GENETIKA, Vol

GENETIKA, Vol. 50, No. 3(2018)

Dragan BOŽOVIĆ, Tomislav ŽIVANOVIĆ, Vera POPOVIĆ, Mladen TATIĆ, Zagorka GOSPAVIĆ, Zoran Miloradović, Goran STANKOVIĆ, Milorad ĐOKIĆ

Assessment stability of maize lines yield by GGE-BIPLOT analysis [Abstract] [Full text]

Tanmay MUKHOPADHYAY, Soumen BHATTACHARJEE

ANALYSES OF GENETIC DIVERSITY OF BADIS BADIS (HAMILTON-BUCHANAN 1822) FROM THREE RIVERINE SYSTEMS IN SUB-HIMALAYAN BIODIVERSITY HOTSPOT OF WEST BENGAL, INDIA USING RAPD AND ISSR FINGERPRINTING [Abstract] [Full text]

Jasmin GRAHIĆ, Mirha ĐIKIĆ, Drena GADŽO, Silvio ŠIMON, Mirsad KURTOVIĆ, Ivan PEJIĆ, Fuad GAŠI

ASSESSMENT OF GENETIC RELATIONSHIPS AMONG COMMON BUCKWHEAT (Fagopyrum esculentum MOENCH) VARIETIES FROM WESTERN BALKANS USING MORPHOLOGICAL AND SSR MOLECULAR MARKERS [Abstract] [Full text]

Jaroslava OVESNÁ, Daniela RUSSO, Domenico FRESCURA, Eloy Fernández CUSIMAMANI, Eva SVOBODOVÁ, Luigi MILELLA

ASSESSMENT OF GENETIC DIVERSITY OF Smallanthus sonchifolius (Poepp. & Endl.) H. ROBINSON LANDRACES BY USING AFLP MARKERS [Abstract] [Full text]

Mirela MATKOVIC STOJSIN, Veselinka ZECEVIC, Sofija PETROVIC, Miodrag DIMITRIJEVIC, Danica MICANOVIC, Borislav BANJAC, Desimir KNEZEVIC

VARIABILITY, CORRELATION, PATH ANALYSIS AND STEPWISE REGRESSION FOR YIELD COMPONENTS OF DIFFERENT WHEAT GENOTYPES [Abstract] [Full text]

Daniela AVDJIEVA-TZAVELLA, Hadil KATHOM, Ivanka SINIGERSKA, Zlatina TASEVA, Radka TINCHEVA

RESULTS OF ENZYME REPLACEMENT THERAPY IN BULGARIAN PATIENTS WITH A SEVERE FORM OF HUNTER SYNDROME: A 42-MONTH FOLLOW-UP [Abstract] [Full text]

Parisa TAHMASEBI, Morteza Hashemzadeh CHALESHTORI, Fatemeh ABDOLLAHNEJAD, Zahra ALAVI, Ladan SADEGHIAN, Farah TALEBI, Javad MOHAMMADI-ASL, Nader SAKI, Seyed Reza Kazemi NEZHAD, Mohammad Amin TABATABAIEFAR
FREQUENCY OF GJB2 MUTATIONS IN FAMILIES WITH AUTOSOMAL RECESSIVE NON-SYNDROMIC HEARING LOSS IN KHUZESTAN PROVINCE [Abstract] [Full text]

Yumin CHI, Qingguo DI, Guangchao HAN, Min LI, Baohua SUN

ASSOCIATION BETWEEN IL-4 GENE POLYMORPHISMS AND CHRONIC OBSTRUCTIVE PULMONARY DISEASE OF PATIENTS IN HEBEI PROVINCE, CHINA [Abstract] [Full text]

Dragan STANOJEVIĆ, Radica DJEDOVIĆ, Vladan BOGDANOVIĆ, Nikola RAGUŽ, Denis KUČEVIĆ, Mladen POPOVAC, Petar STOJIĆ, Ljiljana SAMOLOVAC

GENETIC TREND OF FUNCTIONAL PRODUCTIVE LIFE IN THE POPULATION OF BLACK AND WHITE CATTLE IN SERBIA [Abstract] [Full text]

Suzana JORDANOVSKA, Zoran JOVOVIĆ, Željko DOLIJANOVIĆ, Vesna DRAGIČEVIĆ, Gordana BRANKOVIĆ, Vera Đekić
NUTRITIONAL PROPERTIES OF MACEDONIAN LANDRACES OF small grain cereals AS a SOURCE OF NEW GENETIC VARIABILITY [Abstract] [Full text]

Ksenia A. KULIKOVA, Yusupjan A.YULDASHBAEV, Milan P.PETROVIC, Violeta CARO PETROVIC, Dragana RUZIC MUSLIC, Nevena MAKSIMOVIC, Dusica OSTOJIC ANDRIC

THE POLYMORPHISM OF CAST GENE IN RAMS POPULATIONS OF TUVAN BREED [Abstract] [Full text]

Irena RADINOVIĆ, Sanja VASILJEVIĆ, Miroslav ZORIĆ, Gordana BRANKOVIĆ, Tomislav ŽIVANOVIĆ, Slaven PRODANOVIĆ

VARIABILITY OF RED CLOVER GENOTYPES ON THE BASIS OF MORPHOLOGICAL MARKERS [Abstract] [Full text]

Masoud SHEIDAI, Fahimeh KOOHDAR, Zeinab Moradiyan POODE

MOLECULAR PHYLOGENY OF Lallemantia L. (LAMIACEAE): INCONGRUENCE BETWEEN PHYLOGENETIC TREES AND THE OCCURRENCE OF HGT [Abstract] [Full text]

Behrouz RAMAZANI-DEHNAVI, Abbas DOOSTI, Mohammad-Saeid JAMI

THE STUDY ON THE STIMULATION OF THE IMMUNE SYSTEM IN THE INFUSED BALB/c MICE BY PCDNA3.1(-)-FLAA RECOMBINANT VECTOR AGAINST Helicobacter pylori INFECTION USING MOLECULAR TECHNIQUES [Abstract] [Full text]

Ivana TOŠIĆ, Ljiljana BOŠKOVIĆ-RAKOČEVIĆ, Tihomir PREDIĆ, Novo PRŽULJ, Bojana SAVIĆ, Vojislav TRKULJA

ASSESSMENT OF DUTCH TOMATO HYBRIDS GROWN IN CONDITIONS OF WESTERN BOSNIA AND HERZEGOVINA [Abstract] [Full text]

Xiu-ping LI, Wen-dong MA, Wei CHANG, Chunbo LIANG, Hai-xin ZHAO,Jun-xiang GUO,Cheng-yan SONG, Guo-jun PAN

LINKAGE DISEQUILIBRIUM ANALYSIS OF RICE SHEATH BLIGHT RESISTANCE MARKERS OF RICE GROWN IN THE COLD REGION OF NORTHEAST CHINA [Abstract] [Full text]

Milan BIBERDŽIĆ, Jelena STOJILJKOVIĆ, Saša BARAĆ, Aleksandar DJIKIĆ, Danijela PRODANOVIĆ, Dragana LALEVIĆ

THE INFLUENCE OF HYBRIDS AND SOWING TERM ON YIELD AND DRY DOWN OF CORN GRAIN [Abstract] [Full text]

Mahdi BAYAT, Reza AMIRNIA, Hakan OZKAN, Aysun GEDIK, Duygu ATES, Mehdi RAHIM, Bahattin TANYOLAC

IDENTIFICATION OF MARKERS ASSOCIATED WITH TRAITS FOR USE IN MARKER-ASSISTED SELECTION IN SAFFRON [Abstract] [Full text]

Rameez RAJA, Naresh Bahadur SINGH,Sheeraz Saleem BHAT

ASSESSMENT OF GENETIC DIVERSITY AMONG HIGH YIELDING SELECTED SALIX CLONES, USING RAPD AND SSR MARKERS [Abstract] [Full text]

Tonmoy CHAKRABARTY, Umakanta SARKER, M. HASAN, and M. M. RAHMAN

VARIABILITY IN MINERAL COMPOSITIONS, YIELD AND YIELD CONTRIBUTING TRAITS OF STEM AMARANTH (Amaranthus lividus) [Abstract] [Full text]

Ida DJURDJEVIČ, Simona SUŠNIK BAJEC

LASER CAPTURE MICRODISSECTION OF PIGMENT CELLS FROM SALMONID FISH SKIN TO IDENTIFY DIFFERENTIALLY EXPRESSED GENES IN AN ENRICHED CELL POPULATION [Abstract] [Full text]

Jelena MESAROVIĆ, Jelena SRDIĆ, Snežana mladenović drinić, Vesna DRAGIČEVIĆ, Milena simić, Milan brankov, Dušanka milojković-opsenica

ANTIOXIDANT STATUS OF THE DIFFERENT SWEET MAIZE HYBRIDS UNDER HERBICIDE AND FOLIAR FERTILIZER APPLICATION [Abstract] [Full text]

Emre SEVİNDİKand Kübra YALÇIN

PHYLOGENETIC ANALYSIS OF SOME Citrus L. (RUTACEAE) TAXA IN TURKEY BASED ON CHLOROPLAST (cpDNA) trnL INTRON AND trnL-F DNA SEQUENCES [Abstract] [Full text]

Yalçın COŞKUN

EVALUATION OF DIFFERENT SIGMOIDAL GROWTH MODELS AND CLIMATE PARAMETERS FOR DRY MATTER ACCUMULATION OF OAT [Abstract] [Full text]

Bojana RISTANOVIĆ, Zoran. Ž. ILIĆ

EFFECT OF MOTHER’S AGE ON BODY WEIGHT OF LAMBS FROM BIRTH TO WEANING IN VARIOUS STRAINS OF DOMESTIC SHEEP PRAMENKA [Abstract] [Full text]

Dragana BRANKOVIĆ-RADOJČIĆ, Vojka BABIĆ, Zdenka GIREK, Tomislav ŽIVANOVIĆ, Aleksandar RADOJČIĆ, Milomir FILIPOVIĆ, Jelena SRDIĆ
EVALUATION OF MAIZE GRAIN YIELD AND YIELD STABILITY BY AMMI ANALYSIS [Abstract] [Full text]

Bojan MITROVIĆ, Dušan STANISAVLJEVIĆ, Filip FRANETA, Sanja MIKIĆ, Petar ČANAK, Bojana VUJOŠEVIĆ, Emilija NIKOLIĆ-ĐORIĆ

