GENETIKA, Vol. 44, No. 3 (2012)
Slaviša STOJKOVIĆ, Nebojša DELETIĆ,
Miroljub AKSIĆ, Dragoljub BEKOVIĆ, Vladan DJURIĆ
VARIABILITY PARAMETERS FOR YIELD COMPONENTS OF
DIFFERENT PROGENY TYPES IN AN F3 MAIZE POPULATION [Abstract] [Full
text]
Dejan CvikiĆ, Jasmina ZDRAVKOVIĆ, Nenad PavloviĆ, Slađan ADŽIĆ and Mladen ĐORĐEVIĆ
POSTHARVEST SHELF LIFE OF TOMATO (Lycopersicon esculentum Mill.) MUTANATS (nor and rin) AND THEIR HYBRIDS [Abstract] [Full text]
Naser SABAGHNIA, Rahmatollah KARIMIZADEH, Mohtasham MOHAMMADI
GROUPING GENOTYPES AND TEST ENVIRONMENTS BY SOME CLUSTER METHODS REGARDING GENOTYPE × ENVIRONMENT INTERACTION IN MULTI-ENVIRONMENT TRIALS [Abstract] [Full text]
Milomirka MADIĆ, Desimir KNEŽEVIĆ, Aleksandar PAUNOVIĆ,Dragan ĐUROVIĆ
GENETIC ANALYSIS OF SPIKE TRAITS IN TWO- AND
MULTI-ROWED BARLEY CROSSES [Abstract]
[Full
text]
Rade MILETIĆ and Svetlana M. PAUNOVIĆ
RESEARCH INTO SERVICE TREE (Sorbus domestica L.) POPULATION IN EASTERN SERBIA [Abstract] [Full text]
Victor M. SALCEDA and Carolina ARCEO MALDONADO
SEX RATIOS IN NATURAL POPULATIONS OF Drosophila pseudoobscura FROM MEXICO [Abstract] [Full text]
Novo PRŽULJ and Vojislava MOMČILOVIĆ
SPRING
BARLEY PERFORMANCES IN THE PANNONIAN ZONE [Abstract] [Full
text]
Bojana OREŠČANIN, Dragana MILADINOVIĆ, Ana
MARJANOVIĆ-JEROMELA, SlobodankaPAJEVIĆ, Milan BORIŠEV, Nataša NIKOLIĆ, Igor BALALIĆ
REACTION OF WINTER OILSEED RAPE VARIETIES TO
ELEVATED CONCENTRATIONS OF LEAD [Abstract] [Full text]
Vesna HADŽIAVDIĆ, Damir MARJANOVIĆ, Naris POJSKIĆ, Rifat HADŽISELIMOVIĆ, Kasim BAJROVIĆ, Zana DOLIĆANIN, Izet EMINOVIĆ
DIVERSITY OF NUCLEAR SHORT TANDEM REPEAT LOCI IN REPRESENTATIVE SAMPLE OF NORTH-EASTERN BOSNIAN AND HERZEGOVINA POPULATION [Abstract] [Full text]
Mirjana ŠIJAČIĆ-NIKOLIĆ, Dragica STANKOVIĆ, Borivoj KRSTIĆ, Dragica VILOTIĆ, Vladan IVETIĆ
THE POTENTIAL OF DIFFERENT LIME TREE (Tilia spp) GENOTYPES FOR PHYTOEXTRACTION OF HEAVY METALS [Abstract] [Full text]
Dubravka SAVIĆ
LEEK HYBRID YIELD POTENTIONAL AND RELATION ON ADAPTATION
TO LIGHT INTERCEPTION UNDER NITROGEN SUPPLY [Abstract] [Full text]
Suzana KRATOVALIEVA, Marija SRBINOSKA,
Gordana POPSIMONOVA, Ana SELAMOVSKA, Vladimir MEGLIC, Violeta ANDJELKOVIC
ULTRASOUND INFLUENCE ON COLEOPTILE LENGTH AT POACEAE
SEEDLINGS AS VALUABLE CRITERIA IN PREBREEDING AND BREEDING PROCESSES [Abstract] [Full text]
Milan STEVANOVIC, Snežana. MLADENOVIC DRINIC, Vesna DRAGICEVIC, Zoran CAMDŽIJA, Milomir FILIPOVIC, Nevena VELICKOVIC, Goran STANKOVIC
AN ASSESSMENT OF NUTRITIONAL QUALITY OF HYBRYD MAIZE GRAIN BASED ON CHEMICAL COMPOSITION [Abstract] [Full text]
Ivana GLIŠIĆ, Radosav CEROVIĆ, Nebojša MILOŠEVIĆ, Milena ĐORĐEVIĆ and Sanja RADIČEVIĆ
Initial and final fruit set in some plum (Prunus domestica L.) hybrids under different pollination types [Abstract] [Full text]
Primož TITAN, Vladimir MEGLIČ, and Jernej ISKRA
COMBINING ABILITY AND HETEROSIS EFFECT IN HEXAPLOID WHEAT GROUP [Abstract] [Full text]
Suzana PAVLOVIĆ, Slađan ADŽIĆ, Dejan CVIKIĆ, Jasmina
ZDRAVKOVIĆ, Milan Zdravković
IN VITRO
CULTURE AS A PART OF BRASSICA OLERACEA VAR. CAPITATA L. BREEDING [Abstract] [Full
text]
Slavica Čolić, Vera Rakonja, Milica Fotirić Akšić, Dragan Nikolić, Vladislav OGNJANOVand Dragan RAHOVIĆ1
Dehydrogenase Isoenzyme Polymorphism in genus Prunus, subgenus Cerasus [Abstract] [Full text]
Biljana Kuzmanovska, Rade Rusevski, Ljupcho Jankulosk, Mirjana Jankulovska, Dario IVIC, Katerina Bandzo
PHENOTYPIC AND GENETIC CHARACTERIZATION OF BOTRYTIS CINEREA ISOLATES FROM TOMATO [Abstract] [Full text]
Milica RADOSAVLJEVIĆ, Marija MILAŠINOVIĆ-ŠEREMEŠIĆ, Dušanka TERZIĆ, Goran TODOROVIĆ, Zorica PAJIĆ, Milomir FILIPOVIĆ, Željko KAITOVIĆ, Snežana MLADENOVIĆ DRINIĆ
EffectS of hybrid on maize grain and plant carbohydrates [Abstract] [Full text]
Maja TOLINAČKI, Jelena LOZO, Katarina VELJOVIĆ, Milan KOJIĆ, Đorđe FIRA and Ljubiša TOPISIROVIĆ
EXAMINATION OF ANTIMICROBIAL POTENTIAL IN NATURAL ISOLATES OF LACTOBACILLUS CASEI/PARACASEI GROUP [Abstract] [Full text]
Mohammad JOUKI , Ahmad DADASHPOUR
COMPARISON OF PHYSIOCHEMICAL CHANGES IN TWO POPULAR STRAWBERRY CULTIVARS GROWN IN IRAN (cvs. KURDISTAN & SELVA) DURING STORAGE TIME AT 4 °C [Abstract] [Full text]
Aleksandar Lučić, Vasilije Isajev, Ljubinko Rakonjac, Milan Mataruga, Vladan Popović, Radovan Nevenić and Snežana Mladenović Drinić
ANALYSIS OF INTER – POPULATION VARIABILITY OF SCOTS PINE (Pinus sylvestris L.) USING MORPHOMETRIC MARKERS [Abstract] [Full text]
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Milan ZDRAVKOVIĆ, Mirjana MIJATOVIĆ, Nenad PAVLOVIĆ
THE USAGE OF MUTANT GENOTYPES IN TOMATO SELECTION FOR SPECIFIC TRAITS AT THE INSTITUTE FOR VEGETABLE CROPS [Abstract] [Full text]
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 441-448
© 2012 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
DOI: 10.2298/GENSR1203441S
Original scientific paper
VARIABILITY PARAMETERS FOR YIELD COMPONENTS OF DIFFERENT PROGENY TYPES IN AN F3 MAIZE POPULATION
Abstract
Stojakovic S., N. Deletic, M. Aksic, D. Bekovic and V. Djuric (2012): Variability parameters for yield components of different progeny types in an F3 maize population. Genetika, Vol 44, No. 3, 441-448.
