GENETIKA, Vol

GENETIKA, Vol. 49, No. 1 (2017)

Roshni singhand Bashisth Narayan singh

EVIDENCE FOR VARIATIONS IN THE MORPHOMETRIC TRAITS BETWEEN TWO SIBLING SPECIES OF Drosophila: D. ananassae AND D. pallidosa [Abstract] [Full text]

Ljubodrag stanisic, Vladimir dimitrijevic, Predrag simeunovic, Uros glavinic, Biljana jovanovic, Jevrosima stevanovic, Zoran stanimirovic

ASSESSMENT OF 17 MICROSATELLITE LOCI FOR THEIR USE IN PARENTAGE VERIFICATION AND INDIVIDUAL IDENTIFICATION IN THE BALKAN DONKEY BREED [Abstract] [Full text]

Saida S. sharifova, Sabina P. mehdiyeva, Mehraj A. abbasov
ANALYSIS OF GENETIC DIVERSITY AMONG DIFFERENT TOMATO GENOTYPES USING ISSR DNA MARKER [Abstract] [Full text]

Danielius Serapinas, Marius sukys, Agne BARTKEVICIUTE, Diana barkauskiene, Daiva bartkeviciene

THE SPECTRUM OF THE MOST COMMON BRCA1/BRCA2 MUTATIONS IN LITHUANIAN HIGH RISK FAMILIES [Abstract] [Full text]

Dragana ignjatovic-micic, Ana nikolic, Aleksandra nikolic, Marija kostadinovic

DNA AND BIOCHEMICAL ANALYSIS OF A POTENTIAL OPAQUE2 MAIZE POPULATION [Abstract] [Full text]

Abdulmojeed yakubu, Adebowale E. salako, Marcos de donato and Ikhide G. imumorin

APPLICATION OF COMPUTATIONAL ALGORITHMS TO ASSESS THE FUNCTIONALITY OF NON-SYNONYMOUS SUBSTITUTIONS IN MHC DRB GENE OF NIGERIAN GOATS [Abstract] [Full text]

Zeinalabedin shahadati-moghaddam, Danial kahrizi, Elham kazemi

GENETIC CHARACTERIZATION AND MOLECULAR MAPPING OF NOVEL CHLOROPHYLL DEFICIENCY GENE IN AIR-CURED TOBACCO (Nicotiana tabacum L.) [Abstract] [Full text]

Puneet kumar, Vijay Kumar singhal and Sunil Kumar srivastava

SECONDARY ASSOCIATIONS IN ‘HIMALAYAN PINK’ (Dianthus angulatus Royle ex Benth., Caryophyllaceae) FROM COLD DESERTS OF LAHAUL-SPITI [Abstract] [Full text]

Sohail ahmad jan, Zabta khan shinwari, Malik ashiq rabbani, Haris khurshid, Muhammad ishaq ibrahim, Muhammad ADIL, Muhammad ilyas

COMPARISON OF ELECTROPHORETIC PROTEIN PROFILES OF Brassica rapa SUB-SPECIES BROWN SARSON THROUGH SDS-PAGE METHOD [Abstract] [Full text]

Dušica ĆALIĆ, Ljiljana RADOJEVIĆ

HORSE CHESTNUT POLLEN QUALITY [Abstract] [Full text]

Hafsa naheed, Raziuddin, Sana abid, Quahir sohail, Ghulam hassan, Muhammad arif

HERITABILITY AND COMBINING ABILITY OF VEGETATIVE GROWTH AND PHENOLOGICAL DEVELOPMENT OF DIALLEL CROSSES OF RAPESEED [Abstract] [Full text]

Slađana MARIĆ, Sanja RADIČEVIĆ, Milan LUKIĆ, Radosav CEROVIĆ, Svetlana A. PAUNOVIĆ

DETERMINATION OF S-GENOTYPE IN APPLE AND SWEET CHERRY CULTIVARS RELEASED AT FRUIT RESEARCH INSTITUTE, ČAČAK [Abstract] [Full text]

Daniela ganeva and Nevena bogatzevska

SOURCES OF RESISTANCE TO RACES R0 AND R1 OF Pseudomonas syringae pv. tomato – AGENT OF BACTERIAL SPECK ON TOMATO [Abstract] [Full text]

Božo S. važić, Biljana S. rogić, Milanka S. drinić, Novo M. pržulj

RELATIONSHIP BETWEEN THE GENETIC hemoglobin POLYMORPHISM, morphometry and fertility of Pramenka sheep breed FROM central bosnia [Abstract] [Full text]

Velichka todorova, Ivo DJINOVIC

ASSESSMENT OF SERBIAN PEPPER VARIETIES GROWN IN CONDITIONS OF SOUTH BULGARIA [Abstract] [Full text]

Jahad SOORNI, Seyed Kamal KAZEMITABAR, Danial KAHRIZI, Ali DEHESTANI, Nadali BAGHERI

SCREENING OF CAMELINA (Camelina sativa L.) DOUBLED HAPLOID LINES FOR FREEZING TOLERANCE IN THE SEEDLING STAGE [Abstract] [Full text]

Vlado kovačević, Ivan brkić, Manda antunović, Dario iljkić, Ivana varga

GENOTYPE EFFECTS ON PHOSPHORUS, POTASSIUM, CALCIUM AND MAGNESIUM STATUS IN MAIZE [Abstract] [Full text]

Galina pevicharova and Nikolay velkov

SENSORY, CHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF Cucurbita maxima AND Cucurbita moschata GENOTYPES FROM DIFFERENT GEOGRAPHICAL ORIGINS [Abstract] [Full text]

Miłosz tkaczyk, Ivan milenković, Justyna A. nowakowska, Małgorzata borys, Tomasz kałuski, Magdalena gawlak, Michał czyż, Tomasz oszako

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF Phytophthora SPECIES ISOLATED FROM THE RHIZOSPHERE OF DECLINING OAK TREES IN KROTOSZYN PLATEAU [Abstract] [Full text]

Fatih KAHRIMAN, Mehmet ŞERMENT, Mizgin HAŞLAK, Manjit S. KANG

POLLEN EFFECT (XENIA) FOR EVALUATING BREEDING MATERIALS IN MAIZE [Abstract] [Full text]

Javad saffari-chaleshtori, Mohammad-Amin tabatabaiefar,Payam ghasemi-dehkordi, Effat farokhi, Mohammad-Taghi moradi, Morteza hashemzadeh-chaleshtori

THE LACK OF CORRELATION BETWEEN TP53 MUTATIONS AND GASTRIC CANCER: A REPORT FROM A PROVINCE OF IRAN [Abstract] [Full text]

Deyana HRISTOVA, Stoycho METODIEV, Vassil NIKOLOV, Dimitar VASSILEV, Elena TODOROVSKA

GENETIC VARIATION OF BULGARIAN AUTOCHTHONOUS SHEEP BREEDS USING MICROSATELLITE MARKERS [Abstract] [Full text]

Branislav cvjetković, Monika konnert, Barbara fussi, Milan mataruga, Mirjana šijačić-nikolić, Vanja daničić, Aleksandar lučić

NORWAY SPRUCE (Picea abies Karst.) VARIABILITY IN PROGENY TESTS IN BOSNIA AND HERZEGOVINA [Abstract] [Full text]

Hossein Astereki, Peyman Sharifi, Masoumeh Pouresmael

CORRELATION AND PATH ANALYSIS FOR GRAIN YIELD AND YIELD COMPONENTS IN CHICKPEA (Cicer arietinum L.) [Abstract] [Full text]

a. h. abu-almaaty

DETERMINATION OF SOMATIC AND SEX CHROMOSOMES OF THREE EGYPTIAN BIRDS SPECIES USING CYTOGENETIC ANALYSIS [Abstract] [Full text]

M A Gaffar al-hadi, M Rafiqul islaM, M Abdul karim, M Tofazzal islam

Morpho-physiological characterization of soybean genotypes under SUBtropical environment [Abstract] [Full text]

Mehdi joudi

GENOTYPIC VARIATIONS FOR PHOTOASSIMILATES PARTITIONING TO THE GRAINS DURING EARLY DEVELOPMENT OF ENDOSPERM IN WHEAT: ASSOCIATION WITH GRAIN WEIGHT [Abstract] [Full text]

Simranjit kaur, Salesh K. jindal, Major S. dhailwal, Neena chawla, Om Prakash meena

GENETIC DIVERSITY ANALYSIS IN ELITE LINES OF TOMATO (Solanum lycopersicum L.) FOR GROWTH, YIELD AND QUALITY PARAMETERS [Abstract] [Full text]

Dušica ademović-sazdanić, Svetlana vojvodić, S. POPOVIĆ, N. KONSTANTINIDIS

CHARACTERISATION OF B KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES AND TELOMERIC AND CENTROMERIC MOTIFS IN HEMATOPOIETIC STEM CELL TRANSPLANTATION DONORS IN VOJVODINA, SERBIA [Abstract] [Full text]

Ana OBRADOVIĆ, Slavica STANKOVIĆ, Vesna KRNJAJA, Ana NIKOLIĆ, Dragana IGNJATOVIĆ-MICIĆ, Jelena STEPANOVIĆ and Bojan DUDUK

TRICHOTHECENE CHEMOTYPE DIVERSITY OF Fusarium graminearum ISOLATED FROM WHEAT, MAIZE AND BARLEY IN SERBIA [Abstract] [Full text]

Maria SURMA, Tadeusz ADAMSKI, Zygmunt KACZMAREK, Wojciech ŚWIĘCICKI, Stanisław MEJZA, Paweł BARZYK, Anetta KUCZYŃSKA, Karolina KRYSTKOWIAK, Krzysztof MIKOŁAJACZAK, Piotr OGRODOWICZ

A MULTIVARIATE APPROACH TO THE SELECTION OF PEA (Pisum sativum L.) LINES OBTAINED BY THE SINGLE SEED DESCENT TECHNIQUE [Abstract] [Full text]

Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 1-20

UDC 575
https://doi.org/10.2298/GENSR1701001S
Orginal scientific paper

EVIDENCE FOR VARIATIONS IN THE MORPHOMETRIC TRAITS BETWEEN TWO SIBLING SPECIES OF Drosophila: D. ananassae AND D. pallidosa

Roshni singhand Bashisth Narayan singh

Genetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India

Abstract

Singh R. and B.N.Singh (2017): Evidence for variations in the morphometric traits between two sibling species of Drosophila: D. ananassae and D. pallidosa.- Genetika, Vol 49, No.1, 1-20.