NON-PARAMETRIC APPROACH TO THE ANALYSIS OF PHENOTYPIC STABILITY OF TWO HALF-SIB MAIZE POPULATIONS [Abstract] [Full text]

Mueen Alam KHAN

ACHIEVEMENTS AND PROSPECTS OF MOLECULAR BREEDING FOR DROUGHT TOLERANCE IN SOYBEAN [Glycine max (L.) MERR.] [Abstract] [Full text]

Jan BIŃKOWSKI, Marek KMIEĆ, Jakub KUBIŚ

GENERAL CHARACTERISTICS OF THE MELANOGENESIS PROCESS WITH PARTICULAR EMPHASIS ON THE ROLE OF THE PAX3 GENE [Abstract] [Full text]

Svetlana PEROVIĆ, Gorana VEINOVIĆ,Jelena ANTIĆ STANKOVIĆ

A REVIEW ON ANTIBIOTIC RESISTANCE: ORIGIN AND MECHANISMS OF BACTERIAL RESISTANCE AS BIOLOGICAL PHENOMENON [Abstract] [Full text]

Snežana mladenović drinić, Kosana Konstantinov

50 years of journal Genetika (1968 – 2018) [Full text]

Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 755-770

UDC 575.633.15
https://doi.org/10.2298/GENSR1803755B
Orginal scientific paper

Assessment stability of maize lines yield by GGE-BIPLOT analysis

Dragan BOŽOVIĆ^1*, Tomislav ŽIVANOVIĆ^1*, Vera POPOVIĆ^2*, Mladen TATIĆ²,

Zagorka GOSPAVIĆ³, Zoran Miloradović⁴, Goran STANKOVIĆ⁵, Milorad ĐOKIĆ⁴

¹University of Belgrade, Faculty of Agriculture, Zemun–Belgrade, Serbia;

²Institute of Field and Vegetable Crops, Novi Sad, Serbia;

³University of Belgrade, Faculty of Civil Engineering, Belgrade, Serbia;

⁴University of Educons, Faculty of Ecological Agriculture, Sr. Kamenica, Serbia.

⁵Maize Research Institute Zemun Polje, Belgrade, Serbia

Abstract

Božović D., T. Živanović, V. Popović, M. Tatić, Z. Gospavić, Z. Miloradović, G. Stanković, M. Đokić (2018): Assessment stability of maize lines yield by GGE-biplot analysis.- Genetika, Vol 50, No.3, 755-770.

Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (G×L×Y×T) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010–2011) at the four treatments and two locations: Pančevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and G×L, G×T, Y×L, Y×T, L×T, G×Y×T, G×L×T, Y×L×T, G×Y×L×T interaction, on maize yield were significant (p<0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of G×L and G×T shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction G×L and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction G×L. The IPCA1 share in the G×T interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.

Key worlds: Zea mays L., lines, AMMI analysis, G×Y×L×T interaction, yield

Corresponding author: Dragan Božović, University of Belgrade, Faculty of Agriculture, Nemanjina 6, Zemun–Belgrade, Serbia; and Vera Popović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, E-mail: bravera@eunet.rs; stragrospancevo@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp.771-790

Serbia

UDC 575.
https://doi.org/10.2298/GENSR1803771M

Original scientific paper

ANALYSES OF GENETIC DIVERSITY OF BADIS BADIS (HAMILTON-BUCHANAN 1822) FROM THREE RIVERINE SYSTEMS IN SUB-HIMALAYAN BIODIVERSITY HOTSPOT OF WEST BENGAL, INDIA USING RAPD AND ISSR FINGERPRINTING

Tanmay MUKHOPADHYAY, Soumen BHATTACHARJEE*

Cell and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, India

Abstract

Mukhopadhyay T., S. Bhattacharjee (2018): Analyses of genetic diversity of Badis badis (Hamilton-Buchanan 1822) from three riverine systems in Sub-himalayan biodiversity hotspot of West Bengal, India using RAPD and ISSR fingerprinting.- Genetika, Vol 50, No.3, 771-790.

Badis badis is a threatened freshwater fish in the Indian scenario, and the population genetic architecture of this fish is largely unexplored in the eastern sub-Himalayan biodiversity hotspot of West Bengal, India, also known as the Terai and the Dooars. Total seventeen populations from three major river systems viz. Mahananda (Terai), Teesta and Jaldhaka (Dooars) have been studied through RAPD and ISSR fingerprinting. The polymorphism, genetic diversity and Shannon’s Information index were calculated for each population. The highest values for Nei’s genetic diversity (0.1436± 0.1963 and 0.1409 ±0.1954 after RAPD and ISSR analyses) and Shannon’s information index (0.2150±0.2794 and 0.2109 ±0.2785 after RAPD and ISSR analyses) were found in Jaldhaka river system. The UPGMA dendrogram revealed that the Mahananda and Teesta populations form a single group and the Jaldhaka population forms a separate group. Comparing with other related studies the genetic diversity Badis badis was found to be low in the three main riverine systems of the Terai and Dooars region of West Bengal, although the Jaldhaka population showed a comparatively higher level of genetic diversity. Therefore, Jaldhaka population should be managed and, conserved to preserve the available gene pool of this threatened species in this region. Low levels of genetic diversity were found in the present study among the seventeen populations validating the recent assignment of its threatened status.

Key words: Badis badis, RAPD, ISSR, genetic diversity, sub-Himalayan hotspot region.

Corresponding author: Soumen Bhattacharjee, e-mail: soumenb123@rediffmail.com / sbhnbu@gmail.com, Phone: +91-9474674013 (Cell)/+91-353-2776353 (O)

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp.791-802

Serbia

UDC 575.633.11
https://doi.org/10.2298/GENSR1803791G
Original scientific paper

ASSESSMENT OF GENETIC RELATIONSHIPS AMONG COMMON BUCKWHEAT (Fagopyrum esculentum MOENCH) VARIETIES FROM WESTERN BALKANS USING MORPHOLOGICAL AND SSR MOLECULAR MARKERS

Jasmin GRAHIĆ^1*, Mirha ĐIKIĆ¹, Drena GADŽO¹, Silvio ŠIMON², Mirsad KURTOVIĆ¹,

Ivan PEJIĆ³, Fuad GAŠI¹

¹Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

²Institute of Viticulture and Enology, Croatian Center for Agriculture, Food and Rural Affairs, Zagreb, Croatia

³Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia

Abstract

Grahić J., M.Đikić, D. Gadžo, S. Šimon, M. Kurtović, I. Pejić, F. Gaši (2018): Assessment of genetic relationships among common buckwheat (Fagopyrum esculentum Moench) varieties from Western Balkans using morphological and SSR molecular markers.- Genetika, Vol 50, No., 791-802.

In order to assess the genetic relationships and diversity among common buckwheat from Western Balkans, three varieties produced under the names ‘Čebelica’, ‘Darja’ and ‘Goluba’ were evaluated with a set of 10 SSR and 32 morphological markers. Eight of ten primer pairs used managed to amplify SSR alleles, in average 7 alleles per locus. Analysis of molecular variance (AMOVA) revealed that only 2.6% of the total diversity was attributed to the differences among ‘Darja’ and ‘Goluba’. The largest percentage of variance between varieties was detected among ‘Goluba’ and ‘Čebelica’ (f_CT = 0.136; p < 0.001). Factorial correspondence analysis also revealed a clear differentiation between these two varieties. Results of hierarchical clustering based on morphological data of the three analyzed common buckwheat varieties were not in a complete accordance with the results obtained through genetic analyses, as it displayed a much larger difference between ‘Darja’ and ‘Čebelica’, than between ‘Goluba’ and ‘Čebelica’. However, the differentiation among the analyzed varieties based on SSRs and Euclidean distances, calculated using morphological data, was the same. Namely, both approaches identified ‘Čebelica’ as the most divergent material among the analyzed varieties. The results of the genetic characterization indicate that the purity of varieties of cross pollinated species produced in Western Balkans is questionable due to use of seed material of unverified origin, as well as the general use of farm saved seeds.

Key words: common buckwheat, SSR, morphological markers, factorial correspondence analysis, hierarchical clustering.

Corresponding author: Jasmin Grahić, PhD; Faculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina. Telephone number: +387 33 225 727; Fax number: +387 33 667 429; E-mail: grahic.jasmin@live.com.

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Published in „GENETIKA“ Vol. 50, No3(2018), pp. 803-816

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803803O
Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY OF Smallanthus sonchifolius (Poepp. & Endl.) H. ROBINSON LANDRACES BY USING AFLP MARKERS

Jaroslava OVESNÁ¹, Daniela RUSSO^2*, Domenico FRESCURA², Eloy Fernández CUSIMAMANI^3*, Eva SVOBODOVÁ³, Luigi MILELLA²

¹Department of Molecular Biology, Crop Research Institute, Prague, Czech Republic

²University of Basilicata, Department of Science, Potenza, Italy

³Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Prague 6, Suchdol, Czech Republic

Abstract

Ovesná J., D. Russo, D. Frescura, E. F. Cusimamani, E. Svobodová, L. Milella (2018): Assessment of genetic diversity of Smallanthus sonchifolius (Poepp. & Endl.) h. Robinson landraces by using AFLP markers.- Genetika, Vol 50, No.3, 803-816.

AFLP (Amplified Fragment Length Polymorphism) analysis was carried out on Smallanthus sonchifolius to increase the knowledge on its genetic diversity. It is an ethnomedical and edible plant native of Peru and cultivated in many other countries. Thirteen landraces were analyzed by selected AFLP primer combinations generating a number of 185 fragments, of which 180 were polymorphic (97.00% of polymorphism). The mean value of fragments per primer combination was 37, but MseI (M)-CAG/EcoRI (E)-ACT primer combination reported the highest number with 63 amplicons, instead only 27 were revealed by M-CAG/E-ACC. The marker attributes such as resolving power (RP), marker index (MI) and polymorphism information content (PIC) were determined. RP values varied from 11.54 (M-CAG/E-ACC) to 27.54 (M-CAG/E-ACT), PIC ranged from 0.25 (M-CAG/E-AGC) to 0.28 (M-CAG/E-ACA), whereas MI values were found to be in the range from 6.18 (M-CAG/E-ACC) to 15.95 (M-CAG/E-ACT). Cluster analysis and PCA were evaluated for determining relationships among yacon landraces. We concluded that AFLP markers showed a highest efficiency in estimating genetic diversity in yacon despite to previous paper in which 3 times lower samples have been analyzed.

