GENETIKA, Vol. 39, No.1 (2007)
Dejana SAFTIĆ – PANKOVIĆ
APPLICATION OF MOLECULAR MARKERS IN SUNFLOWER BREEDING [Abstract] [Full text]
Rade MILETIĆ, Milisav MITROVIĆ, Milan RAKIĆEVIĆ, Miladin BLAGOJEVIĆ and Žaklina KARAKLAJIĆ-STAJIĆ
THE STUDY OF POPULATIONS OF HAZELNUT C. AVELA L. AND TURKISH HAZELNUT C. COLURNA L. AND THEIR SELECTION [Abstract] [Full text]
Dragana OBREHT, Borislav KOBILJSKI, Mihajla DJAN and Ljiljana VAPA
IDENTIFICATION OF GLU-B1 ALLELES IN BREAD WHEAT CULTIVARS USING PCR [Abstract] [Full text]
Branislav KOVAČEVIĆ, Vojislav GUZINA, Marija KRALJEVIĆ-BALALIĆ and Mile IVANOVIĆ
VARIABILITY AND RELATIONSHIP AMONG CUTTINGS' ROOTING CHARACTERS FOR EASTERN COTTONWOOD [Abstract] [Full text]
Milisav MITROVIĆ, Rade MILETIĆ, Milan RAKIĆEVIć, Miladin BLAGOJEVIĆ, and Ivana GLIŠIĆ
BIOLOGICAL AND POMOLOGICAL PROPERTIES OF SOME WALNUT SELECTIONS FROM THE NATIVE POPULATION [Abstract] [Full text]
Gordan ZEC, Slavica ČOLIĆ, Zoran JANKOVIĆ, and Snežana ĐUROVIĆ
THE EFFECTS OF THE PARENTS’ GENOTYPE ON SUSCEPTIBILITY TO LEAF CURL (TAPHRINA DEFORMANS (BERK.) TUL) OF VINEYARD PEACH HYBRID SEEDLINGS [Abstract] [Full text]
Ana MARJANOVIĆ-JEROMELA, Radovan MARINKOVIĆ and Dragana Miladinović
COMBINING ABILITIES OF RAPESEED (BRASSICA NAPUS L.) VARIETIES [Abstract] [Full text]
Nenad PAVLOVIĆ, Dejan CVIKIĆ, Milan ZDRAVKOVIĆ, Radiša ĐORĐEVIĆ and Slaven PRODANOVIĆ
VARIABILITY AND HERITABILITY COEFFICIENT OF AVERAGE DRY MATTER CONTENT IN ONION (ALLIUM CEPA L.) BULBS [Abstract] [Full text]
Tatjana LAZIĆ and Đurđina RUŽIĆ
ORGANOGENESIS IN VITRO FROM THE LEAF OF BLACKBERRY CV ČAČANSKA BESTRNA [Abstract] [Full text]
Jelena MILOVANOVIĆ, Vasilije ISAJEV, Diana KRAJMEROVÁ, and Ladislav PAULE
ALLELE POLYMORPHISM OF NAD1 GENE OF THE SERBIAN SPRUCE MITOCHONDRIAL GENOME [Abstract] [Full text]
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 1-12
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633
Original scientific paper
Dejana SAFTIĆ-PANKOVIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
The results of the application of molecular markers in sunflower breeding obtained in the Institute of Field and Vegetable Crops in the last decade are reviewed. Our results on genetic distance (GD=7-75%) between sunflower inbred lines obtained with RAPD and SSR markers, indicate large variability and provide important information for the selection of parental lines for future crosses. Interspecific hybridization is often used in sunflower breeding. As only some populations of H. giganteus and H. maximiliani are resistant to sunflower diseases, the investigation of genetic variability in/between two species is of interest. The results obtained with SSR markers are presented. The successful hybridization between H. rigidus and H. annuus was confirmed with RAPD markers, and the variability between F1 and BC1F1 plants is discussed. Desirable alleles and haplotypes can be detected with molecular markers both in early phases of plant development and in early phases of the production of improved lines, which reduces or completely eliminates the large number of testing cycles for desirable phenotypes. CAPS markers for resistance to downy mildew, that can be used in marker assisted selection are presented.