This paper presents the investigation of F3 population 2002-30, created in the Department of Maize, Institute of Field and Vegetable Crops, Novi Sad. At first, about 150 plants of the studied population were selfed and simultaneously crossed with the tester 1491x1496, in order to get complementary S1 and HS progenies. After the harvest, 41 progenies of each type were selected by the random sample method for the trials carried out in Aleksinac, Leskovac, and Kruševac, as three studied environments, in RCB design. Genetic variances were significantly higher in S1 progenies, with the exception of root and stalk lodged plants percent and grain number per row, where these differences were not significant. Phenotypic variances followed the same tendency. The obtained values of broad-sense heritability were significantly higher in S1 than in HS progenies. Low heritability values were calculated only for root and stalk lodged plants percent. Cluster analysis did not show any greater difference between S1 and HS progenies, neither in distances nor in group definition, although there were significant differences between them in genetic variances. However, one can see a lower degree of distances between HS progenies.
‘ Key words: grain yield, yield components, maize, variability
Corresponding author: Slaviša Stojković, Faculty of Agriculture Kosovska Mitrovica – Lešak, Kopaonička bb, 38219 Lešak, Serbia, Tel/fax: +3812888261, E-mail: slavisa.stojkovic@pr.ac.rs
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 449-456
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635.64
Original scientific paper
POSTHARVEST SHELF LIFE OF TOMATO (Lycopersicon esculentum Mill.) MUTANATS (nor and rin) AND THEIR HYBRIDS
Dejan CvikiĆ, Jasmina ZDRAVKOVIĆ, Nenad PavloviĆ, Slađan ADŽIĆ
and Mladen ĐORĐEVIĆ
1Institute for Vegetable Crops, Smederevska Palanka, Serbia
Abstract
Cvikic D., J.Zdravkovic, N.Pavlovic, S.Adžić and M. Đorđevic (2012): Postharvest shelf life of tomato (Lycopersicon esculentum mill.) mutanats (nor and rin) and their hybrid.. - Genetika, Vol 44, No. 3, 449-456.
Two tomato lines with normal maturation (NR-1 and NR-2) have been crossed with two mutant lines (NR-10 nor and NR-12 rin) with delayed maturation (shelf life). Determination of mutant genes has been done by χ2 test on 100 fruits from F2 generation. Fruits have been picked 65 days from antesis and kept for 60 days, when six evaluations have been done. Data have been collected every 10 days on parental lines and progeny F1 and F2 generation. Variance testing has been done on the basis of one- and two-factorial analysis and groups compared by contrasts. Fruits have been preserved in controlled conditions (in dark at 5oC). Tomato genotypes with nor or rin gene had desirable traits (delayed ripening, long shelf life and firm fruits) for modern selection, so they should be included in programmes aiming to create commercial F1 hybrids.
Key words: hybrid, mutant, nor, rin, tomato
Corresponding author: Dejan Cvikić, Institute for Vegetable Crops, Karađorđeva 71, 11420 Smederevska Palanka, Serbia, Phone: 381 26 323 170, fax 381 26 323 785, e-mail: dcvikic@institut-palanka.co.rs
Published in „GENETIKA“ Vol. 44, No. 3 (2012), pp. 457 -473
© 2012 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635.62
Original scientific paper
GROUPING GENOTYPES AND TEST ENVIRONMENTS BY SOME CLUSTER METHODS REGARDING GENOTYPE × ENVIRONMENT INTERACTION IN MULTI-ENVIRONMENT TRIALS
Naser SABAGHNIA1*, Rahmatollah KARIMIZADEH2, Mohtasham MOHAMMADI2
Department of Agronomy and Plant Breeding, Faculty of Agriculture,
University of Maragheh, Maragheh, Iran.
2Dryland Agricultural Research Institute (DARI), Gachsaran, Iran
Abstract
Sabaghnia N., R. Karimizadeh, and M. Mohammadi (2012): Grouping genotypes and test environments by some cluster methods regarding genotype × environment interaction in multi-environment trials. - Genetika, Vol 44, No. 3, 457 - 473.
Lentil (Lens culinaris Medik.) is an important source of protein and carbohydrate food for people of developing countries and is popular in some developed countries where they are perceived as a healthy component of the diet. Ten lentil genotypes were tested for grain yield in five different environmental conditions, over two consecutive years to classify these genotypes for yield stability. Seed yield of lentil genotypes ranged from 989.3 to 1.367 kg ha-1 and the linear regression coefficient ranged from 0.75 to 1.18. The combined analysis of variance showed that the effect of environment (E) and genotype by environment (GE) interaction were highly significant while the main effect of genotype (G) was significant at 0.05 probability level. Four different cluster procedures were used for grouping genotypes and environments. According to dendograms of regression methods for lentil genotypes there were two different genotypic groups based on G plus GE or GE sources. Also, the dendograms of ANOVA methods indicated 5 groups based on G and GE sources and 4 groups based on GE sources. According to dendograms of regression methods for environments there were 5 different groups based on G plus GE sources while the dendograms of ANOVA methods indicated 9 groups based on G and GE sources and 3 groups based on GE sources. The mentioned groups were determined via F-test as an empirical stopping criterion for clustering. The most responsive genotypes with high mean yield genotypes are G2 (1145.3 kg ha-1), G8 (1200.2 kg ha-1) and G9 (1267.9 kg ha-1) and could be recommended as the most favorable genotypes for farmers.
Key words: GE interaction, dendogram, grouping methods, seed yield
Corresponding author: Naser Sabaghnia, Department of Agronomy and Plant Breeding, Faculty of Agriculture,University of Maragheh,Maragheh, P.O. Box 55181-83111, Iran,Tel: +98-421-2273070,Fax: +98-421-2276060, E-mail sabaghnia@maragheh.ac.ir, sabaghnia@yahoo.com
Published in „GENETIKA“ Vol. 44, No.3 (2012), pp. 475 -.482
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575: 633. 16
Original scientific paper
GENETIC ANALYSIS OF SPIKE TRAITS IN TWO- AND MULTI-ROWED BARLEY CROSSES
Milomirka MADIĆ1, Desimir KNEŽEVIĆ2, Aleksandar PAUNOVIĆ1,
Dragan ĐUROVIĆ1
1University of Kragujevac, Faculty of Agronomy, Čačak, Serbia
2University of Priština, Faculty of Agriculture, Lešak, Serbia
Abstract
Madić M., D. Knežević, A. Paunović and D. Đurović (2012): Genetic analysis of spike traits in two- and multi-rowed barley crosses - Genetika, Vol. 44, No3, 475-482.
