GENETIKA, Vol

GENETIKA, Vol. 49, No. 2(2017)

Jelena DJUROVIC, Oliver STOJKOVIC, Jelena TODOROVIC, Kristina SAVIC, Gorana STAMENKOVIC

SHOULD MTHFR 1298 A>C BE TESTED TOGETHER WITH MTHFR 677 C>T POLYMORPHISM IN WOMEN WITH REPRODUCTIVE CHALLENGES? [Abstract] [Full text]

Jasna BOŠNJAK-NEUMÜLLER, Ninoslav DJELIĆ, Milena RADAKOVIĆ, Stoimir KOLAREVIĆ, Dragana MITIĆ-ĆULAFIĆ, Zora DAJIĆ-STEVANOVIĆ, Branka VUKOVIĆ-GAČIĆ, Jelena KNEŽEVIĆ-VUKČEVIĆ, Zoran STANIMIROVIĆ

GENOTOXICITY OF TRIIODOTHYRONINE: EFFECTS ON Salmonella typhimurium TA100 AND HUMAN LYMPHOCYTES in vitro [Abstract] [Full text]

Georgi antov, Maria krasteva, Silvia andonova, Alexey savov, Svetla angelova, Lubomir stoilov, Draga toncheva

STK11 gene Mutations among patients with sporadic breast cancer [Abstract] [Full text]

Saida hasanova, Zeynal akparov, Alamdar mammadov, Litfer amirov, Sevda babayeva, Jale nasibova, Zumrud mukhtarova, Kamila shikhaliyeva, Vusala izzatullayeva, Mehraj abbasov

GENETIC DIVERSITY OF CHICKPEA GENOTYPES AS REVEALED BY ISSR AND RAPD MARKERS [Abstract] [Full text]

Mehrab yadegari and Mohammad Hosein ANSARI

STUDY OF MULTIVARIATE ANALYSIS OF QUANTITATIVE TRAITS IN IRANIAN PUMPKIN LINES [Abstract] [Full text]

Yalçın COŞKUN and İsmail TAŞ

Respons of wheat species to irrigation water salinity [Abstract] [Full text]

Veselin draganić, Jelena lozo,Marjan biočanin, Ivica dimkić, Eliana Garalejić, Djordje fira, Slaviša stanković,Tanja berić

GENOTYPING OF BACILLUS SPP. ISOLATE COLLECTION FROM NATURAL SAMPLES [Abstract] [Full text]

Milena ugrin^*, Iva milacic^*, Anita skakic, Kristel klaassen, Jovana komazec, Sonja pavlovic, Maja stojiljkovic

MOLECULAR GENETIC STRATEGY FOR DIAGNOSIS OF CONGENITAL ADRENAL HYPERPLASIA IN SERBIA [Abstract] [Full text]

Ali mohammadi and Mohammad farhadian

GENETIC EVALUATION OF GROWTH TRAITS IN IRANIAN KORDI SHEEP USING RANDOM REGRESSION MODEL WITH HOMOGENEOUS AND HETEROGENEOUS RESIDUAL VARIANCES [Abstract] [Full text]

Valiollah SOLEIMANI, Jafar AHMADI, Behzad SADEGHZADEH, Saber GOLKARI

THE EXPRESSION OF GMP5CS, GMPAP3, AND GMBZIP50 GENES UNDER SALINE CONDITION IN SOYBEAN USING REAL-TIME PCR [Abstract] [Full text]

Elahe saroei, Kianoosh cheghamirza, Leila zarei

GENETIC DIVERSITY OF CHARACTERISTICS IN BARLEY CULTIVARS [Abstract] [Full text]

Imren kutlu, Alpay balkan, Kayıhan Z. korkut, Oguz bilgin, Ismet baser

EVALUATION OF RECIPROCAL CROSS POPULATIONS FOR SPIKE-RELATED TRAITS IN EARLY CONSECUTIVE GENERATIONS OF BREAD WHEAT (Triticum aestivum L.) [Abstract] [Full text]

Branislava BATOS, Danijela MILJKOVIĆ, Marko PEROVIĆ, Saša ORLOVIĆ

MORPHOLOGICAL VARIABILITY OF QUERCUS ROBUR L. LEAF IN SERBIA[Abstract] [Full text]

Somayeh Esfandani bozchaloyi, Masoud sheidai, Maryam keshavarzi, Zahra noormohammadi

GENETIC DIVERSITY AND MORPHOLOGICAL VARIABILITY IN Geranium purpureum VILL. (GERANIACEAE) OF IRAN [Abstract] [Full text]

Jelena filipović tričković, Vesna mandušić, Ivana joksić, Dragana vujić, Ana valenta šobot, Gordana joksić

GENOTYPING FANCONI ANEMIA PATIENTS FROM SERBIA REVEALS THREE NOVEL FANCD2 VARIANTS [Abstract] [Full text]

Emina mladenović, Sandra cvejić, Siniša jocić, Jelena čukanović, Milan jocković, Goran malidža

VARIABILITY OF MORPHOLOGICAL CHARACTERS AMONG ORNAMENTAL SUNFLOWER COLLECTION [Abstract] [Full text]

Sonja petrović, Sonja marić,Tihomir čupić, A. rebekić, Ivana rukavina

ASSESSMENT OF MOLECULAR AND PHENOTYPIC DIVERSITY AMONG WINTER WHEAT CULTIVARS [Abstract] [Full text]

Olivera milošević-djordjević, Darko grujičić, Marina radović jakovljević, Dragoslav marinković, Sladjana dimitrijević, Olgica mihaljević, Ljiljana mijatović-teodorović and Snežana živančević –simonović

INFLUENCE OF GSTT1 AND GSTM1 NULL GENOTYPES ON DIFFERENTIATED THYROID CANCER RISK AND BASELINE AND RADIOIODINE INDUCED CYTOGENETIC DAMAGE IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS [Abstract] [Full text]

Azra skender, Mirsadkurtović, Narispojskić, Belma kalamujić STROIL, Semina hadžiabulić, Fuad gaši

GENETIC STRUCTURE AND DIVERSITY OF EUROPEAN CHESTNUT (Castanea sativa MILL.) POPULATIONS IN WESTERN BALKANS: ON A CROSSROAD BETWEEN EAST AND WEST [Abstract] [Full text] [Supplement]

Jainikkumar PATEL and Jenabhai CHAUHAN

EVALUATION OF DGAT1-EXON 8 K232A SUBSTITUTION IN GIR AND KANKREJ (Bos indicus), INDIAN ORIGIN CATTLE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS [Abstract] [Full text]

Marija MILIVOJEVIĆ, Ana NIKOLIĆ, Ksenija MARKOVIĆ, Milomir FILIPOVIĆ, Jelena VANČETOVIĆ, Tanja PETROVIĆ, Jelena SRDIĆ

RELATIONSHIP BETWEEN GENETIC DIVERSITY AND COLD-TOLERANCE OF MAIZE INBRED LINES [Abstract] [Full text]

Stefan Tsonev, Velichka Todorova, Stanislava Groseva, Teodora Popova, Elena G. Todorovska

EVALUATION OF DIVERSITY IN BULGARIAN PEPPER CULTIVARS BY AGRONOMICAL TRAITS AND ISSR MARKERS [Abstract] [Full text]

Mihajlo ćirić, Živko ćurčić, Milan mirosavljević, Ana marjanović jeromela,Goran jaćimović, Slaven prodanović, Tomislav živanović
ASSESSMENT OF SUGAR BEET ROOT YIELD BY AMMI ANALYSIS [Abstract] [Full text]

Jisu WU, Xiaobin L U, Zitong YU, Caixia HAN, Xiaohui LI, Slaven PRODANOVIC, Yueming YAN

CORRELATION AND PATH ANALYSIS FOR GRAIN YIELD AND YIELD COMPONENTS IN CHICKPEA (Cicer arietinum L.) [Abstract] [Full text]

Hasan Pinar, Ercan Yildiz, Mustafa Kaplankiran, Celil Toplu, Mustafa Unlu, Sedat Serce, Sezai Ercisli

MOLECULAR CHARACTERIZATION OF SOME SELECTED PERSIMMON GENOTYPES AND CULTIVARS BY SRAP AND SSR MARKERS [Abstract] [Full text]

HOJJAT HASHEMINASAB and MOHAMMAD TAGHI ASSAD

GENETIC AND MULTIVARIATE PHENOTYPIC ANALYSES OF SOME SELECTION INDICES IN PISTACHIO (Pistacia vera L.) CULTIVARS UNDER DROUGHT STRESS CONDITIONS [Abstract] [Full text]

Kameh ABOOKAZEMI, Mokhtar JALALI JAVARAN, Mehdi MOHEBODINI, and Akbar VASEGHI
TRANSFER OF HUMAN PROINSULIN GENE INTO CUCUMBER (Cucumis sativus L.) VIA AGROBACTERIUM METHOD [Abstract] [Full text]

Nenad Pavlović, Zdenka Girek, Milan Zdravković, Jelica Gvozdanović Varga, Đorđe Moravčević, Jasmina Zdravković

MODE OF INHERITANCE and AMMI analysis of onion (Allium cepa L.) bulb traits [Abstract] [Full text]

Abel Olusegun OGUNTUNJI

REGRESSION TREE ANALYSIS FOR PREDICTING BODY WEIGHT OF NIGERIAN MUSCOVY DUCK (Cairina moschata) [Abstract] [Full text] [Supplement]

Li-jing CHEN, Fu-xing CHENG, Shao-kun SUN, Jun SUN, Muhammad IRFAN,Li ZHANG

AN OVERVIEW OF MOLECULAR GENETIC LINKAGE MAPS IN Lilium SPP. [Abstract] [Full text]

Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 377-386

UDC 575
https://doi.org/10.2298/GENSR1702377D

Orginal scientific paper

SHOULD MTHFR 1298 A>C BE TESTED TOGETHER WITH MTHFR 677 C>T POLYMORPHISM IN WOMEN WITH REPRODUCTIVE CHALLENGES?

