GENETIKA, Vol. 38, No.1 (2006)
Sanja VASILJEVIĆ, Gordana ŠURLAN-MOMIROVIĆ, Tomislav ŽIVANOVIĆ, Mile IVANOVIĆ, Vojislav MIHAILOVIĆ, Aleksandar MIKIĆ, Slobodan KATIĆ, and Dragan MILIĆ
GENETIC ANALYSIS OF INHERITANCE AND MUTUAL RELATIONSHIPS AMONG YIELD COMPONENTS, MORPHOLOGICAL-BIOLOGICAL TRAITS AND YIELD OF GREEN MASS OF RED CLOVER (TRIFOLIUM PRATENSE L.) [Abstract] [Full text]
S.P. SINGH, Meenakshi SINGH and H.K. YADAV
DIALLEL ANALYSIS FOR SEED YIELD AND ITS COMPONENT TRAITS IN CUPHEA PROCUMBENS [Abstract] [Full text]
R. Ramesh KUMAR and S.P. SINGH
MULTIVARIATE ANALYSIS AND CLUSTERING OF CUPHEA PROCUMBENS INBRED LINES [Abstract] [Full text]
Milica RADOSAVLJEVIĆ
COMPARISON OF AMARANTHUS CRUENTUS AND ZEA MAYS L. STARCH CHARACTERISTICS [Abstract] [Full text]
Slađana ŽILIĆ, Marija MILIVOJEVIĆ, Slađana ŠOBAJIĆ, Milan MAKSIMOVIĆ
EFFECT OF MULTIPLE ALLELES ON OXIDATIVE STABILITY AND GERMINATION OF SOYBEAN SEEDS SUBSEQUENT TO THE ACCELERATED AGEING TEST [Abstract] [Full text]
Dragan NIKOLIĆ
COMPONENTS OF VARIABILITY AND HERITABILITY OF PHENOLOGICAL PHASES IN INTERSPECIES PROGENIES OF F1 GENERATION IN GRAPEVINE [Abstract] [Full text]
Dragana IGNJATOVIĆ-MICIĆ, Ksenija MARKOVIĆ, Vesna LAZIĆ-JANČIĆ
APPLICATION OF MOLECULAR MARKERS IN BULK SEGRAGANT ANALYSIS OF YIELD IN MAIZE (ZEA MAYS L.) SYNTHETIC POPULATIONS [Abstract] [Full text]
Borislav KOBILJSKI, Srbislav DENČIĆ, Jelena PILIPOVIĆ
MOLECULAR SCREENING OF DOMESTIC GERMPLASM FOR ALLELIC VARIANTS AT THE DWARFING GENE Rht8 LOCUS IN WHEAT [Abstract] [Full text]
Zoran KAPOR, Sofija PETROVIĆ, Miodrag DIMITRIJEVIĆ
VARIABILITY OF PLANT HEIGHT AND HARVEST INDEX OF VARIOUS WHEAT GENOTYPES CULTIVATED ON CHERNOZEM AND SOLONETZ [Abstract] [Full text]
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 1-8
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21; 633.63
Original scientific paper
GENETIC ANALYSIS OF INHERITANCE AND MUTUAL RELATIONSHIPS AMONG YIELD COMPONENTS, MORPHOLOGICAL-BIOLOGICAL TRAITS AND YIELD OF GREEN MASS OF RED CLOVER (TRIFOLIUM PRATENSE L.)
Sanja VASILJEVIĆ1, Gordana ŠURLAN-MOMIROVIĆ2,
Tomislav ŽIVANOVIĆ2, Mile IVANOVIĆ1, Vojislav MIHAILOVIĆ1,
Aleksandar MIKIĆ1, Slobodan KATIĆ1, and Dragan MILIĆ1
1 Institute of Field and Vegetable Crops, Novi Sad
2 Faculty of Agriculture, Zemun-Belgrade
Abstract
Sanja Vasiljević, Gordana Šurlan-Momirović, Tomislav Živanović, Mile Ivanović, Vojislav Mihailović, Aleksandar Mikić, Slobodan Katić, and Dragan. Milić (2006): Genetic analysis of inheritance and mutual relationships among yield components, morphological-biological traits and yield of green mass of red clover (Trifolium pratense L.). – Genetika, Vol. 38, No. 1, 1-8.
