GENETIKA, Vol. 45, No. 1 (2013)

GENETIKA, Vol. 45, No. 1 (2013)

Đurđina RUŽIĆ, Tatjana VUJOVIĆ, and Radosav CEROVIĆ

IN VITRO CONSERVATION OF TWO PLANT SPECIES (Prunus cerasifera Ehrh. AND Rubus fruticosus L.) SHOOT TIPS BY ENCAPSULATION DEHYDRATION [Abstract] [Full text]

Gordan ZEC,Milica FOTIRIĆ AKŠIĆ, Slavica ČOLIĆ, Todor VULIĆ, Dragan NIKOLIĆ, Čedo OPARNICA, Zoran JANKOVIĆ

INFLUENCE OF VINEYARD PEACH SELECTIONS ON VIGOUR AND INITIAL YIELD IN PEACH AND NECTARINE [Abstract] [Full text]

Novo PRŽULJ, Vojislava MOMČILOVIĆ, and Jovan CRNOBARAC

PATH COEFFICIENT ANALYSIS OF QUALITY OF TWO-ROW SPRING BARLEY [Abstract] [Full text]

Jela IKANOVIĆ, Vera POPOVIĆ, Vojislav TRKULJA, Ljubiša ŽIVANOVIĆ, Željko LAKIĆ, Slobodanka PAVLOVIĆ

MORPHOLOGICAL CHARACTERISTICS OF THE Interspecies hYbrid BETWEEN SORGHUM AND SUDAN GRASS UNDER INTENSIVE NITROGEN NUTRITION [Abstract] [Full text]

Radica DJEDOVIĆ, Vladan BOGDANOVIĆ, Gligorije TRIFUNOVIĆ, Radmila BESKOROVAJNI, Dragan STANOJEVIĆ

THE EVALUATION OF GENETIC PARAMETERS OF THE TYPE OF CALVING IN THE POPULATION OF HOLSTEIN FRIESIAN COWS [Abstract] [Full text]

Srđan BOJOVIĆ, Philippe HEIZMANN, Dragana DRAŽIĆ, Dragan KOVAČEVIĆ, Petar MARIN, Zorica POPOVIĆ, Rada MATIĆ, Maja JURC

DIVERSITY OF Fraxinus ornus FROM SERBIA AND MONTENEGRO AS REVEALED BY RAPDs [Abstract] [Full text]

Svetlana VOJVODIĆ, Dušica ADEMOVIĆ-SAZDANIĆ, Ivan BUSARČEVIĆ

ASSOCIATION OF HLA -A, -B AND -DRB1 ALLELES WITH HEMATOLOGICAL DISEASES IN VOJVODINA [Abstract] [Full text]

Jelena MARINKOVIĆ, Vuk ĐORĐEVIĆ, Svetlana BALEŠEVIĆ-TUBIĆ, Dragana BJELIĆ, Biljana VUCELIĆ-RADOVIĆ, Dragana JOŠIĆ

Osmotic stress tolerance, PGP traits and RAPD analysis of Bradyrhizobium japonicum strains [Abstract] [Full text]

Slađana ŽILIĆ, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ, Dejan DODIG, Vuk MAKSIMOVIĆ, Vesna KANDIĆ

SOLUBLE FREE PHENOLIC COMPOUND CONTENTS AND ANTIOXIDANT CAPACITY OF BREAD AND DURUM WHEAT GENOTYPES [Abstract] [Full text]

Jelica GVOZDANOVIĆ-VARGA, Mirjana VASIĆ, Janko ČERVENSKI, Anamarija PETROVIĆ and Đorđe MORAVČEVIĆ

Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection [Abstract] [Full text]

Branislav CVJETKOVIĆ, Milan MATARUGA, Vasilije ISAJEV, Jelena LEVIĆ,Aleksandar LUČIĆ, Vojislav TRKULJA, Željka KREMENOVIĆ

VARIABILITY IN GERMINATION AND GERMINATION DYNAMICS OF DIFFERENTLY TREATED SEEDS OF SERBIAN SPRUCE (Picea omorika PANČIĆ/PURKYNĔ) [Abstract] [Full text]

Dubravka JOVICIC,Jelena PAJIC, Boban RAKIC, Ljiljana RADIVOJEVIC, Milos PAJIC, Vaskrsije JANJIC, Aleksandar MILOVANOVIC

CYTOGENETIC BIOMONITORING IN A SERBIAN POPULATION OCCUPATIONALLY EXPOSED TO A COMPLEX MIXTURE OF PESTICIDES [Abstract] [Full text]

Željko DOLIJANOVIĆ, Snežana OLJAČA, Dušan KOVAČEVIĆ, Milena SIMIĆ, Nebojša MOMIROVIĆ and Života JOVANOVIĆ

DEPENDENCE OF the productivity of maize and soybean intercropping systems ON HYBRID TYPE and plant arrangement pattern [Abstract] [Full text]

Jelena VANČETOVIĆ, Dragana IGNJATOVIĆ-MICIĆ, Ana NIKOLIĆ, Sofija BOŽINOVIĆ, Ksenija MARKOVIĆ, Violeta ANĐELKOVIĆ

POTENTIALLY A NEW SUBTYPE OF THE CYTOPLASMIC MALE STERILITY S-TYPE IN MAIZE [Abstract] [Full text]

Nishtman ABDI, Reza DARVISHZADEH, Hamid HATAMI MALEKI

EFFECTIVE SELECTION CRITERIA FOR SCREENING DROUGHT TOLERANT RECOMBINANT INBRED LINES OF SUNFLOWER [Abstract] [Full text]

Gordana SURLAN-MOMIROVIC, Ilona KRÄMER, Kamenko BRATKOVIC, Miroslav ZORIC, Una MOMIROVIC, Gordana BRANKOVIC, Irena CALIC, Vesna KANDIC,Novo PRZULJ, Frank ORDON, Dragan PEROVIC

MOLECULAR CHARACTERIZATION OF BARLEY (Hordeum vulgare L.) ACCESSIONS OF THE SERBIAN GENEBANK BY SSR FINGERPRINTING

[Abstract] [Full text]

Ana SELAMOVSKA, Suzana KRATOVALIEVA, Katerina NIKOLIC

INTERACTION OF ENVIRONMENT CONDITIONS AND GENOTYPES ON EXPRESSION OF GENETIC BACKGROUND IN MICRO-PHENOPHASES OF STRAWBERRY MIXED FLOWER BUD [Abstract] [Full text]

Slađana MARIĆ, Milan LUKIĆ

DETERMINATION OF ETR1 GENOTYPES IN PROMISING APPLE SELECTIONS DEVELOPED AT FRUIT RESEARCH INSTITUTE – ČAČAK [Abstract] [Full text]

Brankica FILIPIC, Branko JOVCIC, Gordana UZELAC, Marija MILJKOVIC, Jelena ANTIC-STANKOVIC, Ljubisa TOPISIROVIC, Natasa GOLIC

OVER-EXPRESSED CmbT MULTIDRUG RESISTANCE TRANSPORTER IMPROVES THE FITNESS OF Lactococcus lactis [Abstract] [Full text]

Nina ILIĆ, Aleksandra KRSTIĆ, Miloš KUZMANOVIĆ, Dragan MIĆIĆ, Nada KONSTANTINIDIS and Marija GUĆ-ŠĆEKIĆ

IDENTIFICATION OF INTRON 1 AND INTRON 22 INVERSIONS OF FACTOR VIII GENE IN SERBIAN PATIENTS WITH HEMOPHILIA A [Abstract] [Full text]

Jana ŽIAROVSKÁ, Eloy C. FERNÁNDEZ, Luigi MILLELA

A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION

[Abstract] [Full text]

Ivana GRUBIŠA, Petar OTAŠEVIĆ, Nebojša DESPOTOVIĆ, Velimir DEDIĆ , Jelena MILAŠIN, Nada VUČINIĆ

GENETIC POLYMORPHISM OF GLUTATHION S-TRANSFERASE P1 (GSTP1) Ile105Val AND SUSCEPTIBILITY TO ATHEROGENESIS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS [Abstract] [Full text]

Dobrivoj POŠTIĆ, Mira STAROVI¹, Tatjana POPOVIĆ, Predrag BOSNIĆ, Aleksandra StanojkoviĆ- SEBIĆ, Radmila PiviĆ, Dragana JOŠIĆ

SELECTION AND RAPD ANALYSIS of Pseudomonas ssp. ISOLATES ABLE TO IMPROVE BIOLOGICAL VIABILITY OF POTATO SEED TUBERS [Abstract] [Full text]

