GENETIKA, Vol. 36, No.3 (2004)
Nevena MITIĆ and Radomirka NIKOLIĆ
INHERITANCE OF IN VITRO RESPONSE IN WHEAT [Abstract] [Full text]
Nevena NAGL, Snežana MEZEI, Lazar KOVAČEV, Dragana VASIĆ, and Nikola ČAČIĆ
INDUCTION AND MICROPROPAGATION POTENTIAL OF SUGAR BEET HAPLOIDS [Abstract] [Full text]
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Ljiljana STANKOVIĆ, Milan ZDRAVKOVIĆ, and Milan DAMJANOVIĆ
SENESCENCE OF RIN, rin/rin, rin/+ AND +/+ TOMATO FRUITS [Abstract] [Full text]
Victor M. SALCEDA
THE FREQUENCY OF ALLELIC LETHALS AND COMPLEMENTATION MAPS IN NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER FROM MEXICO [Abstract] [Full text]
Dijana DIJANOVIĆ, Marija KRALJEVIĆ- BALALIĆ, Vesna STANKOVIĆ, and Ivan MIHAJLOVIĆ
PHENOTYPIC STABILITY OF YIELD COMPONENTS IN PROTEIN SUNFLOWER [Abstract] [Full text]
Jelena ALEKSIĆ and Saša ORLOVIĆ
EX SITU CONSERVATION OF GENETIC RESOURCES OF FIELD ELM (ULMUS MINOR MILL.) AND EUROPEAN WHITE ELM (ULMUS LAEVIS PALL.) [Abstract] [Full text]
Milka BRDAR, Marija KRALJEVIĆ BALALIĆ, and Borislav KOBILJSKI
OBSERVED DURATION AND AVERAGE AND MAXIMUM GRAIN FILLING RATES IN WHEAT GENOTYPES OF DIFFERENT EARLINESS [Abstract] [Full text]
Slaviša STOJKOVIĆ, Nebojša DELETIĆ, Milan BIBERDŽIĆ, and Jovanka STOJANOVIĆ
VARIABILITY OF DRY SUBSTANCE ACCUMULATION AND UTILIZATION PARAMETERS OF WINTER WHEAT PLANT [Abstract] [Full text]
Irina BOŽOVIĆ, Milica RADOSAVLJEVIĆ, Slađana ŽILIĆ, and Rade JOVANOVIĆ
A GENETIC BASE OF UTILISATION OF MAIZE COB AS A VALUABLE NATURALLY RENEWABLE RAW MATERIAL [Abstract] [Full text]
Mirjana ŠIJAČIĆ-NIKOLIĆ and Vasilije ISAJEV
ASSESSMENT OF HETEROTIC EFFECT IN SERBIAN SPRUCE HYBRID COMBINATIONS [Abstract] [Full text]
Zorica PAJIĆ, Lana ĐUKANOVIĆ, and Uroš ERIĆ
EFFECT OF ENDOSPERM MUTANTS ON MAIZE SEED GERMINATION [Abstract] [Full text]
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 181-186
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.1: 633.11
Original scientific paper
INHERITANCE OF IN VITRO RESPONSE IN WHEAT
Nevena MITIĆ and Radomirka NIKOLIĆ
Agricultural Research Institute “Serbia”, Center for Agricultural and Technological Research, 19000 Zaječar, Serbia and Montenegro
Abstract
Mitić N. and R. Nikolić (2004): Inheritance of in vitro response in wheat. – Genetika, Vol. 36, No. 3, 181-186.
The inheritance of in vitro culture response was studied by using immature embryos from five wheat cultivars and their reciprocal hybrids. In vitro culture response was evaluated according to callus formation, percentage of regenerative calli and the number of plants per embryo. By crossing the cultivar Vesna (VS) with highest tissue culture response and the two cultivars with lowest response Leda (LD) and Zajecarska 65 (ZA), it was demonstrated that the regeneration potential was heritable. VS as female parent, enhanced regeneration response in hybrids VSxLD and VSxZA, while as a male parent, VS did not affect the regeneration ability of hybrids LD and ZA. However, hybrids having LD and ZA as a male parents exhibited a decreased regeneration potential, as compared to self-pollinated VS. The results suggest the presence of a class of extra-nuclear factors in the VS cultivar. They significantly account for relatively higher regeneration capacity in the hybrids having this cultivar as a female parent than in those where the VS was male parent.