Diallel crosses involving five divergent red clover genotypes (two varieties, Junior and Diana, and three populations, Vlaška, BL-3 and M-11) were made at the Experiment Field of the Forage Crops Department of the Institute of Field and Vegetable Crops in Novi Sad. During the 2001-2002 period, progenies obtained in the crosses were tested for stem length, stem thickness, length of central lamina, yield of green mass per plant, start of flowering, persistence and growth habit. The analysis of phenotypic variance showed that the additive gene action played the dominant role in the inheritance of stem length, yield of green mass per plant and growth habit. Partial dominance was the most frequent mode of inheritance. A larger contribution of dominant variance to total genotypic variance and low values of narrow sense heritability (hn2) were obtained for stem thickness (16.67% and 23.07%, respectively). In both study years (2001-2002), using path coefficient analysis, we observed significant direct effects of stem length on green mass yield per plant (9.09*, and 6.23*, respectively).
Key words: red clover, (Trifolium pratense L.), diallel cross, mode of inheritance, combining ability, dominant and additive gene effects, heritability, path coefficient analysis
Corresponding author: Sanja VASILJEVIĆ, Institute of Field and Vegetable Crops, Novi Sad
E-mail: sanjava@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 9-22
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22; 633.15
Original scientific paper
DIALLEL ANALYSIS FOR SEED YIELD AND ITS COMPONENT TRAITS IN CUPHEA PROCUMBENS
S.P. SINGH, MEENAKSHI SINGH and H.K. YADAV
Division of Genetics and Plant Breeding,
National Botanical Research Institute (CSIR), Lucknow, India
Abstract
Singh S.P., Meenakshi Singh and H.K. Yadav (2006): Diallel analysis for seed yield and its component traits in Cuphea procumbens. – Genetika, Vol. 38, No.1, 9-22.
The Cuphea procumbens Orteg. is an important annual plant source of medium chain fatty acids. The present study was conducted to estimate different gene systems involved in the inheritance of important quantitative traits viz. plant height, branches/plant, fruits/plant, seeds/fruit and seed yield/plant in F1 and F2 generations following 6 parents half diallel. Diallel assumptions were fulfilled for all the characters. Wr-Vr graph and component analysis revealed the major influence of over dominance for all the traits except branches/plant in F1. The arrays scattered all along the regression line below limiting parabola in two groups. Dominance and recessive and was confirmed by standardized deviation graph. The ranking on the basis of breeding value (Yr) of the parents and per se performance was closely associated (r=0.83**). On the basis of ranking, parents ‘NBC-01’, ‘NBC-25’ and ‘NBC-30’ were found most promising and possessed more dominant alleles for most of the characters. Considering the gene action involved, the breeding plan was discussed
Key words: Cuphea, diallel, regression coefficient, combining ability, gene action, over-dominance
Corresponding author: S.P. Singh, National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, India, Phone: 91-522-2205836:
e-mail: sant_psingh@yahoo.com
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 23-30
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21; 633.63
Original scientific paper
MULTIVARIATE ANALYSIS AND CLUSTERING OF CUPHEA PROCUMBENS INBRED LINES
R. Ramesh KUMAR and S.P. SINGH
Department of Genetics and Plant Breeding,
National Botanical Research Institute (CSIR), Lucknow, India.
Abstract
Kumar S.Ramesh and S. P.Singh (2006): Multivariate analysis and clustering of Cuphea procumbens inbred lines – Genetika, Vol. 38, No. 1, 23-30.
