GENETIKA, Vol

GENETIKA, Vol. 50, No. 2(2018)

Nikola GRČIĆ, Nenad DELIĆ, Milan STEVANOVIĆ, Jovan PAVLOV, Miloš CREVAR, Marko MLADENOVIĆ, Nemanja NIŠAVIĆ
GENETIC DISTANCE OF MAIZE INBRED LINES BASED ON SSR MARKERS FOR PREDICTION OF HETEROSIS AND COMBINING ABILITY [Abstract] [Full text]

Tahira BIBI, Hafiz Saad Bin MUSTAFA, Tariq MAHMOOD, Amir HAMEED and Qurban ALI
MULTIVARIATE ANALYSIS FOR ADAPTABILITY AND YIELD STABILITY OF RAPESEED (Brassica napus L.) STRAINS IN DIFFERENT AGRO-CLIMATIC ZONES [Abstract] [Full text]

Sanja MIKIĆ, Ankica KONDIĆ-ŠPIKA, Ljiljana BRBAKLIĆ, Dušan STANISAVLJEVIĆ, Dragana TRKULJA, Marina ĆERAN, Bojan MITROVIĆ
ASSOCIATION ANALYSIS OF AGRONOMIC TRAITS WITH MICROSATELLITES IN MAIZE INBRED LINES [Abstract] [Full text]

Hüseyin UYSAL, Zeki ACAR, İlknur AYAN, Orhan KURT
GENETIC DIVERSITY OF TURKISH Lathyrus L. LANDRACES USING ISSR MARKERS [Abstract] [Full text]

Rosa EFTEKHARIAN, Masoud SHEIDAI, Farideh ATTAR, Zahra NOORMOHAMMADI, Somayyeh GHASEMZADEH-BARAKI

MOLECULAR AND MORPHOLOGICAL STUDY OF THE GENUS Senecio L. (ASTERACEAE: SENECIONEAE) IN IRAN [Abstract] [Full text]

Dragana MILADINOVIĆ, Marko MILER, Ana MARJANOVIĆ JEROMELA, Ivana IMEROVSKI, Aleksandra DIMITRIJEVIĆ, Branislav KOVAČEVIĆ, Siniša JOCIĆ, Sandra CVEJIĆ, Nada HLADNI, Dragana OBREHT-VIDAKOVIĆ

EVALUATION OF RAPD MARKERS AS A MARKER-ASSISTED SELECTION TOOL FOR VARIETY TYPE AND ERUCIC ACID CONTENT IN RAPESEED [Abstract] [Full text]

Ali MOHAMMADI, Yousef NADERI, Reza NABAVI and Fatemeh JAFARI

DETERMINATION OF THE BEST MODEL FOR ESTIMATION OF GENETIC PARAMETERS ON THE FARS NATIVE CHICKEN TRAITS USING BAYESIAN AND REML METHODS[Abstract] [Full text]

Fatemeh BAVANDPORI, Jafar AHMADI, Sayyed Mohssen HOSSAINI

STABILITY ANALYSIS OF BREAD WHEAT LANDRACES AND LINES USING BIOMETRICAL GENETIC MODELS [Abstract] [Full text]

Mirjana BOJANIC RASOVIC, Sigrid MAYRHOFER, Mary A.A. OCHOME, Erna AJANOVIC, Marija ZUNABOVIC, Aleksandra MARTINOVIC, Konrad J. DOMIG

DIVERSITY OF LACTIC ACID BACTERIA ISOLATED FROM TRADITIONAL MONTENEGRIN DAIRY PRODUCTS [Abstract] [Full text]

Wajeeha AMIN, Sajjad HUSSAIN, Muhammad Akbar ANJUM, Shaghef EJAZ, Muhammad SAQIB, Muhammad Fasih KHALID, Sezai ERCISLI, Shakeel AHMAD
GENETIC DIVERSITY OF JUJUBE (Ziziphus mauritiana) CULTIVARS [Abstract] [Full text]

Emina mladenović Jelena čukanović, Biljana božanić-tanjga, Lazar pavlović, Ksenija hiel, Mirjana mirić

SELECTION OF GARDEN ROSE CULTIVARS FOR USE AS A CUT FLOWER [Abstract] [Full text]

Wanqing WANG, Ke WANG, Xi CHEN, Slaven PRODANOVIC, Xiaohui LI, Xingguo YE, Yueming YAN
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF ONE OMEGA-GLIADIN GENE FROM Aegilops speltoides L. [Abstract] [Full text]

Vladislava GALOVIĆ, Miroslav MARKOVIĆ, Predrag PAP, Martin MULETT, Milana RAKIĆ, Aleksandar VASILJEVIĆ, Saša PEKEČ

MOLECULAR TAXONOMY AND PHYLOGENETICS OF Daedaleopsis confragosa (Bolt.: Fr.) J. Schröt. FROM WILD CHERRY IN SERBIA [Abstract] [Full text]

Fatemeh AZIMISHAD, Masoud SHEIDAI, Seyed Mehdi TALEBI, Zahra NOORMOHAMMADI

BIOSYSTEMATIC STUDY AND POPULATION GENETIC ANALYSIS IN Lamium amplexicaule L. (LAMIOIDEAE, LAMIACEAE) [Abstract] [Full text]

Dragan NIKOLIĆ, Tijana BANJANIN, Zorica RANKOVIĆ-VASIĆ

VARIABILITY AND HEREDITY OF SOME QUALITATIVE AND QUANTITATIVE GRAPEVINE CHARACTERISTICS [Abstract] [Full text]

Onur HOCAOĞLU, Yalçın COŞKUN

Evaluatıon OF dry matter accumulatıon IN trıtıcale by dıfferent sıgmoıdal growth models ın West Anatolıa of Turkey[Abstract] [Full text]

Sevda BABAYEVA, Zeynal AKPAROV, Litfer AMIROV, Kamila SHIKHALIYEVA, Saida HASANOVA, Khanbala RUSTAMOV, Rufat MIRZAYEV, Vusala IZZATULLAYEVA, Ilhama MIRZALIYEVA, Afig MAMMADOV, Mehraj ABBASOV
GENETIC RELATIONSHIP AMONG INTRODUCED LENTIL GERMPLASM USING AGRONOMIC TRAITS AND ISSR MARKERS [Abstract] [Full text]

Velimir MLADENOV, Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Jan BOĆANSKI, Ankica KONDIĆ-ŠPIKA, Dragana TRKULJA, Borislav BANJAC

Agronomic performance of wheat cultivars and their molecular characterization [Abstract] [Full text]

Emre SEVİNDİK

GENOME-WIDE IDENTIFICATION AND ANALYSIS OF RUBISCO LARGE SUBUNIT PROTEINS IN Morus L. (MORACEAE) SPECIES [Abstract] [Full text]

Mehrangiz FATHI, Ali HASHEMI, Ghorban ELYASEI ZARRIN GHOBAYI

ASSOCIATION ANALYSIS BETWEEN GENETIC DIVERSITY OF THE MELANOCORTIN4 RECEPTOR (MC4R) GENE AND PRODUCTION TRAITS IN TURKEY [Abstract] [Full text]

Farah FARAHANI, Masoud SHEIDAI, Fahimeh KOOHDAR

GENETIC FINGER PRINTING OF COTTON CULTIVARS BY ISSR MOLECULAR MARKERS[Abstract] [Full text]

Vladan ugrenović, Marija bodroža solarov, Lato pezo, Jovana đisalov, Vera popović, Boško marić, Vladimir filipović

ANALYSIS OF SPELT VARIABILITY (Triticum spelta L.) GROWN IN DIFFERENT CONDITIONS OF SERBIA BY ORGANIC CONDITIONS [Abstract] [Full text]

Iman SHAHROKHIYAN, Abbas DOOSTI, Hossein SAZEGAR

EVALUATION OF THE EFFECT OF AMYGDALIN ON survivin GENE EXPRESSION IN MCF-7 BREAST CANCER CELL LINE [Abstract] [Full text]

Dragan NIKŠIĆ, Vlada pantelić, Dušica ostojić-andrić,Predrag perišić,
POLYMORPHISM OF K-CASEIN AND B-LACTOGLOBULINE IN SIMMENTAL CATTLE IN SERBIA [Abstract] [Full text]

Sezgin AYAN, Erkan ÜNALAN, Oytun Emre SAKICI, Esra Nurten YER, Fulvio DUCCI, Vasilije V. ISAJEV, Halil Barıs OZEL

PRELIMINARY RESULTS OF TURKISH HAZELNUT (Corylus colurna L.) POPULATIONS FOR TESTING THE NUT CHARACTERISTICS [Abstract] [Full text]

Tijana Banjanac, Marijana Skorić, Mario Belamarić, Jasmina Nestorović Živković, Danijela Mišić, Mihailo Jelić, Slavica Dmitrović, Branislav Šiler

PURSUIT FOR EST MICROSATELLITES IN A TETRAPLOID MODEL FROM DE NOVO TRANSCRIPTOME SEQUENCING [Abstract] [Full text] [Supplementary Table]

Slavica POPOVIĆ BUBUJUK, Ninoslav DJELIĆ, Adrian MUSTER, Dragan KATARANOVSKI, Marko ANDJELKOVIĆ
INFLUENCE OF CADMIUM CHLORIDE ON THE FREQUENCY OF MICRONUCLEI IN DA AND AO RATS [Abstract] [Full text]

Alinaghi MIRMOAYEDI, Fatemeh RASHIDIKHAH, Danial KAHRIZI, Kheirullah YARI

GENETIC RELATIONSHIP BETWEEN NEUROPTERAN FAMILIES (INSECTA, NEUROPTERIDA, NEUROPTERA) BASED ON CYTOCHROME OXIDASE-I SEQUENCES [Abstract] [Full text]

