GENETIKA, Vol

GENETIKA, Vol. 51, No. 2 (2019)

Meysam LATIFI and Ali MOHAMMADI

ESTIMATION OF GENETIC PARAMETERS OF AUTOSOMAL AND SEX-LINKED PRE-WEANING TRAITS IN MAKUIE SHEEP USING MULTIVARIATE ANALYSIS [Abstract] [Full text]

Milka BRDAR-JOKANOVIĆ, Anamarija KOREN, Branka LJEVNAIĆ-MAŠIĆ, Biljana KIPROVSKI, Vladimir SIKORA

YIELD AND QUALITY PARAMETERS OF HOKKAIDO TYPE PUMPKINS GROWN IN SERBIA [Abstract] [Full text]

Beatriz GarcIa-GÓmez, Heidy GonzÁlez-ALVAREZ, Celia MartÍnez-Mora, José Luis Cenis, María del Carmen Pérez-Hernández, Yamila Martínez-Zubiaur, Pedro MartÍnez-GÓmez

THE molecular characterization of AN EXTENDED mulberry germplasm BY SSR markers [Abstract] [Full text] [Supplement]

Kalidas PATI, Anilabh Das MUNSHI, Manjusha VERMA, Tusar Kanti BEHERA, Lalit ARYA

GENETIC DIVERSITY OF CUCUMBER GENOTYPES REVEALED BY SSR MARKERS AND AGRONOMIC TRAITS [Abstract] [Full text]

Slavka KALAPCHIEVA, Elena TOPALOVA, Valentina PETKOVA

MORPHOLOGICAL, PHYSIOLOGICAL AND PRODUCTIVITY RESPONSE IN GARDEN PEA GENOTYPES DURING HIGH TEMPERATURE STRESS [Abstract] [Full text]

Esmaeil GHOLINEZHADand Reza DARVISHZADEH

PATH ANALYSIS FOR SEED YIELD IN SESAME (Sesamum indicum L.) INOCULATED/NON-INOCULATED WITH MYCORRHIZAL FUNGI UNDER DROUGHT STRESS [Abstract] [Full text] [Supplement]

Imren KUTLU, Zeynep SIREL, Ozcan YORGANCILAR, Aysel YORGANCILAR

LINE × TESTER ANALYSES FOR ANTHER CULTURE RESPONSE OF BREAD WHEAT (Triticum aestivum L) [Abstract] [Full text]

Iva PRUNER, Branko TOMIC, Marija DRAGOJEVIC, Maja GVOZDENOV, Mirjana KOVAC, Dragica RADOJKOVIC, Valentina DJORDJEVIC

INHERITED THROMBOPHILIC RISK FACTORS IN SERBIAN BREAST CANCER PATIENTS [Abstract] [Full text]

Masoumeh BAGHERI and Azadeh ZAHMATKESH

ASSESSMENT OF GENETIC DIVERSITY OF Asparagus racemosus Willd. FROM DIFFERENT AGRO-ECOLOGICAL ZONES OF KERALA USING RAPD MARKERS [Abstract] [Full text]

Chithra Madhanamohanan GEETHA and Elenjikkal Avarachan SIRIL
ASSOCIATION OF SLC11A1 GENE AND PRODUCTION TRAITS USING LOGISTIC REGRESSION [Abstract] [Full text]

Biljana KIPROVSKI, Jegor MILADINOVIĆ, Anamarija KOREN, Đorđe MALENČIĆ,Maja MIKULIČ-PETKOVŠEK
BLACK AND YELLOW SOYBEAN: CONTRIBUTION OF SEED QUALITY TO OXIDATIVE STRESS RESPONSE DURING PLANT DEVELOPMENT [Abstract] [Full text]

Dilyara GRITSENKO, Aleksandr POZHARSKY, Nina DERYABINA, Aliya KASSENOVA, Nurbol GALIAKPAROV
GENETIC ANALYSIS OF HEMAGGLUTININ PROTEINS OF H3 AND H1 SUBTYPES IN KAZAKHSTAN [Abstract] [Full text]

Hasan KOÇ
RELATIONSHIPS BETWEEN SURVIVAL IN WINTER COLDS AND SOME MORPHOLOGICAL AND TECHNOLOGICAL CHARACTERISTICS IN SAFFLOWER GENOTYPES [Abstract] [Full text]

