GENETIKA, Vol. 48, No.2 (2016)
Amir Ali khalatbari, Hawa Z.E. jaafar, Amir Mahdi khalatbari, Maziah mahmood and Radziah othman
ANALYSIS OF SECONDARY METABOLISM AND TOTAL CHLOROPHYLL CONTENT PROVIDES NEW INSIGHTS INTO THE ROLE OF Α-TOCOPHEROL FOR WILD TYPE AND VTE4 MUTANT Arabidopsis thaliana UNDER DIFFERENT ABIOTIC STRESSES [Abstract] [Full text]
Borivoj pejić, Ksenija mačkić, Dragiša milošev, Erkut peksen, Srdjan šeremešić, Goran jaćimović, Vladimir ćirić
RESPONSE OF COWPEA GENOTYPES TO WATER STRESS IN TEMPERATE CLIMATIC CONDITIONS [Abstract] [Full text]
Amir GHOLIZADEH and Hamid DEHGHANI
GRAPHIC ANALYSIS OF TRAIT RELATIONS OF IRANIAN BREAD WHEAT GERMPLASM UNDER NON-SALINE AND SALINE CONDITIONS USING THE BIPLOT METHOD [Abstract] [Full text]
Danijela ristic, Ivan vucurovic, Slobodan kuzmanovic, Drago milosevic, Katarina gasic, Nenad dolovac, Mira starovic
CORRELATION AND PATH COEFFICIENT ANALYSIS FOR PROTEIN YIELD IN CONFECTIONARY SUNFLOWER (Helianthus annuus L.) [Abstract] [Full text]
Masoud sheidai, Mona naji, Zahra noormohammadi, Maryam nouroozi, Somayeh ghasemzadeh-baraki
CONTEMPORARY INTER-SPECIFIC HYBRIDIZATION BETWEEN Cirsium aduncum AND C. Haussknechtii (Asteraceae): EVIDENCE FROM MOLECULAR AND MORPHOLOGICAL DATA [Abstract] [Full text]
Simone perna, Chiara bologna, Pietro cavagna, Luisa BERNARDINELLI, Davide GUIDO, Gabriella PERONI, Mariangela RONDANELLI
THE BEGINNINGS OF ALZHEIMER’S DISEASE: A REVIEW ON INFLAMMATORY, MITOCHONDRIAL, GENETIC AND EPIGENETIC PATHWAYS [Abstract] [Full text]
Peter dolničar, Drago milošević, Zoran jovović, Vladimir meglič, Marko maras and Ana velimirović
Reliability of morphological AND MOLECULAR CHARACTERIZATION of lightsprouts FOR differentiation of potato accessions [Abstract] [Full text]
Rodrigo pérez-sánchez, María Remedios morales-corts, María Ángeles gómez-sánchez
AGro-morphological diversity of traditional fig cultivars grown in Central-Western Spain [Abstract] [Full text]
Yonca SURGUN, Bekir ÇÖL, Betül BÜRÜN
DIFFERENTIAL EXPRESSION ANALYSIS OF BORON TRANSPORTERS AND SOME STRESS-RELATED GENES IN RESPONSE TO 24-EPIBRASSINOLIDE AND BORON BY SEMI-QUANTITATIVE RT-PCR IN Arabidopsis thaliana (L.) Heynh [Abstract] [Full text]
Raheleh tabaripoor, Masoud sheidai Seyyed Mehdi talebi Zahra noormohammadi
POPULATION GENETIC DIVERSITY AND STRUCTURE IN Ziziphora tenuior L.: IDENTIFICATION OF POTENTIAL GENE POOLS [Abstract] [Full text]
Desimir knežević, Violeta maklenović, Ljubiša kolarić, Danica mićanović Andrej šekularac, Jasmina knežević
VARIATION AND INHERITANCE OF NITROGEN CONTENT IN SEED OF WHEAT GENOTYPES (Triticum aestivum L.) [Abstract] [Full text]
Samira ASGHARZADE, Morteza HASHEMZADE CHALESHTORI, Mohammad Amin TABATABAIFAR, Somayeh REISI Mohammad Hossein MODARESSI
MUTATION IN SECOND EXON OF MYO15A GENE CAUSE OF NONSYNDROMIC HEARING LOSS AND ITS ASSOCIATION IN THE ARAB POPULATION IN IRAN[Abstract] [Full text]
Noushafarin KARAMI, Ali AALAMI, Habibollah Samizadeh LAHIJI, Babak RABIEI and Mehrzad ALAHGHOLIPOUR
ANALYSIS AND COMPARISON OF FRAGRANT GENE SEQUENCE IN SOME RICE CULTIVARS [Abstract] [Full text]
Branko tomic, Maja gvozdenov, Iva pruner, Mirjana kovac, Nebojsa antonijevic, Dragica radojkovic, Valentina djordjevic
THE FREQUENCIES OF FV LEIDEN AND FII G20210A MUTATIONS IN PATIENTS WITH DIFFERENT CLINICAL MANIFESTATIONS OF VENOUS THROMBOEMBOLISM: EXPERIENCE FROM LARGE SERBIAN COHORT [Abstract] [Full text]
Stefan dačić, Ninoslav djelić, Milena radaković, Nada lakić, Aleksandar veselinović, Marko ristanić, Marko andjelković
EFFECTS OF PHOTOPOLYMERISATION ON GENOTOXICITY OF COMPOSITE ADHESIVES IN THE COMET ASSAY [Abstract] [Full text]
Tomas kiss, Tomas necas, Jana necasova
COMPARISON OF REAL-TIME PCR PROTOCOLS IN DETECTION AND QUANTIFICATION OF FRUIT TREE 16SRX GROUP PHYTOPLASMAS [Abstract] [Full text]
Baoyan jia, Xinhua zhao, Yang qin, Muhammad irfan, Tae-heon kim, Bolun wang, Shu wang, Jae Keun sohn
IDENTIFICATION OF QUANTITATIVE TRAIT LOCI FOR LEAF TRAITS IN RICE [Abstract] [Full text]
Halil Barış ÖZEL, Sezgin AYAN, Vasilije ISAJEV
INTER POPULATION VARIABILITY OF FROST-RESISTANCE IN PROVENANCES OF SCOT PINES (Pinusylvestris L. R. hamata Steven) IN TURKEY [Abstract] [Full text]
Bachubhai Arjanbhai monpara
SESAME GERMPLASM EVALUATION FOR REPRODUCTIVE PERIOD AND HARVEST INDEX [Abstract] [Full text]
Naser sabaghnia, Mehdi mohebodini, Mohsen janmohammadi
BIPLOT ANALYSIS OF TRAIT RELATIONS OF SPINACH (Spinacia oleracea L.) LANDRACES [Abstract] [Full text]
Milan stevanovic, Zoran camdzija, Jovan pavlov, Ksenija markovic, Jelena vancetovic, Snezana mladenovic drinic, Milomir filipovic
THE APPLICATION OF PROTEIN MARKERS IN CONVERSION OF MAIZE INBRED LINES TO THE CYTOPLASMIC MALE STERILITY BASIS [Abstract] [Full text]
Aleksandra POPOVAC, Ivica STANČIĆ, Nebojša DESPOTOVIĆ, Nađa NIKOLIĆ, Elka STEFANOVA, Jelena MILAŠIN
Difference in apolipoprotein E genotype distribution between dentate and edentulous elderly patients with Alzheimer disease [Abstract] [Full text]
Jelica, R. PANTELIĆ, Tatjana, J. VARLJEN, Nela, S MAKSIMOVIĆ, Biljana, B. JEKIĆ, Ana, J. OROS, Tatjana, V. NIKOLIĆ, Ivan, B. STEFANOVIĆ, Ivana, V. NOVAKOVIĆ and Tatjana, M. DAMNJANOVIĆ
ANALYSIS OF T-786C And 4a/b ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE POLYMORPHISMS IN RETINOPATHY OF PREMATURITY [Abstract] [Full text]
Tofig ALLAHVERDIYEV
YIELD AND YIELD TRAITS OF DURUM WHEAT (Triticum durum desf.) AND BREAD WHEAT (Triticum aestivum L.) GENOTYPES UNDER DROUGHT STRESS [Abstract] [Full text]
Marko MARAS, Afrodita Ibusoska, Suzana Kratovalieva, Rukie Agić, Jelka Šuštar-Vozlič, Vladimir Meglič
Genetic diversity of common bean accessions from Former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers [Abstract] [Full text]
Slavko BRANKOVIĆ and Suzana CVJETIĆANIN
ANTHROPOGENETIC VARIABILITY IN GROUPS OF CHILDREN FROM REGULAR AND SPECIAL SCHOOLS FROM DIFFERENT LOCALITIES IN SERBIA [Abstract] [Full text]
Nenad bukvic, Francesca boaretto, Giuseppe loverro, Francesco c. susca, Rosaura lovaglio, Margherita patruno, Dragoslav bukvic, Srdjan starcevic, Giovanni vazza, Maria Luisa mostaciuollo, Nicoletta resta
Familly with two different cases of post- and pre-natal l1 syndrome; when hydrocephaly become “multidisciplinary headache [Abstract] [Full text]
Hafiz Ghazanfar abbas, Abid mahmood and Qurban ali
ZERO TILLAGE: A POTENTIAL TECHNOLOGY TO IMPROVE COTTON YIELD [Abstract] [Full text] [Supplement] [Supplement]
Jelena m. Aleksić
Family-specific vs. universal PCR primers for the study of mitochondrial DNA in plants [Abstract] [Full text] [Supplement] [Supplement]
Corrigendum
Janković et al., Genetika 48(1):409-421 [Abstract] [Full text]
Corrigendum
Ashrafi F., A.Hashemi, K. Mardani, and R. Darvishzadeh (2014): Study on genetic variability in MHC-DRB1 second exon in makuie sheep breedpopulation. Genetika, Vol 46, No. 1, 269-275. [Abstract] [Full text]
Corrigendum
Ignjatovic-Micic D., D. Ristic, V. Babic, V. Andjelkovic and J. Vancetovic (2015): A simple SSR analysis for genetic diversity estimation of maize landraces.-Genetika, Vol 47, No. 1,53-62. [Abstract] [Full text]
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 445-462
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.630
Orginal scientific paper
ANALYSIS OF SECONDARY METABOLISM AND TOTAL CHLOROPHYLL CONTENT PROVIDES NEW INSIGHTS INTO THE ROLE OF Α-TOCOPHEROL FOR WILD TYPE AND VTE4 MUTANT Arabidopsis thaliana UNDER DIFFERENT ABIOTIC STRESSES
Amir Ali khalatbari¹*, Hawa Z.E. jaafar¹*, Amir Mahdi khalatbari¹,
Maziah mahmood2 and Radziah othman3
1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
2Department of Biochemistry, Faculty of Biotechnology and Biomolecular sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
3Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
Abstract
khalatbariA.A., Hawa Z.E. jaafar, A.M. khalatbari, M. mahmood and R. othman (2016): Analysis of secondary metabolism and total chlorophyll content provides new insights into the role of α-tocopherol for wild type and vte4 mutant Arabidopsis thaliana under different abiotic stresses. - Genetika, vol 48, no. 2,445 -462.