Dragan marinković, Tatjana marinković
c-Myc MISREGULATION TRIGGERS COMPLEX PROCESS OF GENOMIC INSTABILITY[Abstract] [Full text]

Ivanka HABUŠ JERČIĆ, Marijana BARIĆ, Snježana KEREŠA, Anita BOŠNJAK MIHOVILOVIĆ, Milan POLJAK, Boris LAZAREVIĆ

EFFECT OF TERMINAL DROUGHT ON YIELD AND SOME PHYSIOLOGICAL TRAITS OF WINTER WHEAT [Abstract] [Full text]

dragan mARINKOVIĆ

SERBIAN GENETIC SOCIETY (1968 – 2018) [Full text]

Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 359-368

UDC 575.633.15
https://doi.org/10.2298/GENSR1802359G

Orginal scientific paper

GENETIC DISTANCE OF MAIZE INBRED LINES BASED ON SSR MARKERS FOR PREDICTION OF HETEROSIS AND COMBINING ABILITY

Nikola GRČIĆ*, Nenad DELIĆ, Milan STEVANOVIĆ, Jovan PAVLOV, Miloš CREVAR, Marko MLADENOVIĆ, Nemanja NIŠAVIĆ

Maize Research Institute Zemun Polje, Belgrade, Serbia

Abstract

Grčić N., N. Delić, M. Stevanović, J. Pavlov, M. Crevar, M. Mladenović, N. Nišavić (2018): Genetic distance of maize inbred lines based on SSR markers for prediction of heterosis and combining ability.- Genetika, Vol 50, No.2, 359-368.

Genetic distance among six elite maize inbred lines was analyzed using SSR markers. Hybrid progeny obtained by crossing inbred lines according an incomplete diallel design was tested in field trials together with inbred lines per se.The objective of this study was to determine genetic distance of inbred maize lines and to examine if a significant correlation exist between the genetic distance of parental lines and the exhibited high parent heterosis (HPH) and specific combining abilities (SCA) for grain yield, ear lenght, kernel row numberand number of kernels per row. Twenty one SSR primers were used for genetic assesment of inbreds with detected 92 alleles. Genetically most distant lines were ZPL1 and ZPL5 and ZPL6 with the GD value of 0.549, while the closest one were ZPL2 and ZPL3 with GD value of 0.11. The dendrogram distinguished two main groups of inbreds: ZPL5 and ZPL6 grouped in a smaller cluster and ZPL1, ZPL2, ZPL3 and ZPL4 forming the second cluster. Values of the Spearman’s rank correlation coefficient between genetic distance among inbred lines based on SSR markers and SCA for all analyzed traits were positive and significant with the exception of rows per ear. Highest correlation was exhibited between the genetic distance and SCA for number of kernels per row (0.643). Spearman’s rank correlation coefficient between GD and high parent heterosis was positive and significant for ear length and kernel number in row with coefficient values of 0.554 and 0.611, respectively.

Keywords: inbred lines, maize, SSR markers, genetic distance, heterosis

Corresponding author: Nikola Grčić, Maize Research Institute “Zemun Polje”, Slobodana Bajica 1, 11185 Zemun Polje – Belgrade, Serbia, e-mail: ngrcic@mrizp.rs, cell phone: +381 64 840 6036

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp.369-378

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802369B
Original scientific paper

MULTIVARIATE ANALYSIS FOR ADAPTABILITY AND YIELD STABILITY OF RAPESEED (Brassica napus L.) STRAINS IN DIFFERENT AGRO-CLIMATIC ZONES

Tahira BIBI¹, Hafiz Saad Bin MUSTAFA^1*, Tariq MAHMOOD¹, Amir HAMEED²

and Qurban ALI^3,4

¹Directorate of Oilseeds, Ayub Agricultural Research Institute, Faisalabad, Pakistan

²Statistical Section, Ayub Agricultural Research Institute, Faisalabad, Pakistan

³Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan

⁴Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan

Abstract

Bibi T., H. S. Bin Mustafa, T. Mahmood, A. Hameed and Q. Ali (2018): Multivariate analysis for adaptability and yield stability of rapeseed (Brassica napus L.) strains in different agro-climatic zones.- Genetika, Vol. 50, No. 2, 369-378.

Plant breeders always look for high yielding genotypes based on both genotype and G × E (interaction of genotype with environment) for stability and adaptability in diversified environmental conditions. This present study consists of eight Brassica napus L. strains using RCBD design with three replications and conducted at eight locations in Punjab province, Pakistan to determine general and specific adaptability in 2014-2015. Additive main effects and multiplicative interactions (AMMI) study showed that the environments (86.65%) have more influence to treatment sum of squares as compare to the interaction of genotype with environment (9.4%) and genotypes (2.65%) respectively, indicating the presence of adequate genetic variation for useful selection to identify stable genotypes. G x E interaction was further partitioned by principal component analysis (PCA). The first four multiplicative axis terms (IPCA1, IPCA2, IPCA3 and IPCA4) give detailed 54.0%, 28.0%, 9.9% and 5.6% of GEI sum of squares (SS), respectively. The AMMI method was used to recognize suitable genotype / genotypes to specific locations / environments. The results showed that genotypes RBN-08004, RBN-04021 and 11-CBN 006 were more stable with the lowest interaction and have general adaptability with yield near about their mean yield. Genotype RBN-08004 has more than average yield, IPCA value closer to zero (1.8), genotype selection index (GSI) is 4 and least AMMI stability value (ASV) of 3.6 therefore considered the most stable. According to AMMI analysis, KN-253, KN-256 and RBN-08004 (adaptive group 1) exhibited specific adaptability for Bhakar and Khanpur with yield greater than mean and positive interaction. Genotype RBN-04047 (adaptive group 2) which has yield lesser than mean yield and positive interaction showed specific adaptability for Chakwal and Karore. It was observed that stability evaluation through AMMI analysis may be used for selection of genotypes from experiments performed under different environmental conditions.

Keywords: AMMI, brassica, GE interaction, stability analysis, seed yield

Corresponding author: Hafiz Saad Bin Mustafa,¹Directorate of Oilseeds, Ayub Agricultural Research Institute, Faisalabad, Pakistan, Email: saadpbg@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp.379-394

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1802379M

Original scientific paper

ASSOCIATION ANALYSIS OF AGRONOMIC TRAITS WITH MICROSATELLITES IN MAIZE INBRED LINES

Sanja MIKIĆ, Ankica KONDIĆ-ŠPIKA, Ljiljana BRBAKLIĆ, Dušan STANISAVLJEVIĆ, Dragana TRKULJA, Marina ĆERAN, Bojan MITROVIĆ

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Mikić S., A. Kondić-Špika, Lj. Brbaklić, D. Stanisavljević, D. Trkulja, M. Ćeran, B. Mitrović (2018): Association analysis of agronomic traits with microsatellites in maize inbred lines.- Genetika, Vol 50, No.2, 379-394.

Association analysis or linkage disequilibrium mapping is a method for identification of quantitative trait loci (QTLs) in a panel of divergent unrelated individuals based on historical recombinations during a crop’s domestication and selection. It should account for the population structure, which can be the result of adaptation to local conditions or selection, to reduce the possibility of declaring false-positive associations. The aim of this study was to determine potentially significant and consistent associations between markers and agronomic important maize (Zea mays L.) traits using association analysis in a diverse breeding material that can be ultimately implemented in maize selection. To this end, 96 maize inbred lines were evaluated in field trials at three locations in Serbia for eleven agronomic traits and analysed with microsatellite markers. Twenty five microsatellites were used to assess the population structure using Bayesian model-based clustering method and to test the significance of associations between the markers and the traits with general (GLM) and mixed linear (MLM) models. The cluster analysis divided maize inbred lines in four subpopulations, corresponding to the BSSS (Iowa Stiff Stalk Synthetic), LSC (Lancaster Sure Crop), Iodent heterotic groups and exotic and independent germplasm. The models identified associations between twenty five microsatellite markers and eleven agronomic traits, resulting in 133 and 71 associations across the environments for GLM and MLM, respectively. Some of the identified marker-trait associations were significant and consistent in several environments. The associations stable in several environments were identified between the markers bnlg1067 and two flowering traits; nc005 and bnlg434 and plant height, bnlg434 and ear height; bnlg1643 and umc1127 and leaf number, bnlg1360 and ear diameter; umc1019 and umc1506 and number of rows per ear; bnlg2305 and bnlg1451 and ear length, and between bnlg1175 and thousand-kernel weight. The results of this study indicate that these microsatellites could be used in marker-assisted selection of inbred lines, after validation of the marker-trait associations and testing combining abilities of the inbreds during hybrid development.

Key words: association mapping, microsatellites, yield components, Zea mays L.

Corresponding author: Sanja Mikić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 021 4898 233, e-mail: sanja.mikic@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 50, No2 (2018), pp. 395-402

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802395U

Original scientific paper

GENETIC DIVERSITY OF TURKISH Lathyrus L. LANDRACES USING ISSR MARKERS

Hüseyin UYSAL^1*, Zeki ACAR², İlknur AYAN², Orhan KURT²

¹Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydin, Turkey

²Ondokuz Mayis University, Agricultural Faculty, Department of Field Crops, Samsun, Turkey

Abstract

Uysal H., Z. Acar, İ. Ayan, O. Kurt (2018): Genetic diversity of Turkish lathyrus L. landraces using ISSR markers.- Genetika, Vol 50, No.2, 395-402.

