GENETIKA, Vol. 43, No. 2 (2011)
Bogoljub ZEČEVIĆ, Radiša ĐORĐEVIĆ, Ahmet BALKAYA, Jelena DAMNJANOVIĆ, Mladen ĐORĐEVIĆand Ana VUJOŠEVIĆ
INFLUENCE OF PARENTAL GERMPLASM FOR FRUIT CHARACTERS IN F1, F2 AND F3 GENERATIONS OF PEPPER (Capsicum annuum L.) [Abstract] [Full text]
Svetlana BALEŠEVIĆ-TUBIĆ, Vuk ĐORĐEVIĆ, Jegor MILADINOVIĆ,Vojin ĐUKIĆ,and Mladen TATIĆ
STABILITY OF SOYBEAN SEED COMPOSITION [Abstract] [Full text]
Gordana DOZET, Jelena BOŠKOVIĆ, Tamara GALONJA COGHILL,Veselinka ZEČEVIĆ, Gorica CVIJANOVIĆ, Dragan JOVIĆEVIĆ and Vojin ĐUKIĆ
EFFECT OF GENOTYPE AND PRE-SOWING FERTILIZATION ON YIELD OF GARDEN PEA [Abstract] [Full text]
Dursun EŞIYOK, M. KADRI BOZOKALFA,Tansel KAYGISIZ AŞÇIOĞUL
VARIABILITY, HERITABILITY AND ASSOCIATION ANALYSIS IN PLANT TRAITS OF SWISS CHARD (Beta vulgaris subsp. cicla) [Abstract] [Full text] [Full text (page 247)] [Full text (page 248)]
Jela IKANOVIC, Djordje GLAMOCLIJA, Radojka MALETIC, Vera POPOVIC, Dejan SOKOLOVIĆ, Marija SPASIC, and Sveto RAKIC
Path analysis of the productive TRAITs IN sorghum species [Abstract] [Full text]
Ana NIKOLIĆ, Violeta ANDJELKOVIĆ, Dejan DODIG, and Dragana IGNJATOVIĆ-MICIĆ
QUANTITATIVE TRAIT LOCI FOR YIELD AND MORPHOLOGICAL TRAITS IN MAIZE UNDER DROUGHT STRESS [Abstract] [Full text] [Full text (page 268)]
Jan BOĆANSKI, Aleksandra NASTASIĆ, Dušan STANISAVLJEVIĆ, Zorana SREĆKOV, Bojan MITROVIĆ, Sanja TRESKIĆ, Mirjana VUKOSAVLJEV
BIPLOT ANALYSIS OF DIALLEL CROSSES OF NS MAIZE INBRED LINES [Abstract] [Full text]
Dragan LONČAR
CREDIBILITY OF THE COMBINED TEST IN PRENATAL DIAGNOSTICS [Abstract] [Full text]
Nada Hladni, Dragan Škorić, Marija Kraljević-Balalić, Siniša Jocić, and Nenad Dušanić
Line x tester analysis for yield components in sunflower and THEIR CORRELATIONS WITH SEED YIELD (Helianthus annuus L.) [Abstract] [Full text]
Branislav KOVAČEVIĆ, Sasa ORLOVIĆ, Mile IVANOVIĆ, Katarina ČOBANOVIĆ, Emilija NIKOLIĆ-ĐORIĆ, Marina KATANIĆ, and Vladislava GALOVIĆ
RELATIONSHIP AMONG EASTERN COTTONWOOD GENOTYPES ACCORDING TO EARLY ROOTING TRAITS [Abstract] [Full text]
Víctor M. SALCEDA
RELATIVE FREQUENCIES OF THE “SEX-RATIO” INVERSION IN NATURAL POPULATIONS OF Drosophila pseudoobscura FROM MEXICO [Abstract] [Full text]
Evica MRATINIĆ,and Milica FOTIRIĆ AKŠIĆ
EVALUATION OF PHENOTYPIC DIVERSITY OF APPLE (Malus sp.) GERMPLASM THROUGH THE PRINCIPLE COMPONENT ANALYSIS [Abstract] [Full text]
Jelena Srdić, Zorica Pajić, Milomir Filipović, Mliosav Babić, and Mile sečanski
INHERITANCE OF EAR YIELD AND ITS COMPONENTS IN SWEET CORN (Zea mays L. saccharat) [Abstract] [Full text]
Novo PRŽULJ, and Vojislava MOMČILOVIĆ
CHARACTERIZATION OF VEGETATIVE AND GRAIN FILLING PERIODS OF WINTER WHEAT BY STEPWISE REGRESSION PROCEDURE. I. VEGETATIVE PERIOD [Abstract] [Full text]
Svetlana VOJVODIĆ and Dušica ADEMOVIĆ-SAZDANIĆ
HLA CLASS II ANTIGENS AND HAPLOTYPES ASSOCIATED WITH SUSCEPTIBILITY OF LEUKEMIAS AND MYELODYSPLASTIC SYNDROME [Abstract] [Full text]
Valentina DJORDJEVIC, Iva PRUNER, Ljiljana RAKICEVIC, Mirjana KOVAC, Danijela MIKOVIC, Predrag MILJIC, Nebojsa ANTONIJEVIC, and Dragica RADOJKOVIC
FV LEIDEN, FII G20210A AND MTHFR C677T MUTATIONS IN PATIENTS WITH LOWER OR UPPER LIMB DEEP VEIN THROMBOSIS [Abstract] [Full text]
Slađana ŽILIĆ, Dejan DODIG, Marija MILAŠINOVIĆ ŠEREMEŠIĆ,Vesna KANDIĆ, Marija KOSTADINOVIĆ, Slaven PRODANOVIĆ and Đorđe SAVIĆ
SMALL GRAIN CEREALS COMPARED FOR DIETARY FIBRE AND PROTEIN CONTENTS [Abstract] [Full text]
Sanja RADIČEVIĆ, Radosav CEROVIĆ, Slađana MARIĆ, and Milena ĐORĐEVIĆ
FLOWERING TIME AND INCOMPATIBILITY GROUPS – CULTIVAR COMBINATION IN COMMERCIAL SWEET CHERRY (Prunus avium L.) ORCHARDS [Abstract] [Full text]
Vera LAVADINOVIĆ, Vasilije ISAJEV, Zoran MILETIĆ, and Milun KRSTIĆ
VARIABILITY OF NITROGEN CONTENT IN THE NEEDLES OF DOUGLAS-FIR (Pseudotsuga menziesii Mir / Franco) PROVENANCES [Abstract] [Full text]
Biljana GORJANOVIĆ, Milka BRDAR-JOKANOVIĆ, and Marija KRALJEVIĆ-BALALIĆ
PHENOTYPIC VARIABILITY OF BREAD WHEAT GENOTYPES FOR NITROGEN HARVEST INDEX [Abstract] [Full text]
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 209-216
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.842
Original scientific paper
INFLUENCE OF PARENTAL GERMPLASM FOR FRUIT CHARACTERS IN F1, F2 AND F3 GENERATIONS OF PEPPER (Capsicum annuum L.)
