GENETIKA, Vol. 37, No.2 (2005)
Milena KIRILOVA, Rumen IVANOV, and George MILOSHEV
A NOVEL PARAMETER IN COMET ASSAY MEASUREMENTS [Abstract] [Full text]
Rade MILETIĆ, Mihajlo ŽIKIĆ, Nevena MITIĆ, and Radomirka NIKOLIĆ
POMOLOGICAL CHARACTERISTICS OF SUPERIOR SELECTIONS OF EUROPEAN FILBERT (C. AVELLANA L.) AND TURKISH HAZEL (C. COLURNA L.) [Abstract] [Full text]
Nebojša ANASTASIJEVIĆ, Mirjana OCOKOLJIĆ, and Vesna VRATUŠA
PROPERTIES AND VARIABILITY OF DOUGLAS-FIR SEEDLINGS (PSEUDOTSUGA MENZIESII (MIRB.) FRANCO) [Abstract] [Full text]
Dejana SAFTIĆ-PANKOVIĆ, Jovanka ATLAGIĆ, Tomka MILJANOVIĆ, and Nataša RADOVANOVIĆ
MORPHOLOGICAL AND MOLECULAR VARIABILITY OF HELIANTHUS GIGANTEUS L. AND HELIANTHUS MAXIMILIANI SCH. SPECIES [Abstract] [Full text] [Table]
Milan BIBERDŽIĆ, Midrag ĐORĐEVIĆ, Saša BARAĆ, Nebojša DELETIĆ, and Slaviša STOJKOVIĆ
PRODUCTIVITY OF SOME WINTER WHEAT GENOTYPES [Abstract] [Full text]
Zoran BEŠLIĆ, Slavica TODIĆ, and Vera RAKONJAC
INHERITANCE OF SOME MORPHOLOGICAL TRAITS IN HYBRIDIZATION OF GRAPEVINE CULTIVARS DRENAK CRVENI AND AFUZ-ALI [Abstract] [Full text]
Radovan MARINKOVIĆ and Ana MARJANOVIĆ-JEROMELA
ASSESSMENT OF COMPONENTS OF GENETIC VARIANCE OF MASS 1000 SEEDS IN SUNFLOWER (HELIANTHUS ANNUUS L.) [Abstract] [Full text] [Table]
S.P. SINGH, R.S. KATIYAR, S.K. RAI, S.M. TRIPATHI1, and J.P. SRIVASTVA
GENETIC DIVERGENCE AND ITS IMPLICATION IN BREEDING OF DESIRED PLANT TYPE IN CORIANDER (CORIANDRUM SATIVUM L.) [Abstract] [Full text] [Table]
Vladislava GALOVIĆ, Zorana KOTARANIN, and Srbislav DENČIĆ
IN VITRO ASSESSMENT OF WHEAT TOLERANCE TO DROUGHT [Abstract] [Full text]
Veselinka ZEČEVIĆ, Desimir KNEŽEVIĆ, Danica MIĆANOVIĆ, Milanko PAVLOVIĆ, and Dušan UROŠEVIĆ
THE INHERITANCE OF PLANT HEIGHT IN WINTER WHEAT (TRITICUM AESTIVUM L.) [Abstract] [Full text]
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 93-101
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.113.1
Original scientific paper
A NOVEL PARAMETER IN COMET ASSAY MEASUREMENTS
Milena KIRILOVA, Rumen IVANOV1, and George MILOSHEV
Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria
1 Institute für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr. 3 06466 Gatersleben, Germany
Abstract
Kirilova M., R. Ivanov, and G. Miloshev (2005): A novel parameter in comet assay measurements. – Genetika, Vol. 37, No. 2, 93-101.
Single Cell Gel Electrophoresis (SCGE) or Comet assay is a very sensitive method for assessing damages in DNA on a single cell level. It has found many applications in fields where genotoxic activity could be an issue. In environmental monitoring, health care, food industry Comet assay is used with increasing popularity. For verifying the results obtained by this method many parameters could be monitored. To that end several software packages exist. In the conditions that we are suggesting one more parameter could be measured – comet shape. We argue that this parameter could be an advantage of the Comet Assay when the way of DNA damaging needs to be predicted.