Darwinian theory of evolution states that, evolution occurs through the natural selection. Therefore, demonstration of natural selection in nature is the central aim of many evolutionary studies and selection acts primarily at the phenotypic level because it is well known that phenotypic traits are the primary target of natural selection. While keeping this in view, we have studied certain morphometric traits in the sibling species pair, D. ananassae and D. pallidosa to test intra- and interspecific variations. The traits studied are wing length, thorax length, ratio of wing length and thorax length, sternopleural bristle number, ovariole number and sex-comb tooth number. In females of D. ananassae, significant strain differences were found for all the traits except ovariole number. In males, significant strain differences were found for all the traits. On the other hand, in D. pallidosa, significant strain differences were found for all the traits in both, males and females. The values of all the morphometric traits were significantly higher in females of both the species in comparison to males. The values of all the morphometric traits were higher in D. ananassae. However, the phenotypic variability, expressed in terms of coefficient of variation, was higher in D. pallidosa. Except for ratio of wing length and thorax length, CV was higher in the case of females in comparison to males. Size related traits are least variable while bristle numbers and reproductive traits are most variable. Except few, most of the traits are positively correlated with each other in both the species. Intra- and interspecific variations were found with respect to different morphometric traits. Although sibling species have been defined as morphologically identical, our results show that sibling species may show variations in certain morphometric traits and these quantitative differences in the morphometric traits act as discriminant marker between these sibling species in the lack of any qualitative differences

Keywords: D. ananassae, D. pallidosa, geographic strains, morphometric traits, sibling species

Corresponding author: Bashisth Narayan Singh, Genetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India; E-mail: bashisthsingh2004@rediffmail.com

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 21 -30

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701021S
Original scientific paper

ASSESSMENT OF 17 MICROSATELLITE LOCI FOR THEIR USE IN PARENTAGE VERIFICATION AND INDIVIDUAL IDENTIFICATION IN THE BALKAN DONKEY BREED

Ljubodrag stanisic¹*, Vladimir dimitrijevic², Predrag simeunovic³, Uros glavinic¹, Biljana jovanovic¹, Jevrosima stevanovic¹, Zoran stanimirovic¹

¹Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

²Department of Animal Breeding and Genetics, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

³Department of Farm Animal Diseases, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

Abstract

stanisic Lj., V. dimitrijevic, P. simeunovic, U. glavinic, B. jovanovic, J. stevanovic, Z. stanimirovic (2017): Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the balkan donkey breed.- Genetika, Vol 49, No.1, 21 -30.

The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion (0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.

Keywords: allele frequency, Balkan donkey, equine microsatellites, parentage testing

Corresponding author: Ljubodrag Stanisic, Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobodjenja 18, 11000 Belgrade, Serbia, Phone: +381112658894, Fax: +381112685936, Email: motoljuba@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp.31- 42

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701031S
Original scientific paper

ANALYSIS OF GENETIC DIVERSITY AMONG DIFFERENT TOMATO

GENOTYPES USING ISSR DNA MARKER

Saida S. sharifova, Sabina P. mehdiyeva, Mehraj A. abbasov

Genetic Resources Institute of Azerbaijan National Academy of Sciences,

AZ1106, Baku, Azerbaijan

Abstract

sharifova S.S., S. P. mehdiyeva, M. A. abbasov (2017): Analysis of genetic diversity among different tomato genotypes using ISSR DNA marker.- Genetika, Vol 49, No. 1, 31 - 42.

Inter simple sequence repeat (ISSR) markers were used for variation analysis among 41 tomato accessions. A total of 50 scorable bands were obtained, where 32 were polymorphic, representing 63.3% of all the amplified loci. Polymorphism percentage ranged from 50 to 90% and an average number of polymorphic bands of 4.0 were observed. An average genetic diversity index was 0.61. Primer UBC860 and UBC825 generated the greatest diversity index with a value of 0.89 and 0.85 respectively. The smallest diversity identified by primer UBC808, with an index of 0.34. The genetic similarity among studied genotypes ranged from 0.52 to 0.98. The cluster analysis based on Jaccard’s similarity coefficient divided genotypes into 6 distinct clusters on a value of 0.74. The lowest genetic distance was found between ‘Gronastiy’ and ‘AG1224’ (0.52), ‘Orange’ and ‘AG1224’ (0.54), and ‘Evgeniya’ and ‘AG1224’ (0.55) accessions. The highest similarity of 0.98 was determined between ‘Zafar’ and ‘Azerbaijan-94’, ‘Khachmaz-1’ and ‘Azerbaijan-94’, ‘Khachmaz-1’ and ‘Severyanka’, and ‘Shakar’ and ‘Absheron-1’ accessions.

Key words: allele, genetic diversity, markers, tomato

Corresponding author: Saida S. Sharifova, Genetic Resources Institute of Azerbaijan National Academy of Sciences, Azadlig Ave 155, AZ1106, Baku, Azerbaijan, phone: (00994 50) 410 82 05,e-mail: saidasharifzade@yahoo.com

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Published in „GENETIKA“ Vol. 49, No1 (2017), pp. 43 -50

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701043S
Original scientific paper

THE SPECTRUM OF THE MOST COMMON BRCA1/BRCA2 MUTATIONS IN LITHUANIAN HIGH RISK FAMILIES

Danielius Serapinas^1,2, Marius sukys¹, Agne BARTKEVICIUTE³, Diana barkauskiene⁴,

Daiva bartkeviciene³

¹Lithuanian University of Health Sciences, Medical Academy, Department of Genetics and molecular medicine, Kaunas LT-50009, Lithuania

²Mykolas Romeris university, Vilnius LT-08303, Lithuania

³Department of Obstetrics and Gynecology, Faculty of Medicine, Vilnius University, Vilnius LT-03101, Lithuania

⁴Lithuanian University of Health Sciences, Medical Academy, Department of Pulmonology and immunology, Kaunas LT-50009, Lithuania

Abstract

Serapinas D., M. sukys, A. bartkeviciute, D. barkauskiene, D. Bartkeviciene (2017): The spectrum of the most common BRCA1/BRCA2 mutations in Lithuanian high risk families.-Genetika vol49, No1., 43 - 50, 2017.

Breast cancer is the neoplasm with the highest incidence and mortality among women in Lithuania. The aim of the study was to determine the mutational incidence in BRCA1 and BRCA2 genes in high-risk breast and/or ovarian cancer families. After written informed consent, 36 participants from Lithuanian health science university hospital provided a blood sample for genetic analysis. Molecular diagnostics was done for 6 BRCA1and BRCA2 mutations. From 36 tested subjects for BRCA1/BRCA2 mutations. Positive test for BRCA1/BRCA2 mutations test was found in 12 (33%) cases. Most common BRCA1 mutation was 5328insC – 6 (50%) cases, other mutations: 185delAG - 1 (8,3%), 300t>6(c61G) - 4 (33,3%), 4153 del A – 1 (8,3%). All mutations were BRCA1, but none of the women were positive for the analyzed BRCA2 mutation. The mean age when the cancer was diagnosed in BRCA1 mutations group was 40.40±3.39 comparing with the group without mutations – 43.29 ±2.52. Rates of BRCA1 and BRCA2 mutation testing are increasing in young women with breast and ovarian cancer. Detected mutations in BRCA1 contribute to up to one-third of the families with breast and ovarian cancer in Lithuania.

Key words: BRCA, breast and ovarian cancer, sceening

Corresponding author: Danielius Serapinas, Lithuanian University of Health Sciences, Medical Academy, Department of Genetics and molecular medicine Eivenių 2, Kaunas LT50009, Lithuania, Phone: +37037326771, fax: +37037326953, E-mail:dserapinas@gmail.com

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Published in „GENETIKA“ Vol. 49, No.1 (2017), pp. 51 -61

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701051I
Original scientific paper

DNA AND BIOCHEMICAL ANALYSIS OF A POTENTIAL OPAQUE2 MAIZE POPULATION

Dragana ignjatovic-micic¹, Ana nikolic¹, Aleksandra nikolic²,

Marija kostadinovic¹

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²Institute of Molecular Genetics and Genetic Engeneering, University of Belgrade, Serbia

Abstract

ignjatovic-micic D., A. nikolic, A. nikolic, M. kostadinovic (2017): DNA and biochemical analysis of a potential opaque2 maize population.-Genetika, Vol 49, No.1, 51-61.

Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples – with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.

Key words: maize, opaque2, sequencing, SSR, tryptophan, zeins

Corresponding author: Marija Kostadinovic, Maize Research Institute Zemun Polje, S. Bajica 1, 11185 Belgrade, Serbia, Tel: +381113756704, Fax: +381113756707, E-mail: kmarija@mrizp.rs

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 63 -76

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701063Y
Original scientific paper

APPLICATION OF COMPUTATIONAL ALGORITHMS TO ASSESS THE FUNCTIONALITY OF NON-SYNONYMOUS SUBSTITUTIONS IN MHC DRB GENE OF NIGERIAN GOATS

Abdulmojeed yakubu^1,2,3, Adebowale E. salako², Marcos de donato³^,4

and Ikhide G. imumorin³

¹Department of Animal Science, Nasarawa State University, Keffi, Shabu-Lafia Campus, Lafia, 950101, Nigeria

²Animal Breeding and Genetics Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Nigeria

³Animal Genetics and Genomics Laboratory, International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853. USA

⁴Laboratorio Genetica Molecular, Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas, Universidad de Oriente, Cumana, Venezuela.