Mode of inheritance and genetic variance components for spike length, grain number per spike and grain weight per spike were evaluated in four parental genotypes and their F1 and F2 hybrids obtained through incomplete diallel crossing. Multi-rowed barley genotypes HVW-247 and Partizan and two-rowed barley genotypes KG-15 and NS-293 were selected for the crossing based on the trait concept with the parents being divergent for spike length, grain number per spike and grain weight per spike.
The average values for spike length in F1 and F2 generations were intermediate or close to those of the parent having longer spikes, with the mode of inheritance being partial dominance, dominance or overdominance, depending on the crossing combination. The six-rowed x six-rowed and two-rowed x two-rowed crossing combinations showed dominance of increased grain number per spike and increased grain weight per spike in the inheritance of grain number per spike and grain weight per spike, respectively, whereas the six-rowed x two-rowed cross was predominated by partial dominance.
Key words: barley, diallel analysis, gene effect, spike traits
Corresponding author: Milomirka Madić, University of Kragujevac, Faculty of Agronomy, Cara Dušana 34, 3200 Čačak Phone +38132 303 410;
e-mail: mmadic@tfc.kg.ac.rs
Published in „GENETIKA“ Vol. 44, No.3 (2012), pp. 483-490
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
RESEARCH INTO SERVICE TREE (Sorbus domestica L.) POPULATION
IN EASTERN SERBIA
Rade MILETIĆ and Svetlana M. PAUNOVIĆ
Fruit Research Institute Čačak, Serbia
Abstract
Miletic R., and S. M.Paunovic (2012): Research into service tree (Sorbus domestica L.) population in Eastern Serbia - Genetika, Vol 44, No. 3, 483-490.
All fruit tree populations along with the service tree (Sorbus domestica L) population offer a wealth of diverse genetic material which is essential for the selection and creation of new cultivars and rootstocks. The main objective of the study was to evaluate the service tree population in Eastern Serbia in order to single out good selections whose fruit can be used fresh or processed in the human diet, as well as genotypes suitable for the development of new cultivars and rootstocks. The service tree population was analyzed for tree age, tree size and major fruit properties (shape, size and soluble solids content). Small-sized, medium to large and large fruits ranging in weight from 7.5-9.8 g, 10.3-18.6 g and 21.7-25.6 g were found in 52.3%, 41.1% and 6.60% trees, respectively, within the test population. Fruit length was 18.6-33.4 mm, fruit thickness 22.8-37.4 mm, stalk length 1.8-3.7 mm, soluble solids content 15.7-22.5%. The study also presents properties of some superior trees which require greater attention in terms of propagation, collection activities and commercial cultivation.
Key words: fruit property, population, service tree
Corresponding author: Rade Miletić, Fruit Research Institute,Kralja Petra I, 9,32000 Čačak, Serbia,Tlf. 032/221-375,Fax 032/221-391,e-mail; radem@tfc.kg.ac.rs
Published in „GENETIKA“ Vol. 44, No. 3 (2012), pp. 491-498
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
SEX RATIOS IN NATURAL POPULATIONS OF Drosophila pseudoobscura FROM MEXICO
Victor M. SALCEDA and Carolina ARCEO MALDONADO
Departamento de Biología, Instituto Nacional de Investigaciones Nucleares,
Carretera México-Toluca S/N, La Marquesa, Ocoyoacac, Edo. de México
Abstract
Salceda V.M., and C. Arceo Maldonado (2012): Sex ratios in natural populations of Drosophila pseudoobscura from Mexico - Genetika, Vol 44, No. 3, 491 - 498.
Most species show an equal proportion of individuals of both sexes. In diploid species sex ratio is determined by a genic balance between sex chromosomes. In Drosophila sex is determined by the ratio of X-chromosomes versus autosomes and in some species of the genus it is related to the presence of an inversion in the sex chromosome. The present work analyses the sex ratio in 27 natural populations of Drosophila pseudoobscura that inhabit Mexico. Female flies captured in nature were counted and their sex ratio calculated and been called generation P, then cultured individualy, allowed to leave adult offspring which was quantified in order to get its sex ratio and designated generation F1. sex ratio was calculated using the expression: number of males times 100 divided by the number of females proposed by Darwin (1871). The sex ratio of each population was taken using the average of all the individual counts from each sample. The values found varied among different generations and populations, so for generation P their values varieded 37.4 to 190.4 and in generation F1 from 31.3 up to 96.4 males for each 100 females. According to their geographical distribution four North to South transects were arranged and in them means varied from 60.8 to 81.7 males for each 100 females. All this means that in Mexican population are more females than males, exceptionally more males than females.
Key words: Drosophila pseudoobscura, population, sex
Corresponding author: Victor M. Salceda, Departamento de Biología, Instituto Nacional de Investigaciones Nucleares, km 36.5 Carretera México-Toluca S/N,La Marquesa, Ocoyoacac, Edo. de México .P. 52750, victor.salceda@inin.gob.mx.
Published in „GENETIKA“ Vol. 43, No. 3(2012), pp. 499-512
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.16
Original scientific paper
SPRING BARLEY PERFORMANCES IN THE PANNONIAN ZONE
Novo PRŽULJ1 and Vojislava MOMČILOVIĆ1
1Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Pržulj N., and V. Mihajlović (2012): Spring barley performances in the Pannonian zone. - Genetika, Vol 44, No. 3, 499 - 512.
Environmental conditions in the Pannonian zone can be characterized with moderate high temperature and partially water deficit during grain filling of spring barley, although low temperature and water deficit are possible also in period till anthesis. This study was conducted to evaluate the variation of the duration of the period from emergence to anthesis (VP), duration of grain filling period (GFP), plant height (PH), spikes number m-2 (SN), grains number spike-1 (GN), thousand grains weight (GW) and yield (YIL) in spring two-rowed barley in conditions of the Pannonian zone. All three factors; genotype, environment and the interaction GxY affected the studied traits. Average VP was 777 GDD, GFP 782 GDD, PH 78 cm, SN 523, GN 28.2, GW 43.2 g and YIL 6.26 t ha-1. Variation across varieties was higher than across growing seasons. Heritability varied from 0.66 for YIL to 0.94 for VP and GFP. This study confirmed that a sufficiently large genetic variability must be base for selecting appropriate varieties for the Pannonian zone conditions. In order to determine high yielding and quality barley extensive research in relation to breeding, variety choice for production and growing practice must be done.
Key words: Barley (Hordeum vulgare L.), Pannonian zone, variability, yield, yield components
Corresponding author: Novo Pržulj, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, e-mail: novo.przulj@ifvcns.ns.ac.rs, Telefon: 021 4898 220, fax: 021 4898 222
Published in „GENETIKA“ Vol. 43, No.3(2011), pp. 513 -519
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.85
Original scientific paper
REACTION OF WINTER OILSEED RAPE VARIETIES TO ELEVATED CONCENTRATIONS OF LEAD
Bojana OREŠČANIN1, Dragana MILADINOVIĆ2, Ana MARJANOVIĆ-JEROMELA2, Slobodanka PAJEVIĆ1, Milan BORIŠEV1, Nataša NIKOLIĆ1,
Igor BALALIĆ2
1University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad
2Institute of Field and Vegetable Crops, Novi Sad
Abstract
Oreščanin B., D. Miladinović, A. Marjanović-Jeromela, S. Pajević, M. Borišev, N. Nikolić and I. Balalić (2012): Reaction of winter oilseed rape varieties to elevated concentrations of lead. - Genetika, Vol 44, No. 3, 513-519.
