GENETIKA, Vol. 39, No.3 (2007)
Slavica ČOLIĆ and Gordan ZEC
Morphological and pomological traits variability of almond genotypes from Slankamen hill population [Abstract] [Full text]
Milica FOTIRIĆ, Dragan NIKOLIĆ and Vera RAKONJAC
VARIABILITY COMPONENTS AND HERITABILITY OF POMOLOGICAL AND CHEMICAL CHARACTERISTICS IN SOUR CHERRY CLONES OF CULTIVAR MONTMORENCY [Abstract] [Full text]
Evica MRATINIĆ and Milica FOTIRIĆ
SELECTION OF BLACK ELDERBERRY (Sambucus nigra L.) AND EVALUATION OF ITS FRUITS USABILITY AS BIOLOGICALLY VALUABLE FOOD [Abstract] [Full text]
Evica MRATINIĆ, Vera RAKONJAC and Dragan MILATOVIĆ
GENETIC PARAMETERS OF YIELD AND MORPHOLOGICAL FRUIT AND STONE PROPERTIES IN APRICOT [Abstract] [Full text]
Sonja IVANOVSKA, Cane STOJKOVSKI, Zoran DIMOV, Ana MARJANOVIC- JEROMELA, Mirjana JANKULOVSKA, and Ljupco JANKULOSKI
INTERRELATIONSHIP BETWEEN YIELD AND YIELD RELATED TRAITS OF SPRING CANOLA (BRASSICA NAPUS L.) GENOTYPES [Abstract] [Full text]
Dragan NIKOLIĆ and Vera RAKONJAC
DIVERGENCE OF MYROBALAN (Prunus cerasifera Ehrh.) TYPES ON THE TERRITORY OF SERBIA [Abstract] [Full text]
Víctor M. SALCEDA, Judith GUZMÁN and Olga OLVERA.
INVERSION POLYMORPHISM IN SOME NATURAL POPULATIONS OF DROSOPHILA PSEUDOOBSCURA FROM CENTRAL MEXICO [Abstract] [Full text]
Jelena Srdić, Snežana Mladenović Drinić, Zorica Pajić and Milomir Filipović
CHARACTERISATION OF MAIZE INBRED LINES BASED ON MOLECULAR MARKERS, HETEROSIS AND PEDIGREE DATA [Abstract] [Full text]
Veselinka ZEČEVIĆ, Desimir KNEŽEVIĆ, and Danica MIĆANOVIĆ
VARIABILITY OF TECHNOLOGICAL QUALITY COMPONENTS IN WINTER WHEAT [Abstract] [Full text]
Nikola HRISTOV, Novica MLADENOV and Ankica KONDIC-SPIKA
BREEDING ASPECTS OF LOW TEMPERATURE TOLERANCE IN WHEAT [Abstract] [Full text]
Slađana MARIĆ, Radovan BOŠKOVIĆ and Milan LUKIĆ
THE POLYMORPHISM OF ETR1 GENE IN AUTOCHTHONOUS APPLE CULTIVARS [Abstract] [Full text]
Gorica Cvijanović, Nada Milošević and Mirjana Jarak
THE IMPORTANCE OF DIAZOTROPHS AS BIOFERTILISERS IN THE MAIZE AND SOYBEAN PRODUCTION [Abstract] [Full text]
Ankica KONDIĆ- ŠIPKA, Boris KOBILJSKI and Nikola HRISTOV
HIGH PRODUCTION OF WHEAT DOUBLE HAPLOIDS VIA ANTHER CULTURE [Abstract] [Full text]
Dejan DODIG, Miroslav ZORIĆ, Desimir KNEŽEVIĆ, Bojana DIMITRIJEVIĆ and Gordana ŠURLAN–MOMIROVIĆ
ASSESSING WHEAT PERFORMANCE USING ENVIRONMENTAL INFORMATION [Abstract] [Full text]
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 291-296
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 634.55
Original scientific paper
Morphological and pomological traits variability of almond genotypes from Slankamen hill population
Slavica ČOLIĆ and Gordan ZEC
Institute PKB AGROEKONOMIK, Padinska Skela, Serbia
Abstract
Čolić S. and G. Zec (2007): Morphological and pomological traits variability of almond genotypes from Slankamen hill population.– Genetika, Vol. 39, No. 3, 291-296.
