GENETIKA, Vol. 47, No. 3 (2015)

 

Hasan pinar,  Mustafa unlu,  Sezai ercisli, Aydin uzun,  Mustafa bircan,  Kadir Ugurtan yilmaz

DETERMINATION OF SELF-(IN)COMPATIBILITY IN SOME TURKISH CULTIVATED AND WILD APRICOTS  [Abstract] [Full text]

 

Vera rakonjac, Boban djordjević, Milica fotirić akšić, Todor vulić, Dejan djurović

ESTIMATION OF VARIATION AND CORRELATION ANALYSIS FOR YIELD COMPONENTS IN BLACK CURRANT CULTIVARS [Abstract] [Full text]

 

Yuksel kaya, Mehmet sahin

NON-PARAMETRIC STABILITY ANALYSES OF PROTEIN CONTENT IN MULTI-ENVIRONMENT TRIALS OF WHEAT (T. aestivum L.) [Abstract] [Full text]

 

Nada HLADNI, Vladimir MIKLIČ, Anto MIJIĆ, Siniša JOCIĆ, Dragana MILADINOVIĆ

CORRELATION AND PATH COEFFICIENT ANALYSIS FOR PROTEIN YIELD IN CONFECTIONARY SUNFLOWER (Helianthus annuus L.)  [Abstract] [Full text]

 

Josip čolo, Sanja mihajlović, Maja tolinački, Mersiha alkić, Dušanka popović, Milan kojić, Amarela terzić-vidojević

CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BOSNIAN ARTISANAL DRY FERMENTED SAUSAGE (SUDŽUK) DURING FERMENTATION  [Abstract] [Full text]

 

Bojana petrović, Aleksandar ljubić, Milica komnenić^, Violeta joksimović, Olivera lekić miladinović

FETAL ULTRASOUND FINDINGS IN TRISOMY 18 AT MIDPREGNANCY [Abstract] [Full text]

 

Hamlet Baykishi sadigov

Gliadin and Glutenin polymorphism in durum wheat landraces and breeding varieties of Azerbaijan [Abstract] [Full text]

Vladislava galović, Mirjana šijačić-nikolić, Robert šafhauzer, Dijana čortan, Saša orlović

Genetic differentiation of Norway spruce (Picea abies (L.) Karst.) trees with diferent crown types from the mountain Golija  [Abstract] [Full text]

 

P.SUKUMAR and R.K.GNANAMURTHY

SEGMENTATION AND ABNORMALITY DETECTION OF CERVICAL CANCER CELLS USING FAST ELM WITH PARTICLE SWARM OPTIMIZATION  [Abstract] [Full text]

 

Nenad BUKVIC^, , Dora VARVARA, Cesare ROSSI, Maria Felicia FAIENZA, Francesco C. SUSCA, Nicoletta RESTA

From clinical suspect to molecular confirmation of Noonan syndrome; contribution of “best practice” genetic counseling and new technical possibilities  [Abstract] [Full text]
 

Susana GONZÁLEZ–PÉREZ¹, Cristina MALLOR², Ana GARCÉS–CLAVER², Fuencisla MERINO¹, Alfredo TABOADA³, Antonio RIVERA³, Federico POMAR¹, Dragan PEROVIC^4,5, Cristina SILVAR^1*

EXPLORING GENETIC DIVERSITY AND QUALITY TRAITS IN A COLLECTION OF ONION (Allium cepa L) LANDRACES FROM NORTH-WEST SPAIN [Abstract] [Full text]

 

Serkan dogan^1*, Adna ašić¹, Sanida buljubašić¹, Larisa bešić¹, Monia avdić¹, Elma ferić¹, Mirsada hukić¹, Yusuf turan¹ and Damir marjanović¹

OVERVIEW OF EUROPEAN POPULATION CLUSTERING BASED ON 23 Y-STR LOCI [Abstract] [Full text]

 

Violeta ORO, Nenad MILOVANOVIĆ, Violeta PETROVIĆ, Bogdan NIKOLIĆ, Jovana BLAGOJEVIĆ

MITOCHONDRIAL COI IN PHYLOGENETIC RELATIONSHIPS OF LAIMAPHELENCHUS BELGRADIENSIS (NEMATODA: APHELENCHOIDIDAE)  [Abstract] [Full text]

Hülya SIPAHI^*, Ayşen YUMURTACI, Zafer MERT

DEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS Puccinia triticina [Abstract] [Full text]

 

Nevenka VELICKOVA, Misko MILEV, Tatjana RUSKOVSKA, Biljana PETROVA, Bojana NEDELJKOVIK, Pale GORGIEVA

CYTOGENETIC ABNORMALITIES IN LYMPHOCYTES EVALUATED WITH MICRONUCLEUS ASSAY IN MEDICAL PERSONNEL OCCUPATIONALLY EXPOSED TO IONIZING RADIATION  [Abstract] [Full text]

 

Ivica đalović, Đorđe jocković, Yinglong chen², Goran bekavac, Srđan šeremešić, Goran jaćimović, Milka brdar–jokanović

MAIZE NUTRIENT UPTAKE AFFECTED BY GENOTYPE AND FERTILIZATION [Abstract] [Full text]

 

Aleksandra govedarica-lučić and Goran perković

MINERAL CONTENT IN A SALAD LEAF (Lactuca sativa l.)  DEPENDING OF THE GENOTYPE AND APPLIED AGRICULTURAL MEASURES [Abstract] [Full text]

 

Victor M. salceda, Judith guzmán-rincón, Ma  esther de la rosa and Olga olvera

FOUR DECADES OF INVERSION POLYMORPHISM IN Drosophila pseudoobscura FROM MEXICO  [Abstract] [Full text]

 

Aleksandra patenković, Tatjana savić, Bojan kenig, Zorana kurbalija novičić, Marko anđelković

THE IMPACT OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELD (50 HZ, 0.25 MT) ON FITNESS COMPONENTS AND WING TRAITS OF Drosophila subobscura [Abstract] [Full text]

 

Želmíra balážová, Andrej trebichalský, Zdenka gálová, Radomíra hornyák gregáňová

IMPROVEMENT OF ADVENTITIOUS ORGANOGENESIS FOR REGENERATION OF TRANSGENIC PLANTS IN BLACKBERRY [Abstract] [Full text]

 

Catalina dan, Adriana F. sestras, Calin bozdog, Radu E. sestras

INVESTIGATION OF WILD SPECIES POTENTIAL TO INCREASE GENETIC DIVERSITY USEFUL FOR APPLE BREEDING  [Abstract] [Full text]

 

Darshan D. doshi, Druti B. shah, Krishna M. singh and Rajesh K. patel

COMPARATIVE STUDY OF I/D POLYMORPHISM OF ACE GENE IN DIABETES TYPE-2 PATIENTS AND CONTROL GROUP IN UNRELATED GUJARATI POPULATION [Abstract] [Full text]

 

Vesna škodrić trifunović, Ivana buha, Dragana jovanović, Violeta vučinić, Mihailo stjepanović, Vesna spasovski, Marina andjelković, Miša vreća, Anita skakić, Vladimir gaši and Sonja pavlović

VARIANTS IN VDR AND NRAMP1 GENES AS SUSCEPTIBILITY FACTORS FOR TUBERCULOSIS IN THE POPULATION OF SERBIA  [Abstract] [Full text]

 

Nidhi verma,Sudhir shukla, Kusum yadav, Brij K. mishra and Anu rastogi

BIOCHEMICAL CHARACTERIZATION BASED ON SDS-PAGE ANALYSIS AND CORRELATION AMONG TRAITS IN OPIUM POPPY (Papaver somniferum L.) GERMPLASM [Abstract] [Full text]

 

Gorčin CVIJANOVIĆ, Tanja ADNAĐEVIĆ, Mirjana LENHARDT, Saša MARIĆ

NEW DATA ON STERLET (Acipenser ruthenus L.) GENETIC DIVERSITY IN THE MIDDLE AND LOWER DANUBE SECTIONS, BASED ON MITOCHONDRIAL DNA ANALYSES [Abstract] [Full text]

 

n. chandrakanth, s.m. moorthy, k.m. ponnuvel and v. sivaprasad

IDENTIFICATION OF MICROSATELLITE MARKERS LINKED TO THERMOTOLERANCE IN SILKWORM BY BULK SEGREGANT ANALYSIS AND IN SILICO MAPPING  [Abstract] [Full text]

 

Vera lavadinović, Vukan lavadinović, Ilija djordjević

VARIABILITY OF SEEDLINGS OF DOUGLAS-FIR PROVENANCES INTRODUCED FROM CANADA [Abstract] [Full text]

 

Recep dokuyucu, Bulent gogebakan, Cengiz cevik

CORRESPONDING ERDOSTEINE CHANGES AUTOPHAGY GENES EXPRESSION IN HIPPOCAMPUS ON RHINITIS MEDICAMENTOSA MODEL [Abstract] [Full text]
 

Slađana medić-pap, Dejan prvulović, Ana takač, Slobodan vlajić, Dario danojević, Adam takač, Stevan maširević

INFLUENCE OF TOMATO GENOTYPE TO PHENOLIC COMPOUNDS CONTENT AND ANTIOXIDANT ACTIVITY AS REACTION TO EARLY BLIGHT  [Abstract] [Full text]
 

Hadi habibollahi, Zahra noormohammadi, Masoud sheidai, Farah farahani

GENETIC STRUCTURE OF CULTIVATED FLAX (Linum usitatissimum L.) BASED ON RETROTRANSPOSON-BASED MARKERS [Abstract] [Full text]

 

Elizabeta miskoska - milevska, Zoran T. popovski, Blagica dimitrievska, Katerina bandzo

DNA MICROSATELLITE ANALYSIS FOR  TOMATO GENETIC DIFFERENTIATION  [Abstract] [Full text]

 

ABU-ALMAATY, A. H.; Mary WELSON ZEKRY, Yaseen A. ESSA
USING CYTOGENETIC ANALYSIS RAPD IN DETERMINATION OF GENETIC VARIATIONS AMONG FOUR SPECIES OF ORNAMENTAL FISHES OF FAMILY: Poecilidae (Order: Cyprinodontiform) [Abstract] [Full text]

 

Snežana milošević, Aleksandar cingel, Angelina subotić

AGROBACTERIUM-MEDIATED TRANSFORMATION OF ORNAMENTAL SPECIES: A REVIEW [Abstract] [Full text]

 

 

 

Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 777-794

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

 

 

 

UDC 575.630
                            DOI: 10.2298/GENSR1503777P
                                 Orginal scientific paper

 

 

 

DETERMINATION OF SELF-(IN)COMPATIBILITY IN SOME TURKISH CULTIVATED AND WILD APRICOTS

 

Hasan pinar¹,  Mustafa unlu ¹,  Sezai ercisli²*, Aydin uzun ³,  Mustafa bircan¹,  Kadir Ugurtan yilmaz³

 

¹Ministry of Food, Agriculture and Livestock, Alata Horticultural Research Station Directorate, Erdemli-Mersin, Turkey

²Faculty of Agriculture, Ataturk University, Erzurum, Turkey

³Faculty of Agriculture, University of Erciyes, Kayseri, Turkey

 

 

 

Abstract

Pinar H.,  M. unlu,  S. ercisli, A. uzun,  M. bircan,  K. U.yilmaz (2015): Determination of self-(in)compatibility in some Turkish cultivated and wild apricots. - Genetika, Vol 47, No. 3,777 -794.