Key words: genetic variability, inbred lines, wild species, RAPDs, SSRs
Corresponding author: Dejana Saftić-Panković, Institute of Field and Vegetable crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 4898416; fax 381 21 413833;
e-mail: dejanapa@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 13-22
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 634.54
Original scientific paper
THE STUDY OF POPULATIONS OF HAZELNUT C. avelana L. AND TURKISH HAZELNUT C. colurna L. AND THEIR SELECTION
Rade MILETIĆ, Milisav MITROVIĆ, Milan RAKIĆEVIĆ, Miladin BLAGOJEVIĆ and Žaklina KARAKLAJIĆ-STAJIĆ
ARI SERBIA. Fruit and Grape Research Centre, Čačak, Srbija
Abstract
Miletić R., M. Mitrović, M. Rakičević, M.Blagojević and Ž. Karaklajić - Stajić (2007): The study of populations of hazelnut C. avelana L. and Turkish hazelnut C. colurna L. and their selection.– Genetika, Vol. 39, No. 1, 13 - 22.
Properties of populations of hazelnut C. avelana L. and Turkish hazelnut C. colurna L. in different regions of Serbia were studied over 1998 – 2005. Round-fruit selections suitable for candy industry were concurrently singled out. Populations of hazelnut and Turkish hazelnut are characterized by small or medium large fruits with round shape index and kernel index ranging from 0.77 – 0.89 and 0.78 – 0.84 respectively.
Singled out selections of hazelnut and Turkish hazelnut have averagely medium large fruits and kernel. Round shape index of fruits and kernel of hazelnut amount to 0.98 (1.01 – 0.91) and 0.99 (1.00 – 0.96) respectively, whereas the same parameter in Turkish hazelnut amounts to 0.99 (0.99 – 0.97) and 0.98 (0.98 – 0.97). Average fruit weight of the singled out selections is 1.79 g (hazelnut) and 1.74 g (Turkish hazelnut). Average kernel mass is 0.75 – 0.70 g. i.e. kernel ratio in hazelnut and Turkish hazelnut amounts to 41% and 40.2% respectively. The mineral matter content in the kernel of the selections amounted to 2.4% and 2.3% respectively. Raw proteins content ranged from 13.8% - 12.4% respectively, and oil content ranged from 47.7 – 50.2% respectively. The majority of indicators under in situ conditions suggest that all selections singled out from the population deserve attention with objective of more intensified propagation and wider introduction into commercial production. It particularly refers to the round shaped kernel which is the most suitable for the candy industry.
Key words: Population, hazelnut, Turkish hazelnut, fruit, round shape index.
Corresponding author: Rade Miletić, ARI Serbia Fruit and grape research Centre, 32000 Čačak, Serbia
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 23-28
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633.11
Original scientific paper
IDENTIFICATION of Glu-B1 alleles IN bread wheat cultivars using PCR
Dragana OBREHT 1,3, Borislav KOBILJSKI 2, Mihajla DJAN 1 and Ljiljana VAPA1
1Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
2Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Obreht D., B. Kobiljski, M. Djan, and Lj. Vapa (2007): Identification of Glu-B1 alleles in bread wheat cultivars using PCR.– Genetika, Vol. 39, No. 1, 23 - 28.
High dough strength is used as a predictor of good quality bread wheat and it has been attributed largely to the type of allele present at the Glu-D1 locus, where the Glu-D1d allele is most favorable. Recently, it has been observed that cultivars with over expressed subunit Bx7OE at the Glu-B1 have enhanced dough strength. In order to implement recently developed PCR assay for Glu-B1 x-type allele discrimination in marker-assisted selection, a set of wheat cultivars from 11 countries was analyzed. The PCR results matched the known Glu-B1 HMW GS genotypes of the cultivars possessing subunits Bx7 or Bx17. It was shown that this molecular marker also differentiated some other Glu-B1 alleles: Bx6, Bx13, Bx20 and Bx22. These results create the opportunity for an improved method of polymorphism scoring at the Glu-B1 in bread wheat germplasm.
