N. TUCIĆ
POPULATION GENETIC VARIABILITY IN COLD TEMPERATURE RESISTANCE AT DIFFERENT DEVELOPMENTAL STAGES OF DROSCPHILA MELANOGASTER [Abstract]
V. KEKIĆ and D. MARINKOVIĆ
SELECTION FOR »NEGATIVE« PHOTOTAXIS IN DROSOPHILA SUBOBSCURA [Abstract]
Katarina BOROJEVIĆ
GENETIC STRUCTURE OF POPULATIONS OF TRITICUM AESTIVUM ssp. VULGARE AFTER MUTAGENIC TREATMENTS [Abstract]
D. PETRANOVIĆ, Ž. TRGOVČEVIĆ and Vera ZGAGA
THE ACTION OF GAMMA RAYS ON HEAT INDUCTION OF C1 MUTANT OF γ PROPHAGE [Abstract]
Ivka KALAČEVIĆ-KRAJOVAN and Dubravka LUČIĆ
INFLUENCE OF NITROUS ACID AND HYDROXYLAMINE HYDROCHLORIDE ON SACCHAROMYCES CEREVISIAE YEAST CELLS IN DIFFERENT PHASES OF EVOLUTION [Abstract]
D. HRANUELI, Vera JOHANIDES and Marija ALAČEVIĆ
DIFFERENT MUTAGENIC ACTION OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE ON SELECTED CLONES OF CANDIDA LIPOLYTICA [Abstract]
Vera SPURNA and M. NEBOLA
ISOLATION AND CYTOGENETICS OF HYBRIDS DERIVED FROM A CROSS BETWEEN MOUSE LYMPHOSARCOMA AND BIOCHEMICALLY MARKED L-CELL LINES [Abstract]
Milka ŠOPOVA
THE BEHAVIOUR AND TRANSMISSION OF B-CHROMOSOME IN THE POPULATIONS OF ALLIUM FLAVUM L. AND ALLIUM CARINATUM L. FROM MACEDONIA [Abstract]
Dražena Papeš
B CHROMOSOMES OF THE GENUS LEUCANTHEMUM IN YUGOSLAVIA [Abstract]
V. SPASOJEVIĆ
STRUCTURE AND MECHANISM OF THE ACHROMATIC APPARATUS OF MITOSIS [Abstract]
H. D. KLEIN und M. MILUTINOVIĆ
EXPRESSIVITATSSCHWANKUNGEN MUTIERTER GENE IN DER MEIOSIS [Abstract]
M. MILUTINOVIĆ
BEZIEHUNG ZWISCHEN DEM TRANSLOKATIONSGRAD UNO DER CHROMOSOMENVERTEILUNG WAHREND DER MEIOSIS RONTGENINDUZIERTER MUTANTEN [Abstract]
Kosana KONSTANTINOV
FACTORS INFLUENCING THE EFFECT OF ISOPROPYLMETHANESULFONATE AND ETHYLMETHANESULFONATE ON WHEAT AND BARLEY SEEDS [Abstract]
V. SPASOJEVIĆ and Olga BRANKOVAN
CYTOGENETICAL ACTIVITY OF »SEVIN 50« [Abstract]
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 171-179
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
POPULATION GENETIC VARIABILITY IN COLD TEMPERATURE RESISTANCE AT DIFFERENT DEVELOPMENTAL STAGES OF DROSCPHILA MELANOGASTER
N. TUCIĆ
Institut za zoologiju, Prirodno-matematički fakultet, Beograd
Abstract
Tucić, N. (1971): Population-genetic variability in cold temperature resistance at different developmental stages of Drosophila melanogaster. - Genetika, Vol. 3, No. 2, 171-179.
Population-genetic variability in the resistance of Drosophila melanogaster to cold temperatures was measured at five developmental stages. Additive genetic variance was found to be the greatest in adults and among the eggs, and the least among the larvae I. A selection for greater resistance to cold temperatures was made over ten generations at the five developmental stages. A very high selection response was obtained in adults, which increased their cold-resistance by almost five times. The other four developmental stages also increased their cold-resistance, but to a lesser degree.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 181-188
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
SELECTION FOR »NEGATIVE« PHOTOTAXIS IN DROSOPHILA SUBOBSCURA
V. KEKIĆ and D. MARINKOVIĆ
Institut za biološka istraživanja i Prirodno-matematički fakultet, Beograd
Abstract
Kekić, V. and Marinkovic, D. (l971): Selection for »negative« phototaxis in Drosophila subobscura.- Genetika, Vol. 3, No. 2. 181-188.
