GENETIKA, Vol. 41, No. 1 (2009)
Marija KRALJEVIĆ-BALALIĆ, Novica MLADENOV, Igor BALALIĆ and Miroslav ZORIĆ
VARIABILITY OF LEAF CADMIUM CONTENT IN TETRAPLOID AND HEXAPLOID WHEAT [Abstract] [Full text]
Vesna KANDIĆ, Dejan DODIG, Miroslava JOVIĆ, Bogdan NIKOLIĆ, and Slaven PRODANOVIĆ
THE IMPORTANCE OF PHYSIOLOGICAL TRAITS IN WHEAT BREEDING UNDER IRRIGATION AND DROUGHT STRESS [Abstract] [Full text]
Natalija KRAVIĆ, Violeta ANĐELKOVIĆ, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ and Mirjana VULETIĆ
ANTIOXIDANT ACTIVITY IN SEEDS OF MAIZE GENOTYPES WITH DIFFERENT PERCENTAGE OF EXOTIC GERMPLASM [Abstract] [Full text]
Mirjana MIHAILOVIĆ, Melita VIDAKOVIĆ, Nevena GRDOVIĆ, Svetlana DINIĆ, Aleksandra USKOKOVIĆ, Vesna MARTINOVIĆ and Jelena BLAGOJEVIĆ
THE RADIOPROTECTIVE EFFICACY OF THE RAT ACUTE-PHASE PROTEIN ALPHA2-MACROGLOBULIN ON BONE MARROW CELLS [Abstract] [Full text]
Čedomir RADENOVIĆ , Milomir FILIPOVIĆ, Dragojlo SELAKOVIĆ, Mile SEČANSKI, Vojka BABIĆ, Zoran ČAMDŽIJA, Snežana JOVANOVIĆ, Jovan PAVLOV and Milan STEVANOVIĆ
THE PRESTIGIOUS MAIZE INBRED LINES WITH ERECT TOP LEAVES. THE PRIORITY PERFORMANCE OF THE EFFICIENT PHOTOSYNTHETIC MODEL IN BREEDING [Abstract] [Full text]
Milena SIMIĆ, Željko DOLIJANOVIĆ, Radojka MALETIĆ, Milomir FILIPOVIĆ and Nikola GRČIĆ
THE GENOTYPE ROLE IN MAIZE COMPETITIVE ABILITY [Abstract] [Full text]
Vesna ILIĆ, Ilija TOMIĆ, Gordana CVETKOVIĆ, Radmila BOKUN,and Zvonko MAGIĆ
DETECTION OF K-RAS GENE MUTATION IN BRONCHIAL ASPIRATE OF PATIENTS WITH LUNG CARCINOMA [Abstract] [Full text]
Dragana IGNJATOVIĆ-MICIĆ, Ksenija MARKOVIĆ, Danijela RISTIĆ, Snežana MLADENOVIĆ DRINIĆ, Slavica STANKOVIĆ, Vesna LAZIĆ-JANČIĆ, and Miloje DENIĆ
VARIABILITY ANALYSIS OF NORMAL AND opaque2 MAIZE INBRED LINES
[Abstract] [Full text] [Table]
Jelena VANČETOVIĆ, Ljupcho JANKULOSKI, Sofija BOŽINOVIĆ and Dejan DODIG
The EFFECTS of cytoplasmic male sterility and xenia on the chemical composition of maize grain [Abstract] [Full text]
Snežana MLADENOVIĆ DRINIĆ, Danijela RISTIĆ, Slobodanka SREDOJEVIĆ, Vesna DRAGIČEVIĆ, Dragana IGNJATOVIĆ MICIĆ and Nenad DELIĆ
GENETIC VARIATION OF PHYTATE AND IONORGANIC PHOSPHORUS IN MAIZE POPULATION [Abstract] [Full text]
Amarela TERZIC-VIDOJEVIC, Katarina VELJOVIC, Maja TOLINACKI, Milica NIKOLIC, Mihailo OSTOJIC and Ljubisa TOPISIROVIC
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM ARTISANAL ZLATAR CHEESES PRODUCED AT TWO DIFFERENT GEOGRAPHICAL LOCATION
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 1-10
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC: 575:633.11
Original scientific paper
VARIABILITY OF LEAF CADMIUM CONTENT IN TETRAPLOID AND HEXAPLOID WHEAT
Marija KRALJEVIĆ-BALALIĆ1, Novica MLADENOV2, Igor BALALIĆ2
and Miroslav ZORIĆ3
1Faculty of Agriculture, University of Novi Sad, Novi Sad
2Institute of Field and Vegetable Crops, Novi Sad, Serbia
3Institute of Field Crop Science, Faculty of Agriculture, University of Belgrade
Abstract