Fifty-one Lathyrus sativus L. landraces and one L. clymenum L. landrace collected from Turkey and one L. sativus cultivar, Gürbüz, were evaluated with ISSR markers in this study to molecular characterization. Three ISSR primers were used and 45 DNA fragment were evaluated, of which 44 bands were polymorphic. The frequencies of scored bands ranged from 0.009 to 0.888 and averaged 0.363. The genotypes MLT04 and NEV02 had high similarity with 0.825 according to pairwise grouping. The furthest pairwise group was Gürbüz and MLT02 with 0.244. The nearest genotype to Gürbüz was CNR03 with 0.577. The pairwise genetic distance between L. clymenum and L. sativus ranged from 0.353 (accession NEV01) to 0.637 (accession DEN04) and pairwise genetic distance to the cultivar Gürbüz was 0.375. Assessment of genetic relationships among Lathyrus genotypes made two main groups. One of them covered only Gürbüz variety and the other covered 52 Lathyrus landrace. L. sativus and L. clymenum separated prominently in the second group.

Keywords: Fodder crop, Grass pea, ISSR, Lathyrus, genetic diversity

Corresponding author: Hüseyin Uysal, Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydin, Turkey, e-mail: hüseyin.uysal@adu.edu.tr

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 403-419

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802403E

Original scientific paper

MOLECULAR AND MORPHOLOGICAL STUDY OF THE GENUS Senecio L. (ASTERACEAE: SENECIONEAE) IN IRAN

Rosa EFTEKHARIAN^1*, Masoud SHEIDAI¹, Farideh ATTAR², Zahra NOORMOHAMMADI³, Somayyeh GHASEMZADEH-BARAKI¹

¹ Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran

²Faculty of Biological Sciences, Tehran University, Tehran, Iran

³ Department of Biology, School of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Eftekharian R., M. Sheidai, F. Attar, Z. Noormohammadi, S. Ghasemzadeh-Baraki (2018): Molecular and morphological study of the genus Senecio L. (Asteraceae: Senecioneae) in Iran.- Genetika, Vol 50, No.2, 403-419.

The genus Senecio (Asteraceae, Senecioneae) with about 1250 species is one of the largest genera in the family. Due to historical and present time inter-specific hybridization and reticulate evolution in the genus, the morphological and molecular phylogenetic evolution are disjunct. The genus contains 17 species belonging to four sections, sect. Crociseris, sect. Quadridentati, sect. Jacobaea and sect. Senecio, in Iran out of which, six are endemic. Therefore, the present study was performed with the aim to provide data on the above issues. In general, ISSR molecular markers could delimit the studied Senecio species and revealed the species relationships, but did not support any of the sections. The ITS and cp-DNA sequencing of six species of S. iranicus, S. vulcanicus, S. kotschyanus, S. paulsenii subsp. khorasanicus and S. joharchii were obtained for the first time. S-DIVA suggests three possible ancestral ranges, of Kordestan (A), Mazandaran (G), and West-Azarbayejan (D), for Sececio species in Iran. These areas are located in the western parts of Iran. Mazandaran province played important role in the speciation process that led to the formation of endemic Sececio species in the country, while, Kordestan and West-Azarbayejan are the main road for the entrance of Sececio species from Europe via neighboring countries. The morphological characters used could also delimit the four sections of the genus Senecio. However, ISSR, cp-DNA and nuclear genes sequences-based phylogenetic trees portrayed the species relationship much different from that of morphological dendrogram.

Keywords: cpDNA, divergence time, ISSR, ITS, Senecio sect. Crociseris, Senecio sect. Senecio, Senecio sect. Jacobaea, Senecio sect. Quadridentati

Corresponding author: Rosa Eftekharian, Faculty of Biological Sciences, Shahid Beheshti University, Shahid Chamran Highway, Tehran, Iran. Tel.: +98 21 29902111. E-mail: rozaeftekharian@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 421-430

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1802421M

Original scientific paper

EVALUATION OF RAPD MARKERS AS A MARKER-ASSISTED SELECTION TOOL FOR VARIETY TYPE AND ERUCIC ACID CONTENT IN RAPESEED

Dragana MILADINOVIĆ^1*, Marko MILER², Ana MARJANOVIĆ JEROMELA¹, Ivana IMEROVSKI¹, Aleksandra DIMITRIJEVIĆ¹, Branislav KOVAČEVIĆ³, Siniša JOCIĆ¹, Sandra CVEJIĆ¹, Nada HLADNI¹, Dragana OBREHT-VIDAKOVIĆ⁴

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Department of Cytology, Institute for Biological Research “Siniša Stanković“, University of Belgrade, Belgrade, Serbia

³Institute for Lowland Forestry and Environment, University of Novi Sad, Novi Sad, Serbia

⁴Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

Abstract

Miladinović D., M. Miler, A. Marjanović Jeromela, I. Imerovski, A. Dimitrijević, B. Kovačević, S.Jocić, S. Cvejić, N. Hladni, D. Obreht-Vidaković (2018): Evaluation of RAPD markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed.- Genetika, Vol 50, No.2, 421-430.

Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the position of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750.

Keywords: Brassica napus, cluster analysis, contingency analysis, molecular markers, erucic acid.

Corresponding author: Dragana Miladinović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad; E-mail: dragana.miladinovic@ifvcns.ns.ac.rs; Phone: 0214898421

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp.431-447

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802431M

Original scientific paper

DETERMINATION OF THE BEST MODEL FOR ESTIMATION OF GENETIC PARAMETERS ON THE FARS NATIVE CHICKEN TRAITS USING BAYESIAN AND REML METHODS

Ali MOHAMMADI*¹, Yousef NADERI², Reza NABAVI³

and Fatemeh JAFARI⁴

¹ Young Researchers and Elite Club, Hamedan Branch, Islamic Azad University, Hamedan, Iran

²Department of Animal Science, Astara Branch, Islamic Azad University, Astara, Iran

³Genetics and Animal Breeding, University of Tabriz, Tabriz, Iran

³Genetics and Animal Breeding, Ramin Agriculture and Natural Resources University, Khuzestan, Iran

Abstract

Mohammadi A., Y. Naderi, R. Nabavi and F. Jafari (2018): Determination of the best model for estimation of genetic parameters on the Fars native chicken traits using Bayesian and REML methods.- Genetika, Vol 50, No.2, 431-447.

This study was conducted to determine the best model for genetic parameter estimation on the Fars native chicken traits using Bayesian and REML Methods. Studied traits were body weight at first day (BW₁), body weight at eighth weeks (BW₈), body weight at 12th weeks (BW₁₂), age at sexual maturity (ASM), egg number production (EGP) and mean egg weight during 28^th,30^th and 32^nd week ages (EGW) involving three generations 17, 18 and 19 during the years 2010 to 2012. Genetic parameters were estimated with REML method using WOMBAT software and with Bayesian approach using MTGSAM software. Based on AIC and DIC criteria, the most appropriate model was determined. Estimations of direct additive heritabilities for BW₁, BW₈, BW₁₂, ASM, EGP and EGW by the best models using REML method were 0.31, 0.32, 0.29, 0.45, 0.24 and 0.22 and by Bayesian method were 0.36, 0.33, 0.30, 0.48, 0.26 and 0.25, respectively. The genetic correlation coefficients ranged from -0.709 between EGP and ASM to 0.844 between BW₈ and BW₁₂ (by Bayesian method) and ranged from -0.724 between ASM and EGP to 0.894 between BW₁₂ and BW₈ (by REML method). Generally, based on the employed criteria, the 1^st and 2^nd models can be suggested for analysis of body weight traits (BW₁, BW₈and BW₁₂), whereas for other traits (ASM, EGP and EGW), 1^st, 5^th, 4^th and 6^th models seems to be suitable for estimation of genetic parameters of the Fars Native fowls traits using Bayesian and REML Methods. The Bayesian approach recommended for estimation of genetic parameters on the Fars native chicken traits because this method used the prior distribution in the calculation process.

Key words: animal model, heritability, genetic correlation, gibbs sampling

Corresponding author: Ali Mohammadi; e-mail address: alimohammadi36@tabrizu.ac.ir; Tel.: +98 918 813 5839; fax: +98 813 450 6128.

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp.449-464

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1802449B

Original scientific paper

STABILITY ANALYSIS OF BREAD WHEAT LANDRACES AND LINES USING BIOMETRICAL GENETIC MODELS

Fatemeh BAVANDPORI^*, Jafar AHMADI, Sayyed Mohssen HOSSAINI

Department of Crop production and Breeding, Imam Khomeini International University, Iran

Abstract

Bavandpori F., J. Ahmadi, S. Mohssen Hossaini (2018): Stability analysis of bread wheat landraces and lines using biometrical genetic models.- Genetika, Vol 50, No.2, 449-464.

In order to evaluate yield stability of twenty genotypes of bread wheat, an experiment was conducted in randomized complete block design (RCBD) with three replications under irrigated and rainfed conditions in Razi University of Kermanshah for three years (2011-2013). Combined analysis of variance showed highly significant differences for the GEI. Stability determined by AMMI analysis indicated that the first two AMMI model (AMMI1–AMMI2) were highly significant (P<0.01). The GEI was three times higher than that of the genotype effect. The results of Biplot AMMI₂showed that, genotypes WC-47359, WC-47472, WC-4611, WC-47388 and WC-47403 had general adaptability. Based on the ASV and GSI, the genotypes number WC-47403 and WC-47472 revealed the highest stability. GGE biplot analysis of yield displaying main effect G and GEI justified 57.5 percent of the total variation. The first two principal components (PC1 and PC2) were used to create a 2-dimensional GGE biplot and explained 34.3, 23.2 of GGE sum of squares (SS), respectively. Genotypes WC-47403, PISHGAM2 exhibited the highest mean yield and stability. Based on the results obtained the best genotypes were WC-47403, PISHGAM2, WC-4968, WC-47472 and WC-47528 for breeding programs.