The distribution of D. subobscura individuals was measured in relation to differences in light intensity in the environment. Using a specially constructed apparatus, five different intensities of light were available, ranging from less than 30 Lux to over 6500 Lux. In order to determine if the characteristic is genetically controlled, selection for the individuals which are most negatively phototactic, was performed. By selecting over four generations the individuals which were distributed where the light intensities were dimmest i.e. at less than 300 Lux, the average phototactic ability was lowered from 4687 to 3252. Heritability for such a complex characteristic was found to be about %%. The results obtained were taken as the basis for a discussion on the relative value of the concept of »negative« or »positive« phototactic ability in insects in general.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 189-203
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
GENETIC STRUCTURE OF POPULATIONS OF TRITICUM AESTIVUM ssp. VULGARE AFTER MUTAGENIC TREATMENTS
Katarina BOROJEVIĆ
Poljoprivredni fakultet, Novi Sad
Abstract
Borojević Katarina (1971): Genetic structure of a populations of Triticum aeslivum ssp. vulgare after mutagenic treatments. - Genetika, Vol. 3, No. 2, 189-203.
In this paper some studies on the genetic structure of populations of Triticum aestivum ssp. vulgare, after different mutagenic treatments are summarized, with special emphasis on changes in population means, genetic variability, and the possibility of selection after mutagenic treatments.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 205-211
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
THE ACTION OF GAMMA RAYS ON HEAT INDUCTION OF C1 MUTANT OF γ PROPHAGE
D. PETRANOVIĆ, Ž. TRGOVČEVIĆ and Vera ZGAGA
Laboratorij za celularnu radiobiologiju, Institut „Rudjer Bošković“, Zagreb
Abstract
Petranović, D., Trgovčević, Ž. and Zgaga Vera (1971): The action of gamma rays on heat induction of a C, mutant of X prophage. - Genetika, Vol. 3, No. 2, 205-211.
Heat induction of the heat inducible prophage γ C1857 is markedly decreased by gamma irradiation. It is proposed that this decrease is due to the prevention of detachment of prophage.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 213-222
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
INFLUENCE OF NITROUS ACID AND HYDROXYLAMINE HYDROCHLORIDE ON SACCHAROMYCES CEREVISIAE YEAST CELLS IN DIFFERENT PHASES OF EVOLUTION
Ivka KALAČEVIĆ-KRAJOVAN and Dubravka LUČIĆ
Tehnološki fakultet, Novi Sad
Abstract
Kalačević-Krajovan Ivka and Lučić Dubravka (1971): Influence of nitrous acid and hydroxylamine hydrochloride on Saccharomyces cerevisiae yeast cells in different phases of evolution. - Genetika, Vol. 3, No. 2, 213-222.
During investigations of some physiological characteristics of Saccharomyces cerevisiae yeast, the yeast cells have been treated with nitrous acid and hydroxylamine. It was observed that: (a) both of the mutants increase the fermentative power of yeast under normal conditions of cultivation; (b) they give the positive Crabtree effect on sucrose; and (c) increase both the fermentative and oxidative power of 96 hours old cells.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 223-235
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
DIFFERENT MUTAGENIC ACTION OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE ON SELECTED CLONES OF CANDIDA LIPOLYTICA
D. HRANUELI, Vera JOHANIDES and Marija ALAČEVIĆ
PLIVA - tvornica farmaceutskih i kemijskih proizvoda, Zagreb
and Tehnološki fakultet, Zagreb
Abstract
Hranueli, D., Johanides Vera and Alačević Mariia (1971): Different mutagenic action of N-methyLN'-Nitro-N-nitrosoguanidine on selected clones of Candida lipolytica. - Genetika, Vol 3, No. 2, 223-235.
MNNG has a different mutagenic effect on the 3 clones of the yeast Candida lipolytica 33 G, 33 N and 33 M at pH 6 and 8. The maximal number of survivors and the maximal number of different mutants were obtained in the clone 33 G at pH 6.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 237-248
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
ISOLATION AND CYTOGENETICS OF HYBRIDS DERIVED FROM A CROSS BETWEEN MOUSE LYMPHOSARCOMA AND BIOCHEMICALLY MARKED L-CELL LINES
Vera SPURNA and M. NEBOLA
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno, Czechoslovakia
Abstract
Spurna Vera and Nebola, M. (1971): Isolation and cytogenetics of hybrids derived from a cross between mouse lympho-sarcoma and biochemically marked L-cell lines. - Genetika. Vol. 3, No. 2, 237-248.
