GENETIKA, Vol. 37, No.3 (2005)
Nevena NAGL, Ivan ATANASOV, Krasimir RUSANOV, Svetlana PAUNOVIĆ, Lazar KOVAČEV, and Atanas ATANASOV
ADVANCES IN DEVELOPMENT OF TRANSGENIC RESISTANCE TO BEET NECROTIC YELLOW VEIN VIRUS (BNYVV) IN SUGAR BEET [Abstract] [Full text]
Dragan NIKOLIĆ, Vera RAKONJAC, Momčilo MILUTINOVIĆ, and Milica FOTIRIĆ
GENETIC DIVERGENCE OF OBLAČINSKA SOUR CHERRY (PRUNUS CERASUS L.) CLONES [Abstract] [Full text] [Table1] [Table2]
Vera RAKONJAC
CORRELATIVE RELATION OF YIELD AND FRUIT QUALITY WITH SOME PHENOLOGICAL PHASES IN PEACH [Abstract] [Full text]
Jovan JOKSIMOVIĆ, Jovanka ATLAGIĆ, Vladimir MIKLIČ, Nenad DUŠANIĆ, and Zvonimir SAKAČ
INTERRELATIONSHIP OF POLLINATION CONDITIONS, FERTILIZATION AND SUNFLOWER SEED YIELD [Abstract] [Full text]
Vladislava GALOVIĆ, Srbislav DENČIĆ, and Dražen JELOVAC
DETERMINATION OF POLYMORPHISM OF MICROSATELLITE PRIMERS IN HEXAPLOID WHEAT [Abstract] [Full text]
Slađana MARIĆ, Radovan BOŠKOVIĆ, Žarko TEŠOVIĆ, and Milan LUKIĆ
GENETICAL POLYMORPHISM OF ACC SYNTHASE AND ACC OXIDASE IN APPLE SELECTIONS BRED IN ČAČAK [Abstract] [Full text]
Iva BAUER, Snežana MLADENOVIĆ DRINIĆ, Milomir FILIPOVIĆ, and Kosana KONSTANTINOV
GENETIC CHARACTERIZATION OF EARLY MATURING MAIZE HYBRIDS (ZEA MAYS L.) OBTAINED BY PROTEIN AND RAPD MARKERS [Abstract] [Full text]
Milisav STOJAKOVIĆ, Goran BEKAVAC, and Nenad VASIĆ
B73 AND RELATED INBRED LINES IN MAIZE BREEDING [Abstract] [Full text
Nada HLADNI, Dragan ŠKORIĆ, and Marija KRALJEVIĆ-BALALIĆ
HETEROSIS FOR SEED YIELD AND YIELD COMPONENTS IN SUNFLOWER [Abstract] [Full text]
Milomirka MADIĆ, Aleksandar PAUNOVIĆ, Dragan ĐUROVIĆ, Marija KRALJEVIĆ-BALALIĆ, and Desimir KNEŽEVIĆ
THE ANALYSIS OF GENE EFFECT IN THE INHERITANCE OF KERNEL NUMBER PER SPIKE IN BARLEY HYBRID [Abstract] [Full text]
Marija
GUĆ-ŠĆEKIĆ1,3, Marina ĐURIŠIĆ3, Dragan ĐOKIĆ3,
Dragana VUJIĆ2,3,Ivan MILOVIĆ2,3, Slaviša DJURIČIĆ3, Danijela
RADIVOJEVIĆ3, Tanja LALIĆ3, and Milena DJURIĆ2,3
PROGNOSTIC VALUE OF CLINICAL, GENETIC AND CYTOGENETIC FINDINGS IN
NEUROBLASTOMA PATIENTS FROM SERBIA AND MONTENEGRO [Abstract] [Full text]
[Table]
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 181-189
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 633.63
Original scientific paper
ADVANCES IN DEVELOPMENT OF TRANSGENIC RESISTANCE TO BEET NECROTIC YELLOW VEIN VIRUS (BNYVV) IN SUGAR BEET
Nevena NAGL1, Ivan ATANASOV3, Krasimir RUSANOV3, Svetlana PAUNOVIĆ2, Lazar KOVAČEV1, and Atanas ATANASOV3
1 Institute of field and vegetable crops, Maksima Gorkog 30, Novi Sad,
2 Center for fruit growing and viticulture, Kralja Petra 1/9, Čačak, Serbia and Montenegro
3 AgroBioInstitute, Bul. D. Tsankov 8, Sofia, Bulgaria
Abstract
Nagl N., I. Atanasov, K. Rusanov, S. Paunović, L. Kovačev, and A. Atanasov (2005): Advances in development of transgenic resistance to beet necrotic yellow vein virus (bnyvv) in sugar beet. – Genetika, Vol. 37, No. 3, 181-189.
