N. Delić, and G. Stanković (2003): Gene expression profiling in response to heat and water stress in maize kernel. – Genetika, Vol. 35, No. 3, 139-147.
New high-throughput techniques, together with advances in analytical and computational technologies are enabling systemic investigations of molecular processes of biological systems. Clustering is widely applied by biologists in genome sequencing projects and phylogenetic studies. In the microarray expression analysis, the aim of cluster is to better organize, but not to alter primary data: genes are visually organized according to the statistical transformation and calculation, due to the expression pattern in maize kernel during heat and water stress. Besides grouping together genes with similar patterns of expression, genes are represented by increased intensity of red, e.g. green color, according to their up- or down-regulation in stress condition, compared to control.
Key words: maize, cluster analysis, microarray, stress
Corresponding author: Violeta Andjelković, Maize Research Institute Zemun Polje, S. Bajića 1, 11185 Belgrade, Serbia and Montenegro,
Phone:**381 11 37 56 704
Fax: **381 11 37 56 707, e-mail: avioleta@mrizp.co.yu
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 149-154
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 635.25
Original scientific paper
VARIABILITY AND HERITABILITY OF AVERAGE YIELD OF ONION BULB (ALLUM CEPA L.)
Nenad PAVLOVIĆ, Bogoljub ZEČEVIĆ, Milan ZDRAVKOVIĆ, Miroslav IVANOVIĆ, and Milan DAMJANOVIĆ
Agricultural Research Institute "Serbia", Belgrade, Center for vegetable crops, S. Palanka, Serbia and Montenegro
Abstract
Pavlović N., B. Zečević, M. Zdravković, M. Ivanović, and M. Damjanović (2003): Variability and heritability of average yield of onion bulb (Allum cepa L.). – Genetika, Vol. 35, No. 3, 149-154.
In order to investigate the variability of bulb yield, the trial was set up on an experimental plot of the Centre for Vegetable Crops in Smederevska Palanka during 1997 and 1998. The trial was performed by applying the method of random block system in five replications. Ten varieties of different geographical origins were included as the research material. The recorded values of the investigated features were determined by applying the variance analysis of a two-factorial trial model 2 (Hadživuković, 1991). The components of the phenotype variance, genotype and phenotype coefficient of variation and heritability in broad sense were estimated according to Singh and Chaudhary (1976). Significant variability was recorded for yield bulbs in both years of investigation. For this parameter, genotype variance was greater than the enviromental. Phenotype coefficient of variation (PCV) was greater than genotype coefficient of variation (GCV). Heritability confirmed that the genotype variability was stronger in the overall phenotype variability.
Key words: onion, yield bulb, index, genotype and phenotype variation coefficient, genotype and environmental variance, heritability
Corresponding author: Nenad Pavlović, Agricultural Research Institute "Serbia", Belgrade, Center for vegetable crops, S. Palanka, Serbia and Montenegro
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 155-160
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
GENETIC AND PHENOTYPIC VARIABILITY OF CHERRY PLUM (PRUNUS CERASIFERA EHRH.) POMOLOGICAL CHARACTERISTICS
Slavica ČOLIĆ, Gordan ZEC, Dejan MARINKOVIĆ, and Zoran JANKOVIĆ
Institut PKB Agroekonomik, 11213 Padinska Skela, Serbia and Montenegro
Abstract
Čolić S., G. Zec, D. Marinković, and Z. Janković (2003): Genetic and phenotypic variability of cherry plum (Prunus cerasifera Ehrh.) pomological characteristics. – Genetika, Vol. 35, No. 3, 155-160.
Cherry plum (Prunus cerasifera Ehrh.) is one of the most widely spread fruit species in our country. The fruits are mostly used for brandy production, and the seed is used for rootstock production in fruit culture. As cherry plum is resistant to plant diseases and pests, chemical protection is not required. Concerning that, cherry plum is reach and cheap source for the production of healthy food. The objective of this research was the analysis of genetic and phenotypic variability, as well as study on correlation of pomological traits of 49 cherry plum genotypes selected from the native population in Serbia. It was measured that the highest genetic variance in total phenotypic variance was for the fruit height and total sugar content. The lowest genetic variance in total phenotypic variance was for the length of the stalk and dry matter content. The highest genetic variance coefficient (CVg = 22.93%) was calculated for the total acid content, although the lowest value of genetic and phenotypic variance was for the fruit width (CVg = 0.69%; CVf = 0.80%). The highest coefficient of phenotypic and genetic correlation was calculated between the weight and height of the fruit. Native population of cherry plum in Serbia and Montenegro is specific because of the extensive variability of the forms, which is highly important for the selection of raw material in breeding process.