Key words: Triticum aestivum, callus formation, in vitro regeneration potential, maternal effects, average dominance effects
Corresponding author: Nevena Mitić, ARI “Serbia”, Center for Agricultural and Technological Research, Grljanski put b.b., 19000 Zaječar
Phone: 019/420-222, Fax: 019/421-668; e-mail: czpiti@ptt.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 187-194
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.11: 633.63
Original scientific paper
INDUCTION AND MICROPROPAGATION POTENTIAL OF SUGAR BEET HAPLOIDS
Nevena NAGL, Snežana MEZEI, Lazar KOVAČEV, Dragana VASIĆ, and Nikola ČAČIĆ
Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Abstract
Nagl N., S. Mezei, L. Kovačev, D. Vasić, and N. Čačić (2004): Induction and micropropagation potential of sugar beet haploids. – Genetika, Vol. 36, No. 3, 187-194.
The aim of research was obtaining sugar beet haploids via gynogenesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cytokinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each subcultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes.
Key words: Beta vulgaris L., ovule, haploid, micropropagation
Corresponding author: Nevena Nagl, Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Phone:++381 21 4898 327, Fax: ++381 21 4898 333, e-mail: nagl@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 195-203
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 588.145.2: 635.64
Original scientific paper
SENESCENCE OF RIN, rin/rin, rin/+ AND +/+ TOMATO FRUITS
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Ljiljana STANKOVIĆ, Milan ZDRAVKOVIĆ, and Milan DAMJANOVIĆ
Agricultural Research Institute “Serbia”, Centre for Vegetable Crops,
11420 Smederevska Palanka, Serbia and Montenegro
Abstract
Zdravković J., Ž. Marković, Lj. Stanković , M. Zdravković, and M. Damjanović (2004): Maturing and senescence of rin, rin/rin, rin/+ and +/+ tomato fruits. – Genetika, Vol. 36, No. 3, 195-203.
Ripening inhibitor (rin) gene is a spontaneous recessive mutant which changes fruit ripening aspects (most important synthesis of carotene, especially lycopene). It also delays fruit senescence. Tomato is a vegetable crop with specific maturing climax (pik). Tomato genotypes homozygote for rin gene does not have this maturing climax, so the ethylene production and red, lycopene colour does not appear. In order to research the maturing process material from the final tomato selection cycle we used: pure line S-49 (genetic constitution u/u, with uniform ripening), line hom 4 (rin/rin, homozygote with ripening inhibitor) and hybrid combination 449 F1 (u/rin, heterozygote for ripening inhibitor). Fruits with uniform ripening and hybrids ripened simultaneously, while homozygote with rin gene did not ripe at all. Ethylene stimulates the appearance of yellow colour in rin fruits and the lycopene production. After treatment with ETEPHONE (0.1%) (Ethylene) fruits with uniform ripening, senescence more quickly, while hybrids senescence slowly. Rin homozygotes did not change colour, but the fruits senescence more quickly comparing to control. Ethylene treatment speeded the maturing and senescence process in tomato fruits. Extended maturing process as a result influence of rin gene, makes the new created hybrids a late maturing ones. On the other hand, the firmness of fruits is improved as well as the »shelf life«, which enables longer transportation and storing, coordinated maturing according to market demands. The aim was to research the maturing process of rin heterozygote, and the reaction of some genotypes to treatment with ETREL (Ethylene) in order to decrease extremely late maturing.
Key words: tomato, fruit maturing and senescence, uniform maturing, effects of ETEPHONE
Corresponding author: Jasmina Zdravković, Agricultural Research Institute “Serbia”, Centre for Vegetable Crops , 71 Karađorđeva St, 11420 Smederevska Palanka, Serbia and Montenegro.
Phone: + 381 26 323 170; Fax: + 381 26 323 785
e-mail: jzdravkovic@cvcsp.co.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 205-212
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.2: 595.773.4
Original scientific paper
THE FREQUENCY OF ALLELIC LETHALS AND COMPLEMENTATION MAPS IN NATURAL POPULATIONS OF DROSOPHILA MELANOGASTER FROM MEXICO
Victor M. SALCEDA
Departamento de biologia, Instituto nacional de investigaciones nucleares. Apartado Postal 18-1027, México, 11801, D.F. Mexico
Abstract
Salceda V.M. (2004): The frequency of allelic lethals and complementation maps in natural populations of Drosophila melanogaster from Mexico. – Genetika, Vol. 36, No. 3, 205-212.