Remediation methods allow the removal of metals from contaminated soil, and phytoremediation a technology for cleaning contaminated soil and waste material by plants, is becoming increasingly used. Brassica napus L., as one of the main oilcrops and high-biomass producing species, is becoming more and more interesting for the use in phytoextraction as it is proved to be tolerant to higher concentrations of heavy metals. The aim of this study was to examine the specific responses of three commercial winter rapeseed varieties, Banaćanka, Slavica and Kata, to the increased concentrations of lead in vitro. Significant reduction in root length of plants treated with lead was observed only in the variety Slavica, indicating susceptibility of this variety to the increased concentrations of this heavy metal. As in variety Kata a significant reduction in the length of the above-ground part due to the treatment with lead was detected, it could be concluded that the variety Banaćanka is the most tolerant to the applied concentrations of lead since there were no significant changes in the growth and biomass accumulation in all treatments except one, and could be recommended for further use in phytoremediation studies.
Key words: Brassica napus L., lead, tolerance, phytoremediation
Corresponding author: Ana Marjanović-Jeromela, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad; Phone: 0214898115; Fax: 021 6413833; E-mail: ana.jeromela@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 44, No.3 (2012), pp. 521-536
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
DIVERSITY OF NUCLEAR SHORT TANDEM REPEAT LOCI IN REPRESENTATIVE SAMPLE OF NORTH-EASTERN BOSNIAN AND HERZEGOVINA POPULATION
Vesna HADŽIAVDIĆ1, Damir MARJANOVIĆ 3,4, Naris POJSKIĆ3, Rifat HADŽISELIMOVIĆ2,3, Kasim BAJROVIĆ3, Zana DOLIĆANIN5, Izet EMINOVIĆ2
1 Faculty of Science, Department of Biology, University of Tuzla, Tuzla, B&H
2 Faculty of Science, Department of Biology, University of Sarajevo, Sarajevo, B&H
3 Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
4 Genos. d.o.o., Zagreb, Croatia
5State University of Novi Pazar, Novi Pazar, Serbia
Abstract
Hadžiavdić V., D. Marjanović, N. Pojskić,R. Hadžiselimović, K. Bajrović, Z. Dolićanin, and I.Eminović (2012): Diversity of nuclear short tandem repeat loci in representative sample of North-Eastern Bosnian and Herzegovina population. - Genetika, Vol 44, No. 3,521 -536.
Diversity of nuclear microsatellite markers were analyzed in a reference sample of the population of northeast Bosnia and Herzegovina. 437 samples taken from unrelated individuals were processed and three samples of paternity proof were shown. Detection effectiveness profile of the research, points to a valid choice of method of extraction, amplification and genotyping short tandem repeat (STR) loci with PowerPlextm16 kit. Genetic analysis of allelic variants of the 15 STR loci PowerPlextm16 kit detected 17 samples determined as rare allelic variants or microvariants. Samples were divided into 15 different allelic variants at 7 different loci, and are: in locus D7S820, D16S539, D3S1358, D18S51, PENTA D, PENTA E and in locus vWA. Genetic analysis of mutations in cases of paternity determined three examples of single-step mutations in the loci FGA, Penta D and D3S1358. Genetic analysis of observed STR loci detected three allelic variant of genotype combination 7/10/11.3 in locus D7S820 Type II. Population genetic analysis of STR loci in a representative sample of the population of northeast Bosnia and Herzegovina included the application of the assessment tests of within-population genetic diversity and interpopulation diversity, as well as genetic differentiation between populations: North-eastern Bosnia and Herzegovina (BH) and BH general reference, then the Croatian population, Macedonian, Serbian and Slovenian. Based on the result analysis of specific forensic parameters, it can be assumed that the most informative marker is PENTA E for population genetic analysis and forensic testing in the population of northeast Bosnia and Herzegovina. Research results fit regional STR database of this part of Europe.
Key words: genetic diversity, microvariants, mutations, North-eastern Bosnia and Herzegovina, STRs, three allelic variant
Corresponding author: Eminović Izet, Faculty of Science, Department of Biology, University of Sarajevo, Sarajevo, Bosnia and Herzegovina, Zmaja od Bosne 33-35. Tel/ +387 33 723 723. Fax/ +387 33 649 359., E-mail: eminovicizet@.gmail.com
Published in „GENETIKA“ Vol. 44, No. 3 (2012), pp. 537-548
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
THE POTENTIAL OF DIFFERENT LIME TREE (Tilia spp) GENOTYPES FOR PHYTOEXTRACTION OF HEAVY METALS
Mirjana ŠIJAČIĆ-NIKOLIĆ1, Dragica STANKOVIĆ1, Borivoj KRSTIĆ2,
Dragica VILOTIĆ1, Vladan IVETIĆ1
1Faculty of Forestry – University of Belgrade, Belgrade, Serbia
2Faculty of Natural Sciences – University of Novi Sad, Novi Sad, Serbia
Abstract
Šijačić-Nikolić M., D. Stanković, B. Krstić, D. Vilotić, and V. Ivetić (2012): The potential of different lime tree (Tilia spp) genotypes for phytoextraction of heavy metals . - Genetika, Vol 44, No. 3, 537-548.
The research of heavy metals contents (Pb, Mn, Zn, Ni, Fe) in soil in the area of the National Park „Fruška gora”, along the highway M21 shows lower values for manganese, zinc and iron than the maximum allowed quantity prescribed by law. For nickel and lead it shows higher values than maximum allowed quantity. The heavy metals contents in leaves of lime tree in 12 analyzed genotypes are far below average values in accordance with ECCE with all genotypes except genotype 7 for lead and genotypes 7 and 8 for iron. The results of analysis of variance components show that out of four components (locality, genotype, locality x genotype and error) only the interaction between locality and genotype does not contribute to variance. The contents of Pb, Mn, Fe and Zn in leaves is primarily influenced by genotype while Ni contents may be considered a consequence of locality. The selection of genotypes which is able to uptake greater quantities of heavy metals than other genotypes may serve as a solid basis for phytoextraction of heavy metals as a technology by which heavy metals, metalloids and radionuclides are extracted from environment through usage of suitable species and plant genotypes able to uptake and accumulate the given pollutants in parts of plant tissue.
Key words: genotypes, heavy metals, lime tree
Corresponding author: Mirjana Šijačić-Nikolić, Faculty of Forestry – University of Belgrade, no.1 Kneza Viseslava street, 11000 Belgrade, Serbia, tel. +381 11 3053 873, e-mail: sijacic68@open.telekom.rs
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 549-559.
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635
Original scientific paper
LEEK HYBRID YIELD POTENTIONAL AND RELATION ON ADAPTATION TO LIGHT INTERCEPTION UNDER NITROGEN SUPPLY
Dubravka SAVIĆ
University of Belgrade, Faculty of Agriculture, Institute of Field and Vegetable Crop Science, Belgrade, Serbia
Abstract
Savić D. (2012): Leek hybrid yield potentional and relation on adaptation to light interception under nitrogen supply. - Genetika, Vol 44, No. 3,549 -559.