Jelena DJUROVIC^1*, Oliver STOJKOVIC¹, Jelena TODOROVIC², Kristina SAVIC¹,

Gorana STAMENKOVIC³

¹Institute of Forensic Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

²Specialized medical practice in the field of internal medicine "Teamed", Belgrade, Serbia

³Institute of Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia

Abstract

Djurovic J., O. Stojkovic, J. Todorovic, K. Savic, G. Stamenkovic (2017): Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?.- Genetika, vol 49, no.2, 377-386.

Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C>T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A>C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C>T and 1298A>C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C>T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p>0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C>T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A>C) should be considered, since we have shown that it dramatically increases the rate of detection of patients who are potentially at risk for MTHFR associated conditions.

Keywords: MTHFR polymorphisms, 677C>T and 1298A>C, testing together, pregnancy

Corresponding author: Jelena Djurovic,Institute of Forensic Medicine, Faculty of Medicine, University of Belgrade,Deligradska 31a, 11000 Belgrade, Serbia,Tel: +381 11 3617931; Fax: +381 11 3617931;E-mail: djurovic_j@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 387-397

Serbia

UDC 575
https://doi.org/10.2298/GENSR1702387B

Original scientific paper

GENOTOXICITY OF TRIIODOTHYRONINE: EFFECTS ON Salmonella typhimurium TA100

AND HUMAN LYMPHOCYTES in vitro

Jasna BOŠNJAK-NEUMÜLLER¹*, Ninoslav DJELIĆ¹*, Milena RADAKOVIĆ¹,

Stoimir KOLAREVIĆ², Dragana MITIĆ-ĆULAFIĆ², Zora DAJIĆ-STEVANOVIĆ³,

Branka VUKOVIĆ-GAČIĆ², Jelena KNEŽEVIĆ-VUKČEVIĆ², Zoran STANIMIROVIĆ²

¹Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Serbia

²Department of Microbiology, Faculty of Biology, University of Belgrade, Serbia

³Department of Crop Science, Faculty of Agriculture, University of Belgrade, Serbia

Abstract

Bošnjak-Neumüller J., N. Djelić, M. Radaković, S. Kolarević, D. Mitić-Ćulafić, Z. Dajić-Stevanović, B. Vuković-Gačić, J. Knežević-Vukčević, Z. Stanimirović (2017): Genotoxicity of Triiodothyronine: effects on Salmonella typhimurium TA100 and human lymphocytes in vitro.- Genetika, vol 49, no.2, 387 -397.

There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T₃). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T₃ concentrations was applied. The obtained results showed absence of genotoxic effects of T₃ in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 µM. We only observed cytotoxic effects in bacterial system at very high T₃ concentrations (300 and 500 µM). In conclusion, T₃ was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T₃ in mammalian cells.

Keywords: Comet assay, DNA damage, human lymphocytes, triiodothyronine, TA100 Salmonella typhimurium

Corresponding author: Ninoslav Djelić, Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Oslobodjenja Blvd. 18,11000 Belgrade, Serbia, Phone: +381 11 265 88, 94, Fax: +381 11 268 59 36, E-mail: ndjelic@vet.bg.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp.399-413

Serbia

UDC 575
https://doi.org/10.2298/GENSR170399A

Original scientific paper

STK11 gene Mutations among patients with sporadic breast cancer

Georgi antov¹, Maria krasteva¹, Silvia andonova², Alexey savov²,

Svetla angelova¹, Lubomir stoilov¹, Draga toncheva³

¹Laboratory of Genome dynamics and stability, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria,

²National Genetic Laboratory, UHOG “Maichin dom”, Medical University Sofia, Sofia, Bulgaria,

³Department of Medical Genetics, Faculty of Medicine, Medical University Sofia, Sofia, Bulgaria

Abstract

antov G., M. krasteva, S. andonova, A. savov, S. angelova, L. stoilov, D.toncheva (2017): STK11 gene mutations among patients with sporadic breast cancer.- Genetika, vol 49, no. 2, 399 - 413.

Germline mutations affecting STK11 (LRG_319) are profoundly studied in relation to Peutz-Jeghers syndrome, predisposing to the development of various cancers at multiple sites. Though somatic mutations in STK11 are found to be present in several cancers, limited data on its involvement in sporadic breast cancer are available. The present study aims to evaluate the frequency and spectrum of genetic alterations in STK11 in a group of Bulgarian patients with sporadic breast cancer. A total of 73 tumor and 22 corresponding blood specimens derived from the patients, and 10 blood samples from clinically healthy controls were analyzed. High Resolution Melting analysis followed by Sanger sequencing and bioinformatic prediction tools were utilized. Seven patients (9.58%) harbored STK11 alterations, only two (2.74%) of which are exonic: one nonsense c.322A>T; p.K108X (deleterious) and one missense c.440G>A; p.Arg147His (of unknown significance). Two intronic variants were also observed: c.290+36G>T and c.*16+18C>A (novel). To our knowledge the results represent the first data indicating presence of STK11 alterations in patients with sporadic breast cancer. The limited number of the detected deleterious mutations indicates that mutational inactivation of the gene is a rare event and probably plays a minor role in sporadic breast carcinogenesis.

Keywords: High Resolution Melting Analysis, Mutation, Sporadic breast cancer, STK11, Tumor suppressor gene

Corresponding author: Georgi Georgiev Antov, Laboratory of Genome dynamics and stability, Institute of Plant physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113, Sofia, Bulgaria, Tel.: ++359 2 9792617, Fax: ++359 2 8739952, Mobile: ++359 895020134, E-mail: antov8107@abv.bg

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Published in „GENETIKA“ Vol. 49, No2 (2017), pp. 415-423

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR17012415H

Original scientific paper

GENETIC DIVERSITY OF CHICKPEA GENOTYPES AS REVEALED BY ISSR AND RAPD MARKERS

Saida hasanova¹, Zeynal akparov¹, Alamdar mammadov², Litfer amirov¹, Sevda babayeva¹, Jale nasibova¹, Zumrud mukhtarova¹, Kamila shikhaliyeva¹, Vusala izzatullayeva¹, Mehraj abbasov^1,3

¹Genetic Resources Institute of National Academy of Sciences of Azerbaijan, Baku, Azerbaijan

²Institute of Molecular Biology and Biotechnologies of ANAS, Baku, Azerbaijan

³Baku State University, Baku, Azerbaijan

Abstract

hasanova S., Z. akparov, A. mammadov, L. amirov, S. babayeva, J. nasibova, Z. mukhtarova, K. shikhaliyeva, V. izzatullayeva, M. abbasov (2017): Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers.- Genetika, vol 49, no. 2, 415 -423.

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11 RAPD primers. In the study RAPD primers detected more polymorphism (98%) than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR and 0.85 for RAPD) for each of these marker systems. Cluster analysis performed from both separate and combined data of RAPD and ISSR markers using SPSS software package. Jaccard’s similarity coefficient for 62 chickpea genotypes was 0.65. Cluster analyses based on combined data generated a dendrogram that separated genotypes into 11 clusters. Four clusters contained only one genotype showing the genetic uniqueness of these accessions. The studied chickpea collection has been proved to constitute a rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing between distantly related genotypes is expected to yield more vigorous plants constituting much of the different traits contained in the two parental lines.

Keywords: chickpea, dendrogram, genetic diversity, ISSR, RAPD

Corresponding author: Mehraj Abbasov, Genetic Resources Institute of National Academy of Sciences of Azerbaijan, Azadliq Ave 155, AZ1106, Baku, Azerbaijan, e-mail: mehraj_genetic@yahoo.com

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp. 425-433

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702425Y

Original scientific paper

STUDY OF MULTIVARIATE ANALYSIS OF QUANTITATIVE TRAITS IN IRANIAN PUMPKIN LINES

Mehrab yadegari¹ and Mohammad Hosein ANSARI²

¹Department of Agronomy and Medicinal Plants, Faculty of Agriculture, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

²Department of Agronomy and Breeding Crops, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract

yadegari M. and M. H. Ansari (2017): Study of multivariate analysis of quantitative traits in Iranian pumpkin lines.-Genetika, vol 49, no.2, 425-433.

In this study, seed yield production and its different components fruit length, fruit diameter, fruit length/fruit diameter ratio (FL/FD), diameter of flesh, diameter of seed core, fruit weight, weight of 1000 seed from 24 lines of pumpkin grown in Iran was examined. Twenty-five characters in all plant lines were measured by Descriptor (UPOV) and data were subjected to cluster analysis. Results showed that plants lines were divided in four groups. In all groups, regression comparisons were made for modeling the effect of different characters on seed yield, results also showed that fruit weight and fruit length in all groups had the most direct effect on seed yield. In conclusion, these traits are suggested as the best indirect selection criteria to improve the seed yield genetically in Cucurbita spp. genotypes especially in preliminary generation of breeding and selection programs.

