GENETIKA, Vol

GENETIKA, Vol. 51, No. 1 (2019)

Aleksandra SAVIĆ, Milka BRDAR-JOKANOVIĆ, Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Milan ZDRAVKOVIĆ, Dalibor ŽIVANOV, Mirjana VASIĆ

GENETIC DIVERSITY OF COMMON BEAN (Phaseolus vulgaris L.) BREEDING COLLECTION IN SERBIA [Abstract] [Full text]

Yousef NADERI

ESTIMATION OF GENETIC PARAMETERS FOR BODY WEIGHT TRAITS IN MAZANDARAN NATIVE BREEDER HENS BY RANDOM REGRESSION MODELS [Abstract] [Full text]

Marko FLAJŠMAN, Igor ŠANTAVEC, Aleš KOLMANIČ, Katarina KOŠMELJ, Darja Kocjan AČKO

AGRONOMIC PERFORMANCE AND STABILITY OF SEED, PROTEIN AND OIL YIELDS OF SEVEN SOYBEAN CULTIVARS DETERMINED IN FIELD EXPERIMENTS IN SLOVENIA [Abstract] [Full text]

Maja MATOŠA KOČAR, Sonja VILA, Sonja PETROVIĆ, Andrijana REBEKIĆ, Aleksandra SUDARIĆ³, Tomislav DUVNJAK,Antonela MARKULJ KULUNDŽIĆ,Ana JOSIPOVIĆ
ISOFLAVONE CONCENTRATIONS IN SOYBEANS SUITABLE FOR GROWING IN EUROPE [Abstract] [Full text]

Karel VEHOVSKÝ, Kateřina ZADINOVÁ, Roman STUPKA, Jaroslav ČÍTEK, Monika OKROUHLÁ, Nicole LEBEDOVÁ, Michal ŠPRYSL

EFFECT OF THE SER638ARG VARIATION IN THE CAST GENE AND CAUSAL SNP G.1843C>T IN THE RYR1 GENE ON CARCASS TRAITS IN CROSBREED PIGS [Abstract] [Full text]

Emre SEVİNDİK

COMPARATIVE AND PHYLOGENETIC ANALYSIS OF RUBISCO LARGE SUBUNIT (rbcL) PROTEINS IN SOME Sideritis L. (LAMIACEAE) SPECIES: A BIOINFORMATIC APPROACH [Abstract] [Full text]

Sandra CVEJIĆ, Siniša JOCIĆ, Velimir MLADENOV, Borivoje BANJAC, Ilija RADEKA, Milan JOCKOVIĆ, Ana JEROMELA MARJANOVIĆ, Dragana MILADINOVIĆ, Vladimir MIKLIČ

Selection of sunflower hybrids based on stability across environments [Abstract] [Full text]

Klára ŠTIASNA, Mária PRESINSZKÁ, Tomáš VYHNÁNEK, Václav TROJAN, Pavel HANÁČEK, Ladislav HAVEL

SEQUENCE ANALYSIS OF FLAVANONE 3-HYDROXYLASE AND DIHYDROFLAVONOL 4-REDUCTASE GENES IN WHEAT WITH NONSTANDARD COLOURED CARYOPSES [Abstract] [Full text]

Ali Hussein ABU-ALMAATY, Mohamed K. HASSAN, Neveen E. R. EL BAKARY, Sarah H. AHMED

CHROMOSOMAL EVOLUTION AND MOLECULAR GENETIC ANALYSIS OF FOUR SPECIES OF GENUS ANAS (AVES: ANATIDAE) [Abstract] [Full text]

Branislava BATOS, Danijela MILJKOVIĆ

THE PHENOTYPIC PLASTICITY OF of Picea omorika /Panč./Purkyne MORPHOLOGICAL POLLEN TRAITS [Abstract] [Full text]

Martin VIVODÍK, Želmíra BALÁŽOVÁ, Zdenka GÁLOVÁ and Lenka PETROVIČOVÁ

START CODON TARGETED POLYMORPHISM FOR EVALUATION OF FUNCTIONAL GENETIC VARIATION AND RELATIONSHIPS IN CULTIVATED CASTOR (Ricinus communis L.) GENOTYPES [Abstract] [Full text]

Mahdiyeh YOUSEFI DARESTANI, Seyed-Resa MIRAEI ASHTIANI, Mostafa SADEGHI

STUDY OF GENETIC DIVERSITY OF IRANIAN INDIGENOUS BUFFALO POPULATIONS USING MICROSATELLITE MARKERS [Abstract] [Full text]

Radovan MILIĆEVIĆ, Ljiljana BRANKOVIĆ, Desanka RADULOVIĆ, Dragana JUGOVIĆ, Hristina STAMENKOVIĆ, Tatjana STANKOVIĆ, Aleksandar MILIĆEVIĆ, Višnja MADIĆ, Marina RISTIĆ

FETAL CHROMOSOMAL ANOMALIES IN SOUTHEAST SERBIA- SINGLE CENTER COHORT RETROSPECTIVE STUDY [Abstract] [Full text]

Velimir MLADENOV, Miodrag DIMITRIJEVIĆ,Sofija PETROVIĆ, Jan BOĆANSKI, Borislav BANJAC, Ankica KONDIĆ-ŠPIKA, Dragana TRKULJA

GENETIC analysis of SPIKE length IN wheat [Abstract] [Full text]

Sesilia Anita WANGET, María Remedios Morales-Corts, Rodrigo Pérez-Sánchez, Neni ROSTINI, María Ángeles Gómez-Sánchez, Agung KARUNIAWAN

Agro-morphological and chemical characterization of traditional Indonesian peanut (Arachis hypogaea L.) cultivars [Abstract] [Full text]

Manisha THAKUR, Ramesh KUMAR, Sandeep KANSAL

HETEROSIS, COMBINING ABILITY AND GENE ACTION STUDIES IN CUCUMBER FOR DIFFERENT BIOTIC STRESSES TO DEVELOPE RESISTANT HYBRIDS [Abstract] [Full text]

Pavle ERIĆ, Mihailo JELIĆ, Marija SAVIĆ VESELINOVIĆ, Bojan KENIG, Marko ANĐELKOVIĆ, Marina STAMENKOVIĆ-RADAK

NUCLEOTIDE DIVERSITY OF Cyt b GENE IN Drosophila subobscura Collin [Abstract] [Full text]

Lawrence A. AKINRO, Adenubi I. ADESOYE, Taiye R. FASOLA

GENETIC DIVERSITY IN Cola acuminata AND Cola nitida USING RAPD PRIMERS [Abstract] [Full text]

Ana NIKOLIĆ, Natalija KRAVIĆ, Jelena SRDIĆ, Dragan KOVAČEVIĆ,Violeta ANĐELKOVIĆ, Milomir FILIPOVIĆ, Snežana MLADENOVIĆ DRINIĆ

DIVERGENCE AMONG MAIZE GENOTYPES WITH DIFFERENT KERNEL TYPES ACCORDING TO SSR MARKER ANALYSIS [Abstract] [Full text]

Amri YUDHISTIRA and dan Tuty ARISURYANTI

PRELIMINARY FINDINGS OF CRYPTIC DIVERSITY OF THE GIANT TIGER SHRIMP (Penaeus monodon Fabricius, 1798) IN INDONESIA INFERRED FROM COI MITOCHONDRIAL DNA [Abstract] [Full text]

Dong ZHU, Yanlin LIU, Man JIN, Guanxing CHEN, Slaven PRODANOVIC, Yueming YAN

EXPRESSION AND FUNCTION ANALYSIS OF WHEAT EXPASIN GENES EXPA2 AND EXPB1 [Abstract] [Full text] [Supplement]

Valentin KOSEV and Viliana VASILEVA

Comparative biological characteristic of white lupin (Lupinus albus L.) varieties [Abstract] [Full text]

Dragana BOŽIĆ, Markola SAULIĆ, Aleksandra SAVIĆ, George GIBBINGS, Sava VRBNIČANIN

Studies on gene flow from herbicide resistant to weedy sunflower [Abstract] [Full text]

Yasmin M. Abd EL-AZIZ, Ali H. ABU-ALMAATY, Nahed A.OMAR, Ahmed M. ABDEEN, Mahmmoud M. ZAKARIA

EVALUATION OF PROTECTIVE EFFECTS OF PROPOLIS AGAINST ALUMINIUM SILICATE TOXICITY IN RATS [Abstract] [Full text]

Zeynep GÖKKUŞ and Mevlüt AKÇURA

AN ALTERNATIVE STATISTICAL MODEL FOR THE ASSESSMENT OF DRY MATTER ACCUMULATION IN COOL SEASON CEREALS: COX REGRESSION [Abstract] [Full text]

B. M. BOJINOV

QUANTITATIVE TRAIT LOCI DETECTION IN BULGARIAN COTTON MUTANT SEGREGATING POPULATION [Abstract] [Full text]

Biljana ROGIĆ, Božo VAŽIĆ, Mihajla ĐAN, Marina STAMENKOVIĆ RADAK

GENETIC DIVERSITY AND STRUCTURE OF AUTOCHTHONOUS CATTLE BREEDS FROM BOSNIA AND HERZEGOVINA BASED ON MICROSATELLITES [Abstract] [Full text]

