GENETIKA, Vol

GENETIKA, Vol. 49, No. 3(2017)

Vesna DRAGIČEVIĆ, Milena SIMIĆ, Katarina JOVANOVIĆ RADOVANOV, Milan BRANKOV, Jelena SRDIĆ

REACTION OF SUSCEPTIBLE MAIZE INBRED LINES TO HERBICIDES [Abstract] [Full text]

Hejraneh azizi, Masoud sheidai, Valiollah mozaffarian, Zahra noormohammadi

MOLECULAR STUDIES (ISSR, cpDNA) IN Tragopogon buphthalmoides (Asteraceae): POPULATION GENETICS AND TAXONOMIC IMPLICATION [Abstract] [Full text]

Milica fotirić akšić, Radosav cerović, Vera rakonjac, Ivana bakić, Slavica čolić, Mekjell meland

VITALITY AND IN VITRO POLLEN GERMINATION OF DIFFERENT ‘OBLAČINSKA’ SOUR CHERRY CLONES [Abstract] [Full text]

Svetlana vojvodić, Dušica ademović-sazdanić

IMPACT OF HLA -B*27 SUBTYPES WITH INCREASED SUSCEPTIBILITY TO SERONEGATIVE SPONDYLOARTHROPATHIES IN VOJVODINA POPULATION [Abstract] [Full text] [Corregdum]

Saeid yarahmadi, Mohammad Mehdi sohani, Ali Akbar ebadi, Masumeh kheirgoo

TAGGING OF FOUR Rf GENES WITH SELECTIVE GENOTYPING ANALYSIS IN RICE (Oryza sativa L.) [Abstract] [Full text]

Petar zhelev, Ivan evtimov

Diameter growth and survival of local half-sib families of Scots Pine (Pinus sylvestris L.) in Yundola, Bulgaria [Abstract] [Full text]

Ivana RUKAVINA, Sonja PETROVIĆ, Tihomir ČUPIĆ, Sonja VILA, Sunčica GUBERAC and Luka DRENJANČEVIĆ

GENETIC VARIABILITY OF WHEAT GERMPLASM REPRESENTED IN THE SOUTH PANNONIAN REGION [Abstract] [Full text]

Behzad SADEGHZADEH, Seyed Hossein JAMALI, Fatemeh VAFADAR-SHAMASBI

IDENTIFICATION OF MFLP FINGERPRINT FOR HIGHER SEED ZINC ACCUMULATION IN BARLEY DH POPULATION [Abstract] [Full text]

Vojka babić, Violeta andjelković, Ana nikolić, Marija milivojević, Jelena srdić, Aleksandar popović, Natalija kravić

disruption of genetic IDENTITY for genebank maize accessions during conservation [Abstract] [Full text]

Abdulmojeed yakubu, Adebowale E. salako, Marcos de donato, Michael I. takeet, Sunday O. peters, Moses okpeku, Mathew wheto, Ikhide G. imumorin

NUCLEOTIDE SEQUENCE VARIABILITY ANALYSIS OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II DQA1 GENE IN NIGERIAN GOATS [Abstract] [Full text]

Mirjana šijačić-nikolić, Marina nonić, Vedrana lalović, Jelena milovanović, Jelena nedeljković, Dragan nonić

Conservation of Forest Genetic Resources: Key Stakeholders' Attitudes in forestry and nature protection [Abstract] [Full text]

Josip KOVAČEVIĆ, Maja MAZUR, Georg DREZNER, Alojzije LALIĆ, Aleksandra SUDARIĆ, Krešimir DVOJKOVIĆ, Marija VILJEVAC-VULETIĆ, Marko JOSIPOVIĆ, Ana JOSIPOVIĆ, Antonela MARKULJ-KULUNDŽIĆ, Hrvoje LEPEDUŠ

PHOTOSYNTHETIC EFFICIENCY PARAMETERS AS INDICATORS OF AGRONOMIC TRAITS OF WINTER WHEAT CULTIVARS IN DIFFERENT SOIL WATER CONDITIONS [Abstract] [Full text]

Amin moosavi, Mohammad FORAT YAZDI, Masoud DEHGHAN TEZERJANI, Mohammad Hasan SHEIKHHA, Seyed Mahdi HOSEINI, Fatemeh MOEININIA, Mahnaz ZOHAL, Ali MOUSAVI

GLUTATHIONE S-TRANSFERASE M1 - T1 NULL GENOTYPES AND SUSCEPTIBILITY TO HODGKIN’S LYMPHOMA [Abstract] [Full text]

Dragana jošić, Marija stojanoviC, Zorica lepšanović, Vera katić

MOLECULAR CHARACTERIZATION OF Cronobacter sakazakii ISOLATED FROM DIFFERENT HERBAL TEAS AND MIXTURES IN SERBIA [Abstract] [Full text]

Mehmet Kadri bozokalfa, Tansel kaygisiz aşçioğul, Dursun eşiyok

GENETIC DIVERSITY OF FARMER-PREFERRED COWPEA (Vigna unguiculata L. Walp) LANDRACES IN TURKEY AND EVALUATION OF THEIR RELATIONSHIPS BASED ON AGROMORPHOLOGICAL TRAITS [Abstract] [Full text]

Hacer konakli, Zafer konakli, Senol dogan, Damir marjanovic, Serkan dogan

ACTN3 POLYMORPHISM PREVALENCE IN GENERAL POPULATION OF SIX BALKAN COUNTRIES [Abstract] [Full text]

Ahad yousefi, Abdolreza salehi, Mehdi aminafshar, Mohammad bagher sayyadnejad

THE UTERINE MILK PROTEIN (UTMP) GENE IS ASSOCIATED WITH MILK PRODUCTION TRAITS IN IRANIAN HOLSTEIN BULLS [Abstract] [Full text]

Vladan popović, Aleksandar lučić, Ljubinko rakonjac, Branislav cvjetković, Snežana mladenović drinić, Danijela ristić

ASSESSMENT OF GENETIC DIVERSITY OF SILVER FIR (Abies alba Mill.) IN SERBIA USING SSR MARKERS [Abstract] [Full text]

Slavomíra senkovÁ, Jana ŽiarovskÁ, eloy FernÁndez, Danka boŠeĽovÁ, Milan beŽo

IRAP variability in Prunus domestica (L.) Borkh based on Cassandra retrotransposon polymorphism [Abstract] [Full text]

Alireza HAGHIGHI HASANALIDEH, Ezatollah FARSHADFAR, Mehrzad ALLAHGHOLIPOUR

GENETIC PARAMETERS AND COMBINING ABILITY OF SOME IMPORTANT TRAITS IN RICE (Oryza sativa L.) [Abstract] [Full text]

Nada hladni, Milan jocković, Siniša jocić, Vladimir miklič, Dragana miladinović, Miroslav zorić

DIRECT AND INDIRECT EFFECTS OF MORPHOPHYSIOLOGICAL TRAITS ON KERNEL PROTEIN CONTENT OF CONFECTIONARY SUNFLOWER [Abstract] [Full text]

Andrija BRKIĆ, Ivan BRKIĆ, Antun JAMBROVIĆ, Marija IVEZIĆ, Emilija RASPUDIĆ, Mirjana BRMEŽ, Zvonimir ZDUNIĆ, Tatjana LEDENČAN, Josip BRKIĆ, Monika MARKOVIĆ, Goran KRIZMANIĆ, Domagoj ŠIMIĆ

MAIZE GERMPLASM OF EASTERN CROATIA WITH NATIVE RESISTANCE TO WESTERN CORN ROOTWORM (Diabrotica virgifera virgifera LeConte) [Abstract] [Full text]

Sonja MILAŠINOVIĆ, Suzana CVJETIĆANIN, Radivoj BRDAR, Dejan NIKOLIĆ

MORPHOGENETIC VARIABILITY AND GENETIC LOADS AMONG PATIENTS WITH DIFFERENT EXPRESSION OF DEVELOPMENTAL HIP DYSPLASIA [Abstract] [Full text]

Katerina BANDJO ORESHKOVIKJ, Rade RUSEVSKI, Biljana KUZMANOVSKA, Mirjana JANKULOVSKA, Zoran T. POPOVSKI

MOLECULAR DETECTION AND IDENTIFICATION OF ALFALFA MOSAIC VIRUS (AMV) ON PEPPER CULTIVATED IN OPEN FIELDS IN R. MACEDONIA [Abstract] [Full text]

Bahman khalili, Payam ghasemi-dehkordi, Gholamreza pourshahbazi, Hossein yousofi-darani, Morteza hashemzadeh-chaleshtori, Abbas doosti

GENOTYPING OF Trichomonas vaginalis ISOLATES FROM WOMEN IN SHAHREKORD CITY (SOUTHWESTERN IRAN) [Abstract] [Full text]

Abdul QADIR, Niaz ALI, Sohail Ahmad JAN, Malik Ashiq RABBANI, Muhammad Ishaq IBRAHIM,Ashtar KHAN and Haris KHURSHID

ASSESSMENT OF GENETIC VARIABILITY IN Trigonella Foenum-graecum L. GERMPLASM BY SDS-PAGE ANALYSIS [Abstract] [Full text]

Lingbo Zhao, Li Zhang, Jipeng Qu, Yan Yu, Lu Lu, Zhengsong Peng, Wuyun Yang, Shuhong Wei, Jun Yang, Zaijun Yang

Novel Fluorescent Sequence-related Amplified Polymorphism (FSRAP) Markers for the Construction of a Genetic Linkage Map of Wheat (Triticum aestivum L.) [Abstract] [Full text]

Vladanka STUPAR Aleksandar PAUNOVIĆ, Milomirka MADIĆ, Desimir KNEŽEVIĆ

INFLUENCE OF GENOTYPE AND NITROGEN NUTRITION ON GRAIN SIZE VARIABILITY IN SPRING MALTING BARLEY [Abstract] [Full text]

Oreynab Looch MALEKI, Ali HASHEMI, Ghorban Elyasi ZARRINGHABAIE, Mohammad FARHADIAN

ASSOCIATIONS OF POLYMORPHISMS IN THE PROLACTIN RECEPTOR GENE WITH GROWTH TRAIT IN JAPANESE QUAIL (Coturnix coturnix japonica) [Abstract] [Full text]

Iqra REHMAN, Beenish AFTAB, Muhammad BILAL SARWAR, Bushra RASHID, Qurban ALI, Muhammad Umair Majid, Sameera HASSAN, Muhammad AZAM ALI, Idrees AHMAD NASIR, Muhammad SALEEM HAIDER and Tayyab HUSNAIN

GENE EXPRESSION IN RESPONSE TO COTTON LEAF CURL VIRUS INFECTION IN Gossypium hirsutum UNDER VARIABLE ENVIRONMENTAL CONDITIONS [Abstract] [Full text]

Corrigendum

Surma M., et al (2017): A multivariate approach to the selection of pea (Pisum sativum L.) lines obtained by the single seed descent technique.[Abstract] [Full text]

Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 765-774

UDC 575.633.15
https://doi.org/10.2298/GENSR1703765D
Orginal scientific paper

REACTION OF SUSCEPTIBLE MAIZE INBRED LINES TO HERBICIDES

Vesna DRAGIČEVIĆ^1*, MilenaSIMIĆ¹, Katarina JOVANOVIĆ RADOVANOV², Milan BRANKOV¹, Jelena SRDIĆ¹

¹ Maize Research Institute “ZemunPolje”, Belgrade, Serbia

²University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

Abstract

Dragičević V., M. Simić, K. Jovanović Radovanov, M. Brankov, J. Srdić (2017): Reaction of susceptible maize inbred lines to herbicides.- Genetika, Vol 49, No.3, 765-774.

