GENETIKA, Vol. 37, No.1 (2005)

 

Vesna VUJAČIĆ

VARIABILITY AND FACTOR ANALYSIS OF MORPHOLOGICAL AND PRODUCTIVE CHARACTERISTICS OF SPECIES OF THE GENUS AMARANTHUS [Abstract] [Full text]

 

Slavica KOŠARČIĆ, A. KOVACS, D. KOŠARČIĆ, Ljiljana SUVAJDŽIĆ, Mira KOVAČEVIĆ, M. KAPETANOV, Dubravka MILANOV, and Milica ŽIVKOV BALOŠ

CHROMOSOME CHANGES IN CATTLE ON THE FARMS IN SERBIA [Abstract] [Full text]

 

Biljana GORJANOVIĆ and Marija KRALJEVIĆ-BALALIĆ

INHERITANCE OF PLANT HEIGHT AND SPIKE LENGHT IN WHEAT [Abstract] [Full text]

 

Radomirka NIKOLIĆ and Nevena MITIĆ

ANALYSIS OF MORPHOLOGICAL TRAITS OF BIRD’S FOOT TREFOIL PLANTS CV. BOKOR TRANSFORMED WITH AGROBACTERIUM RHIZOGENES [Abstract] [Full text]

 

Rade MILETIĆ, Mihajlo ŽIKIĆ, Nevena MITIĆ, and Radomirka NIKOLIĆ

POMOLOGICAL AND TECHNOLOGICAL CHARACTERISTICS OF COLLECTED SELECTIONS OF CHERRY PLUM PRUNUS CERASIFERA ERHR. [Abstract] [Full text]

 

Sreten TERZIĆ and Jovanka ATLAGIĆ

PHENOTYPE VARIABILITY AND INHERITANCE OF LEAF SHAPE IN F1 GENERATION OF SUNFLOWER [Abstract] [Full text]

 

Sandra GVOZDENOVIĆ, Jovan JOKSIMOVIĆ, Dragan ŠKORIĆ

GENE EFFECT AND COMBINING ABILITIES FOR PLANT HEIGHT AND HEAD DIAMETER IN SUNFLOWER [Abstract] [Full text]

 

Milan ZDRAVKOVIĆ, Jasmina ZDRAVKOVIĆ, Ljiljana STANKOVIĆ, and Nenad PAVLOVIĆ

COMBINING ABILITIES OF INHERITING FIRST POD HEIGHT OF SOME FRENCH BEAN LINES (PHASEOLUS VULGARIS L.) [Abstract] [Full text]

 

Nebojša DELETIĆ, Slaviša STOJKOVIĆ, Vladan ĐURIĆ, Slaviša GUDŽIĆ, and Milan BIBERDŽIĆ

THE EFFECT OF A HIGH SELECTION INTENSITY ON THE CHANGE OF MAIZE YIELD COMPONENTS’ ADDITIVE VARIANCE [Abstract] [Full text]

 

Ivana STRAHINIĆ, Jelena BEGOVIĆ, Đorđe FIRA, Mihailo OSTOJIĆ, and Ljubiša TOPISIROVIĆ

ANALYSIS OF NATURAL ISOLATES OF LACTOBACILLI RESISTANT TO BACTERIOCIN NISIN [Abstract] [Full text]

 

Predrag JOVIN, Milorad ROŠULJ, Miodrag TOLIMIR, and Jasmina MILENKOVIĆ

EFFECTS OF THE SOWING DENSITY ON THE YIELD AND THE NUMBER OF GERMINATED SEEDS IN SEED MAIZE [Abstract] [Full text]



 

Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 1-13                                           

© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21

doi:10.2298/GENSR0501001V

Original scientific paper

VARIABILITY AND FACTOR ANALYSIS OF MORPHOLOGICAL AND PRODUCTIVE CHARACTERISTICS OF SPECIES OF THE GENUS AMARANTHUS

Vesna VUJAČIĆ

 

Faculty of Agriculture, 11080 Zemun, Serbia and Montenegro

 

 

Abstract

 

Vujačić V. (2005): Variability and factor analysis of morpho­logical and productive characteristics of species of the genus Amaran­thus. – Genetika, Vol. 37, No. 1, 1-13.