In this study, self-(in) compatibility of 34 wild apricot genotypes, called as Sakit apricots, sampled from Sakit valley located in east Mediterranean region of Turkey and 9 well known Turkish apricot cultivars were determined by using molecular methods. Genomic PCR with S-RNase and SFB-specific primers was conducted using the degenerate primers EM-PC2consFD and EM-PC3consRD for the amplification of the second intron region of the S-RNase gene. To amplify the first intron, the fluorescently labelled (JOE) forward primer SRc-F was used in combination with the reverse primer SRc-R. According to the results, S_c (self-compatible) allele is not present in all wild and cultivated materials. All wild genotypes had same S allele (S₆S₁₉) composition indicating they do not carry the S_C-haplotype. These wild materials previously reported self-compatible by fruit set studies on field. Mutations rendering the S-locus non-functional in these genotypes can be supposed and checked in the future. The most important dried apricot cultivars of Turkey such as Hacihaliloglu, Kabaasi, and Cataloglu were observed to be self-incompatible cultivars.

Key words: Prunus armeniaca, S-genotype, SRc-R, SRc-F, allel   

 

Corresponding author: Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: 090 4422312599, Fax: 090 4422360958, e-mail: sercisli@gmail.com

 

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Published in „GENETIKA“ Vol. 7, No. 3 (2015), pp. 785 -794

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

                                        UDC 575.630
DOI: 10.2298/GENSR1503785R
                                 Original scientific paper

 

 

 

ESTIMATION OF VARIATION AND CORRELATION ANALYSIS FOR YIELD COMPONENTS IN BLACK CURRANT CULTIVARS

 

Vera rakonjac*, Boban djordjević, Milica fotirić akšić, Todor vulić, Dejan djurović

 

University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

 

Abstract

Rakonjac V., B.Djordjevic, M.Fotirić Akšić, T.Vulić, D.Djurović(2015): Estimation of variation and correlation analysis for yield components in black currant cultivars. - Genetika, Vol 47, No.3,785-794.

Creating genotypes that will be characterized by high yields, good quality and other favorable agronomic characters is a major objective of most currant breeding programs worldwide. For easier and faster achievement of these goals and identification of superior genotypes suitable for use as parents in future hybridization programs, study of genetic parameters seems to be obligatory. In this regard, the aims of our study were to estimate components of variability and heritability, and do correlation analysis for yield components in order to determine efficient strategies for improving yield in black currant breeding programs. Significant differences between cultivars were established for all studied traits. A high proportion of genotypic variance was found with bush width, no. of shoots per bush, bunch weight and berry weight indicating that genetic improvement for these traits through breeding was achievable. Opposite, seasonal variance was high for bush height, no. of bunch per bush and yield. The high heritability coefficients (0.80-0.94) detected for all traits studied reflect the close agreement between their phenotypic and genotypic values. Also, most pairs of traits were similarly correlated at both phenotypic and genotypic levels. So, yield was significantly and positively correlated with bush height, no of bunch per bush and bunch weight. These results imply a rapid response of black currants to selection. 

Keywords: Components of variability, heritability, phenotypic and genotypic correlation, genetic gain, Ribes nigrum L.

 

Corresponding author: Vera Rakonjac, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: ++ 381 64 2830690, fax: ++ 381 11 2199 805, e-mail: verak@agrif.bg.ac.rs

 

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Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 795- 810

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

 

    UDC 575:630.11
DOI: 10.2298/GENSR1503795K
                                 Original scientific paper

 

 

 

NON-PARAMETRIC STABILITY ANALYSES OF PROTEIN CONTENT

 IN MULTI-ENVIRONMENT TRIALS OF WHEAT (T. aestivum L.)

 

Yuksel kaya^,* Mehmet sahin

 

  Bahri Dagdas International Agricultural Research Institute, Konya, Turkey

 

 

Abstract

Kaya Y. and M.Sahin  (2015): Non-parametric stability analyses of protein content in multi-environment trials of wheat (T. aestivum L.).- Genetika, Vol 47, No. 3, 795 - 810.

According to literature, a detailed paper has not been published yet on using non-parametric stability statistics for evaluating genotypic stability in protein content (PC) of wheat. Thus, this study aimed to investigate the stability for PC of wheat using sixteen non-parametric stability measures (YSD-PC standard deviation, RM-Rank mean, RSD-Rank’s standard deviation, RS-Rank Sum stability statistic, PA-Percentage of adaptability, R₁ and R₂-Range indexes, TOP-Ranking, S_i⁽¹⁾, S_i⁽²⁾, S_i⁽³⁾, S_i⁽⁶⁾, NP_i⁽¹⁾, NP_i⁽²⁾ NP_i⁽³⁾and NP_i⁽⁴⁾ rank statistics, together with Y-PC mean). The study included 13 wheat genotypes, consisting of 5 registered cultivars and 8 breeding lines, selected from National Wheat Breeding Program of Turkey. The genotypes were grown in ten rain-fed environments, representative of major rain-fed wheat-growing areas of Turkey, during 2011-2013 cropping seasons. The ANOVA showed that the effects due to environments (E), genotypes (G) and GE interaction (GEI) were significant (P < 0.01). Spearman’s rank correlation and principal component analyses (PCA) also revealed that two types of associations were found between the stability parameters: the first type included S_i⁽¹⁾, S_i⁽²⁾, S_i⁽³⁾, S_i⁽⁶⁾, NP_i⁽¹⁾, NP_i⁽²⁾ NP_i⁽³⁾, NP_i⁽⁴⁾, RSD and YSD parameters which were related to static stability, whereas the second type consisted of the Y, RM, TOP, PA, RS, R1 and R2 parameters which are related to dynamic concept of stability. Among the 8 breeding lines, G7 and G8 were the best genotypes in terms of both high PC and stability. In conclusion it could be suggested that dynamic non-parametric stability statistics should be used for selecting genotypes with high PC and stable when tested across a wide range of environments.   

Key words: Wheat (T. aestivum L.), protein content, non-parametric stability statistics

 

Corresponding author: Yuksel kaya,,Bahri Dagdas International Agricultural Research Institute, Konya, Turkey,Office Phone: +90 332 355 12 90,Office Fax: +90 332 355 12 88,e-mail: yuksel_k@yahoo.com

 

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Published in „GENETIKA“ Vol. 47, No3 (2015), pp. 811 -818

© 2015Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

  

  UDC 575.630
DOI: 10.2298/GENSR1503811H
                                 Original scientific paper

 

 

 

CORRELATION AND PATH COEFFICIENT ANALYSIS FOR PROTEIN YIELD IN CONFECTIONARY SUNFLOWER (Helianthus annuus L.)

 

Nada HLADNI¹, Vladimir MIKLIČ¹, Anto MIJIĆ², Siniša JOCIĆ¹,

Dragana MILADINOVIĆ¹

 

¹ Institute of Field and Vegetable Crops, Novi Sad, Serbia

² Agricultural Institute Osijek, Osijek, Croatia

 

 

Abstract

Hladni N., S. Jocić, A. Mijić, V. Miklič, D. Miladinović (2015): Correlation and path coefficient analysis for protein yield in confectionary sunflower (Helianthus annuus L.).- Genetika, Vol 47 , No.3, 811-818.

The most important criteria for introducing new confectionary hybrids into the production is high protein yield. Path coefficient analysis was used to obtain information on direct and indirect effects of studied traits (seed oil content, kernel oil content, seed yield, kernel protein content, mass of 1000 seeds, kernel ratio and hull ratio) on protein yield. The research was conducted during three vegetation seasons, on 22 experimental confectionary sunflower hybrids created in the breeding program at the Institute of Field and Vegetable Crops. Strong and very strong correlations were found among the largest number of examined traits. A weak negative interdependence was determined between kernel oil content, kernel protein content, mass of 1000 seeds, hull ratio, and protein yield using the analysis of simple correlation coefficients. Positive but weak correlation was determined between protein yield and seed oil content, and kernel ratio. Very strong positive correlation was determined between protein yield and seed yield (0.468^**). The seed oil content had a very strong direct negative effect on protein yield (DE=-0.734^**). The mass of 1000 seeds had a weak negative direct effect on protein yield. Kernel protein content and kernel oil content demonstrated a weak direct positive effect on protein yield. Path coefficient analysis of protein yield showed a very strong positive direct effect of kernel ratio (DE=1.340^**), seed yield (DE=0.657^**) and hull ratio (DE=0.992^*). These findings confirm the effect of seed yield, kernel ratio, and hull ratio on protein yield, and their importance as the selection criteria in confectionary sunflower breeding.

Key words: confectionary sunflower, correlations, path coefficient analysis, protein yield, yield components.

 

Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: +381 21 4898 411; Fax: +381 21 6413 833; e-mail: nada.hladni@ifvcns.ns.ac.rs

                                                                                                  

 

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Published in „GENETIKA“ Vol. 47, No.3 (2015), pp. 819 -832

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

 

UDC 575

DOI: 10.2298/GENSR1503819C

                                Original scientific paper

 

 

 

 

 

CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BOSNIAN ARTISANAL DRY FERMENTED SAUSAGE (SUDŽUK) DURING FERMENTATION

 

Josip čolo¹, Sanja mihajlović², Maja tolinački², Mersiha alkić¹, Dušanka popović², Milan kojić², Amarela terzić-vidojević^2*

 

¹Faculty of Agriculture and Food Science, University of Sarajevo,

Sarajevo, Bosnia and Herzegovina

²Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,

Belgrade, Serbia

 

Abstract

Čolo J, S.Mihajlović, M.Tolinački, M. Alkić, D. Popović, M.Kojić, A.Tterzić-Vidojević (2015): Characterization of lactic acid bacteria isolated from Bosnian artisanal dry fermented sausage (sudžuk) during fermentation. - Genetika, Vol 47, No. 3, 819-832.

Bosnian sudžuk is a dry fermented sausage produced in a rural household near the town of Visoko in central Bosnia and Herzegovina. This kind of sausage was manufactured only from beef and spices in a traditional way without the addition of a starter cultures. To identify lactic acid bacteria (LAB), a total number of 160 LAB strains were isolated from five samples of Bosnian sudžuk collected over 28 days of fermentation. Preliminary identification by phenotypic tests and 16S rDNA sequencing were performed for all 160 of the LAB isolates. Identification of LAB strains from traditionally produced Bosnian sausage at the species level revealed the presence of six genera: Lactococcus sp., Enterococcus sp., Leuconostoc sp., Lactobacillus sp., Pediococcus sp. and Weissella sp.. Among the 15 distinct species identified, the species Lactobacillus plantarum, Leuconostoc mesenteroides, Lactococcus lactis, Enterococcus faecalis and Enterococcus durans were present throughout the entire process of fermentation. Leuconostoc mesenteroides, Lactobacillus plantarum and Lactococcus lactis prevailed, with 21.8%, 19.3% and 13.1%, respectively, of total LAB strains during the entire fermentation process. Significant negative correlations (r = 0.892 and r = 0.829, respectively) between the presence of Weissella sp. and Lactobacillus sp., and between the presence of Weissella sp. and Lactococcus sp. were recorded. Lactobacillus plantarum, Enterococcus durans and Leuconostoc mesenteroides were the best producers of aromogenic compounds while 32.3% of Lactobacillus plantarum and 28.6% of Leuconostoc mesenteroides were produced exopolysaccharides.