Maize production is inconceivable without herbicide application, and certainly depends on crop susceptibility. Some injuries could be induced by herbicides, what could result in yield losses. This is especially prominent in maize seed production, due to the lines susceptibility to various stressful conditions, including herbicides. Crop response to herbicide application could include whole range of different biochemical reactions such as alterations in content of various metabolites and antioxidants. The experiment was conducted to examine the response of three sensitive maize lines (sugary, popcorn and white kernel maize) to herbicides from sulfonylurea and triketone groups, during the period after herbicide application, when visual injuries are the most obvious and in correlation with grain yield. Variations in soluble proteins, phytic and inorganic phosphorus content, as important metabolites, were followed. The variations in soluble proteins and particularly phytic and inorganic phosphorus content are linked to the expression of susceptibility to herbicides in examined maize lines. Growing season had significant influence on susceptibility. In 2015, as unfavourable season, line ZPT165b expressed the highest susceptibility, having the highest values of examined metabolites at the beginning of experiment. All applied herbicides increased grain yield in 2014, but in 2015 nicosulfuron expressed the lowest selectivity, by decreasing grain yield and soluble proteins up to the 21^th day after herbicide application, when compared to control.

Key words: susceptible maize lines, herbicide stress, phyticphosphorus, inorganic phosphorus, soluble proteins.

Corresponding author: Vesna Dragićević, Maize Research Institute “ZemunPolje”, Slobodana Bajića 1, 11185 ZemunPolje, Serbia, Phone +381113756704, Fax: +381113756707,e-mail: vdragicevic@mrizp.rs

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp.775-789

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703775A
Original scientific paper

MOLECULAR STUDIES (ISSR, cpDNA) IN Tragopogon buphthalmoides (Asteraceae):

POPULATION GENETICS AND TAXONOMIC IMPLICATION

Hejraneh azizi ¹*, Masoud sheidai ¹, Valiollah mozaffarian ²,

Zahra noormohammadi³

¹Faculty of life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

²Research Institute of Forests and Rangelands, Tehran, Iran

³Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University (SRBIAU), Tehran, Iran

Abstract

azizi H., M. sheidai, V. mozaffarian, Z. noormohammadi (2017): Molecular studies (ISSR, cpDNA) in Tragopogon buphthalmoides (Asteraceae): Population genetics and taxonomic implication. - Genetika, Vol 49, No.3, 775-789.

The genus Tragopogon L. with about 110-150 species is distributed in Asia and Europe, while it is represented by 26 species in Iran. Hybridization and polyploidy occurs in the genus Tragopogon that bring about extensive morphological variation in its species. Tragopogon buphthalmoides (Asteraceae) is a perennial herb with common Persian name "Sheng e Iran" and "Sheng e cheshmgaavi" and is used as traditional medicine. Two varieties have been cited for this species in the country, but due to extensive morphological variability, it is difficult to delimit the two varieties. Therefore, the present study was performed to delimit these varieties by using both morphological and molecular markers (cpDNA and ISSRs). We also aimed to provide data on population genetics of the studied species due to its medicinal importance. In total 134 plant specimens of T. buphthalmoides were randomly collected from 16 geographical populations in 7 provinces. The studied plants were considered as two different varieties based on morphological criteria and were subjected to numerical and phylogenetic analyses. PCOA plot of morphological data, identified some plants with intermediate characters. Moreover, MDS plot of ISSR data and ML tree of cpDNA sequences, did not separate plants of the two varieties and the plants were placed intermixed. Therefore, we consider T. buphthalmoides as a single species with extensive morphological variability and it is complex species. Population genetic study identified four genetic groups in the country, while, STRUCTURE analysis based on admixture model as well as population assignment test revealed some degree of gene flow in the studied populations.

Key words: Genetic diversity, STRUCTURE analysis, Taxonomic delimitation, Tragopogon buphthalmoides.

Corresponding author: Hejraneh Azizi, Faculty of life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, Tel:+9809365622133, E-mail: hejraneh.azizi@yahoo.com, azizi@sbu.ac.ir

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp.791-800

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1703791A

Original scientific paper

VITALITY AND IN VITRO POLLEN GERMINATION OF DIFFERENT ‘OBLAČINSKA’ SOUR CHERRY CLONES

Milica fotirić akšić¹, Radosav cerović², Vera rakonjac¹, Ivana bakić³,

Slavica čolić³, Mekjell meland⁴

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²University of Belgrade, Innovation Centre of the Faculty of Technology and Metallurgy, Belgrade, Serbia

³Institute for Science Application in Agriculture, Belgrade, Serbia

⁴ Norwegian Institute of Bioeconomy Research – NIBIO, Ullensvang, Norway

Abstract

akšić fotirić M., R. cerović, V. rakonjac, I. bakić, S. čolić, M. meland (2017): Vitality and in vitro pollen germination of different “Oblačinska” sour cherry clones.- Genetika, Vol 49, No.3, 791- 800.

Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblačinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two ‘Oblačinska’ sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23°C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 μm (clone XIII/1, after 1 h) to >1,100 μm (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 μm min^-1) after 6 h of germination, but rather decreasing until 24 h of germination (0.56-0.83 μm min^-1). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).

Key words: Prunus cerasus L., genotype, acetocarmine, germination medium, pollen tube growth, pollen growth rate.

Corresponding author: Vera Rakonjac, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia

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Published in „GENETIKA“ Vol. 49, No3 (2017), pp. 801-808

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703801V

Original scientific paper

IMPACT OF HLA -B*27 SUBTYPES WITH INCREASED SUSCEPTIBILITY TO SERONEGATIVE SPONDYLOARTHROPATHIES IN VOJVODINA POPULATION

Svetlana vojvodić, Dušica ademović-sazdanić

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, University of Novi Sad, Medical faculty Novi Sad

Abstract

vojvodić S., D. ademović-sazdanić (2017): Impact of Hla-B*27 subtypes with increased susceptibility to seronegative spondyloarthropathies in Vojvodina population.- Genetika, Vol 49, No.3, 801- 808.

The aim of this study is to investigate which subtype of HLA-B*27 was associated with susceptibility of seronegative spondyloarthropathies (SpA) in Vojvodina patients. The dataset of the current study is composed of 172 SpA patients, among which there were 72 HLA-B*27+ (positive) and 100 HLA-B*27Ø(negative) patients. HLA-B*27 allele genotyping was carried out by Polymerase Chain Reaction-Sequence Specific Primer (PCR-SSP) method and low and high resolution tests, respectively. The association of HLA-B*27 subtypes with SpA was determined by the calculation of Relative Risk (RR) and Etiological Fraction (EF), while the allelic distribution between two group of patients was assessed by chi-square test.

In HLA-B*27+ patients with SpA, the most common were patients with Reactive Arthritis (ReA) (41/72 or 56,94%) and Ankylosing Spondylitis (AS) (17/72 or 23,61%). Statistically significant difference was found in HLA-B*27+ and HLA-B*27Øpatients among SpA subgroups studied: Arthritis Psoriatica (PsA), Ankylosing Spondylitis (AS), Undifferentiated Spondyloarthritis (uSpA), Inflamatory Bowel Disease (IBD), Juvenilis Idiopathic Arthritis (JIA) and Reactive Arthritis (ReA). Two HLA-B*27 allele subtypes were found in SpA patients in Vojvodina: B*27:02 and B*27:05. Both HLA-B*27:02 and HLA-B*27:05 alleles have shown positive association with Ankylosing Spondylitis (RR=9.458 for B*27:02 and RR=6.585 for B*27:05), but significant Etiological Fraction (EF) or population attributive risk with value of 0.281 was found only for HLA-B*27:05 subtype. In this study we have showed, for the first time in Vojvodina population, that HLA-B*27:05 subtype have strong positive or susceptible association with Ankylosing Spondylitis.

Key words: seronegative spondyloarthropathies, HLA-B*27 subtypes

Corresponding author: Svetlana Vojvodić, M.D., Ph.D., Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Compartment, Hajduk Veljkova 9a, 21000 Novi Sad, Serbia; Tel.: +381-21-4877-963; Fax: +381-21-4877-978; E-mail: svetlana.vojvodic021@gmail.com

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp. 809-818

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703809Y
Original scientific paper

TAGGING OF FOUR Rf GENES WITH SELECTIVE GENOTYPING ANALYSIS

IN RICE (Oryza sativa L.)

Saeid yarahmadi^1,5*, Mohammad Mehdi sohani², Ali Akbar ebadi³,

Masumeh kheirgoo⁴

¹Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences,

University of Guilan, Rasht, Iran

²Biotechnology Department, Faculty of Agriculture Science, University of Guilan, Rasht, Iran

³Rice Research Institute of Iran (RRII), Rasht, Iran.

⁴Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

⁵Emam Khomeini Agricultural High School, Aliabad-e Katul, Iran

Abstract

yarahmadi S., M. M. sohani, A. A. ebadi, M. kheirgoo (2017): Tagging of four Rf genes with selective genotyping analysis in rice (Oryza sativa L.).- Genetika, Vol 49, No.3, 809 - 818.