Hybrid cells were prepared from mouse lymphpsarcoma cells with leukemia and from non-malignant L-cell fibroblasts -like resistant to 50 u.g AG/ml (clone R-AG/50-3). For the isolation of hybrid cells a semiselective system was used. Hybrids proliferated well if cultivated in a minimal Eagle's medium supplemented with 10% of calf serum. Among six clones of hybrids differences in generation time, growth capacity, and morphological properties were found. During long-term cultivation of hybrids in vitro the loss of some chromosomes in the new hybrid karyotype was described. Simultaneously, a high suppression of malignancy after the intraperitoneal inoculation of hybrids in two mice of the C 57 BL strain was reported. We suppose, that the tumourgenicity may be suppressed by the cell hybridisation and that it is related to the manifestation of the genome of the original parental malignant cell line in the new hybrid cell genome.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 249-259
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
THE BEHAVIOUR AND TRANSMISSION OF B-CHROMOSOME IN THE POPULATIONS OF ALLIUM FLAVUM L. AND ALLIUM CARINATUM L. FROM MACEDONIA
Milka ŠOPOVA
Department of Botany, Faculty of Science, Skopje, Yugoslavia
Abstract
ŠSopova, Milka (1971): The behaviour and transmission of B-chromosome in the populations of Allium flavum L. and Alli-um carinatum L.-from Macedonia. - Genetika, Vol. 3, No. 2, 249-259.
The cytological behaviour and mode of transmission of B-chromosomes were studied in A. flavum and A. carinatum in natural populations from the mountain Galičica and in material grown under field conditions in the Botanical gardens, Skopje. The cytological data obtained from an examination of B-chromosomes revealed that the frequency of B-chromosomes varied from year to year. Crosses between plants without B-chromosome and plants carrying one B-chromosome (0 x 1 and 1 x 0) were made in order to find out whether the transmission of B-chromosomes was equally expressed through the female and male side. Evidence has been obtained that elimination occurs on the male side to some degree, but in no case has a. mechanism for the numerical increase of B-chromosome been detected. There was some indication too, that the presence of B-chromosomes increased the mean chiasmata fequency in diploid A. flavum and the number of trivalents in A. carinatum.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 261-270
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
B CHROMOSOMES OF THE GENUS LEUCANTHEMUM IN YUGOSLAVIA
Dražena Papeš
Botanical Institute, University of Zagreb, Yugoslavia
Abstract
Papeš Dražena (1971): B chromosomes of the genus Leucanthemum in Yugoslavia. - Genetika, Vol. 3, No. 2, 261-270.
B chromosomes occur in 10 per cent of the Yugoslav populations of different Leucanlhemum species (L. vulgare s. stric-to, L. Icucolepis, L. vulgare subsp. amplifolium, L. croaticum v. illyricum, L. liburnicum v. quarnericum, L. montanum v. adus-turn and L. montanum v. helerophyllum). They have been found at all levels of ploidy, which occur in Yugoslavia (4x, 5x, 6x, 7x and 8x), except in diploids. The morphology of B chromosomes varies, at different ploidy levels, from population to population. There are three types of B chromosomes: very small metacent-rics (in hexaploids L. vulgare subsp. amplifolium and L. monlanum v. adustum), larger metacentrics (in the tetraploid L. leucolepis and in the heptaploid L. sp.), and telocentrics or nearly telocentrics (in octoploids L. liburnicum and L. montanum v. heterophyllum). The number of B chromosomes varies in some populations of tetraploids (from 1—8) and heptaploids (from 1—10), however in pentaploids, hexaploids and octoploids it is very constant. They appear in a high proportion in each population. Venations of B chromosome frequency within and among the populations are the result of different kinds of reproduction (sexual and vegetative).
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 271-283
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
STRUCTURE AND MECHANISM OF THE ACHROMATIC APPARATUS OF MITOSIS
V. SPASOJEVIĆ
Institut za biološka istraživanja, Beograd
Abstract
Spasojević, V. (1971): Structure and mechanism of the achromatic apparatus of mitosis. -Genetika, Vol. 3, No. 2, 271-283.
The air dried seeds of a corn line were treated with ethyl melhanesulfonate and caffeine. Among the other mutants was selected one in which different aberrations of the mitotic spindle were induced. The most frequent forms of spindle aberrations were: longitudinal unilateral or bilateral, symmetrical split of spindle (clear zones in the spindle); disturbance of polarity and development a lunar spindle; tripolar spindle; bifurcation of the spindle and failure of metakinese of one or more chromosomes. Depending upon the type of spindle aberration deformation of cell form appeared too. This mutation was inherited through four generations. The frequency of aberrations in the F1-generation was decreased significantly.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 285-294
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
EXPRESSIVITATSSCHWANKUNGEN MUTIERTER GENE IN DER MEIOSIS
H. D. KLEIN und M. MILUTINOVIĆ
Institut für Genetik Uiniversitat Bonn, BRD und Landwirtschaftliche Fakultat Belgrad - Zemun, Jugoslawien
Abstract
Klein, H. D. und Milutinovic, M. (1971): Expressivitals-schwankungen mutierten Gene in der Meiosis. - Genetika, Vol. 3. No. 2, 285-294.