Cadmium (Cd) is a toxic trace metal pollutant for humans, animals, and plants. It is a heavy metal present in soils from natural and anthropogenic sources. Much of the Cd taken up by plants is retained in the root, but a portion is translocated to the aerial portions of the plant and into the seed. The objective of this research was to determine the variability and diversity of Cd content in the leaves of 30 wheat cultivars with different ploidy level, during two years. Analyses of Cd content (ppm) in the leaves at heading stage were performed with an atomic absorption spectrometer (AAS). Significant differences between the mean values of the genotypes in Çd content were found. Tetraploid wheat genotypes had higher Cd content than hexaploid genotypes. Cd content was predominantly influenced by the year of growing (73%). The influence of genotype on Cd content amounted 16% and the interaction genotype × year 11%. The cluster of the genotypes consists of four groups. In the groups three and four were some of the genotypes (Kalyan Sona, Partizanka and NS Rana 5) with lowest Cd content in the leaves. They could be chosen as parents in the hybridization for lower cadmium concentration.
Key words: Cd content, interaction genotype×year, heading stage, variability, wheat
Corresponding author: Prof. dr Marija Kraljević Balalić, Faculty of Agriculture, University of Novi Sad, Trg D. Obradovića 8, 21000 Novi Sad, Serbia
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 11-20
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
THE IMPORTANCE OF PHYSIOLOGICAL TRAITS IN WHEAT BREEDING UNDER IRRIGATION AND DROUGHT STRESS
Vesna KANDIĆ, Dejan DODIG, Miroslava JOVIĆ, Bogdan NIKOLIĆ,
and Slaven PRODANOVIĆ
1 Maize Research Institute “Zemun Polje“, Belgrade
2 Center for Agricultural and Technological Research, Zaječar
3 Institute for Plant Protection and the Environment, Belgrade
4 Institute of Field Crop Science, Faculty of Agriculture, Belgrade, Serbia
Abstract
Kandić V., D. Dodig, M. Jović, B. Nikolić and S. Prodanović (2009): The importance of physiological traits in wheat breeding under irrigation and drought stress- Genetika, Vol. 41, No. 1, 11 -20.
The correlation analysis and the path coefficient analysis were applied to wheat data set with the objective to determine the effect of five physiological traits (early vigour, early maturity, leaf senescence, flag leaf area and total biomass per plant) on grain yield under irrigation and drought stress conditions. The data set consisted of 100 divergent genotypes tested in four-year field trials. Highly significant correlations were found between grain yield, early vigour and total biomass per plant in both treatments. A highly negative correlation was detected between grain yield and days to flowering, as well as, between grain yield and leaf senescence in both treatments. The path analysis revealed a highly significant direct effect of days to anthesis and total biomass per plant on grain yield. Early vigour, leaf senescence and the flag leaf area had a significant indirect effect on grain yield via days to anthesis and total biomass per plant. Early vigour, early maturity and leaf senescence were found to be suitable for wheat breeding under different moisture regimes. These traits can be evaluated quickly and easily, and thus they can be used for the evaluation of large populations.