Wild abortive type of cytoplasmic male sterility (WA-CMS) is commercially used for hybrid rice seed production. The linked markers can be used for selection of plants with desirable traits. Tagging of Rf genes was carried out using recessive and dominant class analysis in a large F₂ population from the cross IR58025A×IR42686R. Pollen fertility and seed setting were evaluated at the flowering and maturity stages, respectively. Forty-seven highly sterile and 23 fertile homozygous plants were selected from F₂ population for molecular marker assay. Four Rf genes identified in a good restorer line with high-quality derived from a random mating composite population at the International Rice Research Institute (IRRI). The genetic distance from Rf3 locus with flanking markers RM443 and RM315 on chromosome 1 was 3.7 and 21.2 cM, respectively. RM258, RM591, RM271 and RM6737 on the long arm of chromosome 10 were linked with the Rf6gene with distance of 7.4, 22.6, 6 and 2.9 cM, respectively. Rf₆ was flanked by RM6737 and RM591. The Rf4 gene located on chromosome 7 was linked with RM6344 at a genetic distance of 10.6 cM. RM519 and RM7003 were linked with other Rf gene on chromosome 12 at a genetic distance of 8.5 and 20.8 cM, respectively. Closely linked markers identified in this study could be used for marker assisted selection in a hybrid rice breeding program. A new Rf locus on chromosome 12 that designated Rf7 was linked with RM7003 and RM519.

Key words: fertility restoration, hybrid rice, molecular mapping, recessive class analysis, SSR marker

Corresponding author: Saeid Yarahmadi, Department of Agronomy and Plant Breeding, Faculty of Agricultural sciences, University of Guilan, Rasht, Iran, Telephone no.: +98-916-3999004, Email: yarahmadi61@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 819-829

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1703819Z
Original scientific paper

Diameter growth and survival of local half-sib families

of Scots Pine (Pinus sylvestris L.) in Yundola, Bulgaria

Petar zhelev, Ivan evtimov

University of Forestry, Sofia, Bulgaria

Abstract

zhelev P., I. evtimov (2017): Diameter growth and survival of local half-sib families of scots pine (Pinus sylvestris L.) in Yundola, Bulgaria.- Genetika, Vol 49, No.3, 819 - 829.

The paper presents results of a study on a progeny test in Yundola established in 1966 and comprising 26 local plus trees of Scots Pine. Diameters at breast height (DBH) were measured in 1996 and 2012. There were substantial differences among the performance of half-sib families regarding the diameter growth. The best performing families 30 and 46 years after planting were the same, but there were statistically significant changes in the rank of the families as a whole. Mean survival rate was 91% at the age of 30 and 79% at the age of 46, which is relatively high. Individual heritability increased from 0.2 to 0.5-0.6 from the age 30 to 46 and additive genetic coefficient of variation was 5.2% at the age of 30 and 10.1% at the age of 46. Heritabilities and estimated response to selection were within the range of the results reported in other studies on Scots Pine. The main inferences of the study are that individual selection could be a promising tool for genetic improvement of Scots Pine in the region, and genetic parameters could change over time even after 30 years of age.

Key words: heritability, genetic gain, progeny test, tree breeding

Corresponding author: Petar Zhelev, University of Forestry, 10 Kliment Ohridsky Blvd., 1797 Sofia, Bulgaria, e-mail: zhelev@ltu.bg, phone: +359-2-91907-389, fax: +359-2-8622830.

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp.831-842

Serbia

UDC 575.633.11
https://doi.org/10.2298/GENSR1703831R

Original scientific paper

GENETIC VARIABILITY OF WHEAT GERMPLASM REPRESENTED

IN THE SOUTH PANNONIAN REGION

Ivana RUKAVINA¹, Sonja PETROVIĆ², Tihomir ČUPIĆ³, Sonja VILA², Sunčica GUBERAC²

and Luka DRENJANČEVIĆ¹

¹Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Seeds and Seedlings, Osijek, Croatia

² University of J.J.Strossmayer Osijek, Faculty of Agriculture, Osijek, Croatia

³Agricultural Institute Osijek, Croatia

Abstract

Rukavina I., S. Petrović, T. Čupić, S. Vila, S. Guberac and L. Drenjančević (2017): Genetic variability of wheat germplasm represented in the south Pannonian region.- Genetika, Vol 49, No.3, 831-842.

In this study, genetic variability was investigated among 50 winter wheat varieties (Triticum aestivum L.) which are grown in parts of Croatia, Hungary, Serbia and Slovenia according to 22 morphological characteristics used for DUS (distinctness, uniformity and stability) testing. The average Dice similarity coefficient was 0.371. The determined similarity coefficient was in range 0.083 – 0.776. A significant variability of 6.21% in the breeding programs according to period was determined as well as significant variability of 3.10% between breeding programs. The UPGMA clustering divided investigated varieties into four main clusters. Based on data analysis, most distant varieties with best morphological characteristics were found which will provide valuable resource of new parent's combinations in future breeding programs. This paper also provided valuable assessment of morphological characteristics to define distinctness criteria in the DUS examination of wheat.

Key words: cultivars, DUS, similarity, wheat, variability

Corresponding author: Ivana Rukavina, Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Seed and Seedlings, Usorska 19, Brijest, 31000 Osijek, Croatia, Phone: +385 31 27 57 18; Fax: + 385 31 27 57 16; E-mail: ivana.rukavina@hcphs.hr

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp.843-852

Serbia

UDC 575
https://doi.org/10.2298/GENSR1703843S
Original scientific paper

IDENTIFICATION OF MFLP FINGERPRINT FOR HIGHER SEED ZINC ACCUMULATION IN BARLEY DH POPULATION

Behzad SADEGHZADEH^1*, Seyed Hossein JAMALI², Fatemeh VAFADAR-SHAMASBI³

¹Dryland Agricultural Research Institute (DARI); Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran

²Seed & Plant Certification & Registration Institute; Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

³Department of Agricultural Biotechnology, Maragheh Branch Islamic Azad University, Maragheh, Iran

Abstract

Sadeghzadeh B., S.H. Jamali, F. Vafadar-Shamasbi (2017): Identification of MFLP fingerprint for higher seed zinc accumulation in barley DH population. Genetika, Vol 49, No.3, 843-852.

Selection through molecular markers for seed Zn accumulation might be an efficient complementary breeding tool in barley breeding. To develop a specific molecular markers, 150 DH lines derived from a cross between Clipper (low-Zn-accumulator) and Sahara-3771 (high-Zn-accumulator) were screened under field and glasshouse conditions. Microsatellite-anchored fragment length polymorphism (MFLP) fingerprint generated by SSR-anchor primer MF128 in combination with AFLP primer MseI-AGA (5´-GATGAGTCCTGAGTAAAGA-3´) was identified as a candidate marker for tagging seed Zn accumulation gene. The sequencing of the band showed a marker of 369 bp with the sequence of SSR anchor primer MF128 and MseI-AGA at the two ends as expected. The MFLP marker associated with higher seed Zn accumulation has potential to be converted to a simple, sequence-specific, PCR-based, low-cost marker amenable to large populations, making it potentially viable for marker-assisted selection in barley breeding. This marker might be useful in the improvement of barley productivity and nutritional quality in Zn-deficient environments.

Key words: barley, MFLP, molecular marker, seed zinc concentration

Corresponding author: Behzad Sadeghzadeh (Behzada4@yahoo.com), Dryland Agricultural Research Institute (DARI), North Ring-way, Maragheh, Iran, P.O. Box: 119. Phone: +98 421 228078, Fax: +98 421 2222069

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp. 853-864

Serbia

UDC 57.633.15

https://doi.org/10.2298/GENSR1703853B

Original scientific paper

disruption of genetic IDENTITY for genebank maize accessions during conservation

Vojka babić*, Violeta andjelković, Ana nikolić, Marija milivojević,

Jelena srdić, Aleksandar popović, Natalija kravić

Maize Research Institute, Zemun Polje, Belgrade, Serbia

Abstract

babić V., V. andjelković, A. nikolić, M. milivojević, J. srdić, A. popović, N. kravić (2017): Disruption of genetic identity for genebank maize accessions during conservation.- Genetika, Vol 49, No.3, 853-864.

Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute „Zemun Polje” (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.

Key words: landraces, phenotypic markers, regeneration, viability, Zea mays L.

Corresponding author: Vojka Babić, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia, E-mail: vbabic@mrizp.rs

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 865-874

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703865Y

Original scientific paper

NUCLEOTIDE SEQUENCE VARIABILITY ANALYSIS OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II DQA1 GENE IN NIGERIAN GOATS

Abdulmojeed yakubu^1,2,3*, Adebowale E. salako³, Marcos de donato²^,4,

Michael I. takeet^2,5, Sunday O. peters^6,11, Moses okpeku⁷, Mathew wheto⁸,

Ikhide G. imumorin^2,9,10*

¹Department of Animal Science, Nasarawa State University, Lafia, Nigeria.

²Animal Genetics and Genomics Laboratory, Office of International Programs, Cornell University, Ithaca, NY 14853. USA.

³Department of Animal Science, University of Ibadan, Ibadan, Nigeria.

⁴Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Mexico.

⁵Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria.

⁶Department of Animal Science, Berry College, Mount Berry, GA 30149. USA

⁷Department of Animal Science, University of Swaziland, Swaziland.

⁸Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria.

⁹School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, 30332. USA

¹⁰African Institute for Bioscience Research and Training, Ibadan, Nigeria.

¹¹Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602. USA

Abstract

yakubu A., A. E. salako, M. de donato, M. I. takeet, S. O. peters, M. okpeku, M. wheto and I. G. imumorin (2017): Nucleotide sequence variability analysis of major histocompatibility complex class II DQA1 gene in Nigerian goats.- Genetika, Vol 49, No.3, 865-874.

