GENETIKA, Vol

GENETIKA, Vol. 50, No. 1(2018)

Vesna JANKOVIĆ, Savo VUČKOVIĆ, Vojislav MIHAILOVIĆ, Vera POPOVIĆ, Ljubiša ŽIVANOVIĆ, Divna SIMIĆ, Ana VUJOŠEVIĆ, Petar STEVANOVIĆ

ASSESSMENT OF SOME PARAMETERS PRODUCTIVITY AND QUALITY OF POPULATIONS Phleum pratense (L.) GROWN IN CONDITIONS OF SERBIA [Abstract] [Full text]

Ponnaiah GOVINTHARAJ, Swaminathan MANONMANI and Sabariappan ROBIN

CHARACTER ASSOCIATION AND PATH ANALYSIS IN BACTERIAL BLIGHT RESISTANCE GENES PYRAMIDED SEGREGATING POPULATIONS OF RICE (Oryza sativa L.)[Abstract] [Full text]

Hafiz Saad Bin MUSTAFA, Ejaz-ul-HASAN, Tariq MAHMOOD, Amir HAMEED and Qurban ALI

ENHANCING FOOD SECURITY IN ARID AREAS OF PAKISTAN THROUGH NEWLY DEVELOPED DROUGHT TOLERANT AND SHORT DURATION MUSTARD (Brassica juncea L.) CANOLA [Abstract] [Full text]

Mahdi bayat, Reza amirnia, Hakan özkan, Aysun gedik, Duygu ates, Bahattin tanyulac, Mehdi rahimi

DIVERSITY AND PHYLOGENY OF SAFFRON (Crocus sativus L.) ACCESSIONS BASED ON IPBS MARKERS [Abstract] [Full text]

Željko Lakić, Igor Balalić, Miloš Nožinić

Genetic variability for yield and yield components in Jerusalem artichoke (Helianthus tuberosus L.) [Abstract] [Full text]

Jalal rezaei, Mahboobeh zare mehrjerdi,Hassan mastali

ISSR BASED ANALYSIS OF GENETIC DIVERSITY IN SOME ENDANGERED SPECIES OF Allium subg. Melanocrommyum [Abstract] [Full text]

Biljana nikolić, Dragan kovačević, Snežana mladenović drinić, Ana nikolić, Zorica S. mitić, Srđan bojović, and Petar D. marin

RELATIONSHIPS AMONG SOME PINES FROM SUBGENERA Pinus AND Strobus REVEALED BY NUCLEAR EST-MICROSATELLITES [Abstract] [Full text]

Andrej šekularac, Aleksandra torbica, Dragan živančev, Jelena tomić, Desimir knežević

THE INFLUENCE OF WHEAT GENOTYPE AND ENVIRONMENTAL FACTORS ON GLUTEN INDEX AND THE POSSIBILITY OF ITS USE AS BREAD QUALITY PREDICTOR [Abstract] [Full text]

Sapna THAKUR, N B SINGH, Sanjeev THAKUR, J P SHARMA, R K GUPTA, M SANKANUR and S S BHAT

LINE × TESTER ANALYSIS FOR GROWTH AND BIOMASS CHARACTERISTICS OF SALIX [Abstract] [Full text]

Adriana F. sestraş, Lorentz jäntschi,Sorana D. bolboacă

USING THE GRIFFING’S EXPERIMENTAL DESIGN METHOD I, MODEL II. APPLE BREEDING – A CASE STUDY AS A PROPOSED METHODOLOGY OF THE STATISTICAL AND GENETIC ANALYSIS [Abstract] [Full text]

Dario DANOJEVIĆ and Slađana MEDIĆ-PAP

DIFFERENT MULTIVARIATE ANALYSIS FOR FRUIT TRAITS IN SWEET PEPPER BREEDING [Abstract] [Full text]

Marzeih SALEHI, Ahmad ARZANI, Majid TALEBI, and Asad ROKHZADI

GENETIC DIVERSITY OF WHEAT WILD RELATIVES USING SSR MARKERS [Abstract] [Full text]

Milica nikolic, Ana nikoliC, Marko jaukovic, Iva savic, Tanja petrovic, Ferenc bagi, Slavica stankovic

DIFFERENTIATION BETWEEN Aspergillus flavus AND Aspergillus parasiticus isolates originated FROM WHEAT [Abstract] [Full text]

Jaskanwal SINGH and R.K. DHALL

GENETIC VARIABILITY PARAMETERS OF YIELD AND QUALITY ATTRIBUTES IN VEGETABLE PEA (Pisum sativum L.) [Abstract] [Full text]

Jawad ALI SHAH, Muhammad MUJTABA, Sohail AHMAD JAN, Sultan AKBAR JADOON, Malik ASHIQ RABBANI, Abdul GHAFOOR, Muhammad JAHANZAIB and Haris KHURSHID

ESTIMATION OF SPATIAL GENETIC STRUCTURE IN INTER-REGIONAL POPULATIONS OF Trigonella foenum-graceum L. SPECIES THROUGH PHENOTYPIC VARIATION AND SEED PROTEIN PROFILING [Abstract] [Full text]

Milan JOCKOVIĆ, Siniša JOCIĆ, Slaven PRODANOVIĆ, Sandra CVEJIĆ, Mihajlo ĆIRIĆ, Petar ČANAK, Ana MARJANOVIĆ-JEROMELA

EVALUATION OF COMBINING ABILITY AND GENETIC COMPONENTS IN SUNFLOWER[Abstract] [Full text]

RAMANDEEP, T.S. DHILLON, R.K. DHALL, B.S. GILL

EFFECT OF MUTAGEN-ETHYL METHANE SULPHONATE ON YIELD INCREASING PARAMETERS OF FRENCH BEAN (Phaseolus vulgaris L.) [Abstract] [Full text]

Umakanta SARKER, Md. Tofazzal ISLAM, Md. Golam RABBANI, Shinya OBA

ANTIOXIDANT LEAF PIGMENTS AND VARIABILITY IN VEGETABLE AMARANTH [Abstract] [Full text]

Slavica PAPIĆ, Roman LONGAUER, Ivan MILENKOVIĆ, Jiří ROZSYPÁLEK

GENETIC PREDISPOSITIONS OF COMMON ASH TO THE ASH DIEBACK CAUSED BY ASH DIEBACK FUNGUS [Abstract] [Full text]

Hamid hatami maleki, Kiomars rouhrazi, Gholam khodakaramian, Naser sabaghnia

CHARACTERIZATION AND MOLECULAR DIVERSITY OF IRANIAN RHIZOBIA ISOLATED FROM FABA BEAN [Abstract] [Full text]

Marina LAZAREVIĆ, Dragan STANOJEVIĆ, Vladan BOGDANOVIĆ, Vlada PANTELIĆ, Nevena MAKSIMOVIĆ, Miloš MARINKOVIĆ, Nenad MIĆIĆ

VARIABILITY AND HERITABILITY OF MILK TRAITS OF HOLSTEIN - FRISIAN BULL DAMS AND THEIR PROGENY [Abstract] [Full text]

Biljana ROGIĆ, Božo VAŽIĆ, Đorđe SARAJLIĆ

BREEDING GOALS AND SELECTION EFFORT IN THE BREEDING OF LIPIZZAN HORSES IN THE STUD FARM VUČIJAKA FROM 1946 TO 2015 [Abstract] [Full text]

Valentin kosev and Viliana vasileva

Ecologo-genetical model for control of the quantitative traits in white lupin [Abstract] [Full text]

H. baneh and J. ahmadpanah

GENETIC EVALUATION OF BODY WEIGHT TRAITS IN IRANIAN NATIVE GHEZEL SHEEP [Abstract] [Full text]

Mirjana jovović, Vesna tunguz, Milan mirosavljević,Novo pržulj

EFFECT OF SALINITY AND DROUGHT STRESS ON GERMINATION AND EARLY SEEDLINGS GROWTH OF BREAD WHEAT (Triticum aestivum L.) [Abstract] [Full text]

Zoran CAMDZIJA, Vesna DRAGICEVIC, Jelena VANCETOVIC, Milan STEVANOVIC, Jovan PAVLOV, Milomir FILIPOVIC and Dragana IGNJATOVIC-MICIC

INHERITANCE OF INORGANIC AND PHYTIC PHOSPHORUS IN MAIZE (Zea mays L.) KERNEL [Abstract] [Full text]

Sarah AGGAG and Karim EL-SABROUT

POLYMORPHISM OF THE MELANOCORTIN RECEPTOR GENE AND ITS ASSOCIATION WITH EGG PRODUCTION TRAITS IN LOHMANN BROWN CHICKENS [Abstract] [Full text]

Mehmet Ali SÖZEN, Yasemin ÇETİNKAYA, Gizem GEÇGEL, Nuriye ÖZCAN, Ersin ÜLGER

INVESTIGATION OF ASSOCIATION BETWEEN THE HUMAN NEUROPEPTIDE Y GENE LEUCINE7PROLINE POLYMORPHIM AND OBESITY IN A POPULATION FROM TURKEY [Abstract] [Full text]

Daisuke watanabe, Tomáš lošáK, Johann vollmann

From proteomics to ionomics: Soybean genetic improvement for better food safety [Abstract] [Full text]

Júlia HUNKOVÁ, Gabriela LIBIAKOVÁ, Jozef FEJÉR, Tatjana VUJOVIĆ, Alena GAJDOŠOVÁ

TESTING OF DIFFERENT IRON SOURCES AND CONCENTRATIONS on shoot MULTIPLICATION OF blackberry (Rubus fruticosus L.) [Abstract] [Full text]