Key words: cherry plum, variability, pomological traits
Corresponding author: Slavica Čolić, Institut PKB Agroekonomik, 11213 Padinska Skela, Sebia and Montenegro
tel: 8871-174
e-mail: slavicacol@yahoo.com
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 161-167
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.827
Original scientific paper
IMPROVEMENT OF SUNFLOWER FOR CONSUMPTION
Dijana DIJANOVIĆ, Vesna STANKOVIĆ, and Ivan MIHAJLOVIĆ
Agricultural Research Institute “Srbija”, Belgrade
Agricultural and Technological Resesarch Center Zaječar, Zaječar, Serbia and Montenegro
Abstract
Dijanović D., V. Stanković, and I. Mihajlović (2003): Improvement of sunflower for consumption. – Genetika, Vol. 35, No. 3, 161-167.
Growing sunflower (Heliianthus annuus L.) for consumption is becoming more and more attractive in the whole world and in our country, as well. The consumption of this product has been considerably increased because it is being used more and more in various forms: sunflower seed in a hull, hulled kernels fried or non-fried, salted or non-salted with addition of various spices. The sunflower kernel is used for preparing over 100 different food products: special types of bread, cakes, ice-cream, chocolate. In accordance with the trade demands, the aims of sunflower seeds for consumption improvement are: high productivity, greater content of proteins, lesser content of oil, oncreased mass of 1000 seeds, lesser portion of the hull, easy nibbling, as well as tolerance to dominant diseases in the growing region. In the Agricultural and Technological Research Center in Zajecar, 4 genotypes of protein sunflower (two cultivars and two hybrids) have been created so far. This study shows the results achieved in increasing the content of protein, the mass of 1000 seeds, decreasing the content of oil and hull of the new 90 hybrid combination. The hybrid combinations were obtained by crossing of the CMS and restorer lines.
Key words: sunflower for consumatiom, hybrid combinations, protein content, oil content, mass of 1000 seeds, hull content
Corresponding author: Dijana Dijanović, Agricultural Research Institute “Srbija”, Belgrade Agricultural and Technological Resesarch Center Zaječar, Zaječar, Grljanski put bb, 19000 Zaječar, Serbia and Montenegro
Tel:+381 19 420 222
Fax:+381 19 421 668
E-mail czpiti@ptt.yu
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 169-176
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
CORRELATIVE CONNECTION BETWEEN CHARACTERISTICS OF MOTHER LINE AND HYBRID SEED F1 GENERATION OF MAIZE HYBRID ZPTC 196
Jasmina MILENKOVIĆ1, R. STANISAVLJEVIĆ1, R. SABOVLJEVIĆ2, and Ivana STOJANOVIĆ1
1 Agricultural Research Institute ”SERBIA”, Agricultural and Technological Research Center, Grljanski put bb, 19000 Zaječar
2 Faculty of Agriculture, Nemanjina 6, 11000 Belgrade, Serbia and Montenegro
Abstract
Milenković J., R. Stanisavljević, R. Sabovljević, and I. Stojanović (2003): Correlative connection between characteristics of mother line and hybrid seed F1 generation of maize hybrid ZPTC 196. – Genetika, Vol. 35, No. 3, 169-176.
Production of the maize hybrid seed (Zea mays L.) in the area of the town of Zaječar and its surroundings is limited by the ecological conditions. The loss of precipitations and high air temperatures during summer do not allow the hybrid seed of later vegetation to be produced. However, reduced amount of precipitations in the period of time from August to September, with favourable heat conditions, provide mature and healthy seed material only if the mother line has shorter vegetation period (FAO 200 - 300). This study examines the influence of the cob corn characteristics (cob length, number of seeds on a cob, mass of the seeds on a cob, number of the seeds in a fraction and germination of natural seed material of the maize hybrid ZPTC 196 seed. The applied correlative analysis involved all experimental conditions (two years, plant rows of the mother line, with watering and without watering). The results of the investigation showed that the applied approach of correlative analysis for the fractional content of a cob corn (number and mass per fractions) almost owerwhelming, as far as the seed germination is concerned, it was probable that all factors having influence on that characteristic were not included.
Key words: correlative connection, hybrid seed, maize, mother line
Corresponding author: Jasmina Milenković, ARI “SERBIA”, Agricultural and Technological Research Center, Grljanski put bb, 19000 Zaječar, Serbia and Montenegro. Tel: +381 19 420 222; Fax: +381 19 421 668; Email: czpiti@ptt.yu
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 177-185
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 633.2
Original scientific paper
MORPHOLOGICAL CHANGES IN ATYPICAL BIRD’S FOOT TREFOIL PLANTS OBTAINED DURING GENETIC TRANSFORMATION BY AGROBACTERIUM
Radomirka NIKOLIĆ and Nevena MITIĆ
ARI “Serbia”, Agricultural and Technological Research Centre, 19000 Zaječar,
Serbia and Montenegro
Abstract
Nikolić R. and N. Mitić (2003): Morphological changes in atypical bird’s foot trefoil plants obtained during genetic transformation by Agrobacterium. – Genetika, Vol. 35, No. 3, 177-185.