Keywords: Cucurbita pepo, fruit characteristics, path analysis, seed yield

Corresponding author: Mehrab yadegari, Department of Agronomy and Medicinal Plants, Faculty of Agriculture, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. Fax: +98 38333361093-mehrabyadegari@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 435-444

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1702435C

Original scientific paper

Respons of wheat species to irrigation water salinity

Yalçın COŞKUN¹ and İsmail TAŞ²

¹Çanakkale Onsekiz Mart University, Lapseki Vocational College, Çanakkale, Turkey

²Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Agricultural Structures and Irrigation, Çanakkale, Turkey

Abstract

Coşkun Y. and İ. Taş (2017): Respons of wheat species to irrigation water salinity.- Genetika, vol 49, no2, 435 - 444.

This study was conducted to investigate the effects of different irrigation water salinity level on chromosomes and shoots of wheat with three different ploidy level (diploid, tetraploid and hexaploid). Greenhouse experiment revealed that irrigation water salinity level had significant effects on shoot dry weight, root dry weight, shoot length and root length (P<0.05). The effects of ploidy level and cultivar shoot dry weight and root dry weight were also found to be significant (P<0.05), but the effects on shoot length and root length were not significant (P>0.05). Negative effects of salinity on shoot and root were started at 8 dS m^-1. Also hexaploid wheat was more tolerant then tetraploid and diploid wheat to salinity. It was not determined that possible effects of irrigation water salinity to structure of chromosomes with current equipment and methods. Cell divisions were normal, but decreasing cell division rates were observed with increasing irrigation water salinity levels.

Keywords: ploidy levels, water salinity, wheat

Corresponding author: Yalçın Coşkun, Çanakkale Onsekiz Mart University, Lapseki Vocational College, 17800 Çanakkale, Turkey, Phone: +90 286 522 61 04,Fax: +90 286 522 61 01,e-mail: ycoskun33@hotmail.com

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp.445-456

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702445D

Original scientific paper

GENOTYPING OF BACILLUS SPP. ISOLATE COLLECTION FROM NATURAL SAMPLES

Veselin draganić¹, Jelena lozo²,Marjan biočanin^2,3, Ivica dimkić²,

Eliana Garalejić^1,4, Djordje fira², Slaviša stanković²,Tanja berić^2*

¹Obstetrics and Gynaecology Clinic „Narodni front”, Belgrade, Serbia

²Faculty of Biology, University of Belgrade, Belgrade, Serbia

³Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Institute for Bioengineering, Laboratory of Systems Biology and Genetics, Lausanne, Switzerland

⁴Faculty of Medicine, University of Belgrade, Belgrade, Serbia

Abstract

draganić V., J. lozo,M. biočanin, I. dimkić, E. Garalejić, Dj. fira, S. stanković,T. berić (2017): Genotyping of Bacillus spp. isolate collection from natural samples.- Genetika, vol 49, no2, 445 - 456.

The aim of this study was genotyping and identification of collection of 164 Bacillus spp. isolates, from samples of soil, manure, and straw gathered from across Serbia, using Pulse field gel electrophoresis (PFGE) combined with sequencing of tuf gene, one of the housekeeping genes. The PFGE analysis with NotI enzyme was used to determine phylogenetic relationships of isolates and referent strains. Four large groups of Bacillus spp. were distinguishable: cereus, subtilis, pumilus and megaterium and within enormous genetic diversity. Bacillus subtilis Marburg referent strain did not group with rest of the strains from the subtilis group (Bacillus subtilis ATCC6633 and Bacillus atrophaeus ATCC9372). Strains from the cereus group were distinguished and closely grouped together. One representative isolate from each of 21 distinct PFGE groups was identified by sequencing of tuf gene. Eight different species were identified among chosen isolates: B. amyloliquefaciens, B. subtilis, B. pumilus, B. safensis, B. megaterium, B. cereus, B. anthracis and B. thuringiensis. Our results showed that PFGE analysis combined with sequencing of one of the housekeeping genes could be used for characterization of large collections of Bacillus isolates. The determination of tuf gene recommended itself to be an adequate and sufficient analysis for obtaining very clear and unambiguous results, with high resolution of separation of Bacillus species.

Keywords: Bacillus identification, PFGE, tuf gene

Corresponding author: Tanja Berić, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia, Tel/Fax: +381 11 2637364, E-mail address: tanjab@bio.bg.ac.rs

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp.457-467

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702457U

Original scientific paper

MOLECULAR GENETIC STRATEGY FOR DIAGNOSIS OF CONGENITAL ADRENAL HYPERPLASIA IN SERBIA

Milena ugrin^*, Iva milacic^*, Anita skakic, Kristel klaassen, Jovana komazec,

Sonja pavlovic, Maja stojiljkovic

Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

Abstract

ugrin M., I. milacic, A. skakic, K. klaassen, J. komazec, S. pavlovic, M. stojiljkovic (2017): Molecular genetic strategy for diagnosis of congenital adrenal hyperplasia in Serbia.- Genetika, vol 49, no2, 457 - 467.

Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency is one of the most common endocrine diseases, yet genetic diagnosis is among the most complicated of all monogenic disorders. It has an overall incidence of 1:10000-1:20000, it is inherited in autosomal recessive pattern and caused by mutations affecting CYP21A2 gene. Based on the phenotypic expression, this disease is categorized into severe, classical form revealed at birth and mild, non-classical form. Although diagnosis could be established based on biochemical tests and distinctive clinical features, molecular genetic testing is crucial for diagnosis confirmation, detection of carriers and asymptomatic patients, disease prognosis, as well as for providing proper genetic counselling and prenatal diagnosis. Based on CYP21A2 mutational spectrum and frequencies in Serbia, in this paper we propose an optimal molecular genetic diagnostic algorithm for CAH and discuss genetic mechanisms underlying the disease. The complete diagnostic procedure combines multiplex minisequencing technique (SNaPshot PCR) as a method for rapid detection of common point mutations, direct sequencing of whole CYP21A2 gene and PCR with sequence specific primers (PCR-SSP) for large gene rearrangements detection (CYP21A1P/CYP21A2 chimeras). While SNaPshot PCR assay analyses ten common mutations (c.290-13A/C>G, p.P30L, p.R356W, p.G110fs, p.V281L, p.Q318X, p.L307fs, p.I172N, Cluster p.[I236N;V237E;M239K] and p.P453S) which account for over 80% of all CYP21A2 mutations in Serbian population, direct sequencing of CYP21A2 gene is needed to identify potential rare or novel mutations present in Serbian population with frequency of 1.8%. Additionally, large gene rearrangements which are present with frequency of 16.7% make PCR-SSP analysis an unavoidable part of molecular characterization of CAH in Serbia. Described molecular genetic strategy is intended to facilitate correct diagnosis assessment in CAH affected individuals and their families in Serbia but it will also contribute to molecular genetic testing of CAH patients across Europe.

Keywords: Congenital adrenal hyperplasia, CYP21A2, CYP21A1P/CYP21A2 chimeras, molecular genetic diagnostic algorithm, SNaPshot PCR

Corresponding author: Maja Stojiljkovic, PhD, Senior Research Associate, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade 11010, Serbia, Tel: +381 11 3976 445; Fax: +381 11 3975 808, E-mail: maja.stojiljkovic@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp. 469-482

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702469M

Original scientific paper

GENETIC EVALUATION OF GROWTH TRAITS IN IRANIAN KORDI SHEEP USING RANDOM REGRESSION MODEL WITH HOMOGENEOUS AND HETEROGENEOUS RESIDUAL VARIANCES

Ali mohammadi¹ and Mohammad farhadian²*

¹Young Researchers and Elite Club, Hamedan Branch, Islamic Azad University, Hamedan, Iran

²Young Researchers and Elite Club, Khoy Branch, Islamic Azad University, Khoy, Iran

Abstract

mohammadi A. and M. farhadian (2017): Genetic evaluation of growth traits in Iranian Kordi sheep using random regression model with homogeneous and heterogeneous residual variances.- Genetika, vol 49, no. 2, 469 - 482.

The purpose of this study was estimation of genetic parameter using random regression model (RRM) with various error variance in Iranian Kordi sheep. The data (consisting of 7875 weight records from birth to 360 days of age) were collected during the period 2000 to 2013 from the rearing and breeding station of Kordi sheep in Shirvan, Iran. The independent variables were Legendre polynomials (LP) of age at weighing and orders of fit from 2 to 5 were considered. Analyses were carried out fitting sets of random regression coefficients due to direct additive genetic, direct and maternal permanent environmental effects, with heterogeneous and homogeneous error variances. To compare the model were used different criteria such as LogL, AIC, BIC and LRT. The best fitting RRM among homogeneous error variance was the model with a LP of fourth order for fixed effect, fourth order for direct additive genetic and fifth order for direct and maternal permanent environmental effects (model 4455). Among the models with heterogeneous error variances different, model 7 (Heterogeneous error variances of 72 various classes), was selected as the best model. The variances increased along the trajectory from 3.75 to 12.81, 4.43 to 30.28 and 1.49 to 8.49; 0.25 to 27.94, 0.03 to 12.32 and 0.15 to 22.66 for direct additive genetic, direct and maternal permanent environmental effect by homogeneous and heterogeneous error variances, respectively. The direct heritability ranged from 0.15 to 0.41 and 0.11 to 0.56 by homogeneous and heterogeneous error variances, respectively. Genetic correlation between adjacent test days was more than between distant test days. This research has demonstrated the possibility of application of RRM with heterogeneous error variance for genetic evaluation of Iranian Kordi Sheep.