Corrigendum:

vojvodić S., D. ademović-sazdanić (2017): Impact of Hla-B*27 subtypes with increased susceptibility to seronegative spondyloarthropathies in Vojvodina population.- Genetika, Vol 49, No.3, 801- 808. [Abstract] [Full text]

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 1-10

UDC 575.630

https://doi.org/10.2298/GENSR1801001J

Orginal scientific paper

ASSESSMENT OF SOME PARAMETERS PRODUCTIVITY AND QUALITY OF POPULATIONS Phleum pratense (L.) GROWN IN CONDITIONS OF SERBIA

Vesna JANKOVIĆ ^1*, Savo VUČKOVIĆ¹, Vojislav MIHAILOVIĆ², Vera POPOVIĆ^2*,

Ljubiša ŽIVANOVIĆ¹, Divna SIMIĆ¹, Ana VUJOŠEVIĆ¹, Petar STEVANOVIĆ³

¹University of Belgrade, Faculty of Agriculture, Zemun–Belgrade, Serbia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Inspection Affairs Administration of Republic Srpska, Banja Luka, Bosnia&Herzegovina

Abstract

Janković V., S. Vučković, V. Mihailović, V. Popović, Lj. Živanović, D. Simić, A. Vujošević_,P. Stevanović (2018): Assessment of some parameters productivity and quality of populations Phleum pratense (L.) grown in conditions of Serbia.- Genetika, Vol. 50, No. 1, 1-10.

Phleum pratense (L.) is the important forage grass species in Serbia. The study was conducted on 20 autochthonous population of Phleum pratense (L.), originating from Western Serbia. Plant height, protein content and crude cellulose content had a high direct impact on yield. Populations of PP16, PP20, PP2 and PP9 had the significantly higher yield of the raw matter compared to all the tested population and the highest values for the other evaluated parameters, also. The average yield of crude biomass was positive statistically highly significantly correlated with plant height (r=0.87**), and positively significantly correlated with a content of crude proteins and positive non significant correlated with crude cellulose (r=0.42^ns). Based on obtained values and by the appropriate choice of selection methods, we conclude that we have excellent genotypes, PP16, PP20, PP2 and PP9 for a successful selection process in order to obtain new high yielding varieties of Phleum pratense.

Key words: Timothy grass, population, yield components, chemical composition, correlation

Corresponding author: Vesna Janković, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Zemun–Belgrade, Serbia and Dr Vera Popović, Institute of Field and Vegetable Crops, Novi Sad, Serbia, E- mail: bravera@eunet.rs; vjjankovic@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp.11-20

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801011G

Original scientific paper

CHARACTER ASSOCIATION AND PATH ANALYSIS IN BACTERIAL BLIGHT RESISTANCE GENES PYRAMIDED SEGREGATING POPULATIONS OF RICE

(Oryza sativa L.)

Ponnaiah GOVINTHARAJ*, Swaminathan MANONMANI and Sabariappan ROBIN

Department of Rice, Centre for Plant Breeding and Genetics,

Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India

Abstract

Govintharaj P., S. Manonmani, and S. Robin (2018): Character association and path analysis in bacterial blight resistance genes pyramided segregating populations of rice (Oryza sativa L.).- Genetika, Vol 50, No.1, 11-20.

An exploratory research work was conducted to study the character association and their direct and indirect effects on grain yield in three bacterial blight resistance genes introgressed segregating populations (F₂) in rice. Data for yield and five other traits which were component of yield were recorded in 449 F₂individuals in all three crosses. Thousand grain weight was highly correlated with single plant yield in (r=0.95**, p<0.01) CB 174 R × IRBB 60> (r=0.48**, p<0.01) CB 87 R × IRBB 60> (r=0.44**, p<0.01) TNAU CMS 2B × IRBB 60. Thousand grain weight was exhibited important yield determining component for CB 174 R × IRBB 60 (0.92) and CB 87 R × IRBB 60 (0.45), and panicle length for (0.39) TNAU CMS 2B × IRBB 60 by direct effect. The genetic information derived from our study could be useful to select potential segregating individual among the F₂ population for future rice breeding program.

Key words: early generation, correlation coefficient, direct and indirect effects, yield components

Corresponding author: Ponnaiah Govintharaj, Department of Rice, Centre for Plant Breeding and Genetics,Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India, govinth.tnau@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp.21-31

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801021M

Original scientific paper

ENHANCING FOOD SECURITY IN ARID AREAS OF PAKISTAN THROUGH NEWLY DEVELOPED DROUGHT TOLERANT AND SHORT DURATION MUSTARD (Brassica juncea L.) CANOLA

Hafiz Saad Bin MUSTAFA¹*, Ejaz-ul-HASAN¹, Tariq MAHMOOD¹, Amir HAMEED²

and Qurban ALI³

¹Directorate of Oilseeds, Ayub Agricultural Research Institute, Faisalabad, Pakistan

²Statistical Section, Ayub Agricultural Research Institute, Faisalabad, Pakistan

³Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan

Abstract

Mustafa H. S. B., E.-ul-Hasan, T. Mahmood, A. Hameed and Q. Ali (2018): Enhancing food security in arid areas of Pakistan through newly developed drought tolerant and short duration mustard (Brassica juncea L.) canola .- Genetika, Vol 50, No.1, 21-31.

Food security is the crucial global issue, especially in developing countries like Pakistan. Since edible oil is an essential food item, its persistent paucity in the country and huge import for meeting domestic requirements, has attained it second largest import item after petroleum products. The aim of present study is qualitative and quantitative evaluation of newly developed short duration and drought tolerant canola quality Brassica juncea lines ZBJ-06012 and ZBJ-08051 to overcome the unfavorable edible oil situation in the country. Thirteen lines were evaluated in randomized complete block design (RCBD) for seed yield, oil quality, maturity period and drought tolerance under different agro-climatic zones both in irrigated and arid areas across the Punjab province at eight locations in Micro Yield Trials during Rabi season 2012-13 and 2013-14. Presently, grown non-canola mustard varieties Khanpur Raya and Anmol Raya were used as check varieties. Brassica napus cultivars Punjab canola and Faisal canola were also included in the trials for comparison study of mustard and rapeseed genotypes. Data for all traits under observation was analyzed through Principle Component Analysis (PCA) to evaluate the best performing lines in irrigated as well as in rain fed areas. Principal Component Analysis showed first 2 PCs having Eigen value >1 explaining 76.4% and 72% of the total variation at irrigated areas and rain fed areas respectively. The mean seed yield was also compared by Least Significant Difference (LSD) test to study the significance at 5% probability level. Canola quality B. juncea lines ZBJ-06012 and ZBJ-08051 have shown good adaptability, early maturity, non-shattering, disease and drought tolerance traits with high yield potential in comparison with presently grown Brassica napus cultivars “Punjab Canola” and “Faisal Canola”. Due to these prominent features, these lines have a great scope for motivating farmers to grow canola quality B. juncea when compared with B. napus and non-canola B. juncea. Future challenges demand further development of high yielding, short duration and aphid tolerant mustard cultivars having high oil content and canola quality. There is a great potential of exploiting genetic variability in the existing B. juncea material to achieve the aforesaid goals by using conventional plant breeding techniques.

Key words: B. juncea, drought tolerance, food security, genetic variability, short duration

Corresponding author: Hafiz Saad Bin Mustafa, Directorate of Oilseeds, Ayub Agricultural Research Institute, Faisalabad, Pakistan, Email: saadpbg@gmail.com, phone: +92-41-9200770

Return to content

Published in „GENETIKA“ Vol. 50, No1 (2018), pp. 33-44

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801033B

Original scientific paper

DIVERSITY AND PHYLOGENY OF SAFFRON (Crocus sativus L.) ACCESSIONS BASED ON IPBS MARKERS

Mahdi bayat¹, Reza amirnia², Hakan özkan³, Aysun gedik³, Duygu ates³, Bahattin tanyulac³, Mehdi rahimi⁴

¹Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran. ²Faculty of Agriculture, Urmia University, Urmia, Iran.

³Department of Bioengineering, EBILTEM institute, EGE University, Izmir, Turkey.

⁴ Assistant prof. of Biotechnology, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

Abstract

bayat M., R. amirnia, H. özkan, A. gedik, D. ates, B. tanyulac, M. rahimi (2018): Diversity and phylogeny of saffron (Crocus sativus L.) accessions based on IPBS markers.- Genetika, Vol 50, No.1, 33-44.

This study sought to measure genetic diversity and phylogenetic structure among 196 individuals of saffron from 20 geographically separate accessions from Iran, Spain and Turkey using retrotransposon derived iPBS markers. Twenty-eight primers amplified a total of 179 polymorphic alleles with an average of 6.4 bands per primer. The average of parameters shannon's information index, genetic distance and gene diversity was 0.483, 0.286 and 0.841 respectively. Polymorphic information index ranged from 0.407 to 0.953 with an average of 0.824. Primers 2298, 2229 and 2393 with 0.953, 0.943 and 0.943 PIC respectively, identified as the most informative primers in this study. The results of phylogenetic trees showed that twenty saffron accessions were placed into four major clusters that matched with their geographical locations completely. These results are supported by principal coordinate analysis. Overall, we can confirm that iPBS markers as low cost and high efficient molecular markers are a powerful DNA fingerprinting for assessing genetic diversity and phylogenetic analysis among saffron accessions originating from different geographical regions.