Almond production in Serbia is much lower then needs and possibilities and its growing are limited on garden plots. Short period of biological rest is characteristic of almond tree. Because of that its growth can be successful only in some locations in Serbia. One of such places is Slankamen hill, where almost every garden plot has almond seedlings. Spontaneous almond population from Slankamen hill has great variability of morphological and pomological traits. After several years of visiting this location and evaluating of more than 300 almond seedlings, 20 genotypes with good and regular cropping have been selected. During the 2003-06, their morphological and pomological traits were examined. Description was given on the basis of almond descriptor (GÜLCAN, 1985). The research, selection and collection of almond genotypes from this locality should contribute expansion of almond tree growing area in Serbia. Genotypes 1 and 19 with medium fruit and regular crop, as well as the late-blooming genotypes 29 and 13 were selected.
Key words: almond, morphology, pomology, traits, variability
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 297–304
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 634.23
Original scientific paper
VARIABILITY COMPONENTS AND HERITABILITY OF POMOLOGICAL AND CHEMICAL CHARACTERISTICS IN SOUR CHERRY CLONES OF CULTIVAR MONTMORENCY
Milica FOTIRIĆ, Dragan NIKOLIĆ and Vera RAKONJAC
Faculty of Agriculture, Belgrade-Zemun, Serbia
Abstract
Fotrić M., D. Nikolić, and V. Rakonjac (2007): Variability components and heritability of pomological and chemical characteristics in sour cherry clones of cultivar montmorency. – Genetika, Vol. 39, No. 3, 297 - 304.
In 12 sour cherry clones of cultivar Montmorency 5 pomological (fruit length, fruit width, fruit weight, stone weight and fruit stem length) and 4 chemical characteristics (soluble solid content, total acid content, invert sugar content and total sugar content) were investigated. Based on results of analysis of variance for all examined characteristics, variability components, coefficients of genetic and phenotypic variation and coefficient of heritability in a broader sense were calculated. Considering components of total variability, year represented the component that caused variability of majority examined characteristics in the highest percentage. Coefficients of genetic variation showed that in investigated clones total acid content varied the least (CVg = 1.79%), and the most fruit weight (CVg = 11.41%). The lowest phenotypic varying was determined for fruit length (CVf = 4.01%) but the highest for fruit weight (CVf = 13.86%). Coefficient of heritability was the highest for stone weight (h2 = 70.27%), and the lowest for total acid content (h2 = 7.73%).
Key words: clonal selection, heritability, Montmorency, sour cherry, variability
Published in „GENETIKA“ Vol. 39 No.3 (2007), pp. 305-314
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.747
Original scientific paper
SELECTION OF BLACK ELDERBERRY (Sambucus nigra L.) AND EVALUATION OF ITS FRUITS USABILITY AS BIOLOGICALLY VALUABLE FOOD
Evica MRATINIĆ and Milica FOTIRIĆ
Faculty of Agriculture, Belgrade, Serbia
Abstract
Mratinić E. and M. Fotirić (2007): Selection of black elderberry (Sambucus nigra L.) and evaluation of its fruits usability as biologically valuable food . – Genetika, Vol. 39, No. 3, 305-314.
To carry out domestication, five genotypes (B1, B2, R, VG and RK) were isolated from natural populations by selection of black elderberry (Sambucus nigra L.). The most prominent biological-technological characteristics were studied during fruit flowering and ripening. Flowering proceeded in the period from mid-April to the 1st decade of June, while ripening lasted from the 1st decade of August to the 1st decade of October. The largest inflorescences were found in genotype R (14.32 cm in diameter). The highest number of berries per inflorescence (on average, 280), the largest berry (on average, 0.21 g), and the best fruit quality (17.19% dry matter, 11.50% sugars, 35 mg vitamin C, and 2.58 g/l total colored matter) were all demonstrated by genotype VG. In all isolated genotypes fruit demonstrated biologically high quality, which makes it a good raw material desirable for diverse forms of processing. Its high yield level, pronounced resistance to disease agents and pests, as well as adaptability to adverse conditions are a recommendation for organic-based production.