Fragments of viral cDNA containing the coat protein gene of beet necrotic yellow vein virus were cloned in plant transformation vector pCAMBIA3301M with the bar gene as selectable marker. Vector pC3301MCPL carrying coat protein gene with leader sequence, and pC3301MCPS with coat protein gene, were used in Agrobacterium - mediated transformation of sugar beet. The transformation method used was based on the fact that sugar beet develops axillary shoots in in vitro conditions, when placed on media with citokinins. Since this ability is not genotype or ploidy dependant it is widely used for sugar beet vegetative multiplication. Sterile seedlings, with removed cotyledons and lower half of hypocotyl, were used as starting material. After transformation explants were put on micropropagation medium with cephotaxime and phosphinotricyn (ppt), where axillary shoots started to develop. Since concentration of ppt was not selective enough, after two subcultivations it was increased twofold. Only one sample, transformed with pC3301MCPS preserved morphogenetic potential for micropropagatio, and it was tested for presence of CPS fragment and bar gene by PCR with specific primers.
Key words: rhizomania, coat protein, gluphosinate amonium, Beta vulgaris L.
Abbreviations: CAMBIA - Center for application of molecular biology in agriculture, Australia
Corresponding author: Nagl Nevena, Institute of field and vegetable crops, Maksima Gorkog 30, Novi Sad, Serbia and Montenegro, Tel. ++381 21 4898 327, Fax: ++381 21 4898 333
E-mail: nagl@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 191-198
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 634.23
Original scientific paper
GENETIC DIVERGENCE OF OBLAČINSKA SOUR CHERRY (PRUNUS CERASUS L.) CLONES
Dragan NIKOLIĆ, Vera RAKONJAC, Momčilo MILUTINOVIĆ, and Milica FOTIRIĆ
Faculty of Agriculture, 11080 Belgrade-Zemun, Serbia and Montenegro
Abstract
Nikolić D., V. Rakonjac, M. Milutinović, and M. Fotirić (2005): Genetic divergence of oblačinska sour cherry (Prunus cerasus L.) clones. – Genetika, Vol. 37, No. 3, 191-198.
Based on examination of 10 important pomologic and technologic properties, genetic divergence of 10 selected Oblačinska sour cherry clones was established. The genetic divergence between the analyzed clones was determined using the hierarchical cluster analysis. The UPGA method was used and the Euclidean distance in order to determine the difference between the groups. Four similar clone groups were obtained on the dendrogram. The objective of clone differentiation was primarily yield, although other properties were taken into account as well. As the most yielded clones for the production, that can be recommended, were clone D8 or clone D4 that are genetically very similar, and clone D3.
Key words: Oblačinska sour cherry, clone, cluster analysis
Corresponding author: Dragan Nikolić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia and Montenegro, e-mail: nikolicd@agrifaculty.bg.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 199-207
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 634.25
Original scientific paper
CORRELATIVE RELATION OF YIELD AND FRUIT QUALITY WITH SOME PHENOLOGICAL PHASES IN PEACH
Vera RAKONJAC
Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia and Montenegro
Abstract
Rakonjac V. (2005): Correlative relation of yield and fruit quality with some phenological phases in peach. – Genetika, Vol. 37, No. 3, 199-207.