Key words: Adaptability, AMMI and GGE biplot analyses, Genotype × Environment Interaction, Yield Stability

Corresponding author: Fatemeh Bavandpori, Department of Crop production and Breeding, Imam Khomeini International University, Iran, E-mail: f.bavandpori@yahoo.com, Telephone and fax numbers: +989123128278, +982833780073

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 465-482

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1802465B

Original scientific paper

DIVERSITY OF LACTIC ACID BACTERIA ISOLATED FROM TRADITIONAL MONTENEGRIN DAIRY PRODUCTS

Mirjana BOJANIC RASOVIC¹, Sigrid MAYRHOFER²*, Mary A.A. OCHOME², Erna AJANOVIC¹, Marija ZUNABOVIC², Aleksandra MARTINOVIC³, Konrad J. DOMIG²

¹University of Montenegro, Biotechnical faculty, Podgorica, Montenegro

²BOKU - University of Natural Resources and Life Sciences, Vienna, Department of Food Science and Technology, Institute of Food Science , Vienna, Austria

³University of Donja Gorica, Faculty of Food Technology, Food Safety and Ecology,

Donja Gorica, Podgorica, Montengro

Abstract

Bojanic Rasovic M., S. Mayrhofer, M. A.A. Ochome, E. Ajanovic, M. Zunabovic, A. Martinovic, K. J. Domig (2018): Diversity of lactic acid bacteria isolated from traditional Montenegrin dairy products.- Genetika, Vol 50, No.2, 465-482.

Traditional production of fermented dairy products in Montenegro is carried out without adding defined starter cultures. This way of production involves lactic acid bacteria (LAB) that are normally present in the raw milk and production environment. This autochthonous (''wild'') fermentation microbiota represents a reservoir of unknown strains. In order to study the LAB diversity, 25 indigenous dairy products in Montenegro have been tested. Isolation was performed on microbial media M17 and MRS agar with or without supplementations under aerobic and anaerobic conditions at temperatures of 30°C, 37 °C and 44°C. Identification of these isolates at species level was done by species-specific PCR and gene regions sequencing of representatives of each RAPD-cluster. RAPD-PCR was used to characterize the isolates at strain level. Nine Lactobacillus species, five Leuconostoc species, four Enterococcus species as well as strains of the species Lactococcus lactis, Pediococcus pentosaceus and Streptococcus thermophilus were detected. It can be concluded that a rich lactic acid bacteria diversity existed in the analyzed Montenegrin dairy products. Further examination of the isolates could lead to the development of autochthonous starter cultures that would contribute to a product that is characteristic for the geographical area and to which the local population is accustomed.

Key words: lactic acid bacteria, Montenegrin cheese, diversity

Corresponding author: Sigrid Mayrhofer, BOKU – University of Natural Resources and Life Sciences, Vienna, Department of Food Science and Technology, Institute of Food Science; Muthgasse 18, A-1190 Vienna, Austria, Tel.: +43 1 47654 75455; fax: +43 1 47654 75459.

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 483-494

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802483A

Original scientific paper

GENETIC DIVERSITY OF JUJUBE (Ziziphus mauritiana) CULTIVARS

Wajeeha AMIN¹, Sajjad HUSSAIN¹, Muhammad Akbar ANJUM¹, Shaghef EJAZ¹,

Muhammad SAQIB¹, Muhammad Fasih KHALID¹, Sezai ERCISLI^2*, Shakeel AHMAD³

¹Department of Horticulture, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

²Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey

³Department of Agronomy, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan

Abstract

Amin W., S. Hussain, M. Akbar Anjum, S.Ejaz, Muhammad Saqib, M. Fasih Khalid , S. Ercisli, S. Ahmad (2018): Genetic diversity of jujube (Ziziphus mauritiana) cultivars).- Genetika, Vol 50, No.2, 483-494.

The present study was conducted on five jujube (Ziziphus mauritiana Lamk.) cultivars (Anokhi, Karela, Dill bahar, Ajooba and Dehli white) grown in two locations in Punjab, Pakistan during seasons 2014-2015 and 2015-2016. The aim of the study was to determine genetic diversity based on fruit properties fruit (weight, length, diameter), seed (weight, length, diameter), pulp weight, juice pH, total soluble solids (TSS), titratable acidity (TA), vitamin C, sugars (total, reducing, and non-reducing), antioxidant capacity and total phenolics. All cultivars significantly differed from each other in their fruit physico-chemical attributes. Dehli white cultivar had the highest fruit weight (22.90 g), fruit diameter (37.58 mm), pulp weight (16.83 g) and seed diameter (10.33 mm) than other cultivars. Dill bahar had the highest fruit length (43.75 mm), seed weight (2.69 g), juice pH (4.98), vitamin C content (55.86 mg/100 mL juice) and total phenolic contents (146.98 µg GAE per mL). Anokhi had the highest seed length (26.13 mm), non-reducing sugars (37.81%), total sugars (47.23%) and antioxidant capacity (1016.8 mM trolox per 100 mL). In Bahawalpur and Faisalabad’s environmental conditions Dehli white and Dill bahar cultivars were with better physical properties. However, according to biochemical properties Ajooba and Anokhi cultivars were better in Bahawalpur.

Key words: Environmental conditions, Fruit characteristics, Locations, Z. mauritiana

Corresponding author: Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: 090 4422312599, Fax: 090 4422360958, e-mail: sercisli@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 495-502

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802495M

Original scientific paper

SELECTION OF GARDEN ROSE CULTIVARS FOR USE AS A CUT FLOWER

Emina mladenović¹ Jelena čukanović¹, Biljana božanić-tanjga², Lazar pavlović¹,

Ksenija hiel¹, Mirjana mirić²

¹Department of Fruit growing, Viticulture, Horticulture and Landscape Architecture, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

²Pheno Geno Roses d.o.o. Novi Sad, Serbia

Abstract

mladenović E., J. čukanović, B. božanić-tanjga, L. pavlović, K. hiel, M. mirić (2018): Selection of garden rose cultivars for use as a cut flower.- Genetika, Vol 50, No.2, 495-502.

Efficacy of preservative solutions on vase life of garden roses has not been researched before. Vast variability and morphological characteristics of this group of roses are very important, making them suitable for their use in bouquets, arrangements and vases. This research was carried out to examine the influence of five preservative solutions on vase life of garden rose cut flowers. The aim of research was to determine best preservative solution for prolonging of vase life of garden rose cut flower. The experiment included 8 rose cultivars cultivated for garden use. Each treatment consisted of 10 cut garden roses. The cut garden rose flowers with vase solution containing Al₂(SO₄)₃+ethanol+sucrose register longer vase life and higher values in water uptake. Vase life of flowers held in tap water (control) was lowest (4.38 days). This research backs the assumption that with the use of preservative solutions, garden rose also can be used as a cut flower.

Key words: cut flower, garden roses, postharvest, preservative solutions, vase life

Corresponding author: Emina Mladenović, University of Novi Sad, Faculty of Agriculture, Sq. Dositeja Obradovića 8, 21000 Novi Sad, Serbia, Phone: +381 21 485 3269, e-mail: eminam@polj.uns.ac.rs

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 503-517

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1802503W

Original scientific paper

MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS

OF ONE OMEGA-GLIADIN GENE FROM Aegilops speltoides L.

Wanqing WANG^1,4, Ke WANG^2,4, Xi CHEN¹, Slaven PRODANOVIC³^*,

Xiaohui LI¹, Xingguo YE^2*, Yueming YAN^1*

¹College of Life Science, Capital Normal University, Beijing, China

²Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

³Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

⁴Contributed equally to this work

Abstract

Wang W., K. Wang, X. Chen, S. Prodanovic, X. Li, X.Ye, Y. Yan (2018): Molecular characterization and phylogenetic analysis of one omega-gliadin gene from Aegilops speltoides L..- Genetika, Vol 50, No.2, 503-517.

Gliadins, as the major components of wheat storage proteins, determine the extensibility properties of dough and have important effects on flour processing quality. Wheat related species carries potential storage protein gene resources for quality improvement. In this study, we isolated and characterized the first complete ω-gliadin gene Omega-AS from Aegilops speltoides L. (2n = 2x = 14, SS) by allelic-specific PCR and investigated its phylogenetic relationships among Triticum and Aegilops species. Molecular structure showed that Omega-AS gene consisted of 1122 bp encoding 373 amino acid residues with deduced molecular mass 41379.21 Da. Omega-AS gene was exceptionally rich in prolines and glutamines with fewer methionine and no cysteine. Sequence characterization and epitope analysis showed that three epitopes QQPIPVQPQQ, TQPQQPTPIQ and IQPQQPFPQQ were absent in Omega-AS gene encoded protein, indicating its potential value for wheat quality improvement with less toxic, or no toxic peptides . Phylogenetic analysis revealed that Omega-AS was closely related to gliadin genes of wheat and related species and its divergence from bread wheat was more recently (less than 1.243 MYA). Heterologous expression showed that Omega-AS gene could successfully express with a high level in E. coli under the control of T₇ promoter. The transcription expression pattern of Omega-AS gene during grain development detected by qRT-PCR revealed that the highest expression level occurred at 17 days post anthesis.