Key words: black elderberry, berry, flowering period, inflorescence, fruit quality selection, ripening
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 315-324
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 634.21
Original scientific paper
GENETIC PARAMETERS OF YIELD AND MORPHOLOGICAL FRUIT AND STONE PROPERTIES IN APRICOT
Evica MRATINIĆ, Vera RAKONJAC and Dragan MILATOVIĆ
Faculty of Agriculture, Belgrade - Zemun, Serbia
Abstract
Mratinić E., V. Rakonjac, and D. Milatović (2007): Genetic parameters of yield and morphological fruit and stone properties in apricot.– Genetika, Vol. 39, No. 3, 315 -324.
Yield, fruit and stone weight and dimensions were studied in 24 apricot cultivars over a four-year period, to determine components of variability, heritability and genetic and phenotypic correlations. The analysis of the components of total variance evidenced that genetic differences between cultivars determined to the highest percentage the variability of fruit height and stone weight and dimensions, whereas the variation of yield, and fruit weight, breadth and thickness was predominantly determined by ecological factors. The values of heritability coefficients, in a broader sense, were found to be relatively high for fruit and stone weight and dimensions (88-93%), and medium for yield (48%). It was established by correlation analysis that all genetic and phenotypic coefficients of correlation between fruit and stone weight and dimensions were positive and statistically very significant. Yield was in a very significant, positive genetic correlation with a majority of studied characters except for fruit breadth. However, the phenotypic coefficients of correlation between those properties were not statistically significant.
Key words: components of variability, coefficients of correlation, heritability, Prunus armeniaca L.
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 325-332
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633
Original scientific paper
INTERRELATIONSHIP BETWEEN YIELD AND YIELD RELATED TRAITS OF SPRING CANOLA (BRASSICA NAPUS L.) GENOTYPES
Sonja IVANOVSKA1, Cane STOJKOVSKI1, Zoran DIMOV1, Ana MARJANOVIC- JEROMELA2, Mirjana JANKULOVSKA1, and Ljupco JANKULOSKI1
1 Faculty for Agricultural Sciences and Food, Skopje, Macedonia
2 Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Ivanovska S., C. Stojkovski, Z. Dimov, A. Marijanović-Jeromela, M. Jankulovska and Lj. Jankuloski (2007): Interrelationship between yield and yield related traits of spring canola (Brassica napus L.) genotypes.– Genetika, Vol. 39, No. 3, 325 -332.
The research was conducted in order to determine yield related characters which are more effective as selection criteria than yield itself. Three spring canola (Brassica napus L.) genotypes have been investigated for plant height, number of primary branches (including the main raceme), number of pods per plant, pod length, number of seeds per pod, seed weight per pod and 1000 seed weight and their correlations with seed yield per plant. Seed yield had highest correlation with number of pods per plant (r= 0.935 and r= 0.973 in Skopje and Strumica, respectively), followed by seed weight per pod (r= 0.693 and r= 0.729) and 1000 seed weight (r= 0.627 and r= 0.680). Path coefficient analysis was used to identify the direct and indirect effect of studied characters on seed yield. Number of pods per plant (p=0.759 and p=0.877 for Skopje and Strumica, respectively) and seed weight per pod (p=0.216 and p=0.225) had the highest direct effect on seed yield in both locations followed by 1000 seed weight (p=0.056 and p=0.010). The coefficient of determination (R2) was 0.943.
Key word: path-coefficient, spring oilseed rape, simple correlation coefficient
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 51-58
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 634.22
Original scientific paper
DIVERGENCE OF MYROBALAN (Prunus cerasifera Ehrh.) TYPES ON THE TERRITORY OF SERBIA
Dragan NIKOLIĆ and Vera RAKONJAC
Faculty of Agriculture, Belgrade-Zemun, Serbia
Abstract
Nikolić D. and V. Rakonjac (2007): Divergence of myrobalan (Prunus cerasifera ehrh.) types on the territory of Serbia.. – Genetika, Vol. 39, No. 3, 333 - 342.
Variability of some more prominent pomological characteristics was examined in three regions of Serbia (central, western, southern). In all the regions, significant variability of all studied characteristics was established. However, no specifics were manifested between regions, therefore, identical types emerge in all the regions. This is indicated by similar intervals of variation as well as similar mean values of characteristics per region. The obtained results lead to the conclusion that the entire territory of Serbia should be observed as a unique myrobalan population with highly expressed polymorphism of characteristics. To preserve genetic variability of myrobalan, collection is recommended for those types that were arranged into various groups and subgroups according to the results of cluster analysis.