Ripening time of peach cultivars that are grown up in our country is extended over a time period of almost four months. In addition to that, one of more important objectives of the peach breeding is creation of the cultivars having either very early or very late ripening time. Our so far experience is that the peach cultivars which ripening time is in the extremes is of lower fruit quality. The aim of this study, therefore, was to determine relationship and the nature of the dependence between flowering time or ripening time from one side and yield, yield components (initial and final fruit set, fruit weight and stone weight) and chemical composition of fruit (content of total dry matter, content of soluble solids, content of total, invert and reduced sugar and content of total acid) on the other side. Twenty peach cultivars each having a different ripening time were using as material. The relationship between characteristics was determined on the basis of values of genetic and phenotypic correlation coefficients. Genetic and phenotypic coefficients of correlation were calculated based on the results of monofactorial analysis of covariance where the years considered as replications. It was confirmed that flowering time was positive genetic and phenotypic correlated with initial fruit set and with final fruit set. The existence of significant genetic and phenotypic correlation between ripening time and fruit weight, between ripening time and dry matter content and between ripening time and soluble solid content represented difficulty in creation of new peach cultivars of early ripening time and improved fruit quality.
Key words: peach, yield, fruit quality, phonological phases, genetic and phenotypic coefficients of correlation
Corresponding author: Vera Rakonjac, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia and Montenegro, e-mail: verak@agrifaculty.bg.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 209-215
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.21; 636.85
Original scientific paper
INTERRELATIONSHIP OF POLLINATION CONDITIONS, FERTILIZATION AND SUNFLOWER SEED YIELD
Jovan JOKSIMOVIĆ, Jovanka ATLAGIĆ, Vladimir MIKLIČ, Nenad DUŠANIĆ, and Zvonimir SAKAČ
Institute of Field and Vegetable Crops, M. Gorkog 30, Novi Sad, Serbia and Montenegro
Abstract
Joksimović J., J. Atlagić, V. Miklič, N. Dušanić, and Z. Sakač (2005): Interrelationship of pollination conditions, fertilization and sunflower seed yield. – Genetika, Vol. 37, No. 3, 209-215.
Four commercially important sunflower hybrids (NS-H-45, NS-H-111, NS-H-702 and Velja) and their parental components (Ha-74B, Ha-98B, CMS-3-8B, Ha-26B, RHA-583, RHA-R-Pl-2/1 and RHA-113N) were used over a period of two years to study the following traits: disk flower corolla length, nectar content, pollen viability, bee visitation, fertilization percentage and seed yield. Relations among the traits were determined by path coefficient analysis. The simple correlation coefficients showed that fertilization percentage and bee visitation had a highly significant influence on seed yield. The corolla length had a positive effect on nectar content, while nectar content had a significant negative influence on pollen viability. The highest significant direct influence on seed yield was that of fertilization percentage, while the effect on nectar content on seed yield was negative but not significant. The coefficient of determination was 0.8071.
Key words: sunflower, corolla length, nectar content, pollen viability, bee visitation, fertilization percentage, seed yield
Corresponding author: Jovan Joksimović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Tel: 381 021 4898 404; e-mail: jovanj@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 217-223
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22; 633.11
Original scientific paper
DETERMINATION OF POLYMORPHISM OF MICROSATELLITE PRIMERS IN HEXAPLOID WHEAT
Vladislava GALOVIĆ1, Srbislav DENČIĆ1, and Dražen JELOVAC2
1 Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad,
2 Maize Research Institute “Zemun polje”, Slobodana Bajića 1, 11185 Belgrade- Zemun, Serbia and Montenegro
Abstract
Galović V., S. Denčić, and D. Jelovac (2005): Determination of polymorphism of microsatellite primers in hexaploid wheat. – Genetika, Vol. 37, No. 3, 217-223.
The objective of this paper was to examine the functionality of two microsatellite primers as their polymorphism levels were determined for select Novi Sad wheat genotypes. Chosen as representatives of Gatersleben wheat microsatellites (GWM) were two sets of microsatellite primers, GWM165 and GWM539, which had been described according to Roder et al. (1998a; 1998b). Twenty five wheat genotypes from the World Collection of the Institute of Field and Vegetable Crops in Novi Sad were used in the study. Genomic DNA was isolated from the plant materials using a modification of the Plaschke et al. (1995) method. PCR amplification of the desired fragments was carried out in a volume of 30 ul (Eppendorf thermocycler) according to Röder et al. (1998b). The PAGE conditions were implemented according to Galović et al. (2004). The GWM539 set, with six different alleles, showed a higher level of polymorphism than GWM165, in which three different alleles were detected for the locus concerned.
