GENETIKA, Vol. 45, No. 2 (2013)
Mihajlo ĆIRIĆ, Siniša JOCIĆ, Sandra CVEJIĆ, Milan JOCKOVIĆ, Petar ČANAK, Radovan MARINKOVIĆ, Mile IVANOVIĆ
COMBINING ABILITIES OF NEW INBRED LINES OF SUNFLOWER (Helianthus annuus l.) [Abstract] [Full text]
Danijela MILJKOVIĆ, Stevan AVRAMOV, Vukica VUJIĆ, Luka RUBINJONI, Nataša KLISARIĆ BARISIĆ, Uroš ŽIVKOVIĆ, Aleksej TARASJEV
BETWEEN-CLONE, BETWEEN-LEAF AND WITHIN-LEAF VARIATION IN LEAF EPIDERMIS TRAITS IN Iris pumila CLONES [Abstract] [Full text]
Barbara PIPAN, Jelka ŠUŠTAR-VOZLIČ, Vladimir MEGLIČ
GENETIC DIFFERENTIATION AMONG SEXUALLY
COMPATIBLE RELATIVES OF Brassica napus L. [Abstract]
[Full
text]
Sanja MATIĆ, Snežana STANIĆ, Slavica SOLUJIĆ, Nevena STANKOVIĆ, Milan MLADENOVIĆ and Vladimir MIHAILOVIĆ
Protective role of methanol extracts of Gentiana asclepiadea L. and G. cruciata L. against genotoxic damage induced by ethyl methanesulfonate [Abstract] [Full text]
Ana NIKOLIĆ, Violeta ANDJELKOVIĆ, Dejan DODIG, Snežana MLADENOVIĆ DRINIĆ, Natalija KRAVIĆ, Dragana IGNJATOVIĆ-MICIĆ
Identification of QTL-s for drought tolerance in maize, II : yield and yield components [Abstract] [Full text]
Ahmad Reza GOLPARVAR
GENETIC CONTROL AND COMBINING ABILITY OF FLAG
LEAF AREA AND RELATIVE WATER CONTENT TRAITS OF BREAD WHEAT CULTIVARS UNDER
DROUGHT STRESS CONDITION [Abstract] [Full
text]
Živko Ćurčić, Nevena Nagl, Ksenija Taški-Ajduković, Dario Danojević, željka STOJAKOVIĆ and Lazar Kovačev
Genetic diversity and
combining abilities FOR ROOT TRAITS of sugar beet pollinators [Abstract] [Full
text]
Mirjana SijAČiĆ-NikoliĆ, Јelena МilovanoviĆ , Мarina NoniĆ, Radmila KneŽeviĆ and Dragica StankoviĆ
LEAF
MORPHOMETRIC CHARACTERISTICS VARIABILITY OF DIFFERENT BEECH PROVENANCES IN
JUVENILE DEVELOPMENT STAGE [Abstract] [Full
text]
Can ERGÜN and Meral AKSOY
RELATIONSHIPS BETWEEN THE
HTAS2R38 GENOTYPE, FOOD CHOICE, AND ANTHROPOMETRIC VARIABLES IN NORMAL-WEIGHTED
AND OVERWEIGHT ADULTS [Abstract] [Full
text]
Ivana JOKSIĆ, Sandra PETROVIĆ, Andreja LESKOVAC, Jelena FILIPOVIĆ, Marija GUĆ-SĆEKIĆ, Dragana VUJIĆ and Gordana JOKSIĆ
ENHANCED FREQUENCY OF SISTER CHROMATID
EXCHANGES INDUCED BY DIEPOXYBUTANE IS SPECIFIC CHARACTERISTIC OF FANCONI
ANEMIA CELLULAR PHENOTYPE [Abstract] [Full
text]
Dragana IgnjatoviĆ-MiciĆ, Danijela RistiĆ, Vojka BabiĆ, Violeta AndjelkoviĆ, Ksenija MARKOVIĆ and Jelena VanČetoviĆ
Genetic assessment of maize
landraces from former Yugoslavia [Abstract] [Full text]
Vlado KOVAČEVIĆ, Domagoj ŠIMIĆ, Zvonimir ZDUNIĆ and Zdenko LONČARIĆ
GENOTYPE AND LIMING IMPACTS ON BORON AND MOLYBDENUM STATUS IN MAIZE [Abstract] [Full text]
Emina Mladenović, Janoš Berenji, Ksenija Hiel, Marija Kraljević-Balalić, Vladislav Ognjanov, Mirjana Ljubojević and Jelena Čukanović
Inheritance of warty fruit texture and fruit color in bottle gourd [Lagenaria siceraria (Molina) Standl.] [Abstract] [Full text]
Darko GRUJIČIĆ, Slobodan ARSENIJEVIĆ, Dragoslav MARINKOVIĆ, Olivera MILOŠEVIĆ-DJORDJEVIĆ
INTER-INDIVIDUAL VARIANCE IN MICRONUCLEI FREQUENCY IN LYMPHOCYTES OF PATIENTS WITH CERVICAL INSUFFICIENCY [Abstract] [Full text]
Abdollah GHASEMI PIRBALOUTI, Mehdi BARANI, Behzad HAMEDI, Mehrdad ATAEI KACHOUEI, and Abnoos KARIMI
ENVIRONMENT EFFECT ON DIVERSITY
IN QUALITY AND QUANTITY OF ESSENTIAL OIL OF DIFFERENT WILD POPULATIONS OF
KERMAN THYME [Abstract] [Full
text]
Sanja ĆIRKOVIĆ, Tatjana SRDIĆ-RAJIĆ, Marija GUĆ-ŠĆEKIĆ, Dragana VUJIĆ , Dragan MIĆIĆ and Dejan ŠKORIĆ
CELL CYCLE ANALYSIS IN PATIENTS WITH FANCONI ANEMIA FROM SERBIA [Abstract] [Full text]
Velimir RADIĆ, Jelena MRĐA, Sreten TERZIĆ, Boško DEDIĆ, Aleksandra DIMITRIJEVIĆ, Igor BALALIĆ, Dragana MILADINOVIĆ
CORRELATIONS AND PATH ANALYSES OF YIELD AND OTHER SUNFLOWER SEED CHARACTERS [Abstract] [Full text]
Fuad GAŠI, Maja ŽULJ MIHALJEVIĆ, Silvio ŠIMON, Jasmin GRAHIĆ, Naris POJSKIĆ,Mirsad KURTOVIĆ, Dragan NIKOLIĆ, Ivan PEJIĆ
GENETIC STRUCTURE OF APPLE ACCESSIONS MAINTAINED EX SITU IN BOSNIA AND HERZEGOVINA EXAMINED BY MICROSATELLITE MARKERS [Abstract] [Full text]
Katarina VELJOVIĆ, Amarela TERZIĆ-VIDOJEVIĆ, Maja TOLINAČKI, Milan KOJIĆ and Ljubiša TOPISIROVIĆ
MOLECULAR ANALYSIS OF ENTEROLYSIN A AND ENTL GENE CLUSTER FROM NATURAL ISOLATE Enterococcus faecalis BGPT1-10P [Abstract] [Full text]
Dragan KOVAČEVIĆ, Biljana NIKOLIĆ, Snežana MLADENOVIĆ DRINIĆ, Srđan BOJOVIĆ, Tanja DODOŠ, Nemanja RAJČEVIĆ, and Petar D. MARIN
GENETIC RELATIONSHIPS AMONG SOME Pinus, Picea AND Abies SPECIES REVEALED BY RAPD MARKERS [Abstract] [Full text]
Jalal GHARESOURAN, Maryam REZAZADEH, Seiied MOJTABA MOHADDES ARDEBILI
INVESTIGATION OF FIVE POLYMORPHIC DNA MARKERS ASSOCIATED WITH LATE ONSET ALZHEIMER DISEASE [Abstract] [Full text]
Mihajla DJAN, NevenaVELIČKOVIĆ, Dragana OBREHT, Nataša KOČIŠ TUBIĆ, Vladimir MARKOVIĆ, Milan STEVANOVIĆ, Miloš BEUKOVIĆ
MITOCHONDRIAL DNA CONTROL REGION VARIABILITY IN WILD BOARS FROM WEST BALKANS [Abstract] [Full text]
N.B. SINGH, S. JOSHI, P. CHOUDHARY & J P SHARMA
SSR DNA MARKER AIDED GENETIC DIVERSITY
ASSESSMENT OF SELECTED WILLOW CLONES [Abstract] [Full
text]
Marija SAVIĆ VESELINOVIĆ1, Sofija PAVKOVIĆ-LUČIĆ1, Zorana KURBALIJA NOVIČIĆ2, Mihailo JELIĆ1, Marko ANĐELKOVIĆ1,2,3
SEXUAL SELECTION CAN REDUCE MUTATIONAL LOAD IN Drosophila subobscura [Abstract] [Full text]
Zeljko LAKIC, Dejan SOKOLOVIC, Snezana BABIC, Svetko VOJIN, Jela IKANOVIC, Tatjana Veljovic, Igor BALALIC
GENETIC VARIABILITY OF SEED YIELD AND SEED YIELD COMPONENTS OF AUTOCHTHONOUS Lolium perenne L. POPULATIONS [Abstract] [Full text]
Jasna SAVIĆ, Ana MARJANOVIĆ-JEROMELA, Đorđe GLAMOČLIJA, Slaven PRODANOVIĆ
OILSEED RAPE GENOTYPES RESPONSE TO BORON
TOXICITY [Abstract] [Full
text]
Eva MACHANSKÁ, Vladimír BAJCAR, Roman LONGAUER, Dušan GÖMÖRY
EFFECTIVE POPULATION SIZE ESTIMATION IN
SEED ORCHARDS: A CASE STUDY OF Pinus nigra ARNOLD AND Fraxinus excelsior L./ F. angustifoliA
VAHL. [Abstract] [Full
text]
Vojka BABIĆ, Slaven PRODANOVIĆ, Milosav BABIĆ, Nenad DELIĆ, Violeta ANĐELKOVIĆ
The identification
of bands related to yields and stability in maize hybrids and their parental
components [Abstract] [Full
text]
Velichka TODOROVA, Stanislava GROZEVA, Velichka RODEVA, Stoyka MASHEVA
BREEDING EVALUATION OF PEPPER LINES OBTAINED BY IN VITRO ANTHER CULTURE [Abstract] [Full
text]
Oindrila RAHA, B.N.SARKAR, P.VEERRAJU, G. SUDHAKAR,
P.RAYCHAUDHURI, Soma MUKHOPADHYAY, V.R.RAO
ROLE OF MICA REPEAT
POLYMORPHISM IN THE MANIFESTATION OF TYPE 1 Diabetes mellitus IN BENGALI
INDIAN PATIENTS [Abstract] [Full
text]
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 289-296
© 2013 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.8
DOI: 10.2298/GENSR1302289C
Original scientific paper
COMBINING ABILITIES OF NEW INBRED LINES OF SUNFLOWER
(Helianthus annuus L.)