Key words: Comet Assay, apoptosis, micrococcal nuclease
Corresponding author: George Miloshev, Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria
Fax: + 359 2 723507;
E-mail: miloshev@obzor.bio21.bas.bg
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 103-111
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.2; 634.54
Original scientific paper
POMOLOGICAL CHARACTERISTICS OF SUPERIOR SELECTIONS OF EUROPEAN FILBERT (C. AVELLANA L.) AND TURKISH HAZEL (C. COLURNA L.)
Rade MILETIĆ, Mihajlo ŽIKIĆ, Nevena MITIĆ, and Radomirka NIKOLIĆ
ARI “Serbia”, Agricultural and Technological Research Centre, 19000, Zaječar, Serbia and Montenegro
Abstract
Miletić R., M. Žikić, N. Mitić, and R. Nikolić (2005): Pomological characteristics of superior selections of european filbert (C. avellana L.) and turkish hazel (C. colurna L.). – Genetika, Vol. 37, No. 2, 103-111.
Eight selections of European filbert and Turkish hazel each were singled out for their prominent characteristics in an examination in Eastern Serbia over the 1998-2003 period. Coarseness, form and weight of nuts and kernels, as well as kernel chemical content, were analysed. Yielding potential was investigated as a proportion of nuts in filbert and hazel inflorescences. Average nut coarseness of the C. avellana L. selections was 18.0x14.7x12.6 mm, and of C. colurna L. 16.3x14.0x 11.0 mm, while average kernel coarseness was 13.3x9.1x7.6 mm and 13.9x 9.7x 6.5 mm, respectively. Nut weight of the former was 1.01-1.80 g, and of the latter 1.00-1.75 g, while kernel weight ranged 0.30-0.79 g, and 0.31-0.65 g, respectively. The respective kernel contents were 27.9-46.2%, and 30.7-40.8 %. The occurrence of shriveled nuts was minimal, appearing in six selections of C. avellana (0.8-3.1%), and five selections of C. colurna (1.4-5.7%). The pest Balaninus nucum was observed in only three selections of C. colurna L., ranging from 1.0 to 1.6 %. Oil content in the nuts of the selected C. avellana L. trees was 44.6-49.9 %, and 48.6-54.4 % in those of C. colurna L. Crude proteins amounted to 12.3-10.8 % and 11.7-10.4 %, and mineral matter content to 2.8-2.4% and 2.6-2.0 %, respectively. The selections of European filbert were found to have 4-6 nuts to an inflorescence. Predominating were two (29.4 %), one (26.7 %) and three (24.5 %) nuts to a single inflorescence. C. colurna L. inflorescences were found to contain 8-13 nuts. Most inflorescences had five (26.6 %), four (21.5 %) and six nuts (20.3 %). Selections 1, 5, 6 and 3 of C. avellana were found to be especially outstanding regarding most parameters, as well as selections 7, 3, 2 and 8 of C. colurna L.
Key words: European filbert, Turkish hazel, selection, nut, yielding potential
Corresponding author: Rade Miletić, ARI “Serbia”, Agricultural and Technological Research Centre, 19000 Zaječar, Serbia and Montenegro
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 113-119
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21
Original scientific paper
PROPERTIES AND VARIABILITY OF DOUGLAS-FIR SEEDLINGS (PSEUDOTSUGA MENZIESII (MIRB.) FRANCO)
Nebojša ANASTASIJEVIĆ, Mirjana OCOKOLJIĆ, and Vesna VRATUŠA
Department for Green Area Establishment and Maintenance, Faculty of Forestry, 11000 Belgrade, Serbia and Montenegro
Abstract
Anastasijević N., M. Ocokoljić, and V. Vratuša (2005): Properties and variability of douglas-fir seedlings (Pseudotsuga menziesii (Mirb.) Franco). – Genetika, Vol. 37, No. 2, 113-119.
Based on the comparative morphophysiological analysis of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings, three half-sib lines from Quercetum farneto-cerris s.l. site, and three half-sib lines from Saliceto-Populetum s.l. site, the data on seedling characteristics and variability of this cultivated species are discussed. The paper also ascertains the variability of seedling properties that affect mother tree selection and production technology of plant material of desired characteristics for the application in forestry and landscape architecture and horticulture.