Jelena GVOZDENOVIĆ-JEREMIĆ and Ljiljana MOJOVIĆ

DRUG REPOSITIONING FOR A RARE GENETIC DISORDER PROGRESSIVE OSSEOUS HETEROPLASIA (POH) [Abstract] [Full text]

Milica NIKOLIĆ, Iva SAVIĆ, Ana NIKOLIĆ, Goran STANKOVIĆ, Nenad DELIĆ, Snežana MLADENOVIĆ DRINIĆ, Slavica STANKOVIĆ

Maize resistance to ear rot caused by aspergillus parasiticus [Abstract] [Full text]

Dragoslav MARINKOVIĆ

IN MEMORIAM

Academician Ljubomir Berberović [Text]

Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 1-15

UDC 575.633

https://doi.org/10.2298/GENSR1901001S

Orginal scientific paper

GENETIC DIVERSITY OF COMMON BEAN (Phaseolus vulgaris L.) BREEDING COLLECTION

IN SERBIA

Aleksandra SAVIĆ¹, Milka BRDAR-JOKANOVIĆ^1*, Miodrag DIMITRIJEVIĆ²,

Sofija PETROVIĆ², Milan ZDRAVKOVIĆ³, Dalibor ŽIVANOV¹, Mirjana VASIĆ¹

¹Institute of Filed and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

³Institute of Soil Science, Belgrade, Serbia

Abstract

Savić A., M. Brdar-Jokanović, M. Dimitrijević, S. Petrović, M. Zdravković, D. Živanov, M. Vasić (2019): Genetic diversity of common bean (Phaseolus vulgaris L.) breeding collection in Serbia.- Genetika, Vol 51, No.1, 1-15.

The characterization of 41 common bean cultivars and landraces from breeding collection of Institute of Field and Vegetable Crops, Novi Sad, Serbia, was done based on phenotypic traits and microsatellite markers. Phenotypic traits were chosen from Bioversity International descriptor list. In addition, main yield components were investigated. Analysis of phaseolin type revealed affiliation of cultivars and landraces to Mesoamerican or Andean gene pool. Cultivars and landraces demonstrated significant diversity level with regard to studied phenotypic traits. Identified variation showed high potential for developing new cultivars with desirable combination of traits. Principal component analysis based on phenotypic traits separated bean cultivars and landraces in two groups, which corresponded to Mesoamerican and Andean determined according to phaseolin type. Putative hybrids, with combination of traits between gene pools were also identified. Analysis of microsatellite data, using twenty-two SSR primer pairs, showed medium gene diversity in studied material. Microsatellite-based cluster analysis separated genotypes in two discrete clusters and several subclusters. No clear separation according to gene pool was found between the clusters, however grouping according to gene pool and patterns of phenotypic variation, following these gene pools, were observed within subclusters. Knowledge on detailed relationships of cultivars and landraces based on phenotypic and molecular data would facilitate identification of candidates for future breeding.

Keywords: phaseolin, Phaseolus vulgaris, phenotypic diversity, molecular diversity

Corresponding author: Milka Brdar-Jokanović, Institute of Field and Vegetable Crops; Phone: +381 21 780 365, E-mail: milka.brdar@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp.17-29

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901017N

Original scientific paper

ESTIMATION OF GENETIC PARAMETERS FOR BODY

WEIGHT TRAITS IN MAZANDARAN NATIVE BREEDER HENS

BY RANDOM REGRESSION MODELS

Yousef NADERI

Department of Animal Science, Astara Branch, Islamic Azad University, Astara, IRAN

Abstract

Naderi Y. (2019): Estimation of genetic parameters for body weight traits in Mazandaran native breeder hens by random regression models.- Genetika, Vol 51, No.1, 17-29.

The primary concern of this study is to investigate appropriate random regression model for estimate genetic parameters body weight at hatch (BW1), eight (BW8), twelve (BW12) and thirty two (BW32) weeks of ages by the restricted maximum likelihood method. The body weight records set included 39872 during 16 generations of hens kept at the Mazandaran Breeding Center of Iran. Random regression were modelled using generation-hatch as a fixed effect and additive genetic and permanent environmental effects as random effects Residual variances were modeled through a step function with 1 and 3 classes. The model was considered to be the most appropriate with the highest significant log likelihood ratio test (LRT) and the lowest Akaike information criterion (AIC) and Bayesian information criterion (BIC). Heritability values increased from 0.21 for BW1, to 0.40 for BW32. Genetic correlations of body weight at different record keeping were often higher than permanent environmental correlations. Genetic correlations between pairs of body weight measures were moderate to high with a range from 0.25 to 0.97. The largest genetic correlation, as well as permanent environmental correlation, was observed between BW12and BW32. High and moderate broad sense heritability values for all studied traits shows that these traits are less influenced by residual effects which make them effectively transmitted to the progeny. Findings show that genetic improvement for body weight can be achieved by selection. The Heritability of body weight at thirty two weeks of ages and its relatively high genetic correlation with all other ages showed that it could be the most appropriate period for selection. Also, the genetic trend estimates for body weight traits showed that selection decisions made during the breeding program effectively improved the growth performance.

Keywords: Akaike information criterion (AIC), Bayesian information criterion (BIC), Mazandaran Native Breeder Hens, Random Regression Model

Corresponding author: Yousef Naderi, Department of Animal Science, Astara Branch, Islamic Azad University, Astara, Iran, Email: yousefnaderi@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp.31-46

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1901031F

Original scientific paper

AGRONOMIC PERFORMANCE AND STABILITY OF SEED, PROTEIN

AND OIL YIELDS OF SEVEN SOYBEAN CULTIVARS DETERMINED

IN FIELD EXPERIMENTS IN SLOVENIA

Marko FLAJŠMAN¹, Igor ŠANTAVEC¹, Aleš KOLMANIČ², Katarina KOŠMELJ¹,

Darja Kocjan AČKO^1*

¹Department for agronomy, Biotechnical faculty, University of Ljubljana, Ljubljana, Slovenia

²Agricultural Institute of Slovenia, Crop Science Department, Ljubljana, Slovenia

Abstract

Flajšman M., I. Šantavec, A. Kolmanič, K. Košmelj, D. Kocjan Ačko (2019): Agronomic performance and stability of seed, protein and oil yields of seven soybean cultivars determined in field experiments in Slovenia.- Genetika, Vol 51, No1., 31-46.

The environment can have a crucial influence on soybean production in particular regions, even more so when production environments are different from breeding environments. Therefore, soybean cultivars must be evaluated in field trials at various locations in order to explore the duration of the growing period and capacity of yield, which is based on interactions between genotype and environment. Seven soybean cultivars originating from five European countries were evaluated for thousand-seed weight, seed, protein and oil yields and protein and oil contents in five environments in Slovenia. Yield stability of seed, protein and oil was determined in order to select best genotype. Results showed that cultivar and environment had a significant impact on all measured variables. In almost all tested environments the best seed, protein and oil yielding cultivars were ES Mentor (3425–5628 kg seed/ha, 1280–2192 kg protein/ha, 640.8–918.9 kg oil/ha) and NS Mercury (3468–5342 kg seed/ha, 1266–2071 kg protein/ha, 618.6–880.7 kg oil/ha). The highest average protein content was found in NS Favorit (41.0% of DW). Volma had the highest average oil content (20.2%). Three yield stability indices were used to assess yield stability; in terms of Shukla’s variance (σ_i²) and S square Wricke’s ecovalence (W_i²), Josefine turned out to have most stable seed and oil yields, while ES Mentor had most stable protein yields. Regarding Kang’s yield stability (YS_i), which makes simultaneous selection for mean yield and stability and is therefore most relevant for practical usage, ES Mentor, NS Mercury and NS Favorit were selected as superior genotypes regarding stability of seed, protein and oil in this study. Correlation analysis of some chosen traits showed different interdependence between measured variables depending on the environment. As expected, protein and oil contents were negatively correlated, yet this was only significant in one environment.

The results of this study pointed out that among the seven tested cultivars, ES Mentor, NS Mercury and NS Favorit were best genotypes and are highly recommended for usage in soybean production in Slovenia.

Keywords: soybean, content of proteins and oil, yield of seed, protein and oil, yield stability indices, correlation

Corresponding author: Darja Kocjan Ačko, Department for agronomy, Biotechnical faculty, University of Ljubljana, Ljubljana, Slovenia, Phone: +386 (0)1 320 32 79, E-mail: darja.kocjan.acko@bf.uni-lj.si

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Published in „GENETIKA“ Vol. 51, No1(2019), pp. 47-59

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901047M

Original scientific paper

ISOFLAVONE CONCENTRATIONS IN SOYBEANS SUITABLE FOR GROWING IN EUROPE

Maja MATOŠA KOČAR¹, Sonja VILA², Sonja PETROVIĆ², Andrijana REBEKIĆ², Aleksandra SUDARIĆ^1,3, Tomislav DUVNJAK¹,Antonela MARKULJ KULUNDŽIĆ¹,Ana JOSIPOVIĆ¹

¹Agricultural Institute Osijek, Osijek, Croatia,

²Faculty of Agriculture in Osijek, Josip Juraj Strossmayer University of Osijek, Croatia

³Centre of Excellence for Biodiversity and Molecular Plant Breeding, University of Zagreb, Faculty of Agriculture, Zagreb, Croatia

Abstract

Matoša Kočar M., S.Vila, S.Petrović, A.Rebekić, A.Sudarić, T.Duvnjak,A.Markulj Kulundžić,A.Josipović (2019): Isoflavone concentrations in soybeans suitable for growing in Europe.- Genetika, Vol 51, No.1, 47-59.