Key word: myrobalan, natural population, pomological characteristics, phenotypic variability
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 343-354
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 596.773
Original scientific paper
INVERSION POLYMORPHISM IN SOME NATURAL POPULATIONS OF DROSOPHILA PSEUDOOBSCURA FROM CENTRAL MEXICO
Víctor M. SALCEDA1, Judith GUZMÁN2 and Olga OLVERA1.
1Departamento de Biología, Instituto Nacional de Investigaciones Nucleares, México
2Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México
Abstract
Salceda V., J. Guzman and O. Olivera (2007): Inversion polymorphism in some natural populations of Drosophila pseudoobscura from central Mexico. – Genetika, Vol. 39, No. 3, 343 -354.
Samples of D. pseudoobscura were taken in seventeen localities in Central Mexico inside the parallels 18o – 20o N, with the purpose of determine the chromosomal polymorphism in the third of the different populations of this species. From each captured female a single larva of its offspring was taken, its salivary glands extracted and stained with a solution of aceto orcein to observe the polytene chromosomes. From these smears the corresponding karyotype of each larva was determined, keeping a record of them. With the information gathered the relative frequency of each one of the fourteen different inversions found was calculated. A grand total of 1894 third chromosomes were analyzed. The fourteen different inversions found are equivalent to a 34.1 % of the total chromosomal variation of the species. The most abundant inversions found were: TL 50.6 %, CU 27.2 5, SC 9.1 % and EP 5.5 %; the remaining ten inversions detected are in general grounds rare ones with variable relative frequencies depending on the locality. Analysis of the predominant inversions for each population was done. The presence of West-East gradients is reported, even if in cases not so well defined, since as one moves in a particular direction the ups and downs in relative frequency for the alternating pairs TL-CU; TL-SC in the western populations and TL-CU in the eastern ones were observed. The assignment of each population to a particular race was also done, and such a way we were able to recognize three different races coexisting in the area of study.
Key word: Drosophila pseudoobscura, natural populations, polymorphism
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 355-363
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633.15
Original scientific paper
CHARACTERISATION OF MAIZE INBRED LINES BASED ON MOLECULAR MARKERS, HETEROSIS AND PEDIGREE DATA
Jelena Srdić, Snežana Mladenović Drinić, Zorica Pajić and Milomir Filipović
Maize Research Institute „Zemun Polje“ Belgrade, Serbia
Abstract
Srdić J., S. Mladenović Drinić, Z. Pajić, and M. Filipović (2007): Characterization of maize inbred lines based on molecular markers, heterosis and pedigree data. – Genetika, Vol. 39, No. 3, 355 - 363.
Information about the genetic diversity of inbred lines is essential in planning maize breeding programmes. Utilization of diverse parents in the process of hybridization has the greatest influence on producing high yielding hybrids.
The aim of this research was to determine genetic diversity of ten maize inbred lines of different origin on the basis of protein and RAPD markers and to compare these results with pedigree and grain yield heterosis data. Results of genetic distances, based on protein and RAPD markers were similar and in concurrence with the date on the origin of inbreds.
Usefulness of protein and RAPD markers for assigning inbreds to heterotic groups was examined by the cluster analysis. Cluster analysis based on protein markers, RAPD and heterosis showed clear grouping of lines into two main heterotic groups. Only few deviations were noticed, and those among inbreds not belonging to those heterotic groups. According to the observed results it could be concluded that grouping of inbred lines based on molecular markers, generally agrees with their pedigrees and that clusters are representatives of hetrotic groups. Very high and highly significant estimate of rank correlation coefficient between RAPD and heterosis (0,876**) also confirmed that.
Key word: cluster analysis, heterotic groups, molecular markers, RAPD
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 365-374
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633.11
Original scientific paper
VARIABILITY OF TECHNOLOGICAL QUALITY COMPONENTS IN WINTER WHEAT
Veselinka ZEČEVIĆ1, Desimir KNEŽEVIĆ2, and Danica MIĆANOVIĆ3
1Small Grains Research Centre, Kragujevac
2 Faculty of Agriculture, University of Priština, Zubin Potok
3 Serbian Chamber of Commerce, Belgrade, Serbia
Abstract
Zečević V., D. Knežević and D. Mičanović (2007): Variability of technological quality components in winter wheat. – Genetika, Vol. 39, No. 3, 365 - 374.