Aleksandar ODALOVIĆ, Ranko PRENKIĆ,Darko DUBAK, Miodrag JOVANČEVIĆ, Miroslav ČIZMOVIĆ, Marija RADUNOVIĆ

EFFECT OF ECOLOGICAL CONDITIONS ON EXPRESSION OF BIOPOMOLOGICAL CHARACTERISTICS OF CHESTNUT (Castanea sativa Mill.) IN NATURAL POPULATIONS OF MONTENEGRO [Abstract] [Full text]

Branka KRESOVIĆ, Vesna DRAGIĆEVIĆ, Boško Gajić, Angelina TAPANAROVA, Borivoj Pejić

THE DEPENDENCE OF MAIZE (Zea mays) HYBRIDS yielding potential ON THE WATER AMOUNTs REACHing THE SOIL SURFACE [Abstract] [Full text]

B.N. SINGH and Seema SISODIA

THE Drosophila ananassae SPECIES COMPLEX: EVOLUTIONARY RELATIONSHIPS AMONG DIFFERENT MEMBERS [Abstract] [Full text]

Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 1-10

Serbia

UDC 575:634
DOI: 10.2298/GENSR1301001R

Original scientific paper

IN VITRO CONSERVATION OF TWO PLANT SPECIES

(Prunus cerasifera Ehrh. AND Rubus fruticosus L.) SHOOT TIPS

BY ENCAPSULATION DEHYDRATION

Đurđina RUŽIĆ¹, Tatjana VUJOVIĆ¹, and Radosav CEROVIĆ²

¹Fruit Research Institute, Čačak, Serbia

²Maize Research Institute „Zemun Polje“, Zemun, Serbia

Abstract

Ruzic Dj., T. Vujovic, and R. Cerovic (2013): In vitro conservation of of two plant species (Prunus cerasifera Ehrh. and Rubus fruticosus L.) shoot tips by encapsulation dehydration. Genetika, Vol 45, No. 1, 1-10.

In vitro grown shoot tips of cherry plum (Prunus cerasifera Ehrh.) and blackberry ‘Čačanska Bestrna’ (Rubus fruticosus L.) were tested for regrowth after cryopreservation using encapsulation dehydration method. Apical, 2–3 mm long shoot tips, were encapsulated in alginate beads composed of 3, 5 and 10% (w/v) alginic acid sodium salt in calcium-free liquid Murashige and Skoog (MS) medium containing 1.0 mg l^-1benzyladenine, 0.1 mg l^-1indole-3-butyric acid and 0.1 mg l^-1gibberellic acid. Polymerization was done in liquid MS medium with 100 mM CaCl₂ for 30 min at room temperature. Encapsulated shoot tips were pre-treated in liquid MS medium with 0.75 or 1 M sucrose for 24 h in growth room and dehydrated for 4 and 8 h (29% and 20% moisture content respectively) before rapid immersion in liquid nitrogen. Upon thawing which involved placing the cryovials in the air current of the laminar airflow cabinet for 2 min, the beads were directly transferred to regrowth medium. In cherry plum, osmotic dehydration in 0.75 M sucrose followed by 8-hour desiccation gave the highest regrowth (60%) of explants encapsulated in 3% and 5% alginate beads. However, in comparison with cherry plum, blackberry displayed significantly lower capacity for regrowth after cryopreservation under described experimental conditions. In this genotype, osmotic dehydration in 1 M sucrose followed by 8-hour desiccation resulted in the highest regrowth (16.7%) of explants encapsulated in 5% alginate beads. Cryopreserved shoot tips of both genotypes multiplied in the three subcultures had normal morphology and similar multiplication capacity in comparison with non-cryopreserved shoots.

Key words: cherry plum, blackberry, cryopreservation, regrowth, multiplication

Corresponding author: Đurđina Ružić, Fruit Research Institute,Kralja Petra I no. 9,32000 Čačak, Serbia,Phone: 032 227 550,Fax: 032 221 391,e-mail: djinaruzic@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 11 -20

Serbia

UDC 575:634.25
DOI:10.2298/GENSR130101Z

Original scientific paper

INFLUENCE OF VINEYARD PEACH SELECTIONS ON VIGOUR AND INITIAL YIELD IN PEACH AND NECTARINE

Gordan ZEC*¹,Milica FOTIRIĆ AKŠIĆ¹, Slavica ČOLIĆ², Todor VULIĆ¹,

Dragan NIKOLIĆ¹, Čedo OPARNICA¹, Zoran JANKOVIĆ³

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²Institute for Science Application in Agriculture, Belgrade, Serbia

³Institute PKB Agroekonomik, Padinska Skela, Serbia

Abstract

Zec G., M. Fotric Akšic, S. Colic, T. Vulic, D. Nikolic, C. Oparnica and Z. Jankovic (2013): Influence of vineyard peach selections on vigour and initial yield in peach and nectarine.Genetika, Vol 45, No. 1, 11-20.

Seedlings (progenies) of eight selected vineyard peach genotypes obtained by self and open pollination were examined in this study during three consecutive years. Two progenies from unselected vineyard peach genotypes with different geographic origin were used as a standard. Peach cv. Autumn Glo and nectarine cv. Stark Red Gold were grafted on F₁ generation of eight vineyard peach selections and two standard progenies. Analysis of variance indicated statistically significant differences in vigour and fruit productivity between fruit trees grafted on different progenies of vineyard peach selections. A high correlation was found between rootstock vigour and fruit production of grafted cultivars. In addition, the principal component analysis made it possible to establish similar groups of rootstocks, depending on its influence to vigour, productivity and indexes of efficiency of grafted peach and nectarine cultivar. The most promising rootstocks for those two cultivars were PSK and 7S because grafted AG and SRG have high fruit weight, initial yields and very satisfactory rootstock, scion and canopy efficiency.

Key words: rootstocks, progeny, seedlings, vigour, initial yield

Corresponding author: Gordan Zec, University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade, Serbia, Tel.+381 112615315/232, e-mail address: zecg2004@yahoo.com,

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 21 - 30

Serbia

UDC 575:633.16
DOI: 10.2298/GENSR1301021P

Original scientific paper

PATH COEFFICIENT ANALYSIS OF QUALITY OF TWO-ROW SPRING BARLEY

Novo PRŽULJ¹, Vojislava MOMČILOVIĆ¹ and Jovan CRNOBARAC²

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agriculture, Novi Sad, Serbia

Abstract

Przulj N., V. Momcilovic, and J. Crnobarac (2013): Path coefficient analysis of quality of two-row spring barley.Genetika, Vol 45, No. 1, 21-30.

Malting quality is composed of numerous interacting traits with a high complexity concerning their biochemical and genetic basis. Malt extract is key indicator of barley malting quality and it is a mega-trait since it is influenced by a number of independent component traits. Understanding genetic and non-genetic factors that effects grain quality and grain yield is crucial in developing new cultivars, seed and mercantile production. Path analysis is one of the reliable statistical techniques which allow separation of the direct effect of each component trait on malt quality from the indirect effects caused by the interdependence component trait. The aim of this study was to investigate spring two-row barley quality as mega-trait depending on the component traits in the conditions of the Pannonian environments. Regression analysis with extract (EXT) as dependant and other traits (yield-YIL, test weight-TW, grain weight-GW, grading-GRA, grain protein concentration-GPC, viscosity-VIS, Kolbach index-KOL, Hartong number-HAR) as independent traits was performed out. Simple coefficient of correlations were calculated between independent traits and EXT in all pair combination and then used as inputs for path coefficient analysis. The quadratic curve fitted the best relationship between EXT and the independent traits. EXT was in positive (P<0.01) relationship with GW, GRA, KOL, and HAR with simple correlation coefficient of 0.47, 0.42, 0.39 and 0.50, respectively and in negative (P<0.01) relationship with GPC and VIS with simple correlation coefficient of -0.72 and -0.51, respectively. Path analysis explained more than 70% of the variation in EXT of which 34.3% was determined by direct negative path coefficient (P<0.01) of GPC without significant any indirect path effect. VIS negatively directly, (P<0.01) and negatively indirectly via GPC effected EXT. KOL did not have significant direct effect on EXT, but had rather prominent indirect effect via GPC, VIS and HAR. HAR positively directly (P<0.01) and positively indirectly via GPC effected EXT. The direct effect of VIS and HAR determined 13.0% and 14.1% of the variation, respectively.

