GENETIKA, Vol. 40, No.2 (2008)
Jelena BOŠKOVIĆ, Momčilo BOŠKOVIĆ and Željana PRIJIĆ
H.K. YADAV, S. SHUKLA and S.P.SINGH
DISCRIMINANT FUNCTION ANALYSIS FOR OPIUM AND SEED YIELD IN OPIUM POPPY (Papaver somniferum L.) [Abstract] [Full text]
Čedomir RADENOVIĆ, Milomir FILIPOVIĆ, Milosav BABIĆ, Goran STANKOVIĆ, Aleksandar RADOJČIĆ, Mile SEČANSKI, Jovan PAVLOV, Dragana BRANKOVIĆ RADOJČIĆ and Dragojlo SELAKOVIĆ
ACTUAL PRESTIGIOUS PROPERTIES OF MAIZE INBRED LINES – A GOOD INITIAL BASIS FOR THE EFFICIENT DEVELOPMENT OF NEW AND YIELDING MAIZE HYBRIDS [Abstract] [Full text]
Nada HladnI, Dragan Škorić and Marija Kraljević-Balalić
LINE X TESTER ANALYSIS OF MORPHOPHYSIOLOGICAL TRAITS AND THEIR CORRELATIONS WITH SEED YIELD AND OIL CONTENT IN SUNFLOWER (Helianthus annuus L.) [Abstract] [Full text]
Jelena VANČETOVIĆ
AN IMPACT OF ENVIRONMENT ON SEGREGATION RATIO OF QUALITATIVE TRAITS IN MAIZE [Abstract] [Full text]
Aleksandar LUČIĆ, Ana NIKOLIĆ , Snežana MLADENOVIĆ DRINIĆ, Vasilije ISAJEV and Vera LAVADINOVIĆ
GENETIC CHARACTERISATION OF GENOTYPES OF AUSTRIAN PINE (Pinus nigra Arnold) POPULATIONS USING PROTEIN MARKERS [Abstract] [Full text]
Jelena SRDIĆ, Ana NIKOLIĆ and Zorica PAJIĆ
ssr MARKERS IN CHARACTERISATION of sweet corn inbred lines [Abstract] [Full text]
Dragana JOŠIĆ, Bogić MILIČIĆ, Snežana MLADENOVIĆ DRINIĆ and Mirjana JARAK
GENODIVERSITY OF DOMINANT Rhizobium leguminosarum bv. trifolii ISOLATED FROM 11 TYPES OF SOIL IN SERBIA [Abstract] [Full text]
Milovan PAVLOV, Goran SARATLIĆ, Živorad VIDENOVIĆ and Zoran STANIŠIĆ
A MODEL OF A SUCCESSFUL UTILISATION OF A HIGH GENETIC POTENTIAL OF MAIZE YIELD [Abstract] [Full text]
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 95-107
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 632.4
Original scientific paper
Jelena BOŠKOVIĆ 1, Momčilo BOŠKOVIĆ 2 and Željana PRIJIĆ 1
Abstract
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 109-120
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575:633.75
Original scientific paper
Discriminant Function Analysis for Opium and Seed Yield in Opium Poppy (Papaver somniferum L.)
H.K. YADAV, S. SHUKLA and S.P.SINGH
Division of Genetics and Plant Breeding, National Botanical Research Institute (CSIR), Lucknow-226001, India.
Abstract
Yadav, H.K., S. Shukla and S.P. Singh (2008). Discriminate function analysis for opium and seed yield in opium poppy (Papaver somniferum L.). – Genetika, Vol. 40, No. 2, 109 – 120.
Opium poppy (Papaver somniferum L.) is an important medicinal plant of pharmacopoel uses. Opium latex and its derivatives are used in different medicines as analgesic, narcotic, sedative, sudorific, hyponitic, antispasmodic, ant diarrhea and cough etc. Genetic improvement in opium and seed yield, component breeding is important and selection based on multiple characters is more beneficial in developing desired plant types. Therefore the present study was made on group of 22 strains of opium poppy to find out variability and suitable selection indices for opium and seed yield. Heritability in broad sense was high for capsule weight/plant, plant height, capsule length, stem diameter and opium yield. The discriminant functions based on single character were less efficient while on the basis of combination it was in general more efficient. The comparison of different functions revealed that capsule weight/plant, capsule length, plant height are major yield component and thus practicing selection for attainment of high opium and seed yield lines, maximum weight age could be given to these characters. The positive association of opium yield and seed yield suggested that by adopting suitable component breeding and selection, a dual-purpose variety (opium and seed yield) may be developed.