Key words Aegilops speltoides, ω-gliadins, phylogenetics, epitopes, heterologous expression

Corresponding author: Prof. Dr. Xingguo Ye, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China, Phone/fax: +86-10-82105173, E-mail: yexingguo@caas.cn

Prof. Dr. Yueming Yan, College of Life Science, Capital Normal University, Beijing, XisanhuanBeilu 105, 100048 Beijing, P.R. China, Phone/fax: +86-10-68902777, E-mail: yanym@cnu.edu.cn

Dr. Slaven Prodanović, Poljoprivredni fakultet, Univerzitet u Beogradu, Nemanjina 6, 11080, Beograd, Srbija, Phone: +381638687246, Fax: +381113161987, e-mail: slavenp@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 519-532

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802519G

Original scientific paper

MOLECULAR TAXONOMY AND PHYLOGENETICS OF Daedaleopsis confragosa (Bolt.: Fr.) J. Schröt. FROM WILD CHERRY IN SERBIA

Vladislava GALOVIĆ¹*, Miroslav MARKOVIĆ¹, Predrag PAP¹, Martin MULETT⁴, Milana RAKIĆ², Aleksandar VASILJEVIĆ³, Saša PEKEČ¹

¹University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia

²University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia

³Gljivarsko društvo Novi Sad, Novi Sad, Serbia

⁴Centre for Forestry and Climate Change (Centre for Forestry and Climate change Forest Research, Alice Holt Lodge Farnham Surrey GU104LH UK

Abstract

Galović V., M. Marković, P. Pap, M. Mulett, M. Rakić, A. Vasiljević, S. Pekeč (2018): Molecular taxonomy and phylogenetics of Daedaleopsis confragosa (Bolt.: Fr.) J. Schröt. from wild cherry in Serbia.- Genetika, Vol 50, No.2, 519-532.

Daedaleopsis spp., a lignicolous fungus causes of white rot on wild cherry and other broadleaved species and makes economic losses in Serbian forestry. The paper presents results of two morphologically distinct fungi Daedaleopsis confragosa and Daedaleopsis tricolor isolated from native populations of wild cherry (Prunus avium L.) found in the sites of Protected Forests of Serbia. Morphological appearance of D. tricolor was found more abundant in comparison to D. confragosa species. Samples from Serbia were analysed using morphometric and molecular tools and compared with isolates from United Kingdom and published sequences from Sweden, Austria, Hungary, Germany, Canada, France, USA and Czech Republic to give the taxonomic insight and their genetic relatedness using fungal barcoding region ITS rDNA. Results from BLAST search confirmed morphology of the isolates to their taxonomic affiliation as D. confragosa while sequence analysis showed mutations at several polymorphic positions that indicates genetic divergence to D. tricolor. Phylogenetic analysis presents narrow genetic relations of Serbian isolates with the one from United Kingdom while distinctness from other countries investigated specimens. Future work needs variable regions for both species to be amplified in order to evaluate species boundaries or employing NGS technologies in more detailed sequence analyses.

Key words: Prunus avium, Daedaleopsis confragosa, ITS rDNA, molecular taxonomy, phylogenetics

Corresponding author: Vladislava Galović, University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13d, 21000 Novi Sad, Serbia, Phone: +381/21540383, E-mail: galovic@uns.ac.rs;

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 533-548

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1802533A

Original scientific paper

BIOSYSTEMATIC STUDY AND POPULATION GENETIC ANALYSIS IN

Lamium amplexicaule L. (LAMIOIDEAE, LAMIACEAE)

Fatemeh AZIMISHAD¹, Masoud SHEIDAI¹*, Seyed Mehdi TALEBI²,

Zahra NOORMOHAMMADI³

¹Faculty of Life Sciences & Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

²Department of Biology, Faculty of Life Sciences, Arak University, Arak 38156-8-8349, Iran

³Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Azimishad F., M. Sheidai, S. Mehdi Talebi, Z. Noormohammadi (2018): Biosystematic study and population genetic analysis in Lamium amplexicaule L. (Lamioideae, Lamiaceae).- Genetika, Vol 50, No.2, 533- 548.

L. amplexicaule L. grows in different regions of Iran and forms several populations that can be invasive in some area. Morphological and genetic variation of L. amplexicaule in Iran was studied to evaluate the differentiation among three presumed varieties within this species. Morphometric study was accompanied by the analysis of ISSR and chloroplast genome (psbA-trnH). Results of the molecular methods show that L. amplexicaule var. bornmuelleri Mennema. does not form any genetically distinct group. Genetic study of 103 plant specimens of 13 different populations using ISSR molecular marker revealed a high degree of within-population (67%) and among (33%) populations genetic diversity. AMOVA and Gst analyses produced a significant difference. Nm estimation revealed certain degree of gene flow/shared alleles between these populations, indicating that L. amplexicaule is predominantly an out-crossing species in Iran. Mantel test produced significant correlation between genetic distance and geographical distance of the studied populations (r = 0.097, P = 0.04). STRUCTURE plot identified two main gene pools for this species in Iran.

Keywords: chloroplast genome, genetic diversity, gene flow, ISSR, L. amplexicaule

Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Department of Plant Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

E-mail: msheidai@yahoo.com, tel : +98 2129902111

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 549-560

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802549N

Original scientific paper

VARIABILITY AND HEREDITY OF SOME QUALITATIVE AND QUANTITATIVE GRAPEVINE CHARACTERISTICS

Dragan NIKOLIĆ¹*, Tijana BANJANIN², Zorica RANKOVIĆ-VASIĆ¹

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²University of East Sarajevo, Faculty of Agriculture, Bosnia and Herzegovina

Abstract

Nikolić D., T. Banjanin, Z. Ranković-Vasić (2018): Variability and heredity of some qualitative and quantitative grapevine characteristics.- Genetika, Vol 50, No.2, 549-560.

Variability and mode of heredity of some important qualitative and quantitative grapevine characteristics in 45 seedlings of F₁ generation obtained from crossing combination Seedling 113 x Muscat Hamburg were investigated in this study. The seedlings of F₁ generation for the investigated characteristics were arranged in certain number of categories by the OIV method. As variability indexes, standard deviation (S) and coefficient of variation (V) were used. Evaluation of the mode of heredity was done by test and t-test. Considering examined characteristics, the highest variability showed grape yield (V=58.9%), and the lowest sugar content in must (V=16.3%). Color of berry skin, cluster resistance to Botrytis cinerea and leaf resistance to Plasmopara viticola showed monogenic mode of heredity. Exception from the monogenic mode of heredity was determined for the flavor of berry. Grape yield, bunch weight and berry weight showed negative heterosis. For the sugar content in must, domination of the parent with low sugar content in must was determined.

Keywords: grapevine, heredity, F₁ generation, interspecies hybridization, variability

Corresponding author: Dragan Nikolić, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: +381 2615 315, fax: +381 2193 659, e-mail: nikolicd@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 561-574

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802561H

Original scientific paper

Evaluatıon OF dry matter accumulatıon IN trıtıcale by dıfferent sıgmoıdal growth models ın West Anatolıa of Turkey

Onur HOCAOĞLU¹, Yalçın COŞKUN²

¹Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Agricultural Structures and Irrigation, 17100 Çanakkale, Turkey

²Çanakkale Onsekiz Mart University, Lapseki Vocational College, Çanakkale, Turkey

Abstract

Hocaoğlu O., Y. Coşkun (2018): Evaluation of dry matter accumulation in triticale by different sigmoidal growth models in west Anatolia of Turkey.- Genetika, Vol 50, No.2, 561-574.

Monitoring biological growth of field crops is important for planning and timing agricultural practices. In order to assess biological growth pattern of dry matter accumulation in triticale Egeyildizi triticale variety were grown in Çanakkale conditions in 2012-2013 and 2013-2014 growing seasons with continuous plant samplings from seedling emergence until seed maturation. Gompertz, Logistic, Logistic Power and Richards growth models are fitted to actual growth data and their predictions were compared. Results suggested that all sigmoidal growth models successfully explained triticale dry matter accumulation over 98 % R²values and low mean square errors, Richards model fitted best for both years with an R²value over 99 %. Dry matter accumulation were also investigated as a result of average temperature, precipitation, growth degree days and cumulative growth degree days with stepwise regression. Rresults indicated that average weather temperature had a similar pattern across both growing seasons and had a major influence on dry matter accumulation. Since Richards sigmoidal growth model may be adequately described growth pattern of triticale by generally high R² with lower Mean Square Error (MSE) values.

Key words: Dry Matter, Growth Models, Stepwise Regression, Triticale

Corresponding author: Yalçın Coşkun, Çanakkale Onsekiz Mart University, Lapseki Vocational College, 17800 Çanakkale, Turkey, Phone: +90 286 522 61 04, Fax: +90 286 522 61 01,e-mail: ycoskun33@hotmail.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp.575-590

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1802575B

Original scientific paper

GENETIC RELATIONSHIP AMONG INTRODUCED LENTIL GERMPLASM USING AGRONOMIC TRAITS AND ISSR MARKERS

Sevda BABAYEVA¹, Zeynal AKPAROV¹, Litfer AMIROV^1,2, Kamila SHIKHALIYEVA¹, Saida HASANOVA¹, Khanbala RUSTAMOV¹, Rufat MIRZAYEV², Vusala IZZATULLAYEVA¹, Ilhama MIRZALIYEVA¹, Afig MAMMADOV¹, Mehraj ABBASOV^{1, 3}

¹Genetic Resources Institute of ANAS, Baku, Azerbaijan

²Azerbaijan Crop Husbandary Institute, Baku, Baku, Azerbaijan.

³Baku State University, Baku, Azerbaijan

Abstract

Babayeva S., Z. Akparov, L. Amirov, K. Shikhaliyeva, S. Hasanova, K. Rustamov, R.Mirzayev, V. Izzatullayeva, I. Mirzaliyeva, A. Mammadov, M. Abbasov (2018): Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers.- Genetika, Vol 50, No.2, 575-590.