Keywords: body weight, genetic parameters, random regression model, sheep

Corresponding author: Mohammad Farhadian, Tel.: +98 918 813 5839; fax: +98 813 450 6128.

E-mail address: Mohammad.farhadian@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 483-494

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702483S

Original scientific paper

THE EXPRESSION OF GMP5CS, GMPAP3, AND GMBZIP50 GENES UNDER SALINE CONDITION IN SOYBEAN USING REAL-TIME PCR

Valiollah SOLEIMANI^1*, Jafar AHMADI², Behzad SADEGHZADEH³, Saber GOLKARI³

¹Department of Agricultural Biotechnology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

²Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran

³Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization, Maragheh, Iran

Abstract

Soleimani V., J. Ahmadi, B. Sadeghzadeh, S. Golkari (2017): The expression of GMP5CS, GMPAP3, and GMBZIP50 genes under saline condition in soybean using real-time PCR.- Genetika, vol 49, no2, 483-494.

Salinity in higher plants is the cause of toxicity and osmotic stress. To identify the expression pattern of salinity tolerance genes in soybean, it was investigated the relative expression of three genes, GmPAP3, GmBZIP50 and GmP5CS at two tissues leaf and root in Williams (as tolerant) and L17 (as susceptible) soybean genotypes under two levels zero (control) and 300 Mm NaCl. For adaptation of plantlets to salinity, salinity was started with 150 mM NaCl for first time and followed by 300 mM NaCl in two times. The housekeeping gene 18SrRNA was used to normalize data. Data analysis showed that all three genes expression increased under salinity stress. The expression of GmP5CS and GmBZIP50 was two-fold greater in Williams than in L17 genotype, while GmPAP3 expression was 2.5-fold greater in L17 than in Williams. The expression of GmP5CS and GmBZIP50 was higher in roots than in leaves, while GmPAP3 expression was higher in leaves than in roots. In conclusion, GmBZIP50 over-expressed more than two other genes by 375 and 273% in leaves and roots under stressed compared to non-stressed plants. Thus GmBZIP50 could be more effective candidate gene for producing soybeans with resistance to salinity that may be resulted from transferring and increasing copy number.

Keywords: gene expression, GmPAP3, GmBZIP50, GmP5CS, salinity

Corresponding author: V. Soleimani, Maragheh Branch, Islamic Azad University, Maragheh, Iran, POX: 551974591, Tel: +98 2833901130; Fax +98 2833780073, soleimani.vikiu@gmail.com, soleimani_vikiu@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 495-510

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1702495S

Original scientific paper

GENETIC DIVERSITY OF CHARACTERISTICS IN BARLEY CULTIVARS

Elahe saroei¹, Kianoosh cheghamirza¹, Leila zarei¹

¹Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Abstract

saroei E., K. cheghamirza, L.zarei (2017): Genetic diversity of characteristics in barley cultivars.- Genetika, vol 49, no2, 495-510.

Knowledge of the genetic diversity is important to design appropriate selection criteria in the breeding programs. In this study, the genetic diversity of 42 Iranian and European barley cultivars was investigated with morpho-agronomic traits including phenological, physiological, morphological traits, grain yield and associated traits. Analysis of variance showed high variability among cultivars. The European cultivars Panaka, Aiace and Pariglia had the highest grain yield. The results of group comparisons indicated that the European cultivars produced higher grain yield (500.57 g/m²) than the Iranian cultivars (445.50 g/m²), but larger genetic diversity based on morpho-agronomic traits was observed among Iranian cultivars than European cultivars. Correlation analysis revealed the high significant correlations between grain yield with biological yield (0.92), straw yield (0.77), and number of spike per square meter (0.67). Based on the factor analysis, the six factors that justified 81.63 percent of the variations were identified. The first factor having the largest eigenvalue was identified as effective factor on the vegetative and reproductive growth. In path analysis, biological yield had the greatest effect on grain yield (0.906). Cluster analysis classified the cultivars in six groups and showed that genetic variation based on the all studied traits among the barley cultivars was not related to geographical location.

Keywords: Barley, genotype diversity, group comparisons, traits similarity.

Corresponding author: Kianoosh Cheghamirza, Department of Agronomy and Plant Breeding, Razi University, Kermanshah, 6715685438, Iran, E-mail: cheghamirza@yandex.ru, Tel: +98 8338323731; Mobile No: +98 9188311085; Fax No: +98 83338323731

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp. 511-528

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1702511K

Original scientific paper

EVALUATION OF RECIPROCAL CROSS POPULATIONS FOR

SPIKE-RELATED TRAITS IN EARLY CONSECUTIVE GENERATIONS

OF BREAD WHEAT (Triticum aestivum L.)

Imren kutlu^1*, Alpay balkan², Kayıhan Z. korkut², Oguz bilgin²,

Ismet baser²

¹Eskisehir Osmangazi University, Faculty of Agriculture, Department of Biosystem Engineering, Eskisehir, Turkey

²Namik Kemal University, Faculty of Agriculture, Department of Field Crops, Tekirdag, Turkey

Abstract

kutlu I., A.balkan, K. Z. korkut, O.bilgin, I. baser (2017): Evaluation of reciprocal cross populations for spike-related traits in early consecutive generations of bread wheat (Triticum aestivum L.).- Genetika, vol 49, no2, 511-528.

Breeding effort on increasing grain yield of wheat will incessantly continue because it is indispensable product. Obtaining the genetic information such as genotypic variation, heritability, genetic advance is the fundamental components of these studies. It is important that the maternal effects are put forward throughout successive generations because of genotypic and/or environmental effects as far as variation. This research was conducted to investigate changes of reciprocal crosses throughout successive generations and determine selection criteria for high yield in early generations. For this purpose, the populations were analyzed with regard to genotypic and phenotypic variation coefficient, heritability, genetic advance and Unweighted Pair Group Method (UPGMA) cluster analysis for real crosses, reciprocals and all genotypes separately. According to the results, heritability and genetic advance values of traits investigated were highly varied throughout successive generations among real crosses, reciprocals and all genotypes. This finding indicated that non-additive gen effects or epitasis played a role in inheritance of all traits. Dissimilarity of crosses than their reciprocals indicated variation of successive generation. Dissimilarity value of each parent differed as generation progresses according to combination created. This condition suggested that there were maternal effects in this population throughout successive generations. Grain weight per spike, spike harvest index and spike density had high direct and indirect effects on the grain yield at all of three generations, it proved that these traits can be a selection criterion for early generations. Sana was the best parent and ‘Bezostaja x Krasunia’ and ‘Krasunia x Pehlivan’ were best performance in most of traits at all generations.

Keywords:cluster analyses, early generation, genotypic effects, reciprocal crosses, spike traits

Corresponding author: Imren Kutlu, Eskisehir Osmangazi University, Faculty of Agriculture, Department of Biosystem Engineering, 26160 Eskisehir, Turkey, E-mail: ikutlu@ogu.edu.tr, Phone: +90 222 3242990, Fax: +90 222 3242991

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 529-541

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702529B

Original scientific paper

MORPHOLOGICAL VARIABILITY OF QUERCUS ROBUR L. LEAF IN SERBIA

Branislava BATOS ^1*, Danijela MILJKOVIĆ ², Marko PEROVIĆ ³, Saša ORLOVIĆ ⁴

¹*Institute of Forestry, Belgrade, Serbia

² Institute for Biological Research ”S. Stanković” University of Belgrade, Belgrade, Serbia

³Faculty of Forestry University of Belgrade, Belgrade, Serbia

⁴Faculty of Agriculture University of Novi Sad, Novi Sad, Serbia

Abstract

Batos B., D. Miljković, M. Perović, S. Orlović (2017): Morphological variability of Quercus robur L. leaf in Serbia.- Genetika, vol 49, no2, 529- 541.

This paper presents the results of a study dealing with leaf morphological variability of Quercus robur L. 148 trees were sampled from 5 population across Serbia and 17 morphological traits were assessed. Interpopulation variability was confirmed by the results of the analysis of variance (ANOVA). A statistically significant (p <0.05) effect of population was obtained for most of the studied morphological characters. Intrapopulation variability was confirmed by statistically significant tree effects for all of the studied leaf characters (all p < 0.05). The results of the multivariate analysis of variance (MANOVA) confirmed a significant population and tree share in the total phenotypic variability (all p <0.05). By applying the canonical discriminant analysis (CDA), the first discriminant function accounted for 63% of the variability between populations and the second accounted for 20% of the population variability. The leaf area (AREA), specific leaf area (SLA) and surface area to perimeter ratio (ARPE) had the greatest effect on population differentiation (CDA). It is assumed that different environmental conditions affect population differentiation and that high intrapopulation variability is due to intraspecific variability.