Mihajlo ĆIRIĆ, Siniša JOCIĆ, Sandra CVEJIĆ, Milan JOCKOVIĆ, Petar ČANAK,
Radovan MARINKOVIĆ, Mile IVANOVIĆ
Institut of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Ćirić M., S. Jocić, S. Cvejić, M. Jocković, P. Čanak, R.Marinković and M.Ivanović (2012): Combining abilities of new inbred lines of sunflower (Helianthus annuus L.)- Genetika, vol., No.45, No.2, 289-296.
Combining abilities for plant height and head diameter of ten sterile A-lines, three restorers - RF lines and their (10x3) F1-hybrids were examined by line x tester method. Significant differences were found between A-lines, RF-lines (testers) and their hybrids. The highest mean for plant height had line MI-A-57 (198.17 cm) and hybrid combination MG-MI-1 x MI-A-57 (239 cm), and the lowest mean had line PL-DI-13 (79.67 cm) and hybrid MG-MI-4 x PL-DI-13 (152.67 cm). Line PL-DI-52 (24.47 cm) and hybrid MG-MI-4 x MI-A-57 (25.37 cm) had the highest mean for head diameter, and the lowest mean was determined in line PL-DI-44 (13.60 cm) and hybrid MG-MI-2 x PL-DI-15 (17.90 cm). Lines with the best general combining abilities were PL-DI-13 for plant height and MI-A-57 for head diameter. Hybrids with the best specific combining abilities were MG-MI-2 x PL-DI-15 for plant height and MG-MI-1 x PL-DI-15 for head diameter.
Key words: sunflower, combining abilities, General Combining Ability, Specific Combining Ability, plant height, head diameter
Corresponding author: Mihajlo Ćirić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 NoviSad, e-mail: mihajlo.ciric@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 297 -308
© 2013 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575
DOI:10.2298/GENSR1302297M
Original scientific paper
BETWEEN-CLONE, BETWEEN-LEAF AND WITHIN-LEAF VARIATION IN LEAF EPIDERMIS TRAITS IN Iris pumila CLONES
Danijela MILJKOVIĆ 1*, Stevan AVRAMOV 1, Vukica VUJIĆ 2, Luka RUBINJONI 2, Nataša KLISARIĆ BARISIĆ 1, Uroš ŽIVKOVIĆ 1, Aleksej TARASJEV 1
1Institute for Biological Research “S. Stanković“,University of Belgrade, Belgrade, Serbia
2 Faculty of Biology, University of Belgrade, Belgrade, Serbia
Abstract
Miljković D., S. Avramov, V. Vujić, L. Rubinjoni, N. Klisarić Barisić, U. Živković and A. Tarasjev (2013): Between-clone, between-leaf and within-leaf variation in leaf epidermis traits in Iris pumila clones.Genetika, Vol 45, No. 2, 297-308.
The goal of this study was to analyze variation and covariation in epidermal characteristics (epidermal cell density -ECD, stomata density - SD, and stomata index -SI) on Iris pumila clones on between-clone, between-leaf and within-leaf levels. ECD (similar to the pattern previously observed for SD) increased from the base to the top of leaf, while SI remained constant. Results of profile analyses indicated that clones, individual plants whitin clones (ramets), and three successive leaves on the same plant were not significantly different for examined characteristics, but genetic variation for position effect was detected (significant Zone x clone interaction). Results of the contrast analysis confirmed differences between the base and middle leaf positions for ECD (similar to those for SD) as well as between clone variation for those differences. Observed differences between leaf zones and correlations between analyzed traits were mostly consistent with the expansion hypothesis of stomata differentiation.
Key words: epidermal traits, stomatal density, epidermal cell density, stomata index, Iris pumila.
Corresponding author: Miljković Danijela, Department of Evolutionary Biology,Institute for Biological Research, “S. Stanković“University of Belgrade,Despota Stefana 142,11000 Belgrade, Serbia,phone: +381 11 207 83 76, Fax: +381 11 761 433,Mobtel.:+381 64 24 17 228,e-mail: danijela.miljkovic@ibiss.bg.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 309- 327
© 2013 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
DOI:
10.2298/GENSR1302309P
Original scientific paper
GENETIC DIFFERENTIATION AMONG SEXUALLY COMPATIBLE RELATIVES OF Brassica napus L.
Barbara PIPAN, Jelka ŠUŠTAR-VOZLIČ, Vladimir MEGLIČ
Agricultural Institute of Slovenia, Crop Science Department, Ljubljana, Slovenia
Abstract
Pipan B., J. Šuštar-Vozlič, and V. Meglič (2013): Genetic differentiation among sexually compatible relatives of Brassica napus L..Genetika, Vol 45, No. 2, 309-327.
Analysis of gene flow between Brassica napus L. and its sexually compatible relatives that could be found in the wild in Slovenia was performed by microsatellite analysis using fifteen selected primer pairs. Genotypes included in the study were obtained from the field survey of sexually compatible relatives of B. napus in natural habitats around Slovenia and from reference collections. Two different wild species of all the presented sexually compatible relatives of B. napus were found in Slovenia, B. rapa and Sinapis arvensis. The reference genotypes included varieties and wild forms from internal collections as marketable seeds or from gene banks. Reference genotypes were represented by the following species and subspecies: B. napus ssp. napobrassica, B. napus ssp. napus, B. nigra, B. oleracea, B. rapa ssp. oleifera, Diplotaxis muralis; D. tenuifolia, Raphanus raphanistrum, R. sativus, R. sativus var. oleiformis, Rapistrum rugosum, S. alba and S. arvensis. Estimation of gene flow described by average number of migrants was 0.72 followed by 0.20 migrants. Due to the observed gene migrations, genetic drift and selection, Hardy-Weinberg equilibrium was not met. The mean number of alleles over all loci was 16.9, the average polymorphic information content was 0.43. We found four highly divergent and polymorphic loci (Na12-C08, Na10-A08, Ni3-G04b and BRMS-050) at statistically significant level (p<0.05) of gene flow detected. Over all gene diversity intra-individual among populations (0.55) was lower than inter-individual among population (0.77). The results of genetic linkages based standard genetic distance and unweighted pair group method with arithmetic mean clustering method, generally divided the genotypes in three divergent groups. Similar results were obtained by principal coordinate analysis where three main groups were constructed according to three factors. A real number of genetic clusters demonstrated a clear separation between populations, where only one was comprised from genotypes from other origins. The 30.7 % out-crossing rate of B. rapa and S. arvensis populations from filed survey represents the potential for spontaneous inter-and intra-specific gene flow under Slovenian production area.
Key words: Brassica napus L./ sexually compatible relatives/ microsatellites/ gene flow/ genetic diversity/ out-crossing rate
Corresponding author: Vladimir Meglič, Agricultural Institute of Slovenia, Crop Science Department, Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia, Tel.: +386 1 2805 180; fax: +386 1 2805 255. E-mail address: vladimir.meglic@kis.si
Published in „GENETIKA“ Vol. 45, No.2 (2013), pp. 329 -340
© 2013Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302329M
Original scientific paper
Protective role of methanol extracts of Gentiana asclepiadea L. and G. cruciata L. against genotoxic damage induced by ethyl methanesulfonate
Sanja MATIĆ a,*, Snežana STANIĆ a, Slavica SOLUJIĆ b, Nevena STANKOVIĆb,
Milan MLADENOVIĆ b and Vladimir MIHAILOVIĆ b
aDepartment of Biology and Ecology, Faculty of Science, University of Kragujevac, Serbia
bDepartment of Chemistry, Faculty of Science, University of Kragujevac, Serbia
Abstract
Matić S., S. Stanić, S. Solujić, N. Stanković, M. Mladenović and V. Mihailović (2013): Protective role of methanol extracts of Gentiana asclepiadea L. and G. cruciata L. against genotoxic damage induced by ethyl methanesulfonate. Genetika, Vol 45, No. 2, 329-340.
The methanol extracts from the underground and aerial part of the two species of Gentiana genus, Gentiana asclepiadea L. and Gentiana cruciata L. from Serbia, were investigated for their antigenotoxic activity against well-established mutagenic agent ethyl methanesulfonate (EMS) using the in vivo sex-linked recessive lethal (SLRL) test on Drosophila melanogaster. For this purpose, three days old Canton S males were treated with the potent mutagen EMS in concentration of 0.75 ppm, alone and combined with methanol extracts obtained from underground or aerial part of G. asclepiadea and G. cruciata in concentration of 5%, separately. Although EMS in concentration of 0.75 ppm increased the mutation frequency in all three broods, post-treatments with methanol extracts obtained from the underground and aerial part of G. asclepiadea and G. cruciata in concentration of 5%, respectively, drastically reduced the frequency of sex-linked recessive lethal mutations induced by EMS. Compared to the sucrose, as a negative control, methanol extract obtained from underground part of G. cruciata showed the most potent antigenotoxic activity. Extracts from the underground and aerial part of the two species of Gentiana genus, G. asclepiadea L. and G. cruciata L. from Serbia used in our experiments showed a clear antimutagenic effect, reducing the frequency of mutations induced by a strong mutagen such as EMS.