Key words: Barley (Hordeum vulgare L.), malt, extract, quality, path coefficient analysis, correlation, regression

Corresponding author: Novo Pržulj, Institute of Field and Vegetable Crops,Maksima Gorkog 30, 21000 Novi Sad, Serbia,Tel: 021 4898 220, fax: 021 4898 222,e-mail: novo.przulj@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 45, No.1 (2013), pp. 31 -40

Serbia

UDC 575:633.17
DOI: 10.2298/GENSR1301031I

Original scientific paper

MORPHOLOGICAL CHARACTERISTICS OF THE Interspecies hYbrid BETWEEN SORGHUM AND SUDAN GRASS UNDER INTENSIVE NITROGEN NUTRITION

Jela IKANOVIĆ¹, Vera POPOVIĆ ², Vojislav TRKULJA³, Ljubiša ŽIVANOVIĆ¹, Željko LAKIĆ³, Slobodanka PAVLOVIĆ⁴

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²Institute of Field end Vegetable Crops, Novi Sad, Serbia

³University of Banja Luka, Faculty of Agriculture Banja Luka, Bosnia and Herzegovina

⁴NUBL Faculty of Ecology Banja Luka, Bosnia and Herzegovina

Abstract

Ikanovic J., V. Popovic, V. Trkulja, Lj. Živanović, Ž. Lakić and S. Pavlovic (2013): Morphological characteristics of the interspecies hybrid between sorghum and sudan grass under intensive nitrogen nutrition. Genetika, Vol 45, No. 1, 31-40.

The object of this study was a two-year trial (2009 and 2010) regarding variability of morphological characteristics of species belonging to Sorghum genus, more specifically interspecies hybrid between sorghum and Sudan grass Siloking as affected by different applications of nitrogen. The following morphological characteristics were analysed: plant height, number of leaves, leaf ratio, stem ratio, and number of shoots. Samples were taken from the first cut when the effect of the applied nitrogen doses was the strongest. The results showed that increasing nitrogen quantities significantly affected the tested morphological characteristics, especially the intensity of tillering (increased number of secondary stems), number of the formed leaves, and ratio of leaf weight in the total above-ground biomass. The effect of applied nitrogen depended on the weather conditions, i.e. distribution of precipitation, so that plants reached maximum height when 105 kg N ha^-1 was applied in the dry year and 180 kg N ha^-1in the wet year.

Key words: Sorghum, Interspecies hybrid, Genotype, Nitrogen nutrition

Corresponding author: Jela Ikanović, Faculty of Agriculture, Zemun-Belgrade, Tel: +381 11 2615 315; E-mail: jela@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 41 -49

Serbia

UDC 575:636.2
DOI: 10.2298/GENSR1301041D

Original scientific paper

THE EVALUATION OF GENETIC PARAMETERS OF THE TYPE OF CALVING IN THE POPULATION OF HOLSTEIN FRIESIAN COWS

Radica DJEDOVIĆ¹, Vladan BOGDANOVIĆ¹, Gligorije TRIFUNOVIĆ¹,

Radmila BESKOROVAJNI², Dragan STANOJEVIĆ¹

¹University of Belgrade, Faculty of Agriculture, Belgrade –Zemun,Serbia

²Institute PKB Agrieconomic, Belgrade- Padinska Skela, Serbia

Abstract

Djedovic R., V. Bogdanovic, G. Trifunovic, R. Beskorovajni, and D. Stanojevic (2013): The evaluation of genetic parameters of the type of calving in the population of holstein friesian cow. Genetika, Vol 45, No. 1, 41-49.

Genetic parameters (heritability coefficients and genetic correlations) of the type of calving, number of stillbirths and birth weight have been evaluated in the population of Holstein Friesian cattle breed. Data sets have been analysed by means of the Mixed Least Square Model (LSMLMW). Besides a random effect of bull-sires, the model has also included the fixed effects of farm, season, sex, the evaluation of viability of calves and types of birth. Estimated heritability values and heritability errors for the type of calving (TC), number of stillbirths (SB) and birth weight (BWT) were low: 0.190 ± 0.062; 0.018 ± 0.006 and 0.149 ± 0.051, respectively. Heritabilities of the analysed traits were evaluated on the grounds of the calves` bull-sires additive value (direct heritability). The values of the genetic correlation coefficients between examined traits ranged from -0.251 (correlation between the type of calving and number of stillbirths) to 0.340 (correlation between the number of stillbirths and birth weight).

Key words: type of calving, number of stillbirths, birth weight, genetic parameters, Holstein- Friesian breed

Corresponding author: Radica Djedović, University of Belgrade, Faculty of Agriculture, 11080 Zemun; email:genrad@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 51 -61

Serbia

UDC 575:630
DOI: 10.2298/GENSR1301051B

Original scientific paper

DIVERSITY OF FRAXINUS ORNUS FROM SERBIA AND MONTENEGRO

AS REVEALED BY RAPDs

Srđan BOJOVIĆ¹, Philippe HEIZMANN², Dragana DRAŽIĆ³, Dragan KOVAČEVIĆ⁴,

Petar MARIN⁵, Zorica POPOVIĆ¹, Rada MATIĆ¹, Maja JURC⁶

¹University of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia

²Université Claude Bernard Lyon 1, Génome et Evolution des Plantes Supérieures, France

³Institute of Forestry, Belgrade, Serbia

⁴Maize Research Institute “Zemun Polje”, Zemun-Belgrade

⁵University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“,Belgrade, Serbia

⁶University of Ljubljana, Biotechnical Faculty, Department of Forestry and Renewable Forest Resources, Ljubljana, Slovenia

Abstract

Bojovic S., P. Heizmann, D. Drazic, D.Kovacevic, P.Marin, Z. Popovic, R.Matic and M. Jurc (2013): Diversity of Fraxinus ornus from Serbia and Montenegro as revealed by RAPDs .Genetika, Vol 45, No. 1, 51-62.

PCR-RAPD markers revealed individual variation in F. ornus. A total of 122 fragments were amplified using 7 primers and of these 97 fragments were polymorphic. The percentage of polymorphic loci was between 53.3% and 74.6% with an average of 63.1%. The mean gene diversity for all populations was 0.30 and the mean Shannon’s index was 0.44. Of the total genetic variation 87% was intra-population whilst 13% was inter-population. The Mantel test revealed significant correlation between genetic and geographical distance matrice. Results herein represent the first use of molecular genetic (DNA) markers to characterize genetic variation in F. ornus populations. The partition of total genetic variance indicates a relatively restricted population differentiation as expected in outcrossing species. Present and future information on genetic structure and variability in F. ornus needs to be incorporated into strategies for the preservation of genetic resources of tree species.

Key words: Manna ash, genetic variance, RAPD

Corresponding author: Dr. Srđan Bojović, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia, email :bojovic@ibiss.bg.ac.rs, tel. +381112078365, fax. +381112761433

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 63 -74

Serbia

UDC 575
DOI: 10.2298/GENSR1301063V

Original scientific paper

ASSOCIATION OF HLA -A, -B AND -DRB1 ALLELES WITH HEMATOLOGICAL DISEASES IN VOJVODINA

Svetlana VOJVODIĆ¹, Dušica ADEMOVIĆ-SAZDANIĆ¹, Ivan BUSARČEVIĆ²

¹Institute for Blood Transfusion of Vojvodina, Department for laboratory testing,

Tissue Typing Compartment, Novi Sad, Serbia

²University of Novi Sad, Medical Faculty Novi Sad, Novi Sad, Serbia

Abstract

Vojvodić S., D. Ademović-Sazdanić, and I. Busarčević (2013): Association of Hla -A, -B and - DRB1 alleles with hematological diseases in Vojvodina. Genetika, Vol 45, No. 1, 63-74.

Major histocompatibility complex (MHC) genes are involved in various mechanisms of pathogenesis and immunoediting of hematological diseases. This study aimed to investigate the association between HLA -A, -B and -DRB1 alleles with hematological diseases. In this study, we performed DNA-based HLA typing by polymerase chain reaction analysis with sequence-specific primers (PCR-SSP) to distinguish HLA-A, -B, and -DRB1 alleles. Eighty-two patients with hematological diseases (29 with acute lymphoblastic leukemia (ALL), 19 with acute nonlymphoblastic leukemia (ANLL), 5 with chronic myelogenous leukemia (CML), 2 with chronic lymphocytic leukemia (CLL), 9 with myelodysplastic syndrome (MDS), 9 with lymphomas (M.Hodgkin (HL) and non-Hodgkin (NHL)), 7 with aplastic anemia (AA) and 2 with multiple myeloma (MM)), were included in the study and compared with 111 healthy blood donors, residents from Vojvodina, evaluating the strength of the observed associations by measuring the aetiologic and preventive fractions. Among the alleles significantly associated with hematological diseases, HLA-A*24 showed an aetiologic fraction higher than those of HLA-A*26 and A*25 (RR=1.027, EF=1.233, RR=1.047, EF=1.141 and RR=1.213, EF=0.910).Negative association with significant preventive fraction was observed with HLA-B*18 and HLA-DRB1*11 alleles, with RR=0.400, PF=0.179 and RR=0.587, PF=0.176. Our results suggest that HLA-A*24, A*26 and A*25 as associated more frequently than other specificities with a hypothetical disease predisposing genes, may play a role in the pathogenesis of hematological diseases.