Keywords: Ampliﬁed Fragment Length Polymorphism; DNA; genetic variation; molecular markers; Yacon.

Corresponding author: Daniela Russo, University of Basilicata, Department of Science, V.le dell’Ateneo Lucano, 85100 Potenza, Italy, Daniela.russo@unibas.it; tel.+39 0971 205525 fax +39 0971 205501

Eloy Fernández Cusimamani, Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Kamýcká 129, 165 21 Prague 6, Suchdol, Czech Republic, eloy@ftz.czu.cz tel/fax +420 22438 2183.

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 817-828

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1803817M

Original scientific paper

VARIABILITY, CORRELATION, PATH ANALYSIS AND STEPWISE REGRESSION FOR YIELD COMPONENTS OF DIFFERENT WHEAT GENOTYPES

Mirela MATKOVIC STOJSIN^1*, Veselinka ZECEVIC¹, Sofija PETROVIC²,

Miodrag DIMITRIJEVIC², Danica MICANOVIC³, Borislav BANJAC², Desimir KNEZEVIC⁴

¹Megatrend University, Faculty of Biofarming, Backa Topola, Serbia,

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia,

³Chamber of Commerce and Industry of Serbia, Belgrade, Serbia

⁴University of Pristina, Faculty of Agriculture, Kosovska Mitrovica, Lešak,

Kosovo and Metohia, Serbia.

Abstract

Matković Stojšin, M., V. Zečević, S. Petrović, M. Dimitrijević, D. Mićanović, B. Banjac, D. Knežević(2018): Variability, correlation, path analysis and stepwise regression for yield components of different wheat genotypes.- Genetika, Vol 50, No.3, 817-828.

In order to evaluate the variability and relationship between different wheat yield components, a randomized complete block design experiment with ten genotypes of wheat had been carried out during three growing seasons (2010-2012). The number of spikelet per spike and grain weight per spike had low genotypic and phenotypic variability, while plant height had the highest one. High heritability was observed for plant height (h²=93.1%), spike length (h²=92.3%) and spike density (h²=92.9%). The low heritability was found for grain weight per spike (h²=35.6%). Grain weight per spike was in significant positive genotypic and phenotypic correlation with all the traits (plant height, spike height, number of spikelet per spike, number of grain per spike and spike weight) except spike density. The spike weight had the highest phenotypic (r_p=0.988), while number of spikelet per spike had the highest genotypic correlation with grain weight per spike (r_g=0.981). Path coefficient analysis revealed that all the traits had highly significant direct effect on grain weight per spike, except spike length. The stepwise regression revealed that 87.1% of the grain weight per spike variation was explained by model which excludes spike length. Spike weight and plant height had the highest shared and unique contribution to grain weight per spike.

Keywords: yield components, correlation, heritability, variability, wheat.

Corresponding author: Mirela Matković Stojšin, Megatrend University, Faculty of Biofarming, Marsala Tita 39, Backa Topola, Serbia, Phone +381 24 712 209, e-mail: mirelam89@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 829-836

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803829A

Original scientific paper

RESULTS OF ENZYME REPLACEMENT THERAPY IN BULGARIAN PATIENTS WITH A SEVERE FORM OF HUNTER SYNDROME: A 42-MONTH FOLLOW-UP

Daniela AVDJIEVA-TZAVELLA¹, Hadil KATHOM¹, Ivanka SINIGERSKA², Zlatina TASEVA³,

Radka TINCHEVA¹

¹Department of Clinical Genetics, University Pediatric Hospital, Medical University, Sofia, Bulgaria

²National Genetic Laboratory, Sbalag, Majcin Dom, Medical University, Sofia, Bulgaria

³Kinesiotherapist, University Pediatric Hospital, Medical University, Sofia, Bulgaria

Abstract

Avdjieva-TzavellaD., H. Kathom, I. Sinigerska, Z. Taseva, R. Tincheva (2018): Results of enzyme replacement therapy in Bulgarian patients with a severe form of Hunter syndrome: a 42-month follow-up.- Genetika, Vol 50, No.3, 829-836.

Hunter syndrome (Mucopolysaccharidosis type II, MPS II) is a rare X-linked disease caused by a deficiency of the enzyme iduronate-2-sulphatase (IDS), which results in the lysosomal accumulation of the undegraded glycosaminoglycans (GAGs) dermatan and heparan sulfate in various tissues and organs. Enzyme replacement therapy (ERT) with recombinant iduronate-2-sulphatase is the first disease-specific treatment for Hunter syndrome. Clinical trial data for the use of idursulfase to treat severe Hunter patients are limited and controversial. Our study analyzes therapeutic responses after ERT over 42 months of five Hunter patients and further expanding the knowledge of benefits and disadvantages of such therapy. Five boys with the severe form of MPS II (age range, 5−17 years) were treated with idursulfase for a minimum period of 8 months to a maximum period of 42 months. ERT with idursulfase in patients with the severe form of MPS II was associated with improvements in urinary GAG excretion and spleen size, stabilization of cardiac disease, and not effective on joint contractures, and on liver volume. MPS II is a progressive disease and response to ERT is influenced by the severity of the phenotype at treatment initiation.

Keywords: Enzyme replacement therapy, hunter syndrome, idursulfase, mucopolysaccharidosis II, severe form.

Corresponding author: Daniela Avdjieva-Tzavella, University Pediatric Hospital, Department of Clinical Genetics, 11 Acad. Ivan Geshov str., Sofia 1606, Bulgaria; Tel: +359888124230; e-mail address: davdjieva@yahoo.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp.837-846

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803837T

Original scientific paper

FREQUENCY OF GJB2 MUTATIONS IN FAMILIES WITH AUTOSOMAL RECESSIVE NON-SYNDROMIC HEARING LOSS IN KHUZESTAN PROVINCE

Parisa TAHMASEBI^1,2, Morteza Hashemzadeh CHALESHTORI³, Fatemeh ABDOLLAHNEJAD⁴, Zahra ALAVI⁴, Ladan SADEGHIAN³, Farah TALEBI⁵, Javad MOHAMMADI-ASL⁶, Nader SAKI⁷, Seyed Reza Kazemi NEZHAD¹, Mohammad Amin TABATABAIEFAR^8,9*

¹Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

²Department of Biology, Faculty of Science, Ilam University, Ilam, Iran

³Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

⁴Department of Molecular Genetics, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

⁵Genetic Counseling Center, Welfare Organization of Ahvaz, Ahvaz, Iran

⁶Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

⁷Department of Otolaryngology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

⁸Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

⁹Genetics department, Erythron Pathobiology and Genetics lab, Isfahan, Iran

Abstract

Tahmasebi P., M. H. Chaleshtori, F. Abdollahnejad, Z. Alavi, L. Sadeghian, F.Alebi, J.ad Mohammadi-Asl, N. Saki, S. R. K. Nezhad, M. A. Tabatabaiefar (2018): Frequency of gjb2 mutations in families with autosomal recessive non-syndromic hearing loss in Khuzestan province.- Genetika, Vol 50, No.3, 837-846.

Hearing loss is caused both by genetic and environmental factors. In this sense, more than half of the cases are genetic. Hereditary hearing loss is divided into syndromic and non-syndromic cases. Main pattern of inheritance (80%) in non-syndromic cases is autosomal recessive, which is known as autosomal recessive non-syndromic hearing loss (ARNSHL). Although the disease is very genetically heterogeneous, the GJB2 gene has highest effect. The aim of the present research is to determine the role of GJB2 mutations and DFNB1 locus in a group of families with ARNSHL in Khuzestan province of Iran. This study was conducted on 50 large families with ARNSHL (with the priority of 4 patients) in Khuzestan province, southwest Iran. First, in order to study GJB2 gene mutations, direct sequencing was conducted in all families. Besides, in the families with negative or heterozygous mutations in GJB2, linkage analysis was done using STR (Short Tandem Repeat) markers related to DFNB1 locus. Genotypes, related to each family, were determined using PCR-PAGE method. From 50 families with ARNSHL, after direct sequencing, six families (12%) showed homozygote mutations in GJB2 gene. Accordingly, it was observed that c.35delG was the most frequent mutation (50%) in the population. Moreover, c.358-360delGAG, c.506G>A, c.71G>A mutations were identified in 3 different families. In addition, no linkage was observed in DFNB1 locus for the remaining families. Low frequency (12%) of GJB2 mutations in our study suggests that other loci and genes should be addressed to elucidate the etiology of ARNSHL in this province.

Keywords: RNSHL, GJB2, DFNB1 locus, Iran.

Corresponding author: Mohammad Amin Tabatabaiefar,Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Hezarjarib St., Isfahan 81746-73461, Iran, Tel: (098) (0)31 3792 2487, E-mail: tabatabaiefar@med.mui.ac.ir

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp.847-854

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803847C

Original scientific paper

ASSOCIATION BETWEEN IL-4 GENE POLYMORPHISMS AND CHRONIC OBSTRUCTIVE PULMONARY DISEASE OF PATIENTS IN HEBEI PROVINCE, CHINA

Yumin CHI, Qingguo DI, Guangchao HAN, Min LI, Baohua SUN*

Respiratory Department, Cangzhou Central Hospital, Cangzhou 061001, Hebei Province,

P.R. China

Abstract

Chi Y., Q. Di, G. Han, M. Li, B. Sun (2018): Association between Il-4 gene polymorphisms and chronic obstructive pulmonary disease of patients in Hebei province, China.- Genetika, Vol 50, No.3, 847-854.