Key words: wheat, microsatellites, polymorphism
Corresponding author: Vladislava Galović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
e-mail: galovic@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 225-233
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22; 634.11
Original scientific paper
GENETICAL POLYMORPHISM OF ACC SYNTHASE AND ACC OXIDASE IN APPLE SELECTIONS BRED IN ČAČAK
Slađana MARIĆ1, Radovan BOŠKOVIĆ2, Žarko TEŠOVIĆ1, and Milan LUKIĆ1
1 ARI 'Serbia’, Fruit and Grape Research Centre, 32000 Čačak,
2 The Institute for Molecular Biology and Genetic Engineering, 11001 Belgrade,
Serbia and Montenegro
Abstract
Marić S., R. Bošković, Ž. Tešović, and M. Lukić (2005): Genetical polymorphism of ACC synthase and ACC oxidase in apple selections bred in Čačak. – Genetika, Vol. 37, No. 3, 225-233.
The work on breeding new apple cultivars, of improved quality and longer storage life has been going on for a long time at the Fruit and Grape Research Centre in Čačak. As a result nine promising apple selections, that show the range of fruit storage capabality (J/1/7, J/1/20, J/2/12, J/2/14, J/11/31, J/54/53/59, J/60/7/63, Šumatovka 1 O.P. and Šumatovka 2 O.P.), were singled out. Fruit ripening is genetically programmed, complex physiological process with the important role of plant hormone ethylene. Allelic polymorphism of the genes encoding ACC synthase and ACC oxidase, enzymes on ethylene biosynthetic pathway, was studied in promising apple selections and compared to their storage life. Polymorphism was detected by the polymerase chain reaction (PCR method) and restriction analysis with 6 restriction enzymes. Two alleles of the gene encoding ACC synthase (ACS1-1 and ACS1-2), three alleles of the ACC oxidase gene (a, b and n) were identified and a positive test for early seedling selection, the fruits of which will be characterized by long storage life, was indicated.
Key words: apple, ethylene, ACC synthase, ACC oxidase, polymorphism, fruit ripening
Corresponding author: Slađana Marić, Fruit and Grape Research Centre, Kralja Petra I 9, 32000 Čačak, Serbia and Montenegro
tel. +381 32 221375, 221413
e-mail: nidzovicsladja@yahoo.com, centarca@eunet.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 235-243
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22; 633.15
Original scientific paper
GENETIC CHARACTERIZATION OF EARLY MATURING MAIZE HYBRIDS (ZEA MAYS L.) OBTAINED BY PROTEIN AND RAPD MARKERS
Iva BAUER, Snežana MLADENOVIĆ DRINIĆ, Milomir FILIPOVIĆ, and Kosana KONSTANTINOV
Maize Research Institute „Zemun Polje“, S. Bajića 1a, 11185 Zemun Polje, Belgrade, Serbia and Montenegro
Abstract
Bauer I., S. Mladenović Drinić, M. Filipović, and K. Konstantinov (2005): Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers. – Genetika, Vol. 37, No. 3, 235-243.
Knowledge of maize germplasm genetic diversity is important for planning breeding programmes, germplasm conservation per se etc. Genetic variability of maize hybrids grown in the fields is also very important because genetic uniformity implies risks of genetic vulnerability to stress factors and can cause great losts in yield. Early maturing maize hybrids are characterized by shorter vegetation period and they are grown in areas with shorter vegetation season. Because of different climatic conditions in these areas lines and hybrids are developed with different features in respect to drought resistance and disease resistance. The objective of our study was to characterize set of early maturing maize hybrids with protein and RAPD markers and to compare this clasification with their pedigree information. RAPD markers gave significantly higher rate of polymorphism than protein markers. Better corelation was found among pedigree information and protein markers.