Isoflavones are nutraceuticals with many different medical benefits found abundantly in soybean (Glycine max (L.) Merr.) seeds. The prerequisite of utilising this valuable source of bioactive compounds and creating quality stock for the pharmaceutical and functional food industries is the screening of available soybean germplasm for isoflavone content. The objectives of this research were to determine the isoflavone concentrations (total isoflavones, daidzein, genistein, glycitein) in 22 high-yielding soybean genotypes, to investigate their variability and explore the effect of different weather conditions on isoflavone phenotypes. Field trials were set up as a randomised complete block design with two replicates in three consecutive years (2010 - 2012) at the Agricultural Institute Osijek (Osijek, Croatia). Chosen genotypes belonged to 00 - II maturity groups (MGs) suitable for growing in almost all European regions. Results showed the existence of genetic diversity among the tested plant material. The influence of genotype and year were both statistically significant. The divergence determined by the analysis of variance (ANOVA) and confirmed by the pair-wise similarity based on the Euclidean distance, confirmed that this set of genotypes was suitable for the use in future crossing programmes aiming to produce cultivars superior in isoflavone content in comparison to the existing ones.

Keywords: Glycine max, seed, isoflavones, environment

Corresponding author: Maja Matoša Kočar, Agricultural Institute Osijek, Južno predgrađe 17, Osijek, Croatia, E-mail: maja.matosa@poljinos.hr

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 61-68

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901061V

Original scientific paper

EFFECT OF THE SER638ARG VARIATION IN THE CAST GENE AND CAUSAL SNP G.1843C>T IN THE RYR1 GENE ON CARCASS TRAITS IN CROSBREED PIGS

Karel VEHOVSKÝ, Kateřina ZADINOVÁ ⃰, Roman STUPKA, Jaroslav ČÍTEK,

Monika OKROUHLÁ, Nicole LEBEDOVÁ, Michal ŠPRYSL

Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic

Abstract

Vehovský K., K. Zadinová, R. Stupka, J. Čítek, M. Okrouhlá, N. Lebedová, M. Šprysl (2019): Effect of the ser638arg variation in the CAST gene and causal SNP g.1843c>t in the RYR1 gene on carcass traits in crosbreed pigs.- Genetika, Vol 51, No.1, 61-68.

The objectives of this investigation was to demonstrate that genotypes of calpastatin (CAST) Ser368Arg and ryadonide receptor 1 (RYR1) g.1843C>T may affect the carcass traits of pigs. Association analysis of the aforementioned SNPs was performed on 518 pigs included eight commercial crossbreeds and one pure pig breed. All pigs were slaughtered at average body weight of 113kg. Genotypes at the SNPs were determined by PCR–RFLP. There were found only two genotypes of RYR1 gene: CC (428 pigs) and CT (90 pigs) in this study. The effect of allele C compared to allele T on the higher fat content of pig carcass was confirmed. The allelic frequencies of allele 638Ser (C) and allele 638Arg (A) were 0.26 and 0.74, respectively. The significant association (P<0.05) was occurred between allele A and higher fat content, and between C allele and higher lean meat content. Our results showed no significant effect of the observed polymorphismSer638Arg on the intramuscular fat content or other indicators of carcass value.

Keywords: Calpastatin, PCR-RFLP, meat quality, pig

Corresponding author: Kateřina Zadinová, Department of Animal Husbandry Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic, Tel: +420 224 383 051, E-mail: zadinova@af.czu.cz.

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 69-80

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901069S

Original scientific paper

COMPARATIVE AND PHYLOGENETIC ANALYSIS OF RUBISCO LARGE SUBUNIT (rbcL) PROTEINS IN SOME Sideritis L. (LAMIACEAE) SPECIES:

A BIOINFORMATIC APPROACH

Emre SEVİNDİK

Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey

Abstract

Sevindik E. (2019): Comparative and phylogenetic analysis of rubisco large subunit (rbcl) proteins in some Sideritis L. (Lamiaceae) species: a bioinformatic approach.- Genetika, Vol 51, No.1, 69-80.

The large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) protein, which plays an important role in the photosynthesis reaction, are encoded by the chloroplast genome. Sideritis L., a medical and aromatic plant group, belongs to Lamiaceae family. In this study, we performed sequence, physicochemical, phylogenetic and three-dimensional (3D) bioinformatic analyses of RuBisCO large subunit (rbcL) proteins in the Sideritis ssp. using various bioinformatics tools. Physicochemical analyzes were performed by ExPASy - ProtParam. The putative phosphorylation sites of the rbcL proteins were determined by NetPhos 2.0 and NetPhos 3.1. Phylogenetic analyses were performed with the MEGA 6.0 software. To estimate 3D protein structures, PyMol program was used. At the end of the study, it was found that the amino acid number of stilbene synthase proteins ranged between 171 and 456, molecular weight ranged between 19002.67 and 50420.44 Da, instability index ranged between 27.30 to 40.70 and GRAVY values ranged between -0.394 to -0.226. While the highest average amino acid rate in the rbcL proteins was Gly (10.00%), the lowest amino acid ratio (1.4%) was determined as Trp. In phylogenetic analyses performed using protein sequences, maximum likelihood (ML) tree consisted of 2 large clades. Pairwise distance analysis based on Sideritis species’ rbcL protein sequences was performed using MEGA 6.0. The lowest pairwise distance was 0.000, while the highest pairwise distance was 0.024. When the estimated 3D structures of the proteins were examined, the Gly residue, which plays an important role in the structure and function of the proteins, was detected as the least in S. sipylea species while it was the most abundant residue in S. cretica subsp. spicata. The results of our study provide insights into fundamental characteristics of rbcL proteins in Sideritis taxa.

Keywords: Sideritis, RuBisCO, rbcL, phylogenetic analysis, bioinformatic analysis

Corresponding author: Emre Sevindik, Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey, E-mail: ph.d-emre@hotmail.com

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Published in „GENETIKA“ Vol. 51, No.1(2019), pp.81-92

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1901081C

Original scientific paper

Selection of sunflower hybrids based on stability across environments

Sandra CVEJIĆ^1*, Siniša JOCIĆ¹, Velimir MLADENOV², Borivoje BANJAC², Ilija RADEKA¹, Milan JOCKOVIĆ¹, Ana JEROMELA MARJANOVIĆ¹, Dragana MILADINOVIĆ¹, Vladimir MIKLIČ¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

Abstract

Cvejić S., S. Jocić, V. Mladenov, B. Banjac, I. Radeka, M. Jocković, A. Jeromela Marjanović, D. Miladinović, V. Miklič (2019): Selection of sunflower hybrids based on stability across environments.- Genetika, Vol 51, No.1, 81-92.

Bearing in mind that the sunflower production in Serbia is extensive, it is very important to select a hybrid that will achieve stable and high seed and oil yields in different enviromental conditions. The objective of the study was to identify the most stable sunflower hybrids for seed and oil yield using AMMI model analysis. Nine newly developed early, medium early and medium late hybrids were tested. Hybrids were grown in different locations across Northern Serbia during two growing seasons (2013-2014). Selected locations are geographically close, but environmentally different in terms of soil, rainfalls and temperature, thus creating a specific microclimate conditions for sunflower growing. Pooled analysis of variance for seed and oil yield showed that the main effects of hybrids, environments and their interaction were highly significant. The agro-ecological environment E3 is most favourable for achieving high seed and oil yields, but E9 was the most stable. According to results of AMMI model, the most stable hybrids for the seed and oil yield, i.e. the lowest interaction with the enviroment, were NS2, NS8 and NS9. The obtained results indicate that hybrids can be recommended for production in different environments in Serbia; NS2 hybrid as early-growing, stable for oil yield; NS8 and NS9, medium-late hybrids, high productivity potential and suitable for all production systems.

Keywords: sunflower, hybrid, seed yield, oil yield, stability

Corresponding author: Sandra Cvejić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia. Phone: +381214898403; cell phone: +381648706028, e-mail: sandra.cvejic@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp.93-102

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1901093S

Original scientific paper

SEQUENCE ANALYSIS OF FLAVANONE 3-HYDROXYLASE AND DIHYDROFLAVONOL 4-REDUCTASE GENES IN WHEAT WITH NONSTANDARD COLOURED CARYOPSES

Klára ŠTIASNA, Mária PRESINSZKÁ, Tomáš VYHNÁNEK, Václav TROJAN,

Pavel HANÁČEK, Ladislav HAVEL

Department of Plant Biology, Mendel University in Brno, Faculty of Agronomy, Brno,

Czech Republic

Abstract

Klára Štiasna, Mária Presinszká, Tomáš Vyhnánek, Václav Trojan, Pavel Hanáček, Ladislav Havel (2019): Sequence Analysis of Flavanone 3-hydroxylase and Dihydroflavonol 4-reductase Genes in Wheat with Nonstandard Coloured Caryopses.- Genetika, Vol 51, No.1, 93-102.