Key words: Discriminant, Opium, Relative efficiency index, Selection index, Variability
Corresponding author: S.P. Singh, National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India Tel: 91-522-2205842; Fax:91-522-2205836;
e-mail: sant_psingh@yahoo.com
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 121-133
© 2008Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.15
Original scientific paper
ACTUAL PRESTIGIOUS PROPERTIES OF MAIZE INBRED LINES – A GOOD INITIAL BASIS FOR THE EFFICIENT DEVELOPMENT OF NEW AND YIELDING MAIZE HYBRIDS
Čedomir RADENOVIĆ1,2, Milomir FILIPOVIĆ1, Milosav BABIĆ1, Goran STANKOVIĆ1, Aleksandar RADOJČIĆ1, Mile SEČANSKI1, Jovan PAVLOV1, Dragana BRANKOVIĆ RADOJČIĆ1 and Dragojlo SELAKOVIĆ1
1Maize Research Institute, Zemun Polje, Belgrade, Serbia
2Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia
Abstract
Radenović Č., M.Filipović, M.Babić, G. Stanković, A. Radojčić, M. Sečanski, J. Pavlov, D. Branković Radojčić and D.Selaković (2008): Actual prestigious properties of maize inbred lines – a good initial basis for the efficient development of new and yielding maize hybrids.- Genetika, Vol. 40, No2, 121 - 133.
This study conforms our hypothesis that there are elite maize inbred lines, which can be considered actual and prestigious as they have not only a property of the water status and a greater grain dry down rate during the maturation period, but also a property of the efficient photosynthetic-fluorescence model that is successfully used in the contemporary processes of breeding, and thereby in the development of new and yielding maize hybrids. Presented results obtained on the dynamics of grain dry down during the maturation period and on photosynthetic-fluorescence parameters (temperature dependence of the chlorophyll delayed fluorescence intensity, the Arrhenius plot for the determination of critical temperatures, i.e. phase transition temperatures and the activation energy) show that properties of the observed inbreds are based on effects and nature of conformational and functional changes occurring in their thylakoid membranes and other chemical structures of grain tissues. Summarised results of studies on actual and prestigious properties of maize inbreds will contribute to more exact, rational and expeditious proceedings of contemporary processes of breeding.
Key words: adaptability, chlorophyll delayed fluorescence, drought, grain dry down, high temperature, inbred line, intact leaf, Zea mays L., thylakoid membrane, transport processes.
Corresponding author: Čedomir Radenović, Maize Research Institute, Slobodana Bajica 1, 11185 Belgrade-Zemun, Serbia
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 135-144
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633
Original scientific paper
LINE X TESTER ANALYSIS OF MORPHOPHYSIOLOGICAL TRAITS AND THEIR CORRELATIONS WITH SEED YIELD AND OIL CONTENT IN SUNFLOWER (Helianthus annuus L.)
Nada HladnI1, Dragan Škorić1 and Marija Kraljević-Balalić2
1Institute of Field and Vegetable Crops, Novi Sad
2Faculty of Agriculture, Novi Sad
Abstract
Hladni N., D. Škorić and M. Kraljević- Balalić (2008): Line x tester analysis of morphophysiological traits and their correlations with seed yield and oil content in sunflower (Helianthus annuus L.).– Genetika, Vol. 40, No. 2, 135 - 144.
One of the primary tasks of sunflower breeding is the development of inbred lines by interspecific hybridization for the purpose of obtaining high-yielding, stable hybrids that are characterized by altered plant appearance and the ability to produce a higher number of plants per unit area under intensive agriculture conditions.Studied in this paper by the line x tester method were seven new divergent cms inbred lines (A) lines, three Rf restorers utilized as testers, and 21 F1 hybrids developed.
Significant differences in the mean values of all the traits studied were observed. Highly significant GCA and SCA values were obtained for petiole length (PL) and total leaf area per plant (TLA). The nonadditive component of genetic variance played the main role in the inheritance of both these traits. This was confirmed by the GCA/Sca ratios for PL and LA in the F1 generation, which were below the value of one (0.43 and 0.07, respectively). The greatest average contribution to the expression of PL (49.9%) and TLA (57.1%) was found in the female A lines.
A positive correlation was found between seed yield (SY) and PL (0.374*) and TLA (0.630**), while seed oil content (SOC) and TLA were found to be negatively correlated (-0.520**). The findings of this study can be used in the development of new high-yielding sunflower hybrids with high yields based on interspecific hybridization.
Key words: combining abilities, correlations, gene effects, interspecific hybridization, sunflower
Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Novi Sad1, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 4898411, fax 381 21 413833, e-mail: nadah@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 145-156
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575:633.15
Original scientific paper
AN IMPACT OF ENVIRONMENT ON SEGREGATION RATIO OF QUALITATIVE TRAITS IN MAIZE
Jelena VANČETOVIĆ
Maize Research Institute “Zemun Polje” , Belgrade, Serbia
Abstract
Vančetović J. (2008). An impact of environment on segregation ratio of qualitative traits in maize – Genetika, Vol. 40, No. 2, 145 - 156.