Key words: Bosnian sudžuk, fermented sausages, lactic acid bacteria, 16S rDNA sequencing

 

Corresponding author: Amarela Terzić-Vidojević,Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Vojvode Stepe 444a, P.O.Box 23, 11010 Belgrade, Serbia. Telephone: +381 11 397 59 60; Fax: +381 11 397 58 08; E-mail: amarela@imgge.bg.ac.rs; lab6@imgge.bg.ac.rs,http://www.imgge.bg.ac.rs

 

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Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 833 -838

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575

 DOI: 10.2298/GENSR1503833P

                                 Original scientific paper

 

 

 

FETAL ULTRASOUND FINDINGS IN TRISOMY 18 AT MIDPREGNANCY

 

Bojana petrović¹, Aleksandar ljubić², Milica komnenić^1, Violeta joksimović³,

Olivera lekić miladinović¹

 

¹Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade

²MEDIGROUP General hospital

³High Medical School of Professional Studies "M. Milankovic", Belgrade

 

 

Abstract

Petrović B., A. Ljubić, M. Komnenić, V. Joksimović, O. Lekić Miladinović (2015): Fetal ultrasound in trisomy18 AT midpregnacy - Genetika, Vol 47, No. 3,833 -838.

Trisomy 18 (Edwards' syndrome), a lethal chromosomal aberration, is the second most common autosomal trisomy with an incidence 1: 8000. The aim of this study is to evaluate the sonographic findings in fetuses with trisomy 18. In ten years period (2002-2012) we analyzed fetal blood samples for chromosome abnormalities. Samples were taken by cordocentesis and processed using standard techniques. Sixteen metaphase cells were analyzed for chromosomal constitution in each sample after tripsin-Giemsa banding. A retrospective review of the cytogenetic laboratory database identified all cases of trisomy 18 in ten years period. The prenatal sonographic studies in fetuses at 16 to 22 weeks' gestation, done before invasive testing for the karyotype were reviewed for anatomic findings. From 2100 samples of fetal blood analyzed for chromosomal abnormalities, there were 16 (0,8%) with complete trisomy 18. We found no mosaicism, or partial trisomy 18. The women that carried fetuses with trisomy 18 were 17 to 42 years of age. Four of them were above 35. From 16 fetuses with trisomy 18, 14 (87,5%) had some anomaly detected by ultrasound, and other two were tested because of advanced maternal age. The most common findings in trisomy 18 were intrauterine growth retardation, polyhidramnios and anomalies of central nervous system, in 29% respectively. Multiple anomalies, including central nervous system, hart and gastrointestinal system anomalies, were also frequent (21%). Therapeutic termination of pregnancy was done in all cases after genetic counseling. Screening for chromosomal abnormalities using ultrasound is at utmost importance in cases of nonhereditary aberrations. Detailed ultrasonographic examinations of fetuses will enable health care providers to form the appropriate management plan for each patient

Key words: trisomy 18, fetal ultrasound, cordocentesis

 

Corresponding author: Bojana Petrović, Bulevar Arsenija Čarnojevića 25/23,11070 Novi Beograd, tel: 0113116810 (home), 0646178320 (mobile), e-mail: bojana.petrovic1977@gmail.com

 

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Published in „GENETIKA“ Vol. 47, No.3 (2015), pp.839 -848

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575.630.11

DOI: 10.2298/GENSR1503839S

                                Original scientific paper

 

 

 

Gliadin and Glutenin polymorphism in durum wheat landraces and breeding varieties of Azerbaijan

Hamlet Baykishi sadigov

Genetic Resources Institute of ANAS, Baku, Azerbaijan

Abstract

Sadigov H. B. (2015): Gliadin and glutenin polymorphism in durum wheat landraces and breeding varieties of Azerbaijan- Genetika, Vol 47, No. 3, 839-848.

Durum wheat genotypes including 7 landraces and 17 breeding varieties were studied. Polyacrylamide gel electrophoresis under acidic conditions of pH 3.1 was used to study gliadin and glutenin polymorphisms. In total, 32 gliadin and 8 high molecular weight glutenin alleles were identi­fied. The contribution of B genome (58.5%) to the allelic variation of durum wheat varieties was higher than of A genome. The cluster analysis delineated genotypes into four main clusters. According to cluster analysis, legitimacy identifying the distribution of botanical varieties through the tree was observed. The study confirms the suitability of biochemical markers for cultivar identification and genetic relation study in durum wheat  genotypes.

Key words: stock proteins, durum wheat, landrace, electrophoresis, botanical variety

 

 

Corresponding author: Hamlet Baykishi sadigov,Genetic Resources Institute of ANAS, Avadlig ave. 155, AZE 1106, Baku, Azerbaijan,E-mail: hamlet.sadiqov@yahoo.com

 

 

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Published in „GENETIKA“ Vol. 47, No.3 (2015), pp. 849-861

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575:630

                            DOI: 10.2298/GENSR1503849G

                                 Original scientific paper

 

 

Genetic differentiation of Norway spruce (Picea abies (L.) Karst.) trees with diferent crown types from the mountain Golija

 

Vladislava galović¹, Mirjana šijačić-nikolić², Robert šafhauzer², Dijana čortan², Saša orlović¹

 

¹University of Novi Sad, Institute of Lowland Forestry and Environment

²University of Belgrade, Faculty of Forestry, Belgrade, Serbia

 

Abstract

Galović V., M. Šijačić-Nikolić, R.Šafhauzer, D. Čortan, S. Orlović (2015): Genetic differantion of Norway spruce (Picea abies (L)Karst.) trees with differnet crown types from the montain Golia - Genetika, Vol 47, No. 3,849 -861.

The knowledge of genetic diversity degree of given species is of great importance for the successful process of breeding and genetic conservation. The aim of conducted research was to determine the genetic differentiation of Norway spruce (Picea abies (L.) Karst) genotypes with very specific narrow pyramidal and normal crown type, which grows at different altitude of the mountain Golija. For assessment of genetic similarities or differences between studied genotypes co-dominant microsatellite system had been used. This system has proven to be reliable and efficient in the genetic characterization of plant species. In total 22 primer sets have been tested, while 16 (73%) of them resulted in the successful yield of the amplified product. The analysis show that studied individuals had in total 130 alleles, in average 8.125 polymorphic alleles per each locus. The lowest polymorphism was detected in the locus EATC1D10, EATC1F03B and EATC2G09, while the highest level of polymorphism was detected in EATC2G08. Based on microsatellite date and similarity matrix, cluster analysis dendrogram indicates existence of the vertical differentiation of studied genotypes, which is consistent with results of previous Norway spruce studies.

Key words: Picea abies (L.) Karst, microsatellite, genetic differentiation, Golija

 

Corresponding author: Dijana Čortan, Kneza Višeslava 1, 11000 Belgrade,011-305-3990,E-mail: dijanacortan@yahoo.com

 

 

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Published in „GENETIKA“ Vol. 47, No.3 (2015), pp. 863-876

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575

                            DOI: 10.2298/GENSR1503863S

                                Original scientific paper

 

 

SEGMENTATION AND ABNORMALITY DETECTION OF CERVICAL CANCER CELLS USING FAST ELM WITH PARTICLE SWARM OPTIMIZATION

 

P.SUKUMAR^1* and R.K.GNANAMURTHY²

 

¹ Faculty of Electronics and Communication Engineering,

Nandha Engineering College, Erode, India.

²Principal, SKP Engineering College, Tiruvannamalai, India

 

 

Abstract

Sukumar P. and R.K.Gnanamurthy (2015): Segmentation and abnormality detection of cervical cancer cells using fast elm with particle swarm optimization.- Genetika, Vol 47, No. 3,863-876.

Cervical cancer arises when the anomalous cells on the cervix mature unmanageable obviously in the renovation sector. The most probably used methods to detect abnormal cervical cells are the routine and there is no difference between the abnormal and normal nuclei. So that the abnormal nuclei found are brown in color while normal nuclei are blue in color. The spread or cells are examined and the image denoising is performed based on the Iterative Decision Based Algorithm. Image Segmentation is the method of paneling a digital image into compound sections. The major utilize of segmentation is to abridge or modify the demonstration of an image. The images are segmented by applying anisotropic diffusion on the Denoised image. Image can be enhanced using dark stretching to increase the quality of the image. It separates the cells into all nuclei region and abnormal nuclei region. The abnormal nuclei regions are further classified into touching and non-touching regions and touching regions undergoes feature selection process. The existing Support Vector Machines (SVM) is classified few nuclei regions but the time to taken for execution is high. The abnormality detected from the image is calculated as 45% from the total abnormal nuclei. Thus the proposed method of Fast Particle Swarm Optimization with Extreme Learning Machines (Fast PSO-ELM) to classify all nuclei regions further into touching region and separated region. The iterative method for to training the ELM and make it more efficient than the SVM method. In experimental result, the proposed method of Fast PSO-ELM may shows the accuracy as above 90% and execution time is calculated based on the abnormality (ratio of abnormal nuclei regions to all nuclei regions) image. Therefore, Fast PSO-ELM helps to detect the cervical cancer cells with maximum accuracy.

Key words: Cervical Cancer, Image Denoising, Extreme Learning Machine, White Blood Cells, Particle Swarm Optimization, Fast Extreme Learning Machine.

 

Corresponding author: P.sukumar, Faculty of Electronics and Communication Engineering, Nandha Engineering College, Erode, India, sukumarnandha@gmail.com

 

 

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Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 877 - 884

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

                            DOI: 10.2298/GENSR1503877B

                                Original scientific paper

 

 

From clinical suspect to molecular confirmation

of Noonan syndrome; contribution of “best practice” genetic counseling and new technical possibilities

 

 

Nenad BUKVIC^1, , Dora VARVARA², Cesare ROSSI³, Maria Felicia FAIENZA⁴,

Francesco C. SUSCA², Nicoletta RESTA²

 

¹ Azienda Ospedaliero Universitaria Consorziale, Policlinico di Bari, UOC  Laboratorio di Genetica Medica. Bari, Italia.

² UOC Laboratorio di Genetica Medica – Dipartimento di Scienze Biomediche ed Oncologia Umana - Università degli Studi di Bari “A Moro”, Bari,  Italia

³ U.O. Laboratorio di Genetica Medica- A.O.U. di Bologna, Bologna, Italia

⁴ Lab. Biologia Molecolare- Endocrinologia e Diabetologia Pediatrica- Dipartimento di Biomedicina dell’Età Evolutiva- Università degli Studi di Bari “A Moro”, Bari,  Italia

 

Abstract

Bukvic N., D. Varvara, C. Rossi, M. Felicia Faienza, F. C. Susca, N. Resta (2015): From clinical suspect to molecular confirmation of noonan syndrome; contribution of “best practice” genetic counseling and new technical possibilities- Genetika, Vol 47, No. 3, 877-884.

Noonan syndrome (NS) is an autosomal dominant disorder, characterized by variable expressivity of clinical features such as: postnatal growth reduction, congenital heart disease, characteristic facial dysmorphisms and development delay. In ~75% of all NS cases, germline mutations involving RAS-MAPK signaling pathway genes (PTPN11, SOS1, RAF1, KRAS, NRAS, BRAF, SHOC2, MEK1, CBL) are causative. 