Malik ASHIQ RABBANI, Sohail AHMAD JAN, Sadar UDDIN SIDDIQUI, Abdul GHAFOOR and Zahoor AHMAD
MORPHO-BIOCHEMICAL CHRACTERIZATION OF AMLA (Phyllanthus emblica L.) AND TAMARIND (Tamarindus indica L.) GERMPLASM FROM PAKISTAN [Abstract] [Full text]

Tejpal Singh SRAN and S. K. JINDAL
MULTI-ENVIRONMENTAL EVALUATION OF CHILLI GENOTYPES FOR YIELD AND YIELD COMPONENTS [Abstract] [Full text]

Valentin KOSEV and Viliana VASILEVA
Genetic analysis of quantitative TRAITS of grass pea (Lathyrus sativus L.) genotypes [Abstract] [Full text]

Mahmut Kaplan, Veysel Turan, Yusuf Murat KARDES, Aydin DAS, Kagan KOKTEN
FATTY ACID AND TRACE ELEMENT COMPOSITIONS OF THE SEEDS OF DIFFERENT Onobrychis viciifolia GENOTYPES [Abstract] [Full text]

Mahdi BADRI ANARJAN, Farshid TALAT, and Amir FAYYAZ MOGHADDAM
YIELD COMPONENTS ANALYSES IN COTTON: G. hirsutum CULTIVARS WITH MULTIVARIATE STATISTICAL METHODS [Abstract] [Full text]

Dušica ADEMOVIĆ-SAZDANIĆ andSvetlana VOJVODIĆ
HUMAN LEUKOCYTE ANTIGEN POLYMORPHISMS AS SUSCEPTIBILITY RISK FACTORS FOR END STAGE RENAL DISEASE [Abstract] [Full text]

Dunja Rukavina, Amir Zahirović, Ćazim CRNKIĆ, Mirela Mačkić-Đurović, Adaleta Durmić-Pašić, Belma Kalamujić Stroil, Naris Pojskić
USEFULNESS OF THE 17-PLEX STR KIT FOR BOSNIAN MOUNTAIN HORSE GENOTYPING [Abstract] [Full text]

A.Nilgun ATAY, Ersin ATAY, Burak KUNTER, K. Yaprak KANTOGLU, Nejdet KAPLAN
DETERMINATION OF OPTIMAL MUTAGEN DOSAGE AND ITS EFFECTS ON MORPHO-AGRONOMIC TRAITS IN PUTATIVE MUTANTS OF ‘AMASYA’ APPLE [Abstract] [Full text]

selvakumar, R., Pritam kalia, R.S., raje
GENETIC ANALYSIS OF NUTRITIONAL TRAITS IN TROPICAL CARROT (Daucus carota L.) [Abstract] [Full text]

Farah FARAHANI, Atena BAYANI, Zahra NOORMOHAMMADI
ASSESSMENT OF GENETIC DIVERSITY AMONG TWO in vitro Aole barbadensis AND Aloe littoralis REGENERATED PLANTS USING C-VALUE DNA OF FLOW CYTOMETRY [Abstract] [Full text]

Vladislava GALOVIC, Milan DREKIĆ, Sreten VASIĆ, Siniša ANDRAŠEV, Saša PEKEČ, Dejan V.STOJANOVIĆ, Verica VASIĆ
MITOCHONDRIAL 16S rDNA PROFILING AND PHYLOGENETIC ANALYSIS SUGGEST GENETIC DIVERSITY OF ASH WEEVIL (Stereonichus fraxini De Geer) IN SERBIA[Abstract] [Full text]

Ali Akbar EBADI, Mohammad Taher HALLAJIAN, Mojtaba KORDROSTAMI
The genetic VARIATION AND Stability Analysis of Rice Mutant Lines using AMMI Model Under NORMAL AND Drought Stress Conditions [Abstract] [Full text]

Dragana JUGOVIĆ, Predrag MILJKOVIĆ, Tatjana JEVTOVIĆ-STOIMENOV, Milena DESPOTOVIĆ, Višnja MADIĆ, Aleksandar MILIĆEVIĆ, Marija VUKELIĆ-NIKOLIĆ, Perica VASILJEVIĆ
ASSOCIATION OF TGF-β1 AND TNF-α GENES POLYMORPHISMS WITH THE KIDNEY SCARS FORMING RISK IN CHILDREN WITH VESICOURETERAL REFLUX [Abstract] [Full text]