Key words: correlation analysis, grain yield, path analysis, physiological traits, wheat
Corresponding author: Vesna Kandić, Maize Research Institute, Zemun olje, Belgrade, Serbia, Phone: 381 11 3756 704, fax 381 11 3756 707
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 21-28
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
ANTIOXIDANT ACTIVITY IN SEEDS OF MAIZE GENOTYPES WITH DIFFERENT PERCENTAGE OF EXOTIC GERMPLASM
Natalija KRAVIĆ1, Violeta ANĐELKOVIĆ1, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ2
and Mirjana VULETIĆ1
1Maize Research Institute Zemun Polje, Zemun Polje, Serbia
2Institute for Multidisciplinary Reseach, Belgrade, Serbia
Abstract
Kravić N., V. Anđelković, V. Hadži-Tašković Šukalović and M. Vuletić (2009): Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm.– Genetika, Vol. 41, No. 1, 21-28.
In order to broaden the genetic base of maize (Zea mays L.) germplasm, it is necessary to integrate exotic materials into adapted breeding materials. The aim of the study was to compare antioxidative systems of two adapted maize inbred lines (A and B) with exotic germplasm, Drought Tolerant Population (DTP), and their backcrosses with DTP (A1, A2 and B1, B2). The content of low-molecular weight antioxidants, proline and phenolics, as well as antioxidant capacity, detected as free radical scavenging activities against DPPH radical, were measured in maize seeds. Proline content in both, embryo and endosperm was higher in backcrosses than in inbred lines and DTP, and increased in embryo by getting higher percentage of exotic germplasm. Contrary, phenolic content and DPPH radical scavenging activity of seeds, which were higher in adapted inbred lines than in DTP, were slightly decreased in their backcrosses with DTP.
Key words: antioxidant activity, proline, phenolics, exotic germplasm, Zea mays L.
Corresponding author: Natalija Kravić, Maize Research Institute Zemun Polje, S. Bajića 1, 11185 Zemun Polje, Serbia, Phone: +381 11 3756 704,Fax: +381 11 3756 707,(E-mail: nkravic@mrizp.rs)
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 29-39
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
THE RADIOPROTECTIVE EFFICACY OF THE RAT ACUTE-PHASE PROTEIN ALPHA2-MACROGLOBULIN ON BONE MARROW CELLS
Mirjana MIHAILOVIĆ, Melita VIDAKOVIĆ, Nevena GRDOVIĆ, Svetlana DINIĆ, Aleksandra USKOKOVIĆ, Vesna MARTINOVIĆ and Jelena BLAGOJEVIĆ
University of Belgrade, Institute for Biological Research “Siniša Stanković”, Department for Molecular Biology, Belgrade, Serbia
Abstract
Mihajlović M., M. Vidaković, N. Grdović, S. Dinić, A. Uskoković, V. Martinović and J.Blagojević (2009): The radioprotective efficacy of the rat acute-phase potein alpha2-macroglobulin on bone marrow cells– Genetika, Vol. 41, No. 1, 29-39.
The rat acute phase protein α2-macroglobulin (α2M) plays an important role in the restoration of disrupted homeostasis by inhibiting different types of non-specific proteases and facilitating the transport of cytokines, growth factors and hormones. Previously, we observed that administration of α2M to experimental animals prior to the infliction of life-threatening trauma in the form of scalding or total-body irradiation, significantly improved their survival rates. The aim of the present work was to evalute the radioprotective effect on blood cells of α2M that, when administered 30 min before irradiation with 6.7 Gy (LD50/30), provides 100% survival of experimental animals where in unprotected irradiated rats the said dose results in 50% lethality. We observed that rats pretreated with α2M, after an initial decline, exhibited complete recovery of the leukocyte count due to the preservation of bone marrow cells, observed as a stable mitotic index. In untreated irradiated rats the decrease of the mitotic index reflected the significant destruction of bone marrow cells that resulted in a protracted decline in the leukocyte count. We conclude that the radioprotection provided by α2M was in part mediated through cytoprotection of new blood cells produced in the bone marrow.