Plants experience different abiotic stresses under natural conditions including salinity, water deficit, low temperature and high light. Once plants are exposed to these stresses they might have a variety of responses physiologically and biochemically. In this study, we test this hypothesis in wild type Col-0 and vte4 mutant of Arabidopsis thaliana by measuring major secondary metabolites alongside with total chlorophyll content under different abiotic stresses namely salt stress, water stress and prolonged water deficiency. These stresses were imposed to the plants in separate experiments in which each treatment was replicated three times in a complete randomized design with factorial arrangement. It was concluded that under all abiotic stresses wild type Col-0 Arabidopsis plants showed stronger performance in terms of all major metabolites compared to vte4 mutant. α-tocopherol deficiency in vte4 mutant plants led to lower accumulation of proline, total protein and total amino acids as well as starch and total sugars in comparison with wild type A.thaliana. Furthermore, all five secondary metabolites obtained the highest value under 100mM NaCl concentration (Salt stress), under 50% of field capacity (water stress) and under 8 days of water withholding (prolonged water deficiency). Wild type Col-0 resulted in higher level of total chlorophyll content under all abiotic stresses compared to mutant plants. Therefore, our results suggested that the loss of α-tocopherol in vte4 mutant A.thaliana under different abiotic stresses affected the efficiency and the stability of central metabolism and photosynthetic apparatus.
Keywords: Arabidopsis thaliana, abiotic stresses, mutantm secondary metabolites, Wild type
Corresponding author: Amir Ali khalatbari, Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, 43400, Phone number: 0060389471807-00989369439618,email .amkh_united2002@yahoo.com- drhawazej.postgrads@gmail.com.
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 463 -472
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
Original scientific paper
RESPONSE OF COWPEA GENOTYPES TO WATER STRESS IN TEMPERATE CLIMATIC CONDITIONS
Borivoj pejić1, Ksenija mačkić1, Dragiša milošev1, Erkut peksen2, Srdjan šeremešić1, Goran jaćimović1, Vladimir ćirić1
1University of Novi Sad, Faculty of Agriculture, Serbia
2Ondokuz Mayis University, Faculty of Agriculture, Department of Field Crops, Samsun, Turkey
Abstract
Pejić B., K. Mačkić, D. Milošev, D., E. Peksen, S. Šeremešić, G. Jaćimović and V. Ćirić (2016): Response of cowpea genotypes to water stress in temperate climatic conditions - Genetika, Vol 48, No.2,463 -472.
Mitigation of global climate change impact on the agricultural production is the major priorities in future research. Cowpea as a drought tolerant plant is interesting for growing in semi-arid climate of the Vojvodina region. The effect of water stress on yield of cultivated plants can be obtained by calculating the yield response factor (Ky) which represents the ratio between the relative evapotranspiration deficit (1-ETa/ETm) and the relative decline in yield (1-Ya/Ym). The values of Ky ranged from 0.91 to 1.17 for genotype G1 and G2 respectively. Genotype G1, with a value lower than 1 of Ky, shows a good tolerance to water deficit, on the contrary, genotype G2, with a greater Ky than 1, expresses some sensitivity to water stress. Obtained results will be used in breeding programs to develop cowpea cultivars tolerant to stressful conditions, primarily to water stress, as well as more productive in water use.
Keywords: cowpea (Vigna unguiculata (L.) Walp.), water stress, yield, irrigation
Corresponding author: Borivoj Pejić, University of Novi Sad, Faculty of Agriculture, Sq. Dositeja Obradovica 8, 21000 Novi Sad, Serbia, Phone: +381 21 4853 229, Fax.: +381 21 459 761, e-mail: pejic@polj.uns.ac.rs
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 473- 486
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630.11
Original scientific paper
GRAPHIC ANALYSIS OF TRAIT RELATIONS OF IRANIAN BREAD WHEAT GERMPLASM UNDER NON-SALINE AND SALINE CONDITIONS USING
THE BIPLOT METHOD
Amir GHOLIZADEH and Hamid DEHGHANI,*
Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Abstract
Gholizadeh A. and H. Dehghani (2016): Graphic analysis of trait relations of Iranian bread wheat germplasm under non-saline and saline conditions using the biplot method.- Genetika, Vol 48, No. 2, 473 -486.
Salinity is one of the most important factors that
limit crop production in some regions of the world. Knowledge
of the interrelationships between yield and its components will improve the
efficiency of breeding programs especially under saline conditions through
appropriate selection criteria. This study demonstrated that GT biplot was an
excellent tool for visual evaluation of superior genotypes, traits and grouping
of them with other statistical techniques. The study was conducted under both
saline and non-saline conditions in field based on randomized complete block
design with three replications. Electrical conductivity of irrigation water
were 2 and 10 dS.m-1 in non-saline and saline conditions,
respectively. The obtained data were analyzed using a genotype 
trait (GT) biplot method based on site regression model.
The biplot vector view indicate that there was a strong positive association
between PH and BY with seed yield in both non-saline and saline conditions. It
seems that PH and BY traits can be used as selection criterion for improving of
seed yield in wheat breeding programs, especially under stress conditions in the
field. Also among 41 studied genotypes, genotype 32 had good characteristics
regarding high seed yield and salt tolerance.
Keywords: Biplot, Salinity, Seed yield, Site regression.
Corresponding author: Hamid Dehghani, Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran, Email: dehghanr@modares.ac.ir. Phone: +98 912 5144820. Fax: +98 48292040
Published in „GENETIKA“ Vol. 48, No2 (2016), pp. 487-496
©
2016Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
Original scientific paper
MOLECULAR CHARACTERIZATION OF POTATO VIRUS Y INDUCING POTATO TUBER NECROTIC RINGSPOT DISEASE IN SERBIA
Danijela ristic1, Ivan vucurovic1, Slobodan kuzmanovic1, Drago milosevic2,
Katarina gasic1, Nenad dolovac1, Mira starovic1
1Institute for Plant Protection and Environment, Department of Plant Pathology, Belgrade, Serbia
2University of Kragujevac, Faculty of Agronomy, Čačak, Serbia
Abstract
ristic D., I. Vucurovic, S. Kuzmanovic, D. milosevic, K. gasic, N. dolovac, M.starovic (2016): Molecular characterization of potato virus y inducing potato tuber necrotic ringspot disease in Serbia.-Genetika, vol 48 , no.2, 487-496.
The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate’s complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
Keywords: Potato, PVY, Genome, PTNRD, diversity
Corresponding author: Danijela Ristic, Institute for Plant Protection and Environment, Department of Plant Pathology, Teodora Drajzera 9, Belgrade, Serbia, Tel: +381 11 2660049, Fax: +381 11 2669860, E-mail: risticdaca@yahoo.com
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp. 497-514
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
CONTEMPORARY INTER-SPECIFIC HYBRIDIZATION BETWEEN Cirsium aduncum AND C. Haussknechtii (Asteraceae): EVIDENCE FROM MOLECULAR AND MORPHOLOGICAL DATA
Masoud sheidai*1, Mona naji1, Zahra noormohammadi2, Maryam nouroozi3, Somayeh ghasemzadeh-baraki1
1Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
2 Department of Biology, School of Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
3Department of Horticulture, collage of Aburaihan, University of Tehran, Tehran
Abstract
sheidai M., Ma naji, Z. noormohammadi, M.nouroozi, S. ghasemzadeh-baraki (2016): Contemporary inter-specific hybridization between Cirsium aduncum and C. haussknechtii (Asteraceae): evidence from molecular and morphological data- Genetika, Vol 48, No. 2,497-514.
Cirsium aduncum Fisch. & C.A.Mey. Ex DC. and C. haussknechtii Boiss., (Asteraceae) are important medicinal plant species that grow in different geographical regions of Iran. We had no knowledge about population genetic structure, intra-specific and inter-specific gene flow and the presence of hybrid zone for this two species in Iran. Therefore, in order to provide data for conservation of these two medicinally important species, the population genetic analysis and morphometric studies were performed in 18 geographical populations of these species. ANOVA and MDS analyses revealed significant morphological difference among the studied populations in either species, while MDS plot showed morphological overlap in plants of these two species. AMOVA test revealed significant genetic difference among the studied populations. Mantel test showed positive significant correlation between genetic and geographical distances and the occurrence of isolation by distance. Population assignment test and STRUCTURE plot of genetic data revealed inter-specific introgression between these species.