Keywords: leaf morphology, pedunculate oak, population, variability

Corresponding author: Branislava Batos, Institute of Forestry, 3 Kneza Višeslava, 11000 Belgrade, Serbia, e-mail: branislavabatos@gmail.com

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp. 543-557

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702543B

Original scientific paper

GENETIC DIVERSITY AND MORPHOLOGICAL VARIABILITY IN GERANIUM PURPUREUM VILL. (GERANIACEAE) OF IRAN

Somayeh Esfandani bozchaloyi¹, Masoud sheidai¹, Maryam keshavarzi²,

Zahra noormohammadi³

¹Faculty Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

²Department of Plant Sciences, Faculty of Biological Science, Alzahra University, Tehran, Iran ³Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

bozchaloyi E. S., M. sheidai, M. keshavarzi, Z. noormohammadi (2017): Genetic diversity and morphological variability in Geranium purpureum Vill. (Geraniaceae) of Iran.- Genetika, vol 49, no2, 543 - 557.

Genetic variability and populations^,structure were studied in 15 geographical populations of Geranium purpureum Vill. (Geraniaceae). Genetic diversity parameters were determined in these populations. AMOVA and Gst analyses revealed the presence of genetic variability within populations and significant molecular difference among the studied populations. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. STRUCTURE and K-Means clustering revealed populations^,genetic stratification. UPGMA dendrogram of populations based on morphological characters was in agreement with NJ tree of molecular data. These results indicated that geographical populations of G. purpureum are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

Keywords: Gene flow, Network, ISSR, Geranium purpureum

Corresponding author: Somayeh Esfandani Bozchaloyi, Faculty Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran; E-mail: somayehesfand@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 559-572

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702559T

Original scientific paper

GENOTYPING FANCONI ANEMIA PATIENTS FROM SERBIA REVEALS

THREE NOVEL FANCD2 VARIANTS

Jelena filipović tričković¹, Vesna mandušić¹, Ivana joksić¹, Dragana vujić²,

Ana valenta šobot¹, Gordana joksić¹

¹Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia

²Mother and Child Health Care Institute of Serbia “Dr. Vukan Čupić”, Belgrade, Serbia

Abstract

tričković filipović J., V. mandušić, I. joksić, D. vujić, A. valenta šobot, G. joksić (2017): Genotyping Fanconi anemia patients from Serbia reveals three novel FANCD2 variants.- Genetika, vol 49, no2, 559 - 572.

Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c.206-246delG; exon 26, c.2396 C>A and intron 28, c.2715+573 C>T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c.206-246 delG and exon 26, c.2396 C>A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.

Keywords: deep intronic variants, Fanconi anemia, FANCD2 variants, splicing mechanism

Corresponding author: Jelena Filipović Tričković, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, 11001 Belgrade, Serbia. Phone: +381 11 3408565; E-mail: filipovicj@vinca.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 573-582

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702573M

Original scientific paper

VARIABILITY OF MORPHOLOGICAL CHARACTERS AMONG ORNAMENTAL SUNFLOWER COLLECTION

Emina mladenović¹, Sandra cvejić², Siniša jocić², Jelena čukanović¹,

Milan jocković², Goran malidža²

¹Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

mladenović E., S. cvejić, S. jocić, J. čukanović, M. jocković, G. malidža (2017): Variability of morphological characters among ornamental sunflower collection.- Genetika, vol 49, no2, 573 - 582.

The research describes the field comparison of 81 decorative sunflower genotypes. In order to assess genetic diversity of sunflower genotypes the studies were conducted in the field conditions during 2010-2015 at the Institute of Field and Crops, Novi Sad, Serbia. The genetic diversity of species Helianthus annuus L. has enabled the breeding work in the direction of the decorating and plant landscaping. Depending on the qualitative and quantitative characteristics, production of decorative sunflowers can be divided into three directions. The first line is for the production of cut flowers, the second one is for garden production and the third line is for the production of pot plants. The direction of production dictates the main breeding objectives, which may include: plant architecture, the color of ray and disc flowers and duration of flowering. Investigation of the genetic variability of ornamental sunflowers relies on quantitative traits of which the greatest variability was observed in branching and plant height, which are also the most important qualities for production. The quantitative characteristics of decorative sunflowers have been examined on the basis of 81samples.

Keywords: collection, Helianthus annuus L., morphological traits, plant description, variability.

Corresponding author: Emina Mladenović, University of Novi Sad, Faculty of Agriculture, Sq. Dositeja Obradovića 8, 21000 Novi Sad, Serbia, Phone: +381 21 485 3269, E-mail: eminam@polj.uns.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp.583-598

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702583P

Original scientific paper

ASSESSMENT OF MOLECULAR AND PHENOTYPIC DIVERSITY AMONG WINTER WHEAT CULTIVARS

Sonja petrović¹, Sonja marić¹,Tihomir čupić², A. rebekić¹, Ivana rukavina³

¹Faculty of Agriculture, University J.J. Strossmayer, Osijek, Croatia

²Institute of Agriculture, Osijek, Croatia

³Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Seeds and Seedlings, Osijek, Croatia

Abstract

petrović S., S. marić,T. čupić, A. rebekić, I. rukavina (2017): Assessment of molecular and phenotypic diversity among winter wheat cultivars - Genetika, vol 49, no. 2, 583-598.

Comparing results of different genetic diversity estimates can be useful in parental selection for plant breeders. Forty winter wheat cultivars, from three Croatian breeding centres and four foreign countries, were used to utilize and compare agronomic, morphologic and molecular based genetic diversity estimates. Ten morphologic descriptors according to UPOV guidelines and eight agronomic traits were used to establish phenotypic data. Molecular data consisted of 26 SSR and four combinations of AFLP markers, covering all three wheat genomes. Agro-morphologic data showed variability especially regarding plant height (CV=18.44%), yield (CV=22.02%), and ear emergence (range=8). Discriminant analysis confirmed grouping among cultivars was mostly influenced by number of days to heading and yield. The four AFLP primer combinations and 26 SSR markers yielded 108 polymorphic bands. The UPGMA based on phenotypic data, arranged cultivars in four clusters, with one distinctive outlier, cultivar U1. The UPGMA based on molecular data also arranged cultivars in four clusters, with one distinctive outlier, cultivar Antonius. The similarities based on all four genetic diversity estimates reflected, on average, the degree of relatedness of cultivars used. No correlations between phenotypic and molecular data were found implying that both types of data should be used for genetic diversity estimates in order to cover wider variability between tested cultivars.

Keywords: wheat; genetic diversity; phenotypic traits; molecular markers

Corresponding author: Sonja Petrović, Faculty of Agriculture in Osijek, University J.J. Strossmayer, Vladimira Preloga 1, Croatia, Telephone: 385-31-554-923, Fax: 385-31-554-853

E-mail: spetrovic@pfos.hr

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 599-611

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702599M

Original scientific paper

INFLUENCE OF GSTT1 AND GSTM1 NULL GENOTYPES ON DIFFERENTIATED THYROID CANCER RISK AND BASELINE AND RADIOIODINE INDUCED CYTOGENETIC DAMAGE IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS

Olivera milošević-djordjević¹^,3, Darko grujičić¹, Marina radović jakovljević¹, Dragoslav marinković², Sladjana dimitrijević¹, Olgica mihaljević⁴, Ljiljana mijatović-teodorović⁴ and Snežana živančević –simonović⁴

¹ University of Kragujevac, Serbia, Faculty of Science, Kragujevac

²Serbian Academy of Sciences and Arts, Belgrade, Serbia

³University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Genetics, Kragujevac

⁴University of Kragujevac, Serbia, Faculty of Medical Sciences, Department of Pathophysiology, Kragujevac

Abstract

milošević-djordjević O., D. grujičić, M. radović jakovljević, D. marinković, S. dimitrijević, O. mihaljević, Lj. mijatović-teodorović and S. živančević –simonović (2017): Influence of GSTT1 and GSTM1 null genotypes on differentiated thyroid cancer risk and baseline and radioiodine induced cytogenetic damage in peripheral blood lymphocytes of patients. - Genetika, vol 49, no. 2, 599 - 611.

As it is known that genetic polymorphisms of glutathione S-transferases (GST) have been associated with a variety of human diseases including cancer, we have analyzed the impact of GSTT1 and GSTM1 null genotypes on the risk of development of differentiated thyroid cancer (DTC) and cytogenetic changes in peripheral blood lymphocytes of DTC patients before and after radioiodine therapy. The polymorphism of GSTT1 and GSTM1 genes were genotyped using multiplex polymerase chain reaction (PCR) and cytokinesis - block micronucleus (MN) assay to assess cytogenetic changes. GSTT1 and GSTM1 null were predominantly found in patients, but statistical significance was observed only for GSTT1 null. The null genotypes increased risk of development of DTC; GSTT1 null a 4.5 times (p < 0.05), GSTM1 null about 3 times but on the border of statistical significance (p = 0.057), while combination of dual null genotypes almost 7 times (p < 0.05) increased risk. No significant effects of the null genotypes as well as their interactions with potential modifiers of MN (diagnose, age, gender and smoking habits) were observed on both baseline and radioiodine-induced values of MN and cytokinesis block proliferation index (CBPI) in DTC patients. Results suggest that both GSTT1 and GSTM1 null genotypes increased risk for DTC but to a greater extent GSTT1 null. Null genotypes of GSTT1 and GSTM1 did not have potential to influence baseline and radioiodine-induced values of MN and CBPI, so that absence of T1 and M1 isoenzymes did not cause increased mutagen sensitivity of PBLs of DTC patients.