15 C. procumbens inbred lines of different eco-geographical origin maintained at National Botanical Research Institute, Lucknow, India were evaluated for seed yield and its 6 component traits to assess genetic divergence among them. The standardized mean values of different traits were subjected to principal component analysis and cluster analysis was performed based on two different clustering strategies i.e. UPGMA and Wards. Ward’s method that showed relatively high cophenetic correlation coefficient and significant Wilk’s Lambda was identified as the best clustering solution. The first four principal components (PC) with eigenvalues >1 contributed 91.56% of variability among the inbreds. First PC was related with fruits/plant and branches/plant; second PC with yield/ plant, seeds/fruit and test weight; third PC with plant height; and fourth PC with days to flowering. The genotypes were grouped into five clusters and cluster II was largest with 5 genotypes followed by clusters I, III and Clusters IV, V. Cluster IV exhibited highest mean for seed yield (14.77g) followed by cluster III (14.53g) and the former incorporated inbred lines, NBCP-53 and NBCP-58 that were highly divergent among themselves and from genotypes in other clusters. The inbreds in cluster IV and cluster III with good amount of genetic divergence and superior agronomic traits were identified as promising inbreds to develop superior recombinants with desirable agronomic traits.
Key words: Cuphea procumbens, Multivariate analysis, Ward’s Method, Cophenetic correlation coefficient, Wilk’s Lambda
Corresponding author: S.P. Singh, National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, India, Phone: 91-522-2205836:
e-mail: sant_psingh@yahoo.com
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 31-36
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21
Original scientific paper
STARCH ISOLATION AND CHARACTERIZATION COMPARISON BETWEEN AMARANTHUS CRUENTUS AND ZEA MAYS
Abstract
Radosavljević M. (2006): Starch isolation and characterization comparison between amaranthus cruentus and zea mays– Genetika, Vol. 38, No. 1, 31-36.
Starch is a very important, naturally renewable and relatively inexpensive raw material. Since the current industrial production establishes demands pertaining starch quality, a greater attention has been paid to development and improvement of existing technological procedures for starch isolated from different botanical sources.
This paper describes the procedure for amaranth starch isolation. Starch was isolated from Amaranthus cruentus seeds by low alkaline steeping and protease treatments. The properties of isolated amaranth starch were analyzed and compared with those of normal and waxy maize starches.
Key words: Amaranth seeds, starch, alcaline isolation, protease, properties
Corresponding author: Milica Radosavljević, Maize Research Institute “Zemun Polje”, Belgrade – Zemun
e-mail: rmilica@mrizp.co.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 37-48
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21
Original scientific paper
EFFECT OF MULTIPLE ALLELES ON OXIDATIVE STABILITY AND GERMINATION OF SOYBEAN SEEDS SUBSEQUENT TO THE ACCELERATED AGEING TEST
Slađana ŽILIĆ 1, Marija MILIVOJEVIĆ1,
Slađana ŠOBAJIĆ2, Milan MAKSIMOVIĆ3
1Maize Research Institute «Zemun Polje»,
Department of Technology, Zemun Polje, Belgrade-Zemun
2Faculty of Pharmacy, Institute of Bromatology, Belgrade
3Military Medical Academy, Institute of Hygiene
Belgrade, Serbia
Abstract
Žilić Slađana, Marija Milivojević, Slađana Šobajić., Milan Maksimović (2006): Effect of multiple alleles on oxidative stability and germination of soybean seeds subsequent to the accelerated ageing test . – Genetika, Vol. 38, No. 1, 37-48.
The purpose of this study was to determine changes of lipoxygenase activities, contents of antioxidants (tocopherol α, β-carotene and chlorophyll) fatty acids and soluble proteins, as well as, vigour in accelerated aged soybean seeds. The following soybean cultivars were used in the study: Williams 82, Goyou Kurakake and L93-7290.
Subsequent to the accelerated ageing test (AAT), 23% of seeds of the cultivar Goyou Kurakake retained normal germination, while grain of the two remaining cultivars, L93-7290 and Williams 82, completely lost germination ability. According to our results, the seeds of the cultivar Goyou Kurakake (the absence of lipoxygenases 2) is characterised by a significantly higher content of all observed antioxidants (tocopherol α 2.7 mg 100g-1, β-carotene 6.1 μg g-1, chlorophyll 4.9 μg g-1). The contents of β-carotene and chlorophyll decreased after AAT in the seed of the cultivar Goyou Kurakake by 42.8% and 60.5%, respectively, while the content of tocopherol α remained the same. Furthermore, the tocopherol α content was not changed after AAT neither in the cultivar L93-7290 nor in the cultivar Williams 82. The content of β-carotene after AAT remained the same in seeds of the cultivar Williams 82, while it decreased by 7.7% in the cultivar L93-7290. Since changes of the fatty acids content were not pronounced in the cultivar Goyou Kurakake after AAT it can be concluded that the antioxidative system had a crucial role in seeds protection against lipid peroxidation.