Bogoljub ZEČEVIĆ, Radiša ĐORĐEVIĆ, Ahmet BALKAYA,
Jelena DAMNJANOVIĆ, Mladen ĐORĐEVIĆ and Ana VUJOŠEVIĆ
1Institute for Vegetable Crops, Smederevska Palanka, Serbia
2Ondokuz Mayis University, Faculty of Agriculture. Kurupelit – Samsun, Turkey
3Faculty of Agriculture Belgrade – Zemun
Abstract
Zečević B., R. Đorđević, A. Balkaya, J. Damnjanović, M. Đorđević and A. Vujošević (2011): Influence of parental germplasm for fruit characters in F1, F2 and F3 generations of pepper (Capsicum annuum L.). - Genetika, Vol 43, No. 2, 209 -216.
The aim of this research was to determine genetic values for the fruit width and fruit length in F1 and F2 generation of diallely crossed pepper genotypes, and to evaluate the effects of applying the pedigree and bulk breeding method for producing F3 hybrid populations. Hybrid populations were formed by crossing divergent genotypes belonging to different varieties of species Capsicum annuum L. The research of parental lines lasted for three years, experiments with F1 generation two years, while the analysis of results for F3 hybrid population has been done regarding the data from one year experiment. The heterosis effect in F1 generation for all researched traits and in all crossing combinations was very low. Genetic analysis of F2 generation showed that all researched traits were inherited with partial domination. In F3 generation, hybrid populations formed by using the pedigree method of selection had traits which are more valuable for further breeding than the hybrid population formed by using modified bulk method.
Key words: bulk method, genetic analysis, pedigree method, pepper
Corresponding author: Bogoljub Zečević, Institute for Vegetable Crops, Karađorđeva 71, 11420 Smederevska Palanka; tel.: 026317170, fax: 026317785;e-mail: bogoljub.zecevic@gmail.com
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 217 -227
© 2011 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.34
Original scientific paper
STABILITY OF SOYBEAN SEED COMPOSITION
Svetlana BALEŠEVIĆ-TUBIĆ, Vuk ĐORĐEVIĆ, Jegor MILADINOVIĆ,
Vojin ĐUKIĆ, and Mladen TATIĆ
Institute of field and vegetable crops, Novi Sad, Serbia
Abstract
Balešević-Tubić S., V. Đorđević, J. Miladinović, V. Đukić, J. Miladinović, V.Đukić, and M. Tatić (2011): Stability of soybean seed composition. - Genetika, Vol 43, No.2, 217 -227.
Stability of protein and oil content of 13 soybean varieties were examined using linear model across seven locations and during six years. Due to heterogeneity of environments all year/locations was distributed in to two groups, based on achieved yield. Stability of protein content differed in the low and high yielding environments, while average values show only minor differences. In contrast, oil content stability was slightly changed in the low and high yielding environments, while the average oil content were significantly higher in the low yielding environments. Environmental factors influenced the correlation between oil and protein content in soybeans. Negative correlation between protein and oil content was observed only in the high yielding environments, while varieties in low yielding environments lack this well known inverse relation.
Key words: protein content, oil content, soybean, stability, yield
Corresponding author: Svetlana Balešević Tubić, Institute of field and vegetable crops, Maksima Gorkog 30, 21000 Novi Sad. Phone/fax +381214898488, e-mail: svetlana.tubic@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 43, No. 2 (2011), pp. 229 - 238
© 2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635.656
Original scientific paper
EFFECT OF GENOTYPE AND PRE-SOWING FERTILIZATION
ON YIELD OF GARDEN PEA
Gordana DOZET1, Jelena BOŠKOVIĆ1, Tamara GALONJA COGHILL1,
Veselinka ZEČEVIĆ1, Gorica CVIJANOVIĆ1, Dragan JOVIĆEVIĆ2
and Vojin ĐUKIĆ2
1Megatrend University Belgrade, Faculty of Biofarming, Bačka Topola, Serbia,
2Institut for field and vegetable crops, Novi Sad, Serbia
Abstract
Dozet G., J. Bošković, T. Galonja Coghill, V. Zečević, G. Cvijanović, D. Jovićević and V. Đukić (2010): Effect of genotype and presowing fertilization on yield of garden pea.- Genetika, Vol 43, No. 2, 229 -238.
During the two-year study on the influence of presowing fertilization on growth and development of garden pea (Pisum sativum L.) in three different experimental setups, five genotypes of peas were used, two of Dutch origin and three that were created at the Institute of Field and Vegetable Crops.The aim of the study was to determine the extent to which different genotypes and complex NPK fertilizer influence the yield per plant. The average yield per plant for both years was 16.86 g. The influence of genotype on grain yield per plant was statistically significant (p<0.01). Tamish cultivar showed significantly lower yields during both study years, in comparison with other genotypes tested, except when compared with the Danube cultivar in year 2007. There was no regularity in the influence of pre-sowing fertilization on grain yield per plant. Regression analysis of the pea genotypes, revealed a slight reduction in yield per plant as a function of increased use of complex fertilizer. Grain yield per plant was in high positive correlation (p<0.01) with yield components, and with the length of the stem. The number of pods was in strong correlation with grain numbers and weight of pods. Number of grains and the absolute weight were in highly statistically significant correlation with the mass of pods.