The DNA sequences of chosen structural genes, flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR), encoding key enzymes from the flavonoid biosynthetic pathway, were studied in this paper. Sequences were gained using different approaches, i.e. direct sequencing from the PCR product for F3H and a cloning strategy for DFR. Five bread wheat (Triticum aestivum L.) genotypes with nonstandard coloured caryopses, purple, blue, and white, were used as plant material. The sequence variability was observed among tested genotypes.

Keywords: anthocyanins, DFR, F3H, flavonoids, Triticum aestivum L.

Corresponding author: Klára Štiasna, Department of Plant Biology, Mendel University in Brno, Faculty of Agronomy, Zemědělská 1, 613 00 Brno, Czech Republic, e-mail: klara.stiasna@mendelu.cz, phone: +420776769629

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 103-119

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901103A

Original scientific paper

CHROMOSOMAL EVOLUTION AND MOLECULAR GENETIC ANALYSIS OF FOUR SPECIES OF GENUS ANAS (AVES: ANATIDAE)

Ali Hussein ABU-ALMAATY^1*, Mohamed K. HASSAN¹, Neveen E. R. EL BAKARY²,

Sarah H. AHMED¹

¹Zoology Department, Faculty of Science, Port Said University, Egypt

²Zoology Department, Faculty of Science, Damietta University, Egypt

Abstract

Abu-AlmaatyA. H., M. K. Hassan, N. E. R. El Bakary, S. H. Ahmed (2019): Chromosomal evolution and molecular genetic analysis of four species of genus Anas (Aves: Anatidae).- Genetika, Vol 51, No.1, 103-119.

Birds are considered one of the least karyotypically examined animal groups due to their karyotype specificity, i.e. small chromosomes, a large diploid chromosome number and the separation of chromosomes into macro- and microchromosomes. The present work was aimed to investigate the number of chromosomes and their karyological and molecular genetic relationships of four species of genus Anas (Anas crecca, Anas penelope, Anas acuta and Anas clypeata (Family: Anatidae). All four species have the same diploid chromosome number of 2n=80. The four investigated species have shown five pairs of macrochromosomes and the remaining 35 pairs were of microchromosomes. Ten RAPD primers were used for molecular discrimination by polymerase chain reaction (PCR). The (PCR) showed polymorphic bands, which were used for the construction of the dendrogram and a similarity matrix. A total of 133 bands were obtained; 37 of them were polymorphic and 27 unique bands. Similarity values among the species under study ranged from 79% to 85%. The highest similarity was between A. Penelope and A. acuta (85%) while the lowest similarity was between A. acuta and A. clypeata (79 %). RAPD analysis confirmed that the four Anas species under study are genetically different from each other and a genetic variation was found between and within the three species tested in this study. The karyotypic features are also suitable as cytotaxonomic markers of Anatidae.

Keywords: Micro/Macro chromosomes, karyotype, RAPD, DNA, Anas, Anatidae.

Corresponding author: Ali Hussein Abu-Almaaty, PhD., Biotechnology Program, Department of Zoology, Faculty of Science, Port Said University, Port Said, 42521, Egypt.

E-mail: ali_zoology_2010@yahoo.com; Tel: 00201093041699

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 121-136

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901121B

Original scientific paper

THE PHENOTYPIC PLASTICITY OF of Picea omorika /Panč./Purkyne MORPHOLOGICAL POLLEN TRAITS

Branislava BATOS¹, Danijela MILJKOVIĆ^2*

¹Institute of Forestry, Department of Genetics, Belgrade, Serbia,

²`Siniša Stanković` Institute for Biological Research, Department of Evolutionary Biology,University of Belgrade, Belgrade, Serbia

Abstract

Batos B., D. Miljković (2019): The phenotypic plasticity of of Picea omorika /panč./purkyne morphological pollen traits.- Genetika, Vol 51, No.1, 121-136.

The variability of morphological traits of Picea omorika /Panč./Purkyne pollen was analyzed in two successive years on the pollen sampled from 24 trees in a seed stand at the site of Bela Zemlja in the area of Mt. Zlatibor (Serbia). The aim of the research was to obtain the index of phenotypic plasticity of the equatorial and polar axes and the coefficient of shape of pollen. According to the obtained results, Serbian spruce pollen grains are 93.3 µm/53.2 µm in size with their shape being oblate 57.5%. The climate characteristics of the study years initiated different responses of the pollen traits for each tree. Reaction norms were slightly steep and they crossed as confirmed by the significant interaction between the variability factors of the year and the tree. In the year with lower temperatures and less precipitation, the mean values of the equatorial axis were higher and the polar axis smaller, giving the pollen grain a more oblate form. According to the results of the applied model of analysis of variance where the year and the tree, as well as the interaction between them, were taken as factors of variability, the values of the pollen equatorial axis (length) and the coefficient of shape showed statistically significant differences between the years. Genetic variability (interindividual differences) for the values of both axes of pollen grains was confirmed by statistically significant differences between the trees in a single year. Interindividual differences in the observed morphological pollen traits were affected by environmental conditions specific to the year of sampling (statistically significant year x tree interaction). All the analyzed traits showed interindividual differences in the values of the plasticity index. The lowest values of plasticity were obtained for the equatorial axis (length) of the pollen grain, and they were significantly different from the plasticity indexes of the other traits analyzed herein. The plasticity of pollen traits has an important role in the controlled selection and breeding of species with the aim of obtaining more resistant genotypes with a greater ability to adapt to the fluctuations of environmental factors caused by global climate change.

Keywords: Picea omorika, pollen dimension, pollen shape, index of plasticity

Corresponding author: Danijela Miljković, PhD, (Orcid ID 0000-0003-1781-6658)

Department of Evolutionary Biology, `Siniša Stanković` Institute for Biological Research, Bulevar despota Stefana 142, 11060 Belgrade, Serbia, E-mail: danijela.miljkovic@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 137-146

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901137V

Original scientific paper

START CODON TARGETED POLYMORPHISM FOR EVALUATION OF FUNCTIONAL GENETIC VARIATION AND RELATIONSHIPS IN CULTIVATED CASTOR (Ricinus communis L.) GENOTYPES

Martin VIVODÍK*, Želmíra BALÁŽOVÁ, Zdenka GÁLOVÁ and Lenka PETROVIČOVÁ

Slovak University of Agriculture in Nitra, Nitra, Slovak Republik

Abstract

Vivodík M., Ž. Balážová, Z. Gálová, L. Petrovičová (2019): Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated castor (Ricinus communis L.) genotypes.- Genetika, Vol 51, No.1, 137-146.

In the present study, 111 castor genotypes were differentiated by the DNA fingerprinting patterns using 37 SCoT primers. The selected primers amplified DNA fragments across the 111 genotypes studied with the number of amplified fragments varying from 3 (SCoT 14) to 10 (SCoT 30 and SCoT 44) and the amplicon size varied from 100 to 3000 bp. Of the 246 amplified bands, 186 were polymorphic with an average of 5.03 fragments per primer. The percentage of polymorphic bands ranged from 57.14 % (SCoT 34) to 100.00 % (SCoT 28 and SCoT 33) with an average of 77.50%. The polymorphic information content (PIC) values varied from 0.372 (SCoT 14) to 0.818 (SCoT 30) with an average of 0.677. A dendrogram was constructed from a genetic distance matrix based on profiles of the 37 SCoT primers using the unweighted pair-group method with the arithmetic average (UPGMA). According to analysis, the collection of 111 diverse accessions of castor was clustered into two main clusters (1 and 2). The first cluster were subdivided into two subclusters (1a and 1b). Subclaster 1a contained 11 genotypes of castor and subclaster 1b contained 6 genotypes of castor. Subclaster 2 were subdivided into two subclusters (2a and 2b). Subclaster 2a contained 44 castor genotypes and subclaster 2b contained 50 castor genotypes. Results showed the utility of SCoT markers for estimation of genetic diversity of castor genotypes leading to genotype identification.

Keywords: ricin, SCoT markers, polymorphism, dendrogram, PIC.

Corresponding author: Martin Vivodík, Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Biochemistry and Biotechnology, Tr. A. Hlinku 2, Nitra, phone: +421376414269, e-mail: martin.vivodik@uniag.sk

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Published in „GENETIKA“ Vol. 51, No.1(2019), pp. 147-155

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901147D

Original scientific paper

STUDY OF GENETIC DIVERSITY OF IRANIAN INDIGENOUS BUFFALO POPULATIONS USING MICROSATELLITE MARKERS

Mahdiyeh YOUSEFI DARESTANI^*, Seyed-Resa MIRAEI ASHTIANI, Mostafa SADEGHI

Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Darestani Y. M., S.-R. M. Ashtiani, M. Sadeghi (2019): Study of genetic diversity of Iranian indigenous buffalo populations using microsatellite markers.- Genetika, Vol 51, No.1, 147-155.