Major Histocompatibility Complex (MHC) molecules loaded with peptides derived from invading pathogens are recognised by the immune system to produce a highly effective and specific response against foreign pathogens. A 310-bp fragment of exon 2 of the MHC Class II DQA1 gene was amplified in 27 animals made up of three major Nigerian goat breeds [West African Dwarf (WAD), Red Sokoto (RS) and Sahel (SH)]. Twenty amino acid polymorphic sites were found in Nigerian goats. Comparison of predicted amino acid residues of DQA1 exon 2 alleles of Nigerian goats with similar alleles from other caprine species revealed considerable congruence in amino acid substitution pattern. A significant positive selection signature was detected at the DQA1 locus of Nigerian goats in that non-synonymous substitutions occurred at a faster rate compared to synonymous substitutions (dN:dS ratio = 1.28 ; Z-Statistics= 1.634; P<0.05). The evolutionary tree constructed using UPGMA, revealed that the southern WAD goat appeared to be more related to the northern RS than SH goat at the DQA1 locus. It will be interesting therefore, for future studies to investigate the association of the genetic variants in DQA1 gene of Nigerian goats with resistance/susceptiblity to diseases in order to conserve these precious animal genetic resources.

Keywords: DQA1, MHC Class II, Nigerian goats polymorphism, positive selection.

Corresponding author: Abdulmojeed yakubu, Department of Animal Science, Nasarawa State University, Lafia, Nigeria, E-mail addresses: abdulmojyak@gmail.com; igi2@cornell.edu

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 875-890

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1703875S

Original scientific paper

GENETIC DIVERSITY OF CHARACTERISTICS IN BARLEY CULTIVARS Conservation of Forest Genetic Resources:

Key Stakeholders' Attitudes in forestry and nature protection

Mirjana šijačić-nikolić¹, Marina nonić^1*, Vedrana lalović²,

Jelena milovanović³, Jelena nedeljković¹, Dragan nonić¹

¹ University of Belgrade - Faculty of Forestry, Belgrade, Serbia

² Ministry of agriculture, forestry and water management – Forest Directorate, Belgrade, Serbia

³ University Singidunum - Faculty of Applied Ecology “Futura”, Belgrade, Serbia

Abstract

šijačić-nikolić M., M. nonić, V. lalović, J. milovanović, J. nedeljković, D. nonić (2017): Conservation of forest genetic resources: Key stakeholders' attitudes in forestry and nature protection. Genetika, Vol 49, No.3, 875-890.

Despite the large number of scientific papers dealing with conservation of forest genetic resources, research about the attitudes and evaluation of representatives of key stakeholders in the forestry and nature protection sectors, towards this issue, have not been sufficiently represented so far in Serbia. The aim of this paper was to study the attitudes of representatives of key public institutions (administrative and professional), enterprises, educational and research organizations in the forestry and nature protection sectors, towards the factors that threaten biodiversity, conservation methods, state of seed sources in Serbia and proposals of measures to improve forest genetic resources. The methodology used in the paper is an approach to the research on conservation of forest genetic resources which is used in the social sciences. The survey included a total number of 32 respondents in the territory of the Republic of Serbia. According to the key stakeholders’ opinion, urbanization and infrastructure construction (average score 4.1) have the greatest negative impact on biodiversity; conservation of forest genetic resources in natural populations through designation of seed stands is rated as the most important in situ method (average score 4.60), while establishment of seed orchards and provenance trials are rated as the most important ex situ conservation methods (average score 4.19). From the analysis of respondents' attitudes, it can be concluded that the conservation of forest genetic resources through designation of seed stands (72%) and conservation in protected areas (63%) are methods that gave the best results in Serbia. However, the majority of respondents (about 60%) stated that seed sources in Serbia do not have an appropriate structure and spatial coverage. In addition to the professional activities and support from the institutions, the majority of respondents believe that it is necessary to intensify activities to promote conservation of forest genetic resources.

Key words: biodiversity, conservation methods, ex situ, in situ, survey

Corresponding author: Marina Nonić, University of Belgrade - Faculty of Forestry, Kneza Višeslava 1, Belgrade, Tel. +381 11 3053 872, E-mail: marina.nonic@sfb.bg.ac.rs

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp. 891-910

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1703891K

Original scientific paper

PHOTOSYNTHETIC EFFICIENCY PARAMETERS AS INDICATORS OF AGRONOMIC TRAITS OF WINTER WHEAT CULTIVARS IN DIFFERENT SOIL WATER CONDITIONS

Josip KOVAČEVIĆ¹, Maja MAZUR¹, Georg DREZNER¹, Alojzije LALIĆ¹, Aleksandra SUDARIĆ¹, Krešimir DVOJKOVIĆ¹, Marija VILJEVAC-VULETIĆ¹, Marko JOSIPOVIĆ¹, Ana JOSIPOVIĆ^1*, Antonela MARKULJ-KULUNDŽIĆ¹, Hrvoje LEPEDUŠ²

¹Agricultural Institute Osijek, Osijek, Croatia

²Faculty of Humanities and Social Sciences, University of J.J. Strossmayer in Osijek,

Osijek, Croatia

Abstract

Kovačević J., M. Mazur, G. Drezner, A. Lalić, A. Sudarić, K. Dvojković, M. Viljevac-Vuletić, M. Josipović, A. Josipović, A. Markulj-Kulundžić, H. Lepeduš (2017): Photosynthetic efficiency parameters as indicators of agronomic traits of winter wheat cultivars in different soil water conditions.- Genetika, Vol 49, No.3, 891-910.

In an effort to find breeding methods for improving drought stress tolerance and grain yield, twelve photosynthetic efficiency parameters have been measured on ten cultivars of winter wheat (Triticum aestivum L.), along with water use (WU), water use efficiency (WUE) and agronomic traits of grain yield (GYP), biomass weight (BWP), harvest index (HI), yield stability index (YSI) and stress tolerance index (STI) in the vegetative pot trial with control (B1) and drought stress (B2) treatments. Drought stress induced in three different stages of development has caused decrease in water use efficiency based on biomass (WUEb) (B1: 2.94 g L^-1; B2: 2.71 g L^-1) and grain yield (WUEg) (B1: 1.03 g L^-1; B2: 0.89 g L^-1), as well as GYP and BWP. Dissipation energy flux per excited cross section (DI₀/CS₀) observed in the drought stress treatment in the tillering stage of growth gave significant negative correlation coefficient (P≤0.05) with agronomic traits of tested wheat cultivars (GYP:-0.75; WUEg and STI: -0.74; YSI: -0.67). Performance index (PI_ABS) measured in the drought stress conditions in the flag leaf stage was in significant positive correlation with GYP and WUEg (r=0.64). Lower values of absorption flux per excited cross section (ABS/CS₀), electron transport per excited CS (ET₀/CS₀) and dissipation energy flux per excited CS (DI₀/CS₀) and higher values of PI_ABS, measured on wheat genotypes (cultivars) in the drought stress conditions of pot trial, could indicate higher tolerance to drought stress conditions. Results of the studied photosynthetic efficiency parameters of wheat cultivars were also the good predictor for important agronomic traits, especially, when they were detected in the early stage of growth.

Key words: agronomic traits, drought stress tolerance, photosynthetic parameters, Triticum aestivum L., wheat cultivars.

Corresponding author: Ana Josipović, Agricultural Institute Osijek, Južno predgrađe 17, 31 103 Osijek, Croatia; Phone +385 31 515 537; E-mail: ana.josipovic@poljinos.hr

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 911-920

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703911M

Original scientific paper

GLUTATHIONE S-TRANSFERASE M1 - T1 NULL GENOTYPES AND SUSCEPTIBILITY TO HODGKIN’S LYMPHOMA

Amin moosavi¹ , Mohammad FORAT YAZDI², *Masoud DEHGHAN TEZERJANI³, Mohammad Hasan SHEIKHHA⁴, Seyed Mahdi HOSEINI⁵ , Fatemeh MOEININIA⁶ ,

Mahnaz ZOHAL¹, Ali MOUSAVI¹

¹ Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

²Department of Oncology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

³ Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

⁴Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

⁵ Cell & Molecular Biology Division, Cell Biology Department, Ferdowsi University of Mashhad, Mashhad, Iran.

⁶ Department of Internal Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

Abstract

moosavi A., M. Forat Yazdi, M. Dehghan Tezerjani, M. H. Sheikhha, S. M. Hoseini, F. Moeininia, M. Zohal, A. Mousavi (2017): Glutathione S-transferase M1 - T1 null genotypes and susceptibility to Hodgkin’s lymphoma.- Genetika, Vol 49, No.3, 911- 920.

Hodgkin’s Lymphoma (HL) is a heterogeneous malignant disease of lymph node. The glutathione S-transferases (GSTs) have an important role in the detoxification of a wide variety of toxins and carcinogens. Studies have been indicated that genetic variation in the GST gene family may lead to susceptibility in HL. Hereby, we investigated the association of GSTT1 and GSTM1 null genotypes with HL in the Iranian population. This case-control study consisted of 76 patients suffering from HL and 120 healthy individuals as a control group. Genomic DNA was extracted and genotyping of GSTT1 and GSTM1 genes for the identification of their null genotypes was carried out using multiplex PCR method. Our findings indicated that GSTM1 null genotype is associated with risk of developing HL in our population (P=0.025; OR=2.00; 95%CI=1.110- 3.602); however, no association was found for GSTT1 null genotype. Our study also showed that the GSTM1 null genotype increased the risk of disease in the individuals younger than 45 years, and it had a positive association with low ESR. GSTM1 null genotype may have the key role in increasing the risk of HL in the Iranian population.

Key word: Hodgkin’s Lymphoma, glutathione S-transferases, GSTM1, GSTT1, Multiplex PCR

Corresponding author: Masoud Dehghan Tezerjani; Research and Clinical Center for infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Email:Masoud-Dehghan@hotmail.com

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp. 921-934

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1703921J

Original scientific paper

MOLECULAR CHARACTERIZATION OF Cronobacter sakazakii ISOLATED

FROM DIFFERENT HERBAL TEAS AND MIXTURES IN SERBIA

Dragana jošić¹, Marija stojanović², Zorica lepšanović³, Vera katić⁴

¹Institute of Soil Science, Genetic Lab, Belgrade, Serbia

²Center for Food Analyses, Belgrade, Serbia

³MilitaryMedical Academy, Institute of Epidemiology, Belgrade, Serbia

⁴Faculty of Veterinary Medicine, Belgrade, Serbia

Abstract

jošić D., M. stojanović, Z. lepšanović, V. katić (2017): Molecular characterization of Cronobacter sakazakii isolated from different herbal teas and mixtures in Serbia.- Genetika, Vol 49, No.3, 921- 934.