Key words: garden pea, genotype fertilization, vegetable, yield
Corresponding author: Gordana Dozet, Megatrend University Belgrade, Faculty of Biofarming, Maršala Tita 39, Bačka Topola, Serbia, phone + 381 24 718515, e-mail: gdozet@biofarming.edu.rs
Published in „GENETIKA“ Vol. 43, No.2 (2011), pp. 239 -252
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC575:633.63
Original scientific paper
VARIABILITY, HERITABILITY AND ASSOCIATION ANALYSIS
IN PLANT TRAITS OF SWISS CHARD (Beta vulgaris subsp. cicla)
Dursun EŞIYOK, M. KADRI BOZOKALFA,Tansel KAYGISIZ AŞÇIOĞUL
Department of Horticulture, Faculty of Agriculture, Ege University, İzmir, Turkey
Abstract
Eşiyok D., M. Kadri Bozokalfa and T. Kaygısız Aşçıoğul (2011): Variability, heritability and association analysis in plant traits of swiss chard (Beta vulgaris subsp. cicla) - Genetika, Vol 43, No. 2, 239-252.
Plant breeders mainly interested in improvement agronomic properties of desirable trait accompanied with genetic architecture of major characters and their interrelationship. Genetic variability and character association are prime importance and provide essential contribution for successful breeding programme. In the present research genetic variability, heritability, phenotypic and genotypic correlations of several agronomic characters were studied in fifty-four swiss chard accessions and cultivars. Analysis of variance observed highly significant variations among accessions, and phenotypic coefficient of variation (PCV) was higher magnitude of genotypic coefficient of variation (GCV) for all agronomic characters. The lamina length, lamina width, petiole thickness, petiole width, lightness, chroma and leaf dry matter observed high magnitude broad sense heritable characters accompanied with high genetic advance. Genotypic correlation coefficient showed higher than phenotypic correlation coefficient which could be explained by low environmental effect on investigated agronomic traits. Positive correlation was revealed between petiole length, lamina length, lamina width, petiole thickness and petiole width which comprise total yield in swiss chard.
Key words: heritability, genetic advance, Swiss chard, variability, quantitative characters
Corresponding author: M. Kadri Bozokalfa, Department of Horticulture, Faculty of Agriculture, Ege University, Bornova 35100 İzmir, Turkey, e-mail: mehmet.kadri.bozokalfa@ege.edu.tr
Published in „GENETIKA“ Vol. 43, No. 2 (2011), pp. 253 -262
© 2011 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
Path analysis of the productive TRAITs
IN sorghum species
Jela IKANOVIC1, Djordje GLAMOCLIJA1, Radojka MALETIC1, Vera POPOVIC2, Dejan SOKOLOVIĆ3, Marija SPASIC1, and Sveto RAKIC1
1Faculty of Agriculture, Belgrade, Serbia
2 Institute of Field and Vegetable Crops, Novi Sad, Serbia
3 Institute for Forage Crops, Krusevac, Serbia
Abstract
Ikanovic J., DJ. Glamoclija, R. Maletic, V. Popovic, D. Sokolovic, M. Spasic, and S. Rakic (2011): Path analysis of the productive traits in Sorghum species.- Genetika, Vol 43, No. 2, 253 -262.
This research studied the phenotypic correlation coefficients between three Sorghum species, namely forage sorghum S. bicolor Moench. (c. NS-Džin), Sudan grass S. sudanense L. (c. Zora) and interspecies hybrid S. bicolor x S. sudanense (c. Siloking). The analyses were performed on plant material samples taken from the first cutting, when plants were in the beginning phase of tasseling. The following morphologic traits were studied: plant height, number of leaves per plant, stem leaf weight and mean stem weight. Additionally, their direct and indirect effect on dependent variable green biomass yield was analysed, for which path coefficients were calculated. This method enables more quality and full insight into relations existing among the studied traits, more precise establishment of cause-effect connections among them, as well as to separate direct from indirect effects of any particular trait on dependent variable, being biomass yield in this case. The analysis of phenotypic coefficients revealed differences in direct and indirect effect of certain traits on dependent variable. Sudan grass had the highest stem (2.281 m) and most leaves per plant (7.917). Forage sorghum had the largest leaf weight per plant (49.05 g), while interspecies hybrid had the highest mean stem weight (80.798 g). Variations of these morphologic traits among species were found to be significant and very significant.
Morphologic traits - stem height and weight significantly affected sorghum green biomass yield. Leaf number and leaf portion in total biomass were negatively correlated with yield. Cultivars differed significantly regarding morphologic and productive traits. Sudan grass had the lowest green biomass yield, while forage sorghum and interspecies hybrid had significant yield increase.
Key words: cultivar, forage sorghum, interspecies hybrid, path analysis of phenotypic coefficients, Sudan grass.
Corresponding author: Jela Ikanovic, Faculty of Agriculture, Belgrade,tel: +381 11 2615 315; e-mail: jela@agif.bg.ac.rs
Published in „GENETIKA“ Vol. 43, No. 2 (2011), pp. 263 -276
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
QUANTITATIVE TRAIT LOCI FOR YIELD AND MORPHOLOGICAL TRAITS IN MAIZE UNDER DROUGHT STRESS
Ana NIKOLIĆ, Violeta ANDJELKOVIĆ, Dejan DODIG,
and Dragana IGNJATOVIĆ-MICIĆ
Maize Research Institute „Zemun Polje“,Belgrade, Srbija
Abstract
Nikolić A., V. Anđelković, D. Dodig, and D.Ignjatović Micić (2011): Quantitative trait loci for yield and morphological traits in maize under drought stress.- Genetika, Vol 43, No. 2, 263 - 276.
Drought is one of the most important factors contributing to crop yield loss. In order to develop maize varieties with drought tolerance, it is necessary to explore the genetic basis. Mapping quantitative trait loci (QTL) that control the yield and associate agronomic traits is one way of understanding drought genetics. QTLs associated with grain yield (GY), leaf width (LW3, LW4) plant height (PH), ear height (EH), leaf number (NL), tassel branch number (TBN) and tassel length (TL) were studied with composite interval mapping. A total of 43 QTLs were detected, distributed on all chromosomes, except chromosome 9. Phenotypic variability determined for the identified QTLs for all the traits was in the range from 20.99 to 87.24%. Mapping analysis identified genomic regions associated with two traits in a manner that was consistent with phenotypic correlation among traits, supporting either pleiotropy or tight linkage among QTLs.