Indigenous buffalo breeds represent a unique genetic resource, and understanding their variability, population structure and breeding units is important for their sustainable conservation. In the present study the genetic structure of Iranian buffalo populations were analyzed using ten microsatellite markers. Two hundred hair samples were collected and DNA was extracted using modified salting out method. After Polymerase Chain Reaction (PCR), the PCR products were electrophoresed using 9% polyacrylamide gel. Fifty nine alleles were observed for all the loci. The average number of alleles was 5.90 and the effective average number of alleles was 4.86. The high level of mean heterozygosity index between three populations indicate that the genetic diversity is high in within and between populations. The mean of polymorphism information content (PIC) value for all loci was 0.70. The FST value for the total loci was 0.01, indicating a very low level of genetic structure among populations. The genetic structure AMOVA analysis showed that about 3% of the total genetic variation was explained by population differences and 97 percent was corresponded to differences among individuals. The obtained results at the present study indicated that characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of buffalo genetic resources.

Keywords: Genetic Diversity, Iranian Buffalo, PCR, Microsatellite Markers, AMOVA analysis

Corresponding author: Mahdiyeh Yousefi Darestani, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran, m.yousefi.d@ut.ac.ir

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 157-166

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901157M

Original scientific paper

FETAL CHROMOSOMAL ANOMALIES IN SOUTHEAST SERBIA

- SINGLE CENTER COHORT RETROSPECTIVE STUDY

Radovan MILIĆEVIĆ¹, Ljiljana BRANKOVIĆ¹, Desanka RADULOVIĆ¹, Dragana JUGOVIĆ¹, Hristina STAMENKOVIĆ^1,3, Tatjana STANKOVIĆ^1,3, Aleksandar MILIĆEVIĆ², Višnja MADIĆ¹, Marina RISTIĆ¹

¹Pediatric Clinic, Clinical Center Niš, Niš, Serbia

²Center for Pathology and Pathological Anatomy, Clinical Center Niš, Niš, Serbia; ³University of Niš, Faculty of Medicine, Niš, Serbia

Abstract

Milićević R., Lj. Branković, D. Radulović, D. Jugović, H. Stamenković, T. Stanković, A. Milićević, V. Madić, M. Ristić (2019): Fetal chromosomal anomalies in southeast Serbia- single center cohort retrospective study.- Genetika, Vol 51, No.1, 157-166.

Congenital anomalies are the cause of prenatal death in 20-25% of the cases, while 3% of children are born with a malformation of varying size. Many of these anomalies can be detected before birth using different non-invasive and invasive prenatal diagnostic tests. This study was used to determine the distribution of genetic disorders in relation to the age of the mother, the frequency of aberrations and to study the effects and importance of prenatal diagnosis in South Serbia. Prenatal diagnostics was performed at the Pediatric Clinic within the Clinical Center of Niš. This retrospective study included a group of 8830 pregnant women, aged between 18 and 47 years during the period from 2004 to 2017. Amniocentesis was performed between the 16^th and 18^th week of pregnancy and involved the aspiration of 20 ml of amniotic fluid. Isolated cells were cultured in a medium that stimulates cell growth for 10 days. After cytogenetic processing, the obtained karyotype was analyzed using G-banding techniques. In 8830 samples of amniotic fluid cell cultures, 198 karyotypes with chromosomal aberrations were found - 179 with numerical aberrations and 19 with structural aberrations such as translocations, inversions and deletions. There were 85 karyotypes with autosomal numerical aberrations and 32 karyotypes with sex chromosome numerical aberrations. The most frequent one was trisomy 21 (106 cases). The highest number of autosomal numerical aberrations, 84%, was found in pregnancies where maternal age was above 30 years. Preventive action, advice, education and availability of prenatal diagnosis can lead to a significant reduction in the number of children born with various malformations.

Keywords: Genetic disorder, prenatal diagnostics, amniocentesis, cytogenetics, chromosomal aberration.

Corresponding author: Radovan Milićević, Pediatric Clinic, Clinical Center Niš, 48 Dr Zoran Đinđić Blvd, 18000 Niš, Serbia. Phone +381 63 7237163 E-mail: milickoyu@yahoo.com

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 167-178

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1901167M

Original scientific paper

GENETIC analysis of SPIKE length IN wheat

Velimir MLADENOV^*, Miodrag DIMITRIJEVIĆ¹,Sofija PETROVIĆ¹,

Jan BOĆANSKI¹, Borislav BANJAC¹, Ankica KONDIĆ-ŠPIKA², Dragana TRKULJA²

¹University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia.

²Institute for Field and Vegetable Crops, Novi Sad, Serbia.

Abstract

Mladenov V., M. Dimitrijević,S. Petrović, J. Boćanski, B. Banjac, A. Kondić-Špika, D. Trkulja (2019): Genetic analysis of spike length in wheat.- Genetika, Vol 51, No.1, 167-178.

The permanent need for efficient plant breeding comes from the increment of human population, which is projected to reach 9.7 million by 2050. Novel approaches could be used to reach these goals more rapidly, raising the question of efficiency, as well. Spike length is one of the important components of grain yield formation in wheat. The influence of individual plan traits is getting more important to grain yield formation per area unit in stressful growing conditions, which are increasingly present due to global climate changes. The objectives of the present research were three-fold: (i) to determine the influence of a genotype, environment and their interaction on spike length and to evaluate stability of the trait; (ii) to present cause-causing links on a graphical example; (iii) to transform a number of possibly correlated variables into a smaller number of uncorrelated variables called principal components. Samples were obtained from 96 winter wheat cultivars grown in 2011/12 and 2012/13 on two locations. The wheat genotype population was profiled with 28 microsatellites. The ANOVA of the total phenotypic variation of the experiment shows that genotypes took the largest portion, followed by the influence of the GE interaction. Additional analysis of the GE interaction using the PCA analysis shows a statistical significance of the first two main components. In the conducted research, the dispersion of the points represents two subpopulations, but the geographical origin could not explain the grouping of genotypes within the same, so the division into two groups was done on the basis of their lines of descent.

Keywords: AMMI, interaction, microsatellites, spike length, wheat.

Corresponding author: Velimir Mladenov; University of Novi Sad, Faculty of Agriculture Novi Sad, Sq. Dositeja Obradovića 8, 21 000 Novi Sad, phone: +381 (21) 485 32 36; e-mail: velimir.mladenov@polj.edu.rs

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 179-198

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901179W

Original scientific paper

Agro-morphological and chemical characterization

of traditional Indonesian peanut (Arachis hypogaea L.) cultivars

Sesilia Anita WANGET^1,2, María Remedios Morales-Corts¹, Rodrigo Pérez-Sánchez¹*, Neni ROSTINI³, María Ángeles Gómez-Sánchez¹, Agung KARUNIAWAN³

¹Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, Spain

²Faculty of Agriculture, Sam Ratulangi University, Manado, Indonesia

³Faculty of Agriculture, Padjadjaran University, Bandung, Indonesia

Abstract

Wanget S. A., M. R. Morales-Corts, R. Pérez-Sánchez, N. Rostini, M. Á. Gómez-Sánchez, A. Karuniawan (2019): Agro-morphological and chemical characterization of traditional Indonesian peanut (Arachis hypogaea L.) cultivars.- Genetika, Vol 51, No.1, 179-198.

In order to improve the knowledge of the peanut species, thirty five cultivars from several different regions in Indonesia were collected and characterized agro-morphologically and chemically. A total of 25 descriptors, mainly defined by the International Plant Genetic Resources Institute and the International Crops Research Institute for the Semi-Arid Tropics were used to describe the flowers, leaves and fruits obtained over 5 planting seasons (October 2010-February 2013). The study indicates that different growth seasons strongly affect the quantitative peanut parameters measured. A dendrogram, produced using the Furthest Neighbor Method (Euclidean) from agro-morphological and chemical parameters, afforded a clear separation between the peanut cultivars and revealed existing synonymies such as Gorontalo B and C and homonymies such as Kinali Merah and Putih. Some cultivars, such as Baturaja B, Rancabuaya, and Sumenep, exhibited an interesting combination of the high-yield (950 kg/ha), high content of omega-3 (3.42 g/100g) and omega-9 (38.11 g/100g) fatty acids, and resistance to Ralstonia solanacearum characters. Another two relevant cultivars related to chemical composition were Binjai and Sumedang. All these traditional cultivars can be used as a potential source of genetic variation for future peanut improvement programs. This work is an important step in the conservation of genetic peanut resources in Indonesia, which show distinctive and interesting agronomic and chemical characters, such as yield components, maturing time and oil composition.