Key words: antigenotoxic, methanol extracts, Gentiana asclepiadea, Gentiana cruciata, SLRL test
Corresponding author: Sanja Matić, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, Kragujevac, Serbia.Tel: (+381) 34 336 223, Fax: (+381) 34 335.040
e-mail:sanjamatic@kg.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 341 -350
© 2013 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
DOI:
10.2298/GENSR1302341N
Original scientific paper
Identification of QTL-s for drought tolerance in maize, II : yield and yield components
Ana NIKOLIĆ, Violeta ANDJELKOVIĆ, Dejan DODIG, Snežana MLADENOVIĆ DRINIĆ, Natalija KRAVIĆ, Dragana IGNJATOVIĆ-MICIĆ
Maize Research Institute „ZemunPolje“, Belgrade, Serbia
Abstract
Nikolić A., V. Anđelković, D. Dodig, S. Mladenović Drinić, N.Kravić and D. Ignjatović Micić (2013): Identification of Qtl-s for drought tolerance in maize, II : Yield and yield components. Genetika, Vol 45, No. 2, 341-350.
Grain yield is the primary trait of interest in maize breeding programs. Worldwide, drought is the most pervasive limitation to the achievement of yield potential in maize. Drought tolerance of maize has been considerably improved through conventional breeding. Traditional breeding methods have numerous limitations, so development of new molecular genetics techniques could help in elucidation of genetic basis of drought tolerance .In order to map QTLs underlying yield and yield components under drought 116 F3 families of DTP79xB73 cross were evaluated in the field trials. Phenotypic correlations calculated using Pearson’s coefficients were high and significant. QTL detection was performed using composite interval mapping option in WinQTL Cartographer v 2.5. Over all nine traits 45 QTLs were detected:five for grain yield per plant and 40 for eight yield components. These QTLs were distributed on all chromosomes except on chromosome 9. Percent of phenotypic variability determined for the identified QTLs for all the traits was in the range from 27.46 to 95.85%. Different types of gene action were found for the QTLs identified for analyzed traits.
Key words: drought tolerance, maize, QTLs, yield, yield components
Corresponding author: Ana Nikolić, Maize Research Institute „ZemunPolje“,S. Bajića 1, 11185 Belgrade, Serbia,e-mail: anikolic@mrizp.rs
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 351 -360
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
DOI: 10.2298/GENSR1302351G
Original scientific paper
GENETIC CONTROL AND COMBINING ABILITY OF FLAG LEAF AREA AND RELATIVE WATER CONTENT TRAITS OF BREAD WHEAT CULTIVARS UNDER DROUGHT STRESS CONDITION
Ahmad Reza GOLPARVAR
Department of Agronomy and Plant Breeding, Khorasgan (Isfahan) Branch, Islamic Azad University, Isfahan, Iran
Abstract
Golparvar R.A. (2013): Genetic control and combining ability of flag leaf area and relative water content traits of bread wheat cultivars under drought stress condition. Genetika, Vol 45, No. 2, 351-360.
In order to compare mode of inheritance, combining ability, heterosis and gene action in genetic control of traits flag leaf area, relative water content and grain filling rate of bread wheat under drought stress, a study was conducted on 8 cultivars using of Griffing’s method2 in fixed model. Mean square of general combining ability was significant also for all traits and mean square of specific combining ability was significant also for all traits except relative water content of leaf which show importance of both additive and dominant effects of genes in heredity of these traits under stress. GCA to SCA mean square ratio was significant for none of traits. Results of this study showed that non additive effects of genes were more important than additive effect for all traits. According to results we can understand that genetic improvement of mentioned traits will have low genetic efficiency by selection from the best crosses of early generations. Then it is better to delay selection until advanced generations and increase in heritability of these traits.
Key words: Diallel analysis, bread wheat, drought stress, genetic improvement, general and specific combining ability
Corresponding author: Ahmad Reza Golparvar,Department of Agronomy and Plant Breeding, Khorasgan (Isfahan) Branch, Islamic Azad University, P.O.Box:81595-158, Isfahan, Iran, e-mail:dragolparvar@gmail.com
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 361 -368
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.63
DOI:
10.2298/GENSR1302361C
Original scientific paper
Genetic diversity and combining abilities FOR ROOT TRAITS of sugar beet pollinators
Živko Ćurčić, Nevena Nagl, Ksenija Taški-Ajduković, Dario Danojević,
željka STOJAKOVIĆ and Lazar Kovačev
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Ćurčić Ž., N. Nagl, K. Taški-Ajduković, D. Danojević, Ž. Stojaković and L. Kovačev (2013): Genetic diversity and combining abilities for root traits of sugar beet pollinators. Genetika, Vol 45, No. 2, 361-368.
Information about genetic diversity and combining abilities of sugar beet parental components are of a great importance for hybrid creation. The aim of this research was to evaluate genetic diversity among sugar beet pollinators from different breeding programs and their combining abilities for main root traits of sugar beet, root weight, sugar content and sugar yield. As plant material were used eight pollinators originating from three different USDA-ARS breeding programs and four from Institute of field and vegetable crops Novi Sad. The analysis of variance and Duncan’s multiple range test revealed significant differences (p = 0.05) among pollinators for all investigated traits. Pollinator CR10 differs from all others in terms of quantitative traits and in terms of combining ability. Despite small root weight CR10 had significantly positive GCA for that trait and showed that genotypes with small root should not be automatically discarded. Pollinators from Institute of field and vegetable crops used in this research had negative GCA for root weight and should be used only as hosts for introduction of new germplasm in future breeding program.
Key words: genetic diversity, sugar beet, pollinator, breeding
Corresponding author: Živko Ćurčić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad,Phone 021 4898 328, Fax 021 6621 212,E-mail zivko.curcic@nsseme.com
Published in „GENETIKA“ Vol. 45, No.2 (2013), pp. 369 -380
© 2010 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
DOI:
:10.2298/GENSR1302369N
Original scientific paper
LEAF MORPHOMETRIC CHARACTERISTICS VARIABILITY OF DIFFERENT BEECH PROVENANCES IN JUVENILE DEVELOPMENT STAGE
Mirjana SijAČiĆ-NikoliĆ1, Јelena МilovanoviĆ2 , Мarina NoniĆ1,
Radmila KneŽeviĆ1 and Dragica StankoviĆ1
1 Faculty of Forestry, University of Belgrade, Belgrade, Serbia
2University “Singidunum“, Faculty of Applied Ecology “Futura“,Belgrade, Serbia
Abstract
Šijačić Nikolić M., J. Milovanović, M. Nonić, R. Knežević, and D. Stanković (2013): Leaf morphometric characteristics variability of different beech provenances in juvenile development stage.Genetika, Vol 45, No. 2, 369-380.
The taxonomic status of beech from the Balkan Peninsula is not yet clearly defined. There is no agreement among different authors about the morphological characteristics discriminating between the Balkan and European and/or Eastern beech. For most characteristics, the mean values are different but the ranges of variation overlap considerably. Provenance trial of beech established in Serbia, at the locality Debeli Lug, has provided an opportunity for research of inter-provenance variability at the level of leaf morphometric characteristics in juvenile development stage. Research included 10 provenances originating from the Western Balkans (Serbian provenance 36 and 38; Croatian provenance 24 and 25; Bosnian provenance 30 and 32) and from Central Europe (German provenance 47 and 49; Austrian provenance 56 and Hungarian provenance 42), where following morphometric characteristics were analyzed: leaf length (Ll), leaf width (Lw), petiole lenght (Pl), leaf base width on 1 cm (Blw), number of veins – left (Vl), number of veins – right (Vr), distance between 3rd and 4th vein – left (Dv 3-4). The results of this research show existence of clear differentiation among provenances from the Western Balkan and from Central Europe, from the point of leaf dimensions, number of veins and leaf base width.
Key words: Balkan beech, variability, leaves, juvenile development stage
Corresponding author: Marina Nonić, Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade, Serbia, Phone: +381 11 3053 873; E-mail: marina.nonic@sfb.bg.ac.rs
Published in „GENETIKA“ Vol. 45, No.2 (2013), pp. 381-391
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302381E
Original scientific paper
RELATIONSHIPS BETWEEN THE HTAS2R38 GENOTYPE, FOOD CHOICE, AND ANTHROPOMETRIC VARIABLES IN NORMAL-WEIGHTED AND OVERWEIGHT ADULTS
Can ERGÜN a and Meral AKSOY b
a Bahçeşehir University, Faculty of Health Sciences Department of Nutrition and Dietetics, Beşiktaş, Istanbul,Turkey
b Hacettepe University Faculty of Health Sciences Department of Nutrition and Dietetics, Sıhhiye, Ankara,Turkey
Abstract
Ergun C, and M. Askoy (2013): Relationships between the htas2r38 genotype, food choice, and anthropometric variables in normal-weighted and overweight adults. Genetika, Vol 45, No. 2, 381-391.