Key words: Human Leukocyte Antigens; hematological diseases; association.

Corresponding author: Svetlana Vojvodić, Institute for Blood Transfusion of Vojvodina, Hajduk Veljkova 9a, 21000 Novi Sad, Tel.: +381 21 4877 963; fax: +381 21 4877 978, e-mail: svetlana. vojvodic021@gmail.com

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Published in „GENETIKA“ Vol. 45, No.1 (2013), pp. 75 -86

Serbia

UDC 575
DOI: :10.2298/GENSR1301075M

Original scientific paper

Osmotic stress tolerance, PGP traits and RAPD analysis

of Bradyrhizobium japonicum strains

Jelena MARINKOVIĆ¹, Vuk ĐORĐEVIĆ¹, Svetlana BALEŠEVIĆ-TUBIĆ ¹,

Dragana BJELIĆ¹, Biljana VUCELIĆ-RADOVIĆ ², Dragana JOŠIĆ ³

¹ Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Belgrade, Faculty of Agriculture, Belgrade-Zemun, Serbia

³ Institute of Soil Science, Belgrade, Serbia

Abstract

Marinkovic J., V. Djordjevic, S.Baleševic Tubic, D.Bjelic, B.Vucelic-Radovic and D. Josic (2013): Osmotic stress tolerance, PGP traits and RAPD analysis of Bradyrhizobium japonicum strains. Genetika, Vol 45, No. 1, 75-86.

The osmotic stress tolerance of B. japonicum strains assessed according to their persistence in PEG solution. The lowest tolerance to osmotic stress was observed in strain 511 (43.3%), and the highest tolerance was observed for strain D216 (3.3% growth reduction in presence of PEG). PGP traits of B. japonicum strains were tested. None of five B. japonicum strains produced siderophore, strains 511 and 518 had the urease ability, and only B. japonicum 518 strain showed the ability to solubilize insoluble tricalcium phosphate. RAPD analysis, using AP10, BC318, AF14 and SPH1 primers, indicated genetic differences between Bradyrhizobium strains. The first group (strains 3, 6 and 518) showed more than 80% similarity. Strains 511 and D216 formed separate clusters. Difference between strains D216 and the other strains were more than 60%, with maximum value of 72% in comparison with strain 511. Plant-growth promoting (PGP) traits, osmotic stress tolerance and RAPD analysis highlighted strain D216 as useful for further investigation of B. japonicum impact on drought reduction in symbiosis with soybean.

Key words: Bradyrhizobium japonicum, osmotic stress tolerance, RAPD analysis, PGPR

Corresponding author: Marinković Jelena,Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia,Tel.: 021/4898-471,Fax: 021/4898-455,e-mail: jelena.marinkovic@nsseme.com

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Published in „GENETIKA“ Vol. 45, No.1 (2013), pp. 87-100

Serbia

UDC 575:633.11
DOI: 10.2298/GENSR1301087Z

Original scientific paper

SOLUBLE FREE PHENOLIC COMPOUND CONTENTS AND ANTIOXIDANT CAPACITY OF BREAD AND DURUM WHEAT GENOTYPES

Slađana ŽILIĆ¹, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ², Dejan DODIG¹, Vuk MAKSIMOVIĆ², Vesna KANDIĆ¹

¹Maize Research Institute, “Zemun Polje”, Belgrade-Zemun, Serbia

²Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia

Abstract

Zilic S., V. Hadzi –Taskovic Šukalovic, D. Dodig, V.Maksimovic and V. Kandic (2013): Free soluble phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes. Genetika, Vol 45, No. 1, 87-100.

The objective of this study was to determine phenolic compounds and the total antioxidant capacity in the grain of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) wheat genotypes. Soluble free forms of total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids were investigated. In addition, the correlation coefficients between total antioxidant capacities and the concentration of different soluble free phenolic compounds, as well as between soluble free total phenolics and phenolic acids, flavonoids and PVPP bound phenolics were determined. Significant differences in the content of aceton/water extractable total phenolics, PVPP bound phenolics and phenolic acids between and within two wheat species were found. On the average, durum wheat samples had about 1.19-fold higher total phenolic compounds and about 1.5-fold higher PVPP bound phenolics than bread wheat samples. Three phenolic acids, ferulic, caffeic and chlorogenic, were detected in wholemeal bread wheat. Caffeic acid was not found in durum wheat samples whilst ferulic acid was the most abundant. Proanthocyanidins in bread and durum wheat genotypes were not detected. The antioxidant capacity measured as the DPPH radical scavenging activity was similar in wholemeal of bread and durum wheat, however, significant differences were observed among genotypes within species.

Key words: bread and durum wheat, phenolic compounds, antioxidant capacity.

Corresponding author: Slađana Žilić, Maize Research Institute, “Zemun Polje”, Department of Technology, Slobodana Bajića 1, 11000 Belgrade-Zemun, Serbia; tel.:+381113756704; fax: +381113754994; E-mail: szilic@mrizp.rs

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Published in „GENETIKA“ Vol. 45, No. 1 (2013), pp. 101 - 108

Serbia

UDC 575:635.25

DOI: 10.2298/GENSR1301101G

Original scientific paper

fenotipske razlike u osnovnim karakteristikama kolekcije genotipova crnog luka iz Srbije

Jelica GVOZDANOVIĆ-VARGA¹, Mirjana VASIĆ¹, Janko ČERVENSKI¹,

Anamarija PETROVIĆ¹ i Đorđe MORAVČEVIĆ²

¹Institut za ratarstvo i povrtarstvo, Novi Sad, Srbija

² Poljoprivredni fakultet, Univerzitet u Beogradu, Srbija

Abstract

Gvozdanović-Varga J., M. Vasic, J. Cervenski, A. Petrovic, and Đ. Moravcevic (2013): Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection - Genetika, vol 45., No.1,101-108.

The onion is a widely distributed vegetable crop, which takes an important place in the vegetable production in Serbia. The traditional planting method is the one from sets. Old cultivars and populations and, in recent years, foreign cultivars are grown. The large variety of genotypes, including both domestic populations and cultivars, comprises the significant gene pool of this region. The onion collection of the Institute of Field and Vegetable Crops in Novi Sad is based on the populations and cultivars from the territory of the former Yugoslavia. This paper reviews 30 onion genotypes on the basic IPGRI descriptors (ANNEX I). Variability of the reviewed characteristics was determined by PC analysis. High variability values have been established for bulb skin color, bulb flesh color, bulb hearting and bulb skin thickness. The genotypes varied in bulb skin color as well as in bulb flesh color from white to dark violet. These two characteristics had the largest impact on clustering, with a single genotype being heterogeneous exactly for these two characteristics.

Key words: hierarchical clustering, onion, PC analysis

Corresponding author: Jelica Gvozdanović-Varga, Institute of field and vegetable crops, M.Gorkog 30, 21000 Novi Sad, Serbia, tel: 021-4898-100, e-mail: jelica.varga@nsseme.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 109 - 119

Serbia

UDC 575:630
DOI: 10.2298/GENSR1301109C

Original scientific paper

VARIABILITY IN GERMINATION AND GERMINATION DYNAMICS OF DIFFERENTLY TREATED SEEDS OF SERBIAN SPRUCE (PICEA OMORIKA PANČIĆ/PURKYNĔ)

Branislav CVJETKOVIĆ¹, Milan MATARUGA¹, Vasilije ISAJEV², Jelena LEVIĆ³,

Aleksandar LUČIĆ⁴, Vojislav TRKULJA⁵, Željka KREMENOVIĆ⁶

¹Faculty of Forestry, University of Banja Luka, Bosnia and Herzegovina

²Faculty of Forestry, University of Belgrade, Republic of Serbia

³Maize Research Institute, Zemun, Republic of Serbia

⁴Institute of Forestry, Belgrade, Republic of Serbia

⁵Faculty of Agriculture, University of Banja Luka, Bosnia and Herzegovina

⁶Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia and Herzegovina

Abstract

Cvetkovic B., M. Mataruga, V. Isajev, J. Levic, A. Lucic, V. Trkulja, and Z. Kremenovic (2013): Variability in germination and germination dynamics of differently treated seeds of Serbian spruce (Picea omorika Pančić/Purkynĕ) .Genetika, Vol 45, No. 1, 109-119.