We aimed to study the association between IL-4 gene polymorphisms and chronic obstructive pulmonary disease (COPD) of patients in Hebei Province. The blood samples of 62 COPD patients and 301 healthy subjects were collected. IL-4 gene polymorphisms were detected by PCR with sequence-specific primer typing. There were significant differences in the alleles of IL-4-33, IL-4-590 and IL-4-1098 between COPD patients and healthy subjects (P<0.05). IL-4-590/C (P<0.001, OR = 4.619, CI = 1.640-7.985) had the highest susceptibility, suggesting that people with this allele were 3.6 times more prone to COPD than those with IL-4-33-590/T allele. COPD of susceptible patients was positively associated with IL-4-33/C:C (P<0.001, OR = 5.3, CI = 0.939-7.714), IL-4-590/C:C (P<0.001, OR = 29.5, CI = 11.138-57.647) and IL-4-1098/T:T (P<0.001, OR = 3.3, CI = 1.016-7.862). According to the level of protectiveness, COPD of patients (protective) was negatively associated with IL-4-33/C:T (P = 0.009, OR = 0.109, CI = 0.015-0.809), IL-4-590/C:T (P = 0.009, OR = 0.397, CI = 0.195-0.810), IL-4-1098/G:G (P<0.001, OR = 0.133, CI = 0.054-0.331) and IL-4-1098/G:T (P<0.001, OR = 0.209, CI = 0.104-0.527). IL-4/TCT (P<0.001, OR = 35.500, CI = 11.30-111.515) was found in susceptible patients. Negative association was found between COPD and haplotypes: IL-4/TTC (P<0.001, OR = 0.061, CI=0.008-0.443) and IL-4/GCC (P<0.001, OR = 0.193, CI = 0.076-0.488). IL-4/GCT, IL-4/GTC and IL-4/GTT were only found in healthy subjects. IL-4 gene polymorphisms (loci -1098, -590 and -33) were closely associated with COPD in Hebei Province.

Keywords: association, IL-4, genetic polymorphism, chronic obstructive pulmonary disease

Corresponding author: Baohua Sun, Respiratory Department, Cangzhou Central Hospital, Cangzhou 061001, Hebei Province, P.R. China, E-mail: sunbhcch@hotmail.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 855-862

Serbia

UDC 575.630.6

https://doi.org/10.2298/GENSR1803855S

Original scientific paper

GENETIC TREND OF FUNCTIONAL PRODUCTIVE LIFE IN THE POPULATION

OF BLACK AND WHITE CATTLE IN SERBIA

Dragan STANOJEVIĆ¹*, Radica DJEDOVIĆ¹, Vladan BOGDANOVIĆ¹, Nikola RAGUŽ², Denis KUČEVIĆ³, Mladen POPOVAC¹, Petar STOJIĆ⁴, Ljiljana SAMOLOVAC⁵

¹Department of Animal Science, Faculty of Agriculture, University of Belgrade, Serbia

²Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, Croatia

³Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Serbia

⁴Institute PKB Agroekonomik, Serbia

⁵PKB Corporation Belgrade, Serbia

Abstract

Stanojević D., R. Djedović, V. Bogdanović, N. Raguž, D. Kučević, M. Popovac, P. Stojić, Lj. Samolovac (2018): Genetic trend of functional productive life in the population of black and white cattle in Serbia.- Genetika, Vol 50, No.3, 855-862.

This research was conducted with the aim оf estimating genetic trend for a functional length of productive life (FLPL) in the population of Black and White cattle in Serbia. Research and bulls genetic evaluation for functional longevity were performed on a set of data provided by Agricultural Corporation Belgrade AD (PKB) which contained data on longevity and origin of 22109 cows out of which 26% records were right-censored. Functional length of productive life (FPLP) represents a time period from the first calving to culling or censoring corrected for milk production. Breeding values were estimated using Weibull method of proportional risks within survival analysis a genetic trend for FLPL was calculated using of a regression analysis. The cows included in the analysis were on average first calved in the age of 809 days and had an average share of 81.9% genes of Holstein Friesian breed. An average length of productive life was 1267 days (41.6 months). Distribution of bull standardised breeding values did not statistically significantly differ from normal distribution. An average standardised breeding value was 99. A slightly positive genetic trend was determined, that is, the length of functional productive life was by selection increased by 0.021 day at an annual level while a reliability of estimated breeding values showed a negative trend.

Key words: functional longevity, genetic trend, black and white cattle

Corresponding author: Dragan Stanojević, Department of Animal Science, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Zemun, Serbia, phone: +38111 4413555, E-mail: stanojevic@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 863-883

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1803863J

Original scientific paper

NUTRITIONAL PROPERTIES OF MACEDONIAN LANDRACES OF small grain cereals AS a SOURCE OF NEW GENETIC VARIABILITY

Suzana JORDANOVSKA¹, Zoran JOVOVIĆ¹, Željko DOLIJANOVIĆ³, Vesna DRAGIČEVIĆ⁴, Gordana BRANKOVIĆ⁵, Vera Đekić⁶

¹University Ss. “Cyrilus and Methodius”, Institute of Agriculture, Skopje, Macedonia
²University of Montenegro, Biotechnical Faculty Podgorica, Podgorica, Montenegro

³University of Belgrade, Faculty of Agriculture, Department of Agrotechnic and Agroecology, Belgrade, Serbia

⁴Maize Research Institute “Zemun Polje”, Belgrade, Serbia

⁵University of Belgrade, Faculty of Agriculture, Department of Genetics, Plant Breeding and Seed Sceince, Belgrade, Serbia

⁶Center for small grains, Kragujevac, Serbia

Abstract

Jordanovska S., Z. Jovović, Ž. Dolijanović, V. Dragičević, G. Branković, V. Đekić (2018): Nutritional properties of macedonian landraces of small grain cereals as a source of new genetic variability.- Genetika, Vol 50, No.3, 863 - 883.

Taking into account the better agro-ecological adaptations developed over time to climate changed conditions, cereal local populations (landraces) represent a valuable plant genetic resources with their perspective reflected in the creation of better quality commercial cereal genotypes. The objectives of this research were to explore: i) the genetic variability of nutritional properties of Macedonian landraces of small grain cereals-wheat, barley, oat, and rye; ii) associations among nutritional properties; iii) strength and weakness of landraces based on nutritional properties profiles. Collecting missions were carried out in 2013 year in different locations of rural areas at the territory of Republic of Macedonia. Ten sub-samples of 100 g seeds were extracted from each of regenerated landrace in order to obtain a well-balanced analytical sample. All samples were analysed for moisture content - MOI (%), protein content - PC (%), fat content - FC (%), crude fibre content - CF (%), wet gluten content - WG (%), and dry gluten content - DG (%). In regard to assessed nutritional properties the most perspective landraces proved to be: Okalesta bela (CF of = 2.62%) of bread wheat; Zimski (WG of = 9.24%), Dabilski nizok (DG of = 4.2%) and Ednoreden (CF of = 5.18%) of barley; Šopski (PC of = 14.62%), Gabarski (FC of = 6.46%) and Sekulički (CF of = 9.89%) of oat; Čalakliski (PC of = 14.43%, CF of = 8.16%), Koselski (FC of = 4.19%), and Gabarski (DG of = 3.14%) of rye. The positive associations among nutritional properties of Macedonian landraces of small grain cereals were: all examined nutritional properties except PC and CF in bread wheat landraces; PC, WG, DG, MOI as one cluster, and FC and CF as another cluster in barley landraces; all examined nutritional properties except MOI and CF in oat landraces; PC, CF, FC as one cluster and DG and MOI as another cluster in rye landraces. The Macedonian landraces of small grain cereals proved to be new sources of genetic variability of nutritional properties which can be used in breeding, because they outperformed commercial check cultivar landraces with statistical significance (P < 0.05) for: MOI (4 landraces) and CF (4 landraces) for bread wheat; WG (Zimski), DG (3 landraces), CF (7 landraces) for barley; CF (5 landraces) for oat; DG (1 landrace), MOI (4 landraces), FC (4 landraces in rye).

Key words: bread wheat, barley, oat, rye, nutritional quality

Corresponding author: Zoran Jovović, University of Montenegro, Biotechnical Faculty Podgorica, Mihaila Lalića 1, 81000 Podgorica, Montenegro, email:zoran.jovovic.btf@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 885-893

Serbia

UDC 575.630.6

https://doi.org/10.2298/GENSR1803885K

Original scientific paper

THE POLYMORPHISM OF CAST GENE IN RAMS POPULATIONS

OF TUVAN BREED

Ksenia A. KULIKOVA¹, Yusupjan A.YULDASHBAEV¹, Milan P.PETROVIC²,

Violeta CARO PETROVIC², Dragana RUZIC MUSLIC², Nevena MAKSIMOVIC²,

Dusica OSTOJIC ANDRIC²

¹Federal State Budgetary Educational Institution of Higher Education “Russian Timiryazev State Agrarian University”, Faculty of Animal Science and Biology, Moscow, Russian Federation

² Institute for Animal Husbandry, Belgrade, Republic of Serbia

Abstract

Kulikova K.A., Y.A. Yuldashbaev, M. P. Petrovic, V. Caro Petrovic, D. Ruzic Muslic,
N. Maksimovic, D. Ostojic Andric (2018): The polymorphism of CAST gene in rams populations of Tuvan breed.- Genetika, Vol. 50, No.3, 885-893.

The Tuva Republic is one of the main sheep breeding centers in the Russian Federation. The Tuvan short-fat-tailed sheep is a unique local breed of the Tuva Republic. The studied breed has sheep of steppe type and mountain type. Each type of sheep was having formed according to relief of the ground and environment features. The breed originated in different parts from steppe and mountains. This study aim was to show the genetic polymorphism of calpastatin gene (CAST) in ram populations of two main breeding centers for tuvan short-fat-tailed sheep. Genomic DNA was isolated from 51 animals from Municipal unitary enterprise "Despen" (steppe type of sheep), and 100 animals from State unitary enterprise "Malchyn" (mountain type of sheep). Genomic DNA was extracted using the commercial kits. The detection was done using the restriction fragment length polymorphism of the polymerase chain reaction products (PCR-RFLPs). We used a pair of primers: F: 5’-TGGGGCC CAATGACGCCATCGATG-3’ and R: 5’-GGTGGAGCAGCACTTCTGATCACC-3’ to get 622 b.p. fragment of CAST gene. The PCR products are having digested with MspI restriction enzyme. Calpastatin locus digested with MspI has shown three genotypes: MM, MN and NN. The frequency of MM, MN and NN genotypes were 80.39%, 17.65% and 1.96% in steppe type of breed and 77.00%, 22.00% and 1.00% in the mountain type of breed. The highest allelic frequency was for allele M (0.89 in MUE "Despen" rams and 0.88 in SUE "Malchyn" rams). Allelic frequency of allele N had lower values of 0.11 in steppe type and 0.12 in mountain type.