Key words: drought, grain yield, maize, morphological traits, QTLs.
Corresponding author: Ana Nikolić, Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade, Serbia,e-mail: anikolic@mrizp.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 277 -284
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
BIPLOT ANALYSIS OF DIALLEL CROSSES OF NS MAIZE INBRED LINES
Jan BOĆANSKI1, Aleksandra NASTASIĆ2, Dušan STANISAVLJEVIĆ2, Zorana SREĆKOV1, Bojan MITROVIĆ2, Sanja TRESKIĆ2, Mirjana VUKOSAVLJEV3
1 Faculty of Agriculture, Novi Sad, Serbia
2 Institute of Field and Vegetable Crops, Novi Sad, Serbia
3WUR, Wageningen, Netherlands
Abstract
Bočanski J., A. Nastasić, D. Stanisavljević, Z.Srećkov, B.Mitrović, S.Treskić and M. Vukosavljev (2011): Biplot analysis of diallel crosses of NS maize inbred lines- Genetika, Vol 43, No. 2, 277 – 284.
Genetic markers, from morphological to molecular, in function with early Heterosis is a prerequisite for the successful commercial maize production. It does not appear in any cross of two inbred lines, and therefore, the determination of combining abilities of parental lines is essential. The most commonly used method for determining combining abilities is diallel analysis. Besides conventional methods for diallel analysis, a new biplot approach has been sugested. In this paper, we studied the combining ability for grain yield in a set of genotypes obtained by diallel crossing system of six inbred lines. Both, the Griffing’s conventional method and the biplot approach have been used for diallel analysis. Comparing the GCA values from biplot analysis and Griffing’s method, similar results can be observed, with the exception of NS L 1051 and NS L 1000 whose ranks are interchanged. Biplot analysis enables the SCA estimation of parent inbred, and the highest SCA has inbred B73D. Biplot analysis also allows the estimation of the best crosses. Inbred B73D shows the best results when crossed with testers Mo17Ht, NS L 1051 and N152, inbred N152 combines best with testers NS L 1001 and NS L 1000, whereas the cross of inbred NS L 1051 with tester B73D results with the highest grain yield per plant in comparison with other testers.
Key words: biplot, diallel, grain yield,maize
Corresponding author:Jan Boćanski, Faculty of Agriculture, Novi Sad, Serbia
Published in „GENETIKA“ Vol. 43, No.2(2011), pp. 285 -296
©
2010 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
CREDIBILITY OF THE COMBINED TEST IN PRENATAL DIAGNOSTICS
Dragan LONČAR
Gynecology and Obstetrics Clinic, Clinical Center Kragujevac, Kragujevac, Serbia
Abstract
Lončar D. (2011): Credibility of the combined test in prenatal diagnostics- Genetika, Vol 43, No. 2, 285 - 296.
Congenital anomalies are the cause of perinatal death in 20-25% of the cases, while 3% of children are born with malformation of varying size. The objective of this study was to examine the predictive value and define the credibility ratio of the combined test results. Of 317 examined pregnant women, we had sixteen (5.05%) with the result of pathological karyotype after amniocentesis including: nine (2.84%) with fetal numerical aberrations and seven (2.21%) with fetal structural aberrations. While determining the ultrasonographic parameters of the combined test we used the standards of the Fetal Medicine Foundation. We carried out the quantitative settings of free β-HCG and PAPP-A from vein blood of patients by applying commercial tests of firm DPC-USA. Tests were based on the analytical immunochemiluminescence assay and were realized by using the automated analyzer IMMULITE 2000. Manufacturer of the analyzer is also the firm DPC-USA. Sensitivity of the test is 94%, and specificity is 99%. Positive likelihood ratio [likelihood ratio test (LR +)] is 94.00, a negative likelihood ratio is [likelihood ratio test (LR-)] 12:06. Pretest probability that pregnant women carries fetus with chromosomal abnormality is 1:250 or 0004. Posttest odds after the combined test to discover this abnormality is 0.3760, and probability of the same case is 0.2732 if it happens that the test result is positive. The result of our study confirms the justification of combined test usage in routine clinical practice, since the posttest odds rate in the case of a positive screening increases several times over (almost 90 times), the probability of detecting a chromosomal abnormality was about 70 times. Combined screening test if used methodologically correct, has a high predictive value in detecting fetal congenital anomalies.
Key words: family, migraine, recurrent headache.
Corresponding author: Dragan Lončar,Gynecology and Obstetrics Clinic, Clinical Center Kragujevac,Vojislava Kalanovića 1A /3, 34000 Kragujevac, SerbiaPhone: +381 64 616 8999; fax: + 38134365424,email: drloncar@sezampro.rs
Published in „GENETIKA“ Vol. 43, No.2 (2011), pp. 297-306
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
Line x tester analysis for yield components in sunflower and THEIR CORRELATIONS WITH SEED YIELD (Helianthus annuus L.)
Nada Hladni1, Dragan Škorić2, Marija Kraljević-Balalić3,
Siniša Jocić1, and Nenad Dušanić1
1Institute of Field and Vegetable Crops, Novi Sad, Serbia
2Serbian Academy of Science and Arts, Branch in Novi Sad, Serbia
3Faculty of Agriculture, Novi Sad, Serbia
Abstract
Hladni N., D. Škorić, M.Kraljević-Balalić, S. Jocić, V.Miklič,and N. Dušanić (2011): Line x tester analysis for yield components in sunflower and their correlations with seed yield (Helianthus annuus L.).- Genetika, Vol 43, No. 2, 297 - 306.