Aim: Taste is a major determinant of food choice; however, there is a great lack of knowledge about how taste perception affects human nutrition. Bitter taste perception presents unique opportunities for investigating this subject. The aim of this study was to determine whether polymorphisms on the bitter taste receptor gene hTAS2R38 affect an individual’s food choices and some anthropometric variables. Subjects and Method: In this study, the possible relationship between food preferences, body weight, and polymorphisms on hTAS2R38 was investigated in healthy volunteers (n=178) who weighed within the normal range (BMI: 20-24.9 kg/m2, n=90) and those who were overweight, but otherwise healthy (BMI≥25.0 kg/m2, n=88). Descriptive information about the subjects was collected via a questionnaire, and anthropometric measurements were taken by the researcher. Records of three consecutive days of food consumption were collected to determine each subject’s macronutrient intake. For identification of the hTAS2R38 genotype, samples were taken from each participant's in-mouth epithelial cell line, and the genetic material was analyzed at the laboratory for Rs713598. Results: The percentage of “non-tasters” (n=42) among the whole population was 23.6% (C-Homozygote: 23.6%) while “tasters” (n=136) comprised 76.4% (CG-Heterozygote: 46.6%, G-Homozygote: 29.8%). When group-wide and between-group comparisons were made, it was revealed that taster status didn’t affect differences in anthropometric measures. Detected differences in macronutrient intake were due to gender. Discussion: Polymorphisms on hTAS2R38 bitter taste receptor gene had no effect on variables such as body weight, anthropometric variables, body fat percentage, or food choices within the study population.
Key words: Single Nucleotide Polymorphism, TAS2R38 protein, Food preferences, Body weight and measurements
Corresponding author: Can Ergün, Bahçeşehir University, Faculty of Health Sciences Department of Nutrition and Dietetics, Beşiktaş, Istanbul-TURKEY, Tel: +90-212-3815408, Fax: +90-212-3810027, e-mail: can.ergun@bahcesehir.edu.tr
Published in „GENETIKA“ Vol. 45, No. 2 (2013), pp. 393 - 403
© 2013 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
ENHANCED FREQUENCY OF SISTER CHROMATID EXCHANGES INDUCED BY DIEPOXYBUTANE IS SPECIFIC CHARACTERISTIC OF FANCONI ANEMIA CELLULAR PHENOTYPE
Ivana JOKSIĆ1, Sandra PETROVIĆ1, Andreja LESKOVAC1, Jelena FILIPOVIĆ1, Marija GUĆ-SĆEKIĆ2, 3, Dragana VUJIĆ2, 4 and Gordana JOKSIĆ1*
1Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia
2Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Belgrade, Serbia
3Faculty of Biology, University of Belgrade, Belgrade, Serbia
4School of Medicine, University of Belgrade, Belgrade, Serbia
Abstract
Joksić I, S. Petrović, A. Leskovac, J Filipović, M.Guč-Šćekić, D.Vujić and G. Joksić (2013): Enhanced frequency of sister chromatid exchanges induced by diepoxybutane is specific characteristic of Fanconi anemia cellular phenotype.Genetika, Vol 45, No. 2, 393-403.
Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in whole-blood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The base-line frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.
Key words: diepoxybutane, Fanconi anemia lymphocytes, ionizing radiation, sister chromatid exchange
Corresponding author: Dr. Gordana Joksić, Department of physical chemistry, Vinca Institute of Nuclear Sciences, M. Petrovica Alasa 12-14, 11001 Belgrade, Serbia,Tel. +381113408565,Fax +381118066434,E-mail: gjoksic@vinca.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 405 - 417
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
DOI:
10.2298/GENSR1302405I
Original scientific paper
Genetic assessment of maize landraces from former Yugoslavia
Dragana IgnjatoviĆ-MiciĆ, Danijela RistiĆ, Vojka BabiĆ, Violeta AndjelkoviĆ, Ksenija MARKOVIĆ and Jelena VanČetoviĆ
Maize Research Institute Zemun Polje, Belgrade, Serbia
Abstract
Ignjatović Micić D., D. Risitć, V Babić, V. Anđelković, K.Marković and J. Vančetović (2013): Genetic assessment of maize landraces from former Yugoslavia. Genetika, Vol 45, No. 2, 405-417.
A collection of 2217 landraces from former Yugoslavia region is maintained at Maize Research Institute Zemun Polje gene bank. All local varieties from the former Yugoslavia are classified into 18 groups. These agro-ecological groups encompass early introduced flint types grown on small and isolated areas, later introduced dent types that spread on wide areas of crop production and types created through hybridization between these two kernel types. The objective of this research was to study population structure, genetic diversity and relationships of nine flint and nine dent accessions belonging to different agro-ecological groups using phenotypic and simple sequence repeat (SSR) markers. The 18 analyzed landraces displayed great variation for most analyzed traits, but flint landraces were more diverse. Ten SSR probes revealed total of 56 and 62 alleles in flint and dent landraces, respectively. Eight specific alleles (i.e. alleles found only in one landrace or only within flint, i.e. dent landraces) were detected with five probes. One specific allele was found in flint and seven alleles in dent landraces. These differences in allele structure point to different origins and possibly different purposes of flint and dent genotypes. Both phenotypic and SSR analyses could distinguish most flint and dent landraces, but not agro-ecological groups. The results revealed a significant genetic heterogeneity indicating that the analyzed landraces could be valuable sources of genetic variability.
Key words: genetic diversity, landraces, maize, phenotype, SSR
Corresponding author: Dragana Ignjatovic-Micic, S. Bajica1,11185 Belgrade.Serbia, Phone: 3756704,Fax: 3756707,e-mail: idragana@mrizp.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 419 -426
© 2013Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
DOI:
10.2298/GENSR1302419K
Original scientific paper
GENOTYPE AND LIMING IMPACTS ON BORON AND MOLYBDENUM
STATUS IN MAIZE
Vlado KOVAČEVIĆ1, Domagoj ŠIMIĆ2, Zvonimir ZDUNIĆ2 and Zdenko LONČARIĆ1
1 University J.J. Strossmayer in Osijek, Faculty of Agriculture, Osijek, Croatia;
2 Agricultural Institute, Osijek, Croatia
Abstract
Kovačević V., D. Šimić, Z. Zdunić, and Z. Lončarić (2013):Genotype and liming impacts on boron and molybdenum status in maize Genetika, Vol 45, No. 2, 419-427.
Liming experiment with hydrated lime (73% CaO + 2-3% MgO + 21% water) in the amounts 0, 5 and 20 t ha-1 was conducted in spring 2006. Six domestic maize hybrids (B1 = Os298P, B2 = Tvrtko303, B3 = Os444, B4 = Os499, B5 = Os552 and B6 = Os596) were sown at beginning of May (basic plot 24 m2). The ear-leaf of maize was collected at flowering and grain in maturity of the 2006 and 2007 growing seasons. Boron and molybdenum in the samples were determined by the ICP-OES method. The growing season 2006 was favorable for maize growth, while 2007 characterized stress due to drought and high air-temperatures. B and Mo concentrations (2-yr means) in grains were considerably lower for 12-fold (B) and close to two-fold (Mo) in comparison with the leaves. Leaf-Mo in 2007 was about 50% lower in comparison with 2006 (0.310 and 0.149 mg Mo kg-1, respectively). Mean differences among the hybrids (mg kg-1) were as follows: leaf-B 9.3 (B1) to 21.4 (B3) and grain-B 1.00 (B2) to 1.50 (B3); leaf-Mo 0.157 (B5) to 0.361 (B3) and grain-Mo 0.109 (B5) to 0.137 (B1). As affected by liming leaf-Mo were increased compared with the control 2.5-fold and 4.1 fold, grain-Mo 3.8-fold and 4.6-fold, for 5 and 20 t ha-1 lime, respectively. Liming effects on B concentrations in maize were considerably lower, because non-significant differences for leaf-B in 2006 and decrease for 36% in 2007 were found.
Key words: boron, molybdenum, grain, leaf, liming, maize hybrids
Corresponding author: Vlado Kovačević, Faculty of Agriculture, Kralja P. Svačića 1d,31000 Osijek, Croatia; Phone: ++385 31 554 932, e-mail: vkovacevic@pfos.hr
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 427-432
© 2013Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.1
DOI:
10.2298/GENSR1302427М
Original scientific paper
Inheritance of warty fruit texture and fruit color in bottle gourd [Lagenaria siceraria (Molina) Standl.]
Emina Mladenović1, Janoš Berenji2, Ksenija Hiel1, Marija Kraljević-Balalić1, Vladislav Ognjanov1, Mirjana Ljubojević1 and Jelena Čukanović1
1 Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia
2 Institute for field and crops, Novi Sad, Serbia
Abstract
Mladenović E., J. Berenji, K. Hiel, M.Kraljević Balalić, V.Ognjanov, M.Ljubojević and J. Čukanović (2013): Inheritance of warty fruit texture and fruit color in bottle gourd [Lagenaria siceraria (molina) standl.]. Genetika, Vol 45, No. 2, 427-432.
Bottle gourd [Lagenaria siceraria (Molina) Standl.] is one of the most interesting species in the plant kingdom, due to the diversity of fruit shapes, sizes and ways of use. Warty genotypes are rare compared to non warty genotypes. Considering unusual external appearance of warty fruits, we focused our research on the investigation of its inheritance patterns. By crossing different bottle gourd phenotypes, we studied the mode of inheritance and identified and verified genes responsible for the fruit skin color and warty phenotype segregation. Two parental lines, LAG 70 (with warty fruit of light green color) and LAG 71 (smooth fruit, variegated), F1, F2 and backcrosses populations along with both parents were evaluated. Genetic analysis indicated that warty fruit type is a result of monogenic inheritance, whereby the warty fruit type is dominant (Wt) trait over to the non-warty fruit type (wt). The mode of inheritance of fruit color was controlled by recessive epistasis, with a ratio of 9 variegated (A-, B-), 3 dark green colored (aaB-) and 4 light green colored (aabb) fruits in the F2 generation.