Key words: Cluster analysis, diversity, inter-primer binding site (iPBS), molecular markers, retrotransposon, saffron (Crocus sativus L.).

Corresponding author: Mehdi Rahimi, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran, E-mail: mehdi83ra@yahoo.com

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp. 45-57

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801045L

Original scientific paper

Genetic variability for yield and yield components in Jerusalem artichoke (Helianthus tuberosus L.)

Željko Lakić¹, Igor Balalić², Miloš Nožinić¹

¹Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia and Herzegovina

²Institute of Field and Vegetable Crops, Department for oil crops, Novi Sad, Serbia

Abstract

Lakić Ž., I. Balalić, M. Nožinić (2018): Genetic variability for yield and yield components in jerusalem artichoke (Helianthus tuberosus L.).- Genetika, Vol 50, No.1, 45-57.

Investigation of quantitative traits of Jerusalem artichoke populations was conducted on the experimental field of the Agricultural Institute of the Republic of Srpska in Banja Luka, during 2013 and 2014. The material was collected in the wider area of Republic of Srpska. The following populations were analyzed: Srbac, Modriča, Pivara, Lazarevo, Bosna, Vrbas, Gradiška and Aleksići. The trial was arranged in a randomized complete block design with three replications. The following parameters were analyzed: tuber weight (g), plant height (cm), stem number/plant, average stem thickness (cm), green mass yield/plant (kg), and dry mass yield/plant (kg). Average tuber weight ranged from 36.7 g (Bosna) to 61.0 g (Lazarevo). Plant height ranged from 1.55 m (Lazarevo) to 2.20 m (Aleksići). Stem number/plant varied from 2.2 (Aleksici) to 5.3 (Modrica). Stem thicknesswas between 1.24 cm (Bosna) and 2.11 cm (Aleksići). The population Bosna had the lowest average yield of green mass and dry mass yield/plant, while the highest mean value had population Aleksići. Broad sense heritability varied from 34.66% (plant height) to 50.99% (dry mass yield/plant). Highly significant positive correlations between plant height and stem thickness (r = 0.874), stem thickness and green mass yield (r = 0.919), stem thickness and dry mass yield (r = 0.902) were established. Jerusalem artichoke populations were clustered into two groups, and the first group was composed of two sub-groups.The results of these studies will allow choice of Jerusalem artichoke populations with better quantitative traits, and their inclusion in the program of creating new varieties.

Keywords: correlation, heritability, interaction, quantitative traits, topinambour

Corresponding author: Željko Lakić, PI Agricultural Institute of Republic of Srpska, Knjaza Miloša 17, 78 000 Banja Luka, Republic of Srpska, BiH; Phone: +387 51 303 112, Fax: +387 51 312 792; E-mail: lakic.kiko@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 59-68

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1801059R

Original scientific paper

ISSR BASED ANALYSIS OF GENETIC DIVERSITY IN SOME ENDANGERED SPECIES OF Allium subg. Melanocrommyum

Jalal rezaei, Mahboobeh zare mehrjerdi^*,Hassan mastali

Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran

Abstract

rezaei J., M. zare mehrjerdi,H. mastali (2018): ISSR based analysis of genetic diversity in some endangered species of Allium subg. melanocrommyum.- Genetika, Vol 50, No.1, 59-68.

Melanocrommyum, a subgenus of the Allium genus, is found in different regions of Iran and is in danger of extinction due to excessive exploitation. This study aimed to determine the genetic diversity in 170 individuals representing 17 wild populations belonging to six endangered species of Allium subg. Melanocrommyum using inter simple sequence repeat (ISSR) markers. The 10 selected ISSR primers produced 178 polymorphic fragments (100%). Polymorphic band number varied from 12 (primer 8) to 22 (primer 2). The average observed number of alleles, effective number of alleles, Shannon’s indices and Nei’s gene diversity were 1.48, 1.2, 0.2 and 0.1, respectively. According to Nei’s genetic distance, the lowest genetic distance (0.048) was observed among both two populations of A. elburzense (Emamzadeh Ebrahim and Kamelat), and two populations of A. subakaka (Jame Shoran and Ghalelan) while the highest distance (0.097) was observed among a population of A. kurdistanicum (Taze Abad Oryeh) with both A. pseudobodeanum (Shen Jari), and A. derderianum (Dareh Oson) populations. In UPGMA cluster analysis, the populations were grouped into four main clusters at a cutoff value of 0.07. The analysis of molecular variance showed that the maximum value of genetic variation was found within the populations (68%), where as a low genetic differentiation was observed among the populations (32%). Our results revealed that ISSR molecular markers are useful to display the diversity in Allium genus and can be used to improve the classification accuracy. This study provided valuable information for the conservation of these species and breeding program planning.

Keywords: Allium, genetic diversity, ISSR, melanocrommyum, population

Corresponding author: M. Zare Mehrjerdi, Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran. E-mail: mzarem@ut.ac.ir. Tel:+982136041089

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp.69-84

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801069N

Original scientific paper

RELATIONSHIPS AMONG SOME PINES FROM SUBGENERA Pinus

AND Strobus REVEALED BY NUCLEAR EST-MICROSATELLITES

Biljana nikolić^1*, Dragan kovačević², Snežana mladenović drinić²,

Ana nikolić², Zorica S. mitić³, Srđan bojović⁴, and Petar D. marin⁵

^1*Institute of Forestry, Belgrade, Serbia

²Maize Research Institute “Zemun Polje”, Belgrade, Serbia,

³University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Niš, Serbia,

⁴University of Belgrade, Institute for Biological Research “Siniša Stanković” Belgrade, Serbia,

⁵University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade, Serbia

Abstract

nikolić B., D. kovačević, S. mladenović drinić, A. nikolić, Z. S. mitić, S. bojović, and P. D. marin (2018): Relationships among some pines from subgenera pinus and strobus revealed by nuclear EST-microsatellites.- Genetika, Vol 50, No.1, 69-84.

Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.

Key words: Bosnian pine, Macedonian pine, Austrian pine, nuclear EST-SSR markers

Corresponding author: Biljana nikolić, Institute of Forestry, Kneza Višeslava 3, 11000 Belgrade, Serbia, Corresponding author, E-mail: smikitis@gmail.com, phone: +381 62 8838009

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp.85-93

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1801085S

Original scientific paper

THE INFLUENCE OF WHEAT GENOTYPE AND ENVIRONMENTAL FACTORS

ON GLUTEN INDEX AND THE POSSIBILITY OF ITS USE AS BREAD QUALITY PREDICTOR

Andrej šekularac¹, Aleksandra torbica², Dragan živančev²,

Jelena tomić², Desimir knežević^1*

¹University of Prishtina, Faculty of Agriculture, Kosovska Mitrovica, Lešak, Kosovo

and Metohia, Serbia

²University of Novi Sad, Institute of Food Technology, Novi Sad, Serbia

Abstract

Šekularac, A., A. Torbica, D. Živančev, J. Tomić and D. Knežević (2018): The influence of wheat genotype and environmental factors on gluten index and the possibility of its use as bread quality predictor.- Genetika, Vol 50, No.1, 85-93.

Gluten index is an indicator of gluten strength as well as a parameter which simultaneously defines its quantity and quality. If compared to the farinographic and extensographic methods, gluten index determining is faster, less complicated and requires smaller amount of flour. The aim of this study has been to determine the significance of the influence of genotype and environmental factors on the value of gluten index and usability of these parameters as indicators and predictors of bread quality. Five local varieties of winter wheat (Simonida, NS 40S, Rapsodija, Pobeda, Zvezdana) were grown in a macro-experiment carried out in three locations (Novi Sad, Čačak, Sombor) during two seasons (2011/2012 and 2012/2013). Gluten index value determination was achieved by using ICC standard method 155 (1996), with a slight modification that included mechanical washing of gluten (Theby). Statistical analysis was performed by using IBM SPSS Statistics 20. Variance analysis revealed a statistically significant effect of genotype on gluten index value, whereas the influence of environmental factors, as well as the interaction of two factors, had no statistical significance. There was no correlation between the values of gluten index and meteorological factors such as average temperature and total precipitation during the phenophase of grain filling, and in the period from the beginning of flowering until the harvest. However, medium-strong negative dependence was found between gluten index and the index of heat stress, as well as between gluten index and the number of days with temperatures above 30°C. Weak negative dependence was recorded between gluten index and bread volume. Based on the value of gluten index, gluten varieties used in our study can be described as strong. Genotype proved to be the only cause of statistically significant variation of gluten index.

Key words: bread volume, environment, gluten, gluten index, genotype, wheat

Corresponding author: Desimir Knežević, University of Prishtina, Faculty of Agriculture, Kosovska Mitrovica, Lešak, Kosovo and Metohia, Serbia, Phone +381 64 614 88 82, e-mail:deskoa@ptt.rs

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp. 95-106

Serbia

UDC 57.630

https://doi.org/10.2298/GENSR1801095T

Original scientific paper

LINE × TESTER ANALYSIS FOR GROWTH AND BIOMASS CHARACTERISTICS OF SALIX

Sapna THAKUR¹, N B SINGH¹, Sanjeev THAKUR¹, J P SHARMA¹, R K GUPTA²,

M SANKANUR¹ and S S BHAT¹

¹Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni- Solan- 173 230, Himachal Pradesh

²Department of Basic Sciences, College of Forestry, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni- Solan- 173 230, Himachal Pradesh

Abstract

Thakur S., N B Singh, S. Thakur, J P Sharma, R K Gupta, M Sankanur, S S Bhat (2018): Line × tester analysis for growth and biomass characteristics of Salix.- Genetika, Vol 50, No.1, 95-106.