The creation of new sunflower hybrids with high genetic potential for seed yield on the basis of interspecies hybridization requires a possession of the information about the mode of inheritance and the combining abilities of the created inbreed lines for the total seed number per head and the mass of 100 seeds. Apart from this the research of interdependence between yield components and seed yield allows the defining of traits which have the biggest influence on the yield formation. Significant differences were found among the A lines, Rf testers and their F1 hybrids in total seed number per head and the mass of 100 seeds. Analysis of variance of the combining abilities revealed highly significant differences for GCA and SCA. Highly significant positive value GCA was demonstrated by A-line NS-GS-5 for both traits. Hybrid NS-GS-5xRHA-R-PL-2/1 has the highest significant value SCA for both traits. The main role in inheritance of the total seed number per head and the mass of 100 seeds is played by non additive component of the genetic variance which is confirmed by the GCA/SCA relation in F1 generation that is less than one (0.11, 0.24). Rf-testers had the highest average contribution in the expression of the total seed number per head (55.8%) while for the mass of 100 seeds a more significant contribution was given by A-line (70.6%). A strong positive interdependence has been determined between seed yield and total seed number per head (0.376*) and a very strong positive interdependence was determined between seed yield and the mass of 100 seeds (0.823**). These research can be significant for the creation of new high-yielding sunflower hybrids on the basis of interspecies hybridization.
Key words: combining abilities, correlation gene effect, sunflower, yield components
Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, e-mail: nada.hladni@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 43, No. 2 (2011), pp. 307 - 320
© 2011 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
RELATIONSHIP AMONG EASTERN COTTONWOOD GENOTYPES ACCORDING
TO EARLY ROOTING TRAITS
Branislav KOVAČEVIĆ1, Sasa ORLOVIĆ1, Mile IVANOVIĆ2,
Katarina ČOBANOVIĆ3, Emilija NIKOLIĆ-ĐORIĆ3, Marina KATANIĆ1,
and Vladislava GALOVIĆ1
1Institute for lowland forestry and environment, Novi Sad, Serbia
2Institute for field and vegetable crops, Novi Sad, Serbia
3Faculty of agriculture, Novi Sad, Serbia
Abstract
Kovačević B., S.Orlović., M.Ivanović, K. Čobanović, E. Nikolić-Đorić, M.Katanić and V.Galović (2011): Relationship among eastern cottonwood genotypes according to early rooting traits- Genetika, Vol 43, No. 2, 307 -320.
The relationship between twelve genotypes of eastern cottonwood (Populus deltoides Bartr.) was analyzed according to sixteen early rooting traits and cutting survival. Principal component analysis (PCA) and cluster analysis were used on data that were standardized by common and by one alternative way of standardization. Alternative way of standardization (standardization with within-genotype standard deviation instead of standard deviation of genotypes’ means) was used in order to emphasize the contribution of genotype to the effect of differences among genotypes on total variation. After bought ways the first principal component had high correlation with the most of rooting traits and cutting survival, while the second was mainly related to the traits of root formation on the basal cut of cutting (wound roots). Three difficult-to-root genotypes (S6-7, S1-3, 129/81) were distinctly grouped against other examined genotypes, by bought principal component and cluster analysis. There was a slight difference in grouping of easy-to-root genotypes (B-229 and PE19/66) among examined ways of standardization.
Key words: multivariate analysis, Populus deltoides, root and shoot development
Corresponding author: Branislav Kovačević,Institute for lowland forestry and environment University of Novi Sad, Antona Čehova 13,21000 Novi Sad,Serbia,email: branek@uns.ac.rs,
tel./fFax +381 540 383
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 321 - 329
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
RELATIVE FREQUENCIES OF THE “SEX-RATIO” INVERSION IN NATURAL POPULATIONS OF Drosophila pseudoobscura FROM MEXICO
Víctor M. SALCEDA
Departamento de Biología. Instituto Nacional de Investigaciones Nucleares,México
Abstract
Salceda M.V. (2011): Relative frequencies of the “sex-ratio” inversion in natural populations of Drosophila pseudoobscura from Mexico. - Genetika, Vol 43, No. 2, 321 - 329.
Sex-ratio (proportion of males) in a species is related to a not entirely explained mechanism of sex determination. In Drosophila, sex is determined by the proportion of X chromosomes vs. autosomes. A decrease in the proportion of males vs. females, known as sex-ratio, is characteristic to several Drosophila species, and is related to an inversion in the sexual chromosome the so called “sex-ratio” (SR) condition. In this occasion we study the presence of that inversion in several populations of Drosophila pseudoobscura from Mexico. With this purpose we did collections of this species on Nevado de Colima,Col., Valparaíso, Zac, Zirahuén, Mich., Tulancingo, Hgo. and Amecameca, Mex. Flies were captured in nature and carried to the laboratory were individual cultures of each female were established, when the offsprig emerged salivary glands of a single larva from each culture were extracted and stained with an aceto-orcein solution and the corresponding genotype for the third and sex chromosome determined and their relative frequencies calculated. The corresponding frequencies of the “sex-ratio” inversion in the localities analyzed were: Nevado de Colima 21.8 %, Valparaiso 18.8 %, Zirahuén 11.4 %, Tulancingo 6.8 % and Amecameca 6.8 %. Apparently an East-West cline distribution is present in these populations. Relative frequencies for inversions in the third chomosome were recorded and relationships between third and X chromosomes inversions performed. Comparisons with similar studies on this and other species are pointed out.
Key words: D.pseudoobscura, natural population, sex ratio
Corresponding author: Víctor M. Salceda, Departamento de Biología, Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca S/N,La Marquesa, Ocoyoacac,México C.P. 52750, victor.salceda@inin.gob.mx
Published in „GENETIKA“ Vol. 43, No. 2 (2011), pp. 331 -340
© 2011 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.11
Original scientific paper
EVALUATION OF PHENOTYPIC DIVERSITY OF APPLE (Malus sp.) GERMPLASM THROUGH THE PRINCIPLE COMPONENT ANALYSIS
Evica MRATINIĆ,and Milica FOTIRIĆ AKŠIĆ
Faculty of Agriculture, Belgrade-Zemun, Serbia
Abstract
Mratinić E. and M. Fotrić Akšić (2011): Evaluation of phenotypic diversity of apple (Malus sp.) germplasm through the principle component analysis. - Genetika, Vol 43, No. 2, 331 - 340.
A wide range of apple cultivars representing Malus sp. germplasm were collected from southern part of Serbia, where this specie has been in cultivation for a long time. Data for twelve traits were collected for 18 cultivars for three consecutive years. Cultivars differed in phenology, fruit weight, yield and chemical composition were evaluated. Correlations were identified among phenological and compositional traits. Principal component analysis (PCA) was performed to study correlation among variables in set of apple cultivars evaluated and to interpret relationships among genotypes as a tool for germplasm characterization. Dulabija, intended for table consumption, and Stambolka, suitable for the processing, were identified as promising. Because of the large diversity identified, data should be collected for additional local apple cultivars.