Genetic-physiological approach was used in the study of the quality of Serbian spruce seed (Picea omorika /Pančić/Purkynĕ), collected in the populations on the left bank of the river Drina. The seed originated from the three populations that represent the overall ecological and productive conditions of Serbian spruce populations in Bosnia and Herzegovina. Two natural populations were selected: Veliki Stolac and Gostilj, and one planted forest population in Srebrenica. The cones were collected in late autumn 2009 and early spring 2010. They were collected from five trees from Srebrenica and Gostilj, and seven trees from Veliki Stolac. Immediately after they were collected, the cones and seeds were processed and germination tests were done. Germination, germination viability and germination dynamics were studied in three categories of seed treatment: 1) control unit (just processed seed), 2) seed stored for six months at 0-4 ºC, 3) seed stored for seven months at 0-4º C and treated with fungicide after five months of storing. The number of germinated seed was observed and noted on the third, fourth, fifth, seventh, tenth, fourteenth, twenty-first and twenty-eighth day.There were significant differences in germination dynamics at the population level and the level of treatment during the first couple of days of germination test. Fungicide (captan) acted as an inhibitor on seed germination process. The seeds originating from the largest population of Veliki Stolac showed the best response to storing treatment with fungicide related to germination dynamics.The seed originating from Srebrenica andkept at low temperatures 0-4 °C without treatment with fungicides showed the best result in germination. Regardless of the presence of differences in the dynamics of germination, significant differences in germination regarding different treatments on the last day of the test, were not recorded.

Key words: Serbian spruce, seed treatment, germination, germination dynamics

Corresponding author: Branislav Cvjetković, Faculty of Forestry, Stepe Stepanovića 75a, University of Banja Luka, Bosnia and Herzegovina, tel. + 387 51 460 550, email: cvjetkovicb@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 121 -133

Serbia

UDC 575
DOI: 10.2298/GENSR1301121J

Original scientific paper

CYTOGENETIC BIOMONITORING IN A SERBIAN POPULATION OCCUPATIONALLY EXPOSED TO A COMPLEX MIXTURE OF PESTICIDES

Dubravka JOVICIC ¹,Jelena PAJIC ², Boban RAKIC², Ljiljana RADIVOJEVIC ³, Milos PAJIC⁴, Vaskrsije JANJIC³, Aleksandar MILOVANOVIC²

¹Singidunum University, Faculty of Applied Ecology (FUTURA), Belgrade, Serbia

²Serbian Institute of Occupational Health “Dr Dragomir Karajović“, Belgrade, Serbia

³Institute of Pesticides and Environmental Protection, Belgrade, Serbia

⁴University of Belgrade, Faculty of Agriculture, Serbia

Abstract

Jovicic D., J. Pajic, B. Rakic, Lj. Radivojevic, M. Pajic, V. Janic and A. Milovanovic (2013): Cytogenetic biomonitoring in a Serbian population occupationally exposed to a complex mixture of pesticides. Genetika, Vol 45, No. 1, 121-133.

The aim of this study was the analysis of chromosomal aberrations (CA) and premature centromeric division (PCD) in people exposed to pesticides at work. The research included 26 subjects occupationally exposed to pesticides, of average age 39.89 + 8.66, and 32 control subjects of average age 40.57 + 6.57. Mann-Whitney U tests showed statistically significant differences between the groups for mean values of all the examined variables. In addition, Spearman’s (non-parametric) correlation test detected a positive linear correlation between CA and PCD. The presence of mostly chromatid and isochromatid breaks in the examinees indicates continuous exposure to pesticides. The absence of complex chromosomal rearrangements points to adequate protection of the subjects at their work places.

Key words: Occupational exposure, Human lymphocytes, chromosomal aberrations, pesticides

Corresponding author: Jelena Pajic;Deligradska 29, 11 000 Beograd, tel: +381 11 34 00 976; fax: +381 11 26 43 675,e-mail: jejapajic@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 135 -144

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1301135D

Original scientific paper

DEPENDENCE OF the productivity of maize and soybean intercropping systems ON HYBRID TYPE and plant arrangement pattern

Željko DOLIJANOVIĆ^*1, Snežana OLJAČA¹, Dušan KOVAČEVIĆ¹, Milena SIMIĆ², Nebojša MOMIROVIĆ¹ and Života JOVANOVIĆ²

¹University of Belgrade, Faculty of Agriculture, Institute of Field and Vegetable crops, Belgrade-Zemun, Serbia

²Maize Research Institute, Zemun Polje, Belgrade-Zemun, Serbia

Abstract

Dolijanović Ž., S. Oljača, D. Kovačević, M. Simić, N. Momirović and Ž. Jovanović (2013): Dependence of the productivity of maize and soybean intercropping systems on hybrid type and plant arrangement pattern.Genetika, Vol 45, No. 1, 135-144.

Intercropping systems could improve utilization of the most important resources (soil, water and nutrients), provide a better control of weeds, pests and diseases, and finally higher productivity, especially under rain-fed growing conditions. This study aimed to determine the effects of three maize (Zea mays L.) prolific hybrids (FAO 500, 600 and 700) and the spatial intercrop patterns on the above-ground biomass and grain yields of maize and soybean (Glycine max L. Merrill), on chernozem soil type at Zemun Polje, Belgrade, in 2003, 2004 and 2005. The experimental design was a complete randomized block with four replications and three treatments: 3 rows of maize and 3 rows of soybean in strips for each maize hybrid (three variants, 3 rows of maize and 3 rows of soybean in alternate rows for each hybrid (another three variants) and monocrops of both maize and soybeans. To optimize the ecological and economic benefits of maize/soybean intercrop in terms of yield, variety selection and compatibility of the component crops should be made using established agronomic management practices involving the two crops. Suitable maize varieties for maize/soybean intercrop systems are varieties that have less dense canopy. These varieties would therefore have lesser shading effect to the understory beans. However, establishment of an appropriate spatial arrangement of the component crops would be essential to alleviate negative effects especially on the less competitive crop.The intercropping system in alternate rows showed significantly higher above-ground biomass and grain yields in comparation with both the strip intercropping system and maize monocrops in 2004. Soybean gave significantly lower above-ground biomass and grain yield in intercrops than in monocrops. Maize prolific hybrid growing in intercropping with soybean as legume crop, increased productivity of cropping system, especially in favourable agroecological conditions.Maize and soybean yields reduction could have also been due to inter-specific competition for resources such as nutrients and water. Moreover, performance of the currently grown varieties in the semi-arid regions depends on the amount of rainfall received during the growing season.

Key words: maize prolific hybrids, above-ground biomass, grain yield, intercrops and monocrops

Corresponding author: Željko Dolijanović, University of Belgrade, Faculty of Agriculture, Institute of Field and Vegetable crops, dolijan@agrif.bg.ac.rs; +381 11 2615-315 ext. 321, fax: +381 11 3161-987

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Published in „GENETIKA“ Vol. 45, No.1(2013), pp. 145 - 151

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1301145V

Original scientific paper

POTENTIALLY A NEW SUBTYPE OF THE CYTOPLASMIC MALE STERILITY S-TYPE IN MAIZE

Jelena VANČETOVIĆ, Dragana IGNJATOVIĆ-MICIĆ*, Ana NIKOLIĆ,

Sofija BOŽINOVIĆ, Ksenija MARKOVIĆ, Violeta ANĐELKOVIĆ

Maize Research Institute Zemun Polje ,Zemun Polje – Belgrade,Serbia

Abstract

Vancetovic J., D. Ignjatovic Micic, A. Nikolic, S. Bozinovic, K.Markovic and V. Andjelkovic (2013): Potentially a new subtype of the cytoplasmic male sterility S-type in maize. Genetika, Vol 45, No. 1, 145-151.

In gene-bank maize collection of Maize Research Institute Zemun Polje (MRI) two samples with untypical mtDNA profile for cytoplasmic male sterility (cms) were identified. These two samples showed typical multiplex polymerase chain reaction (PCR) band for cms-S, but also an additional band of unknown nature. It is assumed that the additional band is the result of a rearrangement of the two mitochondrial episomes characteristic for the cms-S in maize or a duplication of the part of cms-S mitochondrial genome. Additional field and laboratory experiments are necessary in the further lightening of this phenomenon.