Line × tester analysis was carried out in willows (Salix spp.) to determine the genetic interaction in the expression of various quantitative characters related to productivity. Combining ability revealed that the estimates of GCA variance (σ² GCA) were more than the SCA variance (σ² SCA) for all the characters studied. Later the gene action study revealed that additive variance was observed more than the dominance variance for all the parameters studied. The proportional contribution of lines were higher than individual contribution of testers or line × tester interaction except for fresh root weight, dry shoot weight, dry root weight and total dry weight where the contribution of interactions was more. Line PN 227 and tester Austree and J 795 were found to be good general combiners and thus appeared to be worthy of exploiting in Salix improvement through breeding and recurrent selection followed by cloning for developing commercial superior clones. On the basis of mean performance and significant desirable SCA effects, the combinations PN 227 × Austree, PN 227 × NZ 1140 and J 799 × Austree were found to be the most promising families for growth and biomass characters and are recommended for within family selections followed by heterotic breeding.

Keywords: Biomass, combining ability, line, tester, Salix improvement

Corresponding author: Sapna Thakur,Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni- Solan- 173 230, Himachal Pradesh, email: sapnakullu_thakur@rediffmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 107-120

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801107S

Original scientific paper

USING THE GRIFFING’S EXPERIMENTAL DESIGN METHOD I, MODEL II. APPLE BREEDING – A CASE STUDY AS A PROPOSED METHODOLOGY OF THE STATISTICAL AND GENETIC ANALYSIS

Adriana F. sestraş¹, Lorentz jäntschi^2,3,Sorana D. bolboacă^4,5

¹University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Horticulture, Department of Forestry, Cluj-Napoca, Romania

²Technical University of Cluj-Napoca, Cluj-Napoca, Romania

³Babeş-Bolyai University, Doctoral Studies, Cluj-Napoca, Romania

⁴Iuliu Haţieganu University of Medicine and Pharmacy, Department of Medical Informatics and Biostatistics, Cluj-Napoca, Romania

⁵University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Doctoral Studies, Cluj-Napoca, Romania

Abstract

sestraş A.F., L. jäntschi, S.D. bolboacă (2018): Using the Griffing’s experimental design method I, model II. apple breeding – a case study as a proposed methodology of the statistical and genetic analysis.- Genetika, Vol 50, No.1, 107-120.

The diallel mating design is widely used in plant breeding to estimate combining ability of genitors and useful genetic parameters. In this work, a complete diallel mating design with four apple cultivars used as genitors was set, in order to elaborate a pattern for a quantitative trait, respectively the height of apple hybrids, measured at three months after emergence. Griffing’s method I, model II, with random effects, also known as complete diallel mating design, was included in a broader context of a methodology for both statistical and genetic analysis of the experimental data. An algorithm was developed based on the proposed methodology and tested on the experimental data as well as on other two simulated scenarios of complete diallel mating design with respectively three and seven parents fed with random values. The results have illustrated the pattern suitability for the quantitative inherited trait as was the height of the apple seedlings, respectively the speed of growth of the very young plants. In addition, the proposed algorithm can help young researchers to understand and use adequately for different similar traits the complexity of statistical and genetic analysis of the full diallel mating design. The information obtaining using diallel crosses offers the breeders the possibility to choose the appropriate breeding and selection strategies for quantitative traits.

Key words: algorithm, apple, genetic analysis, phenotypic characteristics, statistical analysis

Corresponding author: Lorentz Jäntschi, Babeş-Bolyai University, Doctoral Studies, 1st Mihail Kogălniceanu, 400028 Cluj-Napoca, Romania; lorentz.jantschi@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 121-129

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1801121D

Original scientific paper

DIFFERENT MULTIVARIATE ANALYSIS FOR FRUIT TRAITS

IN SWEET PEPPER BREEDING

Dario DANOJEVIĆ* and Slađana MEDIĆ-PAP

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Danojević D. and Medić-Pap (2018): Different multivariate analysis for fruit traits in sweet pepper breeding.- Genetika, Vol 50, No.1, 121-129.

Pepper is very popular vegetable crop in Southeast Europe and in Serbia as well. Wide genetic variability is essential in pepper breeding programs. The aim of this study was to evaluate variability for the most important fruit traits and differences between 28 sweet pepper genotypes from the working collection from the Institute of Field and Vegetable Crops (Novi Sad, Serbia). The following traits were analyzed: fruit weight (g), fruit length (cm), fruit diameter (cm), fruit index, number of locules, number of apexes, pericarp thickness (mm), and total soluble solids (°Brix). Results confirmed great variability in evaluated pepper fruit traits. Genotypes were separated into individual groups based on fruit characteristics. According to our research, hierarchical cluster analysis represented differentiation among groups of genotypes more clearly than PCA, but not comparing to k-means. Hierarchical cluster analysis showed the similarity between genotypes, but k-means clustering did not. Genotypes from group 3 will be used in breeding for higher fruit weight and group 6 for thicker pericarp.

Keywords: Capsicum annuum, genotype, fruit characteristic, cluster analysis, principal component analysis

Corresponding author: Dario Danojević, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: +381 21 4898 352, Fax: +381 21 4898 355, E-mail: dario.danojevic@ifvcns.ns.ac.rs

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp. 131-141

Serbia

UDC 575.630.11

https://doi.org/10.2298/GENSR1801131S

Original scientific paper

GENETIC DIVERSITY OF WHEAT WILD RELATIVES USING SSR MARKERS

Marzeih SALEHI¹, Ahmad ARZANI^1*, Majid TALEBI², and Asad ROKHZADI³

¹Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan-8415683111, Iran

²Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan-8415683111, Iran

³Agronomy Department, Islamic Azad University-Sanandaj Branch, Sanandaj, Iran.

Abstract

Salehi M., A. Arzani, M. Talebi, and A. Rokhzadi (2018): Genetic diversity of wheat wild relatives using SSR markers.- Genetika, Vol 50, No.1, 131-141.

Wild relatives of wheat are potential sources of valuable genetic materials for wheat improvement. Knowledge of the genetic diversity of wild relative species of wheat is crucial for their conservation and utilization. The objective of the current study was to investigate the genetic diversity of inter and intra species of Triticum monococcum ssp. aegilopoides (AA), Aegilops tauschii (DD) and Aegilops cylindrica (CCDD) originating from northern and western Iran. Thirty microsatellite (SSR) markers belonging to A, B, C and D genomes were used for analysis and 20 found to be polymorphic within and between species. The SSR markers generated a total number of 180 alleles with an average of 9 alleles per locus in 21 genotypes. The genetic diversity for all loci ranged from 0.74-0.90 with an average of 0.83. The highest genetic diversity was estimated for Xgwm186 and Xgwm205 which the latter could amplify in the A, D and CD genomes of T. monococcum, Ae. tauschii and Ae. cylindrica, respectively. In addition, the number of bands generated by Xgwm205 along with other four markers in Ae. cylindrica (CD) was two-fold than that of Ae. tauschii (D). Polymorphic information content ranged from 0.7-0.89 with an average of 0.82. The dendrogram obtained from the neighbor-joining method divided the genotypes of the three species into three distinctive groups. It can be concluded that SSR markers can be useful not only in differentiating wild species of wheat possessing A, D and C genomes, but also in assessing the genetic variation of genotypes within these species.

Keywords: Aegilops spp., Triticum monococcum, SSR markers, wild species

Corresponding author: Ahmad Arzani, Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan-8415683111 Iran, Tel. +98 31 33913453, Fax. +98 31 33912254, E-mail: a_arzani@cc.iut.ac.ir

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 143-152

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801143N

Original scientific paper

DIFFERENTIATION BETWEEN Aspergillus flavus AND Aspergillus parasiticus isolates originated FROM WHEAT

Milica nikolic¹, Ana nikoliC¹, Marko jaukovic², Iva savic¹,

Tanja petrovic¹, Ferenc bagi³, Slavica stankovic¹

¹Maize Research Institute, Zemun Polje, Belgrade, Serbia

²JV Jugoinspekt Beograd, Belgrade, Serbia

³Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

Abstract

nikolic M., A. nikolic, M. jaukovic, I. savic, T. petrovic, F. bagi, S. stankovic (2018): Differentiation between Aspergillus flavus and Aspergillus parasiticus isolates originated from wheat.- Genetika, Vol 50, No.1, 143-152.

The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia.

The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia.

This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.

Key words: aflatoxins, Aspergillus flavus, A. parasiticus, wheat

Corresponding author: Milica Nikolic, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia,E-mail: mnikolic@mrizp.rs phone number: +381648406456

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp. 153-170

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1801153S

Original scientific paper

GENETIC VARIABILITY PARAMETERS OF YIELD AND QUALITY ATTRIBUTES IN VEGETABLE PEA (Pisum sativum L.)

Jaskanwal SINGH and R.K. DHALL

Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, INDIA

Abstract

Singh J. and R.K. Dhall (2018): Genetic variability parameters of yield and quality attributes in vegetable pea (Pisum sativum L.).- Genetika, Vol 50, No.1,153-170.