We reported a case of 13-year-old girl [born at 36w by CS (BW 3250 g (~95°), BL 48 cm (~75°)] referred for genetic counseling due to growth retardation, facial dysmorphisms, development delay and learning disability. After birth she presented frequent vomiting, with failure to thrive and at 5 months of age underwent surgery for intestinal malrotation. Because of short stature, Growth Hormone (GH) therapy have been introduced at age of 3yrs up to 11yrs. Negative molecular testing for PTPN11 and SOS1 genes, normal female karyotype and aCGH analysis were observed. 

Objective examination: H 138 cm, (<3°); W 33 kg, (<3°), no menarche, hypertelorism, eyelids ptosis with down slanting palpebral fissures, low-set and posteriorly rotated ears, high-arched palate, micrognathia, short and webbed neck, low hairline at the back of the neck, pectus excavatum, prominent scoliosis, joint hyperextensibility,  bilateral pes planus and mitral valve prolapse disclosed by US.

Phenotype of our patient was suggestive to NS, thus further mutational screening has been requested. Missense mutation in exon 2 of KRAS gene (c.40G>A; p.Val14Ile) has been identified. Even though KRAS mutations are usually associated with NS severe phenotype with cardiac involvement (hypertrophic cardiomyopathy), this finding is not present in our patient.

Key words: Noonan Syndrome, RAS-MAPK signaling pathway genes, RASopathies, KRAS mutations

 

Corresponding author: Nenad Bukvic, Azienda Ospedaliero Universitaria Consorziale, Policlinico di Bari, UOC Genetica Medica. Piazza G. Cesare, 11, 70124 Bari, Italia. e-mail: nenadbukvic@virgilio.it; Tel: +39 080 5593621; Fax: +39 080 5593618

 

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Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 885- 900

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575:630

                            DOI: 10.2298/GENSR1503885G

Original scientific paper

 

 

 

EXPLORING GENETIC DIVERSITY AND QUALITY TRAITS IN A COLLECTION OF ONION (Allium cepa L) LANDRACES FROM NORTH-WEST SPAIN

 

Susana GONZÁLEZ–PÉREZ¹, Cristina MALLOR², Ana GARCÉS–CLAVER², Fuencisla MERINO¹, Alfredo TABOADA³, Antonio RIVERA³, Federico POMAR¹, Dragan PEROVIC^4,5, Cristina SILVAR^1*

 

¹ Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Universidade da Coruña, Campus da Zapateira,, A. Coruña, Spain

² Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza, Spain

³ Centro de Investigaciones Agrarias de Mabegondo, A  Coruña, Spain

⁴ Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany

⁵ EDUCONS University, Svilajnac, Serbia

 

 

Abstract

González–Pérez S., C. Mallor, A. Garcés–Claver, F. Merino, A. Taboada, A. Rivera, F Pomar, D. Perovic, C. Silvar (2015): Exploring genetic diversity and quality traits in a collection of onion (Allium cepa l) landraces from north-west Spain- Genetika, Vol 47, No. 3, 885-900.

Seventeen onion landraces from North-West Spain were evaluated using microsatellites markers. Eleven polymorphic markers identified 32 alleles in the whole collection, with an average of 2.9 alleles per locus. High values of observed (mean of 0.45) and expected heterozigosity (mean of 0.51) were detected for the majority of loci. Wright’s fixation index confirmed an excess of heterozygotes and a low level of inbreeding within the collection. Multivariate analyses revealed that Oimbra was the most distinctive genotype. The remaining 16 onion genotypes were in part assorted according to some morphological traits of bulbs. Pungency and solid soluble content highly varied among landraces and bulbs. Five landraces were classified as sweet, whereas 9 possessed medium pungency and 3 were recorded as pungent. This onion collection represents a useful source of genetic heterogeneity that might be exploited in breeding programs for the generation of new onion varieties that satisfy consumer demands.

            Key words: onion, Allium cepa, landrace, genetic diversity, pyruvic acid, solid soluble content

 

Corresponding author: Cristina Silvar, Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Universidade da Coruña, Campus da Zapateira s/n, E–15071, A  Coruña, Spain, e-mail: c.silvar@udc.es,Tel.: + 34 881012262

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Published in „GENETIKA“ Vol. 47, No.3 (2015), pp. 901 -908

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575

                             DOI: 10.2298/GENSR1503901D

    Original scientific paper

 

 

 

VERVIEW OF EUROPEAN POPULATION CLUSTERING BASED ON 23 Y-STR LOCI

 

Serkan dogan^1*, Adna ašić¹, Sanida buljubašić¹, Larisa bešić¹, Monia avdić¹, Elma ferić¹, Mirsada hukić¹, Yusuf turan¹ and Damir marjanović¹

 

¹Department of Genetics and Bioengineering, Faculty of Engineering and IT, International Burch University, Sarajevo, Bosnia and Herzegovina

 

Abstract

 

Dogan S., A. Ašić, S. Buljubašić, L. Bešić, M. Avdić, E.Ferić, M. Hukić, Y. Turan and D. Marjanović (2015): Overview of European population clustering based on 23 y-str loci, Genetika, Vol 47, No. 3,901-908.

Short tandem repeats (STRs) located on the Y-chromosome are a useful tool for various scientific fields, such as forensic investigation, but also for the investigation of population structure and molecular history. In this study, population data based on 23 Y-STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) from 23 European human populations were compared. All haplotype data for this research were gathered from previously published articles. Arlequin v3.5.1.2, POPTREE2, and MEGA 5.1 software packages were used for the calculation of allelic frequencies and genetic distance, and the construction of the European, as well as worldwide phylogenetic trees. Obtained results indicate a formation of several distinct sub-clusters within European population cluster. Observed sub-clusters were mostly recognized within geographically closer populations, meaning that neighboring populations were a part of the same sub-cluster in most of the cases. Compared with the previously published results obtained using autosomal STR markers, a significant level of concordance was detected. However, it seems that Y-STRs analyzed in this study are more informative since they enabled regional clustering in addition to continental clustering. Also, the use of a larger number of loci yielded clustering that is more specific than what has been calculated to date. Finally, it can be concluded that this study has shown that the application of a larger number of loci enables the more detailed insight into the relationships between European populations, compared to what has been published before.

Key words: Y-chromosome, Y-STRs, PowerPlex Y23, European populations, population clustering, phylogenetic tree, population genetics

 

Corresponding author: Serkan Dogan, International Burch University, Francuske revolucije bb, Ilidža, 71210 Sarajevo, Bosnia and Herzegovina. Tel: +387 33 944 400 Fax: +387 33 944 500 E-mail: serkan.dogan@ibu.edu.ba

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Published in „GENETIKA“ Vol. 47, No. 3 (2015), pp. 909-916

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575

                             DOI: 10.2298/GENSR1503909O

                                   Original scientific paper

 

 

MITOCHONDRIAL COI IN PHYLOGENETIC RELATIONSHIPS OF LAIMAPHELENCHUS BELGRADIENSIS (NEMATODA: APHELENCHOIDIDAE)

Violeta ORO, Nenad MILOVANOVIĆ, Violeta PETROVIĆ, Bogdan NIKOLIĆ,

Jovana BLAGOJEVIĆ

 

Institute of Plant Protection and Environment, Belgrade, Serbia

 

Abstract

Oro V., N. Milovanović, V. Petrović, B. Nikolić, J. Blagojević (2015): Mitochondrial COI in phylogenetic relationships of Laimaphelenchus belgradiensis (nematoda: Aphelenchoididae).- Genetika, Vol 47, No. 3,909 -916.

Nematodes of the genus Laimaphelenchus are small and tiny organisms. Some parts of their body are measured in nanometers. The identification and classification of such organisms is a complex task. Previously, the major source of classification was morphology based on anatomical characters and measurements. Nowadays, this approach is supplemented by: “nano-morphology” based on scanning electron microscopy and molecular data and phylogeny, resulting in molecular systematics. Laimaphelenchus belgradiensis was recently described species. Since cytochrome c oxidase subunit I gene was successful in DNA based species diagnosis, it was chosen as a molecular marker to infer phylogeny of the newly discovered species. Phylogenetic relationships were based on Bayesian inference, the pairwise distances and the content of nitrogenous bases. The great genetic diversity was observed among close and distant species.

Key words: Laimaphelenchus belgradiensis, mCOI, phylogeny

 

Corresponding author: Violeta Oro, Institute of Plant Protection and Environment, Teodora Drajzera 9,  Belgrade, Serbia, Phone: 381 11 2660 049, e-mail: viooro@yahoo.com

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 917- 926

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575

                                              DOI: 10.2298/GENSR1503917S

Original scientific paper

 

 

DEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS

Puccinia triticina

 

Hülya SIPAHI^1*, Ayşen YUMURTACI², Zafer MERT³

 

 

University of Sinop, Faculty of the Arts and Sciences, Department of Biology, Sinop, Turkey.

²Marmara University, Faculty of Science and Letters, Department of Biology, , Istanbul, Turkey

³Central Research Institute for Field Crops, Department of Plant Pathology, Ankara, Turkey.

 

 

Abstract

 

Sipahi H., A. Yumurtaci, Z.R Mert (2015): Development of novel markers, using computationally extracted classi type EST-SSRS, in wheat leaf rust fungus Puccinia triticina.- Genetika, Vol 47, No. 3, 917-926.

This study focused on the development of EST-simple sequence repeats markers and the detection of their transferability and their utility for evaluating wheat leaf rust pathogen diversity. A total of 44,407 publicly available EST sequences derived from Puccinia triticina were computationally mined. Di-nucleotide repeat density covered the vast majority of assembled ESTs (45%). The tri-repeat motif (TCT) and penta-repeat motif (TTCTT) were the most repeated motif. A set of 103 Class I type sequences containing simple sequence repeats were further analyzed by BLASTX similarity. Nineteen primer pairs flanking regions of EST-SSRs were designed. Of the 19 primer pairs tested, 10 successfully amplified fragments. Their polymorphism was evaluated with 8 Puccinia triticina (Pt) single-uredinal isolates collected from the different regions of Turkey. These newly developed EST-SSR primer pairs can be implicated as stable markers for pathogen diversity analysis. It was also shown that some leaf rust EST-SSR markers were capable of cross-amplification in P. graminis f. sp. tritici.

Key words: Puccinia triticina, EST, simple sequence repeats

 

Corresponding author: Hülya Sipahi, University of Sinop, Faculty of the Arts and Sciences, Department of Biology, Sinop, Turkey,Telephone: 90 368 2715516/4206, e-mail: hulyasipahi@gmail.com

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 927- 939

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575

                           DOI: 10.2298/GENSR1503927V

Original scientific paper

 

 

 

CYTOGENETIC ABNORMALITIES IN LYMPHOCYTES EVALUATED WITH MICRONUCLEUS ASSAY IN MEDICAL PERSONNEL OCCUPATIONALLY EXPOSED TO IONIZING RADIATION

 

Nevenka VELICKOVA, Misko MILEV, Tatjana RUSKOVSKA, Biljana PETROVA,

Bojana NEDELJKOVIK, Pale GORGIEVA

 

Faculty of medical science, University “Goce Delcev” - Stip,R. of Macedonia

 

Abstract

Velickova N., M. Milev, T. Ruskovska, B. Petrova, B. Nedeljkovik, P. Gorgieva (2015): Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation.--Genetika, Vol 47, No. 3, 927-939.