Keywords: differentiated thyroid cancer, GSTT1 and GSTM1 null genotypes, micronuclei, lymphocytes, radioiodine

Corresponding author: Olivera Milošević – Djordjević, University of Kragujevac, Faculty of Science, Radoja Domanovića 12, 34 000 Kragujevac, Serbia, E-mail:olivera@kg.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 613-626

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702613S

Original scientific paper

GENETIC STRUCTURE AND DIVERSITY OF EUROPEAN CHESTNUT

(Castanea sativa MILL.) POPULATIONS IN WESTERN BALKANS:

ON A CROSSROAD BETWEEN EAST AND WEST

Azra skender ¹, Mirsadkurtović², Narispojskić³, Belma kalamujić STROIL^3*,

Semina hadžiabulić⁴, Fuad gaši²

¹Biotechnical Faculty, University of Bihać

²Faculty of Agriculture and Food Sciences, University of Sarajevo

³Institute for Genetic Engineering and Biotechnology, Laboratory for Molecular Genetics of Natural Resources, University of Sarajevo

⁴Agromediterranean Faculty, University Džemal Bijedić of Mostar

Abstract

skender A., M.kurtović, N.pojskić, B. kalamujić Stroil, S. hadžiabulić, F. gaši (2017): Genetic structure and diversity of European chestnut (Castanea sativa mill.) populations in Western balkans: on a crossroad between east and west.- Genetika, vol49, no2, 613 - 626.

European chestnut (Castanea sativa Mill.) is highly valued in the western Balkans as a source of timber and fruit, but also as an important source of nectar and pollen for the production of honey. In this study, four chestnut populations, covering a major portion of the western Balkans, and a reference population from the northern Italy were examined using 21 microsatellite markers. The highest genetic diversity was detected within the populations geographically closest to the Italian Peninsula, which also displayed the highest level of admixture with the samples from Italy. The strongest genetic differentiation was noted among the southern and eastern chestnut populations from Bosnia and Herzegovina (B&H) (Gst=12.05%). This pronounced differentiation is probably caused by the genetic adaptations to notably different climatic conditions present in the south (Mediterranean climate) and east (Continental climate) of B&H. The clear genetic differentiation of the southern and eastern B&H chestnut populations from the Italian population, determined by pairwise Gst, FCA and Bayesian Structure analyses, indicates that these populations most likely originated from independent shelter zones (refugia), after the last glaciation period. Based on these results we propose a presence of an introgression zone in the northwestern Balkans, established through gene flow from the Italian and the Balkan Peninsula. The obtained insights into the structure of all analyzed populations will significantly contribute towards establishing a regional conservation and utilization strategy for European chestnut in western Balkans.

Keywords: genetic differentiation, European chestnut, introgression zone, microsatellites, refugia

Corresponding author: Belma Kalamujić Stroil, Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina, Phone:+38733220926, fax:+38733442891,E-mail: belma.kalamujic@ingeb.unsa.ba

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 627-634

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702627P

Original scientific paper

EVALUATION OF DGAT1-EXON 8 K232A SUBSTITUTION IN GIR AND KANKREJ (Bos indicus), INDIAN ORIGIN CATTLE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS

Jainikkumar PATEL and Jenabhai CHAUHAN*

P.G Department of Genetic, Ashok and Rita Patel Institute of Integrated Studies in Biotechnology and Allied Sciences (ARIBAS), Affiliated to Sardar Patel University,

New Vallabh Vidyanagar, India

Abstract

Patel J and J. Chauhan (2017): Evaluation of DGAT1 -exon 8 K232A substitution in gir and kankrej (Bos indicus), Indian origin cattle and its association with milk production traits. - Genetika, vol 49, no.2, 627 - 634.

The aim of the present study was to screen the genotype profile of bovine DGAT1 gene among Gir and Kankrej cattle in Gujarat, India. Total number of 200 Gir cattle and 100 Kankrej cattle were evaluated for CfrI-RFLP based genotyping of DGAT1 gene. We observed that only two genotypes (KK and KA) exist among the studied population with the alleles frequency of 0.925 (n=170) and 0.075 (n=30), respectively for K and A in Gir cattle as well as 0.895 (n=79) and 0.105 (n=21) respectively in Kankrej cattle. The overall calculated allele frequency for K and A was 0.915 and 0.085, respectively in both zebu (Bos indicus) cattle. Association of genotypes with milk production traits revealed that KA had significant (P<0.05) effect on total milk yield, as compared to KK in Gir and Kankrej cattle breed. Similarly, KK genotype had significant (P<0.05) effect on fat percentage, as compared to KA in Gir and Kankrej.

Keywords: DGAT1-exon 8, Polymorphism, Bos indicus, PCR-RFLP, Milk production traits

Corresponding author: Jenabhai Chauhan, P.G. Department of Genetic, Ashok and Rita Patel Institute of Integrated Studies in Biotechnology and Allied Sciences (ARIBAS), Affiliated to Sardar Patel University, New Vallabh Vidyanagar - 388121, India, jbc109@gmail.com, jenabhaichauhan@aribas.edu.in

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 635-646

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1702635M

Original scientific paper

RELATIONSHIP BETWEEN GENETIC DIVERSITY AND COLD-TOLERANCE OF MAIZE INBRED LINES

Marija MILIVOJEVIĆ*, Ana NIKOLIĆ, Ksenija MARKOVIĆ, Milomir FILIPOVIĆ,

Jelena VANČETOVIĆ, Tanja PETROVIĆ, Jelena SRDIĆ

Maize Research Institute “Zemun Polje” Belgrade, Serbia

Abstract

Milivojević M., A. Nikolić, K. Marković, M. Filipović, J. Vančetović, T. Petrović, J. Srdić (2017): Relationship between genetic diversity and cold-tolerance of maize inbred lines.- Genetika, vol 49, no2, 635 - 646.

Early maize sowing enables longer growing season with enhanced possibility of achieving higher and more stable yields, and better chances of avoiding summer droughts. For early sowing, cold-tolerant maize genotypes should be used. Breeding maize, tolerant to low temperatures, requires knowledge of genetic diversity and heterotic patterns of breeding material. The objective of this study was to determine genetic diversity of 15 ZP maize inbred lines applying the method of protein markers (UTLIEF method), and to establish correspondence between thus obtained classification with the results of cold test (CT) and field emergence (FE). During two production seasons (2011 and 2014), 15 maize inbred lines were self-pollinated. Pedigree data showed that material belongs to different maturity and heterotic groups. Cold tolerance was assessed in laboratory (2015) by cold test (7.5 °C, 10 days), and field trials on two locations during two successive years (2015, 2016). ZP maize inbred lines showed very good response to stressful conditions of CT and FE. Inbred lines with Lancaster background were more sensitive to low temperatures than inbred lines with BSSS and Iowa Dent background. Based on UTLIEF method two inbred lines with Lancaster background (ZPL 5 and ZPL 7) were grouped by cluster analysis together with Iowa Dent inbred lines, that also expressed better cold tolerance, and thus exceptional consent was achieved with the results of CT and FE. Classification of maize inbred lines based on UTLIEF method, followed by cluster analysis and PCA, showed good agreement with pedigree data, which points out that this method could be successfully applied for genetic classification of breeding material of a wide genetic background.

Keywords: maize, cold-tolerance, genetic diversity, UTLIEF

Corresponding author: Marija Milivojevic, Maize Research Institute Zemun Polje, Slobodana Bajica 1, 11185 Zemun Polje, Serbia, Phone: +381648406209, Fax: +381113754989; e-mail: mmarija@mrizp.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 647-662

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702647T

Original scientific paper

EVALUATION OF DIVERSITY IN BULGARIAN PEPPER CULTIVARS BY AGRONOMICAL TRAITS AND ISSR MARKERS

Stefan Tsonev¹, Velichka Todorova², Stanislava Groseva²,

Teodora Popova³, Elena G. Todorovska^1*

¹AgroBioInstitute, Sofia, Bulgaria

²Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria

³Department of Developmental Biology, Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria

Abstract

Tsonev S., V. Todorova, S. Groseva, T. Popova, E.G. Todorovska (2017): Evaluation of diversity in Bulgarian pepper cultivars by agronomical traits and ISSR markers.- Genetika, vol 49, no2, 647 - 662.