Saeed MOHSENZADEH, Masoud SHEIDAI, Fahimeh KOOHDAR
POPULATION GENETIC STRUCTURE IN Plantago ovata var. decumbens WITH USING ISSR MARKERS [Abstract] [Full text]

Cengiz YÜCEDAĞ, Halil Barış ÖZEL, Sezgin AYAN ,Fulvio DUCCI, Vasilije V. ISAJEV, Muhidin ŠEHO
GROWTH CHARACTERISTICS OF Tilia tomentosa Moench. FROM DIFFERENT DISTRICTS IN THE REGIONS OF MARMARA AND WESTERN BLACK SEA IN TURKEY [Abstract] [Full text]

Velimir RADIĆ, Igor BALALIĆ, Goran JAĆIMOVIĆ,Aleksandra NASTASIĆ, Jasna SAVIĆ, Ana MARJANOVIĆ-JEROMELA
IMPACT OF DROUGHT AND SALT STRESS ON SEED GERMINATION AND SEEDLING GROWTH OF MAIZE HYBRIDS [Abstract] [Full text]

Ivana TOŠIĆ, Milan MIROSAVLJEVIĆ, Novo PRŽULJ, Miro STOŠIĆ
YIELD AND CONTENT OF NUTRIENT ELEMENTS IN VARIOUS CULTIVARS OF LETTUCE DEPENDING FROM PRODUCTION METHOD [Abstract] [Full text]

Corrigendum: Batos B., D. Miljković (2019): The phenotypic plasticity of of Picea omorika /panč./purkyne morphological pollen traits.- Genetika, Vol 51, No.1, 121-136. https://doi.org/10.2298/GENSR1901121B [Corrigendum] [Corrigendum Full text]

Jelena BOŠKOVIĆ
IN MEMORIAM
Prof dr Katarina Borojević [Text]

Published in „GENETIKA“ Vol. 51, No. 2 (2019), pp. 365-375

UDC 575.

https://doi.org/10.2298/GENSR1902365L

Orginal scientific paper

ESTIMATION OF GENETIC PARAMETERS OF AUTOSOMAL AND SEX-LINKED PRE-WEANING TRAITS IN MAKUIE SHEEP USING MULTIVARIATE ANALYSIS

Meysam LATIFI*¹ and Ali MOHAMMADI²

¹Genetics and Animal Breeding, University of Kurdistan, Kurdistan, Iran

²Genetics and Animal Breeding, University of Tabriz, Tabriz, Iran

Abstract

Latifi M. and A. Mohammadi (2019): Estimation of genetic parameters of autosomal and sex-linked pre-weaning traits in Makuie sheep using multivariate analysis.- Genetika, Vol 51, No.2, 365-375.

The objective of this study was to determine the genetic parameters of autosomal and sex-linked effects for birth weight (BW), weaning weight (WW), average daily gain from birth to weaning (ADG) and kleiber ratio (KR) of Makuie sheep. The data set used in this study was collected at Makuie sheep breeding station in West Azerbaijan province, Iran, between 1994 and 2011. The fixed effects included herd-year, sex (male or female), birth type (single or twin), and age of dam (five classes, 2-6 years old). The three multivariate linear animal models including direct (autosomal and sex-linked) and maternal effects were used to analyze data. The 2^nd model (including direct additive genetic effects of animal (autosomal and sex-linked) and permanent environmental effects), with the lowest AIC value was considered as the most appropriate model. Based on the most appropriate fitted model, direct autosomal and sex-linked heritabilities of BW, WW, ADG and KR were 0.12±0.04 and 0.03±0.03, 0.18±0.04 and 0.05±0.03, 0.37±0.04 and 0.04±0.03, and 0.52±0.05 and 0.07±0.04, respectively. The magnitude of sex-linked variances as a proportion of phenotypic variance obtained by the best model were 4%, 4.5%, 3% and 5.7% for BW, WW, ADG and KR, respectively. Also, the autosomal and sex-linked chromosomes, maternal permanent environmental and phenotypic correlations were ranged from -0.20 to 0.96, 0.11 to 0.84, -0.34 to 0.89 and -0.20 to 0.91, respectively. The results of this study indicated that the sex-linked chromosomes have an affect like maternal effects, and it could contribute to a more accurate estimation of the direct autosomal heritability.