Key words: Douglas-fir, Pseudotsuga menziesii, variability, properties
Corresponding author: Nebojša Anastasijević, Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade,
tel: 381-11-3553122
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 121-130
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 577.21; 636.85
Original scientific paper
MORPHOLOGICAL AND MOLECULAR VARIABILITY OF HELIANTHUS GIGANTEUS L. AND HELIANTHUS MAXIMILIANI SCH. SPECIES
Dejana SAFTIĆ-PANKOVIĆ, Jovanka ATLAGIĆ, Tomka MILJANOVIĆ, and Nataša RADOVANOVIĆ
Institute of Field and Vegetable Crops, 21000 Novi Sad, Maksima Gorkog 30, Serbia and Montenegro
Abstract
Saftić-Panković D., J. Atlagić, T. Miljanović, and N. Radovano vić (2005): Morphological and molecular variability of Helianthus giganteus L. and Helianthus maximiliani Sch. species. – Genetika, Vol. 37, No. 1, 121-130.
Genus Helianthus consists of 49 species. Two species H. giganteus L. and H. maximiliani Sch., distributed and collected in North America, were investigated. In order to determine morphological variability in/between these two species, fifteen populations of each species were used. Thirty traits were measured on five plants per species, grown in the same conditions in the wild species nursery at Rimski Šančevi. According to the investigated morphological traits, three species of H. giganteus were closer to H. maximiliani populations, which possibly indicates the existence of a new intraspecies taxon in H. giganteus. In order to test this hypothesis molecular variability of the same populations-species, was also investigated. The polymorphism of genomic DNA, that was isolated from frozen leaves, was investigated by microsatellites, recently shown to be the most powerfull for the analysis of molecular genetic variability in genus Helianthus. Obtained results confirm the high variability between examined populations. Dendrograms constructed by cluster analysis of examined morphological traits and molecular markers are discussed.
Key words: sunflower wild species, intraspecies variability, microsatellites
Corresponding author: Dejana Saftić-Panković, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Phone: 381 21 4898 416; fax 381 21 413 833; e-mail: dejanapa@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 131-136
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21: 633.11
Original scientific paper
PRODUCTIVITY OF SOME WINTER WHEAT GENOTYPES
Milan BIBERDŽIĆ1, Midrag ĐORĐEVIĆ1, Saša BARAĆ1, Nebojša DELETIĆ1, and Slaviša STOJKOVIĆ2
1University of Priština, Faculty of Agriculture, Lešak;
2 Institute of Agricultural Research “Serbia”, Centre for Small Grains, Kragujevac, Serbia and Montenegro
Abstract
Biberdžić M., M. Đorđević, S. Barać, N. Deletić, and S. Stojković (2005): Naziv rada. – Genetika, Vol. 37, No. 2, 131-136.
Yields given by different genotypes are affected by many factors, but mostly by genotype, climatic conditions and applied growing practice. Therefore, the aim of this study was to establish productivity of many winter wheat genotypes in southern Serbia, through macro-trials. The results showed the difference of grain yield mean between the investigated seasons, and that difference amounted about 1300 kg/ha. The average yield was the highest in cultivars Toplica, Stamena, Evropa, NS-rana 5, and Renesansa. The lowest grain yield was given by cultivars Tina, Sara, Sreca, and Mina.
Key words: winter wheat, productivity, genotype
Corresponding author: Milan Biberdžić, University of Priština, Faculty of Agriculture, Lešak, Serbia and Montenegro
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 137-144
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 634.8.05
Original scientific paper
INHERITANCE OF SOME MORPHOLOGICAL TRAITS IN HYBRIDIZATION OF GRAPEVINE CULTIVARS DRENAK CRVENI AND AFUZ-ALI
Zoran BEŠLIĆ, Slavica TODIĆ, and Vera RAKONJAC
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Zemun, Serbia and Montenegro
Abstract
Bešlić Z., S. Todić, and V. Rakonjac (2005): Inheritance of some morphological traits in hybridization of grapevine cultivars Drenak crveni and Afuz-Ali. – Genetika, Vol. 37, No. 2, 137-144.
The variability and mode of inheritance of young shoot tip, leaf, grape and berry morphological characteristics were investigated in F1 generation obtained by crossing of Drenak crveni and Afuz-ali. Based on seedlings description, considering investigated characteristics, grouping was done by applying O.I.V. method. The conclusions about inheritance of characteristics were obtained based on results of χ2 test. The consider able variability was expressed in hybrid population. For properties of leaf characteristics (largeness, shape, dividity, and incisions), exception from typical monogenic ratio was determined. The monohybrid inheritance was determined in density of leaf hairs and downs. The monohybrid mode of inheritance was not confirmed in grape characteristics (largeness, com pactness and stem length) and berry characteristics (largeness, shape and taste), while skin color is monogenic property.