We have studied the effect of nitrogen supply on growth as well as relation on adaptation to light interception of leek (Allium porrum L.,) hybrid Alita, genotype of known genetic background. During the vegetative and generative plant growth phases, besides genetic potential many factors affect their productivity. The aim was to investigate genome expression dependent on nitrogen nutrition and light interception. Nitrogen in correlation with light availability has important effect on the growth of plants and the formation of leaf area, what it is necessary for yield of dry matter. Investigation has been done in open field grown leek commercial hybrid Alita (Allium porrum L.,) to consider the way of its genotype response to correlation of light interception and nitrogen nutrition. Investigated traits are leek crop productivity, light interception and chemical analyses of plants. Leek crop productivity was determined through the dry matter production, leaf area development and light interception. Analyses of leek plants comprehended chemical determination and calculation of total nitrogen concentration, nitrogen critical concentration in dry matter, nitrogen demand and, nitrogen uptake in leek crop. Correlation among investigated parameters was assigned to comprehensive hypothetical model of growth and productivity of leek crop grown at open field.It was shown that for nitrogen uptake (Nu), nitrogen demand (ND) and total nitrogen concentration (Nt) parameters variants of mineral nutrition plays significant role (pNu=0.002; pND=0.045; pNt=0.011). Obtained results indicated that correlation of nitrogen and light interception could be used as criteria in plant breeding.
Key words: leek hybrid, light interception, nitrogen, dry matter, yield
Corresponding author: Dubravka,Savić, University of Belgrade, Faculty of Agriculture, Institute of Field and Vegetable Crop Science, 11080 Belgrade, Serbia
Published in „GENETIKA“ Vol. 44, No. 3 (2012), pp. 561-570
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:636
Original scientific paper
ULTRASOUND INFLUENCE ON COLEOPTILE LENGTH AT POACEAE SEEDLINGS AS VALUABLE CRITERIA IN PREBREEDING AND BREEDING PROCESSES
Suzana KRATOVALIEVA1, Marija SRBINOSKA2, Gordana POPSIMONOVA1, Ana SELAMOVSKA1, Vladimir MEGLIC3, Violeta ANDJELKOVIC4
1Institute of Agriculture, University St. Cyril and Methodius, Skopje, Republic of Macedonia
2 Scientific Tobacco Institute, University St. Kliment Ohridski, Prilep, Republic of Macedonia
4Agriculture Institute of Slovenia, Ljubljana, Slovenia
4Maize Research Institute “Zemun Polje”, Belgrade-Zemun, Serbia
Abstract
Kratovalieva S., M. Srbinoska, G. Popsimonova, A. Selamovska, V. Meglić, and V. Anđelković (2012): Ultrasound influence on coleoptile length at Poaceae seedlings as valuable criteria in prebreeding and breeding processes. - Genetika, Vol 44, No. 3, 561 -570.
The study includes research on the effect of ultrasound on the ability of seed germination and coleoptile development of cereal landraces (fam. Poaceae): oat (Avena sativa L.) brodski, rye (Secale cereale L.) rakotinska, Triticale svetinikolsko and soft wheat (Triticum aestivum L.) govrlevska. The experiment with ultrasound was carried out at frequency of 30-40 kHz on the thermostatic ultrasonic bath. Seeds were sonicated at a constant temperature (25ºC) for 15 min. Ultrasound effect is reflected in the length of the coleoptyl and mesocotyl, although in a good deal is genetically predetermined. Coleoptile length was the longest in Triticale (3.3-5.5 cm) and approximately the same lengths are evident in rye (3.0-6.1 cm). Concerning the mesocotyl the longest one (5.525 cm ± 0.697) is recorded at rye, even significantly longer than control of oat and Triticale. Longer coleoptile of sonicated seeds indicates faster seedling development, good water supply and rapid development and emergence of first leaves. Correlation coefficient showed very high (0.821) and high R2 (67,472%) dependence among variables both, in length of coleoptiles (as dependent variable) and mesocotyl (as independent) with low standard error (0.225). With the simple use of ultrasound the germination period could be shorten, water supply more efficient, the sowing periods will be shorten and good yields even under conditions of climate change with increased temperatures could be achieved
Key words: coleoptile length, mesocotyl, seedlings, ultrasound.
Corresponding author: Suzana Kratovalieva, Institute of Agriculture, Aleksandar Makedonski bb, 1000 Skopje, R.Macedonia, Phone/fax +38923230910/+38923114283,E-mail: suzanakrat@yahoo.com
Published in „GENETIKA“ Vol. 44, No.3 (2012), pp. 571 -582
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
AN ASSESSMENT OF NUTRITIONAL QUALITY OF HYBRYD MAIZE
GRAIN BASED ON CHEMICAL COMPOSITION
Milan STEVANOVIC1, Snežana. MLADENOVIC DRINIC1, Vesna
DRAGICEVIC1, Zoran CAMDŽIJA1, Milomir FILIPOVIC1, Nevena
VELICKOVIC2, Goran STANKOVIC1
1Maize Research Institute “Zemun Polje”, Belgrade, Serbia;
2Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia
Abstract
Stevanovic M., S. Mladenovic Drinic, V. Dragicevic, Z. Camdžija,M. Filipovic, N.Velickovic, and G. Stankovic (2012): An assessment ofnutritional quality of hybryd maize grain based on chemical composition. -Genetika, Vol. 44, No. 3, 571-582.
The aim of this study was to investigate chemical composition ofgrain of 20 maize hybrids, from different maturity groups, to define theirnutritional quality from the point of main grain constituents: starch, totalproteins, soluble proteins, oil, phytate, inorganic phosphorus, and soluble phenolics, as well as mass of 1.000 grains. A set of 20 ZP hybrids, FAO 400-800, were grown in a randomized complete block design (RCBD) at Zemun Polje (Serbia), during the summer of 2009. Average content of proteins, oil and starch of analyzed maize hybrids was 10.5%, 4.7% and 80.8%, respectively. Hybrids from FAO maturity group 600 had highest grain mass (in average 419.5 g). Genetic variability in seed phytate content was observed, with values ranging from 2.64 to 3.75, averaged 3.44 g kg-1. Inorganic phosphorus (Pi) concentrations were between 0.120 μg g-1 (ZP 805) to 0.415 μg g-1 (ZP 588ex), averaged 0.24 μg g-1. Content of soluble proteins varied from 54.33 to 78.74 mg g-1. The high positive correlation was observed between total proteins and oil content in hybrids, as well as
between starch and phenolics content. Phytate content was positively correlated with proteins, but this correlation was not significant, while the correlation between phytate and mass of 1,000 grains was positive and significant. A negative correlation occurred between phytate and starch.
Key words: grain content, maize, nutritional value
Corresponding author: Milan Stevanovic,Maize Research Institute “Zemun Polje”,Slobodana Bajica 1, 11185 Zemun Polje, Serbia, E-mail: mstevanovic@mrizp.rs Tel: +381 60 704 19 82
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 583-593
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
Initial and final fruit set in some plum (Prunus domestica L.) hybrids under different pollination types
Ivana GLIŠIĆ, Radosav CEROVIĆ, Nebojša MILOŠEVIĆ,
Milena ĐORĐEVIĆ and Sanja RADIČEVIĆ
Fruit Research Institute, Čačak, Serbia
Abstract
Glišić I., R. Cerović, N. Milošević, M.Đorđević, and S. Radičević (2012): Initial and final fruit set in some plum (prunus domestica L.) hybrids under different pollination types. - Genetika, Vol 44, No. 3, 583-593.