Es wurden zwei desynaptische Mutanten von Pisum zu zwei unterschiedlichen Fixierungszeitpunkt (26.6. und 8.7.1969) zytogenetisch bearbeitet. Es zeigte sich in beiden Fallen eine deutliche Tendenz zur Erhohung der Gonenzahl je PMZ mit zunehmendem Alter der mutierten Fflanzen. Wahrend die Ursache hierfur bei der Mutante 242AB in einer Zunahme der Univalente in der Metaphaseplatte und einer gesteigerten Haufigkeit von Univalententeilungen in der Anaphase I zu suchen ist, wird beim Genotyp 232 in Verbindung mit einer Abnahme der Univalenten-haufigkeit in der Metaphase I und der durchschnittlichen Spin-delzahl je PMZ in der Anaphase II die Wahrscheinlichkeit fiir die Bdldung vooi Mikronuolei und Mikroganen und somit auch fiir die Gesamtanizahl der Goneai je PMZ grosser.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 295-305
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
BEZIEHUNG ZWISCHEN DEM TRANSLOKATIONSGRAD UNO DER CHROMOSOMENVERTEILUNG WAHREND DER MEIOSIS RONTGENINDUZIERTER MUTANTEN
M. MILUTINOVIĆ
Poljoprivredni fakultet, Beograd — Zemun
Abstract
Milutinović, M. (1971): Bezichung zwischen dem Translokationsgrad und der Chromosomenverteilung, wahrend der Meiosis rontgeninduz.ie.rler Mulanten. - Genetika, Vol.3, No. 2, 295-302.
Es wurden die Beziehungen zwischen dem Translokationsgrad und dem Chromosomenverhalten wahrend der Meiosis unter-sucht. Die Mutanten mit 3 und 7 translozierten Chromosomen zeigen jeweils iiber 20% »laggards« in der Ai und Tl wobei beziig-lich der laggard-Haufigkeit praktisch kein Unterschied zwischen den beiden Formen besteht. Je holier der Translokationsgrad ist desto grosser ist die Anzahl der Fragmente im Zwischenpolbereich und der Chroinosomenbriicken. Die zahlenmassige normale Chro-mosomenverteilung in der Ana- bzw. Telophase I (7 + 7) steht in einer negative Korrelation zum Translokationsgrad.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 303-315
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
FACTORS INFLUENCING THE EFFECT OF ISOPROPYLMETHANESULFONATE AND ETHYLMETHANESULFONATE ON WHEAT AND BARLEY SEEDS
Kosana KONSTANTINOV
Zavod za genetiku, INEP, Zemun
Abstract
Kosana Konstantinov (1971): Factors influencing the effect of isopropylmelhanesulfonate and ethylmethanesulfonate on wheat and barley seeds.- Genetika, Vol. 3, No. 2, 303-315.
This paper deals with comparative studies of the effects of isoPMS and EMS on wheat and barley seeds and their dependence on different factors before and during the treatment. According to our results it can be concluded that the effect of isoPMS on both wheat and barley seeds is dependent on the concentration of mutagen in the solution, the time of presoaking of the seeds and the pH during the treatment.
Published in „GENETIKA“ Vol. 3, No.2 (1971), pp. 317-331
© 1971 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
CYTOGENETICAL ACTIVITY OF »SEVIN 50«
V. SPASOJEVIĆ and Olga BRANKOVAN
Institut za biološka istraživanja, Beograd
Abstract
Spasojević, V. and Brankovan, Olga (1971): Cytogenetical activity of »Sevin 50«. - Genetika, Vol. 3, No. 2, 317-331.
Corn seeds were treated for 24 and 48h in different concentrations of »Sevin 50«. All concentrations applied were active inducing inhibition of germination, a decrease in the mitotic index and the expression of C-mitotic properties. Besides the primary effect after 48h treatment, inactivation of the mitotic spindle was found in slides of all concentrations, so that mitosis was arrested at the metaphase stage. There were more than twenty chromosomes in 20% prophases and 45% metaphases. The abnormalities decreased with the duration of the reparation period.