Abstract

yakubu A., A. E. salako, M. de donato, I. G. imumorin (2017): Application of computational algorithms to assess the functionality of non-synonymous substitutions in MHC DRB gene of Nigerian goats.- Genetika, Vol 49, No. 1, 63 -76.

The Major Histocompatibility Complex (MHC) contains highly variable multi-gene families, which play a key role in the adaptive immune response within vertebrates. Among the Capra MHC class II genes, the expressed DRB locus is highly polymorphic, particularly in exon 2, which encodes the antigen-binding site. Models of variable non-synonymous/synonymous rate ratios among sites may provide important insights into functional constraints at different amino acid sites and may be used to detect sites under positive selection. Many non-synonymous single nucleotide polymorphisms (nsSNPs) at the DRB locus in goats are suspected to impact protein function. This study, therefore, aimed at comparing the efficiency of six computational approaches to predict the likelihood of a particular non-synonymous (amino acid change) coding SNP to cause a functional impact on the protein. This involved the use of PANTHER, SNAP, SIFT, PolyPhen-2, PROVEAN and nsSNPAnalyzer bioinformatics analytical tools in detecting harmful and beneficial effects at H57G, Y89R, V104D and Y112I substitutions in the peptide binding region of the DRB gene of Nigerian goats. The results from PANTHER analysis revealed that H57G, Y89R and Y112I substitutions (P_deleterious= 0.113, 0.204 and 0.472, respectively) were beneficial; while that of V104D was deleterious (P_deleterious= 0.756), an indication that it was non-neutral. As regards the SNAP approach, H57G and Y89R substitutions were returned neutral with expected accuracy of 53 and 69%, respectively while V104D and Y112I substitutions were harmful. H57G and Y89R substitutions were also found harmless in the SIFT analysis. However, only H57G (PROVEAN) and V104D (nsSNPAnalyzer) amino acid substitutions were found to be beneficial. Interestingly, the predicted 3D structures of both native and mutant DRB protein appeared similar as validated by Ramachandran plots. The consensus reached by PANTHER, SNAP, SIFT and PolyPhen-2 approaches on the neutrality especially of H57G (PROVEAN inclusive) and Y89R amino acid substitutions may be used in search of disease resistant genotypes at the DRB locus of Nigerian goats.

Key words: bioinformatics tools, DRB gene, Goats, Nigeria, Non-synonymous substitutions

Corresponding author: Abdulmojeed Yakubu, E-mail: abdulmojyak@gmail.com; Ikhide G. Imumorin, E-mail: igi2@cornell.edu

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Published in „GENETIKA“ Vol. 49, No.1 (2017), pp.77 -86

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701077S
Original scientific paper

GENETIC CHARACTERIZATION AND MOLECULAR MAPPING OF NOVEL CHLOROPHYLL DEFICIENCY GENE IN AIR-CURED TOBACCO (Nicotiana tabacum L.)

Zeinalabedin shahadati-moghaddam¹^,
2, Danial kahrizi³, Elham kazemi^*4

¹Tirtash Research and Education Center, Behshahr, Mazandaran, Iran

²Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Mazandaran, Iran

³Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

⁴Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

shahadati-moghaddamZ., D. kahrizi, E. kazemi (2017): Genetic characterization and molecular mapping of novel chlorophyll deficiency gene in air-cured tobacco (Nicotiana tabacum L.). - Genetika, Vol 49, No.1, 77 -86.

This study was performed to genetical and morphological investigation of a novel chlorophyll deficiency gene in tobacco leaf. One low chlorophyll content (LCC) variety (Urumieh 2) and high chlorophyll content (HCC) variety (Burley Ree 103) from the Burley type was crossed and the F₂ generation was grown on the field. One hundred plants were selected, contained low and high chlorophyll content. These plants were sampled and DNA was extracted. Sixty RAPD primers were tested on parents, LCC and HCC Bulks based on Bulk Segregant Analysis (BSA). Chi-square test confirmed the monogenic segregation. Regresion analysis showed that there was strong relationship between greenness degree and chlorophyll contents. Four primers (OPE17, OPC09, OPB08 and OPR02) showed polymorphism and after the test on 97 samples from the F₂ generation two markers were selected (OPB08-1050 and OPC09-1900). That showed 15.9 and 10.8 CM distance from chlorophyll locus respectively.

Keywords: BSA, chlorophyll, RAPD, tobacco

Corresponding author: Elham Kazemi, Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Tel: +98 918 3322235, Fax: +98 383 3832 10 83, email: ekazemi2002@yahoo.com

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Published in „GENETIKA“ Vol. 49, No.1 (2017), pp.87-94

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701077S
Original scientific paper

SECONDARY ASSOCIATIONS IN ‘HIMALAYAN PINK’ (Dianthus angulatus Royle

ex Benth., Caryophyllaceae) FROM COLD DESERTS OF LAHAUL-SPITI

Puneet kumar¹, Vijay Kumar singhal² and Sunil Kumar srivastava¹

¹Botanical Survey of India, Northern Regional Centre, Dehradun - 248 195, Uttarakhand, India

²Department of Botany, Punjabi University, Patiala, Punjab, India

Abstract

Kumar P., V. K. Singhal and S. K. Srivastava (2017): Secondary associations in ‘Himalayan Pink’ (Dianthus angulatus Royle ex Benth., Caryophyllaceae) from cold deserts of LahauL-Spiti.- Genetika, Vol 49, No.1, 87- 94.

In current investigation we studied the male meiosis and details of secondary chromosomal associations or pairing recorded for the first time in Dianthus angulatus Royle ex Benth., from the cold deserts of Lahaul-Spiti (Himachal Pradesh, India). All the presently studied individuals of the species existed at 2x level (x = 15). The present chromosome count of n = 15 in the species is in conformity with the previous counts from India and outside of India. Secondary associations are defined as the affinity of bivalents to be positioned in pairs having gentle connections. The secondary chromosomal associations in the species existed among bivalents/chromosomes were observed in the meiocytes at metaphase-I and continued till the metaphase-II. The bivalents positioned side by side and end to end to form secondary pairing. The difference in the number of bivalents/chromosomes involved in the secondary associations has also been witnessed. A secondary association between bivalents is considered to be of immense importance as it is being taken as a gauge of ploidy in plants. The incidence of such secondary associations of bivalents/chromosomes in D. angulatus which existed at 2x level indicated the secondary polyploid nature of the species.

Keywords: bivalents; chromosomes; Dianthus angulatus; Lahaul-Spiti, pollen mother cells

Corresponding author: Puneet Kumar, Botanical Survey of India, Northern Regional Centre, Dehradun - 248 195, Uttarakhand, India, E-mail: puneetbotcyto@gmail.com

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Published in „GENETIKA“ Vol. 49, No.1 (2017), pp. 95-104

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701095J
Original scientific paper

COMPARISON OF ELECTROPHORETIC PROTEIN PROFILES OF Brassica rapa

SUB-SPECIES BROWN SARSON THROUGH SDS-PAGE METHOD

Sohail ahmad jan¹^,
2, Zabta khan shinwari¹, Malik ashiq rabbani², Haris khurshid^{1, 2}, Muhammad ishaq ibrahim², Muhammad ADIL¹,

Muhammad ilyas²

¹Department of Biotechnology, Quaid-i- Azam University, Islamabad, Pakistan

²Plant Genetic Resources Institute, National Agricultural Research Centre, Islamabad, Pakistan

Abstract

jan ahmad S., Z. khan shinwari, M. ashiq rabbani, H. khurshid, M. ishaq ibrahim, M. Adil, M. ilyas (2017): Comparison of electrophoretic protein profiles of Brassica rapa sub-species brown sarson through SDS-PAGE method.- Genetika, Vol 49, No. 1, 95- 104.

Estimation of protein based variability among different Brassica sub-species is important for crop improvement. In present study total seed protein based variation among brown sub-specie of B. rapa was studied. Twenty different brown types’ genotypes were analysed through Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) method. The small, medium and large sizes proteins were noted. A total of 12 bands were obtained in which 10 (83.33%) are highly polymorphic while the rest two (15.38%) are monomorphic. The protein size base polymorphism was divided into different groups on the basis of molecular weight that ranges from ~10 kDa to ~180 kDa. The data of variable proteins were analysed through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method, which clustered all genotypes into four main cluster groups. The cluster I and II, III and IV consisted 3, 3, 6 and 10 genotypes respectively. The similarity coefficient values (40 to 96%) were calculated among different genotypes. The maximum similarity coefficient (96%) was recorded among genotypes Br-607, Br-560, and between Br-589 and Br-607 respectively. Our study showed maximum protein based diversity among brown sub-species of B. rapa which may serve as model to search protein based variation in other important plants sub-species.

Key words: Brassica rapa, brown sarson, phylogenetic relationship, SDS-PAGE

Corresponding author: Sohail ahmad jan, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan, Phone: +92-345-9118133, e-mail: sjan.parc@gmail.com, sohailahmadjan3@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 105 - 115

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701105C
Original scientific paper

HORSE CHESTNUT POLLEN QUALITY

Dušica ĆALIĆ, Ljiljana RADOJEVIĆ

Department of Plant Physiology, Institute for Biological Research “Siniša Stanković“,

University of Belgrade, Belgrade, Serbia

Abstract

Ćalić D. and Lj. Radojević (2017): Horse chestnut pollen quality.- Genetika, Vol 49, No. 1, 105-115.