Information about the genetic variation among cultivars of vegetable crops is of vital importance for improvement of plant breeding programmes worldwide. The objectives of this study were to group 19 pepper (Capsicum annuum L.) cultivars from the collection of Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria into clusters according to their distances as estimated by agronomic traits and 9 di- and tri -nucleotide inter simple sequence repeat polymorphism (ISSR) markers and to assess the relationships between them. The phenotypic characterization during 3 consecutive years revealed significant differences among Bulgarian cultivars for the studied 13 phenotypic traits. The biplot analysis of quantitative traits showed that the most strongly correlating traits with the first axis (55.6% of variance) were fruit width, fruit weight and pericarp thickness (in the negative direction of the axis), and plant height (PH) (in the positive direction). The most discriminative traits, considering the second axis (22.6% of variance) were fruit length (FL) and to a lesser extent the stem height (StH). The correspondence analysis of the qualitative traits showed that the intensity of the colour of the fruit (before and at maturity), fruit colour before maturity and fruit shape in longitudinal section were the most discriminative characteristics for the first two dimensions. The agronomic traits data and 7 dinucleotide ISSR primers were used to estimate the pairwise genetic distances. Higher mean phenotypic distance (0.414) in comparison to the genotypic ones (0.214) among the cultivars was observed, indicating higher phenotypic diversity among them. A highly signiﬁcant, positive correlation between the agronomic data and ISSR marker-based matrices (r=0.41, p=0.001) was detected. This indicates that ISSR distance tended to reﬂect that of the agronomics ones. However, additional molecular studies and large collection of highly diverse genotypes are needed to reveal associations between the marker loci and specific QTLs. The research initiated is a base for more precise estimation of genetic distances between pepper genotypes from the available large collection of landraces, local and modern cultivars including large number of highly polymorphic markers.

Keywords: genetic distances, genetic diversity, ISSR markers, pepper (Capsicum annum L.), phenotypic diversity

Corresponding author: Elena G. Todorovska, AgroBioInstitute, 8 Dragan Tsankov, 1164 Sofia, Bulgaria, Phone: +359 2 9635407, Fax: +359 2 9635408, E-mail: e.g.todorovska@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 663-675

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702663C

Original scientific paper

ASSESSMENT OF SUGAR BEET ROOT YIELD BY AMMI ANALYSIS

Mihajlo ćirić^*1, Živko ćurčić¹, Milan mirosavljević¹, Ana marjanović jeromela¹,Goran jaćimović², Slaven prodanović³, Tomislav živanović³

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

³University of Belgrade, Faculty of Agriculture, Belgrade-Zemun, Serbia

Abstract

ćirić M., Ž. ćurčić, M. mirosavljević, A. marjanović jeromela,G. jaćimović, S. prodanović, živanović (2017): Assessment of sugar beet root yield by AMMI analysi.- Genetika, vol 49, no2, 663 - 675.

Sugar beet cultivars have different responses in various environments, such as different locations, years, mineral nutrition treatments or the combination of these factors, due to genotype × environment interaction. Additive main effect and multiplicative interaction (AMMI) is one of the most commonly used multivariate methods for analysis and visualization of genotype × environment interaction data. The main goals of the present study were to (i) investigate the application of AMMI method in the analysis of genotype × fertilizer interaction in sugar beet, (ii) to assess genotype × fertilizer interaction, and (iii) to identify sugar beet cultivars with the most stable response and high yield performance across different mineral nutrition treatments. The trial with eight sugar beet cultivars was conducted in two successive growing seasons at Rimski šančevi, Serbia. The different levels of nitrogen, phosphorous and potassium fertilizers (0, 50, 100 and 150 kg ha^-1) and their combinations represented specific environments for testing genotype × fertilizer interaction. Results from the analysis of variance indicated that the fertilizer treatment, cultivars, and their interaction significantly affected root yield variation in both seasons. Results from our study suggest that AMMI model with two and three first IPCA axes were recommended in 2014 and 2015, respectively. According to AMMI 1 and AMMI 2 biplot, E14 and E15 were high yielding and among the most stable treatments in both years. Among high yielding genotypes in 2014, G4 and G8 stand out as the most stable, while in the following year G3 had the lowest interaction score. AMMI analysis enabled identification of specific associations between cultivars and different mineral nutrition treatments, which was important for adjustment of fertilizer management for each cultivar in order to achieve high root yield with decreased and more rational fertilizer doses.

Keywords: Beta vulgaris L., G × E interaction, mineral nutrition, multivariate analysis, root yield

Corresponding author: Mihajlo Ćirić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad; Phone: 0214898328; Fax: 021 4898222; E-mail: mihajlo.ciric@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp 677-691

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1702677W

Original scientific paper

EFFECTS OF Glu-1 AND Glu-3 ALLELIC VARIATIONS ON WHEAT GLUTENIN MACROPOLYMER (GMP) CONTENT AS REVEALED BY SIZE-EXCLUSION HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (SE-HPLC)

Jisu WU^1,⁵, Xiaobin LU^1,⁵, Zitong YU^1,⁴^,⁵, Caixia HAN¹, Xiaohui LI¹,

Slaven PRODANOVIC^3*, Yueming YAN^1,2*

¹College of Life Science, Capital Normal University, Beijing, China

²Hubei Collaborative Innovation Center for Grain Industry (HCICGI),

Jingzhou, China

³Faculty of Agriculture University of Belgrade, Belgrade, Serbia

⁴School of Veterinary & Life Sciences, Murdoch University and Australian Export Grains Innovation Centre, Perth, Australia

⁵Contributed equally to this work

Abstract

Wu J., X. Lu, Z. Yu, C. Han, X. Li, S. Prodanovic, Y.Yan (2017): Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC).- Genetika, vol 49, no. 2, 677-691.

Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-D1d encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.

Keywords: bread wheat, HMW-GS, LMW-GS, glutenin macropolymer, SE-HPLC

Corresponding author: Slaven Prodanovic, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia, phone: +381638687246, fax: +381113161987, e-mail: slavenp@agrif.bg.ac.rs

Yueming Yan, College of Life Science, Capital Normal University, Beijing, Xisanhuan Beilu 105, 100048 Beijing, China, phone: +861068902777, fax: +861068902777, e-mail: yanym@cnu.edu.cn

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 693-704

UDC 575

https://doi.org/10.2298/GENSR1702693P

Original scientific paper

MOLECULAR CHARACTERIZATION OF SOME SELECTED PERSIMMON GENOTYPES AND CULTIVARS BY SRAP AND SSR MARKERS

Hasan Pinar¹, Ercan Yildiz², Mustafa Kaplankiran³, Celil Toplu³, Mustafa Unlu⁴,

Sedat Serce⁵, Sezai Ercisli^6*

¹Erciyes University, Department of Horticulture, Kayseri-Turkey

²Usak University, Department of Horticulture, Usak-Turkey

³Mustafa Kemal University, Department of Horticulture, Hatay-Turkey

⁴Alata Horticulture Research Institute, Mersin-Turkey

⁵Omer Halisdemir University, Faculty of Agricultural Sciences and Technologies, Nigde-Turkey

⁶Ataturk University, Faculty of Agriculture, Department of Horticulture, Erzurum-Turkey

Abstract

Pinar H., E. Yildiz, M. Kaplankiran, C. Toplu, M. Unlu, S. Serce, S. Ercisli (2017): Molecular characterization of some selected persimmon genotypes and cultivars by SRAP and SSR markers.- Genetika, vol 49, no. 2, 693 - 704.

In this study, SRAP and SSR markers were employed to determine genetic relationships among 42 persimmon genotypes (Diospyros kaki Thunb) obtained from Hatay province and 3 persimmon cultivars, 2 of which belong to Diospyros kaki Thunb and one belongs to Diospyros oleifera Cheng. Genetic relationships were determined by using a total of 29 molecular DNA primers (SRAP and SSR). Of these primers, 21 SRAP primer combinations produced a total of 107 bands and 77.6% of them were polymorphic; 8 SSR primers produced 26 polymorphic bands with an average polymorphism ratio of 84.6%. The SRAP and SSR markers produced 4.6 bands as average and the number of bands produced per marker was calculated as 3.6. The lowest similarity was observed between MK-113 (Diospyros oleifera Cheng) and the other genotypes all belongs to Diospyros kaki Thunb (with similarity ratios of 0.41-0.69 for SRAP primers, between 0.25-0.67 for SSR primers). The genotypes/cultivars belongs to Diospyros kaki had similarity ratio between 0.98-1.00 according to SRAP and SSR markers. This synonym or similarity could be results of clonal propagation rather than autogamy.

Key words: Persimmon, selection, genetic diversity, molecular markers

Corresponding author: Hasan Pinar, Erciyes University Agricultural Faculty Department of Horticulture 38300 Kayseri-Turkey, Phone: +903524371790, Fax:+903524376209, e-mail hpinarka@yahoo.com; Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: +90 4422312599, Fax: +90 4422360958, e-mail: sercisli@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 705-715

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702705H

Original scientific paper

GENETIC AND MULTIVARIATE PHENOTYPIC ANALYSES OF SOME SELECTION INDICES IN PISTACHIO (Pistacia vera L.) CULTIVARS UNDER DROUGHT STRESS CONDITIONS

HOJJAT HASHEMINASAB¹* and MOHAMMAD TAGHI ASSAD²

¹Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran

²Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran

Abstract

Hasheminasab H. and M.T. Assad (2017): Genetic and multivariate phenotypic analyses of some selection indices in pistachio (Pistacia vera L.) cultivars under drought stress conditions.- Genetika, vol 49, no.2, 705 - 715.