Keywords: AMOVA, Circium, gene flow, population assignment, STRUCTURE analysis.
Corresponding author: Masoud sheidai, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran, email: msheidai@yahoo.com, Tel: +98 9122593378
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 515 -524
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
THE BEGINNINGS OF ALZHEIMER’S DISEASE: A REVIEW ON INFLAMMATORY, MITOCHONDRIAL, GENETIC AND EPIGENETIC PATHWAYS
Simone perna1, Chiara bologna1, Pietro cavagna2, Luisa BERNARDINELLI2, Davide GUIDO 123, Gabriella PERONI 1, Mariangela RONDANELLI1
1University of Pavia, Department of Public Health, Experimental and Forensic Medicine, Section of Human Nutrition, Endocrinology and Nutrition Unit, Azienda di Servizi alla Persona ‘‘Istituto Santa Margherita’’, Pavia, Italy;
2University of Pavia, Department of Brain and Behavioral Sciences, Medical and Genomic Statistics Unit, Pavia, Italy;
3University of Pavia, Department of Public Health, Experimental and Forensic Medicine, Biostatistics and Clinical Epidemiology Unit, Pavia, Italy
Abstract
perna S., C. bologna, P. cavagna, l. Bernardinelli, D. Guido, g. Peroni, m. Rondanelli (2016): The beginnings of Alzheimer’s disease: a review on inflammatory, mitochondrial, genetic and epigenetic pathways.- Genetika, Vol 48, No. 2, 515-524.
Alzheimer’s Disease (AD) is the most common cause of sporadic dementia, as it affects 60% of cognitive impaired patients; it commonly affects middle and late life, and it is considered an age-related disease. Early-onset familial AD is associated with mutations of the genes encoding amyloid precursor protein (APP), presenilin 1 (PS-1), or PS-2, resulting in the overproduction of amyloid beta-protein. Epidemiological and case-control studies have led to the identification of several risk factors for sporadic AD. The most concrete genetic risk factor for AD is the epsilon4 allele of apolipoprotein E gene (APOE). In addition, several genes such as CTNNA3, GAB2, PVRL2, TOMM40, and APOC1 are known to be the risk factors that contribute to AD pathogenesis. A direct role of interaction between genetic and environmental determinants has been proposed in an epigenetic dynamic for environmental factors operating during the preconceptual, fetal and infant phases of life. Also the the association between mtDNA inherited variants and multifactorial diseases and AD has been investigated by a number of studies that, however, didn’t reach a general consensus on the correlation between mtDNA haplogroups and AD
Keywords: genetics, epigenetics, Alzheimer, dementia, mitochondrial genome, genes
Corresponding author: Simone Perna; PhD, Department of Public Health, Experimental and Forensic Medicine, Section of Human Nutrition and Dietetics, Azienda di Servizi alla Persona di Pavia, University of Pavia, Via Emilia 12, Pavia, Italy. E-mail: simoneperna@hotmail.it tel: 00390382381706
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp 525 -532
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
Original scientific paper
Reliability of morphological AND MOLECULAR CHARACTERIZATION of lightsprouts FOR differentiation of potato accessions
Peter dolničar1, Drago milošević3, Zoran jovović2, Vladimir meglič1, Marko maras1 and Ana velimirović2
1Agricultural Institute of Slovenia, Slovenia
2University of Montenegro, Biotechnical Faculty Podgorica, Montenegro
3University of Kragujevac, Faculty of Agronomy in Čačak, Serbia
Abstract
dolničar P., D. milošević, Z. jovović, V. meglič, M. maras and A. velimirović (2016): Reliability of morphological and molecular characterization of lightsprouts for differentiation of potato accessions--Genetika, Vol 48, No. 2, 525-532.
The study of reliability of morphological characterization of lightsprouts for differentiation of potato varieties was performed at the Agricultural Institute of Slovenia in cooperation with Biotechnical Faculty Podgorica in order to introduce simple method for further characterization of potato accessions in Montenegrin gene bank. Seven selected, potentially different, potato accessions preserved in the Montenegrin gene bank were used for morphological characterization of lightsprouts. Using UPOV guidelines 11 lightsprout traits were estimated. Molecular assessment was carried out in parallel with morphological characterization by six microsatellite (SSR) markers. The latter successfully distinguished all accessions but two, while four different lightsprout phenotypes were identified in morphological characterization. Though molecular markers showed more strength in resolving relationships between genotypes, characterization of lightsprouts still demonstrated its usefulness due to cheap, simple and rapid procedure.
Keywords: morphological characterization of lightsprouts, molecular differentiation, potato accessions, SSR markers
Corresponding author: Peter Dolničar, Agricultural institute – Ljubljana, Hacquetova ulica 17, 1000 Ljubljana, Slovenia, e-mail: peter.dolnicar@kis.si
Published in „GENETIKA“ Vol. 48 No.2 (2016), pp. 533-546
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
AGro-morphological diversity of traditional fig cultivars grown in Central-Western Spain
Rodrigo pérez-sánchez*, María Remedios morales-corts,
María Ángeles gómez-sánchez
Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, Spain
Abstract
pérez-sánchez R., M. R. morales-corts, M. A. gómez-sánchez (2016): Agro-morphological diversity of traditional fig cultivars grown in central-western Spain- Genetika, Vol 48, No. 2, 533-546.
There are not many exhaustive works addressing the agromorphological characterization of traditional fig (Ficus carica L.) cultivars in Spain. In order to analyze the diversity of these fig genetic resources, twelve traditional fig cultivars from the Central-Western Region of Spain were surveyed and characterized agromorphologically. A total of forty descriptors, mainly defined by the International Plant Genetic Resources Institute and the International Union for the Protection of New Varieties of Plants, were used to describe the fruits, leaves and the tree itself over two consecutive years (2013-2014). Some of the cultivars showed distinctive and interesting agronomical characters from a commercial point of view, such as two crops per year (breba and fig), high yields, and fruit quality. This was the case of the fig cultivar widely distributed through the Duero river valley called ‘Cuarterón’. Its fruits were quite heavy and sweet (breba: 93.75 g and 25.91º Brix; fig: 42.41 g and 31.50º Brix), easy to peel, and juicy. Principal component analysis revealed that more than 67% of the agromorphological variability observed was explained by the first three components, some of the breba size parameters (fruit and neck length and fruit width) being the most important factors in differentiating the genotypes. A dendrogram clustered the cultivars into two major groups (unifera and bifera type) and revealed existing synonymies and homonymies. ‘Carballar Negra’ and ‘Moscatel’ were the only fig cultivars which did not have breba crops. This work is an important step in the conservation of genetic fig resources in Spain.
Keywords: Ficus carica, fig descriptors, conservation, endangered cultivars.
Corresponding author: Rodrigo Pérez-Sánchez, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Avda. Filiberto Villalobos, 119. 37007-Salamanca, Spain, Phone: 34-923-29-4690; Fax: 34-923-29-4774; E-mail: rodrigopere@usal.es
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp. 547-563
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
DIFFERENTIAL EXPRESSION ANALYSIS OF BORON TRANSPORTERS AND SOME STRESS-RELATED GENES IN RESPONSE TO 24-EPIBRASSINOLIDE AND BORON BY SEMI-QUANTITATIVE RT-PCR IN Arabidopsis thaliana (L.) Heynh
Yonca SURGUN1,2*, Bekir ÇÖL3, Betül BÜRÜN3
1Department of Biology, Graduate School of Natural and Applied Sciences, Mugla Sıtkı Kocman University, Mugla, Turkey
2Department of Molecular Biology and Genetics, Faculty of Science, Bartın University, Bartın, Turkey
3Department of Biology, Faculty of Science, Mugla Sıtkı Kocman University, Mugla, Turkey
Abstract
Surgun S Y., B. Çöl, B. Bürün (2016): Differential expression analysis of boron transporters and some stress-related genes in response to 24-epibrassinolide and boron by semi-quantitative RT-PCR in Arabidopsis thaliana (L.) Heynh.- Genetika, Vol 48, No. 2, 547-563.
Plant steroidal hormones, brassinosteroids (BRs), promote plant developmental processes and enhance tolerance to several abiotic stresses including high boron (B) stress. To examine the possible role of BR in high B-induced stress at the transcriptional level, we investigated the response of B transporter genes (BOR1-4), high B-induced genes (MATE, Hsp-like), BR-induced genes (Hsp70-4, Hsp90-1) and other stress-related genes (LTI/COR78, LEA4-5) upon exogenous treatments of 24-epibrassinolide (EBL) on Arabidopsis thaliana (L.) Heynh exposed to high concentrations of boric acid (BA) using semi-quantitative RT-PCR. BA treatments led to down regulation of BOR1 and BOR3 genes in leaf and root tissues and higher concentration of EBL further decreased expression of these genes in roots. The expression of high B-induced genes was observed to be upregulated by 1 µM EBL treatment under high B stress in both tissues of the seedlings. The upregulation of BR-induced genes were clearly evident in root tissues co-treated with 1 µM EBL and BA as compared to BA alone. Higher concentration of EBL was found to be more effective in increasing expression of LTI/COR78 gene in root and LEA4-5 gene in shoot tissues. To our knowledge, this is the first report how exogenous application of EBL modulates high B stress responses at molecular level in model plant Arabidopsis thaliana.