Ten winter wheat cultivars created in Small Grains Research Centre of Kragujevac (KG-56, Srbijanka, Studenica, Takovčanka, KG-56S, KG-100, Toplica, Levčanka, Gruža, and Tara) were grown at experimental field during four years. Variability of bread-making quality properties (sedimentation value, gluten content and quality, and rheological flour and dough properties) was investigated. The sedimentation value was determined by Zeleny method, gluten content by standard method, and rheological flour and dough properties by Farinograph. Quality components depended significantly of genotype and environment factors. Obtained results have shown that the highest value of sedimentation in average was at KG-56S cultivar (61.8ml). Sedimentation in all investigated cultivars was at the level of the first quality class. Wet gluten are characterized by good physical properties, and on average ranged from 25.6% (KG-100) to 36.3% (Gruža). Wheat technological quality depended predominantly of genetic potential of cultivar, and it was on the level of A2-B2 quality group. The highest impact of phenotypic variance belonged to genotype for sedimentation value, wet gluten content and rheological flour and dough properties, while for water absorption belonged to genotype-year interaction.
Key word: gluten, quality, rheological properties, sedimentation, variability, wheat
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 375-386
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633.11
Original scientific paper
BREEDING ASPECTS OF LOW TEMPERATURE TOLERANCE IN WHEAT
Nikola HRISTOV, Novica MLADENOV and Ankica KONDIC-SPIKA
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Hristov N., N. Mladenov, and A. Kondić-Šipka (2007): Breeding aspects of low temperature tolerance in wheat. – Genetika, Vol. 39, No. 3, 375 - 386.
Wheat resistance to low temperatures is complex in nature and dependant on variety as well as on interaction with the environment. In unfavorable conditions for hardening, even varieties resistant to cold may exhibit higher susceptibility to low temperatures. Over a two-year period (2003-04), tests were conducted in cold chamber at–15˚C lasting 24, 36, 48 and 60 hours. The 23 wheat varieties analyzed were divided into four groups according to year of release. In the first group, which was mostly made up of foreign introductions, the average plant survival rate across treatments was 87%. The different origins of the varieties from this group were a significant factor behind the differences in their resistance levels. The second group, comprised of the first domestic varieties, had an average plant survival rate of 89%, while the third, containing the dominant varieties from the 1980s, had a plant survival rate of 88.4% on average. In the fourth group, consisting mostly of varieties that are currently commercially grown in the country, the average plant survival rate was 86.6%. Thanks to high temperatures present during the process of hardening, there was greater plant damage and percent winterkill in the second year than in the first. The resistance of the varieties to low temperatures has been maintained at a level enabling successful and stable production under the cold temperature stress conditions of southeastern Europe.
Key word: breeding, low temperatures, variety, wheat
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 387-394
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 634.11
Original scientific paper
THE POLYMORPHISM OF ETR1 GENE IN AUTOCHTHONOUS
APPLE CULTIVARS
Slađana MARIĆ1, Radovan BOŠKOVIĆ2 and Milan LUKIĆ1
1Fruit Research Institute, Čačak
2Institute of Molecular Biology and Genetic Engineering, Belgrade, Serbia
Abstract
Marić S., R. Bošković, and M. Lukić (2007): The polymorphism of ETR1 gene in autochthonous apple cultivars. – Genetika, Vol. 39, No. 3, 387 - 394.
Ethylene is a plant hormone, which plays an important role in the ripening of climacteric fruits such as the apple. We studied allelic polymorphism of the ETR1 gene, encoding ethylene receptor, in 23 autochthonous apple cultivars. The polymorphism was revealed by combining the gene specific PCR and restriction of PCR product. Four alleles of the ETR1 gene (a, b, c and d) were detected, and their possible association with the fruit storage ability examined.
Key word: apple, ethylene, ETR1 gene, polymorphism, fruit ripening.