A field experiment was conducted to study the genetic variability, heritability and genetic advance of 24 morphological traits and three biochemical traits in 47 genotypes of vegetable pea. The analysis of variance showed significant differences among genotypes for all the characters under study. Highest coefficient of variation was observed for number of podding nodes per plant and highest phenotypic and genotypic coefficients of variation were recorded for seed yield per plant followed by number of primary branches per plant and green pod yield per plant. Very high heritability estimates were observed for days to last pod picking (97.63) followed by plant height (97.40), days to first pod picking (96.78) and shelling percentage (95.56) while genetic advance as percentage of mean was found high for seed yield per plant (65.95) and primary branches per plant (58.40). High heritability along with moderate to high genetic advance was predicted in case of pod weight and number of seeds per pod, which indicated the role of additive gene action for the inheritance of these traits and therefore, selection could be used for improving these traits.

Key words: genetic advance, heritability, quality, variability, vegetable pea, yield

Corresponding author: R.K.Dhall, Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, Punjab, India. Ph (M): +91-8283840078, E-mail: rajinderkumar@pau.edu

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 171-185

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801171S

Original scientific paper

ESTIMATION OF SPATIAL GENETIC STRUCTURE IN INTER-REGIONAL POPULATIONS OF Trigonella foenum-graceum L. SPECIES THROUGH PHENOTYPIC VARIATION AND SEED PROTEIN PROFILING

Jawad ALI SHAH¹, Muhammad MUJTABA^1,2, Sohail AHMAD JAN^{3, 5}, Sultan AKBAR JADOON¹, Malik ASHIQ RABBANI³, Abdul GHAFOOR³, Muhammad JAHANZAIB⁴

and Haris KHURSHID^4*

¹ Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Swabi, Pakistan

²Institute of Biotechnology, Ankara University, Ankara 06110, Turkey

³ Plant Genetic Resources Institute, National Agricultural Research Centre, Islamabad, Pakistan

⁴Oilseeds Research Program, National Agricultural Research Centre, Islamabad, Pakistan

⁵Centre of Biotechnology & Microbiology, University of Swat, Swat, KP, Pakistan

Abstract

Shah Ali J., M. Mujtaba, S. Ahmad Jan, S. Akbar Jadoon, M. Ashiq Rabbani, A. Ghafoor, M. Jahanzaiband H. Khurshid (2018): Estimation of spatial genetic structure in inter-regional populations of Trigonella foenum-graceum L. species through phenotypic variation and seed protein profiling.- Genetika, Vol 50, No.1, 171-185.

Fenugreek (Trigonella foenum-graceum L.) is an important legume crop mainly grown for its pharmacological and nutritional value in Mediterranean region, western Asia, Indian sub-continent and Africa. We evaluated 110 fenugreek accessions from diverse agro-ecological regions i.e. South Asia, Mediterranean, Middle East, Europe and Africa for phenotypic divergence and seed protein based variation. Significant agro-morphological variability was revealed by germplasm viz-a`-viz traits e.g days to flower initiation, days to flower completion, yield plant^-1, plant habit, vigor, flower colour and plant height. Multivariate approach of Principal Component Analysis and Euclidean distance generated dendrogram distributed all accessions into 6 and 9 distinct groups for morpho-agronomic dissimilarities, respectively. Four principal components (PCs) with Eigen value higher than unity (E>1), represented 65% variability in germplasm. Geographical distribution was evident by scatter plot as germplasm figured in 6 different sub-populations. Iranian accessions were most diverse, showing up in all sub-populations followed by Indian, Turkish, Ethiopian, Pakistani and Egyptian accessions which ranked in 5,4,4,3 and 3 sub-populations, respectively. Electrophoretic pattern of seed protein also exhibited considerable polymorphism in the range of 30~100 kDa. Maximum of 16 bands were produced in Turkish PI171872 and Indian PI175321 genotypes. UPGMA based cluster analysis distributed all accessions in 5 groups where accessions from close geographical proximity settled adjacently.

Key words: Fenugreek, genetic diversity, GIS, spatial heterogeneity

Corresponding author: Haris Khurshid, Oilseeds Research Program, National Agricultural Research Centre, Islamabad, Pakistan. Email: hariskhurshid8@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 187-198

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801187J

Original scientific paper

EVALUATION OF COMBINING ABILITY AND GENETIC COMPONENTS

IN SUNFLOWER

Milan JOCKOVIĆ¹, Siniša JOCIĆ¹, Slaven PRODANOVIĆ², Sandra CVEJIĆ¹,

Mihajlo ĆIRIĆ¹, Petar ČANAK¹, Ana MARJANOVIĆ-JEROMELA¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agriculture, Belgrade University, Zemun, Serbia

Abstract

Jocković M., S. Jocić, S. Prodanović, S. Cvejić, M. Ćirić, P. Čanak, A. Marjanović-Jeromela (2018): Evaluation of combining ability and genetic components in sunflower.- Genetika, Vol 50, No.1, 187-198.

Evaluation of genetic material involved in breeding proces is of great importance in order to choose the most valuable genotypes with ability to efficiently combine desirable genes in progeny. The objective of this study was to utilize diallel method in order to estimate mode of inheritance, heterosis effect, combining abilities and components of genetic variance for important properties in sunflower and to identify those genotypes that can make progress in sunflower breeding. Present research had been carried out during 2012 and 2013 growing seasons. Evaluation included 6 sunflower genotypes and their 15 combinations which were crossed according to incomplete diallel. For seed yield/plant dominance of better parent (d+) was expressed as a mode of inheritance, while regarding thousand seed weight the most common modes of inheritance were dominance (d+) and partial dominance of better parent (pd+). The most common mode of inheritance regarding oil content was superdominance (sd+). Empirical estimates of combining abilities of genotypes involved in breeding process is main startegy in evaluation of breeding value. Regarding seed yield/plant significant and positive heterosis effect (28.9%) was calculated in combination Harkovski x Rodnik (P3xP5), while the highest heterosis effect for oil content (12.8%) was determined in combination Harkovski x Amaian (P3xP6). Combining ability analysis indicated that both genetic components, additive and non-additive, were important in expression of investigated traits. Components of genetic variance indicated prevalence of dominant component (H) compared to additive (D) and higher concentration of dominant genes (u) compared to recessive (v), in all investigated traits.

Key words: sunflower, mode of inheritance, heterosis, combining abilities, genetic components

Corresponding author: Milan Jocković, Institute of Field and Vegetable Crops, Novi Sad, Serbia, e-mail: milan.jockovic@ifvcns.ns.ac.rs; jockovic@gmail.com,Tel: 0214898303

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp.199-207

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801199R

Original scientific paper

EFFECT OF MUTAGEN- ETHYL METHANE SULPHONATE ON YIELD INCREASING PARAMETERS OF FRENCH BEAN (Phaseolus vulgaris L.)

RAMANDEEP, T.S. DHILLON, R.K. DHALL, B.S. GILL

Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, India

Abstract

Ramandeep, T.S. Dhillon, R.K. Dhall, B.S. Gill (2018): Effect of mutagen-ethyl methane sulphonate on yield increasing parameters of French bean (Phaseolus vulgaris L.).- Genetika, Vol 50, No.1, 199-207.

The present investigation was undertaken at the Vegetable Research Farm, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, INDIA during spring season of 2015 and 2016 with objective to induce genetic variability for yield and quality traits in cultivars ‘Contender’ and ‘Arka Komal’ through chemical mutagen- ethyl methane sulphonate (EMS) at 0.15, 0.25 and 0.35%. Seed treatment with all the EMS concentration drastically reduced its germination but the germination per cent decreased with increase in concentration of EMS. The per cent seed germination was maximum (90%) in control ‘Contender’ whereas it was 74%, 49% and 42% in EMS at 0.15%, 0.25% and 0.35%, respectively. Similar trend of results for germination percentage were obtained in cultivar ‘Arka Komal’ after treatment with different concentrations of EMS, however germination percentage of ‘Arka Komal’ was lower than of ‘Contender’. The germination percentage trends of EMS treated French bean cultivars were comparable under field and laboratory conditions but somewhat less under field due to less favourable conditions. All the plants harvested in M₁ generation in both cultivars were sown in plant to progeny fashion along with parents. Several desirable plants were selected in M₂generation for early flowering, number of pods per plant, pod length and bean common mosaic virus (BCMV) resistance in both the cultivars at different concentrations of EMS. Selection was also performed for straight pods in case of genotype 'Contender'. The maximum frequency of desirable mutants was observed in lower EMS concentration (0.15%). The 0.15% EMS increased number of pods per plant which may be directly related to yield per plant and it will ultimately increase the total yield. Therefore, EMS concentration at 0.15% can be effectively used for inducing maximum variability in French bean. The mutant population developed in M₂generation can be further used to check its performance in next generations.

Key words: Bean common mosaic virus, French bean, Mutation, Pod shape, Variability, Yield

Corresponding author: R.K. Dhall, Department of Vegetable Science, Punjab Agricultural University, Ludhiana – 141001, Punjab, INDIA. E-mail: rajinderkumar@pau.edu

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 209-220

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1801209S

Original scientific paper

ANTIOXIDANT LEAF PIGMENTS AND VARIABILITY

IN VEGETABLE AMARANTH

Umakanta SARKER^1,4, Md. Tofazzal ISLAM², Md. Golam RABBANI³, Shinya OBA⁴

¹Department of Genetics and Plant Breeding, Faculty of Agriculture,Bangabandhu Sheikh Mujibur Rahman Agricultural University,Gazipur-1706, Bangladesh

²Department of Biotechnology,Faculty of Agriculture,Bangabandhu Sheikh Mujibur Rahman Agricultural University,Gazipur-1706, Bangladesh

³Department of Horticulture, Faculty of Agriculture,Bangladesh Agricultural University,Mymensingh-2202, Bangladesh

⁴Laboratory of Field Science, Faculty of Applied Biological Science, Gifu University,Gifu, Japan

Abstract

Sarker Umakanta, Md. Tofazzal Islam, Md. Golam Rabbani, S. Oba (2018): Antioxidant leaf pigments and variability in vegetable amaranth.- Genetika, Vol 50, No.1, 209-220.