Lentil (Lens culinaris Medik.) is an annual, cool-season grain legume playing an important role in human and animal nutrition, as soil fertility maintenance. National lentil improvement program in Azerbaijan is currently focused on extending the genetic base of the lentil collection through the introduction of new breeding lines from ICARDA and involving them into breeding. The present study was aimed to evaluate the performance of lentil collection, mainly comprised of ICARDA-derived breeding lines for yield traits under Azerbaijan condition and assess genetic diversity among them using inter simple sequence repeats (ISSR) markers. Many breeding lines of ICARDA exhibited agro-morphological performance superior to those of the local improved varieties. Our studies confirmed that the genetic base of the studied lentil collection is quite above board. A total of 71 bands were generated using 7 ISSR primers in 47 lentil genotypes, of which 62 were polymorphic. Genetic diversity values varied from 0.61 (UBC 848) to 0.95 (UBC 835), with a mean of 0.81. ISSR dendrogram was able to clearly distinguish all lentil accessions. Clear tendention was observed on clustering of genotypes according to their pedigree or origin with few exeptions. The results obtained from the Principal Coordinate Analysis were consistent with the results of cluster analysis, with minor differences. Breeding lines with high agronomic performance and sufficient genetic distance from this study can be used as appropriate parents to get more heterotic recombinants. This will accelerate the creation of new varieties well adapted to eco-geographic condition of Azerbaijan with stable and high yield.

Key words: genetic diversity; ISSR; lentil; yield components

Corresponding author: Mehraj Abbasov, Genetic Resources Institute of National Academy of Sciences of Azerbaijan, Azadliq Ave 155, AZ1106, Baku, Azerbaijan, e-mail: mehraj_genetic@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 591-602

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1802591M

Original scientific paper

Agronomic performance of wheat cultivars and their molecular characterization

Velimir MLADENOV¹, Miodrag DIMITRIJEVIĆ¹, Sofija PETROVIĆ¹,

Jan BOĆANSKI¹, Ankica KONDIĆ-ŠPIKA², Dragana TRKULJA², Borislav BANJAC¹*

¹University of Novi Sad, Faculty of Agriculture Novi Sad

²Institute for Field and Vegetable Crops, Novi Sad

Abstract

Mladenov V., M. Dimitrijević, S. Petrović, J. Boćanski, A. Kondić-Špika, D. Trkulja, B. Banjac (2018): Agronomic performance of wheat cultivars and their molecular characterization.- Genetika, Vol 50, No.2, 591-602.

Creation of new higher yield cultivars, adaptation of existing germplasm to a global climate change, increasing resistance to diseases in new genotypes are some of the tasks that breeding have in front of it. The objectives of this research were to assess GE interaction in two different environments across two vegetation seasons and to do association analysis based on the results of the phenotypic and molecular evaluation. Grain samples were obtained from 96 winter wheat cultivars grown in 2011/12 and 2012/13 at two locations in the South Pannonia Basin region and population was profiled with 28 microsatellites. The share of genotype is high and amounts 24.84%, while the share of environments was 21.06%, when yield was evaluated. The GE interaction was also statistically significant and amounts 51.58% of the total variance. Microsatellites that exhibited a relation with yield by GLM and MLM model were: gwm357, gwm339, cfa2114, gwm631, gwm495, gwm190, barc1121 and gwm437. Markers that have demonstrated the stability of the relationship with yield in different environments can be recommended as potentially useful in wheat breeding.

Keywords: AMMI, GE interaction, microsatellites, yield, wheat.

Corresponding author: Borislav Banjac, University of Novi Sad, Faculty of Agriculture Novi Sad, Sq. Dositeja Obradovića 8, 21 000 Novi Sad, phone: +381 (21) 485 32 99; e-mail:borislav.banjac@polj.edu.rs

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 603-616

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802603S

Original scientific paper

GENOME-WIDE IDENTIFICATION AND ANALYSIS OF RUBISCO LARGE SUBUNIT PROTEINS IN Morus L. (MORACEAE) SPECIES

Emre SEVİNDİK

Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, Aydin, Turkey

Abstract

sevindik E. (2018): Genome-wide identification and analysis of rubisco large subunit proteins in Morus L. (Moraceae) species.- Genetika, Vol 50, No.2, 603-616.

In this study, we performed sequence, physicochemical, phylogenetic, and comparative 3D (three-dimensional) analyses of RuBisCO large subunit proteins of the Morus species using various bioinformatics tools. The sequence lengths of the RuBisCO proteins varied between 140 and 475 amino acids with molecular weights (MW) ranging from 15463.40 to 52667.95 Da. The most acidic protein sequences were in M. australis, M. rotundiloba, M. serrata, and M. cathayana (pI=4.77). The extinction coefficients of the proteins at 280 nm ranged from 21430 to 69830 M^-1 cm^-1, the instability index (II) values ranged from 26.06 to 42.17, and the GRAVY values ranged from -0.355 to -0.215. The most abundant amino acid of RuBisCO proteins was Gly (9.5%) while the least abundant ones were Cys and Trp (1.5%). Based on the phylogenetic analysis, the tree constructed using RuBisCO proteins is composed of three main clades. A RAMPAGE analysis revealed that 96.1% - 98.5% of residues were located in the favored region in RuBisCO proteins. To predict the three dimensional (3D) structures of the RuBisCO proteins PyMOL was used. The highest number of Gly residues was detected in M.mongolica, M. indica, M.notabilis, M. alba var. atropurpurea and M. alba var. multicaulis, while the least Gly residues were detected in M. australis, M.rotundiloba, M. serrata and M.cathayana. The results of our study provide insights into fundamental characteristic of RuBisCO proteins in Morus species.

Key words: genome-wide analysis, Morus, phylogenetics, RuBisCO

Corresponding author: Emre sevindik,Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey, e-mail: ph.d-emre@hotmail.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2017), pp. 617-626

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802617F

Original scientific paper

ASSOCIATION ANALYSIS BETWEEN GENETIC DIVERSITY

OF THE MELANOCORTIN4 RECEPTOR (MC4R) GENE AND PRODUCTION TRAITS IN TURKEY

Mehrangiz FATHI¹, Ali HASHEMI^1*, Ghorban ELYASEI ZARRIN GHOBAYI²

¹Department of Animal Science, Faculty of Agriculture Science, University of Urmia, Iran

²Department of Agriculture Research Center of East Azerbaijan, Tabriz, Iran

Abstract

Fathi M., A.Hashemi, G. Elyasei Zarrin Ghobayi (2018): Association analysis between genetic diversity of the melanocortin4 receptor (MC4R) gene and production traits in Turkey.- Genetika, Vol 50, No.2, 617-626.

Melanocortin receptor (MC4R) plays an important role in central melanocortin system and regulating feeding behavior in humans and birds. The current study was designed to estimate the frequency of MC4R gene polymorphism and investigate if their polymorphisms have association with production traits Turkeys (Meleagris gallopavo) by PCR-SSCP method. For this purpose, a number of 100 turkeys were randomly chosen and blood samples were collected. DNA were extracted from blood samples, and amplified a fragment of 469 bp in size from MC4R gene. Single strand conformation polymorphism technique (SSCP) was used to determine genotypes. Electrophoresis of SSCP-PCR products on 6% polyacrylamide gel indicated that the polymorphism of the MC4R gene. Also, results of electrophoresis showed that three genotypes AA, AB and BB, with frequencies of 0.2397, 0.4998 and 0.2605, respectively. Shanon index, Nei index and observed heterozygosity with frequencies of 0.6929, 0.4998 and 0.5208, respectively.Significant associations (P<0.01) were obtained for genotypes with live body weight. But no significant relationship between the genotypes with egg performance (egg mass, mean egg weight and the number of eggs) is found. These results suggest that, the turkey MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight, and at future more study is required to confirm these results in Turkey.

Key words: MC4R, Turkey, Polymorphism, Production Traits

Corresponding author: Ali Hashemi, Department of Animal Science, Faculty of Agriculture Science, University of Urmia, Iran, e-mail a.hashemi50@gmail.com, Tel:091434411702

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 627-634

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802627F

Original scientific paper

GENETIC FINGER PRINTING OF COTTON CULTIVARS BY ISSR MOLECULAR MARKERS

Farah FARAHANI^1*, Masoud SHEIDAI², Fahimeh KOOHDAR²

¹Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

² Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract

Farahani F., M. Sheidai, F. Koohdar (2018): Genetic finger printing of cotton cultivars by ISSR molecular markers.- Genetika, Vol 50, No.2, 627-634.

Gossypium hirsutum is one of the main tetraploid cotton species that is cultivated throughout the world. Due to continuous selection of cotton cultivars for specific agronomic traits, the genetic variability within the cultivars decrease that lead to genetic erosion. To tackle the problem of reduced genetic variability, we should track all available genetic diversity within cotton germplasm and use them for inter-specific and intra-specific hybridization and produce new elite cotton cultivars. Therefore, the present study used ISSR molecular markers to illustrate genetic variability in 13 tetraploid cotton genotypes (Gossypium hirsutum L.) and to categorize these genotypes based on genetic affinity. 65 cotton plants were studied. The results identified private bands in the studied genotypes, while Network and STRUCTURE analyses of molecular data obtained grouped the genotypes with genetic affinity together. Some of the genotypes differed in their genetic content from the others; therefore, studying the genetic and agronomic variability within available cultivars is very important and produced data to broaden the gene pool for planning further hybridization in cotton.

Keywords: Cotton, genetic variability, ISSR, Network, STRUCTURE analysis

Corresponding author: Farah Farahani, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.E-mail: farahfarahani2000@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp.635-646

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1802635U

Original scientific paper

ANALYSIS OF SPELT VARIABILITY (Triticum spelta L.) GROWN IN DIFFERENT CONDITIONS OF SERBIA BY ORGANIC CONDITIONS

Vladan ugrenović^1*, Marija bodroža solarov², Lato pezo^*3, Jovana đisalov², Vera popović⁴, Boško marić², Vladimir filipović⁵

¹Institute „Tamiš” Pančevo, Serbia

²Institute of Food Tehnology, Novi Sad, Serbia

³Institute of General and Physical Chemistry, University of Belgrade, Belgrade, Serbia

⁴Institute of Field and Vegetable Crops, Novi Sad, Serbia

⁵Institute for Medicinal Plants Research „Dr Josif Pančić”, Serbia

Abstract

ugrenović V., M. bodroža solarov, L. pezo, J. đisalov, V. popović, B. marić, V. filipović (2018): Analysis of spelt variability (Triticum spelta L.) grown in different conditions of Serbia by organic conditions.- Genetika, Vol 50, No.2, 635-646.