Keywords: Sheep, Calpastatin gene, CAST, PCR-RFLP, genetic polymorphism

Corresponding author: Ksenia A. Kulikova, Federal State Budgetary Educational Institution
of Higher Education “Russian Timiryazev State Agrarian University”, Faculty of Animal Science and Biology, 49, Timiryazevskaya st., Building 5, Moscow, 127550, Russian Federation.
Tel: +(499) 976-0236, Fax: +(499) 976-1447, e-mail: dvulunie@yandex.ru

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Published in „GENETIKA“ Vol. 50, No.3(2018), pp. 895-906

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803895R

Original scientific paper

VARIABILITY OF RED CLOVER GENOTYPES ON THE BASIS OF MORPHOLOGICAL MARKERS

Irena RADINOVIĆ^1*, Sanja VASILJEVIĆ², Miroslav ZORIĆ², Gordana BRANKOVIĆ¹, Tomislav ŽIVANOVIĆ¹, Slaven PRODANOVIĆ¹

¹ Department of Genetics and Plant Breeding, Faculty of Agriculture, University of Belgrade, Zemun-Belgrade, Serbia

²Forage Crops Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Radinović I., S. Vasiljević, M. Zorić, G. Branković, T. Živanović, S.Prodanović (2018): Variability of red clover genotypes on the basis of morphological markers.- Genetika, Vol 50, No.3, 895- 906.

Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic distances represented by the simple matching coefficient of similarity ranging from 0.00 to 1.00. Observed results achieved by these two analyses were compared and although both of analyses were successful in grouping and discrimination of red clover genotypes with some similarities and differences, priority in future breeding programs was given to the HOMALS.

Keywords: red clover, variability, morphological markers, homogeneity analysis, cluster analysis

Corresponding author: Irena Radinović, Department of Genetics and Plant Breeding, Faculty of Agriculture, University of Belgrade, Zemun-Belgrade, Serbia, phone: +381 114413280,Fax: +381 11441352, email:calic@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 907-918

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803907S

Original scientific paper

MOLECULAR PHYLOGENY OF Lallemantia L. (LAMIACEAE): INCONGRUENCE BETWEEN PHYLOGENETIC TREES AND THE OCCURRENCE OF HGT

Masoud SHEIDAI^*, Fahimeh KOOHDAR, Zeinab Moradiyan POODE

Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran.

Abstract

Sheidai M., F. Koohdar, Z. M. Poode (2018): Molecular phylogeny of Lallemantia L. (Lamiaceae): incongruence between phylogenetic trees and the occurrence of HGT.- Genetika, Vol 50, No.3, 907-918.

The genus Lallemantia Fisch. & C. A. Mey. (Family Lamiaceae) is a small genus with only five species that are herbaceous annual or biennial plants with food and medicinal value. This genus is of Caucasian origin and contains 5 species in Iran. The aims of the present study were: 1- to examine the occurrence of phylogenetic conflict between nuclear (ribosome ITS), nuclear repetitive sequences (ISSRs) and plastid (rps16 intron, cp) sequences in the genus Lallemantia (Lamiaceae), 2- to investigate the occurrence of inter-specific hybridization in this genus, and 3- to compare the time of divergence of the species from the basal line by ITS and cp-DNA molecular data. This is the first analysis on these evolutionary aspects of the genus Lallemantia. We provided the first molecular evidence for the occurrence of inter-specific hybridization in the genus Lallemantia and illustrated that phylogenetic signals in cp-DNA and ITS sequences differ significantly.

Keyword: Lallemanti, hybridization, cpDNA, ITS

Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran. e-mail:msheidai@yahoo.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 919-932

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803919R

Original scientific paper

THE STUDY ON THE STIMULATION OF THE IMMUNE SYSTEM IN THE INFUSED BALB/c MICE BY PCDNA3.1(-)-FLAA RECOMBINANT VECTOR AGAINST Helicobacter pylori INFECTION USING MOLECULAR TECHNIQUES

Behrouz RAMAZANI-DEHNAVI¹, Abbas DOOSTI^2*, Mohammad-Saeid JAMI^1,3

¹Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

²Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

³Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Ramazani-Dehnavi B., A. Doosti, M.S. Jami (2018): The study on the stimulation of the immune system in the infused BALB/C mice by pCDNA3.1(-)-flaA recombinant vector against Helicobacter pylori infection using molecular techniques.- Genetika, Vol 50, No.3, 919 - 932.

Helicobacter pylori, a Gram-negative flagellated microaerophilic bacterium, is associated with inflammation of the stomach, duodenal and gastric ulcer disease, and gastric cancer. This bacterium infects almost half of the world's population stomachs. One of this pathogen’s immunogenic genes is the flaA gene that can stimulate the host immune system. In our previous study, the immune response in pCDNA3 1(-)-flaA infused BALB/c mice against H. pylori infection was investigated using quantitative real-time PCR (q-RT-PCR). After preparation of pCDNA3 1(-)-flaA recombinant plasmid at large-scale, the mice were infused in the hip muscle by a recombinant vector with or without chitosan nanoparticles. The pcDNA3 1(-) was used as the negative control. The blood and tissue specimens of each mouse were collected at different times. The expression levels of cytokine genes (including IL-2, IFNγ, IL4) and the internal control gene were evaluated in peripheral blood cells using a q-RT-PCR method. Also, the flaA gene expression in mice muscle was measured at 15, 30, and 45 days after the last injection. In infused mice by pcDNA3 1(-)-flaA, the IL-2 and IFNγ genes were increased statistically (p <0.001) and IL4 was significantly decreased (p <0.001). Moreover, the expression of flaA gene in mice muscles was decreased by passing the time. Furthermore, the infused mice by pcDNA3 1(-)-flaA + nanoparticles showed a better immune response because of alteration in IL4 expression. Our findings in infused mice by pcDNA3 1(-)-flaA suggested that the expression level of IL-2 and IFNγ were increased compared to the IL-4 via simulation of Th1. Also, the expression of the flaA gene in tissue samples was decreased 45 days after the last injection. Therefore, it can be concluded that the pcDNA3.1(-)-flaA recombinant vector together with chitosan nanoparticles has the ability to stimulate the immune system and it can be investigated as a cost-effective method to control the H. pylori disease in a human in further studies.

Keywords: Helicobacter pylori, flaA gene, immune stimulation, cytokines gene expression

Corresponding author: Abbas Doosti, Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, Postal box: 166; Tell: +98-38-33361001. Fax: +98-38-33361001; E-mail: biologyshki@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 933-942

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803933T

Original scientific paper

ASSESSMENT OF DUTCH TOMATO HYBRIDS GROWN IN CONDITIONS

OF WESTERN BOSNIA AND HERZEGOVINA

Ivana TOŠIĆ¹*, Ljiljana BOŠKOVIĆ-RAKOČEVIĆ², Tihomir PREDIĆ¹, Novo PRŽULJ³, Bojana SAVIĆ¹, Vojislav TRKULJA¹

¹Agricultural Institute of Republic of Srpska, Banja Luka, Republic of Srpska, Bosnia and Herzegovina

²University of Kragujevac, Faculty of Agronomy Čačak, Čačak, Serbia

³ University of Banja Luka, Faculty of Agronomy, Banja Luka, Republic of Srpska, Bosnia and Herzegovina

Abstract

Tošić I., Lj. Bošković-Rakočević, T. Predić, N. Pržulj, B. Savić, V. Trkulja (2018): Assessment of Dutch tomato hybrids grown in conditions of Western Bosnia and Herzegovina.- Genetika, Vol 50, No.3, 933 - 942.

The comparative estimation was carried out with seven tomato hybrids (Lycopersicon esculentum Mill.) during the period 2011 to 2013 in greenhouse at Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia and Herzegovina. The aim of this study is to examine the dry matter content, lycopene in fruits and total yield of Dutch tomato hybrids in the conditions of western Bosnia and Herzegovina. The experiment was set up by a randomized block design in three repetitions. The dry matter content ranged from 5.60% to 8.14%. Hybrid and hybrid x year interaction had significant (p<0.05) influence on lycopene content and tomato yield. The factors that had a decisive influence on the content of dry matter, lycopene content in fruit and tomato yield were year, hybrid and cultivation technology.

Keywords: Lycopersicon esculentum Mill., hybrid, dry matter, lycopene, yield

Corresponding author: : Ivana Tošić, Agricultural Institute of Republic of Srpska, Banja Luka, Knjaza Miloša 17, 78000, Banja Luka, Republic of Srpska, Bosnia and Herzegovina, it.tosic@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 943-958

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1803943L

Original scientific paper

LINKAGE DISEQUILIBRIUM ANALYSIS OF RICE SHEATH BLIGHT RESISTANCE MARKERS OF RICE GROWN IN THE COLD REGION OF NORTHEAST CHINA

Xiu-ping¹LI^,2# , Wen-dong³MA^# , Wei CHANG ⁴, Chunbo LIANG ^1,5, Hai-xin ZHAO ^1,3,

Jun-xiang GUO ³,Cheng-yan SONG ³, Guo-jun PAN ^1,3*

¹Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,150086, Harbin, Heilongjiang;

²School of Life Sciences, Jiamusi University, 154007, Jiamusi Heilongjiang;

³Rice Research Institute, Heilongjiang Academy of Agricultural Sciences, 154026, Jiamusi Heilongjiang;

⁴College of Agricultural Engineering, Nanyang Normal University，473061, Nanyang, Henan

⁵Industrial crops institute, Heilongjiang Academy of Agricultural Sciences, 150086, Harbin, Heilongjiang

Abstract

Li X., W. Ma, W. Chang, C. Liang, H. Zhao,J. Guo,C. Song, G. Pan (2018): Linkage disequilibrium analysis of rice sheath blight resistance markers of rice grown in the cold region of Northeast China.- Genetika, Vol 50, No.3, 943-958.

A total of 192 native rice germplasm lines from the cold region of Northeast China were analyzed for rice sheath blight resistance at the late tillering stage using the toothpick embedding method. Disease rating, absolute disease lesion height, relative lesion length and lesion length served as indicators of sheath blight resistance. Germplasm resources were then subjected to population structure analysis and linkage disequilibrium analysis in order to identify disease resistance markers for use in marker-assisted breeding for sheath blight resistance. The germplasm resources were segregated into three main groups .Notably, a total of nine markers were significantly associated with rice sheath blight resistance; eight of these markers(OSR23, RM270, RM339, RM470, RM536, RM569,RM1022, RM1163) were associated with disease rating. The additional marker RM283 was associated with both relative lesion length and lesion length and made a high contribution to the rate of phenotypic variation. Ultimately, the sheath blight resistance markers described in this study could be useful for rice marker-assisted selection of sheath blight resistant cultivars breeding.

Keywords: Japonica rice (Oryza sativa L.), cold region, sheath blight, linkage disequilibrium analysis, marker-assisted selection (MAS).