Key words: wheat, HMW GS, Glu-B1, marker-assisted selection
Corresponding author: Dragana Obreht, Faculty of Sciences, Department of Biology and Ecology, 21000 Novi Sad, Serbia,e-mail: obrehtd@ib.ns.ac.yu
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 29-38
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633
Original scientific paper
VARIABILITY AND RELATIONSHIP AMONG CUTTINGS' ROOTING CHARACTERS FOR EASTERN COTTONWOOD
Branislav KOVAČEVIĆ1, Vojislav GUZINA1, Marija KRALJEVIĆ-BALALIĆ2 and Mile IVANOVIĆ3
1 Institute for Lowland Forestry and Environment, Faculty of Agriculture, Novi Sad, Serbia
2 Faculty of Agriculture, Novi Sad, Serbia
3 Institute for Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Kovačević B., V. Guzina, M. Kraljević -Balalić, and M. Ivanović (2007): Variability and relationship among cuttings' rooting characters for eastern cottonwood. – Genetika, Vol. 39, No. 1, 29 -38.
The influence of genotype, year and genotype x year interaction on variation of 14 characters of hardwood cutting rooting ability in field conditions, as well as relationship among them was examined in four dates of observation during the first half of vegetation period for fifteen genotypes of Populus deltoides Bartr. are presented. The influence of year rose throughout the examined period, while influence of genotype x year interaction was usually weak. Most of characters had high heritability in second date of observation (second half of May). Beside total number of first-order roots, results signify number of roots on middle and basal part of cutting, as well as shoot characters (number of leaves and shoot height), while characters describing wound roots (roots on basal cut) appeared insignificant. Examined characters were grouped according to PCA (Principal Component Analysis) in two groups, defined by number of roots in the middle part of cutting and number of roots on basal part of cutting.
Key words: Populus deltoides, cutting's rooting, variability, PCA
Corresponding author: Branislav Kovačević, Institute for Lowland Forestry and Environment, Faculty of Agriculture, Novi Sad, Serbia, E-mail: branek@uns.ns.ac.yu, Tel./Fax +381 21 540 383
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 39-46
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 634.5
Original scientific paper
BIOLOGICAL AND POMOLOGICAL PROPERTIES OF SOME WALNUT SELECTIONS FROM THE NATIVE POPULATION
Milisav MITROVIĆ, Rade MILETIĆ, Milan RAKIĆEVIĆ, Miladin BLAGOJEVIĆ, and Ivana GLIŠIĆ
ARI SERBIA, Fruit and Grape Research Centre, Čačak, Serbia
Abstract
Mitrović M., R. Miletić, M. Rakičević, M. Blagojević, and I. Glišić (2007): Biological and pomological properties of some walnut selections from the native population, Vol. 39, No. 1, 39- 46.
The paper presents major biological and pomological properties of walnut selections singled out from the region of Central and Eastern Serbia over 2002 – 2005. The selections are abundant croppers of extremely high vigour. The vegetation cycle begins mainly in the third decade of April, whereas flowering and inflorescence formation occur in first decade of May. Fruits ripen in the third decade of September. These selections are extremely resistant or resistant to low winter temperatures and resistant to moderately resistant to Gnomonia leptostyla. The fruits are mainly medium large or large with fruit weight ranging from 14.0 – 11.3 g. The kernel is light coloured or yellow of good or very good taste, with high kernel ratio (50.0 – 45.1%). The majority of these selections have high oil content (68.2 – 61.8%) and medium raw protein content (18.3 – 15.4%). All the stated selections should be taken into consideration in respect of collection establishment and deserve to be excessively introduced into production upon testing under the respective conditions.
Key words: Walnut, population, selection, fruit, kernel
Corresponding author: Milisav Mitrović, ARI SERBIA, Fruit and Grape Research Centre,32000 Čačak, Serbia
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 47-52
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 634.25
Original scientific paper
THE EFFECTS OF THE PARENTS’ GENOTYPE ON SUSCEPTIBILITY TO LEAF CURL (Taphrina deformans (BERK.) TUL) OF VINEYARD PEACH HYBRID SEEDLINGS
Gordan ZEC, Slavica ČOLIĆ, Zoran JANKOVIĆ, and Snežana ĐUROVIĆ
Institut PKB Agroekonomik, Padinska Skela, Serbia
Abstract
Zec G., S. Čolić, Z. Janković, and S. Đurović (2007): The effects of the parents’ genotype on susceptibility to leaf curl (Taphrina deformans (Berk.) tul) of vineyard peach hybrid seedlings. – Genetika, Vol. 39, No. 1, 47 - 52.