The aim of this study was to evaluate the genotoxicity of ionizing radiation on medical personnel using the micronucleus assay and to determine the human health risk. Paired Student’s t-test shows significant statistical difference between the total number of binucleated (BN) cells with micronuclei within the two groups (exposed and control) (t=6,812; p<0,05). The mean of MN frequencies in the exposed group increased in comparison with the mean of MN frequencies in the control group. The formation of small and large micronuclei indicates that medical personnel who are exposed on radiation in their work place, have a chromosomal instability and a risk of cancer.

Key words: micronucleus, lymphocytes, genotoxicity, ionizing radiation, cancer

 

Corresponding author: Nevenka Velickova, Faculty of medical science, University “ Goce Delcev” - Krste Misirkov 10-A, 2000 –Stip, R.Macedonia, nevenka.velickova@ugd.edu.mk

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 941-950

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575:633

                             DOI: 10.2298/GENSR1503941D

    Original scientific paper

 

 

MAIZE NUTRIENT UPTAKE AFFECTED BY GENOTYPE AND FERTILIZATION

Ivica đalović¹, Đorđe jocković¹, Yinglong chen², Goran bekavac¹,

Srđan šeremešić³, Goran jaćimović³, Milka brdar–jokanović¹

 

¹Institute of Field and Vegetable Crops Novi Sad, Novi Sad, Serbia

²School of Earth and Environment and Institute of Agriculture, University of Western Australia Australia

³University of Novi Sad, Faculty of Agriculture, Department of Field and Vegetable Crops, Novi Sad, Serbia

 

Abstract

Đalović I., Đ. Jocković, y. Chen, G. Bekavac, S.Šeremešić, G. Jaćimović, M. Brdar–Jokanović  (2015): Maize nutrient uptake affected by genotype and fertilization.-Genetika, Vol 47, No. 3,941- 950.

The content of nutrients in maize are commonly related with fertilization and soil quality and rarely explained with the individual hybrid properties. Therefore, the aim of this study is to access a long term fertilization system on ear leaf of Mg, Fe, Mn and Cu content in six maize hybrids(NS 3014, NS 4015, NS 5043, NS 6010, NS 6030 and NS 7020). Samples were collected from a long-term experiment at the Rimski Šančevi experimental field of the Institute of Field and Vegetable Crops in Novi Sad. The study included maize monoculture and 2-year rotations with the application of NPK and manure. Results showed that ear Mg content was influenced with the treatments, hybrid and their interaction and ranged from 1.77-2.69 g kg^-1. Iron variability was significantly affected with the treatments and interaction (hybrid x treatments) in range from 103.2 to151.9g kg^-1. The ear manganese content (41.1-63.6g kg^-1) derived from treatments and hybrid effect and Cu (12.3-23.6 g kg^-1) was significantly influenced with treatments. Across all treatments, in average, NS6030 had higher values of nutrient and NS3014 was lower in ear nutrient content. This indicates that vegetation length could favor nutrient accumulation. Obtained results suggested that even on fairly productive soil such as Chernozem hybrid selection and the balanced fertilization is crucial for managing the maize nutrient content.

Key words: genotype, fertilization, micronutrients, maize, cropping system.

 

Corresponding author: Ivica đalović, Institute of Field and Vegetable Crops Novi Sad, 21000 Novi Sad, MaksimaGorkog 30, 21000 Novi Sad, Serbia

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp.951-958

© 2015 Serbian Genetics Society

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

 

UDC 575:630

                             DOI: 10.2298/GENSR1503951G

Original scientific paper

 

 

MINERAL CONTENT IN A SALAD LEAF (Lactuca sativa l.)  DEPENDING OF THE GENOTYPE AND APPLIED AGRICULTURAL MEASURES

 

Aleksandra govedarica-lučić and Goran perković

 

University in East Sarajevo, Faculty of Agriculture, East Sarajevo, Bosnia and Herzegovina

 

Abstract

Govedarica-Lučić A. and, G. Perković (2015): Mineral content in a salad leaf (Lactuca sativa L.)  depending of the genotype and applied agricultural measures-Genetika, Vol 47, No. 3,951 -958.

Four different production methods applied on three genotype of lettuce (Archimedes RZ, Santoro RZ, Kibou RZ) – have been estimated in a two-year experiment carried out at the Agricultural University of East Sarajevo. The experiment has been conducted during two-year period; lettuce was cultivated by using four different mulch materials: black PE foil, agro textile, a combination of PE foil and agro textile; control variant (bare soil). After harvesting lettuce, evaluated the content of the following minerals (N, P, K). The values of nitrogen content tend to increase from bare ground (7.47%) to a covered soil (7.98%). The reaction genotype on variants coverage shows an interaction effect. Type of material for covering soil also has an influence on the content of potassium. The highest potassium content of 4.87% was recorded at the variant of covering with agro textile, or by 18% more in comparison to the control variant.

Key words: genotype, agricultural technology , minerals

 

Corresponding author: Aleksandra Govedarica-Lučić,University of East Sarajevo, Faculty of Agriculture, sandraklepic@yahoo.com;+38757342-701

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 959-966

© 2015 Serbian Genetics Society

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575

                            DOI: 10.2298/GENSR1503959S

Original scientific paper

 

 

 

FOUR DECADES OF INVERSION POLYMORPHISM IN Drosophila pseudoobscura FROM MEXICO

 

Victor M. salceda¹, Judith guzmán-rincón², Ma  esther de la rosa³ and Olga olvera³

 

¹Departamento de Biología, Instituto Nacional de Investigaciones Nucleares,

²Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México. México.  ³Retired.

 

 

Abstract

         Salceda M.. V, J. Guzmán-Rincón, M.  Esther de la Rosa and O. Olvera  (2015): Four decades of inversion polymorphism in Drosophila pseudoobscura from Mexico- Genetika, Vol 47, No. 3, 959-966.

         Chromosomal inversion polymorphism has been study in different natural and experimental populations of Drosophila species. D pseudoobscura first studies in Mexico started during the late 30´s of last century, here are shown the relative frequencies of 48 populations in a period of 40 years with a grand total of 7266 third chromosomes analyzed. Globally in those populations a total of 26 inversions are present, in most cases their genetic structure is built by five main gene arrangements and three to six in minor frequencies not reaching a particular one more than six percent. This study includes the description of 15 new inversions reported else were. The most frequent gene arrangements found were TL, CU, SC, EP and OA that globally represent about the 90% of the whole sample. Remaining 21 inversions were found with individual frequencies varying from one to six per cent reaching in general the remaining 10%. In some areas the dominant inversions are CH. AR. ST and PP. in some areas. Important goals   of this study are the discovery and description of 15 new gene arrangements as well  new species description, D. cuauhtemoci.  All of them reported else were.

Key words: Drosophila pseudoobscura, natural populations, inversion polymorphism

 

Corresponding author: Victor M. salceda, Departamento de Biología, Instituto Nacional de Investigaciones Nucleares, victor.salceda@inin.gob.mx.

 

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 967-982

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575

                           DOI: 10.2298/GENSR1503967P

Original scientific paper

 

 

 

THE IMPACT OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELD

 (50 HZ, 0.25 MT) ON FITNESS COMPONENTS AND WING TRAITS OF Drosophila subobscura

 

Aleksandra patenković¹, Tatjana savić¹, Bojan kenig¹,

Zorana kurbalija novičić¹, Marko anđelković²

 

¹University of Belgrade - Institute for Biological Research “Siniša Stanković”, Department of Genetics of Populations and Ecogenotoxicology, Belgrade, Serbia

²Serbian Academy of Sciences and Arts, Belgrade, Serbia

 

Abstract

 

Patenković A., T. Savić, B. Kenig, Z. Kurbalija Novičić, M. Anđelković (2015): The impact of extremely low frequency electromagnetic field (50 hz, 0.25 mt) on fitness components and wing traits of Drosophila subobscura,  Genetika, Vol 47, No. 3, 967-982

Over the past century, man-made electromagnetic fields (EMF) have changed the natural environment, representing one of the most common and rapidly increasing environmental factors as technology advances. Numerous studies of biological effects of exposure to EMF have been performed on Drosophila during the last 30 years, showing contrasting results. In an attempt to determine whether EMF exposures might affect the development of Drosophila, the present study examines phenotypic variations through direction and range of changes in several fitness components and wing length and width, of Drosophila subobscura isofemale lines, affected by 50 Hz EMF. Embryonal and early post-embryonal stages were exposed to homogeneous sinusoidal 0.25 mT EMF of 50 Hz. The EMF exposure caused a significantly prolonged developmental time and significantly increased the wing size with the opposite direction of directional asymmetry compared to the control group. These results shows that even such a weak EMF has the potential to modulate, through developmental pathways, some of the fitness-related traits, wing size, and wing asymmetry in D. subobscura even after one generation of exposure, showing that extremely low frequency (ELF) EMFs have relevant consequences on development, adult morphology, and fitness.

Key words: Drosophila subobscura, electromagnetic fields (EMF), fitness components, wing size, directional asymmetry

 

Corresponding author: Aleksandra Patenković, Department of Genetics of Populations and Ecogenotoxicology, Institute of Biological Research “Siniša Stanković”, University of Belgrade, Bul. despota Stefana 142, 11060 Belgrade, Serbia;Phone number: +381 11 20 78 328; Fax number: +381 11 27 61 433;E-mail: aleksandra@ibiss.bg.ac.rs

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 983-992

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575.633.11

                            DOI: 10.2298/GENSR1503983B

Original scientific paper

 

 

 

 

APPLICATION OF WHEAT SSR MARKERS FOR DETECTION OF GENETIC DIVERSITY IN TRITICALE (X Triticosecale Witt. )

 

Želmíra balážová^*, Andrej trebichalský, Zdenka gálová,
Radomíra hornyák gregáňová

 

 Slovak University of Agriculture in Nitra, Nitra, Slovak Republik

Abstract

 

Balážová Ž., A. Trebichalský, Z. Gálová, R. Hornyák Gregáňová (2015): Application of wheat SSR markers for detection of genetic diversity in triticale (x Triticosecale witt.- Genetika, Vol 47, No. 3,983 -992.

Present study aims to testify usefulness of particular wheat SSR markers for the detection of genetic diversity degree in the set of 59 triticale cultivars and new lines coming from different European countries and USA. For this purpose, a set of fifteen SSR markers were used. One SSR marker (Xwmc429) gave a uniform spectrum. The set of fourteen polymorphic markers provided 94 alleles with an average frequency of 6.71 alleles per locus. The number of alleles ranged between 2 (Xbarc 195) and 10 (Xbarc 137). Resulting from the number and frequency of alleles, diversity index (DI), polymorphic information content (PIC) and probabilities of identity (PI) were calculated. An average value of PIC for 14 markers was 0.640, the highest value was calculated for wheat SSR marker Xgwm 46 (0.809). Based on UPGMA algorithm, a dendrogram was constructed. It was able to separate 57 of 59 cultivars (96,6 %) from each other. American new-line NE-422T significantly separated from all cultivars and new lines. Only two french cultivars Bienvenu and Wilfried had not been separated from each other. A tested set of SSR markers allowed to better understand genetic relationships among European cultivars and American  new lines. In general, a dendrogram along with results of calculated genetic indicators such as PIC, PI and DI point out at SSRs markers as high informative and usefull tool in genetic diversity research between close-related species.     