Departing from a previous study on the genetic loads affecting the second chromosome of Drosophila melanogaster in four natural populations, 171 lethal chromosomes were recovered and maintained as a balanced stocks in the condition Cy L / l (l=lethal); of those lethals 24 correspond to population A, 50 to populations B and C and 47 to population D. later on an intra-population allelism test for the four populations was performed for each one. A total of 3807 inter lethal crosses were done yielding a total of 110 allelic combinations, from them the respective percentage of allelism for each population was calculated and they are as follow: 3.98 % for population A, 1.80 % for population B, 3.67 % for population C and 2.96 % for population D. the observed values for the frequency of allelism in these populations are not significantly different from those reported by other authors in similar studies in natural and/or experimental populations. Beside these values the frequency for singles, doubles, triplets and even quadruplets present in each population were determined, they shown the presence of various complementation maps due to the clustering of few different lethals; also a large complementation map formed by a large cluster involving the presence of 26 different lethals found in population D all of them combined constituting a single unit was found.
Key words: genetic load, allelism, complementation map, Drosophila
Corresponding author: Victor M. Salceda, Departamento de biologia, Instituto nacional de investigaciones nucleares. Apartado Postal 18-1027, México, 11801, D.F. Mexico.
e-mail: vmss@nuclear.inin.mx
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 213-220
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
PHENOTYPIC STABILITY OF YIELD COMPONENTS IN PROTEIN SUNFLOWER
Dijana DIJANOVIĆ1, Marija KRALJEVIĆ- BALALIĆ2, Vesna STANKOVIĆ1, and Ivan MIHAJLOVIĆ1
1 Agricultural Research Institute “Srbija”, Belgrade, Agricultural & Technological Resesarch Center Zaječar,
2 University of Novi Sad, Faculty of Agriculture Novi Sad, Serbia and Montenegro
Abstract
Dijanović D., M. Kraljević- Balalić, V. Stanković, And I. Mihajlović (2004): Phenotypic stability of yield components in protein sunflower. – Genetika, Vol. 36, No. 3, 213-220.
Phenotypic stability of the yield components of three sunflower (Helianthus annuus L.) inbred lines in their third, fourth and fifth year of self-pollination was analysed. Stability parameters were calculated following an Eberhart and Russell (1966) model. According to regression coefficients, the investigated lines showed satisfactory stability of the protein content in seeds and weight per 1000 seeds. The line D4441 in the third year of self-pollination was identified as the most stabile one regarding protein content. As for weight per 1000 seeds, the best genotype stability was detected in the line Rs 4 I 10 in its fourth year of self-pollination.
Key words: sunflower, phenotypic stability, regression coefficient, protein content, weight per 1000 seeds
Corresponding author: Dijana Dijanovic, Agricultural Research Institute “Srbija”, Belgrade Agricultural & Technological Resesarch Center Zajecar, Grljanski put bb, 19000 Zajecar, Serbia and Montenegro
Phone: +381 19 420 222; Fax:+381 19 421 668; e-mail czpiti@ptt.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 221-227
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
EX SITU CONSERVATION OF GENETIC RESOURCES OF FIELD ELM (ULMUS MINOR MILL.) AND EUROPEAN WHITE ELM (ULMUS LAEVIS PALL.)
Jelena ALEKSIĆ and Saša ORLOVIĆ
University of Novi Sad, Faculty of Agriculture, Institute of Lowland Forestry and Environment, Antona Čehova 13, Novi Sad, Serbia and Montenegro
Abstract
Aleksić J. and S. Orlović (2004): Ex situ conservation of genetic resources of field elm (Ulmus minor Mill.) and european white elm (Ulmus laevis Pall.). – Genetika, Vol. 36, No. 3, 221-227.
Principles of the conservation of genetic resources of elms (Ulmus spp.) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare, endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm (Ulmus minor Mill.) and European white elm (Ulmus laevis Pall.) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.