Leaf curl is one of the peach diseases with the most economically important impact, because very strong infection can cause total defoliation. Susceptibility to a leaf curl was examined from the period 1994-1998 in a collection of selected vineyard peach genotypes and newly peach and nectarine cultivars. Selected low susceptible genotypes (vineyard peach GR/65/87 and processing peach Villa Ada) were used in cross-pollination with differently susceptible genotypes. The process produced more than 250 seedlings of F1 progeny from few combinations of the parents. Susceptibility of hybrid seedlings to the leaf curl pathogen was investigated several years in the open field, without application of pesticide. More types of inheritance of examined characteristic were determinate in the progenies from different combinations of the parents. The results agree with polygenic inheritance.
Key word: peach, leaf curl, inheritance
Corresponding author: Gordan Zec, Institut PKB Agroekonomik, Industrijsko naselje b.b., 11213 Padinska Skela, Srbija tel: 011 8871 175, e-mail: zec2004@yahoo.com
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 53-62
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
Combining abilities of rapeseed (Brassica napus L.) varieties
Ana Marjanović - Jeromela, Radovan Marinković, and Dragana Miladinović
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Marijanovic-Jeromela A., R. Marinkovic, and D. Miladinovic (2007): Combining abilities of rapeseed (Brassica napus L.) varieties. – Genetika, Vol. 39, No. 1, 53 -62.
The global acreage under rapeseed increases steadily. Rapeseed is grown for oil for human consumption, feed and biodiesel production. For faster advances in breeding, it is necessary to know variability and combining ability of selection material i.e. modes of inheritance of certain traits.
General (GCA) and specific combining abilities (SCA) of five rapeseed varieties as well as the mode of inheritance of plant height, height to the first lateral branch, number of lateral branches and seed yield per plant were analyzed in this paper. Positive heterosis for plant height was found in five cross combinations, for the height to the first lateral branch in two combinations and for the number of lateral branches in only one combination and for seed yield in three cross combinations.
Key words: dialel, combining ability, morphometric characteristics, oilseed
Corresponding author: Ana Marijanović-Jeromela, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21 000 Novi Sad, Serbia
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 63-68
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635.25
Original scientific paper
VARIABILITY AND HERITABILITY COEFFICIENT OF AVERAGE DRY MATTER CONTENT IN ONION (Allium cepa L.) BULBS
Nenad PAVLOVIĆ1, Dejan CVIKIĆ1, Milan ZDRAVKOVIĆ1, Radiša ĐORĐEVIĆ1 and Slaven PRODANOVIĆ2
1Agricultural Research Institute "Serbia", Centre for Vegetable Crops,
Smederevska Palanka, 2Faculty of Agriculture, Beograd, Zemun
Abstract
Pavlovic N., D. Cvikic, M. Zdravkovic, R. Đorđevic and S. Prodanovic (2007): Variability and heritability coefficient of average dry matter content in onion (Allium cepa L.) bulbs. – Genetika, Vol. 39, No. 1, 63 - 68.
In order to research the variability of average dry matter content in onion bulbs, the trial has been set up on the experimental plot in the Centre for Vegetable Crops in Smederevska Palanka during 2000. and 2001. The trial has been performed by applying the method of random block design with five replicas. Ten cultivars of different geographical origin have been used. Examined characteristics were determined by applying the variance analysis of two-factorial trial – model 2. Hadzivukovic, 1991. The components of phenotype variance, genotype and phenotype coefficient of variance and heritability in broader sense have been calculated according to Singh and Chaudhary (1976).
The significant variability has been found for dry matter content in bulbs during both years of research. The researched trait had a greater genotype variance than out door conditions factor variance and the coefficient of phenotype variation greater than genetic variance coefficient.