Keywords: conservation, genetic resources, peanut descriptors, pod, unsaturated fatty acids

Corresponding author: Rodrigo Pérez-Sánchez, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Avda. Filiberto Villalobos, 119. 37007-Salamanca, Spain, Phone: 34-923-29-4690; Fax: 34-923-29-4774; E-mail: rodrigopere@usal.es

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 199-212

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901199T

Original scientific paper

HETEROSIS, COMBINING ABILITY AND GENE ACTION STUDIES IN CUCUMBER FOR DIFFERENT BIOTIC STRESSES TO DEVELOPE RESISTANT HYBRIDS

Manisha THAKUR^*, Ramesh KUMAR, Sandeep KANSAL

Department of Vegetable Science, Dr YS Parmar University of Horticulture and Forestry,

Nauni-173 230, Solan, HP, India

Abstract

Thakur M., R. Kumar, S. Kansal (2019): Heterosis, combining ability and gene action studies in cucumber for different biotic stresses to develope resistant hybrids.- Genetika, Vol 51, No.1, 199-212.

Biotic stress is the major constrains for the realization of crop yield potential. As climate change progresses, the spread and intensity of biotic stress is expected to increase, with increased probability of crops being exposed to stress. Shielding crops from stress requires a better understanding of the plant’s response and its genetic architecture. The dearth of research pertaining to the heterosis, combining ability and gene action studies for insect-pest (fruit fly) and disease incidences (powdery and downy mildew) in cucumber compels us to undertake this study. The experimental material comprised 15 F₁ crosses, developed by crossing 6 genotypes during the year 2015. Parents and the 15 hybrids, along with standard check (KH-1), were planted in a randomized complete block design during the year 2016 for screening against different insect-pest and diseases under natural field conditions. In the present studies, genotypes PI-618860, UHF-CUC-1, UHF-CUC-2 and Khira-75 and crosses Khira-75 x PI-618860, Khira-75 x UHF-CUC-1 and Khira-75 x UHF-CUC-2 were found superior in response to insect-pest and disease incidences. Further, gene action studies indicated predominant role of non-additive gene action governing all the traits under study.

Keywords: Biotic stress, cucumber, fruit fly incidence, powdery mildew and downy mildew.

Corresponding author: Manisha Thakur, Department of Vegetable Science, Dr YS Parmar University of Horticulture and Forestry, Nauni-173 230, Solan, HP, IndiaE-mail: mnshthakur4@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp.213-226

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901213E

Original scientific paper

NUCLEOTIDE DIVERSITY OF Cyt b GENE IN Drosophila subobscura Collin

Pavle ERIĆ^1*, Mihailo JELIĆ², Marija SAVIĆ VESELINOVIĆ², Bojan KENIG¹,

Marko ANĐELKOVIĆ^1,2,3, Marina STAMENKOVIĆ-RADAK^1,2

¹Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

² Faculty of Biology, University of Belgrade, Belgrade

³ Serbian Academy of Sciences and Arts, Belgrade

Abstract

Erić P., M. Jelić, M. Savić Veselinović, B. Kenig, M. Anđelković, M. Stamenković-Radak(2019): Nucleotide diversity of Cyt b gene in Drosophila subobscura Collin.- Genetika, Vol 51, No.1, 213-226.

Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host’s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size.

Keywords: mtDNA, haplotype network, singleton, fruit fly, population history

Corresponding author: Pavle Erić, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despot Stefan blvd. 142, 11000 Belgrade, Serbia, pavle.eric@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 227-236

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901227A

Original scientific paper

GENETIC DIVERSITY IN Cola acuminata AND Cola nitida USING RAPD PRIMERS

Lawrence A. AKINRO*, Adenubi I. ADESOYE, Taiye R. FASOLA

Department of Botany, University of Ibadan, Ibadan, Nigeria

Abstract

Akinro A. Lawrence, A. I. Adesoye, T. R. Fasola (2019): Genetic diversity in Cola acuminata and Cola nitida using RAPD primers.- Genetika, Vol 51, No.1, 227-236.

Cola species constitute an important non–timber forest product. Besides the food value, Cola is rich in numerous phytochemicals, making it more important for its use in both African traditional medicine and potentials in industrial pharmacopoeia. Knowledge about genetic diversity is essential for conservation. In this paper, we reported genetic variability of Cola acuminata and C. nitida germplasm across the Cola – producing states (the rain forest and derived savannah zones) in Nigeria using Random Amplified Polymorphic DNA (RAPD) markers. Fifteen primers which gives an average of 6.5 bands per primer were selected for both species. C. acuminata exhibited a higher level of variation with 71.5% of the detected markers being polymorphic (223 polymorphic alleles), whereas C. nitida presented 58.3% variation with 182 polymorphicalleles. Inter-population differentiation was measured as Jaccard’s similarity coefficient. The mean similarity index amounted to 42.5% in C. acuminata and 46.7% in C. nitida respectively. Results reveal the genetic structure of both species and conservation strategies are suggested.

Keywords: Cola, conservation, biodiversity, forest resources, random amplified polymorphic DNA (RAPD), molecular markers.

Corresponding author: Lawrence Abiola Akinro, Department of Botany, Faculty of Science, University of Ibadan, Ibadan, Nigeria. e-mail: lawrenceakinro@gmail.com, Phone: +2348034978114

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 237-249

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1901237N

Original scientific paper

DIVERGENCE AMONG MAIZE GENOTYPES WITH DIFFERENT KERNEL TYPES ACCORDING TO SSR MARKER ANALYSIS

Ana NIKOLIĆ^*, Natalija KRAVIĆ, Jelena SRDIĆ, Dragan KOVAČEVIĆ,

Violeta ANĐELKOVIĆ, Milomir FILIPOVIĆ, Snežana MLADENOVIĆ DRINIĆ

Maize Research Institute “Zemun Polje”, Belgrade, Serbia

Abstract

Nikolić A., N. Kravić, J. Srdić, D. Kovačević, V. Anđelković, M. Filipović, S. Mladenović Drinić (2019): Divergence among maize genotypes with different kernel types according to SSR marker analysis.- Genetika, Vol 51, No.1, 237-249.

Panels of diverse materials have proven to be very useful in evaluation of the organization of genetic diversity available for breeding at phenotypic and genotypic levels. In this study, a panel consisting of several groups of maize inbreds was tested using microstelite markers. The aim of this study was to test genetic diversity and define population structure of twenty-four genotypes differing in kernel type (dent, flint, popcorn, sweet maize) and kernel color (white, yellow, orange) with 21 SSRs. Genetic diversity parameters such as number of alleles, gene diversity, observed heterozygosity, PIC (Polymorphism Information Content) and number of unique alleles were determined. In addition, genetic distances according to Rogers distance were calculated. The values for all parameters were high, reflecting high genetic divergence of analyzed germplasm. Distance matrix based on UPGMA cluster analysis showed moderate congruence to available pedigree/origin or kernel type/color data. Genetic structure of tested genotypes was defined using Bayesian model-based clustering, without a considerable difference in comparison to cluster data analysis. The determined diversity parameters along with the results concerning genetic structure analysis provided a valuable information for improved selection efficiency.

Keywords: inbred line, kernel type/color, microsatellites, population structure, Zea mays L.

Corresponding author: Ana Nikolić, Maize Research Institute, E-mail: anikolic@mrizp.rs; Phone: + 381 64 840 6216

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 251-260

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901251Y

Original scientific paper

PRELIMINARY FINDINGS OF CRYPTIC DIVERSITY OF THE GIANT TIGER SHRIMP (Penaeus monodon Fabricius, 1798) IN INDONESIA INFERRED FROM COI MITOCHONDRIAL DNA

Amri YUDHISTIRA^1,2 and dan Tuty ARISURYANTI^1*

¹Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Tehnika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia

²Central Brackishwater Aquaculture Fishery (BBPBAP) Jepara, Jl. Cik Lanang Bulu, Jepara 59418, Central Java, Indonesia

Abstract

Yudhistira A. and dan T. Arisuryanti (2019): Preliminary findings of cryptic diversity of the giant tiger shrimp (Penaeus monodon Fabricius, 1798) in Indonesia inferred from COI mitochondrial DNA.- Genetika, Vol 51, No.1, 251-260.

This study investigated genetic diversity of the giant tiger shrimp (Penaeus monodon), an economically important penaeid species in Indonesia, using 558 base pairs of the mitochondrial cytochrome c oxidase Subunit I (COI) gene. A total of 10 samples were collected from three populations throughout Indonesia and three samples were taken from hatchery. The mitochondrial COI results found high levels of genetic differentiation. From Bayesian tree building method there were two clades on phylogenetic tree with high posterior probability value 1.00. COI mt-DNA analyses revealed that there were six haplotypes in which four haplotypes in clade A and two haplotypes in clade B. Divergences of COI between two clades showed an average 6.9% (range=6.7%-7.6%), while genetic distance within clade A 0.2% (range=0-0.5%) and within clade B 0.3% (range=0-1.1%). Results from this study suggest the occurrence of two cryptic species in the Penaeus monodon from Indonesia.

Keyword: Penaeus monodon, cryptic species, genetic diversity, COI.