Ten genotypes of amaranth were being studied for three years. Morphological and productive characteristics - plant height, foliage per plant, average foliage length, average foliage width, mass per plant, and seed mass per plant were the subject of this research. Variability of these traits was analyzed and classification of the genotypes by the method of major components was conducted. Variability within a specific trait was significant. In case of the plant height it ranged between 93.18 cm (geno­type 9 - A. cruentus) and 160.78 cm (genotype 1 - A mantegazzianus); fo­liage per plant raged between 12.89 (genotype 10 - A cruentus) and 23.46 (genotype 1 - A mantegazzianus); average foliage length varied from 14.77 cm (genotype 9 - A cruentus) to 26.72 cm (genotype 1 - A mantegazzianus); average foliage width ranged between 6.30 cm (geno­type 9 - A cruentus) and 14.46 cm (genotype 1 - A mantegazzianus); foli­age mass per plant ranged between 94.05 g (genotype 3 - A molleros) and 246.81 g (genotype 1 - A mantegazzianus). Seed mass per plant varied from 45.56 g (genotype 3 - A molleros) to 67.55 g (genotype 1 - A mantegazzianus). The major components method, i.e. factor analysis indi­cated that the characteristics such as: plant height, average foliage length and average foliage width, had a significant factor loading with the first factor. These traits are of a crucial importance for genotype variability. Foliage number and foliage mass were significantly correlated with the second factor, meaning that they were of a minor importance for the genotype variability. Such results offer guidance with respect to the plant modeling, i.e. indicate how to proceed with the breeding program of this species.

Key words: genotype, amaranth, foliage, seeds, variability, major com­ponents method

 

Corresponding author: Vesna Vujačić, Faculty of Agriculture, 11080 Zemun, Serbia and Montenegro

e-mailwesnaw2002@hotmail.com

 

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 15-23
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.11: 636.2

doi:10.2298/GENSR0501015K

Original scientific paper

 

 

CHROMOSOME CHANGES IN CATTLE ON THE FARMS IN SERBIA

 

Slavica KOŠARČIĆ1, A. KOVACS3, D. KOŠARČIĆ2, Ljiljana SUVAJDŽIĆ1, Mira KOVAČEVIĆ1, M. KAPETANOV1, Dubravka MILANOV1, and Milica ŽIVKOV BALOŠ1

 

1 Scientific Veterinary Institute "Novi Sad", Rumenački put 20, Novi Sad,

2 Institute for Reproduction and Artificial Insemination of Domestic Animals, Temerin, Serbia and Montenegro

3 Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary

 

 

Abstract

 

Košarčić S., A. Kovacs, D. Košarčić, Lj. Suvajdžić, M. Kovačević, M. Kapetanov, D. Milanov, and M. Živkov Baloš (2005): Chromosome changes in cattle on the farms in serbia. – Genetika, Vol. 37, No. 1, 15-23.

In the paper we presented five-year investigations of numeric and structural changes in cattle karyotype on five farms and two centers for reproduction in Serbia. There were 371 breeding animals (215 male and 156 female), out of which 267 Holstein Friesian breed (193 male and 74 female), 62 Simmental (17 male and 45 female) and 42 Grey Steppe breed (5 male and 37 female). Cultivating of lymphocytes and karyotype analyses, according to the international standards for karyotypization of domestic animals, were applied. The aim of the investigation was to test genetic material on chromosome level of animals introduced into reproduction. The following changes were discovered in the karyotype: 6 animals of Holstein-Friesian breed were with chimeras 2n=60XX/XY and there was one Robertson’s translocation in Simmental breed. Structural changes as breakage and a ring were discovered in two animals of Holstein-Friesian and Simmental breed raised in the area of bombing in Serbia. The animals of Grey Steppe breed had normal karyotype. There is a total of 9 animals with changes (2.42%), while 362 were with normal karyotype, total 97.57%. According to the results, it may be concluded that cytogenetical attestation of the breeding animals will have to be continued for the purpose of protecting the genofond on cattle farms.

Key words: cattle, cytogenetic attestation, himeras, karyotype, translocation

 

 

Corresponding author: Slavica Košarčić, Scientific Veterinary Institute "Novi Sad", Rumenački put 20, Novi Sad, Serbia and Montenegro

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 25-31
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21: 633.11

doi:10.2298/GENSR0501025G

Original scientific paper

INHERITANCE OF PLANT HEIGHT AND SPIKE LENGHT IN WHEAT

Biljana GORJANOVIĆ1 and Marija KRALJEVIĆ-BALALIĆ2

 

1 Primary school “Dušan Jerković”, 22320 Inđija,

2 Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Serbia and Montenegro

 

 

Abstract

 

Gorjanović B. and M. Kraljević-Balalić (2005): Inheritance of plant height and spike lenght in wheat. – Genetika, Vol. 37, No. 1, 25-31.