Pollen quality of horse chestnut, expressed as pollen productivity, viability and germination was studied. Anthers of horse chestnut genotypes had pollen production from 3.66 to 5.06 x 10³pollengrains per anther, depending of genotype. Also, pollen of horse chestnut Ah1-Ah4 genotypes showed different viability (from 56 to 68%), after staining with fluorescein diacetate. Pollen germination of Ah1-Ah4 genotypes varied from 50-66% on basic medium. Inclusion of polyethylene glycol-PEG from 10%, 15% and 20% v/w increased pollen germination. The best results were achieved on medium with the largest PEG concentration. On these medium 76-91% pollen grains were germinated, depending of genotype. The best pollen quality, for all tested parameters, had genotype Ah2. Knowledge about morphology, production, viability, in vitro germination, tube growth as well as pollen: ovule ratio can be of great importance for future pollen biology studies.

Key words: Aesculus, pollen morphology, pollen:ovule ratio, pollen tube growth, pollen viability

Corresponding author: Dušica Ćalić, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković“, University of Belgrade, Belgrade, Serbia, Tel.: +381-11-2078-366; fax: +381-11-2761 433, E-mail address: calic@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 117- 126

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1701117N

Original scientific paper

HERITABILITY AND COMBINING ABILITY OF VEGETATIVE GROWTH AND PHENOLOGICAL DEVELOPMENT OF DIALLEL CROSSES OF RAPESEED

Hafsa naheed¹, Raziuddin¹, Sana abid¹, Quahir sohail², Ghulam hassan¹,

Muhammad arif³

¹Department of Plant Breeding and Genetics, University of Agriculture Peshawar, Pakistan

² Winter Wheat Breeding Program, ICARDA, Ankara, Turkey

³Department of Agronomy, The University of Agriculture Peshawar, Pakistan

Abstract

naheed H., Raziuddin, S. abid, Q. sohail, G. hassan, M. arif (2017): Heritability and combining ability of vegetative growth and phenological development of diallel crosses of rapeseed.- Genetika, Vol 49, No.1,125 - 134.

To estimate combining ability and heritability of F₂populations of 4 x 4 full diallel crosses and parents, an experiment was carried out at The University of Agriculture, during 2012-2013. Four parental lines and F₂populations of six direct and six reciprocal crosses were planted in the experiment using RCB design. Data were recorded on phenological and vegetative growth traits: Days to flowering, plant height, main stem length, main raceme length, primary branches, and days to maturity. Analysis of variance revealed significant variation among genotypes for all the parameters studied. The results of combining ability analysis showed that general combining ability (GCA) was highly significant for primary branches plant^-1, significant for plant height and days to physiological maturity and non-significant for the remaining traits. Specific combining ability (SCA) and reciprocal effects (RE) were significant for plant height, days to flowering, main raceme length and days to physiological maturity. Genotype AUP-401 was best general combiner for main raceme length, primary branches plant^-1 and days to physiological maturity. Among the crosses, AUP-404 x AUP-402 was best specific combiner for plant height, days to flowering and main stem length. Broad sense heritability was high (>70%) for plant height, main stem length and primary branches. Moderate heritability was observed for main raceme length, days to 50% flowering and days to physiological maturity. The variance components of SCA were greater than respective GCA components of all the characters signifying the presence of non-additive genetic effects in transfer of these traits and selection in the later generations should be practiced for improvement of these traits.

Keywords: combining ability, heritability, phenological development, rapeseed, vegetative growth

Corresponding author: Hafsa naheed, Department of Plant Breeding and Genetics, The University of Agriculture Peshawar, Pakistan. Phone: 091-5890447, e-mail: hafsaa.naheed@gmail.com

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Published in „GENETIKA“ Vol. 49, No.1 (2017), pp. 127 -138

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701127M
Original scientific paper

DETERMINATION OF S-GENOTYPE IN APPLE AND SWEET CHERRY CULTIVARS RELEASED AT FRUIT RESEARCH INSTITUTE, ČAČAK

Slađana MARIĆ^1*, Sanja RADIČEVIĆ¹, Milan LUKIĆ¹, Radosav CEROVIĆ²,

Svetlana A. PAUNOVIĆ¹

¹Fruit Research Institute, Čačak, Republic of Serbia

²Innovation Centre at Faculty of Technology and Metallurgy, University of Belgrade, Belgrade,

Republic of Serbia

Abstract

Marić S., S. Radičević, M. Lukić, R. Cerović, S. A. Paunović (2017): Determination of S-genotype in apple and sweet cherry cultivars released at Fruit Research Institute, Čačak.- Genetika, Vol. 49, No.1, 127-138.

Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple (‘Čačanska Pozna’ and ‘Čadel’) and two sweet cherry (‘Asenova Rana’ and ‘Čarna’) cultivars developed at Fruit Research Institute, Čačak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars ‘Čačanska Pozna’ (S₇S₉) and ‘Čadel’ (S₂S₇) were consistent with parental combinations ‘Starking Delicious’ (S₉S₂₈) × ‘Jonathan’ (S₇S₉) and ‘Golden Delicious’ (S₂S₃) × ‘Jonathan’ (S₇S₉), respectively. For sweet cherry cultivars ‘Asenova Rana’ [‘Drogans Gelbe’ (S₁S₅) × ‘Majova Rana’ (S₁S₄)] and ‘Čarna’ [‘Majova Rana’ (S₁S₄) × ‘Bigarreau de Schrecken’ (S₁S₃)], S₃S₉and S₁S₄ were identified respectively, suggesting that the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits.

Key words: Malus × domestica, Prunus avium, planned hybridisation, self-incompatibility, PCR amplification

Corresponding author: Slađana Marić, Fruit Research Institute, Čačak, Kralja Petra I/9, 32000 Čačak, Republic of Serbia, Phone: +381 32 221 375, Fax: +381 32 221 391, E-mail: nidzovicsladja@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 1 (2017), pp. 139-149

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701139G
Original scientific paper

SOURCES OF RESISTANCE TO RACES R0 AND R1 OF Pseudomonas syringae pv. tomato – AGENT OF BACTERIAL SPECK ON TOMATO

Daniela ganeva¹ and Nevena bogatzevska²

¹”Maritsa”Vegetable Crops Research Institute, Plovdiv, Bulgaria

²Institute of Soil Science, Agrotechnologies and Plant Protection “N. Pushkarov”, Sofia, Bulgaria

Abstract

ganeva D. and N. bogatzevska (2017): Sources of resistance to races R0 and R1 of Pseudomonas syringae pv. tomato – agent of bacterial speck on tomato. - Genetika, Vol 49, No.1, 139 -149.

Tomato breeding lines with fruit colour different from the traditional red colour were studied in order to search for sources of resistance to races R0 and R1 of Pseudomonas syringae pv. tomato. As a result of selection of healthy plants with hypersensitive response (HR), the resistance was stabilized and perspective lines gene-carriers of resistance to bacterial speck were chosen. Lines L1078 and L1083 with brown-red (black) coloured fruits and line L1130 with purple-red fruits possess a complex resistance to races R0 and R1. It was established that two of the lines with rose-coloured tomato fruits (L1088 and L584) were resistant to race 1 of P. syringae pv. tomato. These lines possessed valuable economic and morphological characters and they could be used in combinative and heterosis breeding for development of resistance to bacterial speck varieties.

Keywords: quality, Pseudomonas syringae pv. tomato races 0,1, resistance, Solanum lycopersicum L.

Corresponding author: Daniela Ganeva, ”Maritsa” Vegetable Crops Research Institute, 32, Brezovsko shosse Str., 4003 Plovdiv, Bulgaria, dganeva@abv.bg

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Published in „GENETIKA“ Vol. 49, No.1(2017), pp. 151- 160

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701151V

Original scientific paper

RELATIONSHIP BETWEEN THE GENETIC hemoglobin POLYMORPHISM, morphometry and fertility of Pramenka sheep breed FROM central bosnia

Božo S. važić¹. Biljana S. rogić¹. Milanka S. drinić¹. Novo M. pržulj¹

¹University of Banja Luka, Faculty of Agriculture, Banja Luka, Bosnia and Herzegovina

Abstract

važić B., B. rogić, M. drinić, N. pržulj (2017): Relationship between the genetic hemoglobin polymorphism, morphometry and fertility of Pramenka sheep breed from central Bosnia.- Genetika, Vol 49, No.1, 151-160.

Pramenka sheep belong to the group of primitive sheep breeds with a triple-purpose production: milk, meat and wool. None of these production directions is emphasized. Sheep of this breed are the reflection of the environment in which they are located. The characteristics of these sheep are highlighted depth measures of external appearance with modest width measures. Another weaker feature of Pramenka sheep is poor fertility. Despite the mentioned disadvantages, Pramenka sheep is the most grown sheep in Central Bosnia and sheep production is based on it. Methodical selection to improve the characteristics of Pramenka sheep has not applied. However, sheep breeders tend to improve the qualitative and quantitative characteristics of sheep, and that means more intensive work on the selection. In this sense, genetic markers are used to carry out the selection lately. Three types of hemoglobin, HbAA, HbAB and HbBB of Pramenka sheep population (at 189 male and female animals) in Central Bosnia were segregated by the method of electrophoresis. The following genotype frequencies are determined: HbAA 0.11; HbAB 0.41; and HbBB 0.48. Allele frequencies, HbA and HbB for Pramenka sheep breed (estimate based on genotype frequencies) were 0.315 and 0.685. It was found that the population of sheep was in the equilibrium of the frequency of hemoglobin genotypes. Sheep with HbAA genotype had lower morphometric measures in relation to the other two genotypes. The statistically significant difference between sheep with HbAB genotype and HbAA genotype was recorded only for the shin perimeter. The ewes that lambed one lamb have the following frequency of HbAA, HbAB and HbBB genotypes: 0.13; 0.40 and 0.47, and the ewes with twins: 0.02; 0.40 and 0.58. Fertility, as important quantitative characteristic of sheep, was more emphasized in genotype HbBB, than in HbAA genotype. This was confirmed by the statistical analysis.