Key words: bottle gourd, fruit color, inheritance, warty fruits
Corresponding author: Emina Mladenović, University of Novi Sad, Faculty of Agriculture, Department of fruit science, viticulture, horticulture and landscape arhitecture, Dositeja Obradovića Square 30, 21000 Novi Sad, tel: +38121 485 3269, fax:+381 21 450 123, e-mail: eminam@polj.uns.ac.rs
Published in „GENETIKA“ Vol. 45, No.2(2013), pp. 433 - 439
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI: 10.2298/GENSR1302433G
Original scientific paper
INTER-INDIVIDUAL VARIANCE IN MICRONUCLEI FREQUENCY IN LYMPHOCYTES OF PATIENTS WITH CERVICAL INSUFFICIENCY
Darko GRUJIČIĆ1, Slobodan ARSENIJEVIĆ2, Dragoslav MARINKOVIĆ3,
Olivera MILOŠEVIĆ-DJORDJEVIĆ1,2
1 Faculty of Science, University of Kragujevac, Kragujevac, Serbia
2 Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
3 Serbian Academy of Sciences and Arts, Belgrade, Serbia
Abstract
Grujičić D., S.Arsenijević, D.Marinković, O. Milošević Đorđević (2013): Inter-individual variance in micronuclei frequency in lymphocytes of patients with cervical insufficiency. Genetika vol 45, No2, 433-439.
The aim of this work was to explain the inter-individual variance in baseline and induced micronuclei (MN) frequency in peripheral blood lymphocytes (PBLs) of females with uterine cervical insufficiency. The baseline and induced MN response of the PBLs in 32 patients were investigated using the cytokinesis-block micronucleus (CBMN) method. The obtained mean values of induced MN frequency (11.31 ± 4.44/1000 BN cells), were significantly higher (p < 0.001) in comparison to their baseline MN frequencies (7.81 ± 3.33 MN/1000 BN cells). The analysis of variance (ANOVA) showed statistically significant difference between-group vs. within-group variance in baseline (p < 0.05) and induced MN frequency (p < 0.001), in professionally exposed patients when compared to the non-exposed. Multivariate logistic regression analysis confirmed that patients with high baseline MN frequency (cut-off 8.5 MN/1000 MN), had significantly increased (odds ratio OR = 5.875; CI = 1.28 - 26.795, p < 0.05) induced MN frequency (>12 MN/1000 BN cells). Our results suggest that the baseline MN frequency in PBLs directly influences the scope of the induced MN response.
Key words: cervical insufficiency, micronuclei, peripheral blood lymphocytes, variability
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 441- 450
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
ENVIRONMENT EFFECT ON DIVERSITY IN QUALITY AND QUANTITY OF ESSENTIAL OIL OF DIFFERENT WILD POPULATIONS OF KERMAN THYME
Abdollah GHASEMI PIRBALOUTI1,2*, Mehdi BARANI1,
Behzad HAMEDI1, Mehrdad ATAEI KACHOUEI1, and Abnoos KARIMI1
1Department of Medicinal Plants, Shahrekord Branch, Islamic Azad University, P.O. Box: 166, Shahrekord, Iran
2Medicinal Plants Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA
Abstract
Pirbalouti Ghasemi A.., M. Barani, B. Hamedi, M. Ataei Kachouei and A. Karimi (2013): Environment effect on diversity in quality and quantity of essential oil of different wild populations of kerman thyme. Genetika, Vol 45, No. 2, 441-450.
Thymus (thyme) is one of the most important genera with regard to the number of species within the family Lamiaceae. Kerman thyme (Thymus carmanicus Jalas) is an endemic Iranian species, intensively utilized because of its wide ranging medicinal and culinary properties. Aerial parts of T. carmanicus collected from various altitudes including 2000-2500, 2500-3000, and 3000-3500 m above sea level in Zagros Mountains, Kerman province, South Iran. The yellow oil yields ranged between 0.80 to 1.10% (v/w) for populations collected from various elevations and for the populations collected from various regions ranged between 0.55-1.61% (v/w). GC-MS analyses revealed compounds, constituting 92.2-99.9% of total essential oils. The major constituents of essential oils were carvacrol (47.6-57.9%), thymol (8.3-19.0%), ɣ-terpinene (7.3-7.9%) and p-cymene (4.4-7.6%), that monoterpenes, especially oxygenated monoterpenes was the main constituent group in essential oil from the aerial parts of T. carmanicus. The results of current study indicated that increasing elevation decreased thymol content in essential oils of the wild populations of T. carmanicus.
Key words: Thymus carmanicus Jalas., essential oil, high altitude, thymol, carvacrol
Corresponding author: Abdollah Ghasemi Pirbalouti, Department of Medicinal Plants, Shahrekord Branch, Islamic Azad University, P.O. Box: 166, Shahrekord, Iran, E-mail: ghasemi@iaushk.ac.ir or aghasemipir@psis.umass.edu ,Tel: +98 (381) 336 10 60,Fax: +98 (381) 336 10 60
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 451- 458
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302451C
Original scientific paper
CELL CYCLE ANALYSIS IN PATIENTS WITH FANCONI ANEMIA FROM SERBIA
Sanja ĆIRKOVIĆ 1 *, Tatjana SRDIĆ-RAJIĆ 2, Marija GUĆ-ŠĆEKIĆ 1, 3, Dragana VUJIĆ 1, 4 , Dragan MIĆIĆ1 and Dejan ŠKORIĆ5
1 Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Belgrade
2 Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
3 University of Belgrade, Faculty of Biology, Serbia
4 University of Belgrade, Faculty of Medicine, Serbia
5 University Children’s Hospital, Belgrade, Serbia
Abstract
Ćirković S.., T. Srdić Rajić, M. Guč Šćekić, D. Vujić, D. Micić and D. Škorić (2013): Cell cycle analysis in patients with Fanconi anemia from Serbia. Genetika, Vol 45, No. 2, 451-458.
Fanconi anemia (FA) is a rare autosomal recessive disorder, characterized by progressive bone marrow failure, chromosomal instability and cell cycle blockage in the G2 phase. The hypersensitivity of FA cells can be additionally induced with specific alkylating agents such as diepoxybutane (DEB) and mitomycin C, which is used in differential diagnosis of FA. Among 72 patients with clinical suspicion of FA, who were diagnosed at the Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic” and the University Children’s Hospital (2004 – 2011), in 10 patients the diagnosis of FA was confirmed on the basis of an increased chromosome sensitivity to DEB. Five out of 10 FA patients were available for further flow cytometric analysis of cell cycle. We examined cell cycle blockage in G2 phase in untreated and with DEB treated lymphocyte cultures from FA patients and from the healthy persons, as control group. All five patients affected with FA, showed an increased DEB induced G2-phase-blockage which was over two times higher than in controls. The percentage of FA cells arrested in G2 phase was between 4,41% and 10,45% with mean value (MV) of 7,76%, but in the control group this range was lower: 1,56% - 4,11% (MV: 2.84%), with no overlapping. FA patients showed an increased spontaneous arrest in G2 phase, as well, comparing to healthy controls (MV: 14,63% vs. 5,82%). Cell cycle assay of G2 phase blockage could be used as an additional diagnostic tool for confirmation of FA in patients with clinical suspicion of this disease.
Key words: Fanconi anemia, cell cycle, G2 phase, diepoxybutane
Corresponding author: Sanja Ćirković, Laboratory for Medical Genetics, Mother and Child Health Care Institute of Serbia “Dr Vukan Cupic”, Radoja Dakica 6-8, 11070 Belgrade, phone: ++ 381 11 3108 273,e-mail: genetikaimd@beotel.rs, sanja.s.cirkovic@gmail.com
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 459-466
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
DOI:
10.2298/GENSR1302459R
Original scientific paper
CORRELATIONS AND PATH ANALYSES OF YIELD AND OTHER SUNFLOWER SEED CHARACTERS
Velimir RADIĆ, Jelena MRĐA, Sreten TERZIĆ, Boško DEDIĆ, Aleksandra DIMITRIJEVIĆ,
Igor BALALIĆ, Dragana MILADINOVIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Radić V., J. Mrđa, S. Terzić, B. Dedić, A. Dimitrijević, I. Balalić and D. Miladinović (2013): Correlations and path analyses of yield and other sunflower seed characters. Genetika, Vol 45, No. 2, 459-466.
Ten sunflower commercial female parental lines were evaluated for various parameters under field conditions to estimate genetic parameters, correlation coefficient, and path analyses. Interactions of seed germination, 1000 seed weight, oil and protein content with seed yield were examined. After three years of observations, positive significant correlations were found while comparing seed yield with 1000 seed weight. Negative significant correlations were found by comparing seed yield and seed germination. Positive but not significant correlation was found while comparing seed yield with oil content in sunflower seed. Path coefficient analysis indicates that 1000 seed weight has maximum positive and seed germination maximum negative direct effect on yield.
Key words: sunflower, seed and yield components, simple correlation coefficient, path coefficient analysis
Corresponding author: Velimir Radić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad 21000, Serbia, email: velimir.radic@nsseme.com
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 467-478
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634.11
DOI:
10.2298/GENSR1302467G
Original scientific paper
GENETIC STRUCTURE OF APPLE ACCESSIONS MAINTAINED EX SITU IN BOSNIA AND HERZEGOVINA EXAMINED BY MICROSATELLITE MARKERS
Fuad GAŠI1, Maja ŽULJ MIHALJEVIĆ2, Silvio ŠIMON2, Jasmin GRAHIĆ1, Naris POJSKIĆ3
Mirsad KURTOVIĆ1, Dragan NIKOLIĆ4, Ivan PEJIĆ2
1 Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
2Department of Plant Breeding, Genetics and Biometrics , Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
3Laboratory for molecular genetics of natural resources, Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
4Institute for fruit science and viticulture, Faculty of Agriculture, University of Belgrade, Beograd – Zemun, Serbia.
Abstract
Gaši F., M. Žulj Mihaljević, S. Šimon, J. Grahić, N. Pojskić, M. Kurtović, D.Nikolić and I. Pejić (2013): Genetic structure of apple accessions maintained ex situ in Bosnia and Herzegovina examined by microsatellite markers. Genetika, Vol 45, No. 2, 467-478.