Cronobacter sakazakii is an important cause of human infections that can be serious and even fatal among premature neonates and immunocompromised adults or infants. Because of its high tolerance to osmotic stress, C. sakazakii is frequently isolated from dried foods, such as powdered infant formula and herbal teas. The aim of investigation was detection, identification and molecular characterization of Cronobacter sakazakii isolates from infant formula and various herbal teas collected from Serbian market and tested for import control. C. sakazakii was not detected in any of the 360 analysed samples of powdered infant formula. However, 192 out of 520 samples of herbal teas tested were positive for C. sakazakii (37.1%). The high prevalence was observed in teas for children (51.6%) and in “baby” teas (44.1%), followed by medicinal teas (38%). The largest one-herb-teas group (221 samples) contained 72 C. sakazakii-positive samples (32.6%) and involved Sennae folium, Althaeae radix, Menthae piperitae folium, Chamomilae flos and Urticae folium teas. Molecular characterization of isolated C. sakazakii from different herbal teas by rep-PCR, RAPD and 16S rRNA sequences analysis showed the high similarity to C. sakazakii NCTC 8155. Knowing this strain as one of the most pathogenic clinical strains, our results raise concern about the safety risks these foods pose to immunocompromised and healthy consumers, especially for babies and children.

Key words: Cronobacter sakazakii, herbal tea, infant formula, RAPD, rep-PCR, 16S rRNA

Corresponding author: Dragana Jošić; Institute of Soil Science, Genetic Lab, Teodora Drajzera 7, Belgrade, Serbia; tel. 069/8223531; e-mail:dragana.josic@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 935-957

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703935B

Original scientific paper

GENETIC DIVERSITY OF FARMER-PREFERRED COWPEA (Vigna unguiculata L. Walp) LANDRACES IN TURKEY AND EVALUATION OF THEIR RELATIONSHIPS BASED ON AGROMORPHOLOGICAL TRAITS

Mehmet Kadri bozokalfa*, Tansel kaygisiz aşçioğul, Dursun eşiyok

Department of Horticulture, Faculty of Agriculture, Ege University, İzmir, Turkey

Abstract

bozokalfa M.K., T. kaygisiz aşçioğul, D. eşiyok (2017): Genetic diversity of farmer-preferred cowpea (Vigna unguiculata L. walp) landraces in Turkey and evaluation of their relationships based on agromorphological traits.- Genetika, Vol 49, No.3, 935- 957.

Cowpea (Vigna unguiculata L. Walp) is one of the important vegetable, grain, and fodder legume species grown in the tropics and subtropics. Cowpea is grown on small farms, and locally adapted landraces or populations are cultivated, and genetic improvements are limited by the lack of knowledge of genetic diversity of the indigenous and cultivated germplasm. Characterization and classification of diversity of the germplasm is valuable for both plant breeders and germplasm curators in the development of conservation strategies and identification of plant genetic resources. In the present study, 36 qualitative and quantitative agromorphological traits were used to characterize and assess the genetic diversity of 32 farmer preferred cowpea genotypes collected from diverse cultivated locations. Genetic variation was highly significant, and phenotypic diversity was observed for agromorphological traits. Agromorphological traits were classified by principal components analysis (PCA) into 10 components, which explained 81.18% of the total variation. PCA revealed that seed properties such as weight, diameter, width, length, eye color and immature pod pigmentation, leaf and pod color properties were the primary characteristics to discriminate cowpea genotypes. The hierarchical analysis grouped the genotypes into five clusters, and significant association was not apparent between geographic origin and agromorphological traits. The promising germplasm was identified to improve the landraces for fresh pod, grain, and fodder.

Key words: Characterization, cowpea, diversity, genetic resources, variability

Corresponding author: Mehmet Kadri bozokalfa, Department of Horticulture, Faculty of Agriculture, Ege University, İzmir, Turkey, Tel & Fax: +90 232 3881865,e-mail: mehmet.kadri.bozokalfa@ege.edu.tr

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 959-968

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703959K

Original scientific paper

ACTN3 POLYMORPHISM PREVALENCE IN GENERAL POPULATION

OF SIX BALKAN COUNTRIES

Hacer konakli¹, Zafer konakli², Senol dogan¹, Damir marjanovic^{1, 3},

Serkan dogan^{1, 4, *}

¹Department of Genetics and Bioengineering, International Burch University, Sarajevo,

Bosnia and Herzegovina

²Department of International Business, International Burch University, Sarajevo, Bosnia and Herzegovina

³Institute for Anthropological Research, Zagreb, Croatia

⁴Institute for Biomedical Diagnostics and Research NALAZ, Sarajevo, Bosnia and Herzegovina

Abstract

konakli H., Z. konakli, S. dogan, D. marjanovic, S. dogan (2017): ACTN3 polymorphism prevalence in general population of six Balkan countries. - Genetika, Vol 49, No.3, 959-968.

The aim of this study was to investigate the prevalence of RR, RX and XX genotypes of rs1815739 single nucleotide polymorphism of ACTN3 gene in general population of six Balkan countries. This SNP is currently thought to give genetic predisposition for advantageous sport performance, with its R allele positively correlating with better performance in strength/power sports, and X allele being present in higher frequency in elite athletes in endurance disciplines. A total of 483 individuals (281 males and 202 females) from seven populations from six countries was tested. Samples were collected by buccal swabbing method, DNA was isolated according to salting-out protocol and genotyping was performed using PCR and RFLP analysis. Obtained results suggest that RR and RX genotypes (43.7% and 44.1%, respectively) are overrepresented when compared to XX genotype (12.2%). The XX genotype in the study samples is present in lower frequency when compared to the global (16-18%) and European (18%) average. This study reports the first population data on genotype prevalence in sports-related genes for this part of Europe and is the beginning of research interests that intend to investigate genetic predispositions of elite athletes from this region competing internationally in different disciplines.

Key words: ACTN3, endurance disciplines, rs1815739, strength/power disciplines

Corresponding author: Serkan Dogan, Department of Genetics and Bioengineering, International Burch University, Sarajevo, Bosnia and Herzegovina, E-mail: serkan.dogan@ibu.edu.ba, Phone: +387 33 944 494

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp.969-977

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575
https://doi.org/10.2298/GENSR1703969Y

Original scientific paper

THE UTERINE MILK PROTEIN (UTMP) GENE IS ASSOCIATED WITH MILK PRODUCTION TRAITS IN IRANIAN HOLSTEIN BULLS

Ahad yousefi¹^*, Abdolreza salehi¹, Mehdi aminafshar², Mohammad bagher sayyadnejad³

^{^[1]}Department of Animal and Poultry Science, Aburaihan Campus, University of Tehran, Tehran, Iran.

² Department Of Animal Science, Faculty of Agriculture and Natural Recourse, Science and Research Branch, Islamic Azad University, Tehran, Iran.

³ Animal Breeding Center, Karaj, Iran.

Abstract

yousefi A., A. salehi, M. aminafshar, M. bagher sayyadnejad (2017): The uterine milk protein (UTMP) gene is associated with milk production traits in Iranian Holstein bulls. - Genetika, Vol 49, No.3, 969-977.

Uterine Milk Protein (UTMP) the member of Serine Protease Inhibitor superfamily secreted from uterine endometrium during pregnancy under influence of progesterone. Specific functions of UTMP include protease inhibition, growth control, and direct nutrition of the conceptus. This investigation was performed in order to study polymorphism of UTMP gene and its association with milk production traits in Iranian Holstein bulls. Genomic DNA was extracted from 100 semen samples of proven bulls. In order to amplify 568bp-fragment including same part of intron 3, whole exon 4 and downstream of UTMP gene, a pair of primer was designed. PCR products were digested with BsrI enzyme. The allele frequencies of a, b and c were 0.135, 0.69 and 0.175, respectively in studied population. Four genotypes AB, BB, BC and CC were observed with frequencies of 0.27, 0.49, 0.13 and 0.11, respectively. Genotypes AA and AC were not observed in this study. The chi-square (X²) test revealed deviation from Hardy-Weinberg equilibrium. Data were obtained from animal breeding centre of Iran for the first lactation during 1993-2008 to estimate some genetic parameters. Heritability of milk yield, fat yield, protein yield, fat percent and protein percent were 0.28, 0.21, 0.22, 0.32 and 0.34 respectively. Results indicated significant association between UTMP Genotypes and milk, fat and protein yield traits. Meanwhile, Bulls with CC Genotype had higher milk, fat and protein yield compared to other genotypes (p<0.05). These results suggested UTMP as a candidate gene influencing milk production traits might be implemented in breeding programs to improve the production performance of Iranian Holstein cattle.

Key words: UTMP gene, Heritability, Polymorphism, Milk production, Holstein cattle

Corresponding author: Abdolreza Salehi, Department of Animal and Poultry Science, Aburaihan Campus, University of Tehran, Tehran, Iran, E-mail: arsalehi@ut.ac.ir , TEL : +982136040907

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 979-988

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703979P

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY OF SILVER FIR (Abies alba Mill.) IN SERBIA USING SSR MARKERS

Vladan popović¹, Aleksandar lučić¹, Ljubinko rakonjac¹, Branislav cvjetković³, Snežana mladenović drinić², Danijela ristić²

¹Institute of Forestry, Belgrade

²Maize Research Institute, Zemun Polje, Belgrade

³Faculty of Forestry, Banja Luka

Abstract

popović V., A. lučić, Lj. rakonjac, B. cvjetković, S. mladenović drinić, D. ristić (2017): Assessment of genetic diversity of silver fir (Abies alba mill.) in Serbia using SSR markers - Genetika, Vol 49, No.3, 979-988.

The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubočica Bare and Stara Planina; Dubočica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara).

A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.

Key words: Abies alba Mill., SSR markers, population, variability.

Corresponding author: Vladan Popović; Institute of Forestry; Kneza Višeslava 3; 11000 Belgrade; Serbia; Phone: 011-3553355; e-mail: vladanpop79@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 989-1000

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1703989S

Original scientific paper

IRAP variability in Prunus domestica (L.) Borkh based on Cassandra retrotransposon polymorphism

Slavomíra senkovÁ¹, Jana ŽiarovskÁ¹, eloy FernÁndez^2*, Danka boŠeĽovÁ¹, Milan beŽo¹

¹ Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Slovak Republic

² Faculty of Tropical AgriSciences, Czech University of Life Sciences in Prague, Czech Republic

Abstract

senková S., J. Žiarovská, e. Fernández, D. bošeľová, M. bežo (2017) : IRAP variability in Prunus domestica (L.) Borkh based on Cassandra retrotransposon polymorphism. - Genetika, Vol 49, No.3, 989-1000.