Key words: fertility, hemoglobin, morphometric characteristics, Pramenka sheep, selection

Corresponding author: Božo Važić, University of Banja Luka, Faculty of Agriculture, Bulevar vojvode Petra Bojivića 1A, 78000 Banja Luka, Bosnia and Herzegovina, phone: 0038751330965, fax: 0038751312390, e-mail: vazicb@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 161- 172

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1701161T

Original scientific paper

ASSESSMENT OF SERBIAN PEPPER VARIETIES GROWN

IN CONDITIONS OF SOUTH BULGARIA

Velichka todorova¹, Ivo DJINOVIC²

¹Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria

²Superior Seed Company, Velika Plana, Serbia

Abstract

todorova V. and I. Djinovic (2017): Assessment of Serbian pepper varieties grown in conditions of south Bulgaria.- Genetika, Vol 49, No.1, 161-172.

The comparative estimation was carried out with eight Serbian pepper varieties (Capsicum annuum L.) during the period 2010-2011 in field conditions at Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria. The varieties were evaluated by some important morphological traits of the plant and fruit, total and marketable yield. The assessment by plant and stem height showed proved differences between them. Romansa and Ekstaza formed significantly longer fruits than other varieties while Amanda was with the lowest values by this trait but with the highest ones by the diameter at the base and flesh thickness. Romansa, Ekstaza and Delfina were most productive before maturity stage with 46.04 t ha^-1, 45.31 t ha^-1 and 45.13 t ha^-1, respectively. The varieties which were evaluated by yield of the fruit at maturity stage showed non proved differences by total yield and their values were from 21.34 t ha^-1 for Delfina to 24.41 t ha^-1 for Slonovo uvo.

Key words: Capsicum annuum L., plant, fruit, yield

Corresponding author: Velichka Todorova, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse 32, 4003 Plovdiv, Bulgaria, Tel.: +35932952296, Fax: +35932960177, e-mail: todorova_vili@abv.bg

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 173-181

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701173S
Original scientific paper

SCREENING OF CAMELINA (Camelina sativa L.) DOUBLED HAPLOID LINES FOR FREEZING TOLERANCE IN THE SEEDLING STAGE

Jahad SOORNI^1*, Seyed Kamal KAZEMITABAR¹, Danial KAHRIZI², Ali DEHESTANI³, Nadali BAGHERI¹

¹Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Mazandaran, Iran

²Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

³Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Soorni J., S. K. Kazemitabar, D. Kahrizi, A. Dehestani, N. Bagheri (2017): Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage. - Genetika, Vol 49, No.1, 173-181.

Freezing stress is an important abiotic stress limiting the yield and the spatial distribution of many important crops. This study was undertaken to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.) along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and Cascade) as experimental control under freezing stress conditions (-14 °C for 6 h) to identify lines with high or low level of tolerance to freezing for further studies. First, a protocol was developed for large scale screening of camelina germplasm under freezing stress. For this purpose, an experiment with different freezing temperatures (-5, -10, -15 and -20 °C) was conducted to find an appropriate temperature to discriminate best between genotypes (i.e. the LT₅₀ temperature). The LT₅₀ values for camelina lines varied between -10.2 and -17.1 °C with an average of -13.94 °C. Therefore, we selected the -14 °C exposure for 6 h as an appropriate temperature for screening camelina lines. The freezing-tolerant and freezing-sensitive lines were determined using biplot analysis of measured parameters (LT₅₀, survival percentage, relative conductivity and scoring). Among 136 DH lines, some lines had high levels of freezing tolerance (58, 62 and 101) and some of them were sensitive to freezing (8, 16, 32, 91 and 107). The selected lines with high or low level of freezing tolerance in a preliminary screening test are useful for further experiments and cultivation in the area with freezing stress in the seedling stage.

Keywords: biplot, canola, LT₅₀, principal component, relative conductivity

Corresponding author: Jahad Soorni, Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Mazandaran, Iran. E-mail: d.soorni@gmail.com, Phone: +98 9120234467,Fax: +98-11-33687715

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp.183-191

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701183K
Original scientific paper

GENOTYPE EFFECTS ON PHOSPHORUS, POTASSIUM, CALCIUM

AND MAGNESIUM STATUS IN MAIZE

Vlado kovačević¹, Ivan brkić², Manda antunović¹,

Dario iljkić¹, Ivana varga¹

¹ University J. J. Strossmayer in Osijek, Faculty of Agriculture, 31000 Osijek, Croatia

²Agricultural Institute Osijek, 31000 Osijek, Croatia

Abstract

kovačević V., I. brkić, M. antunović, D. iljkić, I. varga (2017): Genotype effects on phosphorus, potassium, calcium and magnesium status in maize. - Genetika, Vol 49 No. 1, 183-191.

Nine maize lines, commonly used as female parents of maize hybrid (B1=♀2-48; B2=♀1767/99; B3=♀ 87-24; B4=♀135-88, B5=♀84-28; B6=♀84-44; B7=♀438-95; B8=♀30-8; B9=♀B-73) were grown under field conditions on Podgorac acid soil in Osijek-Baranya County for two growing seasons (2006 and 2007). The ear-leaves at flowering and grain at maturity were taken from each basic plot (14 m²) for chemical analysis with inductively coupled plasma atomic emission spectroscopy (ICP-OES). Average concentrations (2-year means: g kg^-1indry matter) were as follows: 3.21 and 3.00 (P), 20.8 and 3.45 (K), 6.60 and 0.05 (Ca), 2.44 and 1.04 (Mg) for leaves and grain, respectively. Differences among genotypes were from 2.69 to 3.95 and from 2.70 to 3.57 (P), from 18.0 to 23.3 and from 3.03 to 3.71 (K), from 5.45 to 8.02 and from 0.04 to 0.07 (Ca), from 1.35 to 3.09 and from 0.84 to 1.36 (Mg), for leaves and grain, respectively. Specifies of leaf composition of individual genotypes were as follows: B1 (the highest Ca and Mg), B2 (the highest P), B4 (the lowest Ca, Mg and P), B6 (the highest K) and B7 (the lowest K). Grain composition was mainly in accordance with specifies of leaf composition. Very high correlation in maize mineral composition under identical environmental conditions for nine genotypes between two years (0.97***, 0.97*** and 0.91*** for K, Ca and Mg, respectively) are indication of high hereditary effects, while P was more under environmental impact (r = 0.43). Significant correlations were found between grain-P and grain-K (0.55*), grain-Ca (0.49*) and grain-Mg (0.86***), grain-Ca and grain-Mg (0.54*). However, regarding mineral composition of leaves, only leaf-Ca and leaf-P had significant correlation (-0.46*).

Key words: maize lines, calcium, magnesium, phosphorus, potassium, leaves and grain

Corresponding author: Iljkić Dario, Faculty of Agriculture, Vladimira Preloga 1, 31000 Osijek, Croatia; Phone: ++385 31 554 901, Fax: ++385 31 554 853, E-mail: diljkic@pfos.hr

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 193-202

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701193P

Original scientific paper

SENSORY, CHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF Cucurbita maxima AND Cucurbita moschata GENOTYPES FROM DIFFERENT GEOGRAPHICAL ORIGINS

Galina pevicharova and Nikolay velkov

Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria

Abstract

pevicharova G. and N. velkov (2017): Sensory, chemical and morphological characterization of Cucurbita maxima and Cucurbita moschata genotypes from different geographical origins.- Genetika vol 49, no1, 193-202.

Cucurbita spp. is one of the most important vegetable crops in the world. They are characterized by great polymorphism according plants and fruits traits. The fruits are consumed in different ways as boiled, baked, dried or processed in puree and juice. The aim of the study was to assess variation of fruit sensory, chemical and morphological characteristics of Cucurbita genotypes in order to find appropriate parental components for the future high quality breeding programme. During 2-year period nine winter squash (Cucurbita maxima Duch.) and three pumpkin (Cucurbita moschata Duch.) genotypes originating from different geographical regions were tested on their morphological characters, basic chemical components and sensory profile. Cluster analysis and Principle component analysis were applied in order to identify similarities of different genotypes. According to the fruit characteristics and plant habitus significant differences were recorded. Considerable variation in the content of dry matter, ascorbic acid, total sugars, total pigments, beta-carotene and sensory assessment of the boiled fruits was established. Accession Moskatna carotina had the best flavour and chemical composition. The studied Cucurbita genotypes are a good basis for performing a breeding program to improve the sensory quality of fruits and increase basic chemical components especially those with antioxidant effect.

Key words: ascorbic acid beta-carotene, flavour, squash, total sugars

Corresponding author: Nikolay Velkov, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse Str. 32, 4003 Plovdiv, Bulgaria, Tel.:+35932952296, Fax:+35932960177, e-mail: velkov_n@abv.bg

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 203-215

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701203T
Original scientific paper

MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF Phytophthora SPECIES ISOLATED FROM THE RHIZOSPHERE OF DECLINING OAK TREES IN KROTOSZYN PLATEAU

Miłosz tkaczyk¹, Ivan milenković^2,3, Justyna A. nowakowska¹, Małgorzata borys¹,

Tomasz kałuski⁴, Magdalena gawlak⁴, Michał czyż⁴, Tomasz oszako¹

¹Forest Research Institute-IBL, Raszyn, Poland

²Phytophthora Research Centre, Mendel University, Brno, Czech Republic

³Institute of Forestry, Belgrade, Serbia

⁴Institute of Plant Protection-National Research Institute, Poznań, Poland

Abstract

tkaczyk M., I. milenković, J. A. nowakowska, M. borys, T. kałuski, M. gawlak, M. czyż, T. oszako (2017): Morphological and molecular identification of Phytophthora species isolated from the rhizosphere of declining oak trees in Krotoszyn plateau.- Genetika vol49, no1, 203- 215.