Key words: α2-macroglobulin, total body irradiation, mitotic index, bone marrow
Corresponding author: dr Mirjana Mihailović; Institute for Biological Research, Bulevar despota Stefana 142, 11060, Belgrade, Serbia,e-mail: mista@ibiss.bg.ac.yu; tel: +381 11 2078343; fax: +381 11 2761433
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 41-58
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
THE PRESTIGIOUS MAIZE INBRED LINES WITH ERECT TOP LEAVES. THE PRIORITY PERFORMANCE OF THE EFFICIENT PHOTOSYNTHETIC MODEL IN BREEDING
Čedomir RADENOVIĆ 1,2, Milomir FILIPOVIĆ1, Dragojlo SELAKOVIĆ1, Mile SEČANSKI1, Vojka BABIĆ1, Zoran ČAMDŽIJA1, Snežana JOVANOVIĆ1, Jovan PAVLOV1 and Milan STEVANOVIĆ1
1Maize Research Institute, Zemun Polje, Belgrade, Serbia
2Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia
Abstract
Radenović Č., M. Filipović, D. Selaković, M. Sečanski, V. Babić, Z.Čamdžija, S.Jovanović, J. Pavlov and M. Stevanović (2009): The prestigious maize inbred lines with erect top leaves. the priority performance of the efficient photosynthetic model in breeding– Genetika, Vol. 41, No. 1, 41 - 58.
This study conforms the hypothesis that there are elite maize inbred lines with erect top leaves that have a property of an efficient photosynthetic model and that as such are successfully used in the processes of breeding in which the number of plants is increased per area unit (plant density). This proof was established by the application of non-invasive photosynthetic-fluorescence method suitable for the evaluation of the efficiency of the photosynthetic model. The obtained photosynthetic and fluorescence properties of observed prestigious maize inbred lines with the erect top leaves are based on the effects and the nature of changes in chlorophyll fluorescence occurring in their thylakoid membranes. Their principal parameters are temperature dependence of the chlorophyll delayed fluorescence intensity, the Arrhenius plot for the determination of the phase transition in thylakoid membranes and the estimated activation energies.
The displayed results on the size of an angle between the direction of the propagation of the above-ear leaf and the direction of the stalk propagation, as well as, results on the dynamics of grain dry‑down during the maturation period, additionally indicate that traits of observed maize inbred lines with erect top leaves are the prominent base for more exact, rational and faster proceeding of current processes of breeding.
Key words: adaptability, delayed chlorophyll fluorescence, erect top leaves, grain dry-down, inbred line, intact leaf, photosynthetic model, resistance,thylakoid membrane, thermal processes, transport processes
Corresponding author: Čedomir Radenović, Maize Research Institute “Zemun Polje”, Slobodana Bajica 1, 11080 Zemun-Belgrade Serbia
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 59-67
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633.15
Original scientific paper
THE GENOTYPE ROLE IN MAIZE COMPETITIVE ABILITY
Milena SIMIĆ 1, Željko DOLIJANOVIĆ 2, Radojka MALETIĆ 2, Milomir FILIPOVIĆ 1
and Nikola GRČIĆ 1
1Maize Research Institute, Zemun Polje,
2 Faculty of Agriculture, Belgrade-Zemun
Abstract
Simić M., Ž. Dolijanović, R.Maletić, M.Filipović and N. Grkić (2009): The genotype role in maize competitive ability. – Genetika, Vol. 41, No. 1, 59-67.
Growing competitive crops is an important component of the integrated weed management system, although selection of genotypes with the increased competitive ability is rather difficult. The possibility of reducing weed infestation by increasing the competitive activity of crops by growing genotypes, i.e. hybrids that better "endure" a greater density, depends on the FAO maturity group and morphological properties of a genotype, environmental conditions and the growing regions. The competition between maize and weeds varies over the modified crop arrangement pattern. The greatest weed infestation is recorded in the crops of the sparse-spaced plants, while the height and the weight of weeds are significantly lower in closely-spaced plants. The height, growth intensity in the initial stages, embryo vigour and leaf area of the plant are important for a greater competitive ability, as they are components of competitive effects of crops on weeds.