Corresponding author: Pan Guo-jun, Rice Research Institute Heilongjiang Academy of Agricultural Sciences, 154026, Jiamusi Heilongjiang, sdsguojunpan@163.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp.959-970

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1803959B

Original scientific paper

THE INFLUENCE OF HYBRIDS AND SOWING TERM ON YIELD AND DRY DOWN OF CORN GRAIN

Milan BIBERDŽIĆ¹, Jelena STOJILJKOVIĆ², Saša BARAĆ¹, Aleksandar DJIKIĆ¹, Danijela PRODANOVIĆ¹, Dragana LALEVIĆ¹

¹University in Pristina, Faculty of Agriculture, Lešak, Serbia

²Agriculture Advising and Consulting Department, Leskovac, Srbija

Abstract

Biberdžić M, J. Stojiljković, S. Barać, A. Djikić, D. Prodanović, D. Lalević (2018): The influence of hybrids and sowing term on yield and dry down of corn grain.- Genetika, Vol 50, No.3, 959-970.

The dry down rate of corn grain during maturation is influenced by a large number of environmental factors and properties of hybrids, and has a major effect on corn, reaching its full maturity. This is especially important for the timely soil tillage and planting of the next culture. The aim of this research was to determine the yield and dry down rate from corn grains depending on the hybrid and the sowing term, in the agro ecological conditions of South Serbia (Leskovac). For this purpose, an experiment was made during 2016 and 2017 on the territory of the municipality of Leskovac. Soil type was alluvium. The trial was planned according to the schedule of divided land plots, set up in three repetitions. This included 6 hybrids of different FAO maturity groups and three differing sowing terms. The results of the study show that the average content of water in the grain in physiological and technological maturity, at all the hybrids and sowing terms were in 2016 significantly lower than in 2017.

The highest average yields in 2016 were achieved with hybrids of the FAO maturity group 600, in the 2^nd and 3^rd sowing term, while in 2017, the highest yields were recorded with hybrids of the FAO maturity group 500, during the 1^st and 2^nd sowing term. The average rate of dry down in 2016, in all hybrids, was the highest recorded in the 1^st sowing term, and in the year 2017, in 2^nd and 3^rd sowing term. The most intense dry down rate period in both years, at the hybrids of the FAO maturity group 400 was in the second and third week of physiological maturity, while in FAO maturity hybrids groups 500 and 600 in the third and fourth week of physiological maturity.

Key words: corn, physiological maturity, technological maturity, dry down rate, yield.

Corresponding author: Milan Biberdžić, University in Prishtina, Faculty of Agriculture, Kopaonička bb, 38232 Lešak, Serbia. Tel. +381652621147 E-mail: mbiberdzic@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 971-982

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.633.

https://doi.org/10.2298/GENSR1803971B

Original scientific paper

IDENTIFICATION OF MARKERS ASSOCIATED WITH TRAITS FOR USE

IN MARKER-ASSISTED SELECTION IN SAFFRON

Mahdi BAYAT¹, Reza AMIRNIA², Hakan OZKAN³, Aysun GEDIK³, Duygu ATES³, Mehdi RAHIMI⁴, Bahattin TANYOLAC^3*

¹Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

²Department of Agronomy and Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran.

³Department of Bioengineering, EGE University, 35040 Izmir, Turkey.

⁴ Assistant Prof., Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

Abstract

Bayat M., R. Amirnia, H. Ozkan, A. Gedik, D. Ates, M. Rahimi, B. Tanyolac (2018): Identification of markers associated with traits for use in marker-assisted selection in saffron.- Genetika, Vol 50, No.3, 971-982.

Determination of association between molecular markers and agronomic traits provides an excellent tool for marker-assisted selection. In this study, multivariate stepwise regression analysis was used to estimate associations between SSR markers and some agronomic traits in saffron ecotypes. Two-year average values for the measured traits were used for association analyses. The results of stepwise regression analysis revealed significant associations between the traits and some of the studied loci. More than one informative marker was detected for most of the traits. Totally 25 informative SSR markers were identified in two years. Markers SCA382, SCA15 and SCD219 were associated with most traits under both years. These markers are considered to be relatively more reliable. Among the SSR primers, special attention should be drawn to primers SCA382, SCA15, and SCD219, which had the highest associated fragments with most traits in two years and could be considered for use as candidate markers in marker-assisted selection.

Key words: association analysis, informative marker, regression, saffron, SSR.

Corresponding author: Bahattin Tanyolac, Department of Bioengineering, EBILTEM Institute, EGE University, Izmir, Turkey. Email: bahattin.tanyolac@ege.edu.tr

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 983-994

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803983R

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY AMONG HIGH YIELDING SELECTED SALIX CLONES, USING RAPD AND SSR MARKERS

Rameez RAJA¹, Naresh Bahadur SINGH²,Sheeraz Saleem BHAT³

¹Department of Tree Improvement and Genetic Resources, Dr. Y. S. P. University

of Horticulture and Forestry, Nauni, Solan 173230, HP, India

²College of Post Graduate Studies, Central Agricultural University, Barapani, (Meghalaya)

³ICAR-Indian Grassland and Fodder Research Institute, Jhansi-284003, Uttar Pradesh

Abstract

Raja R., N. B. Singh,S. S. Bhat (2018): Assessment of genetic diversity among high yielding selected Salix clones, using RAPD and SSR markers.- Genetika, Vol 50, No.3, 983-994.

Willows are ecofriendly, fast growing, multipurpose, widely distributed species throughout the world and improvement in breeding programs for biomass and wood quality production is the need of the hour, which can be achieved throughout hybridization programs amongst the desired clones. In this study, molecular characterization, using RAPD and SSR markers, was carried out for the 33 clones, of which 25 clones developed through crossing were more productive with respect to biomass characteristics and the other 8 were check clones. Each RAPD generated a unique set of amplification products ranging in size from 100-2500bp, the total number of alleles ranged from 6 (Deca-7) to 10 (OPO-16). All the eight primers yielded 66 scorable bands with an average band of 8.25 per primer. Of the 10 SSRs used, only eight primers produced SSR profiles with intense banding pattern, which showed some polymorphism between 33 accessions used in the study. Each marker generated a unique set of amplification products. The Jaccard’s coefficient values ranged from 0.13 to 0.76 based on RAPD and 0.39 to 0.93 based on SSR Markers. The results indicated fair range of variability in the similarity coefficient values suggesting a wide genetic base of thirty three clones taken in the experiment. Both RAPD and SSR analysis revealed high levels of genetic diversity within the reference set of willow clones. Based upon RAPD markers use, clone 14 [PN227 (S.matsudana) ×NZ1140 (S. matsudana x S. alba)] appeared to be most diverse from rest of genotype on the basis of dendrogram. To achieving better genetic gains through hybridisation programs for different desired traits in the species further research work will be helpful.

Keywords: clones, diversity, hybridization, molecular markers, Salix.

Corresponding author: Rameez Raja,Department of Tree Improvement and Genetic Resources, Dr. Y. S. P. University of Horticulture and Forestry, Nauni, Solan 173230, HP, India, Mobile No. +91-9622678012, reshirameez05@gmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 995-1010

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803995C

Original scientific paper

VARIABILITY IN MINERAL COMPOSITIONS, YIELD AND YIELD CONTRIBUTING TRAITS OF STEM AMARANTH (Amaranthus lividus)

Tonmoy CHAKRABARTY¹, Umakanta SARKER^1*, M. HASAN¹, and M. M. RAHMAN²

¹Department of Genetics and Plant Breeding, Faculty of Agriculture,Bangabandhu Sheikh Mujibur Rahman Agricultural University,Gazipur-1706, Bangladesh

²Department of Soil Science, Faculty of Agriculture,Bangabandhu Sheikh Mujibur Rahman Agricultural University,Gazipur-1706, Bangladesh

Abstract

Chakrabarty T., U. Sarker, M. Hasan, and M. M. Rahman (2018): Variability in mineral compositions, yield and yield contributing traits of stem amaranth (Amaranthus lividus).- Genetika, Vol 50, No.3, 995-1010.

Twenty stem amaranth genotypes collected previously from different eco-geographic regions of Bangladesh were assessed to evaluate variations in mineral compositions, yield and yield components, their interrelationships and direct and indirect effects on biological yield. Analysis of variance revealed significant difference among the genotypes for all the characters studied. Considering mean, range and all genetic parameters, selection could be performed on the basis of leaves per plant, leaf area, shoot weight, root weight, stem weight, Zn, Mn, Cu, Fe and biological yield for significant improvement of stem amaranth genotypes. Correlation revealed that stem base diameter, shoot weight, root weight, stem weight and leaves plant^-1 could significantly improve the biological yield of stem amaranth. Insignificant associations amongst mineral compositions indicated that improvement of mineral compositions was possible without compromising the loss of biological yield of stem amaranth. Path analysis revealed that direct selection based on shoot weight and root weight would be effective for yield improvement of stem amaranth. SA8 had higher yield along with calcium, magnesium, potassium, iron, manganese and zinc content and could be utilized as high yield potential mineral enriched variety. The genotypes SA1, SA2, SA3, SA5, SA7, SA8, SA13, SA18 and SA20 could be utilized in future breeding program for improvement of stem amaranth. The genotypes SA6 and SA11 might also be selected as a donor parent for introgression of potential genes of high minerals into other genotypes.

Keywords: Minerals, yield and yield contributing traits, variability, correlation and path coefficient, stem amaranth

Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture,Bangabandhu Sheikh Mujibur Rahman Agricultural University,Gazipur-1706, Bangladesh, Postal Code 1706. Phone: +880-1716606098, Email address: umakanta@bsmrau.edu.bd

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 1011-1021

Serbia

UDC 575

https://doi.org/10.2298/GENSR1803011D

Original scientific paper

LASER CAPTURE MICRODISSECTION OF PIGMENT CELLS FROM SALMONID FISH SKIN TO IDENTIFY DIFFERENTIALLY EXPRESSED GENES IN AN ENRICHED CELL POPULATION

Ida DJURDJEVIČ, Simona SUŠNIK BAJEC^*

Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia

Abstract

Djurdjevič I. and, S. Sušnik Bajec (2018): Laser capture microdissection of pigment cells from salmonid fish skin to identify differentially expressed genes in an enriched cell population.- Genetika, Vol 50, No.3,1011-1021.