Key words: Arabidopsis thaliana, boron, 24-epibrassinolide, gene expression, semi-quantitative RT-PCR, stress.
Corresponding author: Yonca Surgun, Department of Molecular Biology and Genetics, Faculty of Science, Bartın University, 74000, Bartın, Turkey, Phone: +90 378 223 54 19, Fax: +90 378 223 52 23, e-mail: yoncasurgun@gmail.com
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 565 - 578
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
POPULATION GENETIC DIVERSITY AND STRUCTURE IN Ziziphora tenuior L.: IDENTIFICATION OF POTENTIAL GENE POOLS
Raheleh tabaripoor 1*, Masoud sheidai 1, Seyyed Mehdi talebi 2, Zahra noormohammadi3.
1 Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
2 Department of Boilogy, Faculty of Science, Arak University, Arak, Iran.
3 Department of Boilogy, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
tabaripoor R., M. sheidai, S. M. talebi, Z. noormohammadi (2016): Population genetic diversity and structure in Ziziphora tenuior L.: identification of potential gene pools.- Genetika, Vol 48, No. 2, 565-578.
Ziziphora tenuior L. is a medicinal plant species of the genus Ziziphora (Labiatae) that grows in different areas of Iran. In order to study the population genetic structure in Ziziphora tenuior,we collected 107 plant specimens from 20 geographical populations that are located in 17 provinces. ISSR molecular markers were used for genetic diversity analysis. The populations studied revealed intra- and inter-population genetic variability. AMOVA test showed significant genetic difference among the studied populations. STRUCTURE plot identified two main gene pools for Ziziphora tenuior in Iran. These populations showed isolation by distance and restrict gene flow occurred among them.
Keywords: Genetic diversity, gene flow, isolation by distance, Ziziphora tenuior.
Corresponding author: Raheleh tabaripoor, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran, Tel/Fax: +98 2129902111, Email address: raheleh.tp@gmail.com
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp.579- 586
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630.11
Original scientific paper
VARIATION AND INHERITANCE OF NITROGEN CONTENT IN SEED OF WHEAT GENOTYPES (Triticum aestivum L.)
Desimir knežević1, Violeta maklenović2, Ljubiša kolarić2,
Danica mićanović3 Andrej šekularac1, Jasmina knežević1
1University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lesak, Kosovo and Metohia, Serbia
2University of Belgrade, Faculty of Agriculture Belgrade, Zemun, Serbia
3Serbian Chamber of Comerce, BelgradeBelgrade, Serbia
Abstract
Knežević D., V. Maklenović, Lj. Kolarić, D. Mićanović, A. Šekularac and J. Knežević (2016): Variation and inheritance of nitrogen content in seed of wheat genotypes (Triticum aestivum L.) – Genetika, vol. 48, no2, 579-586
In diallel crosses (without reciprocals) of four genetically divergent wheat cultivars (Jugoslavija, Osiječanka, Žitnica, and NS Rana 2) produced seed of hybrids which planted on experimental field. F2 hybrids plants used for harvest and source of F3 seeds which used for nitrogen content analysis. The mode of inheritance, gene effect, heritability in parent cultivars and F3 hybrids were studied for nitrogen content in seed. On the base of obtained results, different mode of inheritance: dominance, intermediate and overdominance for content of N in seed was established. Among parent cultivars, the highest nitrogen contents had Novosadska Rana 2 (3.50%). The combination Osiječanka/NS Rana 2 in F3 grain generation was the best for nitrogen content (3.70%).
Key words: cultivars, hybrids, inheritance, nitrogen, wheat
Corresponding author: Desimir Knežević-Faculty of Agriculture, University of Priština, Kosovska Mitrovica, Lesak 38219, Kopaonicka bb., Kosovo and Metohija, Serbia, Phone: +381 64 614 8882 e-mail deskoa@ptt.rs
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp. 587 -596
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
MUTATION IN SECOND EXON OF MYO15A GENE CAUSE OF NONSYNDROMIC HEARING LOSS AND ITS ASSOCIATION IN THE ARAB POPULATION IN IRAN
Samira ASGHARZADE1, Morteza HASHEMZADE CHALESHTORI2, Mohammad Amin TABATABAIFAR3, Somayeh REISI 4, Mohammad Hossein MODARESSI1
1Department of Molecular Medicine School of advanced Medical Technologies, Tehran University of Medical Science, Tehran, Iran
2Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Genetics, Faculty of Basic Sciences, University of Shahrekord, Iran
Abstract
Asgharzade S., M. H. Chaleshtori, M. A.Tabatabaifar, S. Reisi , M. H. Modaress (2016):Mutation in second exon of myo15a gene cause of nonsyndromic hearing loss and its association in the Arab population in Iran.- Genetika, Vol 48, No.2,587 -596.
Hearing loss is a genetically and clinically heterogeneous defect and more than 140 loci and 65 genes have been identified to cause autosomal recessive non-syndromic hearing loss (ARNSHL). According to the previous studies, mutations in GJB2 are estimated to be involved in 18.17% of ARNSHL cases in the Iranian population; as a result, the remaining 81.83% of this disorder is yet ambiguous. This study aimed to determine the contribution of DFNB3 in hearing loss as well as the frequency of gene mutations in a population (Arab tribal origin) in the Southwest of Iran.
In this descriptive laboratory study, we included 25 families from the Southwest of Iran and negative GJB2 gene. Linkage analysis was performed by DFNB3 (MYO15A) molecular markers (STR). The families with hearing loss linked to this locus were further analyzed for mutation detection. MYO15A gene exons were amplified and analyzed using direct DNA sequencing.
In studied families, one family displayed linkage to DFNB3 locus. Identified mutations include substitution and substitute C for A in 1047 location of coding region of MYO15A gene (c.1047 C>A) in exon 2 which cause to change Tyrosin to stop codons (P.Y349X), results in the premature truncation at amino acid position 349.
Key words: MYO15A, hearing loss, linkage analysis, Iran
Corresponding author: Mohammad Hossein Modaressi,Molecular Medicine Departments, Tehran University of Medical Sciences,Tehran, Iran. 14155-6447, P. O. Box: 147789-3855,Telefax: +98(21)88953005 / +98(21)44865239
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 597-607
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
Original scientific paper
ANALYSIS AND COMPARISON OF FRAGRANT GENE SEQUENCE IN SOME RICE CULTIVARS
Noushafarin KARAMIA, Ali AALAMIB, Habibollah Samizadeh LAHIJIC,
Babak RABIEID and Mehrzad ALAHGHOLIPOURE
a,b,c and d Department of Agronomy and Plant Breeding, Faculty of Agricultural sciences,
University of Guilan, Rasht, Iran
e Department of Plant Breeding, Rice Research Institute of Iran (RRII), Rasht/Iran
2Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Karami N., A. Aalami, H. S. Lahiji, B. Rabiei and M. Alahgholipour (2016): Analysis and comparison of fragrant gene sequence in some rice cultivars.-Genetika, Vol 48, No. 2,597-607.
It is known that the fragrant trait in rice (Oryza sativa L.) is largely controlled by fgr gene on chromosome 8 and it has been specified that the existence of an 8 bp deletion and three single nucleotide polymorphism (SNP) in exon 7 is effective on this trait. In this study, sequence alignment analysis of fgr exon7 on chromosome 8 for 11 different fragrant and non-fragrant cultivars revealed that 5 aromatic rice cultivars carried 3 SNPs and 8 bp deletion in exon7 which terminates prematurely at a TAA stop codon. However, 5 of the non-aromatics showed a sequence identical to the published Nipponbare, being non-fragrant Japonica variety sequence. An exception among them was Bejar, which had 8 bp deletion and 3SNPs but it was non-aromatic. Sequencing can determine nucleotide alignment of a gene and give beneficial information about gene function. In silico prediction showed proteins sequences alignment of fgr gene for Khazar and Domsiah genotypes were different. Betaine aldehyde dehydrogenase complete enzyme belongs to Khazar non-fragrant genotype that has complete length and 503 amino acids while non-functional BADH2 enzyme for Domsiah fragrant genotype has 251 amino acids that result in accumulate 2-acetyl-1-pyrroline (2AP) and produces aroma in fragrant genotypes.
Key words: 2-acetyl-1-pyrroline (2AP), Betaine aldehyde dehydrogenase (BADH2), Exon 7, Fgr gene, Sequencing
Corresponding author: Ali Aalami, Department of Agronomy and Plant Breeding, Faculty of Agricultural sciences, University of Guilan, Iran. P.O. Box: 41635-1314/ Rasht/ Iran, E-mail address: ali_aalami@guilan.ac.ir
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp. 609- 616
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
THE FREQUENCIES OF FV LEIDEN AND FII G20210A MUTATIONS IN PATIENTS WITH DIFFERENT CLINICAL MANIFESTATIONS OF VENOUS THROMBOEMBOLISM: EXPERIENCE FROM LARGE SERBIAN COHORT
Branko tomic1*, Maja gvozdenov1, Iva pruner1, Mirjana kovac2,3,
Nebojsa antonijevic2,4, Dragica radojkovic 1, Valentina djordjevic1
1Institut of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia
2Faculty of Medicine, University of Belgrade, Serbia
3Blood Transfusion Institute of Serbia, Hemostasis Department, Belgrade, Serbia
4Clinic for Cardiology, Clinical Center of Serbia, Belgrade, Serbia
Abstract
Tomic B., M. Gvozdenov, I. Pruner, M. Kovac, N. Antonijevic, D. Radojkovic, V. Djordjevic (2016). The frequencies of FV leiden and FII G20210a mutations in patients with different clinical manifestations of venous thromboembolism: experience from large Serbian cohort--Genetika, Vol 48, No. 2, 609 -616.