Heritability and Genetic Gain are commonly used by plant breeders to estimate the accuracy of a selection index and used for measuring the response to selection. The aim of the present study was the genetic and phenotypic analyses of some physiological traits to identify the most reliable of them for selective breeding of pistachio cultivars under drought stress condition. Nineteen Pistachio (Pistacia vera L.) cultivars with wide range of tolerance to drought stress were collected from across the orchards of Rafsanjan (Iran’s center of pistachio cultivation) and were used in randomized complete block design with three replications under two environmental conditions (normal and water stress) in 2011-2012. The results of genetic analysis showed that high magnitude of phenotypic and genotypic coefficient of variation along with broad sense heritability and genetic gain were estimated in relative water protection (RWP), excised leaf water loss (ELWL), relative water content (RWC) and relative water loss (RWL) under drought stress condition, indicating that the inheritance of these traits can be mainly controlled by additive gene effects followed by reflecting the possibility of effective selection for genetic improvement of these traits. Path analysis revealed that RWP had the highest direct and RWL and WRC indirect effects on YSI. Principal component analysis indicated that selected indices were reliable for classification and identification of drought-tolerant pistachio cultivars and identified RWP, RWC and WRC as the best indicators for screening drought tolerant genotypes.

Key words: Pistachio, drought stress, genetic analysis, multivariate phenotypic analysis.

Corresponding author: Hojjat Hasheminasab, Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran 7738153339, Tel: +98 34225201, Mob: +98 9139946843, E-mail: hojathashemi@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 2 (2017), pp. 717-728

Serbia

UDC 575

https://doi.org/10.2298/GENSR170271A

Original scientific paper

TRANSFER OF HUMAN PROINSULIN GENE INTO CUCUMBER

(Cucumis sativus L.) VIA AGROBACTERIUM METHOD

Kameh ABOOKAZEMI^1*, Mokhtar JALALI JAVARAN¹, Mehdi MOHEBODINI²,

and Akbar VASEGHI³

¹Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

²Department. of Horticulture Science, University of Mohaghegh Ardabili

³Young Researchers and Elite Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran

Abstract

Abookazemi K., M. Jalali Javaran, M. Mohebodini and A. Vaseghi (2017): Transfer of human proinsulin gene into cucumber (Cucumis sativus L.) via agrobacterium method- Genetika, vol 49, no.2, 717 - 728.

Nowadays, approximately 5.8% in adult population around the world are suffering by diabetes. It can be caused by an increase in risk factors such as being overweight. Also it has been estimated that the number of patients will be doubled in near future and the demands for insulin hormone will be growing up by 3 to 4 % annually. Therefore, it’s necessary to develop new methods for hormone production with high rate of capacity in future. By advanced technology of transgenic DNA, the transgenic plants are introduced as an attractive system for expression and production of many kinds of pharmaceutical proteins. In this study, we investigated transfer of Human Proinsulin Gene into the Cucumber (Cucumissativus L.). Transgenic cucumber could be a great prospect for future source of eatable insulin pharmaceutical drugs to be taken by patients.Agrobacterium tumefaciensstrain LBA4404 carrying proinsulin genes with CaMV 35S promoter was used for the transformation purpose. The transgenic plants were analyzed by PCR, RT-PCR, SDS-PAGE, Dot blot and Electrochemiluminescence techniques. Production of proinsulin in cucumber could be a great prospect in molecular farming of human proinsulin.

Keywords: Gene expression analysis, Human proinsulin transformation, Recombinant pharmaceutical protein, transgenic cucumber

Corresponding author: Kameh Abookazemi, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran, Tel:+989121436092, E-mail: kamehab@yahoo.com

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Published in „GENETIKA“ Vol. 49 No. 2 (2017), pp. 729-742

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1702729P

Original scientific paper

MODE OF INHERITANCE and AMMI analysis of onion (Allium cepa L.)

bulb traits

Nenad Pavlović¹, Zdenka Girek¹, Milan Zdravković¹, Jelica Gvozdanović Varga², Đorđe Moravčević³, Jasmina Zdravković¹

¹Institute for vegetable crops, Smederevska Palanka, Serbia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Faculty of Agriculture, Beograd-Zemun, Serbia

Abstract

Pavlović N., Z. Girek, M. Zdravković, J. Gvozdanović Varga, Đ. Moravčević, J. Zdravković (2017): mode of inheritance and AMMI analysis of onion (Allium cepa L.) bulb traits.-Genetika, vol 49, no.2, 729 - 742.

The success in growing agricultural plants depends on genotype grown, environment and growing technology applied. The impacts of these factors should not be studied separately. Interaction among genotypes and environment was crucial for breeding work and improvement of variety characteristics. Very often in production practice it happens that when comparing the same varieties in different soil and weather conditions they are ranked differently. One of the key aims set at the breeders is breeding for the traits of wide adaptability and calculating of the complex components of genetic variance which is the main condition for successful breeding program such as breeding for increased yield and yield components. Trial was set up at random block system in five repetitions. Ten onion genotypes from the Institute for Vegetable Crops germplasm were used in this trial. After choosing the most stabile genotypes, they were crossed by applying full diallel without reciprocals. Then, a field trial was set up with parents and hybrids of F₁ and F₂ generation. The experiment has been set up at the trial field of the Institute for Vegetable Crops in Smederevska Palanka, for three years. The best adaptability in the experiment, for mass of the onion bulb had genotypes Makoi bronzi and Holandski žuti, while for average yield, it was Jasenički crveni. Also, components of genetic variance in both progeny generations were calculated for mass, yield and height of fresh bulb. The most common heredity mode for mass and yield of the bulb was super-domination and domination of a better parent.

Key words: interaction G x E, heredity mode, onion

Corresponding author: Nenad Pavlović, Institute for vegetable crops, Karadjordjeva 71, 11420 Smederevska Palanka, npavlovicpb@gmail.com

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp. 743-753

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702743O

Original scientific paper

REGRESSION TREE ANALYSIS FOR PREDICTING BODY WEIGHT OF NIGERIAN MUSCOVY DUCK (Cairina moschata)

Abel Olusegun OGUNTUNJI

Department of Animal Science and Fisheries Management,

Bowen University, Iwo, Osun State, Nigeria

Abstract

Oguntunji A. O. (2017): Regression tree analysis for predicting body weight of Nigerian muscovy duck (Cairina moschata).- Genetika, vol 49, no.2, 743- 753.

Morphometric parameters and their indices are central to the understanding of the type and function of livestock. The present study was conducted to predict body weight of adult Nigerian Muscovy ducks from 9 morphometric parameters and 7 body indices and also to identify the most important predictor of BWT among them using regression tree analysis (RTA). The experimental birds comprised of 1,020 adult Nigerian Muscovy ducks randomly sampled in Rain Forest (203), Guinea Savanna (298) and Derived Savanna (519) agro-ecological zones. Result of RTA revealed that compactness; body girth and massiveness were the most important independent variables in predicting BWT and were used in constructing RT. However, compactness was the best of the three predictors and gave the best estimate of BWT. The combined effect of the three predictors was very high and explained 91.11% of the observed variation of the target variable (BWT). The optimal regression tree suggested that Muscovy ducks with compactness >5.765 would be fleshy and have highest BWT. The result of the present study could be exploited by animal breeders and breeding companies in selection and improvement of BWT of Muscovy ducks.

Keywords: Agro-ecological zones, body indices, compactness, morphometric parameters, Muscovy ducks

Corresponding author: Abel Olusegun Oguntunji, Department of Animal Science and Fisheries Management,Bowen University, Iwo, Osun State, Nigeria, Phone no: +2348139439458, abelmendel@yahoo.co.in

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Published in „GENETIKA“ Vol. 49, No.2 (2017), pp.755-764

Serbia

UDC 575

https://doi.org/10.2298/GENSR1702755C

Original scientific paper

AN OVERVIEW OF MOLECULAR GENETIC LINKAGE MAPS IN Lilium SPP.

Li-jing CHEN¹^#*, Fu-xing CHENG¹^#, Shao-kun SUN¹, Jun SUN², Muhammad IRFAN³,

Li ZHANG¹*

¹ Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology，Shenyang Agricultural University, Shenyang, Liaoning, China

² Cultivation Department, Liaoning Institute of Economic Forestry, Dalian, China

³ Department of Biotechnology, University of Sargodha, Sargodha Pakistan

Abstract

Chen L.-J., F.-x. Cheng, S.-k. Sun, J. Sun, M. Irfan, L. Zhang (2017): An overview of molecular genetic linkage maps in Lilium spp..- Genetika, vol 49, no. 2, 755 - 764.

Molecular genetic linkage maps are powerful tools used to identify quantitative trait loci and facilitate molecular marker-assisted breeding. A review of the molecular markers applied and genetic linkage maps constructed for Lilium was conducted. High-density linkage maps constructed for other plant species were also analyzed. Problems related to the construction of molecular genetic linkage maps for Lilium were explored. High-density linkage maps for Lilium may be developed on the basis of the construction strategies of several detailed linkage maps.

Keywords: Molecular marker, genetic linkage map, quantitative trait locus (QTL), high-density map, Lilium

Corresponding author: Li-jing Chen, E-mail: chenlijing1997@126.com, chenlijing905@yahoo.com. Phone: +86-24-88487164, Fax: +86-24-88492799, Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology，Shenyang Agricultural University, Shenyang, Liaoning 110866, China

Li Zhang，E-mail: zhangli_1997@yahoo.cn, Phone: +86-24-88487164, Fax: +86-24-88492799

^#Joint first author（These authors contributed equally to this work）

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