Twenty-three vegetable amaranth genotypes were evaluated for variability, interrelationships among antioxidant leaf pigments and foliage yield. Five genotypes found to be a rich source of antioxidant leaf pigments and might be directly selected as antioxidant leaf pigments enriched high yielding varieties. Two genotypes had high content of antioxidant leaf pigments with low yield might be used as donor parents for antioxidant leaf pigments genes to develop transgressive segregant or pigment enriched transgenic vegetable amaranth varieties. The insignificant negative genotypic correlation was observed between total carotene versus all antioxidant leaf pigments, ascorbic acid versus all antioxidant leaf pigments and foliage yield versus rest of all traits. Improvement of vegetable amaranth regarding carotene and ascorbic acid might be possible without compromising yield loss. On the other hand, most of the interrelationships among antioxidant leaf pigments traits indicated that improving of one antioxidant leaf pigment significantly improved the other antioxidant leaf pigments.

Key words: Betalains, carotene, ascorbic acid, chlorophyll a and b, correlation

Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh. E-mail: umakanta@bsmrau.edu.bd

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 221-229

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801221P

Original scientific paper

GENETIC PREDISPOSITIONS OF COMMON ASH TO THE ASH DIEBACK

CAUSED BY ASH DIEBACK FUNGUS

Slavica PAPIĆ¹, Roman LONGAUER^1,², Ivan MILENKOVIĆ^3,4, Jiří ROZSYPÁLEK¹

¹Mendel University, Faculty of Forestry and Wood Technology, Brno, Czech Republic

²National Forest Centre – Forest Research Institute, Zvolen, Slovakia

³Phytophthora Research Centre, Mendel University, Brno, Czech Republic

⁴Institute of Forestry, Belgrade, Serbia

Abstract

Papić S., R. Longauer, I. Milenković, J. Rozsypálek (2018): Genetic predispositions of common ash to the ash dieback caused by ash dieback fungus.- Genetika, Vol 50, No.1, 221-229.

The paper reviews information on ash dieback, a serious disease of common ash and its causing agent ash dieback fungus Hymenoscyphus fraxineus. This paper covers biology and genetics of the causing agent. Main emphasis is given to the genetic predisposition of the tolerance to the disease. Strong genetic control of the infection-tolerance opens the possibility for selection of hyposensitive trees for the establishment of seed orchards, which will produce offspring with improved tolerance to H. fraxineus.

Keywords: disease tolerance, Fraxinus excelsior, Hymenoscyphus fraxineus, resistance

Corresponding author: Slavica Papić, Mendel University, Faculty of Forestry and Wood Technology, Zemědělská 3, 613 00, Brno, Czech Republic, Phone: +420 773640 863, email: papic.papeslavica@yahoo.com; xpapic@node.mendelu.cz

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2017), pp. 231-242

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801231M

Original scientific paper

CHARACTERIZATION AND MOLECULAR DIVERSITY OF IRANIAN RHIZOBIA ISOLATED FROM FABA BEAN

Hamid hatami maleki¹, Kiomars rouhrazi², Gholam khodakaramian²,

Naser sabaghnia¹

¹Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

²Department of Plant protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract

maleki hatami H., K. rouhrazi, G. khodakaramian, N. sabaghnia (2018): Characterization and molecular diversity of Iranian rhizobia isolated from faba bean.- Genetika, Vol 50, No.1, 231-242.

The diversity and phylogeny of 30 rhizobia isolated from nodules of faba bean plants grown on 5 geographic regions located in the East Azerbaijan province of Iran were examined using rep-PCR fingerprinting, sequence analysis of 16S rRNA accompanied with nodC genes. Based on cluster analysis of rep-PCR fingerprints, faba bean rhizobia isolates were differentiated into five clusters (A to E) at 80% similarity level. The cophenetic correlation coefficient for the dendrogram obtained from the combined dataset of BOX and ERIC primers was 0.942. The percentage of polymorphic loci was 59.2% using the BOX-PCR primer and 67.3% using the ERIC-PCR primers. The data obtained by rep-PCR fingerprinting showed high apparent correlation between genetic diversity and geographical origin of the isolates. The phylogenetic analysis based on 16S rRNA and nodC sequences showed that representative isolates were closely related to R. leguminosarum bv. viciae and R. fabae. To the best of our knowledge, this is first report of isolation and characterization of R. fabae from Iran.

Key words: Faba bean, NodC gene, Rep-PCR, 16S rRNA

Corresponding author: Hamid Hatami Maleki, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran. E-mail: hatamimaleki@maragheh.ac.ir, Phone: (+98)9148964510

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 243-251

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801243L

Original scientific paper

VARIABILITY AND HERITABILITY OF MILK TRAITS OF HOLSTEIN - FRISIAN BULL DAMS AND THEIR PROGENY

Marina LAZAREVIĆ^1*, Dragan STANOJEVIĆ², Vladan BOGDANOVIĆ², Vlada PANTELIĆ¹, Nevena MAKSIMOVIĆ¹, Miloš MARINKOVIĆ¹, Nenad MIĆIĆ¹

¹ Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia

² Faculty of Agriculture, University of Belgrade, Belgrade-Zemun, Republic of Serbia

Abstract

Lazarević M., D. Stanojević, V. Bogdanović, V. Pantelić, N. Maksimović, M. Marinković, N. Mićić (2018): Variability and heritability of milk traits of Holstein - frisian bull dams and their progeny.- Genetika, Vol 50, No.1, 243-251.

The research was performed on Holstein-Friesian and Black and White bull dams reared on five farms of Agricultural Corporation of Belgrade - PKB. The study included 575 lactations of cows selected as bull dams and their progeny calved in the period from 2007 - 2014 and represent progeny of 24 bulls. The following dairy traits were analysed in a standard lactation (305 days): milk yield (kg) - MY, milk fat content (%) - % MF, milk fat yield (kg) - MFY, protein content (%) - % PC and protein yield (kg) - PY. Holstein-Friesian bull dams and their progeny, in standard lactation, produced on average 9239.84 ± 1607.64 kg of milk, with a milk fat content of 3.44 ± 0.20 and protein content of 3.21 ± 0.12. The impact of bull - sire, year of birth, lactation order, farm, year and calving season was present at different levels of statistical significance on yield traits, while the genetic group had no influence on any of the milk traits. Bull - sire, year of birth, lactation order and calving season did not influence the variability of milk fat and protein content. Heritability of observed milk traits was medium to low. The content of milk fat and protein had the lowest values of heritability, 0.014, and 0.024, respectively. The heritability of milk yield, milk fat yield and protein yield was 0.293, 0.319 and 0.273, respectively.

Key words: Holstein – Frisian breed, bull dams, milk traits, heritability

Corresponding author: Marina Lazarević, Institute for Animal Husbandry, Autoput 16, P. Box 23, 11080, Belgrade-Zemun, Republic of Serbia, E-mail: marinaplazarevic@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 253-259

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801253R

Original scientific paper

BREEDING GOALS AND SELECTION EFFORT IN THE BREEDING OF LIPIZZAN HORSES IN THE STUD FARM VUČIJAKA FROM 1946 TO 2015

Biljana ROGIĆ*, Božo VAŽIĆ, Đorđe SARAJLIĆ

¹University of Banja Luka, Faculty of Agriculture, Banja Luka, Republic of Srpska, BIH

Abstract

Rogić B., B. Važić, Đ. Sarajlić (2018): Breeding goals and selection effort in the breeding of Lipizzan horses in the stud farm Vučijaka from 1946 to 2015.- Genetika, Vol 50, No.1, 253-259.

The Lipizzan horse belongs to the oldest European horse breeds. In the year 1946 the Bosnian Lipizzan state stud farm Vučijak, located next to the town Prnjavor, was founded. The foundation stock originated from the Croatian state stud farms Lipik and Đakovo, and from private owners in Croatia. The breeding goal of Vučijak was to obtain a Lipizzan horse of a smaller body frame suitable for driving and the use as pack horses. The aim of this study was to compare anatomical body measurements of the founder animals with measurements of actual breeding population. From in total 41 horses (10 stallions and 31 mares) following body measurements were taken: height at withers (measured by tape), circumference of chest and circumference of cannon bone forelimb. All horses was 4 years or older at the time of measuring. In order to compare the actual breeding population with the foundation population we extracted comparable data of 17 stallions and 36 mares from the stud book. On the basis of the morphological measures, compactness and bonines index were calculated. The results showed that today's Lipizzan horse of the Vučijak stud are smaller in height at whiters and circumference of chest, while the circumference of cannon bone is larger than documented for founders. The t-test showed a statistically significant difference in the height at whiters and circumference of cannon bone. On the basis of the obtained results, it can be concluded that today's Lipizzan from the Bosnian stud farm Vučijak have a smaller body frame, which confirmed selection success.