Keywords: Sheep, Autosomal and sex-linked heritabilities, Pre-weaning traits, Multivariate analysis

Corresponding author: Meysam Latifi, Genetics and Animal Breeding, University of Kurdistan, Kurdistan, Iran, Tel. +98 918 406 9970; fax: +98 813 450 6128. E-mail: mlganjnameh@gmail.ocm

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Published in „GENETIKA“ Vol. 51, No. 2 (2019), pp.377-387

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1902377B

Original scientific paper

YIELD AND QUALITY PARAMETERS OF HOKKAIDO TYPE PUMPKINS GROWN IN SERBIA

Milka BRDAR-JOKANOVIĆ^1*, Anamarija KOREN¹, Branka LJEVNAIĆ-MAŠIĆ², Biljana KIPROVSKI¹, Vladimir SIKORA¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Abstract

Brdar-Jokanović M., A. Koren, B. Ljevnaić-Mašić, B. Kiprovski, V. Sikora (2019): Yield and quality parameters of hokkaido type pumpkins grown in Serbia.- Genetika, Vol 51, No.2, 377-387.

The aim of this study was to assess a set of 40 accessions of Hokkaido type pumpkins (Cucurbita maxima Duchesne) in terms of the traits of agronomic importance and the basic fruit flesh quality parameters, with the emphasis on Serbian environmental conditions. The pumpkins are a part of the larger Cucurbita collection belonging to the Institute of Field and Vegetable Crops from Novi Sad, where the two-year (2016, 2017) field trial was conducted. The accessions significantly differed in all analyzed parameters. The average values for the traits of agronomic importance were: yield per plant 3.56 kg, fruit weight 1.84 kg, number of fruits per plant 2.03, and anthesis date 180.29. Length of the main stem was in most cases short. Among fruit flesh quality parameters; the average dry weight content was 11.49%, sugars content 43.32 mg/g fresh weight, carotenoids content 50.12 mg/kg fresh weight, proteins content 4.04 mg/g fresh weight, total soluble solids content 8.63 °Brix, refractive index 1.347, and pH 6.98. The highest coefficients of variation for agronomic and quality traits were those calculated for yield per plant and carotenoids content. The pumpkins performed better in the first season of the experiment due to more favorable weather, firstly temperature, conditions. The most pronounced adverse effect of high temperatures occurring in 2017 was the one expressed on the content of carotenoids, which was on average 48.4% lower when compared to 2016. Principal component analysis was employed to clarify the relationships among the investigated parameters, and to distinguish those with the most important contribution to the pumpkin variability. The analysis revealed strong correlations among yield per plant, fruit weight and length of the main stem. Carotenoids content and pH also correlated to yield, while the other quality parameters correlated to each other. Defining both first and second principal components, yield per plant and fruit weight were the parameters with the highest contribution to the variability among the assessed pumpkins. Concerning quality parameters, fruit flesh dry weight contributed the most to the variability. Therefore, the strategy for breeding Hokkaido type pumpkins adapted to Serbian environmental conditions should be based on combining irrespectively selected high-yield and high-quality accessions.

Keywords: Cucurbita maxima, Hokkaido pumpkin, PCA, quality parameters, yield

Corresponding author: Milka Brdar-Jokanović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 780 365, Fax: 381 21 780 198, E-mail: milka.brdar@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 51, No. 2 (2019), pp.389-403

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR1902389G
Original scientific paper

THE molecular characterization of AN EXTENDED mulberry germplasm BY SSR markers

Beatriz GarcIa-GÓmez¹, Heidy GonzÁlez-ALVAREZ², Celia MartÍnez-Mora³, José Luis Cenis³, María del Carmen Pérez-Hernández², Yamila Martínez-Zubiaur², Pedro MartÍnez-GÓmez^1*

¹Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Espinardo, Murcia, Spain

²CENSA, La Habana, Cuba

³Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca (Murcia), Spain

Abstract

Garcia-Gómez B., H. Gonzalez-Alvarez, C. Martínez-Mora, J. Luis Cenis, M. del Carmen Pérez-Hernández, Y. Martínez-Zubiaur, P. Martínez-Gómez (2019): The molecular characterization of an extended mulberry germplasm by SSR markers. - Genetika, Vol 51, No.2, 389-403.