Key words: maize, protein markers, RAPD
Corresponding author: Iva Bauer, Maize Research Institute „Zemun Polje“, S.Bajića 1a, 11185 Zemun Polje, Belgrade, Serbia and Montenegro
Phone: +381 11 3756 704
e-mail: ibauer@mrizp.co.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 245-252
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.222.5; 633.15
Original scientific paper
B73 AND RELATED INBRED LINES IN MAIZE BREEDING
Milisav STOJAKOVIĆ, Goran BEKAVAC, and Nenad VASIĆ
Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Abstract
Stojaković M., G. Bekavac, and N. Vasić (2005): B73 and related inbred lines in maize breeding. – Genetika, Vol. 37, No. 3, 245-252.
Inbred lines B73 and Mo17 or some versions thereof are the most commonly used parental pair in the development of medium late and late maize hybrids in Serbia and Montenegro. Because of the ever-increasing importance of line B73 in maize hybrid production, we chose several B73-type lines and a few unrelated lines and crossed them. Using the pedigree method, progenies were developed up to the S6 generation. The grain yield potential of test crosses with Mo17 inbred tester, as well as ear length, number of grain rows per ear and 1,000-grain mass of lines per-se were tested. Among the new inbred lines related to B73, line 260277/2 distinguished itself by a high potential for grain yield when crossed with Mo17. Inbred lines 260465/1, 260362/1, 260747/4, 260357/13, 260151/2 and 260156/2 had a significantly longer ear than the mean value of all progenies. Compared with progeny mean, lines 260341/7, 260317/4, 260277/2 and 260187/2 had significantly more grain rows per ear, while 260362/1, 260130/5, 260277/2, 260151/2 and 260187/2 had a significantly larger 1,000-grain mass.
Key words: inbred line, B73, grain yield, 1,000-grain mass, ear length, number of rows
Corresponding author: Milisav Stojaković, Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
Tel: 38121 4898-286
e-mail: mstojak@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 253-260
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.222.7; 636.85
Original scientific paper
HETEROSIS FOR SEED YIELD AND YIELD COMPONENTS IN SUNFLOWER
Nada HLADNI1, Dragan ŠKORIĆ1, and Marija KRALJEVIĆ-BALALIĆ2
1 Institute of Field and Vegetable Crops, 21000 Novi Sad,
2 Faculty of Agriculture, 21000 Novi Sad, Serbia and Montenegro
Abstract
Hladni N., D. Škorić, and M. Kraljević-Balalić (2005): Heterosis for seed yield and yield components in sunflower. – Genetika, Vol. 37, No. 3, 253-260.
The development of new high-yielding and stable sunflower hybrids based on interspecific hybridization requires information on the heterotic effects for agronomically important traits in the F1 generation. Heterotic effects for seed yield, plant height and head diameter were studied in interspecific sunflower hybrids developed by the line x tester method. The female inbred lines were developed by interspecific hybridization, while the male restorer inbreds with good combining abilities were used as testers in the form of fertility restorers. F1 hybrids were obtained by crossing each tester with each female inbred. The inbred lines and their F1 hybrids differed significantly in their mean values of the traits under study. Heterosis values for seed yield per plant were positive and highly significant relative to both the parental mean (98.4-274.1%) and the better parent (54.8-223.2%). Significantly less heterosis was recorded in the case of plant height relative to parental mean (19.0-66.0%) and better parent (-3.9-51.6%). With head diameter, the heterotic effect ranged from 19.0 to 55.6% (parental mean) and from 7.8 to 36.6% (better parent). The results of this study may be used for the development of new high-yielding and stable sunflower hybrids based on interspecific hybridization.