The following paper presents the results on the determination of the diversity of species from the Phytophthora genus occurring in the declining oak stands in Krotoszyn Plateau in Poland. From the 50s of the last century, significant deterioration of oak health was observed in these stands, and Phytophthora species were suggested as one of the factors of the decline. In order to determine the presence of pathogenic organisms from the Phytophthora genus in these stands, 180 rhizosphere soil samples from three forest districts throughout the Krotoszyn Plateau were collected and subjected to the isolation method. Phytophthora species were consistently isolated from all the sampled stands, and 194 isolates from 111 positive samples were obtained. However, 150 (77%) and 44 (23%) isolates originated from the samples taken under the symptomatic and asymptomatic trees, respectively. All the obtained isolates were morphologically classified using the light and scanning electron microscopy and divided into morphological groups. Genomic DNA was isolated from selected isolates representing each group, ITS regions were amplified and sequence analyses were performed. In total, four different Phytophthora species were detected, including P. cactorum, P. plurivora, P. quercina and P. europaea. The most often isolated species were P. cactorum and P. plurivora. This is the first report of P. europaea in oak stands in Poland.

Key words: morphology, PCR, Phytophthora, Quercus robur, Sequencing, SEM

Corresponding author: Milosz Tkaczyk; Forest Research Institute; ul. Braci Lesnej 3; Sekocin Stary; 05-090 Raszyn; Poland; Phone:+48227153823; e-mail: m.tkaczyk @ibles.waw.pl

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 217-234

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR1701217K

Original scientific paper

POLLEN EFFECT (XENIA) FOR EVALUATING BREEDING MATERIALS IN MAIZE

Fatih KAHRIMAN¹, Mehmet ŞERMENT¹, Mizgin HAŞLAK¹, Manjit S. KANG²

¹Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Turkey

²Kansas State University, Department of Plant Pathology, Manhattan, KS, 66506-5502, USA

Abstract

Kahriman F., M. Şerment, M. Haşlak, M. S. Kang (2017): Pollen effect (xenia) for evaluating breeding materials in maize.- Genetika vol49, no1, 217-234.

Pollen effect (xenia) plays an important role in modifying biochemical constituents of maize (Zea mays L.) kernels. The objectives of this study were to i) evaluate the effect of filial generations on certain genetic estimations; ii) compare general (GCA) and specific combining ability (SCA) effects obtained from Griffing’s diallel analyses between parental (F₀) and F₁ generations; iii) determine the relationships between pollen effect and genetic estimations; and iv) examine the possible utility of pollen effect for improving kernel-quality traits (protein and oil contents). We conducted two experiments (F₀ in 2011 and F₁ in 2013) and examined kernel protein and oil contents. Individual pollen effects (IPE) and specific individual pollen effects (SIPE) were computed. The results showed that entries (E) and filial generations (G) and E × G interaction variances were significant for both protein and oil contents, whereas changes in genetic estimates between generations were highly variable. The signs and magnitudes of GCA effects were similar and highly correlated (r > 0.80) between F₀ and F₁ generations for all four diallel methods. In addition, GCA effects were highly correlated with IPE estimates for all four diallel methods. Specific combining ability (SCA) estimates between F₀ and F₁ generations were moderately correlated (r = 0.50) in Method IV for oil and highly negatively correlated (r = -1.00) in Method III for protein content. Heterosis analyses showed that hybrids could not be evaluated on the basis of the F₀ generation to predict their F₁ performances. Individual pollen effects between generations showed higher correlation for protein content (r = 1.00) than for oil content (r = 0.40). Specific individual pollen effects of parents were also slightly higher for protein content (r = 0.74) than for oil content (r = 0.62). We concluded that the direct or indirect utilization of pollen effect (xenia) was possible for parental evaluation but not suitable for hybrid evaluation for kernel oil and protein contents.

Key words: combining ability, diallel analysis, kernel quality, oil content, protein content, Zea mays

Corresponding author: Fatih Kahriman, Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Turkey, Tel: +90 2862180018/1354,e-mail: fkahriman@hotmail.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 235-246

Serbia

UDC 575.633.11
https://doi.org/10.2298/GENSR1701235S

Original scientific paper

THE LACK OF CORRELATION BETWEEN TP53 MUTATIONS AND GASTRIC CANCER: A REPORT FROM A PROVINCE OF IRAN

Javad saffari-chaleshtori¹, Mohammad-Amin tabatabaiefar²,

Payam ghasemi-dehkordi³, Effat farokhi³, Mohammad-Taghi moradi³,

Morteza hashemzadeh-chaleshtori³

¹ Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

² Department of Medical Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

³ Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

saffari-chaleshtori J., M.-A. tabatabaiefar, P. ghasemi-dehkordi, E. farokhi, M.-T. moradi, M. hashemzadeh-chaleshtori (2017): The lack of correlation between TP53 mutations and gastric cancer: a report from a province of Iran.- Genetika vol49, no1, 235-246.

Gastric cancer ranks second cause of cancer death worldwide after lung cancer. Its etiology is heterogeneous and genetic factors including protooncogenes and tumor suppressor genes always contribute to the progression of cancer. The TP53 tumor suppressor gene has a broad role in genomic stability and DNA repair. The aim of this study was to determine the TP53 gene mutations in gastric cancer specimens in Chaharmahal Va Bakhtiari province of Iran. In this descriptive-lab based study, we investigated the promoter and exons of TP53 gene mutations in 38 paraffin-embedded gastric cancer specimens. DNA was extracted following a standard phenol-chloroform protocol. The TP53 gene mutations were determined using PCR-SSCP & PCR-RFLP procedures. The present study revealed no TP53 gene mutation in the promoter and exons in the gastric cancer subjects studied. While TP53 gene mutations have been reported as the most frequent genetic alterations and are found in about 50% of the human malignancies, no mutation was detected in this study. This may be due to mutations in other related genes in the same pathway or epigenetic factors.

Key words: gastric cancer, Iran, PCR-SSCP, RFLP, TP53 gene

Corresponding author: Morteza Hashemzadeh-Chaleshtori, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran, Postal code: 8813833435, Tel: +98-38-33346692. Fax: +98-38-33330709. E-mail: molecularskums@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 247-258

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701247H
Original scientific paper

GENETIC VARIATION OF BULGARIAN AUTOCHTHONOUS SHEEP BREEDS USING MICROSATELLITE MARKERS

Deyana HRISTOVA¹, Stoycho METODIEV¹, Vassil NIKOLOV³, Dimitar VASSILEV²,

Elena TODOROVSKA²

¹Department of Genetics, Animal Breeding and Reproduction, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria

²AgroBioInstitute, 1164 Sofia, Bulgaria

³ Executive Agency of Animal Breeding and Reproduction, Sofia

Abstract

Hristova D., S. Metodiev, V. Nikolov, D. Vassilev, E. Todorovska (2017): Genetic variation of Bulgarian autochthonous sheep breeds using microsatellite markers.- Genetika, Vol 49, No1, 247- 258.

The aim of the study is focused on the design of a conservation sheep breeding programme for the studied autochthonous breeds based on SSR markers genetic variation analysis applied. Seven local Bulgarian autochthonous sheep breeds (Breznishka, Sofiiska, Copper-Red Shumenska, Karakachanska, Local Karnobatska, Blackhead Plevenska and Starozagorska) were differentiated using six microsatellite (SSR) markers, aiming to assess the genetic variation within and between breeds. Among the total of 96 identified alleles, eighteen population specific ones were detected across the all studied genotypes of 338 individuals, except in the Blackhead Plevenska sheep. All examined breeds indicated high level of genetic diversity, with an average of 0.792. The genetic differentiation between the examined sheep breeds was not significant and the values of genetic distances were relatively low. The analysis of molecular variances (AMOVA) showed low variation between the examined breeds (5.51%) in comparison to within population variation (94.49%). The greatest distance (0.643) was found between the populations Local Karnobatska and Starozagorska, while the smallest one (0.108), between the Copper-Red Shumenska and Karakachanska. The genetic distances calculated by Neighbour-Joining method, produced a phylogenetic tree which separates the investigated sheep breeds into two main clusters: one including Blackhead Plevenska Breznishka and Local Karnobatska, and the other one consisting of the four remaining breeds – Copper-Red Shumenska, Karakachanska, Sofiiska and Starozagorska sheep. The Factorial Correspondence Analysis (FCA) showed isolation of Local Karnobatska sheep and Starozagorska and an admixture of the other populations.

Key words: diversity, genetic distances, microsatellite (SSR) markers, Ovis aries

Corresponding author: Deyana Hristova, PhD, Department of Genetics, Animal Breeding and Reproduction, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria, tel: + 359 42 699 362, e-mail: dhrisova@uni-sz.bg.