Venous thromboembolism is a multifactorial disorder with two manifestations: deep-vein thrombosis and pulmonary embolism. Pulmonary embolism is usually considered as the complication of deep-vein thrombosis, but there are reported cases of isolated pulmonary embolism. FV Leiden and FII G20210A mutations are most common genetic risk factors for the venous thromboembolism. Several studies reported "FV Leiden paradox": lower prevalence of FV Leiden mutation among patients with isolated pulmonary embolism than among those with deep-vein thrombosis. The aim of this study was to determine FV Leiden and FII G20210A mutations frequency in thrombophilic patients in Serbian population. We tested prevalence of these mutations carriers in 1427 individuals divided in three groups of patients (with deep-vein thrombosis, deep-vein thrombosis/ pulmonary embolism and isolated pulmonary embolism) and control group. All subjects were tested for these mutations using PCR-RFLP analysis. Detected frequency of FV Leiden heterozygous carriers in patients with isolated pulmonary embolism was 6.9% (for FII G20210A 11.6%), while in other two groups of patients with deep-vein thrombosis and deep vein thrombosis/pulmonary embolism, frequency was 18.6% (for FII G20210A mutation were 11.6% and 8.3%, respectively). Our results showed that FV Leiden mutation is less frequent in patients with isolated pulmonary embolism than in patients with deep-vein thrombosis or deep-vein thrombosis accompanied with pulmonary embolism, confirming "FV Leiden paradox". On the other hand, detected frequency of FII G20210A mutation carriers was similar in all three groups of patients.
Keywords: deep-vein thrombosis; pulmonary embolism; venous thromboembolism; FII G20210A; FV Leiden; paradox
Corresponding author: Tomic Branko, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010 Belgrade, Serbia, Tel: +38113976658,Fax: +381113975808,e-mail: kobran@imgge.bg.ac.rs
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 617- 627
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
EFFECTS OF PHOTOPOLYMERISATION ON GENOTOXICITY OF COMPOSITE ADHESIVES IN THE COMET ASSAY
Stefan dačić1, Ninoslav djelić2, Milena radaković2, Nada lakić3, Aleksandar veselinović4, Marko ristanić2, Marko andjelković5
1Department for Restorative Dentistry and Endodontics, Clinic of Dentistry, Faculty of Medicine, University of Niš, Niš, Serbia
2Department of Biology, Faculty of Veterinary Medicine, Univesity of Belgrade, Belgrade, Serbia
3 Department of Statistics, Faculty of Agriculture, University of Belgrade
4Department of Chemistry, Facutly of Medicine, University of Niš, Niš, Serbia
5Serbian Academy of Sciences and Arts, Belgrade, Serbia
Abstract
dačić S., N. djelić, M. radaković, N. lakić, A. veselinović, M. ristanić, M. Andjelković (2016): Effects of photopolymerisation on genotoxicity of composite adhesives in the Comet assay.- Genetika, Vol 48, No. 2, 617-627.
Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 µg/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, χ2 test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.
Keywords: composite adhesives, photopolymerisation, genotoxicity, Comet asssay
Corresponding author: Ninoslav Djelić, Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18,11000 Belgrade, Serbia,Phone: +381 11 265 88 94,Fax: +381 11 268 59 36,E-mail: ndjelic@vet.bg.ac.rs
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 629-642
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
COMPARISON OF REAL-TIME PCR PROTOCOLS IN DETECTION AND QUANTIFICATION OF FRUIT TREE 16SRX GROUP PHYTOPLASMAS
Tomas kiss*, Tomas necas, Jana necasova
Department of Fruit Growing, Faculty of Horticulture in Lednice, Mendel University in Brno, Czech Republic
Abstract
Kiss T., T. Necas, J. Necasova (2016). Comparison of real-time pcr protocols in detection and quantification of fruit tree 16srx group phytoplasmas- Genetika, Vol 48, No.2,629 -642.
In this work, two real-time PCR protocols based on intercalating dye and two on hydrolysis probes were tested using field collected fruit tree samples infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and sensitivity of protocols and amplification efficiency were the main testing parameters. Results of real-time PCR protocols were compared to nested PCR. All real-time PCR protocols confirmed their specificity of detection. All real-time PCR protocols were 10-100 times more sensitive than nested PCR. Afterall real-time PCR protocols based on hydrolysis probes were 10 times more sensitive than protocols based on intercalating dyes. Among protocols based on hydrolysis probes, slightly better detection characteristics were shown by protocol by christensen et al. (2004).
Keywords: AP; Bryt Green; ESFY; PD; Malus; Prunus; Pyrus; real-time PCR; TaqMan; TaqMan MGB
Corresponding author: Tomas kiss, Department of Fruit Growing, Faculty of Horticulture in Lednice, MENDELU, Valtická 337, Lednice, 691 44, Czech Republic, email address: xkiss@mendelu.cz
Published in „GENETIKA“ Vol. 48, No. 2 (2016), pp. 643-652
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633
Original scientific paper
IDENTIFICATION OF QUANTITATIVE TRAIT LOCI FOR LEAF TRAITS IN RICE
Baoyan jia1,2§, Xinhua zhao1,2§, Yang qin1, Muhammad irfan3, Tae-heon kim1, Bolun wang1, Shu wang1, Jae Keun sohn2*
1 Department of Agronomy, Shenyang Agricultural University, Shenyang China
2 Department of Agronomy, Kyungpook National University, Daegu, Republic of Korea
3 Bioscience & Biotechnology college, Shenyang Agricultural University, Shenyang China
Abstract
jia B., X. zhao, Y. qin, M. irfan, T.-H. kim, B. wang, S. wang, J. K. sohn (2016). Identification of quantitative trait loci for leaf traits in rice. - Genetika, Vol 48, No. 2,643 -652.
A recombinant inbred lines (RILs) population of 90 lines were developed from a subspecies cross between an indica type cultivar, ‘Cheongcheong’, and a japonica rice cultivar, ‘Nagdong’ was evaluated for leaf traits in 2009. A genetic linkage map consisting of 154 simple sequence repeat (SSR) markers was constructed, covering 1973.6 cM of 12 chromosomes with an average map distance of 13.9 cM between markers. By composite interval mapping method a total of 19 QTLs were identified for the leaf traits on 5 chromosomes (Chr.1, Chr.3, Chr.6, Chr.8 and Chr.11). The percentage of phenotypic variance explained by each QTL varied from 8.1% to 29.4%. Five pleiotropic effects loci were identified on chromosomes 1, 6.
Key words: Leaf trait, QTL, SSR, Rice
Corresponding author: Baoyan jia, Department of Agronomy, Shenyang Agricultural University, 110161, Shenyang China
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 653-663
© 2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
INTER POPULATION VARIABILITY OF FROST-RESISTANCE IN
PROVENANCES OF SCOT PINES (Pinusylvestris L. R. hamata Steven) IN
TURKEY
*Halil Barış ÖZEL1, Sezgin AYAN2, Vasilije ISAJEV3
1 University of Bartın, Faculty of Forestry, Department of Silviculture, Bartın,Turkey
2 University of Kastamonu, Faculty of Forestry, Department of Silviculture, Kastamonu,Turkey
3 Institute for Forestry, Belgrade,Serbia
Abstract
Özel B. H., S.Ayan, V. Isajev (2016): Inter population variability of frost-resistance in provenances of scot pines (Pinusylvestris l. R. hamata Steven) in Turkey..- Genetika, vol 48 no. 2, 653-663.
Frost-resistance variability of Scotch pine (Pinus sylvestris L. var. hamata Steven) seedlings grown in nurseries conditions, originated from 10 provenances, have been analyzed. The provenances from Black Sea region, Central Anatolian region and Eastern Anatolian region in Turkey have been used in selection of seed zones. The results of frost-resistance tests indicated a strong relationship of implemented freezing degrees with injury degrees of Scotch pine needles and photosynthetic productivities. On the other hand, another significant relationship has been determined between chlorophyll fluorescence and ion leakage methods (r=-0.801). This result shows that those two methods can be safely used in determining the damages due to low temperatures. In frost resistance tests, Scotch pine seedlings from different provenances have been frozen at -10, -20, -30 and -40 °Ϲ. According to the Duncan test results, it has been determined that damage increased as temperature decreased. The damage level at -10°Ϲ implementation is 3.5% which can be tolerated by plants. But when the temperature has been decreased to -20°Ϲ, the level of damage has increased to 51.25%. As a result of photosynthetic analyses in this phase, it has been determined that there is a statistically significant relationship between provenances and temperature levels. Under the light of those findings, they have determined that the photosynthetic productivity has significantly decreased at temperatures between -20°Ϲ and -40°Ϲ. This situation conforms to injury index values determined in this study. As a result of injury index and photosynthetic productivity tests used for determining the damage after frost-resistance tests, it has been determined that the provenances of Amasya-Kunduz, Bolu-Aladağ, Düzce-Yığılca, Samsun-Vezirköprü and Eskişehir-Çatacık are more sensitive to frost than other provenances.
Keywords: Scotch pine, provenances, frost damage, injury index, photosynthetic activity.