Key words: cytoplasmic male sterility, episom, multiplex PCR reaction, MRI gene-bank

Corresponding author: : Dragana Ignjatovic Micic, Maize Research Institute S. Bajica 1, Belgrade, Serbia, email: idragana@mrizp.rs, phone: + 381 11 37 56 627mob: +381 64 840 6029,fax: +381 11 37 56 707

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 153 - 166

Serbia

UDC 575:633

DOI: 10.2298/GENSR1301153A

Original scientific paper

EFFECTIVE SELECTION CRITERIA FOR SCREENING DROUGHT TOLERANT RECOMBINANT INBRED LINES OF SUNFLOWER

Nishtman ABDI¹, Reza DARVISHZADEH^1,2, Hamid HATAMI MALEKI³

^{^[1]}Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran.

²Institue of Biotechnology, Urmia University, Urmia, Iran.

³Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.

Abstract

Abdi N., R. Darvishzadeh, and H.H. Maleki (2013): Effective selection criteria for screening drought tolerant recombinant inbred lines of sunflower.) .Genetika, Vol 45, No. 1, 153-166.

In this study, seventy two sunflower recombinant inbred lines were tested for their yielding ability under both water-stressed and well-watered states. The inbred lines were evaluated in a rectangular 8´9 lattice design with two replications in both well-watered and water-stressed conditions, separately. Eight drought tolerance indices including stability tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress susceptibility index (SSI), tolerance index (TOL), yield index (YI) and yield stability index (YSI) were calculated based on grain yield for every genotype. Results showed the highest values of mean productivity (MP) index, geometric mean productivity (GMP), yield index (YI), harmonic mean (HM) and stress tolerance index (STI) indices for ‘C134a’ inbred line and least values of stress susceptibility index (SSI) and tolerance (TOL) for C61 inbred line. According to correlation of indices with yield performance under both drought stress and non-stress states and principle component analysis, indices including HM, MP, GMP and STI could properly distinguish drought tolerant sunflower inbred lines with high yield performance under both states. Cluster analysis of inbred lines using Y_s, Y_p and eight indices, categorized them into four groups including 19, 6, 26 and 19 inbred lines.

Key words: Sunflower, drought tolerance indices, principle component analysis, cluster analysis.

Corresponding author: Dr. R. Darvishzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran. E-mail: r.darvishzadeh@mail.urmia.ac.ir. Phone: + 98 441 2972785. Fax: + 98 441 2779558

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 167- 180

Serbia

UDC 575:633.16
DOI: 10.2298/GENSR1301167S

Original scientific paper

MOLECULAR CHARACTERIZATION OF BARLEY (HORDEUM VULGARE L.) ACCESSIONS OF THE SERBIAN GENEBANK BY SSR FINGERPRINTING

Gordana SURLAN-MOMIROVIC¹, Ilona KRÄMER², Kamenko BRATKOVIC³, Miroslav ZORIC⁴, Una MOMIROVIC⁵, Gordana BRANKOVIC¹, Irena CALIC¹, Vesna KANDIC⁶,

Novo PRZULJ⁴, Frank ORDON², Dragan PEROVIC^2*

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

² Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Quedlinburg, Germany

³Centre for Small Grains, Kragujevac, Serbia

⁴ Institute of Field and Vegetable Crops, Novi Sad, Serbia

⁵ State University of Novi Pazar, Serbia

Abstract

Surlan Momirovic G., I. Kramer, K.Bratkovic, M.Zoric, U. Momirovic, G.Brankovic, I.Calic, V.Kandic, N.Przulj, F. Ordon and D. Perovic (2013): Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian genebank by SSR fingerprinting. Genetika, Vol 45, No. 1, 167-180.

Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two-rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P < 0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (D_SA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes.

Key words: barley diversity, Serbian Gene Bank, SSRs, population structure

Corresponding author: Dragan Perovic, Julius Kuehn-Institute, Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany,e-mail: dragan.perovic@jki.bund.de,Tel.: + 49 (0)394647611

Fax: + 49 (0)394647600

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 181-188

Serbia

UDC 575:634.75
DOI: 10.2298/GENSR1301181S

Original scientific paper

INTERACTION OF ENVIRONMENT CONDITIONS AND GENOTYPES ON EXPRESSION OF GENETIC BACKGROUND IN MICRO-PHENOPHASES OF STRAWBERRY MIXED FLOWER BUD

Ana SELAMOVSKA¹, Suzana KRATOVALIEVA¹, Katerina NIKOLIC²

¹Institute of Agriculture, University Ss. Cyril and Methodius, Skopje, Republic of Macedonia

²Faculty of Agriculture, Lesak, Serbia

Abstract

Selamovska A., S. Kratovalieva, and K. Nikolic (2013): Interaction of environment conditions and genotypes on expression of genetic background in micro-phenophases of strawberry mixed flower bud. Genetika, Vol 45, No. 1, 181-188.

The aim of this research is differentiation or micro-phenophases of reproductive organs on two junebearing strawberry (Fragaria x anannassa) varieties senga sengana and pocahontas, depending on climate conditions, rosettes ordering and cultivate manner (orchard mulched on black foil and orchard on bare soil). The beginning of differentiation of flower buds is genetic characteristic depending on climate conditions (insulations, day length, higher midday and night air temperatures from 1.05 till the beginning of differentiation, the sum of rainfalls from the beginning of May until the end of July), order of rosettes and cultivate manner The sum of effective temperatures over 10^oC from 1^st of May till the beginning of differentiation has no influence on beginning of flower buds differentiation. First morphological changes of the apical meristem were started in the first decade of August that has coincided with the day length of 14 hours and day insulations of 9.3 hours. Micro-phenophases were undergoing almost at the same time in both varieties, only the beginning at pocahontas was 2-3 days earlier. Primary rosettes differ 10-15 days earlier than the secondary rosettes. Plants that grown on black foil had 7-10 days earlier flower bud differentiation compared to those grown on bare soil.

Key words: strawberry, mixed flower buds, differentiation, micro-phenophases.

Corresponding author: Ana Selamovska, Institute of Agriculture, Aleksandar Makedonski bb, 1000 Skopje, Phone/fax +38923230910/+38923114283,E-mail: anfilika2@t-home.mk

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 189-196

Serbia

UDC 575:634.11
DOI: 10.2298/GENSR1301189M

Original scientific paper

DETERMINATION OF ETR1 GENOTYPES IN PROMISING APPLE SELECTIONS DEVELOPED AT FRUIT RESEARCH INSTITUTE – ČAČAK

Slađana MARIĆ, Milan LUKIĆ

Fruit Research Institute – Čačak

Abstract

Maric S., and M. Lukic (2013): Determination of ETR1 genotypes in promising apple selections developed at Fruit Research Institute – Čačak. Genetika, Vol 45, No. 1,189-196.

Ethylene, as the simplest signaling molecule with hormone-like function, regulates a broad spectrum of different processes in plants, including ripening. It is perceived by a receptor family divided into two subfamilies (I and II). In apple, ETR1 gene encodes one of ethylene receptors − ETR1 receptor which is a member of subfamily I. ETR1 genotypes were determined for six promising apple selections bred at Fruit Research Institute in Čačak [J/54/53/59 (‘Cox's Orange Pippin’ O.P.), J/1/7, J/1/20, J/2/14 and J/60/7/63 (‘Granny Smith’ × ‘Golden Delicious’), and J/2/50 (‘Idared’ O.P.)] and four commercially important parental cultivars (‘Cox's Orange Pippin’, ‘Golden Delicious’, ‘Granny Smith’ and ‘Idared’). Polymorphism of ETR1 gene was detected by restriction analysis of PCR amplified product with two restriction enzymes (RsaI and AluI). Three alleles (a, b and c) and four allelic constitutions of ETR1 gene (aa, ac, b,a/c and c,a/c) were detected. This study has confirmed that ETR1 gene is inherited in Mendelian fashion and showed that polymorphism of ETR1 gene can aid cultivar and selection genotyping. Based on allelic constitution of genes involved in ethylene biosynthesis and perception, and on the major biological and agronomic traits, J/54/53/59 has been singled out as elite apple selection.