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 395-404
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633
Original scientific paper
THE IMPORTANCE OF DIAZOTROPHS AS BIOFERTILISERS IN THE MAIZE AND SOYBEAN PRODUCTION
Gorica Cvijanović1, Nada Milošević2 and Mirjana Jarak3
1Maize Research Institute, Belgrade-Zemun
2institute of Field and Vegetable Crops, Novi Sad
3faculty of Agriculture, University of Novi Sad
Abstract
Cvijanović G., N. Milošević and M. Jarak (2007): The importance of diazotrophs as biofertilisers in the maize and soybean production. - Genetika, Vol 39, No. 3, 395-404.
The contemporary food production requires the preservation of soil productivity with the simultaneous maintenance of the yield level accomplished with the appropriate fertilising. The maize and soybean production is unimaginable without fertilisers and the application of information within the filed of nitrogen fixation. The application of fertilisers has been increasing. Diazotrophs are microorganisms with the ability to fix atmospheric nitrogen and to convert it in forms available to plants. Therefore, effects of different rates of mineral nitrogen (80, 120 and 160 kg N ha-1 in maize and half of the mentioned rates in soybean), as well as, maize seed bacterisation with the associative species (Azotobacter chroococcum, Azospirillum lipoferum, Klebsiella planticola, Beijerinckia derxi) and soybean with the symbiotic species (Bradyrhizobium japonicum) and their mixture on soil biogeny and yield quality and quantity were studied.
The studied parameters in maize had higher values under conditions of bacterisation and fertilisation with 80 kg N ha-1, while the mixture of diazotrophs and fertilisation with 40 kg N ha-1 resulted in higher values of studied parameters in soybean. It is possible to produce organic/healthy food with the maintenance of soil biogeny if diazotrophs are incorporated into the soil with lower rates of mineral nitrogen. This possibility is a basic prerequisite for sustainable agriculture.
Key word: bacterisation, biogeny, maize, soybean, yield
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 405-411
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.22: 633.11
Original scientific paper
HIGH PRODUCTION OF WHEAT DOUBLE HAPLOIDS VIA ANTHER CULTURE
Ankica KONDIĆ- ŠIPKA, Boris KOBILJSKI and Nikola HRISTOV
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Kondić-Šipka A., B. Kobiljski and N. Hristov (2007): High production of wheat double haploids via anther cultur. – Genetika, Vol. 39, No. 3, 405 - 411.
Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium.
The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively).
Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30-Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana).
As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breeding programs for rapid production of homozygous wheat lines.
Key word: anther culture, doubled haploid, wheat
Published in „GENETIKA“ Vol. 39, No.3 (2007), pp. 413-425
© 2007 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633.11
Original scientific paper
ASSESSING WHEAT PERFORMANCE USING ENVIRONMENTAL INFORMATION
Dejan DODIG1, Miroslav ZORIĆ2, Desimir KNEŽEVIĆ3, Bojana DIMITRIJEVIĆ2 and Gordana ŠURLAN–MOMIROVIĆ2
1Maize Research Institute Zemun Polje, Belgrade
2 Institute of Field Crop Science, Faculty of Agriculture, Belgrade
3 Faculty of Agriculture, University of Priština, Zubin Potok
Abstract
Dodig D., M. Zorić, D. Knežević, B. Dimitrijević and G. Šurlan Momirović (2007): Assessing wheat performance using environmental information. – Genetika, Vol. 39, No. 3, 413-425.
The partial least squares (PLS) regression model was applied to wheat data set with objective to determining the most relevant environmental variables that explained biomass per plant and grain yield genotype × environment interaction (GEI) effects. The data set had 25 wheat genotypes (20 landraces + 5 cultivars) tested for 4 years in two different water regimes: rained and drought. Environmental variables such as maximum soil temperature at 5 cm in April and May, soil moisture in the top 75 cm in March, and sun hours per day in May accounted for a sizeable proportion of GEI for biomass per plant. Similar results were obtained for grain yield: maximum soil temperature at 5 cm in April, May and June, and sun hours per day in May were related to the factor that explained the largest portion (>38%) of the GEI. Generally, wheat landraces are able to better exploit environments with higher temperatures and lower water availability during vegetative growth (March-June) than cultivars.
Key word: biomass, GEI, grain yield, PLS regression, wheat