Using the line x tester analysis (Sing and Choudhary,1979), we studied the combining ability, gene effects and mode of inheritance of plant height and spike length, using 5 females, 3 testers and 15 F1 hybrids of wheat. The mode of inheritance of characters under study depended on the cross combination and the year of growing. In most cases the mode of inheritance was dominant. Estimation of the genetic components of variation as well as ratio of GCA/SCA showed that plant height in the first year and spike length in the second year of research were predomi­nantly controlled by additive gene action. Non-additive gene effects have been found to be more important than additive ones in the inheritance of plant height in the second year and spike length in the first year of re­search. The estimates of general combining ability (GCA) pointed out that the best general combiner for the plant height in the first year was line NS 31/96, while in the second year the best combiner was variety Fundulea 490. For the spike length the best general combiner in the both years was line NS 31/96. The line NS 31/96 was the best general combiner in this research and it can be used in wheat breeding.

Key words: wheat, combining ability, mode of inheritance, gene effects

 

 

 

Corresponding author: Biljana Gorjanović, Primary school “Dušan Jerković”, Dušana Jerkovića 1, 22320 Inđija, Serbia and Montenegro

e-mail: bgorjanovic@yahoo.com

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 33-38
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21: 633.31/.37

doi:10.2298/GENSR0501033N

Original scientific paper

 

ANALYSIS OF MORPHOLOGICAL TRAITS OF BIRD’S FOOT TREFOIL PLANTS CV. BOKOR TRANSFORMED WITH AGROBACTERIUM RHIZOGENES

Radomirka NIKOLIĆ and Nevena MITIĆ

 

Agricultural Research Institute “Serbia”, Center for Agricultural and Technological Research, 19000 Zaječar, Serbia and Montenegro

 

 

Abstract

 

Nikolić R. and N. Mitić (2005): Analysis of morphological traits of bird’s foot trefoil plants cv. bokor transformed with Agrobacte­rium rhizogenes. – Genetika, Vol. 37, No. 1, 33-38.

An efficient method for genetic transformation and shoot regen­eration was achieved in bird’s foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed T0 plants were grown in experimental field. Analysis of morphological traits and chemi­cal content in ten unintentionally chosen T0 bird’s foot trefoil plants (genotypes no. 2 and no. 5) was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype and “T” phenotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants.

Key words: Lotus corniculatus, morphological traits, transformed plants, hairy roots, A. rhizogenes, rol genes

 

 

Corresponding author: Dr Radomirka Nikolić, ARI “Srbija”, ARI “Serbia”, Center for Agricultural and Technological Research, Grljanski put b.b., 19000 Zaječar, Se rbia and Montenegro

Tel: 019/420-222, Fax: 019/421-668; e-mail: czpiti@ptt.yu

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 39-47
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21; 634.23

doi:10.2298/GENSR0501039M

Original scientific paper

 

POMOLOGICAL AND TECHNOLOGICAL CHARACTERISTICS OF COLLECTED SELECTIONS OF CHERRY PLUM PRUNUS CERASIFERA ERHR.

Rade MILETIĆ, Mihajlo ŽIKIĆ, Nevena MITIĆ, and Radomirka NIKOLIĆ

 

ARI “Serbia”, Agricultural and Technological Research Centre, 19 000 Zaječar, Serbia and Montenegro

 

 

Abstract

 

Miletić R., M. Žikić, N. Mitić, and R. Nikolić (2005): Pomologi­cal and technological characteristics of collected selections of cherry plum Prunus cerasifera Erhr. – Genetika, Vol. 37, No. 1, 39-47.