In order to identify possible mislabeling of the apple accession maintained ex situ in Srebrenik and to gain insight into the genetic structure of the conserved germplasm, 14 accessions from the collection were genotyped using 10 SSR (Simple Sequence Repeats) markers. Obtained SSR profiles were then added to an existing database constructed for previously characterized 24 traditional and 13 international, reference apple cultivars maintained at the same collection. Bayesian analysis implemented in the STRUCTURE program grouped 42 out of 51 analyzed apple accessions (38 traditional and 13 international) into three RPPs (reconstructed panmictic populations) with probability of membership qI higher than 75%. Almost all international, reference cultivars grouped in RPP3, whereas traditional B&H cultivars from the Srebrenik collection grouped in all three RPPs. Large and significant differentiations between all three individual RPPs were detected through the analyses of molecular variance and confirmed with FCA (factorial correspondence analyses). NJ cluster analysis, based on the Bruvo genetic distance, revealed that out of 38 traditional B&H apple cultivars, analyzed in the study, ‘Ljepocvjetka’, ‘Bobovec’ and ‘Bobovec J’ grouped closest to the international reference cultivars. Available date indicates that unlike a large number of B&H apple cultivars which were introduced during the reign of the Ottoman Empire, ‘Ljepocvjetka’ and ‘Bobovec’ were probably introduced at a later date. Cluster analyses also enabled the detection of one synonym and three homonyms within the collection. In four cases, previously conducted identification based on phenotypic analyses was confirmed by genetic analyses. Results of the structure analyses indicate a heterogeneous genetic structure of the analyzed accessions. This characteristic of the B&H apple germplasm could be useful for future breeding programs.
Key words: traditional apple cultivars, SSR, factorial correspondence analyses, Bayesian analyses
Corresponding author: Fuad Gasi, PhD; Faculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina. Telephone number: +387 33 653 033; Fax number: +387 33 667 429; E mail: fudo01@yahoo.com.
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 479-492.
© 2013Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI: 10.2298/GENSR1302479V
Original scientific paper
MOLECULAR ANALYSIS OF ENTEROLYSIN A AND ENTL GENE CLUSTER FROM NATURAL ISOLATE Enterococcus faecalis BGPT1-10P
Katarina VELJOVIĆ*, Amarela TERZIĆ-VIDOJEVIĆ, Maja TOLINAČKI, Milan KOJIĆ
and Ljubiša TOPISIROVIĆ
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Serbia
Abstract
Veljović K., A. Terzić-Vidojević, M. Tolinački, M. Kojić and Lj. Topisirović (2013): Molecular analysis of enterolysin a and entl gene cluster from natural isolate Enterococcus faecalis bgpt1-10p. Genetika, Vol 45, No. 2, 479-492.
Strain Enterococcus faecalis BGPT1-10P was isolated from artisanal semi-hard homemade cheese from Stara Planina, Serbia. Results showed that BGPT1-10P synthesized a heat labile bacteriocin with a broad spectrum of activity, including Listeria and Candida species. Further analysis revealed that synthesized bacteriocin is enterolysin A. Moreover, the entL gene encoding enterolysin A was found to be located on the chromosome. The entL gene was cloned and sequenced. Analysis of nucleotide sequence showed that the entL gene in natural isolate En. faecalis BGPT1-10P is identical to that of the entL gene described previously in En. faecalis LMG 2333. Within the cloned DNA fragment containing the entL gene, four ORFs were detected. One of them was identified as the scpE gene, which encodes a virulent factor staphopain peptidase. Functional analysis of the entL gene showed that the complete genetic information necessary for the synthesis of enterolysin A were directly linked solely to it. Strain BGPT1-10P also synthesized gelatinase and citolysin, and contained a set of virulent factors. In addition, BGPT1-10P carries the ermB and tetM genes conferring the resistance to erythromycin and tetracycline, respectively.
Key words: Enterococcus faecalis / enterolysin A / scpE gene / antibiotic resistance.
Corresponding author: Katarina Veljović, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia,E-mail: lab6@imgge.bg.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 493-502.
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
DOI:
10.2298/GENSR1302493K
Original scientific paper
GENETIC RELATIONSHIPS AMONG SOME Pinus, Picea AND Abies SPECIES REVEALED BY RAPD MARKERS
Dragan KOVAČEVIĆ 1*, Biljana NIKOLIĆ 2, Snežana MLADENOVIĆ DRINIĆ1,
Srđan BOJOVIĆ 3, Tanja DODOŠ 4, Nemanja RAJČEVIĆ 4, and Petar D. MARIN 4
1*Maize Research Institute “Zemun Polje”, Belgrade, Serbia,
2Institute of Forestry, Belgrade, Serbia
3University of Belgrade, Institute for Biological Research “Siniša Stanković”,
Belgrade, Serbia
4University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden
“Jevremovac“,Belgrade, Serbia
Abstract
Kovacevic D, B. Nikolic, S. Mladenovic Drinic, S. Bojovic, T.Dodoš, N.Rajčević and P. Marin (2013): Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers. Genetika, Vol 45, No. 2, 493-502.
Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between three Pinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. Genus Picea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative- morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera - Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus.
Key words:Serbian spruce, Bosnian pine, Macedonian pine, RAPD markers
Corresponding author: Dragan Kovačević, Maize Research Institute “Zemun Polje”, S. Bajića 1, 11185 Belgrade, Serbia, e-mail: dragan.kovacevic@hotmail.com, fax: +381 11 37 56 707
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 503-514
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302503G
Original scientific paper
INVESTIGATION OF FIVE POLYMORPHIC DNA MARKERS ASSOCIATED WITH LATE ONSET ALZHEIMER DISEASE
Jalal GHARESOURAN1, Maryam REZAZADEH1,2, Seiied MOJTABA MOHADDES ARDEBILI1
1Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Abstract
Gharesouran J, M. Rezazadeh, S. Mojtaba Mohaddes Ardebili (2013): Investigation of five polymorphic DNA markers associated with late onset Alzheimer disease . Genetika, Vol 45, No. 2, 503-514.
Alzheimer's disease is a complex neurodegenerative disorder characterized by memory and cognitive impairment and is the leading cause of dementia in the elderly. The aim of our study was to examine the polymorphic DNA markers CCR2 (+190 G/A), CCR5Δ32, TNF-α (-308 G/A), TNF-α (-863 C/A) and CALHM1 (+394 C/T) to determine the relationship between these polymorphisms and the risk of late onset Alzheimer's disease in the population of Eastern Azerbaijan of Iran. A total of 160 patient samples and 163 healthy controls were genotyped by PCR-RFLP and the results confirmed using bidirectional sequencing.
Statistical analysis of obtained data revealed non-significant difference between frequency of CCR5Δ32 in case and control groups. The same result was observed for TNF-α (-863 C/A) genotype and allele frequencies. Contrary to above results, significant differences were detected in frequency of TNF-α (-308 G/A) and CCR2-64I genotypes between the cases and healthy controls. A weak significant difference observed between allele and genotype frequencies of rs2986017 in CALHM1 (+394 C/T; P86L) in patient and control samples.
It can be concluded that the T allele of P86L variant in CALHM1 & +190 G/A allele of CCR2 have a protective role against abnormal clinical features of Alzheimer's disease.
Key words: Alzheimer disease; CCR2; CCR5; CALHM1; TNF-α; Eastern Azerbaijan.
Corresponding author: Seiied Mojtaba Mohaddes Ardebili, Head department of medical genetics,Faculty of medicine,Tabriz university of medical sciences, Tabriz-Iran Tel/Fax: ++98-411-3371587,e-mail: mohaddesmo@yahoo.com
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 515-526
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302515D
Original scientific paper
MITOCHONDRIAL DNA CONTROL REGION VARIABILITY IN WILD BOARS FROM WEST BALKANS
Mihajla DJAN1, NevenaVELIČKOVIĆ1, Dragana OBREHT1, Nataša KOČIŠ TUBIĆ1, Vladimir MARKOVIĆ2, Milan STEVANOVIĆ3, Miloš BEUKOVIĆ4
1University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia
2University of Novi Sad, Faculty of Sciences, Department of Geography, Tourism and Hotel Management, Novi Sad, Serbia
3Maize Research Institute “Zemun Polje”
4University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
Abstract
Djan M, N. Veličković, D. Obrehti, N. Kočiš Tubić, V. Marković, M.Stevanović and M.Beuković (2013): Mitochondrial DNA control region variability in wild boars from west Balkans. Genetika, Vol 45, No. 2, 515-526.
The wild boar (Sus scrofa) is one of most abundant game species in hunting areas of Balkan region. The large fraction of pre-glacial genetic diversity in wild boar populations from the Balkans was addressed due to high proportion of unique mtDNA haplotypes found in Greece, indicating Balkan as main refugial area for wild boars. The aim of the present study is to characterize mitochondrial DNA control region variability in wild boars from different areas in the West Balkan region, in order to evaluate level of genetic variability, to detect unique haplotypes and to infer possible structuring. The total number of 163 individuals from different sampling localities were included in the study. A fragment of the mtDNA control region was amplified and sequenced by standard procedures. Population genetic analyses were performed using several computer packages: BioEdit, ARLEQUIN 3.5.1.2., Network 4.6.0.0 and MEGA5. Eleven different haplotypes were identified and haplotype diversity was 0.676, nucleotide diversity 0.0026, and the average number of nucleotide differences (k) 1.169. The mismatch distribution and neutrality tests indicated the expansion of the all populations. It is shown that high level of genetic diversity is present in the wild boars from the West Balkan region and we have managed to detect regional unique haplotypes in high frequency. Genetic diversity differences have been found in regional wild boar groups, clustering them in two main clusters, but further speculations on the reasons for the observed clustering are prevented due to restricted informativness of the single locus marker. Obtained knowledge of genetic variation in the wild boar may be relevant for improving knowledge of the phylogeny and phylogeography of the wild boars, but as well as for hunting societies and responsible authorities for the effective control of wild boar populations.