The objective of this study was to examine the effect of the variety, locality and year on the morphological and productive parameters of the spelt wheat obtained in the organic production system. Four spelt varieties were grown at three agroecological environment (Pančevo, Bogdanica and Zlatar), in southeast Europe, Serbia. They are used in order to determine general adaptability in different environment in three years trial (2014-2016). Analysis of the selected production parameters of spelt wheat, according to variety, locality and year, were conducted by the Principal Component Analysis (PCA). The PCA of the presented data explained that the first two components accounted 86.02% of the total multivariance in the eleven variable factor space. The results showed that the highest values of a number of plants, number of stems, number of spikes, plant height,the number of grains in the spikes, weight of grains per spike, yield of above ground biomass, harvest index and yield of hulled grain were observed for the crops grown in Pančevo, regardless of the spelt wheat variety and year. The samples grown in Bogdanica showed to be very sensitive to the spelt wheat variety, in terms of hull index value. The highest value of mortality stems has been found in Zlatar, regardless of the spelt wheat variety, or the year.

Keywords: Mathematical modelling, morphological and productive traits, Triticum spelta, varieties, year.

Corresponding author: Vladan Ugrenović, Institute "Tamis" Pančevo, Serbia (e-mail: vladan.ugrenovic@gmail.com) and dr Lato Pezo Institute of General and Physical Chemistry, University of Belgrade, Belgrade, Serbia, (e- mail: latopezo@yahoo.co.uk)

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Published in „GENETIKA“ Vol. 50, No. 2(2018), pp. 647-657

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1802647S

Original scientific paper

EVALUATION OF THE EFFECT OF AMYGDALIN ON survivin GENE EXPRESSION IN MCF-7 BREAST CANCER CELL LINE

Iman SHAHROKHIYAN¹, Abbas DOOSTI^2*, Hossein SAZEGAR¹

¹Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

²Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Shahrokhiyan I., A. Doosti, H. Sazegar (2018): Evaluation of the effect of amygdalin on survivin gene expression in MCF-7 breast cancer cell line.- Genetika, Vol 50, No.2, 647-657.

The effects of amygdalin as an herbal substance on prevention of cancer cells proliferation it has been shown. Also, survivin gene is one of the important genes on inhibition of apoptosis and controlling of the cell cycle in cancer cell lines. For this purpose, the present study was undertaken for the first time to evaluate the effects of amygdalin on survivin gene expression in MCF-7 and HDF. The MCF-7 (Breast cancer cells) and normal HDF were treated by different doses of amygdalin (0.5 to 10 mg/ml). Then, the MTT assay was done on each cell groups on 24, 48, and 72 h after treatment. In each period time the cells were detached and used for RNA extraction and cDNA synthesis. Finally, the survivin gene expression in each cell groups was evaluated by quantitative real-time PCR (q-RT-PCR) analysis. The MTT assay was showed that 5 mg/ml of amygdalin concentration at 48 hours after treatment it was suitable for evaluation of this compound on MCF-7 and HDF cell lines. The survey results of q-RT-PCR were showed in both amygdalin-treated MCF-7 (MCF-7+AMG) compare to the treated HDF (HDF-AMG as an control group) the expression level of survivin gene were decreased but this reduction only in MCF-7+AMG group was statistically significant (p <0.05). Our findings indicated that amygdalin in particular; decrease the survivin mRNA in MCF-7 breast cancer cells compare to normal healthy cells and leads to apoptosis and the death of cancer cells. It is suggested that in future study it must be better the effects of amygdalin supplemented with surfactant investigate against other cancer cells and evaluate the more molecular markers to specify the anti-cancer activity potential of this herbal compound.

Key words: Breast cancer, Gene expression, HDF, MCF-7, Survivin

Corresponding author: Abbas Doosti, Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, Postal box: 166, E-mail: biologyshki@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp 659-668

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802659N

Original scientific paper

POLYMORPHISM OF K-CASEIN AND B-LACTOGLOBULINE IN SIMMENTAL CATTLE IN SERBIA

Dragan NIKŠIĆ¹, Vlada pantelić¹, Dušica ostojić-andrić¹,Predrag perišić²,

Veselin petričević¹, Marina lazarević¹, Maja petričević¹

¹Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia

²Faculty of Agriculture, University of Belgrade, Belgrade-Zemun, Republic of Serbia

Abstract

Nikšić D., V. pantelić, D. ostojić-andrić,P.perišić, V. petričević, M. lazarević, M. petričević (2018): Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia.- Genetika, Vol 50, No.2, 659-668.

The objective of this study was to determine the frequency of the κ-casein and β-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA κ-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and β-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for β-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population.

Key words: β-lactoglobulin, κ-casein, polymorphism, PCR-RFLP, Simmental breed

Corresponding author: Dragan Niksic, Institute for Animal Husbandry, Autoput 16, 11080, Belgrade-Zemun, Republic of Serbia, e-mail: draganniksic84@gmail.com

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 669-686

UDC 575.630

https://doi.org/10.2298/GENSR1802669A

Original scientific paper

PRELIMINARY RESULTS OF TURKISH HAZELNUT (Corylus colurna L.) POPULATIONS FOR TESTING THE NUT CHARACTERISTICS

Sezgin AYAN^1*, Erkan ÜNALAN², Oytun Emre SAKICI¹, Esra Nurten YER¹,

Fulvio DUCCI³, Vasilije V. ISAJEV⁴, Halil Barıs OZEL⁵

¹Kastamonu University, Faculty of Forestry, Forest Engineering Department, Kastamonu, Turkey

²Kastamonu Directorate of Provincial Food Agriculture and Livestock, Kastamonu, Turkey

³Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria - Forestry and Wood Research Centre (CREA FL), Arezzo, Italy

⁴Institute for Forestry, Kneza Viseslava 3, Belgrade, Serbia

⁵Bartın University, Faculty of Forestry, Silviculture Department, Bartın, Turkey

Abstract

Ayan S., E. Ünalan, O. E. Sakıcı, E. N. Yer, F. Duccı, V. V. Isajev, H. B. Ozel (2018): Preliminary results of Turkish hazelnut (Corylus colurna L.) populations for testing the nut characteristics.- Genetika, Vol 50, No.2, 669-686.

This paper aims to identify the hazelnut characteristics of four different populations (Ağlı-Tunuslar, Ağlı-Müsellimler, Araç-Güzlük and Tosya-Küçüksekiler) in the North Western Black Sea Region of Turkey, one of the most important areas of economic interest for this species. There, the Turkish hazel (Corylus colurna L.) grows in its optimal conditions and reveals relatively high inter-population and intra-population variation in terms of nut characteristics. With the purpose of assessing variation, measurements were performed in four populations in Kastamonu district on 14 different nut characteristics (number of nuts per cluster, nut length (mm), nut width (mm), nut thickness (mm), shell thickness (mm), nut size (mm), nut shape, compression index, nut weight (g), kernel length (mm), kernel width (mm), kernel thickness (mm), kernel weight (g) and kernel ratio (%) of representative samples of the populations. Significant differences were found out among populations with regard to all of nut characteristics (p<0.05). The four populations have created two groups, population of Ağlı-Tunuslar and the others, according to cluster analysis. The closest populations have been Tosya-Küçüksekiler and Araç-Güzlük in terms of nut characteristics. According to the results obtained either on population basis or without population discrimination; significant correlations were determined between the majorities of the nut characters. The Araç-Güzlük population showed nuts the biggest among those examined and it is the population which took the highest values in terms of nut size traits while the Tosya-Küçüksekiler provenance showed the highest values with the average values of 5, 15.92 mm, 1.32 and 11.75 mm respectively for nuts per cluster, nut width, compression index and kernel width. The Ağlı-Tunuslar population showed the highest kernel ratio with 38.2%.

Key words: Corylus colurna L., Nut characteristics, Population diversity, Turkey Turkish hazel

Corresponding author: Sezgin Ayan, Kastamonu University, Faculty of Forestry, Forest Engineering Department, Kastamonu, Turkey, Phone: +90 366 280 1009 Fax: +90 366 280 1004, sezginayan@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 2 (2018), pp. 687-703

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1802687B

Original scientific paper

PURSUIT FOR EST MICROSATELLITES IN A TETRAPLOID MODEL FROM DE NOVO TRANSCRIPTOME SEQUENCING

Tijana Banjanac¹, Marijana Skorić¹, Mario Belamarić², Jasmina Nestorović Živković¹, Danijela Mišić¹, Mihailo Jelić², Slavica Dmitrović¹, Branislav Šiler¹

¹Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

²Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Banjanac T., M. Skorić, M. Belamarić, J. Nestorović Živković, D. Mišić, M. Jelić, S. Dmitrović, B. Šiler (2018): Pursuit for EST microsatellites in a tetraploid model from de novo transcriptome sequencing.- Genetika, Vol 50, No.2, 687-703.

Available scientific literature reports very few microsatellite markers derived from tetraploid genomes using de novo transcriptome sequencing, mostly because their gain usually represents a major computational challenge due to complicated combinatorics during assembly of sequence reads. Here we present a novel approach for mining polymorphic microsatellite loci from transcriptome data in a tetraploid species with no reference genome available. Pairs of 114 bp long de novo sequenced transcriptome reads of Centaurium erythraea were merged into short contigs of 170-200 bp each. High accuracy assembly of the pairs of reads was accomplished by a minimum of 14 bp overlap. Sequential bioinformatics operations involved fully free and open-source software and were performed using an average personal computer. Out of the 13 150 candidate contigs harboring SSR motifs obtained in a final output, we randomly chose 16 putative markers for which we designed primers. We tested the effectiveness of the established bioinformatics approach by amplifying them in eight different taxa within the genus Centaurium having various ploidy levels (diploids, tetraploids and hexaploids). Nine markers displayed polymorphism and/or transferability among studied taxa. They provided 54 alleles in total, ranging from 2 to 14 alleles per locus. The highest number of alleles was observed in C. erythraea, C. littorale and a hybridogenic taxon C. pannonicum. The developed markers are qualified to be used in genetic population studies on declining natural populations of Centaurium species, thus providing valuable information to evolutionary and conservation biologists. The developed cost-effective methodology provides abundant de novo assembled short contigs and holds great promise to mine numerous additional EST-SSR-containing markers for possible use in genetics population studies of tetraploid taxa within the genus Centaurium.