A plantation collection containing 32 genotypes selected from spontaneous populations of cherry plum Prunus cerasifera Erhr. was set up in the region of the Eastern Serbian town of Svrljig. The fruit trees budded from Prunus cerasifera seedlings and were planted at 5x4 m spacing on a mild slope of south-western aspect. This study shows the most important characteristics of the 19 selections in the collection, and the average results recorded in the 2000-2003 period. The most signifi­cant characteristics of the trees, their productivity, and fruit and stone characteristics are presented. The averge coarseness of fruits, i.e. their lenght, width and thickness, measured 25.0x 24.4x25.0 mm, while stone coarseness was 14.4x10.3x3.6 mm.The average fruit weight was 12.1 g (24.3-4.8 g), and stone weight 0.85 g (2.2-0.3 g). Depending on fruit and stone weight, the mesocarp content was 93% (96.3-90.3%). Taking into consideration the possibility of fruit exploitation for the production of biologically high-quality food, the mesocarp chemical composition was thoroughly examined. The fruits were found to have increased contents of total acids, achieving an average of 3.09% (3.44-2.60%), which was the initial objective of this selection. Total solids content was 13.5% (16.2-10.3%), total soluble solids 12.5% (14.5-9.5%) and total sugars 6.00% (11.45-3.14%). Considering these characteristics, the selections that were singled out deserve more attention in terms of preserving their biodiversity, forming a gene bank and commercial cultivation.

Key words: Cherry plum, selections, genotype

 

 

Corresponding author: Rade Miletić, ARI “Serbia”, Agricultural and Technological Research Centre, 19 000 Zaječar, Serbia and Montenegro

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 49-55
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21; 636.85

doi:10.2298/GENSR0501049T

Original scientific paper

PHENOTYPE VARIABILITY AND INHERITANCE OF LEAF SHAPE IN F1 GENERATION OF SUNFLOWER

Sreten TERZIĆ and Jovanka ATLAGIĆ

 

Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia and Montenegro

 

 

Abstract

 

Terzića S. and J. Atlagić (2005): Phenotype variability and in­heritance of leaf shape in F1 generation of sunflower. – Genetika, Vol. 37, No. 1, 49-55.

Populations of wild sunflower species were crossed with cms cultivated lines because of their high variability. Variability was deter­mined by measuring inflorescence diameter, ray flower number and the leaf length and width. The data was used for hierarchical cluster analysis in the SYSTAT 10 program and the obtained dendrogram was used to interpret divergence of used populations. Comparing 25 hybrid popula­tions with parents tested the modes of inheritance. Cluster analysis di­vided plants in to three groups. The first ones were inbred lines of culti­vated sunflower. In the middle of the cluster tree were annual wild species and the third group were perennial wild species. The mean value differ­ences in observed traits between parents were significant. All modes of inheritance were present in F1 generation. Intermediate was the most fre­quent followed by equal number of partially dominant and dominant ones and in two hybrid combinations, negative heterotic effect was scored.

Key words: sunflower, wild species, interspecies hybridisation, variability, leaf shape, inheritance

 

 

Corresponding author: Sreten Terzić, Institute for field and vegetable crops, Novi Sad.

e-mail: terzic@ifvcns.ns.ac.yu

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 57-64
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.21; 636.85

doi:10.2298/GENSR0501057G

Original scientific paper

GENE EFFECT AND COMBINING ABILITIES FOR PLANT HEIGHT AND HEAD DIAMETER IN SUNFLOWER

Sandra GVOZDENOVIĆ, Jovan JOKSIMOVIĆ, Dragan ŠKORIĆ

 

Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro

 

 

Abstract

 

Gvozdenović S., J. Joksimović, and D. Škorić (2005): Gene ef­fect and combining abilities for plant height and head diameter in sun­flower. – Genetika, Vol. 37, No. 1, 57-64.

Plant height and head diameter are important parameters which effect on a yield in sunflower. Six restorer Rf lines, three tester A lines and their 18 F1 hybrids were studied, using line x tester method. Signifi­cant differences were found between restorer lines and testers and their F1 hybrids for plant height and head diameter. Regarding the inheritance of examined characteristic, super dominance and dominance of better parent occurred, and the intermediary too. Tester line HA-48A (188.25 cm) and F1 hybrid HA-48A x RHA-SELEUS (245.10 cm) had the highest mean value for plant height, and the lowest value had line RHA-BRE-1 (105.35 cm) and hybrid L-19A x RHA-BRE-1 (147.9 cm). For head diameter, the highest mean value had line L-19A (19.02 cm) and F1 hybrids HA-48A x RHA-TR-20 and L-19A x RHA-TR-20 (24.55 cm), and the lowest line RHA-BRE-1 (13.10 cm) and hybrid HA-26A x RHA-M-72 (20.25 cm). Based on the results, the following conclusion is that lines RHA-BRE-1 for plant height and RHA-SELEUS for head diameter have the best GCA, and the best SCA have hybrids L-19A x RHA-BRE-1 for plant height and HA-26A x RHA-SELEUS for head diameter. Analyzing components of genetic variance, the nonadditive component played the main role in the inheritance of plant height and the additive of head diameter. The largest average contribution in the expression of plant height had the tester A-lines (83.17%) and for head diameter Rf lines had the greatest influence (58.13%).