Atypical plants of bird’s foot trefoil (Lotus corniculatus L., Bokor cv.) showing altered morphological characters that deviate from a normal phenotype were found after plant regeneration from transformed tissue. It had been obtained by genetic transformation of root sections of seedlings using Agrobacterium tumefaciens vector LBA4404/pBI121 on a medium supplemented with 0.2 mg l-1 BAP. The transformants 2b and 4a were found to have a greatly atypical habit, including shortened internodes, elongated leaves, regular leaf arrangement along the stem and thicker leaves. Inheritance of altered characters was observed in the first progeny generation, and their genetic origin was considered.
Key words: Lotus corniculatus, leguminous plants, in vitro regeneration, genetic transformation, somaclonal variation, phenotypic variants
Corresponding author: Radomirka Nikolić, ARI “Serbia”, Agricultural and Technological Research Centre, 19000 Zaječar, Serbia and Montenegro
Phone: 019/420-222, Fax: 019/421-668
e-mail: czpiti@ptt.yu
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 187-197
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.222.7
Original scientific paper
BACKCROSSES IN INTERSPECIFIC HYBRIDIZATION IN SUNFLOWER
Jovanka ATLAGIĆ1, Dejana PANKOVIĆ1, and Ana PEKANOVIĆ2
1 Institute of Field and Vegetable Crops, 21000 Novi Sad,
2 Faculty of Biology, 11000 Belgrade, Serbia and Montenegro
Abstract
Atlagić J., D. Panković, and A. Pekanović (2003): Backcrosses in interspecific hybridization in sunflower. – Genetika, Vol. 35, No. 3, 187-197.
When incorporating desirable traits (resistance to causal agents of various diseases) from the wild relatives into the cultivated sunflower, some undesirable ones are introduced too (branching, small head diameter, low oil content, etc.). To overcome this problem, backcrosses (F1 interspecific hybrids x cultivated sunflower) are used, although very often desirable traits are lost in the process. Cytological analysis (meiosis and pollen viability) and molecular markers (RAPD) were used to estimate what portion of the parental species genome was present in the interspecific hybrids of the F1 and BC1F1 generations. The results showed that the percentage of irregularities at meiosis increased from F1 to BC1F1 gen. They also indicated the presence of aneuploids and sterility in the cross between the hexaploid species H.rigidus and cultivated sunflower. The genetic distance between the parents was 83%, that between H.rigidus and the F1 hybrid 54 - 61%, and that between H.annuus and F1 hybrid 70-76%. In the BC1F1 generation, the genetic distance from H.annuus decreased to 58-66% and that from H.rigidus increased to 69-76%.
Key words: Sunflower, interspecies, backcrosses, morphology, cytogenetics and molecular markers
Corresponding author: Jovanka Atlagić, Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Tel: 381 021 4898 401
e-mail: atlagic@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 35, No.3 (2003), pp. 199-205
© 2003 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22
Original scientific paper
GENETIC VARIABILITY COMPONENTS OF SOME QUANTITATIVE TRAITS OF WINTER OILSEED RAPE (BRASSICA NAPUS L.)
Radovan MARINKOVIĆ, Ana MARJANOVIĆ-JEROMELA, and Dragana VASIĆ
Institute of Field and Vegetable Crops, 21000 Novi Sad, Maksima Gorkog 30, Serbia and Montenegro
Abstract
Marinković R., A. Marjanović-Jeromela, and D. Vasić (2003): Genetic variability components of some quantitative traits of winter oilseed rape (Brassica napus L.). – Genetika, Vol. 35, No. 3, 199-205.
Analysis of genetic variance components for number of leaves and branches per plant and stem diameter was done according to the method of Hayman (1954). Heritability in narrow (h2a) and broad (h2b) sense was determined for the same traits, using the method of Mather and Jinks (1971). Non-additive component of genetic variance was greater than additive component in all three studied traits. Dominant and recessive genes were not equally distributed in parent genotypes, with dominant genes prevailing. Ratio (H1/D)1/2 was higher than 1 in all three tested traits. Calculated values for heritability in narrow sense showed that stem diameter and number of branches per plant are traits with low heritability, and number of leaves per plant a trait with the high heritability. Heritability in a broad sense was high for all three tested traits.
Key words: cultivar, diallel crosses, heritability, additivity, dominance
Corresponding author: Radovan Marinković, Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Phone: 381 21 4898100; fax: 381 21 641212; e-mail: radomar@ifvcns.ns.ac.yu