Key words: wild boar, mtDNA control region, West Balkan
Corresponding author: Mihajla Djan, University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia, Tel: +381 21 485 2799,Fax: +381 21 450 620,e-mail: mihajla.djan@dbe.uns.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 527-536
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
DOI:
10.2298/GENSR1302527S
Original scientific paper
SSR DNA MARKER AIDED GENETIC DIVERSITY ASSESSMENT OF SELECTED WILLOW CLONES
N.B. SINGH*, S. JOSHI**, P. CHOUDHARY*** & J P SHARMA**
* Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan (H. P.) India
** Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan (H. P.) India
*** Agro Forestry, KVK, Rajouri, Sher- e- Kashmir University of Agricultural Sciences and Technology of Jammu (J&K), India
Abstract
Sing N.B., S. Joshi, P. Choudhary and J.P. Sharme (2013): SSR DNA marker aided genetic diversity assessment of selected willow clones. Genetika, Vol 45, No. 2, 527-536.
Around 100 clones of tree willows were subjected for nursery screening twice on morphometric traits. Genetic diversity was assessed in twenty-five genetically superior willow clones hailing from six countries using 16 SSR primers. Fourteen primers amplified the DNA but only ten showed polymorphism. Total 34 bands were scored, out of that 27 were found to be polymorphic and 7 were monomorphic. Three primers showed 100% polymorphism whereas 79.4% polymorphism was recorded in total. The dendrogram obtained from SSR markers revealed that clone SE-69-002 (S. matsudana) and NZ-1040 (S. matsudana X S. alba) as most similar clones (Jaccards coefficient of 0.97), and clone PN-721(S. matsudana X S. alba) and PN-731 (S. nigra), as most divergent clones (Jaccards coefficient of 0.63). All the genotypes were grouped into 4 distinct clusters. On the basis of similarity coefficient analysis the first cluster comprised of 11 genotypes, the second cluster have 8 genotypes where as third one has only one genotype and fourth cluster retained five genotypes. The clustering pattern further indicated that the geographic distribution may not be the reflection of genetic diversity in willow clones. Genotypes with high molecular diversity could be used in breeding programme in order to obtain heterotic hybrids and development of gene pools with broad genetic base. The genotype specific bands developed by the SSR primers could also be used for identification of cultivar.
Key words: Simple Sequence Repeats, Genetic diversity, dendrogram, polymorphism, Salix, cluster.
Corresponding author: J P Sharma ,Agro Forestry, KVK, Rajouri, Sher- e- Kashmir University of Agricultural Sciences and Technology of Jammu (J&K), India Email : pmd.choudhary@gmail.com
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 537-552
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR13022537V
Original scientific paper
SEXUAL SELECTION CAN REDUCE MUTATIONAL LOAD IN Drosophila subobscura
Marija SAVIĆ VESELINOVIĆ1, Sofija PAVKOVIĆ-LUČIĆ1, Zorana KURBALIJA NOVIČIĆ2, Mihailo JELIĆ1, Marko ANĐELKOVIĆ1,2,3
1 Faculty of Biology, University of Belgrade, Belgrade, Serbia
2 Institute for Biological Research “Siniša Stanković”, University of Belgrade,
Belgrade, Serbia
3 Serbian Academy of Sciences and Arts, Belgrade, Serbia
Abstract
Veselinović Savić M., S. Pavković Lučić, Z. Kurbalija Novičić, M. Jelić and M. Anđelković (2013): Sexual selection can reduce mutational load in Drosophila subobscura. Genetika, Vol 45, No. 2, 537-552.
According to theoretical predictions sexual selection can reduce mutational load through male mating success. Males of good genetic quality should be more successful in matings, compared to the males of low genetic quality, thus in this way females can prevent deleterious alleles to be transmitted to the next generation. We tested this hypothesis through set up of two experimental groups from same genetic pool, where in one group genetic quality was manipulated by ionizing radiation. Within each group opportunity for choosing mates was imposed: males and females had no choice or had multiple choice. Mutational load was measured through the variability of different fitness components: fecundity and egg-to-adult viability. Our results indicate that sexual selection can reduce mutational load, only for fecundity. Group with the presence of female choice exhibited higher fecundity than group in which sexual selection was experimentally eliminated, but only in “irradiated” group. There was no overall difference in egg-to-adult viability between different sexual selection regimes in any of the group. It should be considered that sexual selection can cause sexual conflict, and potential opposite effects of sexual selection and sexual conflict on fitness. Genetic structure of populations, in terms of the level of mutational load, is an important factor which can determinate the role of sexual selection.
Key words: deleterious mutations, ionizing radiation, sexual conflict, fitness
Corresponding author: Marija Savić Veselinović, Studentski trg 16, 11000 Belgrade, Phone: +381 11 2187 266, Fax: +381 11 2638 500, mob: +381 64 1948 923, E-mail: marijas@bio.bg.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 553-563
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575:633
DOI:
10.2298/GENSR1302553L
Original scientific paper
GENETIC VARIABILITY OF SEED YIELD AND SEED YIELD COMPONENTS OF AUTOCHTHONOUS Lolium perenne L. POPULATIONS
1Zeljko LAKIC, 2Dejan SOKOLOVIC, 2 Snezana BABIC, 1Svetko VOJIN, 3
Jela IKANOVIC, 3Tatjana Veljovic, 4Igor BALALIC
1Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia and Herzegovina
2 Institute for forage crops, Krusevac, Serbia
3 University of Belgrade, Faculty of Agriculture, Belgrade, Serbia
4 Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Lakić Z, D. Sokolović, S. Babić, S. Vojin, J. Ikanović, T. Veljović and I. Balalić (2013): Genetic variability of seed yield and seed yield components of autochthonous Lolium perenne L. populations. Genetika, Vol 45, No. 2, 553-563.
The aim of this study was to examine genetic variability, heritability and correlation of seed yield components and seed yield of progenies of autochthonous populations and cultivars of perennial ryegrass, and that on the basis of these results distinguish genotypes that would be later used in the creation of new local cultivars of perennial ryegrass. Research was carried out on experimental fields and laboratories of the Agricultural Institute of Republic of Srpska in Banja Luka in 2007. and 2008., on 6 natural populations of perennial ryegrass collected in Bosnia and Herzegovina and two cultivars, Maja and Calibra as standard. Following parameters were analyzed: number of generative tillers per plant, time of flowering, spike length, number of spikelets per spike, seed yield per spike and plant and 1000 seed weight.
Analysis of the results of the number of generative tillers per plant indicates a statistically significant difference in the studied populations and cultivars of perennial ryegrass. The highest average number of tillers per plant was found in the cultivar Maja (193.8), and the least number was detected in population Dragočaj (78.9), so statistical differences were highly significant. The longest period to beginning of flowering was determined in cultivar Calibra (56.5 days) and the shortest in population Laminci (43 days). The average length of spike of perennial ryegrass in the studied population was 22.78 cm and was significantly lower than cultivar Maja. Statistically significant differences between populations and variety Maja in the number of spikelets per spike were detected. The average seed yield per spike of studied populations was in level with cultivar Maja and 37.5% higher compared with variety Calibra. In the studied populations of perennial ryegrass seed yield ranged from 5.21 g (Dragočaj population) to 15.40 g (Kupres population). Weight of 1000 seeds was highest in the variety Calibra (2.60 g) and lowest in population Maglajani (1.94 g).
Proportion of genetic to phenotypic variance for time of flowering, the number of generative tillers, seed yield per spike and plant and 1000 seed weight indicate that the variability of these traits in this collection of genotypes, largely derived from plant genotype.
In this investigation, the presence of a highly significant positive genetic correlation was found between time of flowering and spike length (0.98), time of flowering and 1000 seed weight (0.97), number of generative tillers and seed yield per plant (0.91) and spike length and 1000 seed weight (0.98).
Key words: perennial ryegrass, population, cultivar, seed yield, seed yield components
Corresponding author: Zeljko Lakic, Agricultural Institute of Republic of Srpska, Knjaza Milosa 17, 78 000 Banja Luka, Republic of Srpska - Bosnia and Herzegovina, tel: 00387 51 303 112, fax: 00387 51 312 792, e-mail: zeljko_lakic@inecco.net
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 565-574
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
DOI:
10.2298/GENSR1302565S
Review paper
OILSEED RAPE GENOTYPES RESPONSE TO BORON TOXICITY
Jasna SAVIĆ1, Ana MARJANOVIĆ-JEROMELA2, Đorđe GLAMOČLIJA1,
Slaven PRODANOVIĆ1
1Faculty of Agriculture, University of Belgrade, Zemun-Belgrade, Serbia
2Institute for Field and Vegetable Crops, Novi Sad
Abstract
Savić J, A. Marijanović Jeromela, Đ. Glamočlija, and S. Prodanović (2013): Oilseed rape genotypes response to boron toxicity. Genetika, Vol 45, No. 2, 565-574
Response of 16 oilseed rape genotypes to B (boron) toxicity was analyzed by comparing the results of two experiments conducted in a glasshouse. In Experiment 1 plants were grown in standard nutrient solutions with 10 μM B (control) and 1000 μM B. Relative root and shoot growth varied from 20-120% and 31-117%, respectively. Variation in B concentration in shoots was also wide (206.5-441.7 µg B g-1 DW) as well as total B uptake by plant (62.3-281.2 µg B g-1). Four selected genotypes were grown in Experiment 2 in pots filled with high B soil (8 kg ha-1 B; B8). Shoot growth was not affected by B8 treatment, while root and shoot B concentration was significantly increased compared to control. Genotypes Panther and Pronto which performed low relative root and shoot growth and high B accumulation in plants in Experiment 1, had good growth in B8 treatment. In Experiment 2 genotype NS-L-7 had significantly lower B concentration in shots under treatment B8, but also very high B accumulation in Experiment 1. In addition, cluster analyses classified genotypes in three groups according to traits contrasting in their significance for analyzing response to B toxicity. The first group included four varieties based on their shared characteristics that have small value for the relative growth of roots and shoots and large values of B concentration in shoot. In the second largest group were connected ten genotypes that are heterogeneous in traits and do not stand out on any characteristic. Genotypes NS-L-7 and Navajo were separated in the third group because they had big relative growth of root and shoot, but also a high concentration of B in the shoot, and high total B uptake. Results showed that none of tested genotypes could not be recommended for breeding process to tolerance for B toxicity.