Key words: Soybean, lipoxygenase, accelerated ageing, antioxidants
Corresponding author: Slađana Žilić, Maize Research Institute, Zemun Polje, Zemun, Serbia,
Phone: +381-11-3756-704, e-mail: szilic@mrizp.co.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 49-58
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21; 634.23
Original scientific paper
COMPONENTS OF VARIABILITY AND HERITABILITY
OF PHENOLOGICAL PHASES IN INTERSPECIES PROGENIES
OF F1 GENERATION IN GRAPEVINE
Dragan NIKOLIĆ
Faculty of Agriculture, 11080 Belgrade-Zemun, Serbia
Abstract
Nikolić Dragan (2006): Components of variability and heritability of phenological phases in interspecies progenies of F1 generation in grapevine – Genetika, Vol. 38, No. 49 -58.
In four interspecies crossing combinations of grapevine (Seedling 108 x Muscat Hamburg, Muscat Hamburg x Seedling 108, S.V.18315 x Muscat Hamburg and Muscat Hamburg x S.V.12375) during three years period, important phenological phases (bud burst, blooming time, veraison and ripening time) were examined. Based on results of analysis of variance, for all investigated characteristics, components of variability, coefficients of genetic and phenotypic variation and coefficient of heritability in a broader sense were calculated. Values of these parameters depended a lot on examined crossing combination. In majority of all investigated characteristics and almost for all crossing combinations, genetic variability took the biggest part in total variability. Only for blooming time, in crossing combinations Seedling 108 x Muscat Hamburg and Muscat Hamburg x S.V.12375, year variability participated the most in total variability. The lowest coefficients of genetic and phenotypic variation were established for blooming time (4.01%; 4.86%), and the largest for veraison (36.43%; 38.81%). Considering examined crossing combination, coefficient of heritability was from 63.08% up to 70.76% for bud burst, from 60.61% up to 87.71% for blooming time, from 84.58% up to 88.14% for veraison and from 56.86% up to 89.29% for ripening time.
Key words: grapevine, phenological phases, F1 generation, variability, heritability
Corresponding author: Dragan Nikolić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade - Zemun, Serbia
e-mail: nikolicd@agrifaculty.bg.ac.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 59-66
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22; 633.11
Original scientific paper
Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L.) synthetic populations
Dragana IGNJATOVIĆ-MICIĆ, Ksenija MARKOVIĆ, Vesna LAZIĆ-JANČIĆ
Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade, Serbia and Montenegro
Abstract
Ignjatović - Micić Dragana, Marković Ksenija, Lazić - Jančić Vesna (2006): Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L.) synthetic populatins. – Genetika, Vol. 38, No. 59 -66.
Chromosome regions which carry potential QTLs for high grain yield in two synthetic maize populations - B73xMo17 and L1xMo17, were identified by bulk segregant analysis (BSA). Yield was evaluated on F2 testcross families in field trials using a Nested design. Based on yield data, F3 families with the corresponding highest and lowest testcross yields were selected for BSA. Genome analysis of F3 families was carried out with 58 RFLP markers. Allele frequency differences were detected at four RFLP loci n chromosomes 1, 2, 6 and 10 (B73xMo17), i.e. four RFLP loci on chromosomes 1, 2, 6 i 9 (L1xMo17). Only one region, at chromosome 6, was identified in both populations, but with two different RFLP markers. In B73xMo17 it was umc65 and in L1xMo17 umc21 RFLP marker. Bulk segregant analysis was shown to be a quick and informative method for identification of chromosome regions which determine high yield expression in maize, i.e. for identification of RFLP markers closely linked to potential genes involved in expression of the trait.