In order to determine the function of pigment cell types in different pigment pattern of skin, we have applied the laser capture microdissection method (LCM) for isolation of different pigment cell types from skin sections of marble trout (Salmo marmoratus) and brown trout (S. trutta), characterized with labyrinthine and spotted skin pattern, respectively. RNA was isolated from dissected pigment cell populations and used for expression analysis. Expression profiles of candidate genes, potentially involved in pigmentation, in LCM isolated pigment cell population, were more specific compared to differently pigmented skin regions, containing a heterogeneous mixture of cells, including more types of chromatophores. Moreover, they could provide important information about particular pigment cell type function and its contribution to different pigment pattern. Protocol of combined LCM and genomic approach to study single pigment cell populations can be applied in the future, but needs to be further optimized in order to yield RNA of sufficient quantity and quality required for i.e. transcriptomic analyses.

Key words: LCM, Salmo trutta, Salmo marmoratus, pigment cell, RNA expression

Corresponding author: Simona Sušnik Bajec, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia. T: + 386 1 320 3944; F: + 386 1 724 10 05; E-mail: simona.susnik@bf.uni-lj.si

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp.1023-1033

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1803023M

Original scientific paper

ANTIOXIDANT STATUS OF THE DIFFERENT SWEET MAIZE HYBRIDS

UNDER HERBICIDE AND FOLIAR FERTILIZER APPLICATION

Jelena MESAROVIĆ^1*, Jelena SRDIĆ¹, Snežana mladenović drinić¹, Vesna DRAGIČEVIĆ¹, Milena simić¹, Milan brankov¹ and Dušanka milojković-opsenica²

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²University of Belgrade - Faculty of Chemistry, Belgrade, Serbia

Abstract

Mesarović J., J. Srdić, S. mladenović drinić, V. Dragičević, M. simić, M. brankov and D. milojković-opsenica (2018): Antioxidant status of the different sweet maize hybrids under herbicide and foliar fertilizer application.- Genetika, Vol 50, No.3, 1023-1033.

The chemical method of weed control is an indispensable step in cropping practices of sweet maize. Application of the herbicides can induce the abiotic stress which affects the non-enzymatic antioxidants in the crops, especially on the sensitive one, like sweet maize is. Antioxidant profile, through the measurement of the soluble phenolic, carotenoids, phytic acid and glutathione concentration, in the grain of the three sweet maize hybrids after application of herbicides, foliar fertilizer, as well as their combinations, in field experiment, conducted over a two-year period, was determined. The content of tested antioxidant parameters was dependent on hybrids, growing season, as well as of the applied treatment. Sulfonylurea herbicides significantly increased the antioxidant status of sweet maize fresh grain, compared to the herbicide from triketone group, without affecting the fresh grain yield. Combination of herbicide plus foliar fertilizer expressed a various impact on antioxidant profile of the maize grain. Furthermore, significant correlations (positive and negative) between fresh grain yield and analyzed antioxidants in grain of three sweet maize hybrids were noticed.

Keywords: non-enzymatic antioxidant, Zea mays L. saccharata, abiotic stress, nicosulfuron, mesotrione

Corresponding author: Jelena Mesarović, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, e-mail: jmesarovic@mrizp.rs

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Published in „GENETIKA“ Vol. 50, No. 3(2018), pp. 1035-1044

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR18036035S

Original scientific paper

PHYLOGENETIC ANALYSIS OF SOME Citrus L. (RUTACEAE) TAXA IN TURKEY BASED ON CHLOROPLAST (cpDNA) trnL INTRON AND trnL-F DNA SEQUENCES

Emre SEVİNDİK^*and Kübra YALÇIN

Department of Agricultural Biotechnology, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey

Abstract

Sevindik E.and K. Yalçin (2018): Phylogenetic analysis of some Citrus L. (Rutaceae) taxa in Turkey based on chloroplast (cpDNA) trnL intron and trnl-f DNA sequences.- Genetika, Vol. 50, No. 3, 1035-1044.

In this study, phylogenetic analysis of some Turkish Citrus species was conducted based on chloroplast DNA (trnL intron and trnL-F) sequences. Citrus taxa were plotted on a phylogenetic tree where Zanthoxylum ailanthoides was used as the outgroup.The sequences for trnL intron and trnL-F regions of the outgroup were retrieved from NCBI GenBank. All plant samples were collected from different locations during the inflorescence and vegetation periods, and brought to the laboratory. Genomic DNA was isolated from healthy green leaves using DNAeasy Plant Mini Kit. trnL intron region was amplified using universal primers trnc and trnd, while for the trnL-F region trne and trnf were used. Later obtained DNA sequences were edited using BioEdit 7.0.4.1 and FinchTV. Sequencing data were aligned via ClustalW program and analyzed using MEGA 6.0 software. Maximum Likelihood and bootstrap trees were constructed in order to identify the relationships among Citrus taxa. The trnL intron sequences ranged from 554 to 581 nucleotides. The average nucleotide composition of trnL intron was 26.3% T, 16.8% C, 37.7% A and 19.2% G. The divergence values varied from 0.000 to 0.057. The trnL-F sequences ranged from 392 to 399 nucleotides. Average nucleotide composition was 34.3% T, 21.2% C, 27.4% A and 17.1% G, while the divergence values of trnL-F sequences varied from 0.000 to 0.003. As a result, trees constructed based on trnL intron region have systematically been found to show more reliable and compatible results than those formed based on the trnL-F region.

Keywords: Citrus, cpDNA, trnL intron, trnL-F, phylogenetic analysis.

Corresponding author: Emre Sevindik, Department of Agricultural Biotechnology College of Agriculture, Adnan Menderes University,South Campus, 09970 Cakmar-Aydin- Turkey, Phone: + 90 (256) 772 71 48 ext: 2529,e-mail: ph.d-emre@hotmail.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp 1045-1054

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1803046C

Original scientific paper

EVALUATION OF DIFFERENT SIGMOIDAL GROWTH MODELS AND CLIMATE PARAMETERS FOR DRY MATTER ACCUMULATION OF OAT

Yalçın COŞKUN

Çanakkale Onsekiz Mart University, Lapseki Vocational College, Çanakkale, Turkey

Abstract

Coşkun Y. (2018): Evaluation of different sigmoidal growth models and climate parameters for dry matter accumulation of oat.- Genetika, Vol 50, No.3, 1045-1054.

The monitoring of the biological growth of field crops is important for planning and scheduling agricultural applications. In order to assess biological growth pattern and, dry matter accumulation of Yeniçeri oat variety were obtained in Çanakkale conditions in 2012-2013 and 2013-2014 growing seasons with continuous plant samplings from seedling emergence until seed maturation. Gompertz, Logistic, Logistic Power, Weibull, and Ratkowsky sigmoidal growth models are fitted to actual growth data and their predictions were compared. Results suggested that all sigmoidal growth models successfully explained oat dry matter accumulation a high R²values (over 99%) and low mean square errors, Weibull model fitted lower than others for first year with an R²value under 99%. Dry matter accumulation was also investigated as a result of average temperature and precipitation with stepwise regression. Results indicated that average weather temperature has a similar pattern across both growing seasons and has a major influence on dry matter accumulation.

Keywords: dry matter, growth models, oat, stepwise regression, temperature

Corresponding author: Dragan Niksic, Institute for Animal Husbandry, Autoput 16, 11080, Belgrade-Zemun, Republic of Serbia, e-mail: draganniksic84@gmail.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 1055-1065

UDC 575.630.3

https://doi.org/10.2298/GENSR1803055R

Original scientific paper

EFFECT OF MOTHER’S AGE ON BODY WEIGHT OF LAMBS FROM BIRTH TO WEANING IN VARIOUS STRAINS OF DOMESTIC SHEEP PRAMENKA

Bojana RISTANOVIĆ*, Zoran. Ž. ILIĆ

University of Pristina, Faculty of Agriculture, Kosovska Mitrovica-Lesak,

Kosovo and Metohija, Serbia

Abstract

Ristanovic, B., Z.Ž. Ilić (2018): Effect of mother’s age on body weight of lambs from birth to weaning in various strains of domestic sheep pramenka. - Genetika, Vol 50, No. 3, 1055-1065.

This study has been conducted on the domestic sheep Pramenka that is a dominant breed in Serbia. The purpose is to determine the effects of mother’s age on suckling and growth of lambs, and to find out at which week the lambs are normally weaned. Research was carried out on 1800 lambs of four strains (Svrljiški, Pirotski, Sjenički, Šarplaninski) of sheep Pramenka (450 lambs per strain) for body weight. The weight of lambs was measured at the time of birth, 30, 60 and 90 days after birth during three years of experiment (2011-2013). Obtained results refer to variability of body weight of lambs at the time of birth, 30, 60 and 90 days after birth depending on mother’s age within each of four strains of Pramenka sheep. At the time of birth, the highest average weight (3.79 kg) of lambs was found in second age group of Pirotska genotype, while the highest average weight in third age group was found in remaining three Svrljiška (3.91 kg), Sjenička (3.74 kg) and Šarplaninska (3.97 kg) strain of Pramenka sheep. Thirty days after birth the highest average weight of lambs was found in the second age group of Pirotska genotype (9.36 kg), in the first age group in Svrljiška (9.89 kg), while the highest average weight in third age group was found in remaining two Sjenička (9.51 kg) and Šarplaninska (9.41 kg) strain of Pramenka sheep. Sixty days after birth, the highest average weight of lambs was found in third age group of Pirotska genotype (15.85 kg), in second age group in Svrljiška (15.51 kg) and Sjenička (15.50 kg) genotypes, while the highest average weight in first age group was found in Šarplaninska (14.45 kg) strain of Pramenka sheep. Ninety days after birth, the highest average weight of lambs was found in second age group of Pirotska (20.58 kg) and Šarplaninska (20.45 kg) genotype, while the highest average weight in first age group was found in Svrljiška (24.38 kg) and Sjenička (23.76 kg) strain of Pramenka sheep. The variability in body weight of lambs at birth, as well at different stages of growth after weaning at early growth stages is influenced by direct maternal genotype, strains of Pramenka sheep and age of ewes.