The significant influence of environment was found on the segregation ratio in a dyhibrid inheritance in maize. Two possible causes are proposed for this segregation distortion: 1) environmental influence (selection) prior forming the gametes or/and 2) different preferential pairing in different environments. Further studies, however, on other self or cross-pollinated plant species, and with different traits are needed to better understand this phenomena.
Key words: environment, maize, segregation distortion, qualitative traits
Corresponding author: Jelena Vancetovic, Maize Research Institute “Zemun Polje” , Slobodana Bajica 1, 11080 Zemun-Belgrade Serbia , E-mail: vjelena@mrizp.co.yu
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 157-168
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 630
Original scientific paper
GENETIC CHARACTERISATION OF GENOTYPES OF AUSTRIAN PINE (Pinus nigra Arnold) POPULATIONS USING PROTEIN MARKERS
Aleksandar LUČIĆ1, Ana NIKOLIĆ2 , Snežana MLADENOVIĆ DRINIĆ2,
Vasilije ISAJEV3 and Vera LAVADINOVIĆ 1
1Institute of Forestry, Belgrade
2Maize Research Institute, Zemun Polje, Belgrade
3Faculty of Forestry, Belgrade
Abstract
Lučić A., A. Nikolić, S. Mladenović Drinić, V.Isajev and V. Lavadinović (2008): Genetic characterisation of genotypes of Austrian pine (Pinus nigra Arnold) populations using protein markers. – Genetika, Vol. 40, No. 2, 157- 168.
The occurrence of Austrian pine over large areas attacked by erosion processes or on completely degraded and denuded areas on which it produces excellent results, classifies it among the most important economic species in forestry. The aim of present research is to study intra- and inter-population genetic variation in Austrian pine by the application of biochemical markers. A better knowledge of the genetic potential of Austrian pine populations will enhance the production of seed and planting material, and in this way also the success of afforestation and the establishment of Austrian pine specific-purpose plantations. The polymorphism of protein markers was determined based on the selected genotypes originating from six populations (seed stands), which actually included all Austrian pine populations in Serbia. Based on the derived electrophoregram, qualitative and quantitative differences (number and pattern) in protein fractions were identified and the seed protein profile was constructed for each tree, as well as for each of the six study populations. This showed the “conservativeness“of some polypeptides, i.e. the presence in all analysed genotypes. On the other hand, some protein fractions were variable at the population level, and some were variable depending on the population. Based on the electrophoregram, the coefficient of genetic similarity/distance was calculated by comparing the band numbers and patterns. Cluster analysis dendrograms were also constructed. The degree of genetic variation among the provenances was higher than the variation within the provenances. There was also a high genetic variation in seed proteins within the analysed populations.
Key words: Austrian pine, proteins, polymorphism, seed
Corresponding author: Aleksandar Lučić; Institute of Forestry; Kneza Višeslava 3; 11000 Belgrade; Serbia; e-mail: aleksandar.lucic@gmail.com
Published in „GENETIKA“ Vol. 40, No.2(2008), pp. 169-177
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.15
Original scientific paper
ssr MARKERS IN CHARACTERISATION of sweet
corn inbred lines
Jelena SRDIĆ, Ana NIKOLIĆ and Zorica PAJIĆ
Maize Research Institute, „Zemun Polje“ - Belgrade, Serbia
Abstract
Srdić J., A.Nikolić and Z.Pajić (2008): ssr markers in characterisation of sweet corn inbred lines..– Genetika, Vol. 40, No. 2, 169-177.
Sweet corn differs from field corn in many important traits. So its breeding although includes some standard procedures demand application of techniques that are important for determining special traits, all because of the specificity of its usage. Application of molecular markers becomes almost a necessity for the breeding of sweet corn, especially because this is the type of maize in which still no definitive heterotic patterns have been determined. So getting to know genetic divergence of the sweet corn inbred lines is of great importance for its breeding.
In this paper we analyzed genetic similarity of six sweet corn inbreds based on SSR markers. 40 SSR primers were used in DNA amplification. Results were compared and correlated with the data on specific combining ability, obtained by the diallel analysis.
The results of SCA were in concurrence with genetic similarity. Values of rank correlation coefficient were negative, indicating that more similar inbred lines had smaller estaimates of SCA, and lines that were less simliar had higher estimates of SCA. Rank correlation coefficient between SCA and GS according to Dice coefficient was between -0,16 and -0,57*.