Corresponding author: Tuty Arisuryanti, Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Tehnika Selatan Sekip Utara, Yogyakarta 55281, Indonesia. E-mail : tuty-arisuryanti@ugm.ac.id

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 261-274

Serbia

UDC 575

https://doi.org/10.2298/GENSR1901261Z

Original scientific paper

EXPRESSION AND FUNCTION ANALYSIS OF WHEAT EXPASIN GENES EXPA2 AND EXPB1

Dong ZHU^1,4, Yanlin LIU^1,4, Man JIN¹, Guanxing CHEN¹,

Slaven PRODANOVIC^3*, Yueming YAN^1,2*

¹College of Life Science, Capital Normal University, 100048 Beijing, China

²Hubei Collaborative Innovation Center for Grain Industry (HCICGI), Yangtze University, 434025 Jingzhou, China

³Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

⁴Contributed equally to this work

Abstract

Zhu D., Y.Liu, M.Jin, G.Chen, S. Prodanovic, Y.Yan (2019): Expression and function analysis of wheat expasin genes EXPA2 and EXPB1.- Genetika, Vol 51, No.1, 261-274.

Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of α-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the β-expansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.

Keywords: expansins, expression, function, TaEXPA2, TaEXPB1, wheat

Corresponding author: Slaven Prodanovic, Faculty of Agriculture, University of Belgrade, 11080 Belgrade, Serbia, phone/fax: +86-10-82105173, E-mail: slavenp@agrif.bg.ac.rs

Yueming Yan, College of Life Science, Capital Normal University, Beijing, XisanhuanBeilu 105, 100048 Beijing, P.R. China, phone/fax: +86-10-68902777, E-mail: yanym@cnu.edu.cn

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp.275-285

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1901275K

Original scientific paper

Comparative biological characteristic of white lupin

(Lupinus albus L.) varieties

Valentin KOSEV and Viliana VASILEVA

Institute of Forage Crops, Pleven, Bulgaria

Abstract

Kosev V. and V. Vasileva (2019): Comparative biological characteristic of white lupin (Lupinus albus L.) varieties.- Genetika, Vol 51, No.1, 275-285.

The study was conducted in 2014-2016 on the experimental field of the Institute of Forage Crops, Pleven, Bulgaria. Aboveground and root biomass plant material of seven white lupin (Lupinus albus L.) varieties different originated was analyzed in two phenological stages. Plants were analyzed for height, fresh weight, number of leaves, nodule number and nodule weight in the beginning of flowering stage, and for number of pods, number of seeds and seed weight in the technical maturity stage. Degree of earliness of varieties was assessing as well. The group of ultra early varieties can be defined PI533704 and Zuter varieties with coefficient of earliness 1.00, to early - PI368911, PI457938 and KALI (coefficient of earliness 1.25), and to late Lucky801 and PI457923 (coefficient of earliness >1.66). A strong positive correlation was found between the seed productivity with number of seeds per plant (r=0.943) and plant height (r=0.765); close relationship of fresh aboveground mass weight with plant height (r=0.822), number of leaves (r=0.965) and fresh root mass weight (r=0.876). The varieties of interest for breeding were selected by different signs. It was concluded that the number and weight of nodules as well fresh root mass weight can be used as selection criteria for creating varieties with a higher symbiotic nitrogen fixation potential.

Keywords: earliness, productivity, root mass, white lupin

Corresponding author: Viliana Vasileva, Institute of Forage Crops, 89 “General Vladimir Vazov” Str., 5800 Pleven, Bulgaria, E-mail: viliana.vasileva@gmail.com, phone: + 359 886 06 54 61

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Published in „GENETIKA“ Vol. 51, No. 1(2019), pp. 287-298

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1901287B

Original scientific paper

Studies on gene flow from herbicide resistant to weedy sunflower

Dragana BOŽIĆ¹, Markola SAULIĆ¹, Aleksandra SAVIĆ^1,2, George GIBBINGS³,

Sava VRBNIČANIN¹*

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²Institut for Plant Protection and Environment, Belgrade, Serbia

³School of Agriculture, Policy and Development, University of Reading, Reading RG6 6AR, UK

Abstract

Božić D., M. Saulić, A. Savić, G. Gibbings, S. Vrbničanin (2019): Studies on gene flow from herbicide resistant to weedy sunflower.- Genetika, Vol 51, No.1, 287-298.

Gene flow is a main concern associated with the use of herbicide resistant sunflower crops because it could transfer herbicide resistance traits to weedy sunflower. In order to estimate potential gene flow from imazamox and tribenuron-methyl resistant sunflower hybrids to weedy sunflower, field experiments and DNA analysis were conducted. The progeny of weedy sunflower which grown near imazamox (WS1) and tribenuron-methyl (WS2) resistant hybrid in previous experiments were used. In the field experiment, recommended rates of imazamox and tribenuron-methyl were applied to WS1 and WS2, respectively, and plants surviving were recorded. Herbicides effect on fresh weight of survived plants were also determined. The presence of mutations responsible for sunflower resistance to herbicides (imazamox and tribenuron-methyl) checked based on DNA analysis of selected survived plants. Percentage of survived plants in field experiment was recorded at maturity and depend on weedy sunflower accession (WS1or WS2) and distance of their mother plants from resistant sunflower hybrid in previous experiment and was higher for WS2 (50.25%) than for WS1 (24.50%). As DNA analysis were not confirmed the presence of the point mutations responsible for sunflower resistance to imazamox and tribenuron-methyl.

Keywords: DNA analysis, gene flow, HR sunflower, weedy sunflower

Corresponding author: Sava Vrbničanin, University of Belgrade, Faculty of Agriculture, Namanjina 6, Belgrade, Serbia, E-mail: sava@agrif.bg.ac.rs, Tel:+381114413307

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp 299-312

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901299A

Original scientific paper

EVALUATION OF PROTECTIVE EFFECTS OF PROPOLIS AGAINST ALUMINIUM SILICATE TOXICITY IN RATS

Yasmin M. Abd EL-AZIZ¹, Ali H. ABU-ALMAATY¹, Nahed A.OMAR², Ahmed M. ABDEEN³, Mahmmoud M. ZAKARIA⁴*

¹Zoology Department, Faculty of Science, Port Said University, Egypt

²Zoology Department, Faculty of Science, Damietta University, Egypt

³Zoology Department, Faculty of Science, Mansoura University, Egypt

⁴Urology & Nephrology Center, Faculty of Medicine, Mansoura University, Egypt

Abstract

Abd El-Aziz Y. M., A.H. Abu-Almaaty, N. A.Omar, A. M. Abdeen, M. M. Zakaria, (2019): Evaluation of protective effects of propolis against aluminium silicate toxicity in rats.- Genetika, Vol 51, No.1, 299-312.

Objective of the study was evaluation of the harmful effect induced by aluminum silicate in bone marrow chromosomes and liver tissues. Also, how could prevents this toxicity. 60 Adult male albino rats weighing 100-120 g were used for our experiments. The animals were divided into six equal groups: first normal healthy control group, second group was given 200 ml propolis/Kg day after day by stomach gavage, third group received low dose of aluminum silicate (5 mg/kg; intra peritoneal) twice a week, forth group injected with high dose of aluminum silicate (20 mg/kg; intra peritoneal) twice a week, fifth group received propolis and aluminum silicate with similar doses as that of second & third groups and sixth group received propolis and aluminum silicate with similar doses as that of second & forth groups. At the end of 8 weeks for each rat, bone marrow was aspirated and liver was removed for lab examinations. The results showed that rats exposed to aluminum silicate had severe chromosomal aberrations and changes in CYP gene expression. It caused significantly increased in the frequency of chromosomal aberrations such as polyploidy, hypoploidy, deletions, fragments, chromatid breaks, chromosome breaks, double minutes, ring, gap and translocation were also observed. Gene expression of CYPs was increased with exposure to aluminum silicate and was down regulated in treatment with propolis while Bcl-2 and BAX were decreased in exposure to aluminum silicate and up regulated by the treatment. Propolis has a curative effect against aluminum silicate toxicity owing to its antioxidant property.

Keywords: aluminum silicate, chromosome aberrations, gene expression, propolis.

Corresponding author: Mahmmoud M. Zakaria, Biotechnology Department, Urology & Nephrology Center, Faculty of Medicine, Mansoura University, Egypt. P.O. Box: 35516, E-mail: mahmoudzakaria2004@yahoo.com

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 313-322

UDC 575.633

https://doi.org/10.2298/GENSR1901313G

Original scientific paper

AN ALTERNATIVE STATISTICAL MODEL FOR THE ASSESSMENT OF DRY MATTER ACCUMULATION IN COOL SEASON CEREALS: COX REGRESSION

Zeynep GÖKKUŞ^1* and Mevlüt AKÇURA²

¹Kastamonu University, İnebolu Vocational School, Department of Computer Technologies, Kastamonu, Turkey

²Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Canakkale, Turkey

Abstract

Gökkuş Z. and M. Akçura (2019): An alternative statistical model for the assessment of dry matter accumulation in cool season cereals: Cox regression.- Genetika, Vol 51, No.1, 313-322.