Corresponding author: Halil Barış ÖZEL, Bartın University, Faculty of Forestry, Department of Silviculture, Agdacı Campus, 74100 Bartın-TURKEY, Phone:090 3782235153, Fax: 090 3782235062, e-mail: halilbarisozel@yahoo.com
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 665-674
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
SESAME GERMPLASM EVALUATION FOR REPRODUCTIVE PERIOD AND HARVEST INDEX
Bachubhai Arjanbhai monpara
Agricultural Research Station, Junagadh Agricultural University, Keriya Road, Amreli (Gujarat), India
Abstract
monpara B. A.(2016):Sesame germplasm evaluation for reproductive period and harvest index.Genetika vol48, no2, 665-.674
In sesame, reproductive period is a component of crop maturity and harvest index is a component of seed yield. Both are important traits in applied plant breeding and associated with seed filling process, which helps optimizing crop duration and maximizing sesame yield. The study was focused on genetic control of these traits and to identify potential genotypes to use as parents in breeding programme. Seventy sesame germplasm lines were evaluated in randomized block design replicated thrice. Great variability for reproductive period and harvest index was observed. High heritability accompanied by high genetic gain for reproductive period, harvest index, biological yield and seed yield. Reproductive period was associated positively with days to maturity. Harvest index was associated positively with seed yield and biological yield but negatively with days to maturity. These circumstances suggest that increasing seed yield in sesame is possible through breeding for reproductive period and harvest index. However, performance data of selected genotypes demonstrate that it is wise to select for yield in conjunction with high biological yield and greater proportion of photosynthate partitioning together with optimum length of reproductive period. Use of present genetic variation like MARGO-TALL in manipulating both reproductive period and harvest index may be beneficial in sesame breeding programme.
Keywords: Sesamum indicum, germplasm, physiological traits, character association, yield
Corresponding author: Bachubhai Arjanbhai monpara, Agricultural Research Station, Junagadh Agricultural University, Keriya Road, Amreli- 365601(Gujarat), India. E-mail: bamonpara@yahoo.com
Published in „GENETIKA“ Vol. 48, No.2 (2016), pp. 675-690
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
Original scientific paper
BIPLOT ANALYSIS OF TRAIT RELATIONS OF SPINACH (Spinacia oleracea L.) LANDRACES
Naser sabaghnia1*, Mehdi mohebodini, Mohsen janmohammadi1
1Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.
Department of Horticulture Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Iran
Abstract
sabaghnia N., M mohebodini, M.janmohammadi (2016): Biplot analysis of trait relations of spinach (Spinacia oleracea L.) landraces .-Genetika, Vol 48, No. 2, 675 -690.
Interest in growing winter spinach (Spinacia oleracea L.) in Iran is increasing due to its good nutritional potential returns relative to other vegetable crops. The objectives of this research were to investigate the interrelationships among different traits of spinach and to evaluate different Iranian spinach landraces with application of the genotype × trait (GT) biplot methodology in visualizing research data. 81 spinach landraces were grown during 2-years according to randomized complete block design with four replications. Ranking of the genotypes based on the ideal entry revealed that genotypes G1, G20, G7, G8, G9, G27, G49 G70 and G79 were higher in the measured traits and could be good candidates for improving most of the measured traits. Ranking of traits for the leaf yield showed that petiole diameter, petiole length, leaf numbers at flowering, 1000-seed weight and root dry weight were the most discriminating traits which influence spinach leaf yield at both years. There were 9 winning genotypes and 4 which-won-where patterns at the first year while there were 8 winning genotypes and 4 which-won-where patterns at the second year. As a result, the findings from our study are as follows: (i) traits leaf numbers at flowering, leaf length, leaf width, leaf area, petiole diameter and petiole length could be as selection indices for spinach leaf yield improvement, (ii) genotypes G1, G20, G7, G8, G9, G27, G49 G70, and G79 were the most favorable and is thus recommended for commercial release or incorporating in breeding programs; (iii) the GT biplot method can be used to identify superior genotypes in other crops and in other parts of the world.
Keywords: correlation, genotype × trait biplot, principal component analysis
Corresponding author: Naser sabaghnia,Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh,,Iran, sabaghnia@maragheh.ac.ir
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 691-698
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
Original scientific paper
THE APPLICATION OF PROTEIN MARKERS IN CONVERSION OF MAIZE INBRED LINES TO THE CYTOPLASMIC MALE STERILITY BASIS
Milan stevanovic*, Zoran camdzija, Jovan pavlov, Ksenija markovic,
Jelena vancetovic, Snezana mladenovic drinic, Milomir filipovic
Maize Research Institute Zemun Polje, Zemun Polje, Serbia
Abstract
stevanovic M., Z. camdzija, J.pavlov, K. markovic, J. vancetovic, .S. mladenovic drinic, M. filipovic (2016): The application of protein markers in conversion of maize inbred lines to the cytoplasmic male sterility basis.- Genetika vol 48, no2, 691-698.
A total of seven maize inbred lines of different origin and maturity group were used in the trial set up according to the split-plot randomized complete block design in five environments. Each inbred was observed in five variants: original inbred (N); cytoplasmic male sterile C-type (CMS-C); restorer for CMS-C (RfC); cytoplasmic male sterile S-type (CMS-S) and restorer for CMS-S (RfS). The objective was to compare grain yield of original inbreds and their CMS and Rf variants and to apply Isoelectric focusing (IEF) to determine whether the conversion of original inbreds to their CMS and Rf counterparts have been done completely. Protein markers have shown that conversion of almost all inbreds was done good and completely. Only original inbreds ZPL2 and ZPL5 did not concur on banding patterns with their RfC variants. The type of cytoplasm had a very significant impact on grain yield. Namely, CMS-C counterparts significantly out yielded their CMS-S versions, while the inbreds with C and S cytoplasm over yielded inbreds with N cytoplasm, as well as their RfC and RfS versions.
Keywords: banding patterns, grain yield, isoelectric focusing (IEF), male sterility
Corresponding author: Milan Stevanovic,Maize Research Institute Zemun Polje, Slobodana Bajica 1, 11185 Zemun Polje, Serbia ,Phone: +381648406046, Fax: +381113756707 ; e-mail: mstevanovic@mrizp.rs
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 699-706
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
Original scientific paper
Difference in apolipoprotein E genotype distribution between dentate and edentulous elderly patients with Alzheimer disease
Aleksandra POPOVAC1, Ivica STANČIĆ1, Nebojša DESPOTOVIĆ2, Nađa NIKOLIĆ3, Elka STEFANOVA4, Jelena MILAŠIN3
1 Clinic for Prosthodontics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia
2 Geriatric Institute Clinical Hospital Centre Zvezdara, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
3 Department of Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia
4 Clinic for Neurology, Faculty of Medicine, Clinical Hospital Centre Belgrade, University of Belgrade, Belgrade, Serbia
.
Abstract
Popovac A., .Stančić, N.Despotović, N. Nikolić, E. Stefanova J. Milašin (2016): Difference in apolipoprotein e genotype distribution between dentate and edentulous elderly patients with Alzheimer diseaseGenetika, Vol 48, No2, 699-706.
Association between dementia and tooth loss has been shown although the nature of that association is not clear. It has also been shown that risk of dementia was increased in apolipoprotein E4 allele carriers. The objective of this study was to determine the frequency of APOE alleles and their association with the dental status in elderly demented patients. Dental status of 67 patients with dementia was recorded. DNA was isolated from buccal swabs and genotyping was done by PCR-RFLP. The majority of participants had E3/E4 genotype (55.2%) and these heterozygotes were significantly more frequent than any other genotype (p<0.001). There was no significant association between dental status and genotype. However, partial edentulousness with very few teeth in both jaws (1-9 teeth) was significantly more frequent among demented patients with E3/E4 genotype (p=0.021). Patients with Alzheimer disease most frequently had E3/E4 genotype and had very few or no teeth.
Key words: Alzheimer disease, apolipoprotein E polymorphism, teeth number, dental status.
Corresponding author: Jelena Milašin,Dr Subotića 8,Mail: jelena.milasin@stomf.bg.ac.rs,Tel. +381637596550
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 707-716
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
ANALYSIS OF T-786C And 4a/b ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE POLYMORPHISMS IN RETINOPATHY OF PREMATURITY
Jelica, R. PANTELIĆ1, Tatjana, J. VARLJEN 2, Nela, S MAKSIMOVIĆ 3, Biljana, B. JEKIĆ3, Ana, J. OROS 4, Tatjana, V. NIKOLIĆ 5, Ivan, B. STEFANOVIĆ1,6, Ivana, V. NOVAKOVIĆ3 and Tatjana, M. DAMNJANOVIĆ3
1Clinic for Eye Diseases, Clinical Center of Serbia, 2 Pasterova str., Belgrade, Serbia
2Institute of Legal Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
3Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
4Clinic for Eye Diseases, Clinical Centre of Vojvodina, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbiaisegradska Str Belgrade, Serbia
6Faculty of Medicine, University of Belgrade, r Belgrade, Serbia
Abstract
Pantelić .J., T. Varljen, N. Maksimović, B.. Jekić, A.. Oros , T. Nikolić, I. Stefanović, I. Novaković and T.. Damnjanović (2016): Analysis of t-786c and 4a/b endothelial nitric oxide synthase gene polymorphisms in retinopathy of prematurity.- Genetika vol 48, no2, 707-716.