Keywords: Pramenka sheep, body weight of lamb, mother’s age

Corresponding author: Bojana Ristanović, University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lešak, Kosovo and Metohia, Serbia, e-mail: bojana.ristanovic@yahoo.com

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Published in „GENETIKA“ Vol. 50, No. 3 (2018), pp. 1067-1080

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1803067B

Original scientific paper

EVALUATION OF MAIZE GRAIN YIELD AND YIELD STABILITY BY AMMI ANALYSIS

Dragana BRANKOVIĆ-RADOJČIĆ^1*, VOJKA BABIĆ¹, Zdenka GIREK², Tomislav ŽIVANOVIĆ³, Aleksandar RADOJČIĆ⁴, Milomir FILIPOVIĆ¹, Jelena SRDIĆ¹

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²Institute for vegetable crops, Smederevska Palanka, Serbia

³Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

⁴Chemical Agrosava, Belgrade, Serbia

Abstract

Branković-Radojčić D., V. Babić, Z. Girek, T. Živanović, A. Radojčić, M. Filipović, J. Srdić (2018): Evaluation of maize grain yield and yield stability by AMMI analysis.- Genetika, Vol 50, No.3, 1067-1080.

Significant genotype x environment interaction for quantitative traits, such is grain yield, reduces the usefulness of genotype means, over all environments, for selecting superior genotypes. AMMI model is a valuable statistical tool in identifying systemic variation contained in the interaction effect. Obtained data could be applied in maximizing yield potential in every environment based on both narrow and wide genotype adaptability, without the necessity of developing breeding programs for smaller targeted environments. Precise assortment of superior genotypes, with the assistance of AMMI model, leads to the better recommendation of newly bred hybrids, and thus increasing maize grain yield in a targeted environment. In this research genotype x environment interaction and yield stability of 36 maize hybrids of FAO 300-700 maturity group was investigating. The trial was set according to Randomized Complete Block Design (RCBD). Data were processed in order to obtain average estimates of grain yield, and yield stability was assessed by the method of AMMI analysis. The highest average grain yield was achieved in 2011 (11.62 t/ha), and the lowest in the most stressful and dry 2012 (6.90 t/ha). In the region Loznica L2 the highest average yield was noticed (13.81 t/ha), while at L7 (Sremska Mitrovica) average grain yield was the lowest (6.97 t/ha). Results of AMMI analysis gave precise recommendation for production of maize hybrids in certain environments, by determining winning areas of hybrids H20, H11 and H36. Medium early maturing and high yielding hybrids (H11 and H20) are therefore considered more favorable for production in environments with lower precipitation, while high yielding and more stable hybrids H21 and H35 are suitable for a wider range of environments. Hybrid H36 (FAO 700) showed its full potential at L2, and L3 which did not suffer from a lack of moisture. This hybrid also expressed its best potential in environments with favorable conditions.

Key words: AMMI model, GxE interaction, maize grain yield, yield stability

Corresponding author: Dragana Branković-Radojčić, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia; Tel: +38111 37 56 704; Fax: +38111 37 56 707;

e-mail: dbrankovic@mrizp.rs; jsrdic@mrizp.rs

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Published in „GENETIKA“ Vol. 50, No. 3(2018), pp. 1081-1094

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1803081M

Original scientific paper

NON-PARAMETRIC APPROACH TO THE ANALYSIS OF PHENOTYPIC STABILITY OF TWO HALF-SIB MAIZE POPULATIONS

Bojan MITROVIĆ¹*, Dušan STANISAVLJEVIĆ¹, Filip FRANETA¹, Sanja MIKIĆ¹,

Petar ČANAK¹, Bojana VUJOŠEVIĆ¹, Emilija NIKOLIĆ-ĐORIĆ²

¹Institute of Field and Vegetable Crops, Novi Sad

²University of Novi Sad, Faculty of Agriculture, Novi Sad

Abstract

Mitrović B., D. Stanisavljević, F. Franeta, S. Mikić, P. Čanak, B. Vujošević, E. Nikolić-Đorić (2018): Non-parametric approach to the analysis of phenotypic stability of two half-sib maize populations.- Genetika, Vol 50, No.3, 1081-1094.

One of the most important phases in commercial maize breeding programs is the assessment of the value of newly-developed progeny by testing in hybrid combinations. In this study, non-parametric stability measures were applied to analyze the genotype × environment interaction and to assess phenotypic stability of two half-sib maize populations, each consisting of 40 genotypes, across 9 variable environments. Non-parametric tests of significance determined the presence of qualitative interaction for grain yield in both observed populations. Results of the stability analysis showed no significant differences between the two progeny groups indicating that the used testers did not bring significant increase in stability in either of the analyzed half-sib populations. Individual genotypes were also compared based on grain yield stability within both progeny groups using the stability parameters S_i⁽¹⁾, S_i⁽²⁾, S_i⁽³⁾and S_i⁽⁶⁾. Association between the grain yield and stability indices S_i⁽¹⁾and S_i⁽²⁾of the analyzed genotypes was presented graphically enabling the identification of genotypes which can be recommended for further breeding process as the most promising ones. The correlations between grain yield and stability parameters were tested by Spearman’s rank correlations. Both progeny groups (HS1 and HS2) showed no significant correlations between the grain yield and stability parameters S_i⁽¹⁾and S_i⁽²⁾, but the rank correlations between S_i⁽¹⁾and S_i⁽²⁾ values were very strong and highly significant. Highly significant negative correlations were found between grain yield and stability indices S_i⁽³⁾and S_i⁽⁶⁾in both progeny groups, and very strong and highly significant correlations were found between S_i⁽³⁾and S_i⁽⁶⁾ values.

Key words: genotype by environment interaction, grain yield, stability indices

Corresponding author: Bojan Mitrović, Maksima Gorkog 30, 21000 Novi Sad, Serbia, phone: +381 21 4898 293, fax: +381 21 4898 250, e-mail: bojan.mitrovic@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 50 No. 3 (2018), pp. 1095-1109

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1803095K

Review paper

ACHIEVEMENTS AND PROSPECTS OF MOLECULAR BREEDING FOR DROUGHT TOLERANCE IN SOYBEAN [Glycine max (L.) MERR.]

Mueen Alam KHAN

¹Department of Plant Breeding and Genetics, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

Abstract

Khan M. A. (2018): Achievements and prospects of molecular breeding for drought tolerance in soybean [Glycine max (L.) merr.].- Genetika, Vol 50, No.3, 1095-1109.

Drought stress is one of the most serious constraints of soybean production worldwide. While the drought tolerance is one of the most complex attributes for soybean breeders to deal with. Due to complex (quantitative) nature of drought tolerance trait, the progress to develop drought tolerant plants is slow. Molecular breeding thus emerged as a necessary approach in soybean breeding programs. A plethora of genetic information regarding the functional genomics and other molecular resources is available. The effective and astute use of these resources will certainly facilitate the breeders to develop soybean cultivars tolerant to drought.

Keywords: Drought stress, Glycine max L., molecular breeding, soybean.

Corresponding author: Mueen Alam Khan, Department of Plant Breeding and Genetics, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan, E-mail: mueen_1981@yahoo.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 1111-1121

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803111B

Review paper

GENERAL CHARACTERISTICS OF THE MELANOGENESIS PROCESS WITH PARTICULAR EMPHASIS ON THE ROLE OF THE PAX3 GENE

Jan BIŃKOWSKI, Marek KMIEĆ, Jakub KUBIŚ

Department of Genetics and Animal Breeding, Faculty of Biotechnology and Animal Husbandry

West Pomeranian University of Technology, Szczecin, Poland

Abstract

Bińkowski J., M. Kmieć, J. Kubiś (2018): General characteristics of the melanogenesis process with particular emphasis on the role of the PAX3 gene.- Genetika, Vol 50, No.3, 1111-1121.

Melanogenesis is a dynamic process of biological transformations leading to the formation of copolymeric dyes - melanins, which act as a protector against the ultraviolet radiation (UV) and reactive oxygen species (ROS). The process of these compounds formation is carried out by an enzymatic apparatus in specialized melanocyte organelles – melanosomes. Particularly from the point of view of biochemistry, melanins synthesis occurring depend to a large extent not only on genetic but also on environmental conditions. Pax3 is an important candidate in research on genetic conditioning of animal colors pattern due to the fact that his gene expression product is a highly conserved transcription factor that during embryonic development is one of the elements responsible for regulating stem cell differentiation of the neural crest into melanocytes. Moreover during individual life Pax3 is involved in the response of melanocytic units to UV radiation. Mutations/polymorphisms of the Pax3 gene are the cause of the occurrence of some color varieties, as well as developmental disorders.

Keywords: Pax3, melanogenesis, melanocytes, melanoblast, coat color.

Corresponding author: Jan Bińkowski, Department of Genetics and Animal Breeding, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland, E-mail: jan.binkowski96@gmail.com

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Published in „GENETIKA“ Vol. 50, No.3 (2018), pp. 1123-1135

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1803123P

Review paper

A REVIEW ON ANTIBIOTIC RESISTANCE: ORIGIN AND MECHANISMS OF BACTERIAL RESISTANCE AS BIOLOGICAL PHENOMENON

Svetlana PEROVIĆ¹, Gorana VEINOVIĆ²,Jelena ANTIĆ STANKOVIĆ^3*

¹University of Montenegro, Faculty of Natural Sciences and Mathematics, Department of Biology, Podgorica, Montenegro;

²University of Belgrade, Institute for Medical Research, Belgrade, Serbia;

³University of Belgrade, Faculty of Pharmacy, Department of Microbiology and Immunology, Belgrade, Serbia

Abstract

Perović S., G. Veinović,J. Antić Stanković (2018): A review on antibiotic resistance: origin and mechanisms of bacterial resistance as biological phenomenon.- Genetika, Vol 50, No.3,1123-1135.

Antimicrobial resistance is a natural biological phenomenon in their struggle for existence and represents a major health care issue, associated with high mortality and morbidity. The first cases of mass emergence of resistant strains were observed in the middle of the 20th century, and since then cases of resistance have been reported all over the world, and in the last two decades even more frequently are reported multiple bacterial resistance. Factors that contribute to the development of bacteria resistance are abuse in the use of antibacterial agents, in humans or livestock, and releases of antibacterial agents into the environment. Moreover, the development of new effective antibiotics is decreasing, contrary to increasing the overall effort for the synthesis of new ones. Identification and reporting of bacterial resistance, as well as monitoring of the use of antibiotics in the outpatient and inpatient setting, today is the obligation of all countries.

Keywords: bacterial resistance, mechanisms, metagenomics, natural products, P. aerugonosa, S. aureus, M. tuberculosis, Acinetobacter

Corresponding author: Jelena Antić Stanković, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11 000 Belgrde, Serbia, +381 11 3951224, jelena@pharmacy.bg.ac.rs

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