Key words: Ulmus, ex situ conservation, in vitro
Corresponding author: Jelena Aleksić, University of Novi Sad, Faculty of Agriculture, Institute of Lowland Forestry and Environment, Antona Čehova 13, Novi Sad, Serbia and Montenegro.
Tel: +381 21 540 383; Fax: +381 21 540 383; E-mail: jelenaa@polj.ns.ac.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 229-235
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 633.11
Original scientific paper
OBSERVED DURATION AND AVERAGE AND MAXIMUM GRAIN FILLING RATES IN WHEAT GENOTYPES OF DIFFERENT EARLINESS
Milka BRDAR1, Marija KRALJEVIĆ BALALIĆ2, and Borislav KOBILJSKI3
1 Schoolarship holder of the Ministry of Science and Environmental Protection, Stevana Divnina Babe 8, 21000 Novi Sad,
2 Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Trg D. Obradovića 8
3 Institute of Field and Vegetable Crops, 21000 Novi Sad, Maksima Gorkog 30
Abstract
Brdar M., M. Kraljević Balalić, and B. Kobiljski (2004): Observed duration and average and maximum grain filling rates in wheat genotypes of different earliness. – Genetika, Vol. 36, No. 3, 229-235.
Grain yields of wheat (Triticum aestivum L.) are influenced partly by final grain dry weight, which is largely determined by the rate and duration of the grain filling process. A study was undertaken to compare the observed final grain dry weight of five groups of wheat genotypes differing in earliness (extra early, medium early, medium late, late and a control group of high yielding NS cultivars) with the observed duration and average and maximum rates of grain filling in two different environments. Correlation coefficients were used to determine which grain filling parameter had more influence on final grain dry weight. In an environment common for our country (2002), final grain dry weight was strongly positively correlated with the average and maximum rates and strongly negatively correlated with the duration of grain filling. The medium late and control groups had the highest final grain dry weights. Correlations between final grain dry weight and the duration and average rate of grain filling were positive in an unfavorable environment (2001). The NS cultivars and extra early genotypes had the highest final grain dry weights. The rate and duration of grain filling are usually negatively correlated. The influence of grain filling parameters on final grain dry weight is not the same in different environments, so the ability of the genotype to compensate for the low grain filling rate with grain fill prolongation in unfavorable environments might be more important. The observed average grain filling rate is probably more important as a parameter for describing these processes than the maximum one.
Key words: wheat, grain filling duration, grain filling rate
Corresponding author: Milka Brdar, Stevana Divnina Babe 8, 21000 Novi Sad;
e-mail: milkabrdar@yahoo.com
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 237-243
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 633.11
Original scientific paper
VARIABILITY OF DRY SUBSTANCE ACCUMULATION AND UTILIZATION PARAMETERS OF WINTER WHEAT PLANT
Slaviša STOJKOVIĆ1, Nebojša DELETIĆ1, Milan BIBERDŽIĆ1, and Jovanka STOJANOVIĆ2
1University of Priština, Faculty of Agriculture, Lešak;
2Institute of Agricultural Research “Serbia”, Centre for Small Grains, Kragujevac, Serbia and Montenegro
Abstract
Stojković S., N. Deletić, M. Biberdžić, and J. Stojanović (2004): Variability of dry substance accumulation and utilization parameters of winter wheat plant. – Genetika, Vol. 36, No. 3, 237-243.
Some dry substance accumulation and utilization parameters of wheat plant and their influence on grain yield have been studied through a field trial with 20 winter wheat cultivars. The studied parameters value (biological yield, dry substance reutilization, number of grains per spike, 1000 grain mass, plant height, etc.) varried depending of genotypic specificity and environmental conditions. Grain yield was influenced not only by the total dry substance accumulation in plant (i.e. biological yield), but also by this accumulation amount before and after flowering. Yield forming was affected by many parameters, and cultivar range was different for various parameters. The best grain yield was observed in cultivars having elevated values of more than few parameters. The highest mean grain yield was observed in cultivar Tiha, and also high grain yield values were found in cultivars Gruža, Nevesinjka, and Toplica.