Key words: maize, grain, yield, BSA, RFLP, chromosome region
Corresponding author: Dragana Ignjatović-Micić,, Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade, Serbia and Montenegro
Phone: 011/3756704,e-mail: idragana@mrizp.co.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 67-74
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22; 633.11
Original scientific paper
1Institute of Field and Vegetable Crops, Novi Sad
2Faculty of Natural Sciences – Novi Sad, Serbia
Abstract
In last 7 years, researches revealed the presence of 16 allelic variants at the Xgwm 261 locus with 165, 174, 180, 192, 194, 196, 197, 198, 200, 201, 202, 204, 205, 207, 210 and 215 base pairs, while nearly 90% of the worldwide wheat germplasm carry one of the alleles with 165bp, 174bp or 192bp. The results of microsatellite screening of 269 wheat varieties and advanced lines from Serbia and Montenegro showed clear selection pressure for the three main fragments at Rht8 locus, but also trend to expand allelic variability in the locus (in the most recent varieties and advanced lines) avoiding «adaptive uniformity» of the elite germplasm. The presence of 192bp allele as diagnostic of Rht8 has been confirmed as quite common in wheat genotypes from Serbia and Montenegro, but not at the rate it has been determined in earlier researches. Out of 269 registered wheat varieties and advanced lines 73.6% carry 192bp allele, 14.9% are with 174bp allele, 7% carry 165 fragment and 4.56% novel fragments around 200bp in length. This research revealed that from the beginning of the 1990s wheat breeding objectives in Serbia and Montenegro were partially focused towards novel alleles (different from 192bp) at Xgwm261 locus. Recently registered varieties from Novi Sad have either the 192bp fragment (Cipovka, Simfonija, Balada, Arija, Rapsodija, Helena, Diva, Vila, Astra), 174bp fragment (Sofija, Dragana, Italija, Francuska, Oda) or fragments around 200bp (Sonata, Kantata, Jefimija). In addition, two very promising advanced lines (NS 260/02 and NS 2-4629/1) and some excellent advanced lines frequently used as parents in hybridization (NS 20/96, NS 23/94, NS 85/97 and L152/89) also carry allele around 200bp in size.
Key words: dwarfing genes, microsatellite, Ppd-D1 gene, Rht8 gene , wheat
Corresponding author: Borislav Kobiljski , Institute of field and vegetable crops, Maksima Gorkog 20; Novi Sad, Serbia
e-mail: kobboris@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 38, No.1 (2006), pp. 75-82
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.21
Original scientific paper
VARIABILITY OF PLANT HEIGHT AND HARVEST INDEX OF VARIOUS WHEAT GENOTYPES CULTIVATED ON CHERNOZEM AND SOLONETZ
Zoran KAPOR, Sofija PETROVIĆ, Miodrag DIMITRIJEVIĆ
Department of Field and Vegetable Crops, Faculty of Agriculture,
University of Novi Sad, Serbia
Abstract
Kapor Zoran, Sofija Petrović, Miodrag Dimitrijević (2006): Variability of plant height and harvest index of various wheat genotypes cultivated on chernozem and solonetz. – Genetika, Vol. 38, No. 1, 75-82.
Solonetz is a type of halomorphic soil which is present on 80 000 ha in Vojvodina. Field experiments were conducted during 1999/2000 vegetation periods, involving eleven wheat genotypes cultivated on solonetz, location Kumane, and on chernozem, location Rimski Sancevi. The variability of plant height and harvest index on different soil types was investigated, as well as the correlation between these two yield parameters. The goal of this research was to establish the significance of various genotypes and soil type effects on plant height and harvest index, as well as the reaction of genotypes regarding different amelioration rates on solonetz.
Key words: solonetz, chernozem, plant height, harvest index, wheat.
Corresponding author: Zoran Kapor, Chair for Genetics and Plant Breeding, Department of Field and Vegetable Crops, Faculty of Agriculture, Trg Dositeja Obradovica 8, University of Novi Sad. Phone number 064/118-6114;
e-mail: zkapor@neobee.net