Key words: triticale, microsatellites, polymorphism, dendrogram        

 

Corresponding author: Želmíra Balážová, Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, Nitra, phone: +421376414327, e-mail: zelmira.balazova@uniag.sk

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 993-1011

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

 

UDC 575.630

                           DOI: 10.2298/GENSR1503993D

Original scientific paper

 

 

INVESTIGATION OF WILD SPECIES POTENTIAL TO INCREASE GENETIC DIVERSITY USEFUL FOR APPLE BREEDING

 

Catalina dan¹, Adriana F. sestras^1,*, Calin bozdog^1,2, Radu E. sestras¹

 

¹University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

²Fruit Research Station, Cluj-Napoca, Romania

 

Abstract

Dan C., A. F. Sestras, C. Bozdog, R.E. Sestras (2015): Investigation of wild species potential to increase genetic diversity useful for apple breeding - Genetika, Vol 47, No. 3, 993-1011.

The potential of testing new apple cultivars and the possibility to induce valuable traits is directly dependent on the availability of sufficient genetic diversity, while apple breeding has narrowed the genetic ground of commercial cultivars. Wild species were studied in regard to their influence upon progenies and their capacity to enlarge apple genetic diversity. The interspecific seedlings were framed in five biparental mating (paired crosses), in which Malus species were crossed with different cultivars, obtaining half-sib families. The number of F₁ progenies per combination varied from 31 (Cluj 218/2 × M. floribunda) up to 142 (Reinette Baumann × M. floribunda), with a total of 1650 hybrids F₁. The influences upon vigour and juvenile period and possible correlation among fruit size and taste were analyzed. Juvenile period varied from 6.00 (M. zumi × Jonathan) to 9.31 years (Cluj 218/2 × M. floribunda). Data based on correlation coefficient illustrated that the fructification year was not influenced by the vigour of trees. The highest value of correlation for fruit’s size and taste was obtained among M. coronaria hybrids. This result might suggest that once the fruit are larger, there is a high chance the taste is also more appreciative and fruit quality for mouth feels increase. Depending on the parental formula, additive effects may be inferior compared to genetic effects of dominance and epistasis. Although M. zumi and M. floribunda achieved the same genetic gain (0.31), M. zumi had a higher expected selection response for fruit size. The difficulty of obtaining seedlings with tasty and large fruit when wild Malus species are used as genitors is resulting from the values of expected selection response data, but in the same time results confirm that wild Malus species are suitable resources for genetic variability, both for dessert and ornamental apple cultivars.

Key words: crabapple, expected selection response, genetic gain, genotype, heritability, hybridization, Malus wild species, seedlings

 

Corresponding author: Adriana F. Sestras,Faculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Manastur St., 400372 Cluj-Napoca, Romania, Phone 04+264-596384 int.176, Fax 04+264-593792, e-mail adriana.sestras@usamvcluj.ro.

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 1013-1019

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633

                                 DOI: 10.2298/GENSR1503013D

Original scientific paper

 

 

COMPARATIVE STUDY OF I/D POLYMORPHISM OF ACE GENE IN DIABETES TYPE-2 PATIENTS AND CONTROL GROUP IN UNRELATED GUJARATI POPULATION

 

Darshan D. doshi¹, Druti B. shah¹, Krishna M. singh² and Rajesh K. patel³*

 

¹ Genetics Department, Ashok and Rita Patel Institute of Integrated study and Research in
  Biotechnology and Allied Sciences (ARIBAS), New Vallabh Vidyanagar- 388 121 India

²  Xcelris Labs Limited, Ahmedabad-380 054, India.(present address)

³ Sandor Animal Biogenics Pvt Ltd., Hyderabad- 500034 India (present address)

.

 

Abstract

Doshi D. D, D. B. Shah, K. M. Singh and R. K. Patel (2015): Comparative study of I/D polymorphism of Ace gene in diabetes type-2 patients and control group in unrelated Gujarati population- Genetika, Vol 47, No. 3, 1013-1019.

Many of the previous studies indicated that the I/D polymorphism of ACE gene is associated with diabetes type-2. To validate the association of I/D polymorphism in ACE gene, a study was designed in non-diabetic (normal) and diabetic type-2 patients of unrelated Gujarati population. The random blood samples from 36 normal and 36 diabetic type -2 patients of above 45 years were collected for the studies. DNA was extracted from blood samples for PCR by using ACE specific primers. The gene and genotype frequencies were estimated for different alleles observed in diabetic as well as in normal healthy persons. In present study, all three genotypes that is, I/I (477bp), I/D (477/190bp), D/D (190bp) were observed in samples from normal and diabetic patients. Among all genotypes ID (58.3%) has maximum genotypic frequency in diabetic than Non diabetic individuals (44.4%), frequency of II (27.7%) is more in Non diabetic individuals than Diabetic individuals (19.4%) and genotypic frequency of DD (27.7%) is more in Non diabetic than Diabetic individuals (22.22%). The results were not in agreement with so many previous studies. However, recent findings of other studies conducted in different ethnic groups are similar to our findings which do not support that I/D polymorphism are associated with type-2 diabetes.

Key words: I/D polymorphism, ACE gene, DNA, PCR, Genotype and gene frequencies

 

Corresponding author: Rajesh K. patel, Sandor Animal Biogenics Pvt Ltd., Hyderabad- 500034 India (present address), rkpatel46@yahoo.com

 

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 1021-1028

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

                                  DOI: 10.2298/GENSR1503021S

Original scientific paper

 

 

VARIANTS IN VDR AND NRAMP1 GENES AS SUSCEPTIBILITY FACTORS

FOR TUBERCULOSIS IN THE POPULATION OF SERBIA

 

Vesna škodrić trifunović^1,2, Ivana buha^1,2, Dragana jovanović^1,2, Violeta vučinić^1,2, Mihailo stjepanović^1,2, Vesna spasovski³, Marina andjelković³,

 Miša vreća³, Anita skakić³, Vladimir gašić³ and Sonja pavlović³*

 

¹ Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia

² School of Medicine, University of Belgrade, Belgrade, Serbia

³ Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,

Belgrade, Serbia

 

Abstract

Škodrić TrifunovićV., I. buha, D. jovanović, V. vučinić, M. stjepanović, V. spasovski, M. andjelković, M. vreća, A. skakić, V. gašić and S. pavlović (2015): Variants in VDR and NRAMP1 genes as susceptibility factors for tuberculosis in the population of Serbia-Genetika, Vol 47, No. 3,1021 -1028.

Tuberculosis (TB) is granulomatous diseases caused by Mycobacterium tuberculosis (MTB). TB is a highly infectious disease that primarily affects the lungs. One-third of human population is infected with MTB, therefore it is of utmost significance to determine the factors that influence the individual susceptibility to the disease. Host genetic factors have been recognized as essential for susceptibility to TB, since only 5% to 10% of infected individuals develop the disease. A number of candidate genes has been intensively studied, the most of which were connected with the function of macrophages, thus participating in immune response. Here we examined the gene variants of VDR (FokI) and NRAMP1 (INT4, D543N, 3’UTR) genes in aim to make the correlation between these genetic factors and risk of TB in Serbian patients. This study included 110 TB patients and 67 healthy controls. Pulmonary TB was diagnosed by clinical symptoms, radiological evidence of TB and bacteriological criteria (Culture- positive/ smear- positive). Genotyping was performed using PCR-RFLP method. Our findings revealed significant prevalence of ff genotype and variant allele f of the FokI VDR gene variant in patients compared to control group. Based on the our results the carriers of ff genotype are five times more at risk to tuberculosis than carriers of FF and Ff genotype in our population. The results of analyzed SNPs in NRAMP1 gene showed no statistically significant difference in distribution of the gene variants between patient and control groups. Therefore, we could conclude that the genotype ff of the VDR gene is factor that strongly contribute to susceptibility to TB in Serbian population.

Key words: Genetics, Pulmonary tuberculosis, Polymorphism

 

Corresponding author: Pavlovic Sonja, Laboratory for Molecular Biomedicine,Institute of Molecular Genetics and genetic Engineering, University of Belgrade,Vojvode Stepe 444a, 11010 Belgrade, Serbia,Fax: +381 11 3975 808,e-mail: sonya@sezampro.rs

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp 1029-1050

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630

                                   DOI: 10.2298/GENSR1503029V

Original scientific paper

 

 

 

 

BIOCHEMICAL CHARACTERIZATION BASED ON SDS-PAGE ANALYSIS AND CORRELATION AMONG TRAITS IN OPIUM POPPY (Papaver somniferum L.) GERMPLASM

 

Nidhi verma¹, *Sudhir shukla¹, Kusum yadav², Brij K. mishra¹ and Anu rastogi¹

 

¹Genetics and Plant Breeding, National Botanical Research Institute, Lucknow-226001, U.P., India

²Department of Biochemistry, University of Lucknow, Lucknow-226020, U.P., India

 

 

Abstract

Verma N., S. Shukla, K. Yadav, B. K. Mishra and A. Rastogi (2015): Biochemical characterization based on SDS-PAGE analysis and correlation among traits in opium poppy (Papaver somniferum L.) germplasm.- Genetika, Vol 47, No. 3,1029 -1050.

This research aimed to investigate the genetic diversity based on the pattern of seed storage protein and to establish correlation between alkaloid and seed protein content among 124 germplasm lines of opium poppy (Papaver somniferum L.). Twenty seven polypeptide bands in range of 6 to 205 kDa were recorded. Similarity index was calculated by using Jaccard’s Similarity index and cluster analysis was performed using UPGMA model. Polymorphism was observed in three variable regions i.e., high, medium and low molecular weight, among which bands of 10.4, 20, 22, 24, 30, 32, 33, 49 and 205 kDa’s were common in all the germplasms but other bands showed variation. All the 124 germplasms were broadly grouped into 13 clusters based on average linkage method. None of the trait showed significant correlation with seed protein content. The differential protein profile of the germplasms revealed wide variability and diversity among the studied germplasms which could be further used in hybridization programme to obtain maximum gain.