Retinopathy of prematurity (ROP) is a vascular proliferative disorder of retina, that causes visual impairment in premature children. Beside well known risk factors such as short gestational age, low birth weight and early oxygen exposure, genetic susceptibility is considered as a risk factor for development of the disease. The aim of our study was to explore the association of T-786C and 4a/b eNOS gene polymorphisms with the development of severe ROP. Study included 174 preterm infants, 84 with ROP and 90 as a control group. No differences have been observed in genotypes and alleles distributions of eNOS T-786C and eNOS 4a/b polymorphisms between two analyzed groups. There was significant difference in female infants by dominant model for 4a/b genotypes (4bb/4ba+4aa). Namely, female infants in ROP group were more frequently carriers of 4ba and 4aa genotypes than female infants in control group (p=0.037). Analysis of association between 4a/b eNOS polymorphism and ROP among preterm infants have not shown statistically significant association (p=0.288). Gestational age values by recessive model (4bb+4ba/4aa) were significantly lower in infants with 4aa genotype (t=2.034 p=0.044). Almost all detected 4aa genotypes were present in the group of infants with gestational age under 30 weeks (p=0.032), but multivariate linear regression analysis does not show association of 4a/b genotypes with gestational age of premature infants. According to results of the present study T-786C and 4a/b polymorphisms of the eNOS gene may not be the risk factors for the manifestation of severe ROP in Serbian infants.
Key words: T-786C polymorphism, 4a/b polymorphism, eNOS gene, SNP, retinopathy of prematurity.
Corresponding author: Tatjana Damnjanovic, Institute of Human Genetics, Faculty of Medicine, University of Belgrade, 26 Visegradska Str., Belgrade, Serbi,Email:tatjanadamnjanovic@yahoo.com,Tel: +381 11 3607051, Fax: +381 11 3607042, Mob: +381 64 2189641
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp 717-727
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
YIELD AND YIELD TRAITS OF DURUM WHEAT (Triticum durum desf.) AND BREAD WHEAT (Triticum aestivum L.) GENOTYPES UNDER DROUGHT STRESS
Tofig ALLAHVERDIYEV
Research Institute of Crop Husbandry Ministry of Agriculture, Baku, Azerbaijan
Abstract
Allahverdiyev T. (2016): Yield and yield traits of durum wheat (Triticum durum desf.) and bread wheat (Triticum Aestivum L.) genotypes under drought stress- Genetika, Vol 48, No. 2, 717 - 727.
Field experiment was conducted to study the effect of water stress on yield and yield traits of durum wheat and bread wheat genotypes. Water stress caused significant reduction in plant height (PH), peduncle length (PL), spike number/m2(SN), spike length (SL), spike width (SW), spikelets number/spike (SNS), spike mass (SM), grain number/spike (GNS), grain mass/spike (GMS), biological yield (BY), thousand kernel mass (TKM), grain yield (GY) and harvest index (HI). Wheat traits such as SN, SM, BY, TKM, GY were more vulnerable to drought stress. Positive significant correlation of GY with SN, BY and HI under rain-fed condition was found. Genotypes of durum wheat were more sensitive to drought than that bread wheat genotypes. The significant and positive correlation of GY with Stress Tolerance Index (STI), Mean Productivity (MP) and Geometric Mean Productivity (GMP) indicated that these indices were more effective in identifying high yielding, drought tolerance genotypes.
Key words: bread wheat, drought stress, durum wheat, genotypes, yield traits
Corresponding author: Tofig Allahverdiyev, Research Institute of Crop Husbandry Ministry of Agriculture, Baku, Azerbaijan, e-mail: tofig_1968@mail.ru
Published in „GENETIKA“ Vol. 48, No. 2(2015), pp. 729-742
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.630
Original scientific paper
Genetic diversity of common bean accessions from Former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers
Marko MARAS1, Afrodita Ibusoska2, Suzana Kratovalieva2, Rukie Agić3, Jelka Šuštar-Vozlič1, Vladimir Meglič1
1Agricultural Institute of Slovenia, Ljubljana, Slovenia
2Institute of Agriculture, Skopje, FYR of Macedonia
3Faculty of Agricultural Sciences and Food, Skopje, FYR of Macedonia
Abstract
Maras M., A. Ibusoska, S. Kratovalieva, R. Agić, J. Šuštar-Vozlič, V. Meglič (2016): Genetic diversity of common bean accessions from Former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers- Genetika, Vol 48, No. 2, 729-742.
Cultivation of common bean has a long tradition in the Former Yugoslav Republic of Macedonia (FYROM) and is still nowadays important part of the human diet. In a study reported here 71 accessions from the FYROM were assessed for genetic diversity with the aim to provide information on genetic structure of Macedonian common bean germplasm and to depict its peculiarities. A total of 71 accessions were assessed using 13 microsatellite and 16 morphological markers. The average number of alleles per microsatellite was 5.8, and ranged from three to 16 alleles. High capacity of selected markers for distinguishing genotypes was identified by the calculation of a very low value of probability of identity. The relationship among 71 studied accessions was assessed by hierarchical cluster analysis. A very clear separation of accessions into two groups was observed in the UPGMA dendrogram. The larger represented Andean gene pool and contained 40 accessions (56% of total), while the other 31 accessions (44% of total) composed Mesoamerican gene pool. The two groups were successfully discriminated by eight morphological traits. Within the larger Andean cluster in the UPGMA dendrogram a sub-group of 16 climbing accessions was separated from 24 bush accessions. The absence of the string in the pods of the climbers suggests that this sub-group comprises snap beans grown primarily for their fresh pods. There were eight morphological traits in total that distinguished the two Andean sub-groups. Assessment of genetic relationship among accessions, their classification into respective gene pool and identification of morphological peculiarities provided valuable information for the management of plant gene bank and Macedonian bean breeding program.
Key words: Phaseolus vulgaris L., landraces, diversity, microsatellite markers, morphological traits
Corresponding author: Marko Maras, Agricultural Institute of Slovenia, Ljubljana, Slovenia, phone: +38612805 282; fax number: +38612805255; e-mail address: marko.maras@kis.si
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 743-751
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
ANTHROPOGENETIC VARIABILITY IN GROUPS OF CHILDREN FROM REGULAR AND SPECIAL SCHOOLS FROM DIFFERENT LOCALITIES IN SERBIA
Slavko BRANKOVIĆ1 and Suzana CVJETIĆANIN2
1 Faculty of Natural and Mathematics Science, University in Priština with the settlement in Kosovska Mitrovica, Serbia
2 Institute of Human Genetics, School of Medicine, University of Belgrade
Abstract
Branković S. and S. Cvjetićanin (2016):Anthropogenetic variability in groups of children from regular and special schools from different localities in Serbia. Genetika, Vol 48, No.2, 743 -751.
This population-genetic study compares morpholophysiological and genetic variability in five control groups of individuals (children from five regular schools, N= 996) with children from that many special schools (N= 736) from Serbia, by using a test of determination of homozygously recessive characteristics in humans (HRC-test). Genetic homozygosity degree showed not only statistically significant difference between the mean values obtained for two groups of studied samples (control group 6.95± 0.07; children from special schools 8.63± 0.08 HRCs, out of 30 analyzed characteristics), but also differences in the type of distribution, as well as the presence of specific combinations of such traits. Results of comparisons done in different places (Kraljevo, Nis, Vranje, Leskovac, Pirot) showed the same tendency- the increase of genetic homozygosity and relative decrease of variability in samples of children from special schools. The number of HRCs among individuals from control groups varied from 2 to 15/30, and from 3 to 16/30 among children from special schools. It is possible that increased recessive homozygosity present in the group of children from special schools leads to increase of genetic loads, what may cause easier expression of some physiological and mental abilities that children from special schools have.A great individual variation in amount of genetic homozygosity that exists among human individuals may influence their potentials for different kinds of adaptation, including their mental abilities, physical capacities or resistance to different diseases.
Key words: genetic homozygosity, anthropogenetic variability, school children
Corresponding author: Suzana Cvjeticanin, Institute of Human Genetics, School of Medicine, University of Belgrade Visegradska 26, 11000 Belgrade, Serbia,Tel +381 60 3534821 , Tel/Fax. +381 11 3607040,E-mail: cujasimsi@gmail.com
Published in „GENETIKA“ Vol. 48, No. 2(2016), pp. 753-760
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
Familly with two different cases of post- and pre-natal l1 syndrome; when hydrocephaly become “multidisciplinary headache”
Nenad bukvic1, Francesca boaretto2, Giuseppe loverro3, Francesco c. susca4, Rosaura lovaglio4, Margherita patruno4, Dragoslav bukvic5, Srdjan starcevic6, Giovanni vazza2, Maria Luisa mostaciuollo2, Nicoletta resta4
1University Hospital Policlinics of Bari, Medical Genetics, Bari, Italy
2University of Padua, Department of Biology-Laboratory of Human Genetics, Padua, Italy
3University of Bari, Department of Gynecology and Obstetrics, Bari, Italy
4University of Bari, DIMO-Laboratory of Medical Genetics, Bari, Italy
5Niksic General Hospital - Department of Gynecology, Niksic, Montenegro
6Clinic for Ortopedic Surgery and Traumatology, MMA(VMA), Belgrade, Serbia
Abstract
Bukvić N., F. Boaretto, G. Loverro, F. c. Susca, R. Lovaglio, M. Patruno, D. Bukvic, S.Starcevic, G. Vazza, M. L. Mostaciuollo, N. Resta (2016): Familly with two different cases of post- and pre-natal L1 syndrome; When hydrocephaly become “multidisciplinary headache”.- Genetika, Vol 48, No.2, 753 -760.