Weed infestation and maize hybrids yielding were studied in dependence on the hybrids arrangement pattern and the application of herbicides.
Obtained results show statistically significant differences in the weed fresh weight, but only in certain years and in accordance with differences in the height and the leaf area of ZP maize hybrids.
Key word: competition, genotype, maize, weeds
Corresponding author: Milena Simić, Maize Research Institute, Zemun Polje, phone: +381 11 3756 704; E-mail: smilena@mrizp.co.rs
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 69-80
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
DETECTION OF K-RAS GENE MUTATION IN BRONCHIAL ASPIRATE OF PATIENTS WITH LUNG CARCINOMA
Vesna ILIĆ ¹, Ilija TOMIĆ ², Gordana CVETKOVĆ ², Radmila BOKUN ³,
and Zvonko MAGIĆ ¹
Institute for Medical Research¹, Clinic of Pulmology2, Institute of Pathology3, Military Medical Academy, Belgrade, Serbia
Abstract
Ilić V., I. Tomić, G.Cvetković, R.Bokun and Z.Magić (2009): Detection of k-ras gene mutation in bronchial aspirate of patients with lung carcinoma..– Genetika, Vol. 41, No. 1, 69 -80.
Lung cancer belongs to a group of tumors with bad prognosis resulting in limited therapeutic chances. It is the most common cause of cancer deaths and cancer-related deaths worldwide. Only 25-40% of lung cancers are considered resectable when a diagnosis is made and just 20% of patients have a confined disease at the time of surgery. That makes problem of early diagnosis of lung cancer one of the biggest challenges in clinical oncology. Our goal was to determine whether molecular genetic assays could augment conventional clinical and laboratory diagnostic procedures.
Bronchoalveolar aspirate of patients with different histological types and stages of NSCLC were analyzed for presence of K-ras oncogene mutations (codons 12 and 13) and compared with cytological findings in the same samples.
Mutations in codons 12 and 13 of K- and H-ras genes in bronchoalveolar aspirate of 53 patients (pts) were examined by polymerase chain reaction and SSCP analysis.
Mutations in K-ras gene were identified in 18/53 (34%) specimens of bronchoalveolar aspirate, out of which 3/18 were adenocarcinomas, 11/18 squamous cell carcinoma, two were with anaplastic and two patients with chronical lung disease. The same samples were examined for presence of malignant cells by conventional cytological analysis. Normal cytological results were found in 6 samples of patients with malignant tumors out of which K-ras mutations were detected in 4 samples.
A presence of mutated K-ras gene may prove useful as an adjunct to cytological analysis and also could serve as additional criteria for early diagnosis in patients with bronchogenic carcinoma.
Key words: early detection, lung cancer, k-ras gene
Corresponding author: Vesna Ilić, Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 2661-122/ 3608-857, vesnailic@hotmail.com
Published in „GENETIKA“ Vol. 41, No.1 (2009), pp. 81-93
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
VARIABILITY ANALYSIS OF NORMAL AND opaque2 MAIZE INBRED LINES
Dragana IGNJATOVIĆ-MICIĆ, Ksenija MARKOVIĆ, Danijela RISTIĆ, Snežana MLADENOVIĆ DRINIĆ, Slavica STANKOVIĆ, Vesna LAZIĆ-JANČIĆ,
and Miloje DENIĆ
Maize Research Institute „Zemun Polje“
Abstract
Ignjatović V., K. Maraković, D. Ristić, S. Mladenović Drinić, S. Stanković, V. Lazić Jančić and M.Denić (2009): Variability analysis of normal and opaque2 maize inbred lines. – Genetika, Vol. 41, No. 1,81 -93.