Key words: sunflower, combining abilities, GCA, SCA, plant height, head diameter

 

 

Corresponding author: Sandra Gvozdenović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro, tel: +381 21 4898403,

e-mail: sandra@ifvcns.ns.ac.yu

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 65-70
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.222.5: 635.652

doi:10.2298/GENSR0501065Z

Original scientific paper

 

COMBINING ABILITIES OF INHERITING FIRST POD HEIGHT OF SOME FRENCH BEAN LINES (PHASEOLUS VULGARIS L.)

Milan ZDRAVKOVIĆ, Jasmina ZDRAVKOVIĆ, Ljiljana STANKOVIĆ, and Nenad PAVLOVIĆ

 

Agricultural Research Institute "Serbia" - Center for Vegetable Crops, 71 Karađorđeva St, 11420 Smed. Palanka, Serbia and Montenegro

 

 

Abstract

 

Zdravković M., J. Zdravković, Lj. Stanković, and N. Pavlović (2005): Combining abilities of inheriting first pod height of some french bean lines (Phaseolus vulgaris L.). – Genetika, Vol. 37, No. 1, 65-70.

First pod height is an important trait, especially for snap bean since it enables the mechanical harvesting. Cultivars with low first pod height may be cut, damaged or not harvested by mechanical harvesting. In our investigation six different snap bean genotypes were used for exami­nation of the first pod height and diallel analysis. The result was obtained by using method 2 model 1 Griffing (1956) for analysis of combining abilities. The mode of inheritance was different, as the results showed - from intermediate to super dominance, for both generations (F1 and F2). Analysis of combining abilities showed both influence of additive and dominant gene effects. Combination Šumadinka x Zora is suitable for further breeding for first pod height.

Key words: snap bean, inheritance, first pod

 

Corresponding author: Milan Zdravković, Agricultural Research Institute "Serbia" - Center for Vegetable Crops, 71 Karadjordjeva St, 11420 Smed. Palanka, Serbia and Montenegro,

e-mail: mzdravkovic@cvcsp.co.yu

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 71-76
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.827: 633.15

doi:10.2298/GENSR0501071D

Original scientific paper

 

THE EFFECT OF A HIGH SELECTION INTENSITY ON THE CHANGE OF MAIZE YIELD COMPONENTS’ ADDITIVE VARIANCE

Nebojša DELETIĆ, Slaviša STOJKOVIĆ, Vladan ĐURIĆ, Slaviša GUDŽIĆ, and Milan BIBERDŽIĆ

 

University of Pristina, Faculty of Agriculture, Lešak, Serbia and Montenegro

 

 

Abstract

 

Deletić N., S. Stojković, V. Đurić, S. Gudžić, and M. Biberdžić (2005): The effect of a high selection intensity on the change of maize yield components’ additive variance. – Genetika, Vol. 37, No. 1, 71-76.

A set of 31 SSD lines from ZP-Syn-1 C0 and 37 from ZP-Syn-1 C3 maize population was studied in this paper. After line selection and seed multiplication in 2000, the trials were carried out in 2001-2002, at Krusevac and Zemun Polje, in RCB design. After three cycles of recur­rent selection we observed a significant decrease of homozygous proge­nies' means of root and stalk lodged plants percent, plant and ear height, but also of row number per ear. The means of grain number per ear and 1000 grain mass were increased, and the differences in ear length and grain yield were not significant. After three cycles, a significant narrow­ing of additive variance happened for root and stalk lodged plant percent, ear length, and row number per ear, but this narrowing was not significant for the other traits. We also found significant values of narrow-sense heritability.