Keywords: Lipizzan horse, morphological body measurements, stud farm Vučijak

Corresponding author: Biljana Rogić, University of Banja Luka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1a, Banja Luka, Republic of Srpska, Bosnia nad Herzegovina

biljana.rogic@agro.unibl.org, Phone: 0038765286564

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 261-274

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1801261K

Original scientific paper

Ecologo-genetical model for control of the quantitative traits in white lupin

Valentin kosev and Viliana vasileva

Institute of Forage Crops, Pleven, Bulgaria

Abstract

kosev V. and V. vasileva (2018): Ecologo-genetical model for control of the quantitative traits in white lupin.- Genetika, Vol 50, No.1, 261-274.

An attempt a transition from a selection based on the concepts of "gene-character" to selection of genetic-physiological systems was done suggesting an ecologo-genetical model for control of the quantitative traits in white lupin. Plant material of the aboveground and root mass of 7 varieties of white lupin different originated was analyzed: PI457923 (Greece), PI368911 (Czech Republic), PI533704 (Spain), PI457938 (Morocco), KALI (Poland), Zuter (France) and Lucky801 (France). The study was performed on the experimental field of the Institute of Forage Crops, Pleven, Bulgaria during three subsequent years (2014-2016). The ecologo-genetical models for organization of the quantitative traits and method of the orthogonal regressions were applied as methods for assessment. The highest average seed yield was found at Lucky801 (208.67 kg/da) and PI533704 (151.92 kg/da). PI368911, PI457938 and KALI were characterized by high variability and seed yields (90.00 kg/da, 80.08 kg/da, 83.25 kg/da) below the average ones for the tested group of varieties. PI533704 and PI457938 varieties showed the highest fresh aboveground mass yields (485.12 and 597.98 kg/da). Plants of PI457938 formed high fresh aboveground and root mass weight and exhibit a good combination of adaptability and attraction genes. Lucky801 and PI533704 varieties showed strong genes of the physiological systems attractiveness and adaptability by seed weight and root mass weight per plant, but PI457923 and Zuter were well adapted. These varieties were of greatest interest and may be included in future hybridization schemes to obtain hybrids combining in one genotype high seed and root mass weight.

Keywords: ecologo-genetical models, genotype, productivity, regression, white lupin

Corresponding author: Valentin Kosev, Institute of Forage Crops, 89 “General Vladimir Vazov” Str., 5800 Pleven, Bulgaria, E-mail and phone are: valkosev@hotmail.com and + 359 886021598

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp 275-284

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801275B

Original scientific paper

GENETIC EVALUATION OF BODY WEIGHT TRAITS IN IRANIAN NATIVE GHEZEL SHEEP

H. baneh¹* and J. ahmadpanah²

¹Department of Animal breeding and genetic, Animal Science Research Institute of IRAN (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

²Young Researchers and Elite Club, Ilam Branch, Islamic Azad University, Ilam, Iran.

Abstract

baneh H. and J. ahmadpanah (2018): Genetic evaluation of body weight traits in Iranian native Ghezel sheep.- Genetika, Vol 50, No.1, 275-284.

The aim of the current study was to estimate genetic parameters and trends for body weight traits at different ages in Ghezel lambs. Traits included were birth weight (BW), 3-month weight (3MW), 6-month weight (6MW), 9-month weight (9MW), and yearling weight (YW). Data and pedigree information used in the present study were collected at the Breeding Station of Ghezel sheep during 1986–2009. (Co) variance components were estimated using kosev V. and V. vasileva (2018): Ecologo-genetical model for control of the quantitative traits in white lupin REML procedure and breeding values of animals were predicted with Best Linear Unbiased Prediction (BLUP) methodology under univariate analysis. Three different animal models were fitted. The models consisted of the direct additive genetic effect but differed in combinations of maternal additive genetic and maternal permanent environmental effects. Genetic trends for each trait were obtained by regressing the means of predicted breeding values on year of birth. The estimates of direct heritability were 0.285, 0.371, 0.388, 0.450 and 0.179, respectively. Also, the maternal heritability was estimated 0.113, 0.031, 0.021 and 0.030 for BW, WW, 6MW and 9MW, respectively. Direct genetic trends were positive and significant for BW, WW, 6MW (p<0.01), 9MW and YW (p<0.05) and were obtained 2.34, 46.20, 55.11, 33.40 and 24.01 (g/year), respectively. Also, maternal trends for BW, WW, 6MW and YW were positive and highly significant (p<0.01) and were 3.37, 17.05, 12.56 and 16.30 (g/year), respectively. The results indicated that considering maternal effects in the statistical model make accurate estimates of genetic parameters. Also, improvement of growth traits in Ghezel sheep seems to be likely in selection programs.

Key words: Best Linear Unbiased Prediction, Ghezel sheep, genetic parameters, genetic trends, growth traits

Corresponding author: H. baneh, Department of Animal breeding and genetic, Animal Science Research Institute of IRAN (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran, email: hasanbaneh@gmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 285-298

UDC 575.633.11

https://doi.org/10.2298/GENSR1801285J

Original scientific paper

EFFECT OF SALINITY AND DROUGHT STRESS ON GERMINATION AND EARLY SEEDLINGS GROWTH OF BREAD WHEAT (Triticum aestivum L.)

Mirjana jovović¹, Vesna tunguz¹, Milan mirosavljević²,Novo pržulj^3,1

¹University in East Sarajevo, Faculty of Agriculture, Bosnia and Herzegovina

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³University in Banja Luka, Faculty of Agriculture, Bosnia and Herzegovina

Abstract

jovović M., V. tunguz, M. mirosavljević,N. pržulj (2018): Effect of salinity and drought stress on germination and early seedlings growth of bread wheat (Triticum aestivum L.).- Genetika, Vol 50, No.1, 285-298.

Salinity is one of the most important environmental stresses that limits soil fertility and has adverse effects on plant growth. In order to increase wheat production under saline conditions, it is necessary to better understand genotypic difference, the physiological and biochemical processes of salt tolerance in wheat. The aim of this study was to determine the most tolerant winter wheat varieties against salinity and osmotic stress at germination stage and early seedlings growth. The salinity and osmotic stress were simulated in controlled environmental conditions by adding different concentrations of NaCl and mannitol solution to the growing media of five winter wheat variety. In all studied varieties the benchmark water potential in which they had germinated and had a good seedlings growth was of -0.3MPa. Under the stronger stress, -0.6 MPa, all varieties showed reduction in the all examined parameters. The variety Bosanka, had the highest final germination and germination energy under both mannitol and salt stress treatments. The parameters defining the development or percentage of strong seeds, coleoptile and root length, fresh and dry weight of root and coleoptile of a seed were more affected by water deficit stress and salt stress than germination and germination energy. Biplot analysis showed that wheat cultivars grown under -0.6MPa osmotic had higher values of root/coleoptile ratio in relation to control and -0.3MPa treatment which is the most reliable for screening properties of the genotypes for drought resistance in seedling stage.

Key words: Biplot analysis, genotype, germination, mannitol, NaCl, tolerance, Triticum aestivum

Corresponding author: Mirjana Jovović, University in East Sarajevo, Faculty of Agriculture, e-mail:mirjanamojevic@gmail.com, +38765094774

Return to content

Published in „GENETIKA“ Vol. 50, No. 1 (2018), pp. 299-315

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1801299C

Original scientific paper

INHERITANCE OF INORGANIC AND PHYTIC PHOSPHORUS

IN MAIZE (Zea mays L.) KERNEL

Zoran CAMDZIJA*, Vesna DRAGICEVIC, Jelena VANCETOVIC, Milan STEVANOVIC, Jovan PAVLOV, Milomir FILIPOVIC and Dragana IGNJATOVIC-MICIC

Maize Research Institute “Zemun Polje”, Zemun Polje – Belgrade, Serbia

Abstract

Camdzija Z., V. Dragicevic, J. Vancetovic, M.Stevanovic, J. Pavlov, M. Filipovic and D. Ignjatovic-Micic (2018): Inheritance of inorganic and phytic phosphorus in maize (Zea mays L.) kernel.- Genetika, Vol 50, No.1, 299-315.

A set of fifteen elite inbred lines of maize (used as mothers) and three tester inbred lines (used as fathers) were investigated using line x tester statistical model, including both hybrids and inbreds. Four traits were measured: grain yield, 1000 kernel weight, phytic phosphorus (P_phy) and inorganic phosphorus (P_i) in the kernel. P_phy content among hybrids ranged from 2.342 to 4.812 g kg^-1 and P_i content from 0.562 to 2.340 g kg^-1, while among inbreds (lines and testers) they ranged from 2.503 to 4.180 g kg^-1 and from 0.587 to 1.629 g kg^-1, respectively. Correlations between the four traits allow breeding for high P_i and low P_phy, as well as for both high P_i and phytate, without compromising grain yield. Correlation for hybrids between P_phy and P_i was 0.185 (p<0.05) and for inbreds 0.142 (non-significant). General combining ability / special combining ability (GCA/SCA) values for all the traits were below 1 (very low) indicated non-additive inheritance. In the investigated set of genotypes, multiple selection indices should be used for simultaneous improvement of grain yield and phosphorus profile of maize grain.