Key words: winter wheat, dry substance, utilization parameters
Corresponding author: S. Stojković, University of Pristina, Faculty of Agriculture, Lešak, Serbia and Montenegro
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 245-256
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 633.15
Original scientific paper
A GENETIC BASE OF UTILISATION OF MAIZE COB AS A VALUABLE NATURALLY RENEWABLE RAW MATERIAL
Irina BOŽOVIĆ, Milica RADOSAVLJEVIĆ, Slađana ŽILIĆ, and Rade JOVANOVIĆ
Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Belgrade-Zemun, Serbia and Montenegro
Abstract
Božović I., M. Radosavljević, S. Žilić, R. Jovanović (2004): A genetic base of utilisation of maize cob as a valuable naturally renewable raw material. – Genetika, Vol. 36, No. 3, 245-256.
The original technological method of the maize cob processing has been developed at the Maize Research Institute, Zemun Polje, by which lignocellulose granules of different particle sizes are produced from the cob. Different chemical composition and physical and chemical properties of these fractions, and especially a great capacity of binding liquids, particularly oil and water determine, their usage as degreasing and drying means. Due to their great hardness and abrasive capacity, products made from ground cobs are usable for polishing in the metal processing industry, while the composition of certain compounds (pento-san) are of a particular importance in the chemical industry for the pro duction of furfural and its derivates. As these products are inert, of neutral pH and free of heavy metals they are used as organic carriers in the pro duction of pesticides and agro-chemicals, as well as, in cosmetics and the pharmaceutical industry.
Key words: maize cob, lignocellulose granule, celgran products, ab sorption, degradability, utility value
Corresponding author: Milica Radosavljević, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade-Zemun, Serbia and Montenegro
e-mail: rmilica@mrizp.co.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 257-263
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.222; 630.232; 582.47
Original scientific paper
ASSESSMENT OF HETEROTIC EFFECT IN SERBIAN SPRUCE HYBRID COMBINATIONS
Mirjana ŠIJAČIĆ-NIKOLIĆ and Vasilije ISAJEV
Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade, Serbia and Montenegro
Abstract
Šijačić-Nikolić M. and V. Isajev (2004): Assessment of heterotic effect in serbian spruce hybrid combinations. – Genetika, Vol. 36, No. 3, 257-263.
The occurrence and magnitude of heterotic effect was determined among hybrid combinations and parent individuals obtained by open pollination in Serbian spruce seedling seed orchard at Godovik. The heterotic effect was analysed for cone length and width and the average number of seeds per cone. The study results show the different heterotic effect, both for the same trait in different hybrid combinations and for different traits of the same hybrid.
Key words: Serbian spruce, hybrid combinations, heterosis
Corresponding author: Mirjana Šijačić-Nikolić, Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade, Serbia and Montenegro
Tel. 011-3553-122
e-mail: sijacic@ptt.yu
Published in „GENETIKA“ Vol. 36, No.3 (2004), pp. 265-270
© 2004 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.822: 635.67
Original scientific paper
EFFECT OF ENDOSPERM MUTANTS ON MAIZE SEED GERMINATION
Zorica PAJIĆ, Lana ĐUKANOVIĆ, and Uroš ERIĆ
Maize Research Institute “Zemun Polje”, 11185 Zemun, Serbia and Montenegro
Abstract
Pajić Z., L. Đukanović, and U. Erić (2004): Effect of endosperm mutants on maize seed germination. – Genetika, Vol. 36, No. 3, 265-270.
The expression of genetic potential of yielding and quality of a certain genotype depends among other factors on seed quality. Seed is very important not only for the reproduction of the particular plant species, but also, for the contemporary plant production. Each part of maize seed (pericarp, endosperm and germ) has a specific function in the complex process of germination and emergence. The following three genotypes of different endosperm types were observed: ZPSC 42A (standard grain quality dent hybrid), ZPSC 504 su (sweet maize hybrid with a sugary gene) and ZPSyn.II sh2 (synthetic population with a shrunken2 gene). Seed viability of the stated genotypes was determined by the accepted ISTA methods: standard method, accelerating age and cold test. Obtained results point out to differences in the germination capacity of the observed genotypes. The greatest reduction of the germination capacity and the emergence rate was expressed by the application of the accelerating ageing method. Appeared differences are probably a result of the endosperm texture (type), grain weight, sugar content and pericarp thickens and composition.
Key words: Maize seed, sweet maize, seed germination
Corresponding author: Zorica Pajić, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun, Serbia and Montenegro
e-mail: zpajic@mrizp.co.yu