In this study, the growing behaviors of some cool season cereals (bread wheat, rye, durum wheat and barley) cereals were modeled simultaneously during the two growing seasons. For this purpose, Cox Regression was proposed as an alternative to the preferred regression methods in previous studies. In the study, based on the seasonal data of two different season growing seasons (2012-2013, 2013-2014 and both), each of which has 5 replicates 27 samples, growth rates of these cereals via dry matter accumulation quantities were explained in three different models. For this purpose, the dry matter accumulation amounts were fitted to the survival data and Cox Regression method, which uses the hazard function, the rate of occurrence of a particular event, was preferred. As a result, each model was found to be very important (p <0.000). It was determined that i) the fastest growing species was barley, ii) dry matter accumulation decreased as temperature increased, and iii) dry matter accumulation in crops changed during each growth season.

Keywords: Cox regression model, cool season cereals, growing model

Corresponding author: Zeynep Gökkuş, Kastamonu University, İnebolu Vocational School, Department of Computer Sciences, Kastamonu, Turkey, email:z.gokkus@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 1 (2019), pp. 323-334

Serbia

UDC 575.633

https://doi.org/10.2298/GENSR1901323B

Original scientific paper

QUANTITATIVE TRAIT LOCI DETECTION IN BULGARIAN COTTON MUTANT SEGREGATING POPULATION

B. M. BOJINOV

Department of Genetics and Plant breeding, Faculty of Agronomy, Agricultural University of Plovdiv, Bulgaria

Abstract

Bojinov M.B. (2019): Quantitative trait loci detection in Bulgarian cotton mutant segregating population.- Genetika, Vol 51, No.1, 323-334.

Identification of quantitative trait loci (QTL) linked to fiber quality is of primary interest in cotton breeding. As Bulgarian cotton varieties belong to a specific group (proles Bulgaricum) they are even more difficult for cross-breeding into them such quality characteristics. Therefore a segregating mutant population has been developed from the standard Bulgarian variety ‘Chirpan 603’ by applying seed gamma irradiation. M4 and M5 generations were used for identification of QTLs related to fiber quality characteristics. SSR markers developed in interspecific crosses and further confirmed in intraspecific crosses, together with in-house developed ISSR markers were used for association mapping of QTLs for fiber quality. Fiber strength, length, uniformity, micronaire and elongation were the main studied characteristics. QTLs with major effects on these traits identified in M4 and M5 were confirmed in M6 generation. Further ones were identified and used for map saturation and linkage group confirmation.

Keywords: Cotton, Gossypium hirsutum, mutation, mapping, QTL.

Corresponding author: B. M. Bojinov, Department of Genetics and Plant bbreeding, Faculty of Agronomy, Agricultural University of Plovdiv, 12 Mendeleev str., 4000 Plovdiv, Bulgaria. E-mail: bojinov@au-plovdiv.bg. Phone: +359 898 229 328

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Published in „GENETIKA“ Vol. 51, No. 1(2019), pp. 335-345

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901335R

Original scientific paper

GENETIC DIVERSITY AND STRUCTURE OF AUTOCHTHONOUS CATTLE BREEDS FROM BOSNIA AND HERZEGOVINA BASED ON MICROSATELLITES

Biljana ROGIĆ¹, Božo VAŽIĆ¹, Mihajla ĐAN², Marina STAMENKOVIĆ RADAK³

¹University of Banja Luka,Faculty of Agriculture, Banja Luka, Republic of Srpska, B&H

²University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Novi Sad, Serbia

³University of Belgrade, Faculty of Biology, Belgrade, Serbia

Abstract

Rogić B., B. Važić, M. Đan, M. Stamenković Radak (2019): Genetic diversity and structure of autochthonous cattle breeds from Bosnia and Herzegovina based on microsatellites.- Genetika, Vol 51, No.1, 335-345.

Genotype data from 21 microsatellites were used to evaluate genetic diversity and relationships of Gatacko cattle and Busha breeds. A total of 100 animals included in the study were divided into three groups: Gatacko cattle (GC), Busha from eastern Herzegovina (BEH) and Busha from western Herzegovina (BWH). Hardy-Weinberg equilibrium was observed for all loci in all groups, except for single locus (INRA35) in group of Busha from western Herzegovina. A total number of 166 alleles were detected in a total sample. The average number of alleles per locus was 7.9. The highest observed mean heterozygosity (0.688) was found in the Gatacko cattle and the lowest (0.634) in Busha from western Herzegovina. A pairwise population matrix of F_stvalues among analyzed groups showed significantly high genetic differentiation among all three groups: Gatacko cattle and Busha from western Herzegovina, Gatacko cattle and Busha from eastern Herzegovina and Busha from western and Busha from eastern Herzegovina. This report represents the first genetic characterization of Gatacko cattle and Busha cattle, traditional cattle breed from Bosnia and Herzegovina. The obtained results are valuable for the future development of conservation and management strategies for these cattle breeds.

Keywords: genetic diversity and structure, microsatellites, Gatacko cattle, Busha

Corresponding author: Biljana Rogić, University of Banja Luka,Faculty of Agriculture, Bulevar vojvode Petra Bojivića 1a, 78000 Banja Luka, Republic of Srpska, B&H

biljana.rogic@agro.unibl.org

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Published in „GENETIKA“ Vol. 51 No. 1 (2019), pp. 347-355

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901347G

Review paper

DRUG REPOSITIONING FOR A RARE GENETIC DISORDER PROGRESSIVE

Osseous heteroplasia (POH)

Jelena GVOZDENOVIĆ-JEREMIĆ^1* and Ljiljana MOJOVIĆ²

¹Nostopharma, LLC, Bethesda, MD, USA

² University of Belgrade, Faculty of Technology and Metallurgy, Belgrade, Serbia

Abstract

Gvozdenović-Jeremić J. and Lj. Mojović (2019): Drug repositioning for a rare genetic disorder progressive Osseous heteroplasia (POH).- Genetika, Vol 51, No.1, 347-355.

Progressive osseous heteroplasia (POH) is an ultrarare genetic disease of progressive ectopic ossification caused by heterozygous inactivating mutations of GNAS, the gene encoding the alpha subunit of the G-stimulatory protein of adenylyl cyclase (Gsα). Extensive ossification of the deep connective tissues can result in ankylosis of affected joints and growth retardation of involved limbs. Inhibition of main molecular signaling, Hedgehog (Hh) pathway, by pharmacological methods may reduce the severity of ectopic bone formation in POH patients. Hh inhibitors currently used or known for other conditions may be potential candidate drugs for treating this debilitating disease. In this study, three potential Hedgehog pathway inhibitors such as arsenic trioxide, statin, and vitamin D and their combinations were tested on subcutaneous mesenchymal progenitor (SMP) cells of Gαs f/f mice model for possible therapeutic application for POH. The combination of these three drugs at their significantly reduced concentrations retained anti-osteogenic activity in SMP cells with aberrant Hedgehog activity. In that light, we propose here a potential new approach of the drug combination in order to reduce potential toxicity, the side effect and increase success rate for Hh inhibitors drug repositioning.

Keywords: drug repurposing, ectopic ossification, Hedgehog pathway inhibitors, mesenchymal progenitor cells, Progressive Osseous Heteroplasia

Corresponding author: Jelena Gvozdenović-Jeremić, Nostopharma, LLC, Bethesda, MD, USA,

jelenaj@nostopharma.com; phone no. +1 703 679 0048

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 357-363

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1901357N

Orginal scientific paper

Maize resistance to ear rot caused by aspergillus parasiticus

Milica NIKOLIĆ, Iva SAVIĆ, Ana NIKOLIĆ, Goran STANKOVIĆ, Nenad DELIĆ,

Snežana MLADENOVIĆ DRINIĆ, Slavica STANKOVIĆ

Maize Research Institute, Zemun Polje, Belgrade, Serbia

Abstract

Nikolić M., I. Savić, A. Nikolić, G. Stanković, N. Delić, S. Mladenović Drinić, S. Stanković (2019): Maize resistance to ear rot caused by Aspergillus parasiticus.- Genetika, Vol 51, No.1, 357-363.

The fungus Aspergillus parasiticus produces aflatoxins, the most important group of mycotoxins considering their potential toxicity that may cause cancer in humans. Prevention is the most important and economically most beneficial practice in the reduction of fungal growth and mycotoxin production. Due to that, the development of resistant maize genotypes is the most effective method. The aim of the present study was to analyse the resistance of maize hybrids to pathogenic and toxigenic A. parasiticus isolates originating from maize in Serbia. Hybrids used belong to three FAO maturity groups and showed a high level of resistance to A. parasiticus species. A combination of prevention management strategies and good grain management at harvest can lessen the impact of Aspergillus ear rot on yield and grain quality.

Keywords: tolerance, genotypes, A. parasiticus

Corresponding author: Milica Nikolić, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185, Belgrade, Serbia, E-mail: mnikolic@mrizp.rs

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Published in „GENETIKA“ Vol. 51, No.1 (2019), pp. 365

Serbia

IN MEMORIAM

Academician Ljubomir Berberović, one of bards of Yugoslav genetics, passed away in 86^th year of his life

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