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 259-272

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701259C

Original scientific paper

NORWAY SPRUCE (Picea abies Karst.) VARIABILITY IN PROGENY TESTS IN BOSNIA AND HERZEGOVINA

Branislav cvjetković¹, Monika konnert², Barbara fussi², Milan mataruga¹, Mirjana šijačić-nikolić³, Vanja daničić¹, Aleksandar lučić⁴

¹ University of Banja Luka, Faculty of Forestry, Bosnia and Herzegovina

² Bavarian offices for Forest Seeding and Planting, D-83317, Teisendorf, Germany

³ University of Belgrade, Faculty of Forestry, Belgrade, Serbia

⁴ Institute of Forestry, Belgrade, Belgrade, Serbia

Abstract

cvjetković B., M. konnert, B. fussi, M. mataruga, M. šijačić-nikolić, V. daničić, A. lučić (2017): Norway spruce (Picea abies Karst.) variability in progeny tests in Bosnia and Herzegovina.- Genetika vol 49, no1, 259- 272.

Norway spruce is one of the most important economic species is Bosnia and Herzegovina. It is the species at the edge of its natural range; nevertheless it attains significant growth and yield results. The species is often used for afforestation purposes. In the previous period, 4 progeny tests of Norway spruce were established in Bosnia and Herzegovina. The offspring from 6 natural populations: Han Pijesak 1, Han Pijesak 2, Foča, Potoci, Olovo and Kneževo were build-in the progeny tests. In 2016, the samples of Norway spruce from 2 progeny tests: Srebrenica and Drinić were collected. In total, 360 samples were collected. DNA isolation was done according to Dumoline et al. (1990). For assessment of genetic differences among populations, co-dominant nSSR microsatellite system had been used. The number of effective alleles ranged from 7.78 in the population Potoci up to 15 in the population Kneževo, the average number of alleles was 13. The observed heterozygosity ranged from 0.61 for the population Han Pijesak 2 up to 0.68 for population Kneževo. The average observed heterozygosity was 0.65. Fixation index was in the range from -0.073 in the population Potoci, to 0.030 for the population Han Pijesak 2. The average value of Wright fixation index is -0.007. The average fixation index indicates the existence of a very small number of homozygotes. Concerning the variability among populations it has been concluded that the total level of genetic differentiation among populations was very low (F_ST= 0.026). The result of Nei’s genetic distance shows that the populations Olovo and Potoci are separated from other populations. The results obtained by genetic markers, in addition with other, morphological and physiological markers will be the basis for the further investigation of Norway spruce adaptability and possibility for the transfer of genetic material in light of climate changes.

Key words: Norway spruce, nSSR markers, progeny tests, Bosnia and Herzegovina

Corresponding author: Branislav Cvjetković, Univeristy of Banja Luka, Faculty of Forestry, Stepe Stepanovića 75A, 78000, Banja Luka, Bosnia and Herzegovina. Phone: +387-51-460-550. E-mail: branislav.cvjetkovic@sf.unibl.org

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp 273-284

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701273A

Original scientific paper

CORRELATION AND PATH ANALYSIS FOR GRAIN YIELD AND YIELD COMPONENTS IN CHICKPEA (Cicer arietinum L.)

Hossein Astereki¹, Peyman Sharifi^*2, Masoumeh Pouresmael³

¹Research Instrutor-Research Center of Agriculture and Natural Resources of Lorestan, Iran

² Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Iran

³Member of Scientific-Plant and Seed Improvement Institute, Karaj, Iran

Abstract

Astereki H., P. Sharifi, M. Pouresmael (2017): Correlation and path analysis for grain yield and yield components in chickpea (Cicer arietinum L.).- Genetika, Vol 49, No. 1, 273 - 284.

Chickpea is the second most important pulse crop in the world after dry bean. The aim of this study was to estimate the correlation coefficients and path analysis between seed yield, morphological traits and yield components. Twenty five chickpea genotypes, including advanced lines and commercial varieties, were grown under dryland condition at Brojerd Agricultural Research Station (west of Iran) during two seasons (2012-2013 and 2013-2014). The field experiment was arranged in a randomized complete block design (RCBD) with four replications. Combined analysis of variance revealed that the studied genotypes differed significantly in seed yield, days to flowering, days to maturity, flowering period, canopy height, number of pods per plant, biological yield and harvest index. The correlation coefficients indicated that there were significant and positive correlations between seed yield and number of pods per plant and harvest index in two years. Path analysis indicated that days to flowering, days to maturity, canopy height and width, number of pods per plant and flowering period directly and indirectly affected seed yield. Therefore, this study suggested that chickpea improvement program could be based on these traits, especially number of pods per plant and canopy height, according to positive direct effect on seed yield over two years, as selection criteria.

Key words: breeding, chickpea, correlation, path analysis, seed yield

Corresponding author: Peyman Sharifi, Department of Agronomy and Plant Breeding, Faculty of Agriculture ,Islamic Azad University, Rasht, Ira, Email: Peyman.sharifi@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 285-295

UDC 575

https://doi.org/10.2298/GENSR1701285A
Original scientific paper

DETERMINATION OF SOMATIC AND SEX CHROMOSOMES OF THREE EGYPTIAN BIRDS SPECIES USING CYTOGENETIC ANALYSIS

a. h. abu-almaaty

Zoology Department, Faculty of Science, Port Said University, Egypt

Abstract

abu-almaaty a. h. (2017): Determination of somatic and sex chromosomes of three Egyptian birds species using cytogenetic analysis.- Genetika, Vol 49, No. 1, 285-295.

Study of karyotypes has been revealing important information on the taxonomic relationships and evolutionary patterns in various groups of birds. Karyotypes analysis and morphometric measurement of the chromosomes of three birds species of Geopelia cuneata (Columbiformes), Oriolus oriolus and Corvus ruficollis (Passeriformes) and their karyological have been studied. The diploid chromosome numbers of three species were, 2n=72, 2n=80 and 2n =80 respectively. The karyotypes of three species were different. The somatic and sex chromosomes and karyotypes of three species discussed and compared with other related species in the present study.

Key words: birds, cytogenetics, karyotype, macrochromosomes, microchromosomes, ZW sex chromosome.

Corresponding author: Ali Hussein Abu –Almaaty, Department of Zoology, Faculty of Science, Port Said University, Port Said, 42521, Egypt,Tel: 00201093041699, E-mail: ali_zoology_2010@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 297-311

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701297A
Original scientific paper

Morpho-physiological characterization of soybean genotypes under SUBtropical environment

M A Gaffar al-hadi¹, M Rafiqul islam¹, M Abdul karim¹, M Tofazzal islam²

¹Department of Agronomy, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh

²Department of Biotechnology, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh

Abstract

al-hadi M. A. G., M. R. islam, M. A. karim, M. T. islam (2017): Morpho-physiological characterization of soybean genotypes under subtropical environment.- Genetika, Vol 49, No.1, 297 - 311.

Soybean is a promising oilseed crop in rice-based cropping systems in South and Southeast Asia. In spite of immense scope of its expansion, the crop is not being popular to the farmers because of poor yield of the existing cultivars. Therefore, this study evaluated eighty-soybean genotypes of diverse growth habits with a view to searching genotype(s) of desirable morpho-physiological characters and high yield potential. Sixteen quantitative plant traits were evaluated to classify the genotypes into different groups using various multivariate methods. A wide range of variation was found in almost all qualitative plant traits. The study reveals that plants tend to become taller as the phenological cycle is longer. Seed yield was the product of the number of pods per plant, pod weight and seeds per pod. The first three components of principal component analysis explained 75% of the total variations of the soybean genotypes. Using Dendrogram from cluster analysis, the genotypes were grouped into six clusters. The maximum number of genotypes was concentrated in cluster 5 followed by clusters 4. The phenology, plant height, the number of pods and seed yield were the important discriminating variables in grouping the genotypes. The number of pods per plant displayed the principal role in explaining the maximum variance in the genotypes. The clustering pattern of the genotypes revealed that the genotypes under cluster 2 and cluster 6 were long statures, late maturing and produced higher seed yield. The genotype G00003 under cluster 2 is the best entry giving the highest seed yield. From cluster 6, the genotype G00209 could be the better choice for much better seed yield. The cluster 3 genotypes were comparatively early maturing and gave reasonable yield. It is concluded that the genotypes under clusters 2 and 6 and 3 can be important resources for developing a high yielding variety and sustainability of growing soybean in the subtropical conditions.

Key words: genotypes, morpho-physiology, multivariate analysis, soybean

Corresponding author: M Rafiqul Islam, Department of Agronomy, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, E-mail: rafiarib@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 1(2017), pp. 313-328

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1701313J

Original scientific paper

GENOTYPIC VARIATIONS FOR PHOTOASSIMILATES PARTITIONING TO THE GRAINS DURING EARLY DEVELOPMENT OF ENDOSPERM IN WHEAT: ASSOCIATION WITH GRAIN WEIGHT

Mehdi joudi

Meshkin–Shahr College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Joudi M. (2017): Genotypic variations for photoassimilates partitioning to the grains during early development of endosperm in wheat: association with grain weight.- Genetika, Vol 49, No.1, 313 -328.

There is little information on genotype variations for photoassimilates partitioning to the grains during early development of endosperm in wheat. Eighty–one wheat cultivars were examined in the Moghan region of Iran during 2010–2011 and 2013–2014 growing seasons. The amount and rate of photoassimilates partitioning to the grains were measured in the tested cultivars during anthesis–16 days after anthesis (DAA) and 16 DAA–maturity phases using time dependent changes in spike dry weight. There were substantial genetic variations in the amount and rate of partitioned photoassimilates to the grains during anthesis–16 DAA and 16 DAA–maturity phases. Part of these variations could be attributed to cultivars differences in anthesis time, spike dry weight at anthesis, and grain number per spike. Taking them into accounts, there were some cultivars but they differed in partitioned photoassimilates toward grains during anthesis–16 DAA and 16 DAA–maturity phases, further supporting the idea that breeding for photoassimilates partitioning during these phases was a possibility. The results yielded for 2013–2014 showed that there is close association between photoassimilates partitioning to the grain during anthesis–16 DAA and individual grain weight. The results suggest that in case wheat breeders could improve photoassimilates partitioning to the grains during anthesis–16 DAA, it would result in increased grain size and weight in the wheat.