Plum species are reported to possess a wide genomic variability and that is why DNA markers are still actual in the characterization of its germlasm. In this study, twenty-three genotypes of European plums were assessed for the amplified length based polymorphism among the retrotransposon Cassandra insertions in their genomes. The obtained insertional polymorphism caused by the activity of Cassandra showed regional and pedigree differences in the analysed accessions of European plums. Two primers were used in analysis. The first resulted in the amplification in 203 amplicons and the 86.6 % polymorphism. Two unique fragments were obtained for the Torysa and Podolínec varieties using this primer. The second primer resulted in the amplification in 267 amplicons and 74.2 % polymorphism. Four unique fragments were obtained for the plum varieties Švestka domácí, Čačanská ranná and Elena. Hierarchical cluster analysis divided the analysed accessions into the four main clusters. To show the length polymorphism diferences of the analysed genotypes from Germany, Slovakia and former Yugoslavia more pecisely, the scattergram for them was constructed.

Key words: DNA polymorphism; European plum; retrotransposon marker; variability

Corresponding author: Eloy Fernández, Faculty of Tropical AgriSciences, Czech University of Life Sciences in Prague, Czech Republic, e-mail: eloy@ftz.czu.cz

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1001-1014

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1703001H

Original scientific paper

GENETIC PARAMETERS AND COMBINING ABILITY OF SOME IMPORTANT TRAITS IN RICE (Oryza sativa L.)

Alireza HAGHIGHI HASANALIDEH¹, Ezatollah FARSHADFAR¹*,

Mehrzad ALLAHGHOLIPOUR²

¹Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.

²Assistant Professor of Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization, Rasht. Iran.

Abstract

Haghighi HasanalidehA., E. Farshadfar, M. Allahgholipour (2017): Genetic parameters and combining ability of some important traits in rice (Oryza sativa L.). - Genetika, Vol 49, No.3, 1001-1014.

In order to study the combining ability, genetic parameters and gene actions of yield, yield components and quality characters in rice, fifteen F₂ generation of a 6×6 diallel cross, excluding reciprocals, was grown in a randomized complete block design (RCBD) with three replications. The results of analysis of variance showed significant differences between the genotypes for grain yield (GY), 100-grain weight (HGW), number of panicles per plant (PN), panicle length (PL), number of full grains per panicle (FGN) and for quality characters including amylose content (AC) and gel consistency (GC). The results of combining ability analysis revealed that general combining ability (GCA) and specific combining ability (SCA) were significant for characters GY, FGN, GC, AC, HGW and PN indicating the involvement of additive and non-additive effects in their inheritance, however high amounts of Bakers ratio remarked that additive gene effect had more portion in controlling these traits. The best combiners for GY, HGW, FGN, PN and PL, were RI18447-2, IR 50, Daylamani, RI18430-46 and Daylamani respectively. For AC and GC, the best combiner was Daylamani. Hayman's graphs showed that regression line passed below the origin cutting W_r axis in the negative region for HGW, PN, PL and GC, indicating the presence of over dominance. Estimates of genetic parameters showed significant amount of H1 and H2, and non-significant amount of D for the characters GY, PN, PL and GC, which confirmed the existence of dominance in the inheritance of these traits.

Key words: combining ability, diallel analysis, F₂progenies, genetic parameters, rice.

Corresponding author: Ezatollah Farshadfar, Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, Tel: +98 8338320111; Mobile No: +98 9183893472; Fax No: +98 8338323731, E-mail: e_farshadfar@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1015-1021

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703015H

Original scientific paper

DIRECT AND INDIRECT EFFECTS OF MORPHOPHYSIOLOGICAL TRAITS

ON KERNEL PROTEIN CONTENT OF CONFECTIONARY SUNFLOWER

Nada hladni, Milan jocković, Siniša jocić, Vladimir miklič,

Dragana miladinović, Miroslav zorić

Institute for Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Hladni N., M. Jocković, S. Jocić, V. Miklič, D. Miladinović, Miroslav Zorić (2017): Direct and indirect effects of morphophysiological traits on kernel protein content of confectionery sunflower.- Genetika, Vol 49, No.3, 1015-1021.

The most important criterion for introducing new confectionary sunflower hybrids into production is high protein yield. In the breeding process it is important to identify traits which could be used as selection criteria for increased kernel protein content. Increase of kernel protein content results in increased protein yield. This research was conducted during three vegetation seasons on 22 NS high-protein two-line confectionary sunflower hybrids produced within the breeding program at IFVCNS, Novi Sad, Serbia. Strong and very strong correlations were found among the largest number of examined traits. Based on the analysis of simple correlation coefficients, strong negative correlation was determined between kernel protein content and kernel ratio (-0.516^*). A weak negative interdependence was determined between head diameter, seed protein content, and kernel protein content. Positive but weak correlation was determined between kernel protein content and thickness of seed, length of seed, width of seed, and 1000 seed weight. Path coefficient analysis for kernel protein content at phenotypic level showed that the thickness of seed had a strong positive direct effect on kernel protein content (DE=382^*). Kernel ratio and width of seed had a very strong direct negative effect on kernel protein content (DE=-0.990^**; DE=0.600^**). A weak direct positive effect of head diameter, seed protein content and length of seed was established, whereas 1000 seed weight had a weak direct negative effect on kernel protein content. Path coefficient analysis indicates showed that the thickness of seed has high great influence on kernel protein content, and an important selection criterion for breeding for high protein yield.

Key words: confectionary sunflower, correlations, kernel protein content, path coefficient analysis, quantitative traits.

Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: +381 21 4898 411; Fax: +381 21 6413 833; e-mail: nada.hladni@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1023-1034

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1703023B

Original scientific paper

MAIZE GERMPLASM OF EASTERN CROATIA WITH NATIVE RESISTANCE

TO WESTERN CORN ROOTWORM (Diabrotica virgifera virgifera LeConte)

Andrija BRKIĆ¹, Ivan BRKIĆ¹, Antun JAMBROVIĆ¹, Marija IVEZIĆ², Emilija RASPUDIĆ²,

Mirjana BRMEŽ², Zvonimir ZDUNIĆ¹, Tatjana LEDENČAN¹, Josip BRKIĆ¹, Monika MARKOVIĆ³,

Goran KRIZMANIĆ⁴, Domagoj ŠIMIĆ¹

¹ Agricultural Institute Osijek, Department for Breeding and Genetics of Maize, Osijek, Croatia

² Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Department for Plant Protection, Chair for Entomology and Nematology, Osijek, Croatia

³ Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Department for Plant Production, Chair for General Plant Production and Agricultural Melioration, Osijek, Croatia

⁴ Agricultural Institute Osijek, Department for Breeding and Genetics of Forage Crops, Osijek, Croatia

Abstract

Brkić A., I. Brkić, A. Jambrović, M. Ivezić, E. Raspudić, M. Brmež, Z. Zdunić, T. Ledenčan, J. Brkić, M. Marković, G. Krizmanić, D. Šimić (2017): Maize germplasm of Eastern Croatia with native resistance to western corn rootworm (Diabrotica virgifera virgifera leconte).- Genetika, Vol 49, No.3, 1023-1034.

The western corn rootworm (Diabrotica virgifera virgifera LeConte; WCR) is a serious maize pest in Croatia. The species was first registered in Europe in the early 1990s and since then became one of the most dangerous maize pests, especially in parts of Central and Southeast Europe. Larvae that feed on the maize roots cause the most serious damages in maize fields. Management of this pest is difficult and expensive, with possible serious impact on the environment. Native (or host-plant) resistance of maize against WCR could provide new economically and ecologically sustainable options in WCR management. Main goal of this study was to assess the variability of maize germplasm, correlations among resistance traits, and detect potential sources of resistance that could be used in breeding programs in order to develop hybrids with higher level of resistance against WCR. To our knowledge, the first native resistant hybrid is yet to be registered. Results showed great variability of estimated germplasm. Effect of the genotype was significant in all environments, as well as many interactions between genotype and the environment. Significant interactions emphasize the importance of the environment in WCR native resistance research. Significant positive correlations among all traits were detected. Several inbred lines were selected as a potentially useful germplasm for resistance breeding programs.

Key words: germplasm, maize, native resistance, root traits, western corn rootworm

Corresponding author: Andrija Brkić, Agricultural Institute Osijek, Department for Breeding and Genetics of Maize, Južno predgrađe 17, Osijek 31000, Croatia. Telephone: +385 31 515 588. E-mail: andrija.brkic@poljinos.hr

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1035-1045

Serbia

UDC 575

https://doi.org/10.2298/GENSR1703035M

Original scientific paper

MORPHOGENETIC VARIABILITY AND GENETIC LOADS AMONG PATIENTS WITH DIFFERENT EXPRESSION OF DEVELOPMENTAL HIP DYSPLASIA

Sonja MILAŠINOVIĆ¹, Suzana CVJETIĆANIN^2,3, Radivoj BRDAR^2,4, Dejan NIKOLIĆ^2,5

¹Institute for Children's Disease, Clinical Centre of Montenegro, Podgorica, Montenegro

²School of Medicine, University of Belgrade, Belgrade, Serbia

³Institute of Human Genetics, School of Medicine, University of Belgrade, Belgrade, Serbia

⁴Pediatric Surgery Department, University Children’s Hospital, Belgrade, Serbia

⁵Physical Medicine and Rehabilitation Department, University Children’s Hospital, Belgrade, Serbia

Abstract

Milašinović S., S. Cvjetićanin, R. Brdar, D. Nikolić (2017): Morphogenetic variability and genetic loads among patients with different expression of developmental HIP dysplasia.- Genetika, Vol 49, No.3, 1035-1045.