The paper presents results of two-year study (2009–2010) of initial and final fruit set in promising plum (Prunus domestica L.) hybrids developed at Fruit Research Institute – Čačak, under different pollination conditions. The following hybrids were studied: 38/62/70 (‘Hall’ x ‘California Blue’), 32/21/87 (‘Stanley’ x ‘Scoldus’), IV/63/81 (‘Large Sugar Prune’ x ‘Scoldus’), 22/17/87 (‘Čačanska Najbolja’ x ‘Zh'lta Butilcovidna’), 29/29/87 (‘Stanley’ x ‘Scoldus’) and 34/41/87 (‘Valjevka’ x ‘Čačanska Lepotica’). Each of the hybrids was studied both under self-pollination and open pollination. In vitro pollen germination was also performed as well as characteristics of flowering phenophase and flowering abundance. Generally, the results suggest lower flowering abundance in the second year of the study. Pollen germination ranged from averagely 25.31% (29/29/87) to 40.01% (38/62/70). With averagely 31.59% final fruit set under self-pollination and 29.38% under open pollination variants, respectively, hybrid 34/41/87 gave the best results. The lowest performance was observed in hybrid 32/21/87 with 1.61% and 7.69% final fruit set under self- and open pollination variants, respectively.
Key words: flowering, fruit set, plum, pollen, promising hybrids
Corresponding author: Ivana Glišić, Fruit Research Institute, Kralja Petra I 9, 32000 Čačak, Serbia, Phone: 032/221-375, Fax: 032/221-391, E-mail: glisiciva2004@yahoo.com
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 595-609
© 2012 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
COMBINING ABILITY AND HETEROSIS EFFECT
IN HEXAPLOID WHEAT GROUP
Primož TITAN1 , Vladimir MEGLIČ 2, and Jernej ISKRA3
1SEMENARNA Ljubljana, d.d., Ljubljana, Slovenia
2Agricultural Institute of Slovenia, Ljubljana, Slovenia
3 “Jožef Stefan” Institute, Ljubljana, Slovenia
Abstract
Titan P., V. Meglič, and J. Iskra (2012): Combining ability and heterosis effect in hexaploid wheat group. - Genetika, Vol 44, No. 3,595 -609.
The main goal of hybrid wheat breeding is the identification of parents with high specific combining ability for grain yield and other agronomic traits. This kind of data facilitate the development of hybrid combinations with high level of heterosis in first filial generation (F1 generation). The use of species from the hexaploid wheat group (e.g. Triticum spelta L. Triticum compactum HOST...) is representing an opportunity for the increase of heterosis level in the germplasm of common wheat (Triticum aestivum L.).
The study of combining ability and heterosis effect in hexaploid wheat group was carried out using crosses between thirteen inbred lines of common wheat (6 lines x 7 testers) and inter-species crosses (T. aestivum L. × T. spelta L., T. aestivum L. × T. compactum HOST, T. aestivum L. × T. sphaerococcum PERCIV., T. aestivum L. × T. macha DEKAPR. et MENABDE, T. aestivum L. × T. petropavlovskyi UDACZ. et MIGUSCH, T. aestivum L. × T. vavilovii (THUM.) JAKUBZ.). The 42 common wheat F1 hybrids were tested during two seasons (2010/11 and 2011/12) on the Selection center Ptuj. The experiment was carried out in a randomized block design with four replications. The 43 interspecies F1 hybrids were tested on the same location in the season 2011/12 and the experiment was designed as an randomized block with three replications. The results were analyzed using statistical package AGROBASE generation II and STATGRAPHICS Centurion XVI.
The analysis of variance was significant for both, GCA and SCA variances (P < 0,01). Generally, SCA variances were lower than GCA variances. We could state, that the improvement of heterosis level in the common wheat germplasm through the use of relatives with the same genome (genome BAD) is possible. As an example we can point out the interspecies F1 hybrid between common wheat variety Garcia and an accession of the Triticum sphaerococcum PERCIV. species (accession number 01C0201227).
Key words: combining ability, heterosis effect, hexaploid wheat group, hybrid wheat
Corresponding author: Vladimir Meglič, Agricultural Institute of Slovenia, Hacquetova ulica 17, 1000 Ljubljana, Slovenia, Phone: 00386 1 28 05 180. Fax: 00386 1 28 05 255.E-mail: vladimir.meglic@kis.si.
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 611-618
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635
Original scientific paper
IN VITRO CULTURE AS A PART OF BRASSICA OLERACEA VAR. CAPITATA L. BREEDING
Suzana PAVLOVIĆ, Slađan ADŽIĆ, Dejan CVIKIĆ, Jasmina ZDRAVKOVIĆ, Milan Zdravković
Institute for Vegetable Crops, Smederevska Palanka, Serbia
Abstract
Pavlovic S., S. Adžić, D.Cvikić, J. Zdravković and M. Zdravković (2012): In vitro culture as a part of Brassica oleracea var. capitata L. breeding. - Genetika, Vol 44, No. 3, 611 -618.
Fourteen genotypes of cabbage (Brassica oleracea var. capitata L.), that are a part of Institute for Vegetable Crops collection, were tested for their ability to regenerate shoots in vitro. Five of them are early, while nine are late genotypes. Lateral buds from plants grown in the open field were used as explants. In all genotypes, lateral buds showed the high percentage of shoot formation, ranged from 80% to 100%. They were incubated on Murashige and Skoog’s (MS) media supplemented with 1.0 and 2.0 mgl-1 of benzyladenine (BA) or 1.0 mgl-1 6-furfurylaminopurine (KIN) in combination with 0, 0.5 and 1.0 mgl-1 indole-3-butyric acid (IBA). The BA-supplemented media were optimal for both growth and multiplication of shoots. In both groups of genotypes, the highest index of multiplication (IM) was achieved on medium supplemented with 2.0 mgl-1 BA and 1.0 mgl-1 IBA, in R9 early genotype (IM 8.53) and K1 late genotype (IM 10.06). R5 early and in K29 and K75 late genotypes had no multiplication on medium with 1.0 mgl-1 KIN (IM 1.00). Also, in all genotypes the lowest index of multiplication was observed on media supplemented with KIN (without or in combination with IBA).
Key words: cabbage, micropropagation, lateral buds, multiplication index
Corresponding author: Suzana Pavlović, Karadjordjeva 71, Smederevska Palanka, Serbia; phone: +381 26 317 170, fax: +381 26 317 785, e-mail: suzapavlovic@gmail.com
Published in „GENETIKA“ Vol. 44, No.3(2012), pp. 619 -632
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
Dehydrogenase Isoenzyme Polymorphism in genus Prunus, subgenus Cerasus
Slavica Čolić1, Vera Rakonjac2, Milica Fotirić Akšić2, Dragan Nikolić2,
Vladislav OGNJANOV3and Dragan RAHOVIĆ1
1Institute for Science Application in Agriculture, Belgrade, Serbia
2 University of Belgrade, Faculty of Agriculture, Belgrade, Serbia
3University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
Abstract
Čolić S., V. Rakonjac, M. Fortić. Akšić, D.Nikolić, V. Ognjanov, and D. Rahović (2012): Dehydrogenase isoenzyme polymorphism in genus prunus, subgenus cerasus. - Genetika, Vol 44, No. 3,619 -632.