Key words: grain growth stages, grain weight, photoassimilates partitioning, wheat

Corresponding author: Mehdi joudi, Meshkin–Shahr College of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran, Email: mehdijoudi@gmail.com; joudi@uma.ac.ir

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Published in „GENETIKA“ Vol. 49 No. 1(2017), pp. 329-344

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701329K
Original scientific paper

GENETIC DIVERSITY ANALYSIS IN ELITE LINES OF TOMATO (Solanum lycopersicum L.) FOR GROWTH, YIELD AND QUALITY PARAMETERS

Simranjit kaur, Salesh K. jindal, Major S. dhailwal, Neena chawla,

Om Prakash meena

Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004,

Punjab, India

Abstract

kaur S., S. K. jindal, M. S. dhailwal, N. chawla, O. Prakash meena (2017): Genetic diversity analysis in elite lines of tomato (Solanum lycopersicum L.) for growth, yield and quality parameters.-Genetika, Vol 49, No.1, 329 -344.

To increase productivity in tomato, it is necessary to develop superior varieties/hybrids. This is, in part, dependent on variability in the genetic material which affects agro-morphological and biochemical characters in crop breeding. A study was conducted with 51 tomato genotypes and the standard checks/reference cultivars Punjab Upma, Punjab Chuhhara and Punjab Ratta to determine character association, path coefficient analysis and genetic diversity to select genotypes and traits for breeding. There were differences among genotypes for all characters indicating a high degree of variability in the material. Overall, highly significant, positive, correlation coefficients, as well as high direct effects of fruit weight and marketable yield on total fruit yield, indicated these traits are reliable components for selecting high fruit yielding tomato genotypes. The D² statistics confirmed the highest inter-cluster distance between clusters VI and VIII (27638.44). Maximum similarity was observed in clusters IV and VI (191.02). This indicated existence of the possibility to improve genotypes through hybridization from any pair of clusters and subsequent selection can be made from segregant generations.

Key words: genetic diversity, genetic relationship, morphological traits, Solanum lycopersicum L.

Corresponding author: Salesh K. jindal, Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, Punjab, India, E-mail: saleshjindal@pau.edu

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Published in „GENETIKA“ Vol. 49, No.1(2017), pp. 345-354

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701345A
Original scientific paper

CHARACTERISATION OF B KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES AND TELOMERIC AND CENTROMERIC MOTIFS IN HEMATOPOIETIC STEM CELL TRANSPLANTATION DONORS IN VOJVODINA, SERBIA

Dušica ademović-sazdanić ¹, Svetlana vojvodić¹, S. POPOVIĆ²,

N. KONSTANTINIDIS³

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics,

Tissue Typing Laboratory, University of Novi Sad, Medical faculty Novi Sad

²Institute for Internal medicine, Department of hematology, Clinical center of Vojvodina, University of Novi Sad, Medical faculty Novi Sad

³Institute for Child and Youth Health Care of Vojvodina, Department for oncology

and hematology, University of Novi Sad, Medical faculty Novi Sad

Abstract

ademović-sazdanić D., S. vojvodić, S. Popović, N. Konstantinidis (2017): Characterisation of B killer cell immunoglobulin-like receptor genes and telomeric and centromeric motifs in hematopoietic stem cell transplantation donors in Vojvodina, Serbia.- Genetika, Vol 49, No.1, 345 - 354.

The outcome of HSCT is strongly inﬂuenced by the genetic similarity or identity in the HLA genes that affects the incidence of graft-versus-host disease (GvHD). Successful allogeneic HSCT, however, depends also on T-cell mediated graft-versus-leukemia (GvL) effect, in which donor-derived T cells and natural killer (NK) cells kill these malignant cells in the patient, therefore playing a crucial role in relapse prevention. The aim of this study was to make the predictive analysis of the structure and distribution of B KIR alleles and centromeric and telomeric KIR genotypes in HSCT donors in Vojvodina with regard to their contribution to protection from relapse. A total of 124 first-degree relatives of patients with hematological malignancies were examined for the presence or absence of 15 KIR genes by using of PCR-SSO technique with Luminex xMap technology. The percentage of individuals carrying each KIR gene, centromeric and telomeric KIR haplotypes and genotypes was determined by direct counting. Sixty two percent of the HSCT donors in Vojvodina carry A KIR haplotype, while nearly 38% carry B KIR haplotype. The distribution of B KIR genes showed that among 124 studied HSCT donors, 31(25%) do not carry none of the KIR genes belonging to B group, 71.77% of donors have two or more B KIR genes, 61.29% of them carry KIR 2DL2 and 2DS2 or more B KIR genes. The analysis of centromeric and telomeric KIR genotypes, showed that Cen-A1/Tel-A1 genotype had a highest frequency of 51.47% and Cen-B2/Tel-B1 the lowest frequency of 1.30%. The usage of donor KIR B gene content and centromeric and telomeric KIR gene structure could be used in development of a simple algorithm to identify donors who will provide the most protection against the relapse in related HSC transplants.

Key words: B KIR gene content, KIR motifs

Corresponding author: Svetlana Vojvodić, Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Compartment, Hajduk Veljkova 9a, 21000 Novi Sad, Serbia; Tel.: +381-21-4877-963; Fax: +381-21-4877-978;E-mail:svetlana.vojvodic021@gmail.com

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Published in „GENETIKA“ Vol. 49, No.1(2017), pp.355-364

Serbia

UDC 575
https://doi.org/10.2298/GENSR1701355O
Original scientific paper

TRICHOTHECENE CHEMOTYPE DIVERSITY OF Fusarium graminearum

ISOLATED FROM WHEAT, MAIZE AND BARLEY IN SERBIA

Ana OBRADOVIĆ¹, Slavica STANKOVIĆ¹, Vesna KRNJAJA², Ana NIKOLIĆ¹,

Dragana IGNJATOVIĆ-MICIĆ¹, Jelena STEPANOVIĆ³ and Bojan DUDUK³

¹Maze Research Institute Zemun Polje, Belgrade, Serbia

²Institute for Animal Husbandry, Belgrade, Serbia

³Institute of Pesticides and Environmental Protection, Belgrade, Serbia

Abstract

Obradović A., S. Stanković, V. Krnjaja, A. Nikolić, D. Ignjatović-Micić, J. Stepanović and B. Duduk (2017): Trichothecene chemotype diversity of Fusarium graminearum isolated from wheat, maize and barley in Serbia.- Genetika, Vol 49, No.1., 355-364.

Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15-ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i.e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.

Key words: Fusarium graminearum, trichothecene chemotype, NIV, 15-ADON, 3-ADON

Corresponding author: Slavica Stanković, Maize research institute, S.Bajica 1, 11185 Belgrade, Serbia, e-mail: sstojkov@mrizp.rs

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Published in „GENETIKA“ Vol. 49, No.1(2017), pp.365-376

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1701365S
Original scientific paper

A MULTIVARIATE APPROACH TO THE SELECTION OF PEA (Pisum sativum L.) LINES OBTAINED BY THE SINGLE SEED DESCENT TECHNIQUE

Maria SURMA¹, Tadeusz ADAMSKI¹, Zygmunt KACZMAREK¹, Wojciech ŚWIĘCICKI^1,2, Stanisław MEJZA³, Paweł BARZYK², Anetta KUCZYŃSKA¹, Karolina KRYSTKOWIAK¹, Krzysztof MIKOŁAJACZAK¹, Piotr OGRODOWICZ¹

¹Institute of Plant Genetics of the Polish Academy of Sciences Poznań, Poland

²Poznań Plant Breeding Ltd., Tulce, Poland

³ Poznań University of Life Sciences, Poznań, Poland

Abstract

Surma M., T. Adamski, Z. Kaczmarek, W. Święcicki, S. Mejza, P. Barzyk, A. Kuczyńska, K. Krystkowiak, K. Mikołajaczak, P. Ogrodowicz (2017): A multivariate approach to the selection of pea (Pisum sativum L.) lines obtained by the single seed descent technique.- Genetika, Vol 49, No.1., 365-376.

Grain legumes, especially pea and lupine, are important agricultural crops in Central and Northern Europe. Because of the high level of protein in their seeds, these species constitute an alternative to soya meal imported from South America. The breeding of new cultivars in a short time is a major problem in pea breeding, particularly since the production of homozygous lines in the DH (doubled haploid) system, as used for cereals, for example, is very difficult. An alternative approach may be the single seed descent (SSD) method. The materials for this study were lines of pea obtained by that method combined with in vitro culture of embryos. This approach enabled 9 generations to be obtained in 2.5 years. First, SSD lines (F₁₀) were investigated in a field experiment conducted in a complete randomized design with two replications, in which the dates of flowering and ripening and seed yield per plant were observed. Seventy seven lines out of 120 (64%) were selected for a subsequent experiment in which F₄ generations of each cross combination were also included. In the second field experiment SSD lines were evaluated for earliness, plant height, seed yield per plot, and 1000-seed weight. The data were processed by multivariate analysis of variance and related methods, leading to the selection of 16 lines for further breeding experiments. Multivariate analysis can be helpful for breeders in seeking plants with a new favorable complex of yield-forming traits.

Key words: embryo in vitro culture, homozygous lines, pea breeding, pre-selection

Corresponding author: Stanisław Mejza, Poznań University of Life Sciences, Department of Mathematical and Statistical Methods, Wojska Polskiego 28, 60-637 Poznań, Poland, smejza@up.poznan.pl, tel. +48505547683

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