High percentage of genetic variability in total phenotype variability has been confirmed by high heritability.
Key words: onion, dry matter content, coefficient of variation, heritability
Corresponding author: Nenad Pavlović, Agricultural Research Institute "Serbia", Centre for Vegetable Crops, 71 Karadjordjeva St. 11420 Smederevska Palanka
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 69-78
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.71
Original scientific paper
ORGANOGENESIS IN VITRO FROM THE LEAF OF BLACKBERRY CV ČAČANSKA BESTRNA
Tatjana LAZIĆ and Đurđina RUŽIĆ
Fruit Research Institute, Čačak, Serbia
Abstract
Lazić T. and Đ. Ružić (2007): Organogenesis in vitro from the leaf of blackberry cv Čačanska bestrna. – Genetika, Vol. 39, No. 1, 69-78.
The paper presents evaluation of different types and concentrations of plant growth regulators on induction of adventitious organogenesis from the leaf of blackberry cultivar Čačanska Bestrna. This genotype was introduced and maintained in tissue culture on Murashige and Skoog medium (MS) (1962), mineral salts and organic complex with in mg l-1: BAP 1.0, IBA 0.1, GA3 0.1. Young, expanded leaves taken from upper third of in vitro proliferating shoots were used as initial explants. The leaves were cut three times across the midrib and placed with adaxial side touching the regeneration medium. In this experiment 20 types of regeneration media have been evaluated with different types and concentrations of cytokinins and auxins: BAP (2.0 mg l-1) or TDZ (1.0 mg l-1) alone, or each in combination with different concentrations of IBA, NAA and 2,4-D (0.1, 1.0 and 2.0 mg l-1).
In cv Čačanska Bestrna regeneration of adventitious shoots was observed on three different types of media with BAP combined with IBA (0.1 and 1.0 mg l-1) and NAA (0.1 mg l-1). TDZ alone or in combination with IBA (0.1, 1.0 and 2.0 mg l-1) and NAA (0.1 mg l-1) has been more effective at induction of regeneration than BAP. The highest percentage of regeneration was obtained precisely on medium supplemented with TDZ alone (41.66%).
Key words: Cv Čačanska Bestrna, adventitious organogenesis, leaf explants, cytokinins, auxins
Corresponding author: Tatjana Lazić, MSc, Researcher Assoicate, Fruit Research Institute, Kralja Petra I 9, 32000 Čačak, tel: 032/221-375; 227-550; fax: 032/221-391, E-mail: jugvocca@yu1.net: tanjalazic2000@yahoo.com
Published in „GENETIKA“ Vol. 39, No.1 (2007), pp. 79-91
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.2: 633
Original scientific paper
ALLELE POLYMORPHISM OF NAD1 GENE OF THE SERBIAN SPRUCE MITOCHONDRIAL GENOME
Jelena MILOVANOVIĆ, Vasilije ISAJEV, Diana KRAJMEROVÁ1 and Ladislav PAULE1
1Faculty of Forestry, Technical University of Zvolen, Slovakia
Abstract
Milovanović J., V. Isajev, D. Krajmerova, and L. Paule (2007): Allele polymorphism of Nad1 gene of the Serbian spruce mitochondrial genome. – Genetika, Vol. 39, No. 1, 79-91.
Serbian spruce (Picea omorika /Panč./Purkyne), as the Balkan Peninsula endemic and the Tertiary relic, is a species whose survival is threatened by the constant restriction of its range caused by the global changes of environmental conditions and the adverse human impacts. The Serbian spruce seedling seed orchard at Godovik represents the base for the improvement of the production of the selected seeds of this species, which can be used as the initial material for the extension of its range. The allele polymorphism of the mitochondrial nad1 gene was analysed in five different Serbian spruce phenogroups of which the orchard is established. The obtained results are a contribution to a closer study of the causes of the postglacial intraspecific differentiation of Serbian spruce and the creation of the above phenogroups. The study results are significant for further breeding of this species based on the better knowledge of the genetic structure of the species, its directed utilisation and the widening of its range.
Key words: Serbian spruce, mtDNA, intraspecific differentiation