Key words: autochthonous cultivars, genetic resources, phenotypic traits
Corresponding author: Milica Fotirić Akšić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: ++ 381 64 2612710, fax: ++ 381 11 2199 805, e-mail: fotiric@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 43, No.2 (2011), pp. 341 - 348
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
INHERITANCE OF EAR YIELD AND ITS COMPONENTS IN SWEET CORN
(Zea mays L. saccharat)
Jelena Srdić, Zorica Pajić, Milomir Filipović, Mliosav Babić,
and Mile sečanski
Maize Research Institute, Zemun Polje, Belgrade, Serbia
Abstract
Srdić J., Z. Pajić, M. Filipović, M.Babić and M. Sečanski (2011): Inheritance of ear yield and its components in sweet corn (Zea mays L. saccharat). - Genetika, Vol 43, No. 2, 341-348.
Sweet corn is mutation of field corn in which greater accumulation of sugars and water soluble polysaccharides provide specific taste and texture. It is consumed in the milky stage of the endosperm, so the product’s quality and its appearance are important as well as ear yield. That is why breeders pay the same attention to these traits in breeding process.
In this paper we analyzed combining ability and mode of inheritance of ear yield and yield components in sweet corn, such as kernel-row number, and ear length, by the use of diallel crosses of six sweet corn inbred lines. ANOVA showed that estimates of general (GCA) and specific (SCA) combining abilities were highly significant (p<0.01) for all observed traits. Nonaditive gene effects were more significant in the expression of ear yield and ear length, while additive gene effects were more important for kernel-row number. Analysis of components of genetic variance was in agreement with the results of ANOVA of combining ability. The mode of inheritance of kernel-row number was partial dominance, while overdominance was of greater importance for ear yield, and ear length.
Inbred line L4 was indicted as the most desirable, among the examined set of inbreds, in the further sweet corn breeding programs, due to its significant GCA effects concerning ear yield and number of kernels per ear, and ear yield performances of the hybrids in which one of the components was this inbred, according to SCA and hetrosis estimates.
Key words: diallel analysis, GCA, SCA, sweet corn
Corresponding author: Jelena Srdić, Maize Research Institute, Slobodana Bajića 1, 11185 Zemun-Belgrade, Serbia, Phone: 011/3756-704, e-mail: jsrdic@mrizp.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 349 - 359
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
CHARACTERIZATION OF VEGETATIVE AND GRAIN FILLING PERIODS OF WINTER WHEAT BY STEPWISE REGRESSION PROCEDURE.
I. VEGETATIVE PERIOD
Novo PRŽULJ, and Vojislava MOMČILOVIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Pržulj N., and V. Momčilović (2011): Characterization of vegetative and grain filling periods of winter wheat by stepwise regression procedure.I. Vegetative period. - Genetika, Vol 43, No. 2, 349- 359.
Modeling plant growth by mathematical functions is important for understanding plant development and growth. Most of the models of dry matter accumulation in small cereals simulated the period of grain filling while small attention has been devoted to mathematical simulation of vegetative period till anthesis. The aim of this research was to determine the most appropriate polynomial non-linear regression for dry matter accumulation till anthesis in winter wheat. Pobeda, a medium early variety, was used as model genotype for this research. A 5-year field data were analyzed by the forward procedure of stepwise regression. Although the procedure requires the maximum power of the polynomial regression to be used, we suggest using a lower power since it is easier for understanding and explanation and it is taking into account literature sources and biological laws. It can be accepted that quadratic regression model appropriately fits the process of dry matter accumulation till anthesis in winter wheat.
Key words: dry matter accumulation, model, stepwise regression, vegetative period
Corresponding author: Novo Pržulj, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, e-mail: novo.przulj@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 361 - 370
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
HLA CLASS II ANTIGENS AND HAPLOTYPES ASSOCIATED WITH SUSCEPTIBILITY OF LEUKEMIAS AND MYELODYSPLASTIC SYNDROME
Svetlana VOJVODIĆ and Dušica ADEMOVIĆ-SAZDANIĆ
Institute for Blood Transfusion of Vojvodina, Department for laboratory testing,
Tissue Typing Compartment, Novi Sad, Serbia
Abstract
Vojvodić S., and D. Ademović-Sazdanić (2011): HLA class II antigens and haplotypes associated with susceptibility of leukemias and myelodysplastic syndrome. - Genetika, Vol 43, No. 2, 361- 370.
Genetical and environmental factors play an interactive role in the development of acute and chronic leukemias.HLA antigens have been considered as possible genetic risk factors.The aim of this work was to investigate a possible association between HLA class II polymorphisms and leukemias and myelodysplastic syndrome.In the present study we investigated HLA class II antigens,DR/DQ and DR51/DR52/DR53 haplotypes in 100 patients:7 suffering from myelodysplastic syndrome (MDS),37 from acute lymphoblastic leukemia(ALL),32 from acute myeloid leukemia (AML) and 24 from chronic myeloid leukemia(CML).A panel of 210 healthy unrelated individuals of the same origin, from Vojvodina, served as controls.HLA phenotyping was performed by two color fluorescence method. In patients suffering from MDS was found a positive association with DR7(RR=2.598,EF=0.175) and DQ7(3)(RR=4.419, EF=0.632), while negative association was found for DR15(2)(RR=0.405, PF=0.172) and DQ6(1) (RR=0.889, PF=0,936).Positive association was found in the group of patients with ALL for DR7(RR=2.391,EF=0.688) and DQ2(RR=1.62, EF=0.15),while negative association was found with DQ5(1)(RR=0.075, PF=0.324).In the group of patients with AML,there were positive associations with DR11(5)(RR=1.732,EF=0.211),DQ2(RR= 1.594, EF=0.151) and DQ7(3) (RR=2.547,EF=0.266),while possible protective antigen was DQ5(1) (RR=0.107,RF=0.701).Higher RR than 1 and EF>0.15, in patients suffering from CML was found for DQ6(1)(RR=1.661,EF=0.232), while negative association was found for DR4 (RR=0.182,PF=0.155).Possible protective haplotype in this study was DR3DQ8(3) for patients suffering from AML(RR=0.007, PF=0.501).The distribution of DR53-DR53 haplotypes showed significant difference in male patients with ALL(6% vs 0.09%), while DR52-DR52 haplotype was significantly less frequent in male patients with CML (4% vs 20.47%) and female patients with MDS (1% vs 18.57%), respectively, in comparison to controls. We deduced that DR7 antigen in male patients with ALL has the greatest impact to the higher frequency of DR53-DR53 haplotype in this type of leukemia.The role of HLA antigens as risk factors for development of leukemias in our population was shown and furthermore it could be useful in clinical practice.