Key words: line x tester analysis, phytate, inorganic phosphorus, phytic phosphorus

Corresponding author: Zoran Camdzija, Maize Research Institute “Zemun Polje”, Slobodana Bajica 1, 11185 Zemun Polje – Belgrade, Serbia, e-mail: zcamdzija@mrizp.rs, cell phone: +381 64 840 6032

Return to content

Published in „GENETIKA“ Vol. 50, No. 1(2018), pp. 317-323

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801317A

Original scientific paper

POLYMORPHISM OF THE MELANOCORTIN RECEPTOR GENE AND ITS ASSOCIATION WITH EGG PRODUCTION TRAITS IN LOHMANN BROWN CHICKENS

Sarah AGGAG¹ and Karim EL-SABROUT²

¹Department of Genetics, Faculty of Agriculture (El-Shatby), University of Alexandria, Alexandria, Egypt

²Department of Poultry Production, Faculty of Agriculture (El-Shatby), University

of Alexandria, Alexandria, Egypt

Abstract

Aggag S. and K. El-Sabrout (2018): Polymorphism of the melanocortin receptor gene and its association with egg production traits in Lohmann brown chickens.- Genetika, Vol 50, No.1, 317-323.

The melanocortin gene (MC4R) was proposed as a candidate gene in this study for egg production traits (yield and weight) in Lohmann Brown hens. Two different primers from MC4R gene (MC4R-1 and MC4R-2) were investigated. DNA from blood samples was extracted to amplify the MC4R gene and the purified PCR products were sequenced. Alignment of sequence data from each group revealed that there is a variation in MC4R-1 at nucleotide 22 (T-G) (sense mutation) for high egg weight. Hens with the AB genotype produced significantly (P<0.05) higher egg weight compared to hens with the AA and BB genotypes. There was no significant (P>0.05) effect of this mutation on egg yield. There was no variation detected in MC4R-2. The detected mutation and the analysis of egg production means revealed a significant association between MC4R polymorphism and egg weight. The MC4R-SNP could be considered as a useful marker in chicken selection especially for egg weight.

Keywords: egg weight, Lohmann chickens, MC4R, sense mutation, SNP

Corresponding author: Karim El-Sabrout, Department of Poultry Production, Faculty of Agriculture (El-Shatby), University of Alexandria, Alexandria, Egypt, Tel: +20108984822, E-mail: kareem.badr@alexu.edu.eg

Return to content

Published in „GENETIKA“ Vol. 50 No. 1 (2018), pp. 325-332

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801325S

Original scientific paper

INVESTIGATION OF ASSOCIATION BETWEEN THE HUMAN NEUROPEPTIDE Y GENE LEUCINE7PROLINE POLYMORPHIM AND OBESITY IN A POPULATION FROM TURKEY

Mehmet Ali SÖZEN¹, Yasemin ÇETİNKAYA², Gizem GEÇGEL², Nuriye ÖZCAN²,

Ersin ÜLGER²

¹Department of Medical Biology, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey

²School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey

Abstract

Sözen M. A., Y. Çetinkaya, G. Geçgel, N. Özcan, Ersin Ülger (2018): Investigation of association between the human neuropeptide y gene leucine7proline polymorphism and obesity in a population from Turkey.- Genetika, Vol 50, No.1, 325-332.

Obesity, an important public health issue, is a risk factor for many diseases and has been associated with many genes. The aim of this study was to investigate if there is an association between an NPY gene polymorphism, Leu7Pro, and obesity and/or obesity-related phenotypes. A total of 84 obese cases (45 female and 39 male) and a total of 77 non-obese control subjects (38 female and 39 male) were included in this case-control study. Body weight and height measurements were used for calculation of Body Mass Index (BMI) and the ones in the ranges of 18 to 25 kg/m² were considered normal and the ones 30 kg/m² and over were considered obese from the subjects.

Minor allele frequency for Leu7Pro polymorphism was 3.5% and it was found to be associated to increased obesity in the population studied. No significant differences were observed between genotype distrubutions and allele frequencies for both obese and non-obese subjects. However, mean BMI values were found to be higher (38,88 ± 2,96) in obese cases having Pro7 allele than non carriers of this allele (35,37 ± 5,16), (p=0.044). This is the first study in a Turkey population which supports the role of Leu7Pro polymorphism in obesity. Further studies with larger sample sizes may confirm these findings and can contribute and shed light on the genetic factors playing a part in Turkey population.

Key words: association analysis, neuropeptide Y gene, NPY polymorphism, obesity

Corresponding author: Mehmet Ali Sözen, Ph.D., Department of Medical Biology, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey, Tel: (272) 246 3301 Fax: (272) 246 3300, E-mail address: masozen@hotmail.com

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp. 333-350

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1801333W

Review paper

From proteomics to ionomics: Soybean genetic improvement

for better food safety

Daisuke watanabe¹, Tomáš lošáK², Johann vollmann¹

¹ Plant Breeding Division, Department of Crop Sciences, University of Natural Resources

and Life Sciences Vienna (BOKU), Tulln an der Donau, Austria

² Department of Environmentalistics and Natural Resources, Faculty of Regional Development and International Studies, Mendel University in Brno, Brno, Czech Republic

Abstract

watanabe D., T. lošák, J. vollmann (2018): From proteomics to ionomics: soybean genetic improvement for better food safety.- Genetika, Vol 50, No.1, 333-350.

Soybean is a major protein and oilseed crop for food and livestock feed production, which is increasingly utilized in the food industry due to its favorable protein content and a superior overall seed composition with a high nutritional value. However, some of the soybean seed components have the potential to reduce the value of soy-food products as they are posing different food safety risks. Therefore, the objective of the present review was to evaluate options of soybean genetic improvement for the development of food-grade soybeans with a focus on food safety traits. To date, useful genetic variation in soybean germplasm collections and breeding materials has been described for protein components such as allergens or anti-nutritional factors, for fatty acid composition relevant to food safety, and for toxic heavy metal accumulation. Due to the progress in genomic research, genetic markers are available for assisting the introgression of major food safety traits into breeding populations, and the genetic mechanisms behind particular food safety traits have been clarified. Moreover, analytical methods from the fields of proteomics or ionomics are helpful for validating selection response and for monitoring quality features across genotypes. As consumer demand for food safety is steadily increasing, plant breeding approaches are gaining in importance as they can provide high-quality soybean raw materials to the food industry. For implementing better food safety on the consumer level, however, it appears that coordinated action between plant breeding and genetic research, food processing and marketing of products needs to be developed.

Key words: allergen elimination, cadmium concentration, food safety, Glycine max, seed protein composition, soybean.

Corresponding author: Johann Vollmann, Plant Breeding Division, Department of Crop Sciences, University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenz Str. 24, 3430 Tulln an der Donau, Austria, Phone +43 1 47654 95717, E-mail: johann.vollmann@boku.ac.at

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp.351-356

Serbia

UDC 575

https://doi.org/10.2298/GENSR1801351H

Short communication

TESTING OF DIFFERENT IRON SOURCES AND CONCENTRATIONS on shoot MULTIPLICATION OF blackberry (Rubus fruticosus L.)

Júlia HUNKOVÁ^1,2, Gabriela LIBIAKOVÁ¹, Jozef FEJÉR³, Tatjana VUJOVIĆ⁴,

Alena GAJDOŠOVÁ¹

¹Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Nitra, Slovak Republic

²Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic

³Faculty of Humanities and Natural Sciences, University of Prešov, Prešov, Slovak Republic

⁴Fruit Research Institute, Čačak, Serbia

Abstract

Hunková J., G. Libiaková, J. Fejér, T. Vujović, A. Gajdošová (2018): Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.). - Genetika, Vol 50, No.1, 351-356.

The aim of this work was to evaluate shoot multiplication of two blackberry (Rubus fruticosus L.) cultivars ‘Black Satin’ and ‘Loch Ness’ on two culture media: Murashige & Skoog (MS) and its modification Murashige & Skoog Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference in shoot multiplication between different iron sources for both tested cultivars. For ‘Black Satin’ it was shown that double concentration of chelates FeNaEDTA and FeEDDHA in culture media negatively affected on shoot growth and multiplication. These results can be very useful for further optimization of micropropagation process for different Rubus cultivars.

Key words: Rubus, cultivar, shoot proliferation, iron, chelate

Corresponding author: Alena Gajdošová, Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Nitra, Slovak Republic, E-mail and phone: alena.gajdosova@savba.sk, +421 37 9643 315

Return to content

Published in „GENETIKA“ Vol. 50, No.1 (2018), pp.357

Serbia

Genetika, 50(1), pp. 357, 2018

https://doi.org/10.2298/GENSR1801357V

CORRIGENDUM

https://doi.org/10.2298/GENSR1703801V

The authors of the above-referenced article have requested a corrigendum to it. The article should include the following correction in email of corresponding authors: insteed svetlana.vojvodic021@gmail.com correct email is svetlana.vojvodic@mf.uns.ac.rs.

UDC 575

https://doi.org/10.2298/GENSR1703801V

Original scientific paper

IMPACT OF HLA -B*27 SUBTYPES WITH INCREASED SUSCEPTIBILITY TO SERONEGATIVE SPONDYLOARTHROPATHIES IN VOJVODINA POPULATION

Svetlana vojvodić¹, Dušica ademović-sazdanić¹

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, University of Novi Sad, Medical faculty Novi Sad

Surma M., T. Adamski, Z. Kaczmarek, W. Święcicki, S. Mejza, P. Barzyk, A. Kuczyńska, K. Krystkowiak, K. Mikołajczak, P. Ogrodowicz (2017): A multivariate approach to the selection of pea (Pisum sativum L.) lines obtained by the single seed descent technique.- Genetika, Vol 49, No.1., 365-376.

Corresponding author: Svetlana Vojvodić, M.D., Ph.D., Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Compartment, Hajduk Veljkova 9a, 21000 Novi Sad, Serbia; Tel.: +381-21-4877-963; Fax: +381-21-4877-978; E-mail: svetlana.vojvodic@mf.uns.ac.rs.

Return to content