Key words: Alkaloids; Correlation; Opium Poppy; Path analysis; Seed storage protein

 

Corresponding author: S. Shukla, National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, U.P., India Tel: 91-522-2297936; Fax: 91-522-2205836; e-mail: s_shukla31@rediffmail.com

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 1051-1062

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia

UDC 575

                           DOI: 10.2298/GENSR1503051C

Original scientific paper

 

 

 

 

NEW DATA ON STERLET (Acipenser ruthenus L.) GENETIC DIVERSITY IN THE MIDDLE AND LOWER DANUBE SECTIONS, BASED ON MITOCHONDRIAL DNA ANALYSES

 

Gorčin CVIJANOVIĆ¹*, Tanja ADNAĐEVIĆ², Mirjana LENHARDT², Saša MARIĆ³

 

¹ Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia

² Institute for Biological Research ‘’Siniša Stanković’’, University of Belgrade, Belgrade, Serbia

³ Institute for Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

 

Abstract

Cvijanović G., T. Adnađević, M. Lenhardt, S. Marić (2015): New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses.- Genetika, Vol 47, No. 3, 1051 -1062

Poor regulated fishery, pollution, fragmentation and loss of habitat are most important factors influencing decline of sterlet population worldwide. In Middle and Lower Danube region, this species still have significant economic importance since wilde populations are commercially exploited, while Upper Danube populations are dependent on stocking efforts in order to maintain their presence in open waters. Aim of present study is to analyze genetic diversity of sterlet populations from the Middle and Lower Danube and Lower Tisza rivers, as a prerequisite for their effective conservation and management. Analysis of a highly variable D-loop fragment of mitochondrial DNA detected five new haplotypes, while the eight previously identified haplotypes had extended their previous range. Genetic variability could be attributed almost entirely to individuals, with observed lack of population structure. Negative values of neutrality test indicate recent expansion on some sampling locations. Adittionaly, gene flow analysis between Lower and Middle Danube region showed intensive exchange of speciemens. At the same time analysis showed some influence of Tisza dam on gene flow between samples from Tisza and Middle Danube section.Our study indicated the need for a careful planning of sterlet stocking programmes and inclusion of demographic data or catch time-series.

Key words: Acipenser ruthenus; mtDNA; population differentiation; gene flow

 

Corresponding author: Gorčin cvijanović, Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1a, 11000 Belgrade, Serbia, mitrandir@imsi.rs, tel. +381112078477, fax. +381113055289

 

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 1063-1078

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

              DOI: 10.2298/GENSR1503063C

             Original scientific paper

 

IDENTIFICATION OF MICROSATELLITE MARKERS LINKED TO THERMOTOLERANCE IN SILKWORM BY BULK SEGREGANT ANALYSIS

AND IN SILICO MAPPING

 

n. chandrakanth, s.m. moorthy^*, k.m. ponnuvel¹ and v. sivaprasad²

 

¹Silkworm Crop Improvement, Central Sericultural Research and Training Institute, Mysore (Karnataka), India

²Genomics Division, Seribiotech Research Laboratory, Bangalore (Karnataka), India

 

Abstract

Chandrakanth N., S.M. Moorthy, K.M. Ponnuvel and V. Sivaprasad (2015): Identification of microsatellite markers linked to thermotolerance in silkworm by bulk segregant analysis and in silico mapping - Genetika, Vol 47, No. 3, 1063-1078.

Silkworm, being a poikilothermic insect, its growth and development is affected by environmental factors especially, temperature. In tropical countries like India, it has considerable effect on silk production due to the prevailing of hot climatic conditions. Previous attempts to evolve silkworm breeds and hybrids tolerant to high temperature by traditional breeding methods have not yielded the desired results. Hence application of new strategies like marker assisted selection (MAS) could be the most effective strategy for developing a thermo-tolerant bivoltine silkworm for sustainable silk production in India. As a prelude, in this study it is aimed to identify simple sequence repeat (SSR) markers closely linked with thermotolerance in silkworm. To do so, 20 silkworm breeds were evaluated at high temperature (36°C) and based on pupation percentage, two multivoltines (Nistari and Cambodge) and two bivoltines (SK4C and BHR3) were identified as thermo-tolerant and one bivoltine (CSR2) was identified as the susceptible breed. These breeds were screened with 85 SSR markers drawn from different linkage groups and out of those, only 11 markers (12.9%) showed distinct polymorphism between thermo-tolerant and susceptible breeds. Further, bulked segregant analysis (BSA) was performed using 11 polymorphic SSR primers, by comparing the SSR profiles of the tolerant (Nistari) and susceptible (CSR2) parents, their F₁ and F₂ bulks. Nevertheless, only 5 markers generated clear differences in the amplified DNAs between the bulks corresponding to that of the parents suggesting that the DNA regions amplified by these SSR markers are closely linked to thermotolerance in B. mori. The results obtained through bulk segregant analysis was further confirmed by genotyping 5 linked SSR markers using 140 individual F₂ progenies. Of these 5 markers, highest Spearman's rho correlation coefficient was shown by S0816 indicating a high degree of closeness between the genotypic and phenotype variations in F₂ population. Furthermore, we have also attempted to locate the genes near to S0816 by in silico approach and upshot revealed 3 genes nearer to its sequence on the B. mori genome. The BGIBMGA005249 gene was found to be located nearest to S0816 at a distance of 14.8 Kb. But, further studies are required in this regard to derive a relationship between the thermotolerance and the functional role of identified genes nearer to the closest marker, so that the identified markers can be used to develop a thermo-tolerant silkworm breed through MAS.

Key words: Bulk segregant analysis, In silico mapping, Marker assisted selection, Silkworm, Thermotolerancež

Corresponding author: S.M. Moorthy,Silkworm Breeding and Molecular biology Laboratory, Silkworm Crop Improvement Division,Central Sericultural Research and Training Institute, Srirampura, Manandavadi Road, Mysore 570008, Karnataka, India, Phone: +91-9481821244,Fax: +91-821-2362845,e-mail: moorthysm68@gmail.com

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Published in „GENETIKA“ Vol. 47, No. 3(2015), pp. 1079-1090

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630

                          DOI: 10.2298/GENSR1503079L

Original scientific paper

 

 

VARIABILITY OF SEEDLINGS OF DOUGLAS-FIR PROVENANCES INTRODUCED FROM CANADA

Vera lavadinović ¹, Vukan lavadinović ², Ilija djordjević¹

 

 1- Institute of Forestry, Belgrade, Serbia

2- Faculty of Forestry, Belgrade

 

Department of Evolutionary Biology, Institute for Biological Research ”Siniša Stanković”, University of Belgrade, Belgrade, Serbia

 

 

Abstract

                       

lavadinović V., V. lavadinović, I. djordjević (2015): Variability of seedlings of douglas-fir provenances introduced from Canada.- Genetika, Vol 47, No. 3, 1079-1090.

Introduction of Canadian provenances of Douglas-fir (Pseudotsuga menziesii Mir / Franco) in Serbia started with the first phase of testing their genetic potential by studying the effects of geographic characteristics of the locations from which the provenances originated (latitude, longitude and altitude) on the variability of the measured seedling properties. In the laboratory of the Institute for Forestry in Belgrade, germinability of Douglas-fir seeds was tested on the germination table ("Copenhagen table" or "Jakobson table") by the standards of ISTA. The analysis of variance and the regression and correlation analysis were applied in the study of the effects of geographic parameters of Canadian provenance locations on the variability of seedlings. The results show that there is a statistically significant effect of the provenance latitude on the length of seedlings. The effect of altitude is slightly smaller, while the longitude of the provenance location has the smallest effect on the studied property. The study of the variability of Douglas-fir provenances in their juvenile development, as seedlings, is essential for reliable planning and implementation of further tests within pilot projects on allochthonous sites in Serbia.

Key words: introduction, Douglas-fir, provenances, variability, length of seedlings

 

Corresponding author: Vera Lavadinović, Institute of Forestry, Kneza Višeslava 3, Belgrade, 11030, Serbia. Phone: + 381-11-355-33-55. Email: veralava@eunet.rs

 

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Published in „GENETIKA“ Vol. 47 No. 3(2015), pp. 1091-1098

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

                           DOI: 10.2298/GENSR1503091D

                                                           Original scientific paper

 

 

 

CORRESPONDING ERDOSTEINE CHANGES AUTOPHAGY GENES EXPRESSION IN HIPPOCAMPUS ON RHINITIS MEDICAMENTOSA MODEL

Recep dokuyucu^1,2,*, Bulent gogebakan², Cengiz cevik³

 

¹ Department of Medical Physiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey

² Department of Molecular Biochemistry and Genetics, School of Medicine, Mustafa Kemal University, Hatay, Turkey

³ Department of Medical Biology, School of Medicine, Mustafa Kemal University, Hatay, Turkey

⁴ Department of  Otolaryngology, School of Medicine, Mustafa Kemal University, Hatay, Turkey

 

 

Abstract

dokuyucu R., B. Gogebakan, C.Cevik (2015): Corresponding erdosteine changes autophagy genes expression in hippocampus on rhinitis medicamentosa model.- Genetika, Vol 47, No. 3, 1091-1098.

In our study, rats were subjected to Oxymetazoline hydrochloride treatment and Rhinitis medicamentosa (RM) was formed and then autophagy gene expression levels were determined after the application of an antioxidant agent erdosteine (ED). The rats were divided into three groups; Group 1 was the control group. Group 2 (RM) and group 3 (RM+ED) rats received two spray puffs of 0.05% oxymetazoline into the nasal cavities three times daily for eight weeks. After determination of RM in the rats, the RM group were killed. The ED+RM group received 10 mg/kg of an ED suspension. At the end of seven days, these rats were also killed. All groups’ hippocampus tissues were obtained for the measurement of autophagy gene expressions. In rhinitis medicamentosa group Atg5, Atg7 and Atg10 gene expressions in the left hippocampus were reduced as compared to control group (p=0.01, p>0.05, p=0.01, respectively). Also, erdosteine treatments were restored mRNA expression of autophagy genes. In right hippocampus of rhinitis medicamentosa group, Atg5 and Atg10 gene expressions was found to be down-regulated as compared to control group (p>0.05, p<0.05, respectively). Both BECN1 and ULK genes expression were found to be reduced in left hippocampus of rhinitis medicamentosa group.  Erdosteine applications was restored the expression of these genes (p=0.03, p=0.03, respectively). Additionally, in right hippocampus, Erdosteine application was restored the expression of ULK gene (p=0.01).

This is the first report that evaluated the expression autophagy genes in RM rat models and the changes observed after erdosteine applications.

Key words: Rhinitis medicamentosa; Autophagy genes; Hippocampus; Erdosteine; Oxymetazoline

 

Corresponding author: Recep Dokuyucu, Assist. Prof. Dr., Department of Medical Physiology, Mustafa Kemal University, 31100, Hatay, Turkey,E-mail: drecepfatih@gmail.com, Phone: +90 555 267 0267,Fax: +90 326 224556

 

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 1099-1110

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

                       DOI: 10.2298/GENSR1503099M 

                Original scientific paper

 

 

INFLUENCE OF TOMATO GENOTYPE TO PHENOLIC COMPOUNDS CONTENT AND ANTIOXIDANT ACTIVITY AS REACTION TO EARLY BLIGHT

 

Slađana medić-pap¹, Dejan prvulović², Ana takač², Slobodan vlajić^2*,

Dario danojević¹, Adam takač¹, Stevan maširević²

 

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Serbia

 

 

Abstract

 

Medić-Pap S., D. prvulović, A. Takač, S. Vlajić, D. danojević, A.takač, S.maširevic (2015): Influence of tomato genotype to phenolic compounds content and antioxidant activity as reaction to early blight- Genetika, Vol 47, No. 3, 1099-1110.

Early blight is one of the most common and destructive tomato disease and it is caused by the fungus Alternaria solani. The aim of this paper was to screen the reaction of ten tomato genotypes (collection of the Institute of Field and Vegetable Crops) against natural infection of early blight. Tested genotypes showed significant differences in the disease occurrence on leaves but not on fruits. However, at the biochemical level, total phenolics (TP), tannins (TT), flavonoids (TF) and antioxidant activity in tomato fruits was significantly affected by genotype, disease occurrence and interaction of these two factors. According to obtained results, content of these secondary metabolites could be used as a one of the parameters in the evaluation of tomato resistance to EB.