Dehydrogenase polymorphism was studied in 36 sour cherry (Prunus cerasus L.), sweet cherry (Prunus avuim L.), mahaleb (Prunus mahaleb L.), ground cherry (Prunus fruticosa Pall.), duke cherry (Prunus gondounii Redh.), Japanese flowering cherry (Prunus serrulata Lindl.) and four iterspecific hybrids (standard cherry rootstocks ‘Gisela 5’, ‘Gisela 6’, ‘Max Ma’ and ‘Colt’). Inner bark of one-year-old shoots, in dormant stage, was used for enzyme extraction. Vertical PAGE was used for isoenzyme analysis: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), and shikimate dehydrogenase (SDH). All studied systems were polymorphic at 10 loci: Adh -1 (3 genotypes) and Adh-2 (5 genotypes), Fdh-1 (2 genotypes), Gdh-1 (3 genotypes), Idh-1 (4 genotypes) i Idh -2 (5 genotypes), Mdh-1 (3 genotypes), Pgd-1 (4 genotypes), Sdh-1 (1 genotype) i Sdh-2 (3 genotypes). Cluster analysis was used to construct dendrogram on which four groups of similar genotypes were separated. Obtained results indicate that studied enzyme systems can be used for determination of genus Prunus, subgenus Cerasus. Among studied enzyme systems ADH, IDH and SDH were the most polymorphic and most useful to identify genetic variability. Polymorphism of FDH and GDH in genus Prunus, subgenus Cerasus was described first time in this work. First results for dehydrogenase variability of Oblačinska indicate that polymorphism of loci Idh-2 and Sdh-2 can be useful for discrimination of different clones.
Key words: cluster analysis. dehydrogenase, electrophoresis, polymorphism, Prunus spp
.
Corresponding author: Slavica Čolić, Institute for Science Application in Agriculture, Bul. Despota Stefana 68b, 11000 Belgrade, Serbia, phone: ++ 381 11 2751622, fax: ++ 381 11 2752 959, e-mail: slavicacol@yahoo.com
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 633 - 647.
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:635
Original scientific paper
PHENOTYPIC AND GENETIC CHARACTERIZATION OF BOTRYTIS CINEREA ISOLATES FROM TOMATO
Biljana Kuzmanovska1,a, Rade Rusevski1, Ljupcho Jankuloski1, Mirjana Jankulovska1, Dario IVIC2, Katerina Bandzo3
1 Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University, Skopje, Republic of Macedonia
2 Institute for Plant Protection, Croatian Centre for Agriculture, Food and Rural Affairs, Zagreb, Croatia
3Institute of Agriculture, Ss. Cyril and Methodius University, Skopje, Republic of Macedonia
Abstract
Kuzmanovska B., R Rusevski, Lj. Jankuloski, M.Jankulovska, D.Ivic, and K. Bandzo (2012): Phenotypic and genetic characterization of Botrytis cinerea isolates from tomato. - Genetika, Vol 44, No. 3,633 -647.
One hundred and twenty-three isolates of Botrytis cinerea were collected from 7 different areas in the Republic of Macedonia, where tomato is mostly grown in greenhouses and high tunnels. Based on the mycelial formation, intensity of sporulation and sclerotial production, 9 different phenotypes were detected: 4 mycelial and 5 sclerotial. One sclerotial morphological type has not been previously reported. The presence or absence of two transposable elements, boty and flipper, was detected by PCR. Out of 123 isolates, 20 had two transposable elements, boty and flipper (transposa genotype), 48 had neither of these elements (vacuma genotype) and 55 had only the flipper element (flipper genotype). Isolates that contain only boty element were not detected. No relationship between the phenotypes, origin of isolates and the presence/absence of transposable elements, boty and flipper, was found.
Key words: boty, flippe,grey mold, phenotypes,
Corresponding author: Biljana Kuzmanovska,Faculty of Agricultural Sciences and Food,blvd. Aleksandar Mekedonski bb,1000 Skopje, Macedonia,tel: ++389 2 3255 195,fax: ++389 2 3134 310,e-mail: bkuzmanovska@zf.ukim.edu.mk, bilka79@yahoo.com
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 649 -659.
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
EffectS of hybrid on maize grain and plant carbohydrates
Milica RADOSAVLJEVIĆ, Marija MILAŠINOVIĆ-ŠEREMEŠIĆ, Dušanka TERZIĆ, Goran TODOROVIĆ, Zorica PAJIĆ, Milomir FILIPOVIĆ, Željko KAITOVIĆ, Snežana MLADENOVIĆ DRINIĆ
Maize Research Institute, Zemun Polje, Belgrade, Serbia
Abstract
Radosavljević M., M. Milašinović-Šeremešić, D.Terzić, G.Todorović, Z.Pajić, M.Filipović, Ž.Kaitović, and S. Mladenović Drinić (2012): Effects of hybrid on maize grain and plant carbohydrates. - Genetika, Vol 44, No. 3,649 -659.
Maize is one of the most important naturally renewable carbohydrate raw materials. The basic chemical composition (content of starch, protein, oil, crude fibre and ash) and the content of lignocellulose fibres (content of NDF, ADF, ADL, hemicelullose and cellulose) were determined for grain and the whole maize plant of the seven ZP maize hybrids. The negative very significant correlation between protein and starch content (r=-0.78) and significant correlation between oil and starch content (r=-0.65) was obtained in grain. The hybrid ZP 666 had the highest starch, crude fibre, ADF and cellulose content, high NDF content, the lowest ADL and low protein content in grain. The lowest starch, crude fibre, ADF, cellulose content and the highest protein and oil content in grain was determined in hybrid ZP 158. The hybrid ZP 730 had the highest and hybrid ZP158 the lowest dry matter yield of whole plant, whole plant without ear, ear and yield of digestible dry matter of whole plant. The differences in the contents of NDF, ADF, ADL, hemicelluloses, cellulose and digestibility of the whole maize plant among observed ZP hybrids were 6.21%, 4.01%, 0.79%, 5.65%, 3.88% and 6.79%, respectively. Obtained values for the content of lignocellulose fibres differed significantly among hybrids and were closely related to digestibility.
Key words: ADF, ADL, hemicelullose and crude fibres NDF, maize, starch
Corresponding author: Milica Radosavljević, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, Belgrade, phone: +381 11 37 56 704, e-mail address: rmilica@mrizp.rs
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 661– 677.
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
EXAMINATION OF ANTIMICROBIAL POTENTIAL IN NATURAL ISOLATES OF LACTOBACILLUS CASEI/PARACASEI GROUP
Maja TOLINAČKI1, Jelena LOZO1,2, Katarina VELJOVIĆ1, Milan KOJIĆ1,
Đorđe FIRA 1,2 and Ljubiša TOPISIROVIĆ1
1Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Belgrade, Serbia
2Faculty of Biology, University of Belgrade, Belgrade, Serbia
Abstract
Tolinački M.. J. Lozo, K. Veljović, M. Kojić, Đ. Fira and Lj. Topisirović (2012): Examination of antimicrobial potential in natural isolates of Lactobacillus casei/paracasei group. - Genetika, Vol 44, No. 3, 661-677
The aim of this study was to investigate the antimicrobial potential of 52 natural isolates of Lactobacillus casei/paracasei. The incidence of relevant genes encoding BacSJ (bacSJ2-8/bacSJ2-8i gene cluster), acidocin 8912 (acdT), ABC-transporter (abcT) and accessory protein (acc) was also studied. These genes were found to be widespread amongst the analyzed L. casei/paracasei strains. The bacSJ2-8/bacSJ2-8i gene cluster was present in 49 (94.23%) and acdT in 41 (78.85%) of the 52 tested strains. Forty of these strains (76.92%) harbored both analyzed genes. Interestingly, only 17 strains (32.69%) with the bacSJ2-8/bacSJ2-8i gene cluster and/or the acdT gene showed bacteriocin production. Strain L. paracasei BGNK1-62 contained the bacSJ2-8/bacSJ2-8i gene cluster, but did not produce bacteriocin BacSJ possibly due to absence of the abcT and acc genes. Hence, these genes were introduced into BGNK1-62 by transformation with constructed plasmid pA2A, after which BacSJ was produced. In addition, it was found that L. paracasei BGGR2-66 produced new bacteriocin designated as BacGR that was biochemically characterized and its N-terminal sequence was determined.