Assuming that developmental hip dysplasia (DDH) is a genetically controlled disease, we made a hypothesis that an increased homozygosity level and genetic loads, among the patients with DDH, could be some kind of predisposition for the degree of illness expression. Using HRC-test (test for determination of homozygously recessive characteristics in humans) we analyzed presence, distribution, and individual combination of 20 selected genetically controlled morphophysiological traits among DDH patients (N=200) and controls (N=200). Among groups of DDH patients the increase in the degree of genetic homozygosity correlates with the degree of illness expression (dysplasia/subluxations-39%; luxations-45%). There is significant difference in mean HRC value between tested groups of patients with dysplasia/luxations (p<0.05) and subluxations/luxations (p<0.05). Our results showed increase of morphogenetic homozygosity in the group of patients with bilateral hip dislocation (BL) (45%), compared to group with unilateral expression (UL) (41%) and control (23.5%). There is significant difference in mean HRC value between tested groups of patients with UL and BL (p<0.05). Also, surgically treated patients showed significant increase of genetic homozygosity (43%) compared to those conservatively treated (37%) and controls. We found significant difference in mean HRC value between tested groups of patients that were treated conservatively and surgically (p<0.05). Our results showed increase of genetic homozygosity in the groups of DDH female patients (44%), compared to male patients (39%), while in controls there was no difference between gender. Female DDH patients show a significant increase in average homozygosity of tested genetic markers (p<0.05) than male DDH patients. The enlargement of genetic loads correlates with severity of the disease (in studied groups of DDH patients) which may indicate some kind of predisposition for the degree of illness expression.

Key words: developmental hip dysplasia, genetic loads, homozygously recessive characteristics, morphogenetic variability

Corresponding author: Dejan Nikolic, University Childrens Hospital, School of Medicine, University of Belgrade, Belgrade, 27 Marta 6A, Belgrade, Serbia; Phone: +381638133345, E-mail: denikol27@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp 1047-1057

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703047B

Original scientific paper

MOLECULAR DETECTION AND IDENTIFICATION OF ALFALFA MOSAIC VIRUS (AMV) ON PEPPER CULTIVATED IN OPEN FIELDS IN R. MACEDONIA

Katerina BANDJO ORESHKOVIKJ^1,a, Rade RUSEVSKI², Biljana KUZMANOVSKA², Mirjana JANKULOVSKA³, Zoran T. POPOVSKI⁴

¹ Department of Plant Protection, Institute of Agriculture, University “Ss. Cyril and Methodius” Skopje, R. Macedonia

² Department of Phytopathology, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius” Skopje, R. Macedonia

³ Department of Genetics and Plant Breeding, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius” Skopje, R. Macedonia

⁴ Department of Biochemistry and Genetic Engineering, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius” Skopje, R. Macedonia

Abstract

Bandjo Oreshkovikj K., R. Rusevski, B. Kuzmanovska, M. Jankulovska, Z. T. Popovski (2017): Molecular detection and identification of alfalfa mosaic virus (AMV) on pepper cultivated in open fields in R. Macedonia.- Genetika, Vol 49, No.3, 1047-1057.

Alfalfa mosaic virus (AMV) is one of the most distributed and economically important plant viruses in pepper in R. Macedonia. Serological detection of AMV in eight important pepper production regions in R. Macedonia and molecular identification of a representative isolate were performed. The virus detection of AMV was conducted using DAS-ELISA method. In order to make molecular detection, RT-PCR was performed. Phylogenetic analysis was conducted, based on the partial sequences of the coat protein gene. A genetic relationship of the Macedonian isolate KUA7-2013 gained in this study was compared with 29 AMV isolates from other parts of the world. High level of nucleotide (92 – 94.4%) and amino acid identities (91.9 – 97.1%) were determined. In the constructed phylogenetic tree, the Macedonian AMV isolate was clustered in group II together with isolates from France and Great Britain. In this study, for the first time in R. Macedonia, an isolate of AMV was identified at the molecular level.

Key words: Alfalfa mosaic virus, pepper, molecular detection, phylogenetic analysis, coat protein gene sequences.

Corresponding author: Katerina Bandjo Oreshkovikj, Institute of Agriculture, blvd.”16-ta Makedonska Brigada” no.3^A, 1000 Skopje, Republic of Macedonia, tel: +389 2 3230910, fax: +389 2 3114283, e-mail: kbandzo@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1059-1070

UDC 575

https://doi.org/10.2298/GENSR1703059K

Original scientific paper

GENOTYPING OF Trichomonas vaginalis ISOLATES FROM WOMEN

IN SHAHREKORD CITY (SOUTHWESTERN IRAN)

Bahman khalili¹^*, Payam ghasemi-dehkordi², Gholamreza pourshahbazi³, Hossein yousofi-darani⁴, Morteza hashemzadeh-chaleshtori³, Abbas doosti⁵

¹Department of Parasitology and Mycology, Faculty of Medicine, Shahrekord University

of Medical Sciences, Shahrekord, Iran

²Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

³Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

⁴Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

⁵Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

khalili B., P. ghasemi-dehkordi, G. pourshahbazi, H. yousofi-darani, M. hashemzadeh-chaleshtori, A. doosti (2017): Genotyping of Trichomonas vaginalis isolates from women in Shahrekord city (Southwestern Iran).- Genetika, Vol 49, No.3, 1059-1070.

Trichomonas vaginalis is a causative agent of vaginitis in female and urethritis in men. It is primarily transmitted by sexually route. It is known that each geographical area has its own set of Trichomonas vaginalis strain. Parasite strains in each region have its specific characterizations and different strains of the parasite are able to cause various diseases with the acuity and severity. The aim of this study was to determine the genotyping of Trichomonas vaginalis strains in the Shahrekord city (Chaharmahal Va Bakhtiari province, southwest Iran). A total of 1725 vaginal samples were taken from clinically suspected women for Trichomonas vaginalis infection and 21 specimens were diagnosed as positive by direct smear wet mount and culture repeated passage of the parasite in the modified TYI-S-33 medium. The genomic DNA was extracted from each sample and the nested polymerase chain reaction was applied using specific oligonucleotide primers for actin gene amplification. Finally, the restriction fragment length polymorphism using RsaI, MseI, and HindII restriction enzymes were done on PCR products for genotyping. PCR-RFLP analysis of 21 positive cases (1.22%) was showing the most frequent genotype was H (8 cases), followed by G (4 cases), E (3 cases), and P(2 cases). N and I genotypes were detected in each 1 case. Also, there was 2 cases mix (E and H) genotype. The findings of the present work were showed 7 different genetic strains in isolated Trichomonas vaginalis from symptomatic and asymptomatic women in Shahrekord city. In this study high level of H genotype in referred women in Shahrekord city was observed and H, G, E, and I genotypes were may be related to burning and itching as well as H, P, and mix genotypes were associated with malodorous discharge with pelvic pain in this region of Iran. For a suggestion, it would be better in further studies the accurate determination of genetic diversity of this parasite done in Chaharmahal Va Bakhtiari province and other parts of the country.

Key words: Trichomonas vaginalis, genotyping, polymerase chain reaction, restriction fragment length polymorphism, Shahrekord

Corresponding author: Bahman Khalili, Department of Parasitology and Mycology, Faculty of Medicine, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran, Postal code: 8813833435, tel: +98-38-33334691, fax: +98-38-33334911. E-mails: bahman55_khalili@yahoo.com

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Published in „GENETIKA“ Vol. 49, No. 3 (2017), pp. 1071-1080

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1703071Q

Original scientific paper

ASSESSMENT OF GENETIC VARIABILITY IN Trigonella Foenum-graecum L. GERMPLASM BY SDS-PAGE ANALYSIS

Abdul QADIR¹, Niaz ALI¹, Sohail Ahmad JAN^{*2, 3}, Malik Ashiq RABBANI²,

Muhammad Ishaq IBRAHIM²,Ashtar KHAN² and Haris KHURSHID⁴

¹Department of Botany, Hazara University, Khyber Pakhtunkhwa, Pakistan

²Plant Genetic Resources Institute (PGRI), NARC, Islamabad, Pakistan

³Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

⁴Oilseeds Research Program, NARC, Islamabad, Pakistan

Abstract

Qadir A., N. Ali, S. A. Jan, M. A. Rabbani, M. I. Ibrahim,A. Khan and Haris Khurshid (2017): Assessment of genetic variability in Trigonella foenum-graecum L. germplasm by SDS-PAGE analysis.- Genetika, Vol 49, No.3, 1071-1080.

The 96 genotypes of Trigonella foenum-graecum L. (Fenugreek) were estimated by using Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE). The accessions were maintained from diverse ecological areas of the world. Total seed storage proteins were electrophoresis on 12.5% polyacrylamide gels. A total of 17 protein bands were detected, of which seven were highly polymorphic and six were moderate polymorphic and four were low polymorphic with molecular weight extending from of ~15 to ~180 kDa . The dendrogram based on similarity matrix using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all the genotypes into four major clusters i.e., I, II, III and IV comprising 51, 24, 10 and 11 accessions, respectively. Although limited genetic diversity was detected amongst known germplasm but the presence/absence of polymorphic bands revealed a significant variances among different Trigonella genotypes. The differences exposed in this project work should be oppressed for the future breeding prospective of Trigonella genotypes by using other advanced molecular techniques. The SDS-PAGE per se seems inadequate tool for such kind of analysis, and may be integrated with other genetic and sequence based approaches for more precise estimation of the genetic variability within closely related accessions. Our results provide baseline for obtaining locally adapted and improved cultivars of fenugreek in Pakistan.

Key words: Fenugreek,genetic variation, SDS-PAGE, UPGMA

Corresponding author: Sohail ahmad jan, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan, e-mail: sjan.parc@gmail.com, sohailahmadjan3@gmail.com

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Published in „GENETIKA“ Vol. 49, No. 3(2017), pp. 1081-1093

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703081Z

Original scientific paper

Novel Fluorescent Sequence-related Amplified Polymorphism (FSRAP) Markers for the Construction of a Genetic Linkage Map

of Wheat (Triticum aestivum L.)

Lingbo Zhao^1#, Li Zhang^1#, Jipeng Qu^2#, Yan Yu¹, Lu Lu³, Zhengsong Peng²,

Wuyun Yang⁴, Shuhong Wei¹, Jun Yang¹, Zaijun Yang¹

¹ Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of

Education, China West Normal University, Nanchong, Sichuan, China.

²School of Agricultural Science, Xichang College, Xichang, Sichuan, China.

³College of Environmental Science and Engineering, China West Normal University,

Nanchong, Sichuan, China.

⁴Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of

Agricultural Sciences, Chengdu, Sichuan, China.