Key words: sunflower, heterosis, seed yield, seed yield components
Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro,
tel: 381 21 48-98-411, faks; 381 21 413 833; e-mail nadah@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 37, No.3 (2005), pp. 261-269
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.3; 633.16
Original scientific paper
THE ANALYSIS OF GENE EFFECT IN THE INHERITANCE OF KERNEL NUMBER PER SPIKE IN BARLEY HYBRID
Milomirka MADIĆ1, Aleksandar PAUNOVIĆ1, Dragan ĐUROVIĆ1, Marija KRALJEVIĆ-BALALIĆ2, and Desimir KNEŽEVIĆ3
1Faculty of Agronomy, Cara Dušana 34, 32000 Čačak,
2Faculty of Agriculture, 21000 Novi Sad,
3Center for Small Grains Kragujevac, Serbia and Montenenegro
Abstract
Madić M., A. Paunović, D. Đurović, M. Kraljević-Balalić, and D. Knežević (2005): The analysis of gene effect in the inheritance of kernel number per spike in barley hybrid. – Genetika, Vol. 37, No. 3, 261-269.
In diallel crossing, excluding reciprocal ones, five divergent genotypes of barley (Vada, Đerdap, NS-293, Jagodinac and Sladoran) were included with the mode of inheritance and gene effect studied using the regression analysis of variance and co-variance for the number of kernels per spike. The absence of inter-allele interaction established using the regression analysis VrWr and WrW’ resulted from the use of the mode selected. In the inheritance of the number of kernels per spike, the regression pointed at dominant gene effect as well as at the unevenly distributed dominant and recessive genes in the genotypes selected. While Jagodinac, Vada and NS-293 had the highest number of dominant, Đerdap and Sladoran had the highest one of the recessive genes for the number of kernels per spike. Therefore, in the two-rowed genotypes, the higher number of dominant genes was, the higher number of kernels per spike was, and conversely, the higher number of the recessive genes was, the lower number of kernels per spike was.
Key words: barley, the number of kernels, gene effect, inheritance, heritability
Corresponding author: Milomirka Madić, Faculty of Agronomy, Cara Dušana 34, 32000 Čačak, Serbia and Montenegro
Phone: +381 32 303 400; fax: +381 32 345 264;
e-mail: mmadic@tfc.kg.ac.yu
Published in „GENETIKA“ Vol.
37, No.3 (2005), pp. 271-279
© 2005 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633
doi:10.2298/GENSR0503271G
Original scientific paper
Marija
GUĆ-ŠĆEKIĆ1,3, Marina ĐURIŠIĆ3,
Dragan ĐOKIĆ3, Dragana VUJIĆ2,3,
Ivan MILOVIĆ2,3, Slaviša DJURIČIĆ3, Danijela RADIVOJEVIĆ3,
Tanja LALIĆ3, and Milena DJURIĆ2,3
1 Faculty
of Biology, University of Belgrade,
2 Faculty of Medicine, University of Belgrade,
3 Mother and Child Health Institute »Dr Vukan Čupić« , Belgrade,
Serbia and Montenegro
Abstract
Neuroblastoma (NB) is the most frequent childhood solid tumor. The aim of this study was to report on the prognostic significance of clinical parameters (age and stage), genetic [1p deletion and N-myc amplification (NMA] and cytogenetic results in 47 NB patients diagnosed at the Mother and Child Health Institute of Serbia "Dr Vukan Čupić". Clinical factors evaluated in this studies were age and clinical stage. The 5-year overall survival (OS) was best (73%) in the age group children less than 1 year, compared with the older children (15%). Stage IV patients had worst outcome (13%) than »non-stage IV« patients (47%).Genetic factors analyzed in this series of NB patients were: 1p deletion and NMA. 5-year OS was: 65% in the 1p deletion negative group and 13% in the 1p36 deletion positive group; 58% in the NMA negative and 19% in NMA positive group. Cytogenetic results showed that normal karyotype, near-diploidy, near-tetraploidy and homogeneously staining regions (hsr) and double minute chromosomes (dms), together with age over 1 year and stage IV were a very poor prognostic factors.
Key words: neuroblastoma, genetics, cytogenetics
Corresponding author: Marija Guć-Šćekić, Laboratory of Medical Genetics, Mother and Child Health Institute «Dr Vukan Čupić«, Radoja Dakića 6-8, 11070 Belgrade, Serbia and Montenegro, Tel.: 011 3108 272,e-mail: labgenimd@ptt.yu