Key words: association, Human Leukocyte Antigens; leukemia
Corresponding author: Svetlana Vojvodić, Institute for Blood Transfusion of Vojvodina, Hajduk Veljkova 9a, 21000 Novi Sad, Tel.: +381 21 4877 963; fax: +381 21 4877 978,
e-mail: ssvu@EUnet.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 371-380
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
FV LEIDEN, FII G20210A AND MTHFR C677T MUTATIONS IN PATIENTS WITH LOWER OR UPPER LIMB DEEP VEIN THROMBOSIS
Valentina DJORDJEVIC1, Iva PRUNER1, Ljiljana RAKICEVIC1, Mirjana KOVAC2,3, Danijela MIKOVIC2, Predrag MILJIC2,4, Nebojsa ANTONIJEVIC2,5, and Dragica RADOJKOVIC1
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
2School of Medicine, University of Belgrade, Serbia
3Blood Transfusion Institute of Serbia, Haemostasis Department, Belgrade, Serbia
4Clinic of Hematology, Clinical Center of Serbia, Belgrade, Serbia
5Clinic for Cardiology, Clinical Centre of Serbia, Belgrade, Serbia
Abstract
Djordjevic V., I. Pruner, Lj. Rakicevic, M. Kovac, D.Mikovic, P. Miljic, N.Antonijevic, and D. Radojkovic (2011): FV leiden, FII G20210A and MTHFR C677T mutations in patients with lower or upper limb deep vein thrombosis - Genetika, Vol 43, No. 2, 371 -380.
Deep vein thrombosis (DVT) is a multifactorial disease that occurs with frequency of 1/1000 per year. The FV Leiden, FII G20210A and MTHFR C677T mutations represent genetic factors for the occurrence of vein thrombosis. The goal of this study was to determine the frequency of these mutations in patients with DVT of upper and lower limbs. The study encompassed 119 patients divided in two groups. The group of patients with the lower limbs thrombosis included 77 patients, while the upper limbs thrombosis group included 42 patients. The presence of FV Leiden, FII G20210A and MTHFR C677T mutations was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. In patients with DVT of lower limbs, the frequency of FV Leiden mutation was 26,0% in heterozygous form and 1,3% in homozygous form. In the group of patients with DVT of upper limbs, the frequency of heterozygous carriers was 7.2%. In patients with DVT of lower limbs, FII G20210A mutation occurred in heterozygous form in 15.6% subjects, and in the group with DVT of upper limbs the frequency was 7.2% in heterozygous and 2.3% in homozygous form. The frequency of MTHFR C677T mutation in patients with lower limbs DVT was 42.8% in heterozygous form and 13% in homozygous form, while in the group of patients with upper limbs DVT, the frequency was 52.4% in heterozygous form and 9.5% in homozygous form.The FV Leiden and FII G20210A mutations represent significant risk factors for the occurrence of DVT of lower limbs. These mutations are less frequent in DVT of upper limbs and more extensive further studies are needed to determine their potential role. The MTHFR C677T mutation represents less significant risk factor for lower limb DVT and should be taken into account only in cases when it occurs in combination with other risk factors.
Key words: FV Leiden, FII G20210A, MTHFR C677T, risk factors, vein thrombosis, upper and lower limb
Corresponding author: Iva Pruner,Laboratory for Molecular Biology,Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a,P.O. Box 23,11010 Belgrade,Serbia,phone: + 381 11 3976658,fax: + 381 11 3975808, e-mail: iva.pruner@gmail.com
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 555-563
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
SMALL GRAIN CEREALS COMPARED FOR DIETARY FIBRE AND PROTEIN CONTENTS
Slađana ŽILIĆ1, Dejan DODIG1a, Marija MILAŠINOVIĆ ŠEREMEŠIĆ1,Vesna KANDIĆ1, Marija KOSTADINOVIĆ1, Slaven PRODANOVIĆ2 and Đorđe SAVIĆ3
1Maize Research Institute, “Zemun Polje”, Belgrade-Zemun, Serbia
2Faculty of Agriculture, University of Belgrade, Belgrade-Zemun, Serbia
3Faculty of Medicine, University of Belgrade, Belgrade, Serbia
Abstract
Žilić S., D. Dodig, M.Milašinović Šeremešić, V.Kandić, M.Kostadinović, S. Prodanović and Đ. Savić (2011): Small grain cereals compared for dietary fibre and protein contents. - Genetika, Vol 43, No. 2, 381 - 395.
The content of dietary fibres (cellulose, hemicellulose, lignin, NDF, ADF), tryptophan and proteins, as well as their quality index were determined in whole grains of bread and durum wheat, rye, hull-less barley and hull-less oat, each represented with four genotypes. In addition, content of β-glucans in hull-less barley were determined.
In average, hull-less barley and oat had the lowest content of hemicellulose (22.54 and 13.11% d.m., respectively), cellulose (1.36 and 1.41% d.m., respectively), lignin (0.98 and 0.49% d.m., respectively), as well as NDF (24.84 and 15.16% d.m., respectively) and ADF (2.30 and 2.04% d.m., respectively). In average, the highest content of hemicellulose was in durum wheat (33.47% d.m.), followed by rye (29.63% d.m.), and bread wheat (23.24% d.m.). Among tested hull-less barley genotypes the the content of β-glucans ranged from 4.1% d.m. (IWHBON 97-18) to 5.6% d.m. (Apolon). The highest content of proteins (on average 15.65% d.m.) and tryptophan (on average 0.206% d.m.) was in hull-less oat. Hull-less barley had the highest protein quality index (1.48%) followed by bread and durum wheat and hull-less oat (IQ 1.35, 1.34 and 1.31%, respectively), and rye (IQ 0.93%). The results indicate that there is genetic diversity in content of dietary fibres and proteins among tested genotypes and that it should be possible to selectively breed for lines with high nutrition capacities, as well as, to improved diet requirements.