Key words: microsatellite mining, free and open-source software, tetraploid genome, Centaurium, polymorphism

Corresponding author: Tijana Banjanac, Institute for Biological Research “Siniša Stanković”, Bul. despota Stefana 142, Belgrade, Serbia; tel/fax: +381112078404; e-mail: tbanjanac@ibiss.bg.ac.rs; orcid.org/0000-0001-9023-9135

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Published in „GENETIKA“ Vol. 50, No. 2(2018), pp. 705-716

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802705P

Original scientific paper

INFLUENCE OF CADMIUM CHLORIDE ON THE FREQUENCY OF MICRONUCLEI IN DA AND AO RATS

Slavica POPOVIĆ BUBUJUK¹, Ninoslav DJELIĆ^2*, Adrian MUSTER³, Dragan KATARANOVSKI⁴, Marko ANDJELKOVIĆ⁵

¹Faculty of Biofarming, Megatrend University, BačkaTopola, Serbia

²Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

³Faculty of mechanical engineering, University of Belgrade, Belgrade, Serbia

⁴Faculty of Biology, University of Belgrade, Belgrade, Serbia

⁵Serbian Academy of Science and Arts, Belgrade, Serbia

Abstract

Popović Bubujuk S., N. Djelić, A. Muster, D. Kataranovski, M. Andjelković (2018): Influence of cadmium chloride on the frequency of micronuclei in DA and AO rats.- Genetika, Vol 50, No.2, 705-716.

Heavy metal cadmium (Cd), a well–known environmental hazard, exerts a number of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and Dark August (DA) rat strains, were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl₂): 0.5, 1, and 2 mg CdCl₂per kg of body weight, while the control animals received equal volume of sterile phosphate buffered saline. In this investigation individuals of both sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after treatment. The results showed that that cadmium-cloride (CdCl₂) exibits the genotoxic effects causing an increase of the frequency of micronuclei depending on concentration, sex, age and strain.

Keywords: Albino Oxford, cadmium, Dark August, micronucleus, rats

Corresponding author: Ninoslav Djelić, Department of Biology, Faculty of Veterinary Medicine University of Belgrade, Oslobodjenja Blvd. 18, Belgrade, Serbia, Phone: +381 11 36 71 721, Fax: +381 11 268 59 36, E-mail: ndjelic@vet.bg.ac.rs

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Published in „GENETIKA“ Vol. 50 No. 2 (2018), pp. 717-730

Serbia

UDC 575

https://doi.org/10.2298/GENSR1802717M

Original scientific paper

GENETIC RELATIONSHIP BETWEEN NEUROPTERAN FAMILIES (INSECTA, NEUROPTERIDA, NEUROPTERA) BASED ON CYTOCHROME OXIDASE-I SEQUENCES

Alinaghi MIRMOAYEDI^1*, Fatemeh RASHIDIKHAH¹, Danial KAHRIZI^2,4

Kheirullah YARI^3,4

Department of Plant Protection, Razi University, Kermanshah, Iran

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Zagros Bioidea Company, Razi University Incubator, Kermanshah, Iran

Abstract

Mirmoayedi A., F. Rashidikhah, D. Kahrizi, K. Yari (2018): Genetic relationship between neuropteran families (insecta, neuropterida, neuroptera) based on cytochrome oxidase-i sequences.- Genetika, Vol 50, No.2, 717-730.

The studied specimens belonged to five species of three families of Chrysopidae, Myrmeleontidae and Ascalaphidae (order Neuroptera). They were collected from Shush and Dezful, south of Iran. These insects are useful insects in biological control as the important predators of aphids, psyllids, caterpillars, ants and other insects. The Sequence alignment of parts of Cytochrome Oxidase-I (COI) gene of those species was studied. The insect bodies were entirely ground in a microtube. The PCR products of COI were sequenced. Pairwise alignment of nucleotides sequences belonging to five species of Neuroptera was carried out using MegAlign and EditSeq softwares. The sequencing results in Palpares solidus and Creoleon remanei showed the mutation potentials in locations of 699, 702, 726, 735 and 750 of COI gene of mtDNA. According to COI gene sequences, Chrysopa pallens and Palpares solidus species showed the maximum genetic similarity (98.2%). There was the minimum genetic similarity (75.9%) between Chrysopa viridana and Creoleon remanei species.

Key words: Cytochrome Oxidase-I (COI), Genetic Relationship, Neuroptera

Corresponding author: Alinaghi Mirmoayedi, Department of Plant Protection, Razi University, Kermanshah, alimrmoayedi@gmail.com, Tel: 09181317087

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 731-745

Serbia

UDC 575.https://doi.org/10.2298/GENSR1802731M

Review paper

c-Myc MISREGULATION TRIGGERS COMPLEX PROCESS OF GENOMIC INSTABILITY

Dragan marinković¹, Tatjana marinković²

¹Faculty for Special Education and Rehabilitation, University of Belgrade, Belgrade, Serbia

² Sanitary-Medical School of Applied Sciences VISAN, Belgrade, Serbia

Abstract

marinković D. and T. marinković (2018): c-Myc misregulation triggers complex process of genomic instability.- Genetika, Vol 50, No.2, 731-745.

Genetic stability is an essential factor for the cellular integrity. Failure in its maintenance leads to accumulation of errors derived from the process of DNA replication, cellular metabolism, action of endogenous and exogenous DNA damaging factors and eventually, as a final outcome tumor initiation and progression occur. Overall manifestation of c-Myc deregulation in many tumors and different mechanisms of Myc’s action toward genomic stability suggest that this gene plays a central role in destabilization of genome. Microarray studies and functional genomics approach led us to conclusion that c-Myc can control nuclear architecture in global fashion since about 15% of all cellular genes are regulated by this transcription factor. Deregulation of c-Myc gene triggers a composite network of genomic instability that may result in several different outcomes as: locus-specific amplification, formation of extrachromosomal elements (EEs), chromosomal instability, long-range illegitimate recombination, point mutations, DNA breakage and nuclear structure reorganization. This review outlines the growing evidence that c-Myc oncogene induces a complex network of genomic instability and describes systems and circumstances under which deregulation of c-Myc results in specific types of genomic alteration.

Keywords: c-Myc, genomic instability, DNA damage, chromosomal rearrangement, gene amplification.

Corresponding author: Dragan Marinković, Faculty for Special Education and Rehabilitation, University of Belgrade, Visokog Stevana 2, Belgrade, Serbia, e-mail: dragan.marinkovic@hotmail.com; tel. ++381 64 503 97 64

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Published in „GENETIKA“ Vol. 50, No.2 (2018), pp. 747-753

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1802747H

Short communication

EFFECT OF TERMINAL DROUGHT ON YIELD AND SOME PHYSIOLOGICAL TRAITS OF WINTER WHEAT

Ivanka HABUŠ JERČIĆ¹, Marijana BARIĆ¹, Snježana KEREŠA¹, Anita BOŠNJAK MIHOVILOVIĆ¹, Milan POLJAK², Boris LAZAREVIĆ²*

¹Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia

²Department of Plant Nutrition, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia

Abstract

Habuš Jerčić I., M. Barić, S. Kereša, A. Bošnjak Mihovilović, M. Poljak, B. Lazarević (2018): Effect of terminal drought on yield and some physiological traits of winter wheat. - Genetika, Vol 50, No.2, 747-753.

Terminal drought i.e. drought during grain-filling phase is the most devastating environmental stress to wheat production. In present study the effect of terminal drought on physiological traits and its influence on yield and yield components in two winter wheat varieties (Kuna and Karla) were investigated. Terminal drought stress was applied from the beginning of anthesis by installing mobile plastic roof above the crops. Leaf gas exchange parameters, chlorophyll content index (CCI), relative water content (RWC), and nitrogen (N) content were measured three times during grain-filling phase, at early milk maturity (EMM), late milk maturity (LMM), and at early wax maturity (EWM). Grain yield and 1000 grain weight were measured by harvesting of each plot at crop maturity. Terminal drought enhanced leaf senescence and caused reduction of RWC, CCI, net photosynthetic rate (A), stomatal conductance (g_s), and transpiration rate (E) as well as, grain yield and all measured yield components. However, grain yield and grain weight per ear were less affected in Karla indicating enhanced tolerance to terminal drought compared to Kuna variety. Higher tolerance to terminal drought in Karla is based on stay-green strategy. Stay-green strategy in Karla was characterized by retention of CCI at early wax maturity, which contributed to higher E and lower intercellular CO₂ concentration compared to Kuna under terminal drought. Stay-green strategy as trait that enhanced terminal drought tolerance in Karla should be used in breeding programs and utilized to ensure maximum economic yields under terminal drought conditions.

Key words: gas exchange; chlorophyll content; relative water content; stay-green strategy

Corresponding author: Boris Lazarević, Department of Plant Nutrition, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia, e-mail: blazarevic@agr.hr, tel.: +385 01 239 3961

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Published in „GENETIKA“ Vol. 50, No.2 (2018)

Serbia

Dragoslav Marinković

SERBIAN GENETIC SOCIETY (1968 – 2018)