Key words: tomato genotypes, early blight, polyphenol compounds, antioxidant activity

 

Corresponding author: Slađana Medić-Pap, Institute of field and vegetable crops, Maksima Gorkog 30, Novi Sad, Serbia phone +381 21 4898 352 e-mail: sladjana.medicpap@nsseme.com, ** PhD grant student of Ministry of education science and technological development

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 1111-1122

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

     DOI: 10.2298/GENSR1503111H

                      Original scientific paper

 

 

 

GENETIC STRUCTURE OF CULTIVATED FLAX (Linum usitatissimum L.) BASED ON RETROTRANSPOSON-BASED MARKERS

 

Hadi habibollahi¹, Zahra noormohammadi¹, Masoud sheidai², Farah farahani³

 

 

Abstract

 

Habibollahi H., Z. Noormohammadi, M. Sheidai, F. Farahani (2015): Genetic structure of cultivated flax (Linum usitatissimum l.) based on retrotransposon-based markers- Genetika, Vol 47, No. 3, 1111-1122.

Flax (Linum usitatissimum  L.) is one of the most important fiber and oil crop plants cultivated since ancient time. The flax seeds contain high amount of omega- 3-fatty acids and biologically active lignans. In spite of economic importance of cultivated flax, no information is available on its genetic variability and population structure in Iran. Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this crop plant. We used 30 randomly selected plants in three geographical populations for present investigation. AMOVA test produced significant genetic difference (PhiPT = 0.40, P = 0.010) among the studied populations and also revealed that, 40% of total genetic variability was due to within population diversity while, 60% was due to among population genetic differentiation. Gst (0.78, P = 0.001), Hedrick, standardised fixation index (G'st = 0.83, P = 0.001), revealed that the studied populations are genetically differentiated. STRUCTURE plot based on admixture model revealed that the studied populations differed extensively in their genetic content, but some degree of shared alleles occurred between them. Some adaptive IRAP loci were identified by LFMM analysis. These loci were private alleles restricted to geographical populations. Data obtained may be used in breeding and hybridization program of flax in the country.

                           Key words: flax, genetic differentiation, IRAP

 

Corresponding author: Hadi habibollah, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, Email: marjannm@yahoo.com , z-nouri@srbiau.ac.ir, Phone No: +982144865939, Fax No: +982144865939

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 1123-1130

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

DOI: 10.2298/GENSR1503123M

                                                                                                Original scientific paper

 

 

DNA MICROSATELLITE ANALYSIS FOR  TOMATO

GENETIC DIFFERENTIATION

 

Elizabeta miskoska - milevska¹, Zoran T. popovski ¹,

Blagica dimitrievska¹, Katerina bandzo²

 

University Ss.”Cyril and Methodius“ in Skopje

¹Faculty of Agricultural Sciences and Food - Skopje

            ² Institute of Agriculture - Skopje

 

 

Abstract

Miskoska – Milevska E., Z. T. popovski, B. dimitrievska, K. bandzo (2015): DNA microsatellite analysis for  tomato genetic differentiation - Genetika, Vol 47, No. 3, 1123-1130

Commonly used method for determination of the genetic diversity among the populations is the test for genetic differentiation. DNA microsatellite markers are usually used to investigate the genetic structure of natural populations. The aim of this study was to evaluate the applicability of eight DNA microsatellite loci (LECH13, LE21085, LEMDDNa, LEEF1Aa, LELEUZIP, LE20592, TMS9 and LE2A11) in genetic differentiation of six morphologically different tomato varieties (var. grandifolium from subsp. cultum; var. cerasiforme - red and yellow, var. pruniforme and var. pyriforme from subsp. subspontaneum; and var. racemigerum from subsp. spontaneum). The fragment analyses was performed using Applied Biosystems DNA analyzer (ABI 3130) and GeneMapper® Software program. The data were analysed using the specific program Power Marker Software. The average number of detected alleles was 3,625. Also, the average PIC value for all 8 DNA microsatellites loci was 0,3571. The genetic differentiation test in the researched tomato subspecies showed minor differentiation for locus LELEUZIP (- 0,0009), modest differentiation for locus LECH13 (0,0896), locus LEMDDNa (0,0896) and locus LE21085 (0,0551) and major differentiation for locus LE2A11 (0,7633), locus LEEF1Aa (0,6167), locus TMS9 (0.4967) and locus LE20592 (0,4263). On the other hand, in the estimated tomato varieties, locus LE21085 (0,0297), locus LECH13 (0,0256) and locus LELEUZIP (0,0005) showed minor differentiation, locus LEMDDNa (0,1333) showed modest differentiation, while locus TMS9 (0,5929), locus LEEF1Aa (0,5006), locus LE2A11 (0,4013) and locus LE20592 (0,2606) showed major differentiation. The eight DNA microsatellite loci can be applicable solution for tomato genetic differentiation. The overall results suggest that these microsatellite loci could be used in further population genetic studies of tomatoes.

Key words: DNA microsatellites, genetic differentiation, Lycopersicon esculentum, subspecies, varieties.

 

Corresponding author: Elizabeta Miskoska – Milevska, University Ss.”Cyril and Methodius”, Faculty of Agricultural Sciences and Food, Blvd. Edvard Kardelj bb. 1000 Skopje, R. Of Macedonia, Phone: +38923255161, Fax: +38923134310, e-mail: miskoska@yahoo.com

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 1131-1148

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

DOI: 10.2298/GENSR1503131A

                                                                                            Original scientific paper

 

 

 

USING CYTOGENETIC ANALYSIS RAPD IN DETERMINATION OF GENETIC VARIATIONS AMONG FOUR SPECIES OF ORNAMENTAL FISHES OF FAMILY: Poecilidae (Order: Cyprinodontiform)

ABU-ALMAATY, A. H.¹; Mary WELSON ZEKRY² & Yaseen A. ESSA²

 

¹Zoology Department, Faculty of science, Port Said University- Egypt.

²Zoology Department- Faculty of Science - Suez University- Egypt.

 

Abstract

 

Abu-Almaaty,A. H.,.M. Welson Zekry and Y. A. Essa (2015): Using cytogenetic analysis and RAPD in determination of genetic variations among four species of ornamental fishes of family: Poecilidae (order: Cyprinodontiform).- Genetika, Vol 47, No. 3, 1131-1148.

The karyological and molecular analysis of four fresh water fish species of Family: Poecilidae and their genetic relationships have been studied. Xiphophorus maculates and Xiphophorus hellerii have the same diploid chromosome number 2n=48, but they were different in their karyotypes.  Poecilia sphenops and Poecilia reticulata have the same diploid chromosome number 2n=46 and the same fundamental number FN=46, also the same karyotype one group of acrocentric chromosomes. Nine RAPD primers, showed monomorphic bands, were used for the construction of the dendrogram and a similarity matrix. A total of 65 bands were obtained; 39 of them were monomorphic bands. Similarity values among the studied samples ranged from 21% to 38%. High similarity value was obtained between Xiphophorus maculates and Xiphophorus hellerii. (38%) and the low similarity values were obtained between Xiphophorus hellerii and Poecilia reticulata (21%). The cluster analysis clearly differentiated Xiphophorus maculates and Xiphophorus hellerii from Poecilia  sphenops and Poecilia reticulata. RAPD analysis confirmed that the four species under study are genetically different from each other. These cytogenetic data obtained can be applied for further studies in cytotaxonomy and evolutionary relationships of fishes.

Key words: Cytogenetics, karyotype, RAPD,PCR,DNA,Fishes,Poecilidae Xiphophorus, Poecilia.                                                                                                                                                                                        

 

Corresponding author: Ali Hussein Abu -Almaaty, PhD., Biotechnology Program, Department of Zoology, Faculty of Science, Port Said University, Port Said, 42521, Egypt,Tel:  00201093041699, E-mail: ali_zoology_2010@yahoo.com

 

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Published in „GENETIKA“ Vol. 47, No.3(2015), pp. 1149-1164

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633

  DOI: 10.2298/GENSR1503149M

                                                                                                   Original scientific paper

 

 

 

 

 

AGROBACTERIUM-MEDIATED TRANSFORMATION OF ORNAMENTAL SPECIES:

 A REVIEW

 

Snežana milošević*, Aleksandar cingel, Angelina subotić

 

Institute for Biological Research „Siniša Stanković“, University of Belgrade, Belgrade

 

Abstract

 

Milošević S., A. Cingel, A.Subotić (2015): Agrobacterium-mediated transformation of ornamental species: a review- Genetika, Vol 47, No. 3, 1149-1164.

Integration of desirable traits into commercial ornamentals using genetic engineering techniques is a powerful tool in contemporary biotechnology. However, these techniques have had a limited impact in the domain of ornamental horticulture, particularly floriculture. Modifications of the color, architecture or fragrance of the flowers as well as an improvement of the plant tolerance/resistance against abiotic and biotic stresses using plant transformation techniques, is still in its infancy. This review focuses on the application of Agrobacterium-mediated transformation, a major plant genetic engineering approach to ornamental plant breeding and the impact it has had to date.

Key words: Agrobacterium rhizogenes; A. tumefaciens; genetic transformation; ornamental species

 

Corresponding author:  Snežana Milošević,Institute for Biological Research, University of Belgrade,  Bul. despota Stefana 142, 11060 Belgrade, Serbia, tel: + 381 11 2078393, fax: + 381 11 2761433, e-mail: snezana@ibiss.bg.ac.rs

 

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Published in „GENETIKA“ Vol. 47, No.2(2015), pp. 765-776

© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

   DOI: 10.2298/GENSR1502765C

                                                                                             Original scientific paper

 

 

 

ANTHROPOGENETIC VARIABILITY IN THE GROUPS OF HOMO-

AND HETEROSEXUALLY ORIENTED INDIVIDUALS

 

Suzana CVJETICANIN

 

Institute of Human Genetics, School of Medicine, University of Belgrade

 

Abstract

 

 

Cvjeticanin S. (2015): Anthropogenetic variability in the groups of homo- and heterosexually oriented individuals- Genetika, Vol 47, No. 2, 765-776.

This population-genetic study compares morphophysiological and genetic variability in a group of homosexually oriented individuals from Serbia (N=96) with control group of heterosexual individuals (N=96) using a test of determination of homozygously recessive characteristics in humans (HRC-test).

Results of our study revealed a statistically significant difference in the mean values of genetic homozygosity (control group 5.0+0.2 ; homosexuals 3.4 +0.1 HRCs, out of 20 observed characteristics) the differences in the distribution type, as well as in the variances of presence of specific combinations of such traits. These results suggest a complex polygenic difference between two observed systems.

There is no difference in the degree of genetic homozygosity between the genders in each tested group of individuals. However, both homosexually oriented females and males have significantly lower mean values of HRCs compared to female and male heterosexuals.

Key words: anthropogenetic variability, genetic homozygosity, homosexuals, homorecessive  characteristics

 

Corresponding author: Suzana Cvjeticanin, Institute of Human Genetics, School of Medicine, University of Belgrade,Visegradska 26, 11000 Belgrade, Serbia,Tel/Fax. +381 11 3607040,Tel   +381 60 3534821,E-mail: cujasimsi@gmail.com

 

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