Key words: variability, monogenic inheritance, morphological charac teristics, F1 generation, grapevine
Corresponding author: Zoran Bešlić, Faculty of Agriculture, Nemanjina 6, 11080 Zemun, Serbia and Montenegro e-mail: zbeslic@agrifaculty.bg.ac.yu
Phone. 2615-315 / 317
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 145-153
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 636.85
Original scientific paper
ASSESSMENT OF COMPONENTS OF GENETIC VARIANCE OF MASS 1000 SEEDS IN SUNFLOWER (HELIANTHUS ANNUUS L.)
Radovan MARINKOVIĆ and Ana MARJANOVIĆ-JEROMELA
Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Abstract
Marinković R. and A. Marjanović-Jeromela (2005): Assessment of components of genetic variance of mass 1000 seeds in sunflower (Helianthus annuus L.). – Genetika, Vol. 37, No. 2, 145-153.
Ten F1 hybrids obtained by crossing five sunflower inbred lines were used to analyze the impact of genes with additive and dominant effects and their interactions on the inheritance of mass 1000 seeds. The linkage among the expected progeny means was tested using the scaling tests method (Mather, 1949), while the estimates of gene effects and mode of inheritance were made by generation mean analysis (Mather and Jinks, 1982). The additive-dominant model was not proved adequate for all crosses in both years of study. It was adequate in crosses C1, C2, C3 and C5 in the first year and in crosses C3, C8, C9 and C10 in the second year of study. Besides the main gene effects (additive and dominant), epistatic gene effects were also of large importance in the inheritance of this trait. Duplicate epistasis between dominant decreasers was found in C1, C4, C5, C6 and C8 in the first year and in crosses C4, C9 and C10 in the second year of study. Complementary epistasis between dominant decreasers was found in cross C10 in the first year and duplicate epistasis between dominant increasers in cross C5 in the second year of study.
Key words: scaling tests method, additive-dominant model, inheritance, duplicate epistasis, complementary epistasis
Corresponding author: Radovan Marinkovic, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
e-mail: radomar@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 155-163
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22
Original scientific paper
GENETIC DIVERGENCE AND ITS IMPLICATION IN BREEDING OF DESIRED PLANT TYPE IN CORIANDER (CORIANDRUM SATIVUM L.)
S.P. SINGH, R.S. KATIYAR, S.K. RAI, S.M. TRIPATHI1, and J.P. SRIVASTVA1
Division of Genetics and Plant Breeding, National Botanical Research Institute Lucknow-226001, U.P.,
1 Division of Vegetable Science, Chandra Shekhar Azad University of Agriculture and Technology Kanpur- 208024, India
Abstract
SINGH S.P., R.S. KATIYAR, S.K. RAI, S.M. TRIPATHI, and J.P. SRIVASTVA (2005): Genetic divergence and its implication in breeding of desired plant type in coriander (Coriandrum sativum L.). – Genetika, Vol. 37, No. 2, 155-163.
Seventy germplasm lines of coriander (Coriandrum sativum L.) of diverse eco-geographical origin were undertaken in present investigation to determine the genetic divergence following multivariate and canonical analysis for seed yield and its 9 component traits. The 70 genotypes were grouped into 9 clusters depending upon the genetic architecture of genotypes and characters uniformity and confirmed by canonical analysis. Seventy percent of total genotypes (49/70) were grouped in 4 clusters (V, VI, VIII and IX), while apparent diversity was noticed for 30 percent genotypes (21/70) that diverged into 5 clusters (I, II, III, IV, and VII). The maximum inter cluster distance was between I and IV (96.20) followed by III and IV (91.13) and I and VII (87.15). The cluster VI was very unique having genotypes of high mean values for most of the component traits. The cluster VII had highest seeds/umbel (35.3 ± 2.24), and leaves/plant (12.93 ± 0.55), earliest flowering (65.05 ± 1.30) and moderately high mean values for other characters. Considering high mean and inter cluster distance breeding plan has been discussed to select desirable plant types.