Key words: maize, yield components, additive variance, selection inten­sity

 

Corresponding author: Nebojša Deletić, University of Priština, Faculty of Agriculture, Lešak, Serbia and Montenegro

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 77-85
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 579.25

doi:10.2298/GENSR0501077S

Original scientific paper

 

ANALYSIS OF NATURAL ISOLATES OF LACTOBACILLI RESISTANT TO BACTERIOCIN NISIN

Ivana STRAHINIĆ1, Jelena BEGOVIĆ1, Đorđe FIRA1, Mihailo OSTOJIĆ2, and Ljubiša TOPISIROVIĆ1

 

1 Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11010 Belgrade,

2 Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Zemun, Serbia & Montenegro.

 

 

Abstract

 

Strahinić I., J. Begović, Đ. Fira, M. Ostojić, and Lj. Topisirović (2005): Analysis of natural isolates of Lactobacilli resistant to bacteriocin nisin. – Genetika, Vol. 37, No. 1, 77-85.

The collection of lactic acid bacteria (LAB) was made by isola­tion of microorganisms from fermented products traditionally manufac­tured in different geographical regions (high mountains, river valleys, seaside, etc). Among collected LAB, 51 isolates were identified as Lacto­bacillus sp. Results showed that all isolated lactobacilli were mesophilic strains, since they grew at 15°C and 30°C but not at 45°C. Testing the ability of isolated lactobacilli to grow in the presence of nisin revealed that Lactobacillus sp. isolates designed BGCGK4, BGHN40, BGBUK2-8, BGBUK2-7 and BGBUK2-16 were resistant to nisin. Determination of the minimal inhibitory concentrations (MIC) for nisin revealed that the most resistant isolate was Lactobacillus sp. BGCGK4. Isolate BGBUK2-16, determined as Lactobacillus paracasei subsp. paracasei, produces bacteriocin, named Bac217 and showed a resistance to 8000 IU/ml of nisin. Plasmid curing of BGBUK2-16 resulted in derivatives BGBUK2-16/K2 and BGBUK2-16/K4. Derivative BGBUK2-16/K2 retained resis­tance to Bac217 and nisin, but lost the ability to synthesise Bac217. De­rivative BGBUK2-16/K4 lost concomitantly the resistance to both Bac217 and nisin.

Key words: Lactobacillus, natural isolates, antimicrobial activity, nisin, resistance

 

Corresponding author: Ivana Strahinić, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O.Box 23, 11010 Belgrade, Serbia & Montenegro.

Telephone: +381 11 397 59 60

Fax: +381 11 397 58 08

E-mail: lab6@eunet.yu

 

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Published in „GENETIKA“ Vol. 37, No.1 (2005), pp. 87-92
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 633.15; 631.523

doi:10.2298/GENSR0501087J

Original scientific paper

 

EFFECTS OF THE SOWING DENSITY ON THE YIELD AND THE NUMBER OF GERMINATED SEEDS IN SEED MAIZE

Predrag JOVIN1, Milorad ROŠULJ1, Miodrag TOLIMIR1, and Jasmina MILENKOVIĆ2

 

1Maize Research Institute, Zemun Polje, Belgrade-Zemun,

2Agricultural Research Institute „Serbia“, Agricultural and Technological Research Centre, Zaječar, Serbia and Montenegro

 

 

Abstract

 

Jovin P., M. Rošulj, M. Tolimir, and J. Milenković (2005): Ef­fects of the sowing density on the yield and the number of germinated seeds in seed maize. – Genetika, Vol. 37, No. 1, 87-92.

The trials were carried out in the regular production of the seed maize ZP 680 at the agricultural estate “Nova Budućnost” Žarkovac dur­ing 2001 and 2002. Sowing densities (71,400, 85,500, 99,900 plants ha-1) affected varying of yields (4.01, 4.38. 4.40 t ha-1). The 1000-seed weight decreased over increased densities (305.6, 2912, 282.5 g). Germination was even over densities (95.0, 94.9, 94.7 %). A greater number of germi­nated seeds per area unit (13,122,000, 15,022,000, 15,569,000) was ob­tained in greater densities. The percentile share of fractions in the weight was uniform over all sowing densities.

Key words: sowing density, seed maize, 1000-seed weight, number of seeds per hectare, seed fraction

 

 

Corresponding author: Milorad Rošulj, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun, Serbia and Montenegro

Tel: +381-11-3756704; fax: +381-11-3756707;

e-mail: mrosulj@mrizp.co.yu

 

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