Key words: Lactobacillus casei/paracasei / BacSJ / acidocin 8912 / ABC-transporter / accessory protein / BacGR
Corresponding author: Maja Tolinački, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia,Tel: +381 11 397 59 60,Fax: +381 11 397 58 08,E-mail: lab6@imgge.bg.ac.rs
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 679 -688.
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.75
Original scientific paper
COMPARISON OF PHYSIOCHEMICAL CHANGES IN TWO POPULAR STRAWBERRY CULTIVARS GROWN IN IRAN (cvs. KURDISTAN & SELVA) DURING STORAGE TIME AT 4 °C
Mohammad JOUKI , Ahmad DADASHPOUR
Young Researchers Club- Islamic Azad University- Shahr-e-qods Branch,
Tehran, I. R. Iran
Abstract
Jouki M., and A. Dadashpour (2012): Comparison of physiochemical changes in two popular strawberry cultivars grown in iran (cvs. Kurdistan & Selva) during storage time at 4 °C. - Genetika, Vol 44, No. 3, 679-688.
In this study the effect of storage time on some quality parameters of two strawberry cultivars fruits storage at 4oC have been evaluated. Several quality parameters were monitored during the storage period. The samples were analyzed 1, 7, 14 and 21days during storage. Fruits were analyzed about fruit weight, sugar content, chromatic parameters L*, a*, b*, fruit firmness, total titrable acidity, pH, and sensory evaluation. The Kurdistan strawberries retained their weight throughout the experiment as opposed to the Selva strawberries which lost 0.6% of their weight per day because of dehydration. The Selva cultivar was exceptionally firmer than Kurdistan strawberries. Two cultivars were rich in vitamin C and sugar but these values were more in Kurdistan cultivar. As expected, Chroma decreased and over the storage time in two cultivars. The results of this study showed that packaged Kurdistan strawberries could prove suitable for 7 days at 4°C compare with 4 days for selva strawberries.
Key words: firmness, quality parameters,strawberry
Corresponding author: Ahmad Dadashpour, Young Researchers Club- Islamic Azad University- Shahr-e-qods Branch, Tehran, I. R. Iran, P.O. Box: 37515-374 Teheran, Iran, email dadashpour@can.ut.ac.ir
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 689 -699
© 2012 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
:
ANALYSIS OF INTER – POPULATION VARIABILITY OF SCOTS PINE (Pinus sylvestris L.) USING MORPHOMETRIC MARKERS
Aleksandar Lučić1, Vasilije Isajev2, Ljubinko Rakonjac1, Milan Mataruga3, Vladan Popović1, Radovan Nevenić1 and Snežana Mladenović Drinić4
1Institute of Forestry, Belgrade, Serbia,
2Univerzity of Belgrade, Faculty of Forestry, Serbia,
3 Univerzity of Banja Luka, Faculty of Forestry, Bosnia and Hercegovina,
4Maize Research Institute, Zemun Polje, Belgrade, Serbia
Abstract
Lučić A., V. Isajev, Lj.Rakonjac, M.Mataruga, V.Popović, R.Nevenić, and S. Mladenović Drinić (2012): Analysis of inter – population variability of Scots pine (Pinus sylvestris L.)using morphometric markers. - Genetika, Vol 44, No. 3, 689-699.
The aim of this study is analysis of inter-population variability of Scots pine (Pinus sylvestris L.) using morphometric parameters of cones and seedlings originating from seven populations in Serbia.The analysis of 1960 cones and their seeds was performed as well as the analysis of morphometric parameters of seedlings (height and root neck diameter) that were produced from this seed. Based on the obtained results the significant differences were noticed in the values of analyzed characters for each population separately. The biological similarity or distance regarding the analyzed characters showed a significant population differentiation. By presenting the preliminary knowledge on the genetic variability of the study populations, the reported results will contribute to better understanding of the significance of the analyzed characters in the population differentiation.
Key words: cones, Scots pine, seeds, seedlings, variability
Corresponding author: Aleksandar Lučić, Institute of forestry, Belgrade, Serbia, phone: ++ 381 63 8413101, emai:l aleksandar.lucic@gmail.com
Published in „GENETIKA“ Vol. 44, No. 3(2012), pp. 701- 710
© 2012 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.1
Original scientific paper
THE USAGE OF MUTANT GENOTYPES IN TOMATO SELECTION FOR SPECIFIC TRAITS AT THE INSTITUTE FOR VEGETABLE CROPS
Jasmina ZDRAVKOVIĆ, Živoslav MARKOVIĆ, Milan ZDRAVKOVIĆ,
Mirjana MIJATOVIĆ, Nenad PAVLOVIĆ
Institute for Vegetable Crops doo, Smederevska Palanka
Abstract
Zdravković J., Ž. Marković, M. Zdravković. M. Mijatović, and N. Pavlović (2012): The usage of mutant genotypes in tomato selection for specific traits at the institute for vegetable crops - Genetika, Vol 44, No. 3, 701-710.
In order to find a superdominant gene for yield, a collection of 350 samples with genes mutations and different expression in different phases of growth and development: fruit size, leaf shape, plant architecture, number of fruits, etc., has been studied (through multi-year research). Current seed production at the Institute for Vegetable Crops in Smederevska Palanka includes 26 hybrids and two in acceptation process, of different purposes and characteristics. The most common mutation genes in tomato selection at the Institute are: sp (self-pruning) inbuilt in determinant hybrids (Balkan F1, Marko F1, Rebus F1) and varieties (Narvik SPF, SP-109, Adonis), u (uniform ripening) - Lido F1, Atina F1, Danubius F1, rin (ripening inhibitor)- Nada F1, Šampion F1, Sef F1, Sidra F1, Rebus F1, j (jointless) – Narvik, Adonis, SP-109, c (potato leaf) - Mi-13, Enigma F1, Rebus F1, Ph -2 (Phytophtora infestans resistance) – Luna F1, Zlatni jubilej F1, Sef F1. Quality of tomato fruits is defined by two groups of genes which determinate the organoleptic traits and nutrition composition. Organoleptic quality includes taste and smell, colour and texture of fruits established in variety differences, nutritive regime of plants, maturity phase and yield. Little is known about gene regulation, responsible for variation of these traits.
Key words: mutant genotypes, specific traits, tomato breeding
Corresponding author: Jasmina Zdravkovic, Institute for Vegetable Crops doo, Karađorđeva 71, Smederevska Palanka, email:jzdravkovic@institut-palanka.co.rs, tel 026/317170,fax 026/317785