Key word: genetic similarity, SSR, sweet corn, SCA.
Corresponding author: Jelena Srdić, Maize Research Institute, Slobodana Bajića 1, 11080 Zemun – Belgrade, Serbia, phone: #381 11/3756-704, e-mail: jsrdic@mrizp.co.yu
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 179-190
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575:631.427
Original scientific paper
GENODIVERSITY OF DOMINANT Rhizobium leguminosarum bv. trifolii ISOLATED FROM 11 TYPES OF SOIL IN SERBIA
Dragana JOŠIĆ1, Bogić MILIČIĆ1, Snežana MLADENOVIĆ DRINIĆ2 and Mirjana JARAK3
3 Faculty of Agriculture, Novi Sad, Serbia
Abstract
Jošić D., B. Miličić, S. Mladenović Drinić and M. Jarak (2008): Genodiversity of dominant Rhizobium leguminosarum bv. trifolii isolated from 11 types of soil in Serbia. – Genetika, Vol. 40, No. 2, 179-190.
Rhizobium leguminosarum bv. trifolii is microsymbiont Trifolium pratense and Trifolium repens, which are very important legumes in Serbia. The natural nodulating population of those bacteria was collected and estimated biodiversity distribution by monitoring dominant genotypes of these bacteria.
The population of Rhizobium leguminosarum bv. trifolii were colected from 50 marked locations of 11 types of soil in Serbia. 437 natural isolates, rescued from nodules of Trifolium repens or Trifolium pratense, were analysed by phenotypic approache. We obtained 156 different isolates on the basis of differences in their IAR - intrinsic antibiotic resistance (five antibiotics) and HMT- heavy methal tolerance (five heavy metals). We investigated 56 dominant isolates with more than three differences in IAR-HMT patterns by REP-PCR and RAPD fingerprinting (AP10 and SPH 1 primers). The results showed genodiversity of dominant Rhizobium leguminosarum bv. trifolii field isolates and offered the possibility to assess their changes on marked locations during time and under different environmental conditions and geographical distribution.
Key words: genodiversity of Rhizobium leguminosarum bv. trifolii indigenous population Rhizobium leguminosarum bv. trifolii, IAR, HMT RAPD fingerprinting, REP-PCR
Corresponding author: Dragana Jošić, Institute of Soil Science, Teodora Drajzera 7, Belgrade, Serbia,e-mail: josic@infosky.net, tel/ fax: 011 2667 123
Published in „GENETIKA“ Vol. 40, No.2 (2008), pp. 191-203
© 2008Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.15
Original scientific paper
A MODEL OF A SUCCESSFUL UTILISATION OF A HIGH GENETIC POTENTIAL OF MAIZE YIELD
Milovan PAVLOV, Goran SARATLIĆ, Živorad VIDENOVIĆ
and Zoran STANIŠIĆ
Maize Research Institute, Zemun Polje, Belgrade, Serbia
Abstract
Pavlov M., G. Saratlić, Ž. Videnović and Z. Stanišić (2008): A model of a successful utilisation of a high genetic potential of maize yield. – Genetika, Vol. 40, No. 2, 191 - 203.
The principle of a system, defined as a ZP system, implying corresponding relationship among research, seed production and seed marketing, is that each segment within the system has its tasks and responsibilities, as well as, a clear interest. This system was established at the Maize Research Institute, Zemun Polje, almost half a century ago. The crucial characteristic is that this system encompasses obtained results of scientific accomplishments (patent - a released hybrid), optimal utilisation of the environmental conditions, facilities for seed drying, processing and packing, staff and transport capacities. The ZP system provides the economic interest of all participants in studies and the maize seed production.
The fundamental base of the quality seed production within the ZP system is a multidisciplinary programme on maize breeding, as well as, 535 released hybrids with standard and specific traits. According to regulations in foreign countries, approximately 100 ZP maize hybrids have been released abroad.
Agroecological conditions in Serbia are favourable for the development of the best genotypes and the production of basic and certified maize seed. There 10 processing plants that apply recent technologies in the maize seed processing procedure. Several generations of experts have been trained and gained experience within the maize seed production. Three seed testing laboratories have been accredited by the International Seed Testing Association. According to regulations in Serbia, monitoring of seed production under field conditions, and further on, during the processing practice is done only by designate authorities. This study presents one of successful systems of the seed production organisation applicable in countries with similar conditions.
Key word: basic seed, certified seed, genetics and breeding, maize yield, seed processing, seed fractioning, seed fractions, seed packing.
Corresponding author: Živorad Videnović, zvidenović@mrizp.co.yu, Maize Research Institute, Zemun Polje, Belgrade, Serbia, S. Bajića 1, 11185 Zemun Polje, Serbia