Abstract

Zhao L., L. Zhang, J. Qu, Y. Yu, L. Lu, Z. Peng, W. Yang, S. Wei, J. Yang, Z. Yang(2017): Novel fluorescent sequence-related amplified polymorphism (FSRAP) markers for the construction of a genetic linkage map of wheat (Triticum aestivum L.).- Genetika, Vol 49, No.3, 1081-1093.

Novel fluorescent sequence-related amplified polymorphism (FSRAP) markers were developed based on the SRAP molecular marker. Then, the FSRAP markers were used to construct the genetic map of a wheat (Triticum aestivum L.) recombinant inbred line population derived from a Chuanmai 42 × Chuannong 16 cross. Reproducibility and polymorphism tests indicated that the FSRAP markers have repeatability and better reflect the polymorphism of wheat varieties compared with SRAP markers. A total of 430 polymorphic loci between Chuanmai 42 and Chuannong 16 were detected with 189 FSRAP primer combinations. A total of 281 FSARP markers and 39 SSR markers were classified into 20 linkage groups. The maps spanned a total length of 2499.3cM with an average distance of 7.81cM between markers. A total of 201 markers were mapped on the B genome and covered a distance of 1013cM. On the A genome, 84 markers were mapped and covered a distance of 849.6cM. On the D genome, however, only 35 markers were mapped and covered a distance of 636.7cM. No FSRAP markers were distributed on the 7D chromosome. The results of the present study revealed that the novel FSRAP markers can be used to generate dense, uniform genetic maps of wheat.

Keywords: genetic linkage map, FSRAP markers, RIL population, SRAP markers, wheat

Corresponding author: Zaijun Yang; Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, China West Normal University, Nanchong, Sichuan, China; Phone: +86172568352; e-mail: yangzaijun1@126.com

^#Contributed equally to this work.

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Published in „GENETIKA“ Vol. 49 No. 3 (2017), pp. 1095-1104

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703095S

Original scientific paper

INFLUENCE OF GENOTYPE AND NITROGEN NUTRITION ON GRAIN SIZE VARIABILITY IN SPRING MALTING BARLEY

Vladanka STUPAR¹ Aleksandar PAUNOVIĆ², Milomirka MADIĆ²

Desimir KNEŽEVIĆ³

¹Higher Education Technical School of Professional Studies Požarevac, Pozarevac, Serbia

²University of Kragujevac, Faculty of Agronomy, Cacak, Serbia

³University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lesak,

Kosovo and Metohia, Serbia

Abstract

Stupar V., A. Paunović, M. Madić, D. Knežević (2017): Influence of genotype and nitrogen nutrition on grain size variability in spring malting barley.- Genetika, Vol 49, No.3, 1095-1104.

Grain size is an important quality parameter of malting barley, which depends on genotypes, environmental factors and their interactions. Also, grain size is governed by the efficiency of assimilation and translocation of mineral nutrients (mainly nitrogen) during grain endosperm development, which affects grain yield. The aim of this study was to evaluate variability in the percentage of three different grain size classes: class I (thickness ³2.5 mm), class II (2.2-2.5 mm) and class III (<2.2 mm) in spring malting barley genotypes ('Novosadski 448', 'Novosadski 456', 'Dunavac' and 'Jadran'). The experiment was conducted during three years (2012-2014) in a randomized complete block design with three replications at different rates of nitrogen fertilization (N₁=45, N₂=75, N₃=105 and N₄=135 kg ha^-1). The presence of different grain sizes in barley cultivars in all N fertilization treatments after harvest was investigated. The proportion of the three grain classes was dependent upon year, cultivar and nitrogen fertilization rate. The highest percentage of class I grains was recorded in 'Novosadski 456', and that of class II and class III grains in 'Dunavac'. The percentage of class I grains increased significantly with increasing nitrogen rates up to 75 kg ha^-1, stagnated at 105 kg ha^-1, and decreased significantly as the nitrogen level was further increased to 135 kg ha^-1. Class II and class III grain contents decreased at nitrogen rates up to 105 kg ha^-1, but increased significantly at 135 kg ha^-1. The best response to favorable environmental conditions and the highest percentage of class I grain in all years were recorded in ‘Novosadski 456’. The most favorable effect on grain size in the studied spring malting barley genotypes was exhibited by the nitrogen rate of 75 kg ha^-1.

Keywords: barley, genotype, grain size, nitrogen, variability

Corresponding author: Aleksandar Paunović, University of Kragujevac, Faculty of Agronomy, Čačak, Serbia, Cara Dušana 34, 32000, Čačak, Serbia, Phone:+381 32 303 416, e-mail:aco@kg.ac.rs

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp. 1105-1114

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1703105M

Original scientific paper

ASSOCIATIONS OF POLYMORPHISMS IN THE PROLACTIN RECEPTOR GENE WITH GROWTH TRAIT IN JAPANESE QUAIL (Coturnix coturnix japonica)

Oreynab Looch MALEKI^1*, Ali HASHEMI¹, Ghorban Elyasi ZARRINGHABAIE²,

Mohammad FARHADIAN^3*

¹Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran

²Scientific member of Agriculture and Natural resources Research Center of East Azarbaijan Province, Tabriz, Iran

³Young Researchers and Elite Club, Khoy Branch, Islamic Azad University, Khoy, Iran

Abstract

Maleki L. O., A. Hashemi, G. E. Zarringhabaie, M. Farhadian (2017): Associations of polymorphisms in the prolactin receptor gene with growth trait in Japanese quail (Coturnix coturnix japonica).- Genetika, Vol 49, No.3, 1105-1114.

Prolactin receptor (PRLR), as an important regulatory gene about growth and differentiation, might be a condition gene for reproductive traits. The prolactin receptor is a specific receptor for prolactin (PRL), which is an anterior pituitary peptide hormone involved in various physiological activities and is essential for reproductive improvement. In chickens, the PRLR gene is on the Z chromosome. In the present study, the polymorphism of exon 2 of PRLR gene in Japanese Quail (Coturnix coturnixjaponica) was investigated. Blood samples of 180 Japanese Quail were collected randomly. DNA was extracted from blood samples and amplified. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of the exon 2 of PRLR gene. PCR-SSCP analysis of the exon 2 region revealed two banding patterns. Two different SSCP patterns, representing two different genotypes, were identified. The frequencies of the observed genotypes were 0.939 and 0.061 for AA and BB. Allele frequencies were 0.939 and 0.061 for A and B. Observed heterozygosity (Hobs) value was 0.0. The chi-square test showed significant (P<0.05) deviation from Hardy-Weinberg equilibrium for this locus in studied population. The effect of these genotypes on weight gain was investigated, and the BB genotype was found to be associated with body weight at 46 days. This was the first study on polymorphism of PRLR gene in Japanese quail

Key words: Genetic Polymorphism, Japanese Quail, PRL, SSCP

Corresponding author: Mohammad Farhadian, Young Researchers and Elite Club, Khoy Branch, Islamic Azad University, Khoy, Iran, Tel: 0989149765639, E-mail: Mohammad.farhadian@tabrizu.ac.ir

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp.1115-1126

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR17031115R

Original scientific paper

GENE EXPRESSION IN RESPONSE TO COTTON LEAF CURL VIRUS INFECTION IN Gossypium hirsutum UNDER VARIABLE ENVIRONMENTAL CONDITIONS

Iqra REHMAN¹, Beenish AFTAB¹, Muhammad BILAL SARWAR¹, *Bushra RASHID¹, Qurban ALI¹*, Muhammad Umair Majid¹, Sameera HASSAN¹, Muhammad AZAM ALI¹, Idrees AHMAD NASIR¹, Muhammad SALEEM HAIDER² and Tayyab HUSNAIN¹

¹Center of Excellence in Molecular Biology, University of the Punjab,Thokar Niaz Baig Lahore, Pakistan

²Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan

Abstract

Rehman I., B. Aftab, M. Bilal Sarwar, B. Rashid, Q. Ali, M. U. Majid, S. Hassan, M. Azam Ali, I. Ahmad Nasir, M. Saleem Haider and T. Husnain (2017): Gene expression in response to cotton leaf curl virus infection in Gossypium hirsutum under variable environmental conditions.- Genetika, Vol 49, No.3, 1115-1126.

Cotton Leaf Curl Disease (CLCuD) is one of the threatening constrains of cotton production in Pakistan for which no adequate remedy is available until now. Local variety of Gossypium hirsutum (FH-142) was grown in field and infected naturally by CLCuV under variable range of temperature and humidity. Plants showed thickening of veins in lower leaf surface at 34°C and 60% relative humidity at 15days post infection (dpi) and curling of leaf margins at 33°C with 58% relative humidity at 30dpi. Remarkable leaf darkening was observed with reduced boll formation at 45dpi at 26°C and 41% relative humidity. Enation developed, severe thickening and curling of leaves intensified and plants showed dwarf growth at 60dpi at 24°C with 52% relative humidity. PCR amplification of Rep associated gene confirmed the presence of CLCuD-associated begomovirus in the infected samples. Quantitative RT-PCR confirmed the amplification and differential expression of a number of pathogen stress responsive genes at different levels of temperature and humidity. This observation predicts that Cotton Leaf Curl Virus (CLCuV) interacts with several host genes that are upregulated to make plants susceptible or suppress other genes to overcome host defense responses.

Keywords: Cotton leaf curl virus, gene expression, Gossypium hirsutum, molecular analysis, plant pathogen

Corresponding author: Qurban Ali, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Thokar Niaz Baig Lahore 53700 – Pakistan, saim1692@gmail.com, bushra.cemb@pu.edu.pk

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Published in „GENETIKA“ Vol. 49, No.3 (2017), pp.1127

Serbia

Genetika, 49(3), pp. 1127, 2017

https://doi.org/10.2298/GENSR1703127E

CORRIGENDUM

Surma M., T. Adamski, Z. Kaczmarek, W. Święcicki, S. Mejza, P. Barzyk, A. Kuczyńska, K. Krystkowiak, K. Mikołajaczak, P. Ogrodowicz (2017): A multivariate approach to the selection of pea (Pisum sativum L.) lines obtained by the single seed descent technique.- Genetika, Vol 49, No.1., 365-376.

https://doi.org/10.2298/GENSR1701365S

The authors of the above-referenced article have requested a corrigendum to it. The article should include the following correction in name of author: insteed Krzysztof MIKOŁAJACZAK correct name is Krzysztof MIKOŁAJCZAK.

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