Key words: Coriandrum sativum L., germplasm, multivariate analysis, Canonical vector, clusters
Corresponding author: Dr. S.P. Singh, Division of Genetics and Plant Breeding, National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India.
Tel: 91-522-2205842; Fax: 91-2205836; e-mail: sant_psingh@yahoo.co.in
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 165-171
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22; 633.11
Original scientific paper
IN VITRO ASSESSMENT OF WHEAT TOLERANCE TO DROUGHT
Vladislava GALOVIĆ, Zorana KOTARANIN, and Srbislav DENČIĆ
Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Abstract
Galović V., Z. Kotaranin, and S. Denčić (2005): In vitro assessment of wheat tolerance to drought. – Genetika, Vol. 37, No. 2, 165-171.
Analyzed in this paper were the in vitro effects of drought stress in 13 genotypes of winter wheat, one genotype of spring wheat, and three Triticale genotypes of different geographic origin. Callus tissue was induced from immature zygotic embryoes (10-15 days after pollination) on a modified MS nutrient medium. After two weeks, callus tissue was transplanted onto the same medium enriched with 5% high-molecular polyethylene glycol (PEG 6000), which was used as the stress agent to produce the effect of drought chemically. A control group of calluses was grown on an identical medium but without PEG. After four weeks of growing calluses on these mediums, we assessed callus mass survival ability of the genotypes before the transplantation as well as percentage reduction of callus fresh weight after the transplantation onto the nutrient medium with 5% PEG. Statistically significant differences were found among the genotypes in their response to the induced stress. The best survival ability before the transplantation was found in the genotype Mexico120 (83%), while the lowest was recorded in Slavija (11.3%). Culture growing under stress conditions significantly reduced callus fresh weight in all of the genotypes. The lowest decrease of the callus mass relative to control was recorded in Rozofskaja (14.4%) and the highest in Miranovska (58.4%), indicating the genotypes' tolerance levels towards drought stress.
Key words: whear, drought, embryo culture
Corresponding author: Vladislava Galović, Institute of Field and Vegetable Crops, Novi Sad, Maksima Gorkog 30, Serbia and Montenegro
e-mail: vgalovic@yahoo.com
Published in „GENETIKA“ Vol. 37, No.2 (2005), pp. 173-179
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.827:631.633.11
Original scientific paper
THE INHERITANCE OF PLANT HEIGHT IN WINTER WHEAT (TRITICUM AESTIVUM L.)
Veselinka ZEČEVIĆ, Desimir KNEŽEVIĆ, Danica MIĆANOVIĆ, Milanko PAVLOVIĆ, and Dušan UROŠEVIĆ
Agriculture Research Institute SERBIA, Center for Small Grains Kragujevac, 34000 Kragujevac, Serbia and Montenegro
Abstract
Zečević V., D. Knežević, D. Mićanović, M. Pavlović, and D. Urošević (2005): The inheritance of plant height in winter wheat (Triticum aestivum L.). – Genetika, Vol. 37, No. 2, 173-179.
Four winter wheat varieties (Srbijanka, Partizanka, KG-56 and PKB-111) have been selected for diallel crossing in other to study the mode of inheritance, gene effect and genetic variance components for the plant height in F2 generation. Sixty plants of parents and F2 generation were used for analysis. The mode of inheritance was done on the basis of the significance of components of genetic variance and the regression analysis. The inheritance of plant height in the most crossing combinations was superdominance. The combining ability analysis was found to be highly significant, which means both additive and non-additive type of gene actions. The best general combining ability manifested KG-56 variety, and the best specific combining ability have shown hybrids KG-56 x PKB-111, Srbijanka x PKB-111 and Partizanka x KG-56. The genetic variance components, average degree of dominance and regression line indicated superdominance in the inheritance of plant height. The dominant alleles frequency was higher than recessive alleles frequency, which confirmed the ratio of dominant/recessive alleles.
Key words: diallel analysis, inheritance, plant height, winter wheat
Corresponding author: Veselinka Zečević, Agriculture Research Institute SERBIA, Center for Small Grains Kragujevac, S. Kovačevića 31, 34000 Kragujevac, Serbia and Montenegro
tel.: 381-34-333-046; fax: 381-34-304-100; e-mail: joca@knez.uis.kg.ac.yu