Key words: B toxicity, oilseed rape, B concentration, nutrient solution, cluster analyses
Corresponding author: Jasna Savić, Faculty of Agriculture, University of Belgrade, 11080 Zemun-Belgrade, Serbia, phone ++381 64 220 44 71, fax: ++ 381 11 3161987, e-mail: jaca@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 575-588
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
DOI:
10.2298/GENSR1302575M
Review
paper
EFFECTIVE POPULATION SIZE ESTIMATION IN SEED ORCHARDS: A CASE STUDY OF Pinus nigra ARNOLD AND Fraxinus excelsior L./ F. angustifoliA VAHL.
Eva MACHANSKÁ1), Vladimír BAJCAR1), Roman LONGAUER1)2), Dušan GÖMÖRY3)*
1) National Forestry Centre, Forestry Research Institute, Zvolen, Slovakia
2) Mendel University, Faculty of Forestry and Wood Technology, Brno, Czech Republic
3) Technical University in Zvolen, Faculty of Forestry, Zvolen, Slovakia
Abstract
Machanska J, V. Bajcar, R. Longauer, and D. Gomory (2013): Effective population size estimation in seed orchards: a case study of Pinus nigra arnold and Fraxinus excelsior L./ F. angustifolia vahl.Genetika, Vol 45, No. 2, 575-588
Effective population size as a parameter closely correlating with the genetic and genotypic diversity of the seed orchard output is an important indicator of seed orchard functioning. It is determined by the variation of male and female gametic contributions of parental genotypes (including those outside the seed orchard), influenced by the variation in male and female gamete production, reproductive phenology, pollen dispersal within seed orchard and other factors. We assessed male and female fecundity, as well as temporal course of male and female flowering in two seed orchards of Pinus nigra Arnold and Fraxinus excelsior L. / F. angustifolia Vahl. in Slovakia. In both cases, male and female gametic contributions of plus-tree clones were modeled on the basis of fecundity and flowering phenology, and were used to calculate status number as an estimator of effective population size.
In the seed orchard of Pinus nigra, marker-aided verification of clonal fidelity revealed unexpectedly high proportion of misplaced ramets (29.9%) and alien genotypes (44.4%). Monitoring of reproductive processes in 2002 and 2003 showed high variation in both male and female fecundity, and pollen shedding preceding female receptivity in Pinus nigra. All these factors contributed to a very low relative status effective number, representing 8.6% to 38.6% of the population census (depending from the management option in relation to misplaced and alien genotypes).
In the mixed seed orchard of Fraxinus excelsior and F. angustifolia, the proportion of misplaced and alien genotypes was much lower (22.4% and 12.3%, respectively). However, a high fecundity variation and protogyny resulted in a low relative status number (18.8% to 29.5% of the census number of clones) also in this seed orchard. Practical implications of these findings are shortly discussed and practical management options are proposed.
Key words: B toxicity, oilseed rape, B concentration, nutrient solution, cluster analyses
Corresponding author: Dušan Gömöry, Technical University in Zvolen, Faculty of Forestry, T.G. Masaryka 24, 960 53 Zvolen, Slovakia, phone +421-45-5206226, e-mail gomory@tuzvo.sk
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 589-599
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
DOI:
10.2298/GENSR1302589B
Review paper
The identification of bands related to yields and stability in maize hybrids and their parental components
Vojka BABIĆ1, Slaven PRODANOVIĆ2, Milosav BABIĆ1, Nenad DELIĆ1,
Violeta ANĐELKOVIĆ1
1Maize Research Institute, Zemun Polje, Belgrade, Serbia
2Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Abstract
Babić V., S. Prodanović, M. Babić, N. Delić and V. Anđelković (2013): The identification of bands related to yields and stability in maize hybrids and their parental components. Genetika, Vol 45, No. 2, 589-599.
Geneticists have been trying to explain adaptability and stability of a genotype in terms of a desirable combination of alleles expressed via epistasis. Stability determined in one set of data is very often stability of a genotype to a prevalent stress factor in a moment of carrying out the experiment. However, grain stability of a certain genotype can be a result of different factors, such as tolerance to drought, or to some important diseases and pests. Yield and yield stability of 15 maize hybrids were observed in 24 environments. The level of the interaction effect of studied maize hybrids was identified by the AMMI analysis, while a number of bands positively related to yield and stability of studied maize hybrids were identified by a genetic characterisation by RAPD markers. Bands positively related to yields were present to a greater extent in parents originating from the BSSS population, while bands positively related to stability were more present in parents originating from the Lancaster population.
Key words: maize hybrids, RAPD markers, yield stability
Corresponding author: Babić Vojka, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia; Tel: +38111 37 56 704; Fax: +38111 37 56 707; e-mail: vbabic@mrizp.rs
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 601-610
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:634
DOI:
10.2298/GENSR1302601T
Review
paper
BREEDING EVALUATION OF PEPPER LINES OBTAINED
BY IN VITRO ANTHER CULTURE
Velichka TODOROVA*, Stanislava GROZEVA, Velichka RODEVA, Stoyka MASHEVA
Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria
Abstract
Todorova V., S. Grozeva, V. Rodeva, and S. Masheva (2013): Breeding evaluation of pepper lines obtained by in vitro anther culture. Genetika, Vol 45, No. 2, 601-610.
The comparative breeding evaluation was carried out with six advanced pepper lines (Capsicum annuum L.) with androgenic origin and their initial pepper variety Hebar during the period 2008 – 2010 in field conditions at Plovdiv, Bulgaria. The genotypes were characterized by total and standard yield, plant height and fruit traits: length, width, wall thickness and weight. The response of two lines (4 and 6) and control variety to the Verticillium wilt was also investigated on artificial infected background. According to the results of the experimental work standard and total yield in all androgenic lines were higher compared to the initial variety but significant differences were established only for lines 1, 2, 4 and 6. Line 6 was characterized with the highest standard yield (4009 kg/da) followed by line 2 (3829 kg/da). They exceeded variety Hebar with 42.26% and 35.89%, respectively. Line 2 formed the heaviest (61.31 g) and longer (11.24 cm) fruits than most studied genotypes. Anther-derived lines were also with a good uniformity by studied traits. In line 6 was registered lower index of infestation by Verticillium dahliae Kleb. than the control during the investigated period. These perspective lines will be included as valuable genetic resources for future pepper breeding programs for development of new varieties.
Key words: androgenesis, Capsicum, Verticillium, fruit, yield
Corresponding author: Velichka Todorova, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse 32, 4003 Plovdiv, Bulgaria, Tel.: +35932952296, Fax: +35932960177, e-mail: todorova_vili@abv.bg;
Published in „GENETIKA“ Vol. 45, No. 2(2013), pp. 611-619
© 2013 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
DOI:
10.2298/GENSR1302611R
Review
paper
ROLE OF MICA REPEAT POLYMORPHISM IN THE MANIFESTATION OF TYPE 1 Diabetes mellitus IN BENGALI INDIAN PATIENTS
Oindrila RAHA1,2, B.N.SARKAR1, P.VEERRAJU2, G. SUDHAKAR2, P.RAYCHAUDHURI 3, Soma MUKHOPADHYAY4, V.R.RAO5.
1 Anthropological Survey of India, Kolkata, West Bengal, India.
2 Department of Human Genetics, Andhra University, Visakhapatnam-530003, India.
3 Endocrinology Department, Calcutta Medical College & Hospital, Kolkata, India.
4 Netaji Subhash Chandra Bose Cancer Research Institute, Kolkata, India.
5 Department of Anthropology, University of Delhi, Delhi, India.
Abstract
Raha O., B.N. Sarkar, P. Veerraju, G. Sudhakar, P.Raychaudhzri, S. Mukhopadhyay and V.R. Rao (2013): Role of mica repeat polymorphism in the manifestation of type 1 diabetes mellitus in Bengali Indian patients. Genetika, Vol 45, No. 2, 611-619.
Background. The major histocompatibility complex class I chain–related gene A (MICA) (OMIM-600169) is a polymorphic gene in the HLA region expressed mainly by epithelial cells. The MICA protein encoded by the allele influences the activation of NK cells, which modify β-cells destruction and has been found to be involved in susceptibility of T1DM.
Objective. The aim of this study was to find the association of MICA alleles with T1DM among eastern Indian population.
Subjects and methods. Study was conducted in 134 eastern Indian patients and with 137 healthy controls for the possible role of MICA gene in T1DM pathogenesis.
Results. The MICA*A5 microsatellite allele, showed significantly higher frequencies in patients than controls (p=0.003, OR= 1.746, CI= 1.206- 2.528). MICA A*6 was found to be protective in our study (p=<0.01, OR=0.406, CI= 0.268-0.616).
Key words: MICA, T1DM, Bengali, India.
Corresponding author: V. R. Rao, Department of Anthropology, Delhi University, North Campus,Delhi - 110 007,Tel: 91-11-27667329, Fax: 91-11-27666614, Mobile 91-9560839997,e.Mail: ansidiabetes@gmail.com