Nutritional value of maize is poor due to deficiency of two essential amino acids – tryptophan and lysine. It was shown than opaque2 (o2) mutations can nearly double the lysine and tryptophan content of the endosperm compared with the normal type. Maize Research Institute Gene bank maintains a collection of opaque2 inbred lines developed in the seventies, primarily based on kernel hardness and analytical methods. In order to describe these lines in more detail they were analyzed for tryptophan content and subjected to SSR analysis with opaque2 markers, a marker for endosperm hardness modifier gene and the most significant amino acid modifier markers. Also, a pathogenicity test for inbred lines tolerance to Fusarium spp., which is one of the most important maize pathogens in our region and a causer of maize stalk, root and ear rot, was performed. Differences in tryptophan content between normal and opaque2 lines were significant. All primers used for distinguishing alleles between normal and opaqu2 inbred lines gave positive results. Each genotype gave a specific allelic pattern with amino acid modifier gene primers, without any obvious correspondence to the tryptophan content. Phythopathogenicity test showed on average higher susceptibility to Fusarium graminearum of opaque2 genotypes. These results gave an insight into the applicability of the methods in describing opaque2 lines to be converted into quality protein maize QPM - genotype in which opaque2 has been incorporated along with associated modifiers.
Key word: maize, opaque2, phytophatogenicity test, SSR, tryptophan
Corresponding author: Dragana Ignjatović-Micić, Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade. Serbia, Tel: 011/3756704,Fax: 011/3756707,e-mail: idragana@mrizp.co.rs
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 95-106
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633.15
Original scientific paper
The EFFECTS of cytoplasmic male sterility and xenia on the chemical composition of maize grain
Jelena VANČETOVIĆ¹, Ljupcho JANKULOSKI2, Sofija BOŽINOVIĆ¹
and Dejan DODIG¹
1Maize Research Institute, Zemun Polje, Belgrade-Zemun, Serbia
2Faculty of Agricultural Science and Food, Skopje, Macedonia
Abstract
Vančetović J., Lj.. Jankuloski, S. Božinović and D. Dodig (2009): The effects of cytoplasmic male sterility and xenia on the chemical composition of maize grain – Genetika, Vol. 41, No. 1, 95-106.
Sterile hybrids often outyield their fertile counterparts, especially if pollinated by a genetically unrelated pollinator. The combined effect of cms and xenia is referred to as the Plus-hybrid effect. The objective of this study was to determine the individual, as well as, combining effect of cms and xenia on the maize grain chemical composition. The percent of oil, protein and starch in the grain was also observed.
Two sterile hybrids, their fertile counterparts and five fertile pollinator-hybrids were selected for the studies. The three-replicate trial set up according to the split-plot experimental design was performed at Zemun Polje in 2008.
The obtained results show that the effects of cms on the oil percent was not significant in the studied hybrid ZP 341, while it increased at the significance level of P = 0.1 in the second observed hybrid ZP 360. The effect of this factor on the protein and starch percent was also significant (P = 0.01) in some hybrid combinations. Xenia effects on all three chemical parameters were significant (P = 0.01) in some hybrid combinations. The gained results indicate that the identification of a good combination of two hybrids, in which one would be a sterile female component, and the other a pollinator, would end up not only in the increased yield, but also in the improved maize grain quality.
Key words: citoplasmic male sterility; grain quality; maize; plus hybrid effect; xenia
Corresponding author: Jelena Vančetović, Maize Research Institute, Zemun Polje, Belgrade, Serbia, S. Bajića 1, 11185 Zemun Polje, Serbia
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 107-115
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633.15
Original scientific paper
GENETIC VARIATION OF PHYTATE AND IONORGANIC PHOSPHORUS IN MAIZE POPULATION
Snežana MLADENOVIĆ DRINIĆ, Danijela RISTIĆ, Slobodanka SREDOJEVIĆ, Vesna DRAGIČEVIĆ, Dragana IGNJATOVIĆ MICIĆ and Nenad DELIĆ
Maize Research Institute, Zemun Polje, Belgrade, Serbia
Abstract
Drinić Mladenović S, D. Ristić, S. Sredojević, V.Dragičević, D.Ignjatović Micić and N. Delić (2009): Genetic variation of phytate and ionorganic phosphorus in maize population. – Genetika, Vol. 41, No. 1, 107 -115.