The objective of this work is the molecular characterization using nuclear SSR markers of different mulberry (Morus alba L.) cultivars from different countries including Cuba, Costa Rica, Brazil, South Korea, Ethiopia, China, Japan, Italy and Spain to deep in the dissemination through the world of this species. Results established the value of SSR markers for distinguishing different genetic lineages and characterize an extensive and largely gene pool available to mulberry cultivars. The results revealed the presence of 53 different alleles from the 12 SSR analysed in the 37 assayed genotypes. The Japanese, Italian and Spanish cultivars formed a separated group in which only 5 genotypes were present in the Cuban germplasm bank. On the other hand the Cuban cultivars ‘Cuba 2’ and ‘Cuba 3’ propagated from Chinese parents were grouped in the same branch, which suggests that these cultivars retain the genomic characteristics of their selection. It was also possible to relate the genotypes of cultivars ‘Tigriada’ and ‘Acorazonada’ present in Costa Rica as well as to evaluate other cultivars for their use in silkworm feeding. Molecular results also evidenced the dissemination of the cultivated mulberry specie from China around the world, the introduction in South Korea and Japan in the first dissemination step and the later wide dissemination of modern mulberry to the Mediterranean area and later to South America and the Caribe area.

Keywords: Dissemination, Genetic diversity, Microsatellites, Morus alba, Origin

Corresponding author: Pedro Martínez-Gómez, Departamento de Mejora Vegetal, CEBAS-CSIC, E-30100 Espinardo, Murcia, Spain, Phone: 34-968-39-6200; Fax: 34-968-39-6213; E-mail: pmartinez@cebas.csic.es

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Published in „GENETIKA“ Vol. 51, No2(2019), pp. 405-416

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902405P
Original scientific paper

GENETIC DIVERSITY OF CUCUMBER GENOTYPES REVEALED BY SSR MARKERS AND AGRONOMIC TRAITS

Kalidas PATI^1*, Anilabh Das MUNSHI², Manjusha VERMA³, Tusar Kanti BEHERA²,

Lalit ARYA³

¹ICAR-Central Tuber Crops Research Institute, Regional Centre, Bhubaneswar, Odisha 751019, India

²Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

³Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India

Abstract

Pati K., A.D. Munshi, M. Verma, T. K. Behera, L. Arya (2019): Genetic diversity of cucumber genotypes revealed by SSR markers and agronomic traits.- Genetika, Vol 51, No.2, 405-416.

Understanding the level of genetic diversity and structure of landraces is essential for economical use of genetic resources. In the present study, we investigated the genetic diversity of 36 representative sample of cucumber genotypes based on 10 quantitative traits and 17 polymorphic simple sequence repeat (SSR) markers. Our result revealed variability in flowering behaviour, growth habit and fruit characters. We found that the traits, days to opening of first female flower varied from 39.03 to 51.94, fruit length 6.09 to 30.01(cm), fruit weight 104.39 to 277.05(g) and yield per plant 699.38 to 1670.93(g). Genetic distance based on 17 SSR markers among 36 genotypes was quantified ranging from 0.03 to 0.70 indicated a wide diversity among the genotypes selected for evaluation in the present study. These primers produced 2-4 number of alleles with an average 2.76. Polymorphism information content varied from 0.005 to 0.550 with an average of 0.348. The observed heterozygosity mean was 0.098, while gene diversity or expected heterozygosity was 0.625 to 0.057. The distinct genotypes found in this study based on morpho-molecular characters will great interest to cucumber breeder for selection of diverse parent or production of mapping population.

Keywords: Cucumber, Genetic diversity, Simple sequence repeat (SSR), Agronomic traits

Corresponding author: Kalidas Pati, ICAR- Central Tuber Crops Research Institute, Regional Centre, Bhubaneswar, (Odisha) 751019, India. Phone: +917377914855,email:kalidas9555@gmail.com

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Published in „GENETIKA“ Vol. 51, No.2 (2019), pp. 417-428

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902417K

Original scientific paper

MORPHOLOGICAL, PHYSIOLOGICAL AND PRODUCTIVITY RESPONSE IN GARDEN PEA GENOTYPES DURING HIGH TEMPERATURE STRESS

Slavka KALAPCHIEVA*, Elena TOPALOVA, Valentina PETKOVA

Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria

Abstract

Kalapchieva S., E. Topalova, V. Petkova (2019): Morphological, physiological and productivity response in garden pea genotypes during high temperature stress.- Genetika, Vol 51, No.2, 417-428.