Key words: dietary fibre, protein, small grain cereals, sugar, tryptophan
.
Corresponding author: Slađana Žilić, Maize Research Institute, “Zemun Polje”, Department of technology, Slobodana Bajića 1, 11000 Belgrade-Zemun, Repablik of Serbia; tel.:+381113756704; fax: +381113754994; email: szilic@mrizp.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 397-406.
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.23
Original scientific paper
FLOWERING TIME AND INCOMPATIBILITY GROUPS – CULTIVAR COMBINATION IN COMMERCIAL SWEET CHERRY
(Prunus avium L.) ORCHARDS
Sanja RADIČEVIĆ, Radosav CEROVIĆ, Slađana MARIĆ, and Milena ĐORĐEVIĆ
Fruit Research Institute, Čačak, Serbia
Abstract
Radičević S., R.Cerović, S.Marić and M. Đorđević (2011): Fowering time and incompatibility groups – cultivar combination in commercial sweet cherry (Prunus avium L.) orchard - Genetika, Vol 43, No2, 397 - 406.
The paper presents results of eight-year study (1999–2006) of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time, as well as progress and abundance of flowering were studied, and classification of the studied cultivars according to flowering time was derived. On the basis of mean several-year overlap in phenophase of full flowering and on the grounds of so far known data on classification of these cultivars among incompatibility groups, we have offered a recommendation for their cultivation in orchards whereby the most effective pollination and fertilization can be ensured as well as good fruit-set and satisfactory fruit yields.
Key words: cultivar, flowering, incompatibility groups, sweet cherry
Corresponding author: Sanja Radičević, Fruit Research Institute, Kralja Petra I 9, 32000 Čačak, Serbia, Phone: 032 221 413, Fax: 032 221 391,e-mail: sanjaradicevic@yahoo.com
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 407-417.
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
VARIABILITY OF NITROGEN CONTENT IN THE NEEDLES OF DOUGLAS-FIR (Pseudotsuga menziesii Mir / Franco) PROVENANCES
Vera LAVADINOVIĆ1, Vasilije ISAJEV2, Zoran MILETIĆ1, and Milun KRSTIĆ2
1Institute of
Forestry, Belgrade, Serbia
2 Faculty of Forestry, Belgrade, Serbia
Abstract
Lavadinović V., V. Isajev, Z. Miletić, and M. Krstić (2011): Variability of nitrogen content in the needles of douglas-fir (Pseudotsuga menziesii Mir / Franco) provenance. - Genetika, Vol 43, No.2, 407-417.
Nitrogen content in the needles of twenty Douglas-fir provenances, originating from different sites within the native range of the species in the USA, was studied in a Douglas-fir provenance test established at the montane beech site on acid brown soil. Based on the variability of nitrogen content in the needles, the intensity and dynamics of the physiological processes of Douglas-fir mineral nutrition were analyzed as the indicators of Douglas-fir adaptive potential to the sites in Serbia. All the trees of the study provenances were of the same age and grown under the same site and population conditions. The quantities of nitrogen absorbed in Douglas-fir needles were correlated with the geographical characteristics of the native sites of the observed provenances. The differences in nitrogen content in Douglas-fir needles point out the variability in the intensity of the physiological processes in the genotypes of the different provenances. Since the study Douglas-fir trees are cultivated on relatively small areas, in more or less equal general conditions, it can be concluded that the parameters of mineral nutrition depend on the genotypes constituting the gene pool of the study Douglas-fir provenances.
Key words: Douglas-fir, nitrogen,provenances, Serbia
Corresponding author: Vera Lavadinović, Institute of Forestry, Kneza Višeslava 3, Belgrade, Serbia, email:veralava@eunet.rs
Published in „GENETIKA“ Vol. 43, No. 2(2011), pp. 419-426.
©
2011 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
PHENOTYPIC VARIABILITY OF BREAD WHEAT GENOTYPES FOR NITROGEN HARVEST INDEX
Biljana GORJANOVIĆ1, Milka BRDAR-JOKANOVIĆ2,
and Marija KRALJEVIĆ-BALALIĆ1
1Faculty of Agriculture, Novi Sad, Serbia
2Institute for Vegetable Crops, Smederevska Palanka, Serbia
Abstract
Gorjanović B., M. Brdar-Jokanović, and M. Kraljević-Balalić (2011): Phenotypic variability of bread wheat genotypes for nitrogen harvest index- Genetika, Vol 43, No.2, 419 -426.
Nitrogen harvest index (grain nitrogen content over total nitrogen content ratio) is a measure of the efficiency of nitrogen translocation from the vegetative portions of the plant to the grain. It can be recommended as a selection criterion for nitrogen use efficiency improvement. The aim of this study was to investigate nitrogen harvest index in twelve bread wheat genotypes at three nitrogen levels and to classify genotypes according to their phenotypic similarity for the examined trait. The results of factorial ANOVA showed that nitrogen harvest index was influenced mostly by the year × genotype interaction, year of investigation and genotype; and to the lowest extent by the applied nitrogen rate. Increasing nitrogen doses did not lead to the increased nitrogen harvest index. The calculated nitrogen harvest index values were the highest for wheat growing season 2004/05, and the smallest for the season 2006/07. The highest nitrogen harvest indices were calculated for cultivars Pobeda at the N0 rate and Zlatka at the N100 rate (0.93), and the lowest for cultivar Tamaro at the N0 rate (0.63). Analyzing the constructed dendogram, cultivars Pobeda and Renan at all three levels of nitrogen supply can be singled out as the genotypes with the highest, and cultivar Tamaro as the variety with the lowest harvest index value. The results of this study may be used in developing new high-yielding bread wheat cultivars with improved nitrogen use efficiency. Growing such cultivars would provide the savings in mineral fertilizers and minimize their possible harmful effect on environment.