Analysis of 60 maize populations was conducted to identify genotypes that had either low or high concentration of phytate. Genetic variability in seed phytate content was observed, with values ranging from 1,147 to 4, 13 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0, 35 and 1, 29 and averaged 0, 65 g kg-1. Three groups of populations were identified as having low, intermediate and high phytate content. The low phytate concentration was measured in eight, intermediate in 25 and high in 27 populations. Positive correlation was found between phytate and protein. Population 216 had the lowest phytate concentration of 1, 14 gkg-1, and a Pi concentration 40% greater than Pi mean but lower than average protein content. This population will be used for further breeding genotypes with low phytate content and good agronomic traits.
Key words: iongarnic phosphorus, phytate, maize population
Corresponding author: Snežana Mladenović Drinić, Maize Research Institute, Zemun Polje, Belgrade, Serbia, S. Bajića 1, 11185 Zemun Polje, Serbia, email:msnezana@mrizp.rs
Published in „GENETIKA“ Vol. 41, No. 1 (2009), pp. 117-136
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM ARTISANAL ZLATAR CHEESES PRODUCED AT TWO DIFFERENT GEOGRAPHICAL LOCATION
Amarela TERZIC-VIDOJEVIC1, Katarina VELJOVIC1, Maja TOLINACKI1, Milica NIKOLIC1, Mihailo OSTOJIC2 and Ljubisa TOPISIROVIC1
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade,
2Faculty of Agriculture, University of Belgrade, Belgrade-Zemun,
Abstract
Terzić-Vidojević A., K. Veljović, M. Tolinački, M. Nikolić, M. Ostojić and Lj. Toipisirović (2009): Characterization of lactic acid bacteria isolated from artisanal Zlatar cheeses produced at two different geographical location. – Genetika, Vol. 41, No. 1, 117 -136.
Eighty-one strains of lactic acid bacteria (LAB) were isolated from white semi-hard homemade cheese, designated Zlatar BGNV, which was taken from household settled on Northern side of mountain Zlatar. The Zlatar BGNV cheese was manufactured from raw cow’s milk without addition of the starter culture. All isolates of LAB were characterized by phenotypic and genotypic tests. Identification of strains was done by the repetitive extragenic palindromic-polymerase chain reaction (rep-PCR) with (GTG)5 primer and 16S rDNA sequence analysis. The most present species in Zlatar BGNV cheese were Lactobacillus casei/paracasei (65.43%) and Enterococcus faecalis (29.63%). Two facultative heterofermentative rods were identified as Lactobacillus plantarum (2.47%), and two obligate hetrofermentative LAB isolates as Lactobacillus parabuchneri (2.47%). Among all 81 tested isolates, only eight enterococci were producers of antimicrobial compounds. Fourteen of 16 tested lactobacilli isolates showed medium to very good proteolytic activity. All 57 lactobacilli from the Zlatar BGNV cheese curdled milk very slowly or did not curdle milk at all. However, three isolates of enterococci, BGNV1-63, BGNV1-76 and BGNV1-80, showed very good activity in milk and curdled milk within 5 h. They showed very high proteolytic activity hydrolyzing completely αs1- and κ-casein after 3 h, and β-casein after 30 min of incubation. In addition, those three enterococcal isolates degraded gelatin. Comparing obtained results with those previously achieved in examination of LAB microflora in another Zlatar BGZLS cheese made also from raw cow’s milk, it can be concluded that LAB microflora in the Zlatar BGNV cheese is less diverse.
Key words: Zlatar cheese, lactic acid bacteria, proteolytic activity, rep-PCR, 16S rDNA
Corresponding author: Amarela Terzić Vidojević, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Vojvode Stepe 444a, P.O.Box 23, 11010 Belgrade, Serbia.Telephone: +381 11 397 59 60; Fax: +381 11 397 58 08;
E-mail: lab6@EUnet.rs