Key words: ETR1 gene, apple, selection, ethylene

Corresponding author: Slađana Marić, Fruit Research Institute, Kralja Petra I/9, 32000 Čačak, Serbia, Phone: ++381 32 221 375, Fax: ++381 32 221 391, e-mail: nidzovicsladja@yahoo.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 197-206.

Serbia

UDC 575
DOI: 10.2298/GENSR1301197F

Original scientific paper

OVER-EXPRESSED CmbT MULTIDRUG RESISTANCE TRANSPORTER IMPROVES THE FITNESS OF Lactococcus lactis

Brankica FILIPIC^1,2, Branko JOVCIC^1,3, Gordana UZELAC¹, Marija MILJKOVIC¹, Jelena ANTIC-STANKOVIC², Ljubisa TOPISIROVIC¹, Natasa GOLIC^1*

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

²Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

³Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Filipic B, B. Jovcic, G. Uzelac, M.Miljkovic, J.Antic-Stankovic, Lj.Topisirovic and N. Golic (2013): Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis. Genetika, Vol 45, No. 1, 197-206.

The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell.

Key words: Lactococcus lactis, bacterial fitness, multidrug resistance

Corresponding author: Natasa Golic, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia, natasag@imgge.bg.ac.rs; lab6@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 207-216.

Serbia

UDC 575
DOI: 10.2298/GENSR1301207I

Original scientific paper

IDENTIFICATION OF INTRON 1 AND INTRON 22 INVERSIONS OF FACTOR VIII GENE IN SERBIAN PATIENTS WITH HEMOPHILIA A

Nina ILIĆ ¹, Aleksandra KRSTIĆ ¹, Miloš KUZMANOVIĆ ¹, Dragan MIĆIĆ¹, Nada KONSTANTINIDIS ² and Marija GUĆ-ŠĆEKIĆ ¹

¹ Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Belgrade, Serbia

²Institute for Child and Youth Health Care of Vojvodina, Novi Sad

Abstract

Ilic N., A. Krstic, M.Kuzmanovic, D. Micic, N.Konstantinidis, and M. Guc-Scekic (2013): Identification of intron 1 and intron 22 inversions of factor VIII gene in Serbian patients with hemophilia A. Genetika, Vol 45, No. 1, 207-216.

Hemophilia A (HA) is a common X-linked recessive bleeding disease caused by mutations of FVIII gene. Inversion of intron 1 (inv1) and intron 22 (inv22) are recurrent mutations in severe HA, causing 50% of cases. Inv1 has been reported to occur in 2–5% and inv 22 in 45% of severe HA patients. Our objective was to determine, for the first time in Serbia, the frequency of inv1 and inv22 in a group of severe HA patients and to compare these data with those from other countries. Study subjects were 50 HA patients, diagnosed and treated from April 2009 to June 2012 at Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic” (IHS) and Institute for Child and Youth Health Care of Vojvodina (IHV).The presence of inv1 and inv22 was analyzed using Inverse shifting PCR (IS-PCR). Our results revealed that the frequencies of inv1 and inv22 in the cohort of Serbian patients were 6 % and 42% (34% of inv22 type I and 8% of inv22 type II) respectively . These frequencies were in line with those found in other populations. Carrier status analyses of 65 family members (mothers and sisters) showed the de novo inversion of intron 22 in one patient. Genetic Counseling Units of IHS and IHV provide the adequate genetic advice to all HA affected patients and their family members.

Key words: hemophilia A, intron 1 inversion, intron 22 inversion, frequency, Serbia

Corresponding author: Nina Ilić, Laboratory for Medical Genetics, Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Radoja Dakica 6-8, 11070 Belgrade, phone: ++ 381 11 3108 273; mob. phone: ++ 381 64 1366 995; e-mail: genetikaimd@beotel.rs, lakic.nina@gmail.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 217-226

Serbia

UDC 575
DOI: 10.2298/GENSR1301217Z

Original scientific paper

A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION

Jana ŽIAROVSKÁ^1*, Eloy C. FERNÁNDEZ², Luigi MILLELA³

¹FAPZ, Slovak Agricultural University, Nitra, Slovakia.

² Faculty of Tropical AgriSciences, CULS – Prague, Czech Republic.

³Department of Science, University of Basilicata, Potenza Italy

Abstract

Žiarovská J., E. C. Fernández, and L. Millela (2013): A Revised its nucleotide sequence gives a specifity for Smallanthus sonchifolius (POEPP. and ENDL.) and its products identification. .Genetika, Vol 45, No. 1,217 -226.

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still known the paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and in all cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol was developed for yacon identification.

Key words: Smallantus sonchifolius (Poepp. and Endl.) H. Robinson, yacon, ITS, Dra III digestion, PCR

Corresponding author: Jana Žiarovska, FAPZ, Slovak Agricultural University, Nitra, Slovakia, email: jana.ziarovska@uniag.sk

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 227-236

Serbia

UDC 575
DOI: 10.2298/GENSR13012227G

Original scientific paper

GENETIC POLYMORPHISM OF GLUTATHION S-TRANSFERASE P1 (GSTP1) Ile105Val AND SUSCEPTIBILITY TO ATHEROGENESIS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS

Ivana GRUBIŠA ^1*, Petar OTAŠEVIĆ ^2,6 , Nebojša DESPOTOVIĆ ^3,6,

Velimir DEDIĆ ⁴ , Jelena MILAŠIN ⁵, Nada VUČINIĆ ¹

¹ Department of Human Genetics and Prenatal Diagnostics, Zvezdara University Medical Center, Belgrade, Serbia

²Dedinje Cardiovascular Institute , University of Belgrade, Belgrade, Serbia

³Geriatrics Department, Zvezdara University Medical Center, Belgrade, Serbia

⁴European Center for Peace and Development Established by United Nations University of Peace, Belgrade, Serbia;

⁵Institute of Human Genetics, School of Dentistry, University of Belgrade, Belgrade, Serbia

⁶ School of Medicine, University of Belgrade, Belgrade, Serbia

Abstract

Grubiša I., P. Otašević, N. Despotović, V. Dedić, J. Milašin and N. Vučinić (2013): Genetic polymorphism of glutathion S-transferase P1 (GSTP1) Ile105Val and susceptibility to atherogenesis in patients with type 2 diabetes mellitus. Genetika, Vol 45, No. 1, 227 -236.

One of the characteristics of type 2 diabetes mellitus (T2DM) is the state of persistent oxidative stress (OS) that has been implicated in the pathogenesis of diseases such is atherosclerosis mainly through chronic hyperglycemia that stimulates production of reactive oxygen species (ROS) and increases OS. Glutathione S-transferase P1 (GSTP1) is a member of the cytosolic GST superfamily. It plays an important role in neutralizing OS as an enzyme. Also, it participates in regulation of stress signaling and protects cells against apoptosis via its noncatalytic ligand-binding activity. GSTP1 Ile105Val functional polymorphism influences protein catalytic activity and stability and the aim of this study was to determine whether this gene variation influences susceptibility to atherogenesis in T2DM patients. A total of 240 individuals (140 patients with T2DM, accompanied with clinical manifestations of atherosclerosis, and 100 healthy controls) were included in this study. Genomic DNA was isolated from peripheral blood cells and genotyping was performed using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) analysis.

We obtained no statistically significant differences in the distribution of alleles and genotypes between cases and controls (P>0.05) but association between Ile/Val (OR=0.6, 95%CI=0.35-1.05, P=0.08) and Val/Val (OR=0.45, 95%CI=0.18-1.11, P=0.08) genotypes and disease approached significance (P=0.08). Our results indicated that a larger study group is needed to establish the true relationship between potentialiy protective allele Val and the disease, and to determine the influence of other GSTP1 polymorphisms on atherogenesis in T2DM patients.

Key words: GlutathioneS-transferase P1, gene polymorphism, type 2 diabetes mellitus, atherosclerosis

Corresponding author: Grubiša Ivana, Department of Human Genetics and Prenatal Diagnostics, Zvezdara University Medical Center, Preševska 31, Belgrade, Serbia, Tel: + 381 11 3810 600, Mail: i.pejin@lab.kbczvezdara.co.rs, ivanagrubisa@yahoo.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 237-249

Serbia

UDC 575
DOI: 10.2298/GENSR1301237G

Original scientific paper

SELECTION AND RAPD ANALYSIS of Pseudomonas ssp. ISOLATES ABLE TO IMPROVE BIOLOGICAL VIABILITY OF POTATO SEED TUBERS

Dobrivoj POŠTIĆ¹, Mira STAROVIĆ¹, Tatjana POPOVIĆ¹, Predrag BOSNIĆ, Aleksandra StanojkoviĆ- SEBIĆ², Radmila PiviĆ², Dragana JOŠIĆ²

¹Institute for Plant Protection and Environment, Belgrade,

²Institute of Soil Science, Belgrade

Abstract

Postic D., M. Starovic, T. Popovic, P. Bosnic, A. Stanojkovic-Sebic, R. Pivic, and D. Josic (2013): Selection and RAPD analysis of Pseudomonas ssp. isolates able to improve biological viability of potato seed tubers. Genetika, Vol 45, No. 1, 237-249.

Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 and Q16) and wild red clover (B25) were selected for the investigation of their effect on the biological vitality of the potato seed tubers. The production of IAA ranged from 4.09 to 15.9 µgmL^-1 after 24h of cultivation and 4.08 to 26.4 µgmL^-1after 48h of cultivation. The molecular comparison by RAPD analysis also was done. RAPD patterns of selected Pseudomonas spp. isolates obtained by BC318, AF14, SPH 1 and AP 10 primers demonstrated the suitability of RAPD method in distinguishing a high variability among the four isolates (44 to 68%). The effect on the biological viability of potato (industrially important variety Pirol) was observed during the seven weeks of sprouting at the temperature of 18-20°C. Potato tubers treated by the selected isolates formed slightly lower number of sprouts, but statistically higher mean length - up to 129.9% higher than the control. The mean sprouting capacity was 64.5% higher than a control. Obtained results suggested positive effects of selected IAA producing Pseudomonas isolates on the length of potato tubers and sprouting capacity as the parameters which define biological viability.

Key words: Pseudomonas spp., IAA, RAPD, biological viability, potato, tuber, sprout, sprouting capacity

Corresponding author: Dobrivoj Poštić, Institute for Plant Protection and Environment, T. Drajzera 9, 11040 Belgrade, Serbia, Tel: +381 11 2663-672; Fax: +381 11 2669-860; e-mail: pdobrivoj@yahoo.com

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 251-260

Serbia

UDC 575:630
DOI: 10.2298/GENSR1301251O

Original scientific paper

EFFECT OF ECOLOGICAL CONDITIONS ON EXPRESSION OF BIOPOMOLOGICAL CHARACTERISTICS OF CHESTNUT (Castanea sativa Mill.)

IN NATURAL POPULATIONS OF MONTENEGRO

Aleksandar ODALOVIĆ¹, Ranko PRENKIĆ¹,

Darko DUBAK¹, Miodrag JOVANČEVIĆ¹, Miroslav ČIZMOVIĆ¹, Marija RADUNOVIĆ²

¹Biotechnical Faculty, Podgorica, Montenegro

² Ministry of Agriculture and Rural Development, Montenegro

Abstract

Odalovic A., R. Prenkic, D.Dubak, M.Jovancevic, M.Cizmovic and M. Radunovic (2013): Effect of ecological conditions on expression of biopomological characteristics of chestnut (Castanea sativa mill.) in natural populations of Montenegro Genetika, Vol 45, No. 1, 251-260.

This paper presents the five-year long research results (2007-2011) of phenological observations (beginning, full flowering and the end of flowering), morphometric analysis (fruit weight, length, width and thickness of fruit, as well as length and width of the hilum), the ripening time and the average yield of selected genotypes of sweet chestnut (Castanea sativa Mill.). The results show that in terms of time of flowering chestnut trees examined in the group are very early flowering. The ripening of studied chestnut trees shows that the earliest harvest tree was Ostros II, VII and Kostajnica V (09.11.), and Kostajnica III (14.11.) were the latest. The average fruit weight of the selected sweet chestnut trees was (6.9 g). The highest average fruit weight (10.6 g), had the examined Ostros I tree, and the lowest (4.8 g), Kostajnica VI. The yield of the tested chestnut trees was on average (76.3 kg / tree). The selected trees Kostajnica II (66.0 kg / tree), Kostajnica I (69.0 kg / tree) had the smallest yield and Ostros VI (94.0 kg / tree) and Ostros V (87.0 kg / tree) had the highest. Obviously it can be concluded that population genetic variability is very high and gene expression is highly affected in tested samples on both locations. For further research it should be given attention to investigate genotypes in controlled conditions, the best in vitro in tissue culture.

Key words: Chestnut, chestnut tree, natural population, morphological and morphometric characteristics, yield.

Corresponding author: Aleksandar Odalović, Mihaila Lalića 1, Podgorica, Montenegro 81000, fax: 020 268 437, odalovica@t-com.me

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 261-272

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1301261K

Original scientific paper

THE DEPENDENCE OF MAIZE (Zea mays) HYBRIDS yielding potential ON THE WATER AMOUNTs REACHing THE SOIL SURFACE

Branka KRESOVIĆ ^1*, Vesna DRAGIĆEVIĆ¹, Boško Gajić²,

Angelina TAPANAROVA², Borivoj Pejić³

¹Maize Research Institute, Zemun Polje, Belgrade, Serbia

²Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

³Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

Abstract

Kresovic B., V. Dragicevic, B.Gajic, A.Tapanarova and B. Pejic(2013): The dependence of maize (Zea mays) hybrids yielding potential on the water amounts reaching the soil surface. Genetika, Vol 45, No. 1, 261-272.

The aim of the present study was to observe the response of maize hybrids under rainfed and irrigation conditions of the soil in order to establish the dependence of yielding potential on the water amounts reaching the soil surface during the growing season. The four-replicate trail was set up according to the randomised complete-block design on chernozem. Pre-watering soil moisture was approximately 70% of field water capacity, and soil moisture was established thermogravimetrically. During the five-year studies, the following differences in yields could be as follows: 12.68 t ha^-1 (ZP 341); 12.76 t ha^-1(ZP 434); 13.17 t ha^-1(ZP 578); 14.03 t ha^-1(ZP 684) and 13.75 t ha^-1(ZP 704) under conditions of 440 mm, 440 mm, 424 mm, 457 mm and 466 mm of water, respectively. The hybrid ZP 341, i.e. ZP 578 expressed the highest, i.e. the lowest tolerance in dry relative seasons, respectively. The reduction of the water amount for every 10 mm decreased the yield by 119.4 kg ha^-1 (ZP 341), 156.7 kg ha^-1 (ZP 434), 172.3 kg ha^-1 (ZP 578), 148.9 kg ha^-1 (ZP 684) and 151.1 kg ha^-1 (ZP 704).

Key words: genotype, maize, yield, rainfed conditions, irrigation

Corresponding author: Branka Kresović, Maize Research Institute “Zemun Polje”, Slobodana Bajica 1, 11185 Zemun Polje, Serbia, e-mail: bkresovic@mrizp.rs, Phone +381113756704, Fax: +381113756707

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Published in „GENETIKA“ Vol. 45, No. 1(2013), pp. 273-288

Serbia

UDC 575
DOI: 10.2298/GENSR1301273S
Review paper

THE Drosophila ananassae SPECIES COMPLEX: EVOLUTIONARY

RELATIONSHIPS AMONG DIFFERENT MEMBERS

B.N. SINGH and Seema SISODIA

Genetics Laboratory, Department of Zoology, Banaras Hindu University,

Varanasi-221 005, INDIA

Abstract

Singh B.N., and S. Sisodia (2013): The Drosophila ananassae species complex: evolutionary relationships among different members Genetika, Vol 45, No. 1, 273-288.

Information about genetic structure and historical demography of natural populations is central to understanding how natural selection changes genomes. Drosophila ananassae is a widespread species occurring in geographically isolated or partially isolated populations and provides a unique opportunity to investigate population structure and molecular variation. D. ananassae and its closely related species serve as a widely used model in population and evolutionary genetics. The ananassae subgroup belongs to the melanogaster species group. This subgroup contains 22 described species distributed mainly throughout Southeast Asia, with some species expanding into northeastern Australia, South Pacific and Indian subcontinent and Africa. Within the ananassae subgroup, three species complexes-ananassae, bipectinata and ercepeae have been recognized based on male genital morphology. D. ananassae and its relatives have many advantages as a model of genetic differentiation and speciation. In this review, distribution, phylogenies, hybridization, sexual isolation among D. ananassae complex have been discussed. The complex of several cryptic island species provides a useful model for evolutionary studies dealing with the mechanisms of speciation.

Key words: Drosophila ananassae species complex; phylogenetic relationships; different members

Corresponding author: B.N. Singh, Department of Zoology, Banaras Hindu University, Varanasi-221005, India. bnsingh@bhu.ac.in; bashisthsingh2004@rediffmail.com

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