In middle 90’s the scientific community classified as CRASH syndrome a clinical situation characterized by Corpus callosum hypoplasia, Retardation, Adducted thumbs, Spastic paraplegia, and Hydrocephalus. This pathology is also known as L1 syndrome and includes a spectrum of related neurological disorders with an X-linked recessive mode of inheritance associated to mutations in the human L1 Cell Adhesion Molecule gene (L1CAM;OMIM 308840). Here we report regarding a couple pass through our Genetic Counseling, during clinical diagnostic procedure in ~3 years old son, due to presence of multiple malformations such as hydrocephalus, agenesis of corpus callosum, tetraparesys, axial hypotony, cognitive and motor incompetency. The proband was the first male child of a healthy, non-consanguineous Italian couple with no family history of brain abnormalities, recurrent miscarriages, other birth defects and/or genetic illnesses. During Genetic Counseling, a diagnostic hypothesis of L1 syndrome was made, with in deep explanation of genetic testing possibilities. On the basis of our protocol, we fixed another appointment 2 weeks later, but unfortunately the family never showed up. Approximately one year later, the Department of Gynecology and Obstetrics requested Genetic Counseling for a 33 years old woman, secondigravida (22° gestation week), with abnormal ultrasound findings showing severe fetal ventriculomegaly. The family history was unremarkable with no consanguinity; she was one of two sisters and had a healthy brother. Surprisingly the woman was the mother of our proband. On the basis of a diagnostic hypothesis of L1 syndrome (includes a spectrum of related neurological disorders with an X-linked recessive mode of inheritance), we performed molecular analysis on the proband’s DNA, mother’s DNA and fetal DNA. The mutational screening revealed the presence of a non sense c.2701C>T (p.Arg901*) mutation in the exon 20 of the L1CAM gene in all the tested DNA samples. This observation underline a necessity to consider L1-syndrome as potential part of differential diagnosis in all cases of hydrocephaly, same as undoubting of importance of genetic counseling with subsequent, extension of the molecular analysis to the relatives in order to provide an effective and reliable counseling and risk assessment to these families. Finally, the development of new analytical tools [primarily next generation sequencing (NGS)] and development of an assay/gene panel can be used to specifically target a L1 Syndrome for fast, reliable and high quality results in tricky clinical situation like this one.
Keywords: Hydrocephaly, L1 syndrome, L1CAM gene, pre- and post-natal diagnosis
Corresponding author: Prof. Nenad Bukvic, MD, PhD, Specialist of Medical Genetics, University Hospital Policlinics of Bari, Medical Genetics Unit, Piazza Giulio Cesare, 11, 70125 Bari, Italy. e-mail: nenad.bukvic@policlinico.ba.it; nenadbukvic@virgilio.it. Tel: +39 080 5593621.
Published in „GENETIKA“ Vol. 48 No. 2(2016), pp. 761-776
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
ZERO TILLAGE: A POTENTIAL TECHNOLOGY TO IMPROVE COTTON YIELD
Hafiz Ghazanfar abbas1, Abid mahmood1 and Qurban ali2
1Cotton Research Institute, Ayub Agricultural Research Institute Faisalabad, Pakistan
2Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan
Abstract
abbas H. G., A. mahmood and Q. ali (2016): Zero tillage: a potential technology to improve cotton yield.- Genetika, Vol 48, No.2, 761 -776.
Zero tillage technology revealed with no use of any soil inverting technique to grow crops. The crop plant seed is planted in the soil directly after irrigation to make the soil soft without any replenishing in soil layers. A study was conducted to evaluate cotton genotypes FH-114 and FH-142 for the consecutive three years of growing seasons from 2013-15. The seed of both genotypes was sown with two date of sowing, 1 March and 1 May of each three years of sowing under three tillage treatments (zero tillage, minimum tillage and conventional tillage) in triplicate completely randomized split-split plot design. It was found from results that significant differences were recorded for tillage treatments, date of sowing, genotypes and their interactions.
Multivariate analysis was performed to evaluate the yield and it attributed traits for potential of FH-114 and FH-142 cotton genotypes. The genotype FH-142 was found with higher and batter performance as compared to FH-114 under zero tillage, minimum tillage and conventional tillage techniques. The traits bolls per plant, boll weight, fibre fineness, fibre strength, plant height, cotton yield per plant and sympodial branches per plant were found as most contributing traits towards cotton yield and production. It was also found that FH-142 gives higher output in terms of economic gain under zero tillage with 54% increase as compared to conventional tillage technique. It was suggested that zero tillage technology should be adopted to improve cotton yield and quality. It was also recommended that further study to evaluate zero tillage as potential technology should be performed with different regions, climate and timing throughout the world.
Keywords: zero tillage, cotton, Gossypium hirsutum, multivariate analysis, cotton yield, fibre strength
Corresponding author: Qurban ali, Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan qurban.ali@cemb.edu.pk
Published in „GENETIKA“ Vol. 48, No.2(2016), pp. 777-798
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
Family-specific vs. universal PCR primers for the study of mitochondrial DNA in plants
Jelena m. Aleksić
University of Belgrade, Institute of Molecular Genetics and genetic Engineering (IMGGE), Laboratory for Plant Molecular Biology, Belgrade, Serbia
Abstract
Aleksić m. J. (2016): Family-specific vs. universal PCR primers for the study of mitochondrial DNA in plants- Genetika, Vol 48, No.2, 777-798.
Mitochondrial genomes (mtDNAs or mitogenomes) of seed plants are characterized by a notoriously unstable organization on account of which available so-called universal or consensus primers may fail to fulfil their foreseen function - amplification of various mtDNA regions in a broad range of plant taxa. Thus, the primers developed for groups assumed to have similar organization of their mitogenomes, such as families, may facilitate a broader usage of more variable non-coding portions of these genomes in group members. Using in silico PCR method and six available complete mitogenomes of Fabaceae, it has been demonstrated that only three out of 36 published universal primer and three Medicago sativa-specific primer pairs that amplify various mtDNA regions are suitable for six representatives of the Fabaceae family upon minor modifications, and develop 21 Fabaceae-specific primer pairs for amplification of all 14 cis-splicing introns in genes of NADH subunits (nad genes) which represent the most commonly used non-coding mtDNA regions in various studies in plants. Using the same method and six available complete mitogenomes of representatives of related families Cucurbitaceae, Euphorbiaceae and Rosaceae and a model plant, Arabidopsis thaliana, it has further been demonstrated that applicability of newly developed primer pairs for amplification of nad introns in more or less related taxa was dependent not only on species evolutionary distances but also on their genome sizes. A reported set of 24 primer pairs is a valuable resource which may facilitate a broader usage of mtDNA variability in future studies at both intra- and inter-specific levels in Fabaceae, which is the third largest family of flowering plants rarely studied at the mtDNA level, and in other more or less related taxa.
Keywords: Fabaceae, mitochondrion, NADH dehydrogenase, introns, PCR primers, in silico PCR method
Corresponding author: Jelena m. Aleksić, University of Belgrade, Institute of Molecular Genetics and genetic Engineering (IMGGE), Laboratory for Plant Molecular Biology, Vojvode Stepe 444a, PO box 23, 11010 Belgrade, Serbia, Tel./fax: +381 11 3976414,E-mail: aleksic_jelena@yahoo.com.au; jelena.aleksic@imgge.bg.ac.rs
Published in „GENETIKA“ Vol. 48, No.2(2016), pp. 799-799
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Genetika, 48(2), 799-799, 2016
CORRIGENDUM
Janković et al., "Wilms tumor (WT)1 gene expression in children with acute leukemia in Serbia" published in Genetika 48(1):409-421.
The authors of the above-referenced article have requested a corrigendum to it. The article should include the following correction in affiliation of authors:
2Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
Published in „GENETIKA“ Vol. 48, No.2(2016), pp. 800-800
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Genetika, 48(2),800-800, 2016
CORRIGENDUM
Ashrafi F., A.Hashemi, K. Mardani, and R. Darvishzadeh (2014): Study on genetic variability in MHC-DRB1 second exon in makuie sheep breed population. Genetika, Vol 46, No. 1, 269-275. DOI: 10.2298/GENSR1401269A
The Editor-in-Chief has been informed that the results in Table 1 presented in the article:
Study on genetic variability in MHC-DRB1 second exon in makuie sheep breed population published in the Genetika in 2014, Vol. 46, no.1 partially overlap with the results in Tables 2, published in the article Fereshteh Ashrafi, Karim Mardani, Ali Hashemi, Reza Darvishzadeh, Mohammad Farhadian Association of molecular polymorphism in exon 2 of Ovar-DRB1genewith weight traits in Iranian Makuie sheep breed, Turkish Journal of Veterinary and Animal SciencesTurk J Vet Anim Sci (2014) 38: 126-132,doi:10.3906/vet-1305-46 without proper cross-referencing.
This claim is correct and we are reporting this overlap in order to provide appropriate cross-referening to the another work.
Published in „GENETIKA“ Vol. 48, No.2(2016), pp. 801-805
©
2016 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
Genetika, 48(2), 801-805, 2016
CORRIGENDUM
Ignjatovic-Micic D., D. Ristic, V. Babic, V. Andjelkovic and J. Vancetovic (2015): Asimple SSR analysis for genetic diversity estimation of maize landraces.-Genetika, Vol 47, No. 1,53-62. DOI: 10.2298/GENSR1501053I
The Editor in Chief has been informed that a part of the data in the article published in Genetika, vol 47 (1), 2015 was published without proper cross referencing to the date already published in the paper Ignjatovic-Micic, D., D. Ristic, V. Babic, V. Andjelkovic, K. Marković J. Vancetovic (2013): Genetic assessment of maize landraces from former Yugoslavia. Genetika 45(2): 405-417.
Inspection of these data confirmed the above claim. To correct these errors the corresponding author of the article submitted a corrections. After further discussion with corresponding author and reviewers, the Editor in Chief has decided to publish a corrigendum for this article, providing correct date and correction in literature.