Changes in morphological characteristics, productivity and fluorescence emission parameters at normal and high temperature values under field conditions of 10 varieties and lines of the species Pisum sativum L. from the collection of MARITSA Vegetable Crops Research Institute were established. The influence of high temperature on the condition and functional activity of the photosynthetic apparatus (PSA) was studied by analyses of chlorophyll fluorescence characteristics of photosystem II (PSII) in the pea leaves. Plants were subjected to stress from high temperature during the reproductive period (bud formation - flowering phases), which is considered particularly critical for pea plants. The morphological performances and productivity have been influenced by climatic characteristics of the years. The screening for thermostability of the photosynthetic apparatus to the high temperature characterizes Skinado, Faboundo and line 95/4 as the most tolerant on the basis of analysis of fluorescence induction parameters. The most sensitive are lines 72/2 and 72/9. Varieties of Skinado and Fabundo respond with higher yields of green pods and grains in the conditions of the first year, categorized as hot compared to 2013. We consider that the indicated accessions are suitable for the pea-breeding programme to high temperature stress tolerance.

Keywords: pea, breeding, abiotic stress, reproductive period, yield

Corresponding author: Slavka Kalapchieva, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse Str. 32, 4003 Plovdiv, Bulgaria, Tel.:+35932952296, Fax:+35932960177, e-mail: s_kalapchieva@abv.bg

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Published in „GENETIKA“ Vol. 51, No. 2 (2019), pp. 429-445

Serbia

UDC 575.530

https://doi.org/10.2298/GENSR1902429G

Original scientific paper

PATH ANALYSIS FOR SEED YIELD IN SESAME (Sesamum indicum L.) INOCULATED/NON-INOCULATED WITH MYCORRHIZAL FUNGI UNDER DROUGHT STRESS

Esmaeil GHOLINEZHAD^1,*and Reza DARVISHZADEH²

¹Department of Agricultural Sciences, Payame Noor University. Tehran, Iran

²Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran

Abstract

Gholinezhad E.and R. Darvishzadeh (2019): Path analysis for seed yield in sesame (Sesamum indicum L.) inoculated/non-inoculated with mycorrhizal fungi under drought stress.- Genetika, Vol 51, No.2, 429-445.

In order to determine the effective traits to improve seed yield in sesame (Sesamum indicum L.), an experimental using factorial split plot design was conducted with three replications in the experimental field of Agricultural Research Center, West-Azerbaijan (Saatloo Station) in the 2015 and 2016 cropping seasons. The main plots (factor A and B) consisted of three different levels of irrigations (normal irrigation: irrigation after 70 mm evaporation of crop or ETc, moderate drought stress: irrigation after 90 mm ETc and severe drought stress: irrigation after 110 mm ETc) and factor B included three levels: two species of mycorrhizal fungi Glomus mosseae, Glomus intraradices and non-inoculated (control). Sub plots (factor C) consisted of eight commercial cultivars of sesame. The results showed high heritability most studied traits under different levels of drought stress, except for productivity effort. Estimation of variance components showed that under optimum conditions, all studied traits had high heritability and were less affected by environment. Path analysis showed positive significant correlations between seed yield and all studied traits. Progress in yield components can therefore increase seed yield in sesame. In general the research showed that the number of seed per capsule is one of the most important indicators of sesame seed yield under optimum irrigation. Also the traits such as 1000-seed weight and number of capsules per plant can be used as an option index in breeding programs for enhancing seed yield and selection of genotypes under optimum irrigation conditions, due to the high positive direct effect on seed yield. Under moderate and severe drought stress, the effective traits on seed yield were different in different species of mycorrizal.

Keywords: correlation, genetic variance, heritability, path analysis, sesame.

Corresponding author: Esmaeil Gholinezhad, Department of Agricultural Sciences, Payame Noor University. Tehran, Iran, gholinezhad1358@yahoo.com

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Published in „GENETIKA“ Vol. 51, No.2(2019), pp.447-461

Serbia

UDC 575.633.11

https://doi.org/10.2298/GENSR1902447K

Original scientific paper

LINE × TESTER ANALYSES FOR ANTHER CULTURE RESPONSE OF BREAD WHEAT (Triticum aestivum L)

Imren KUTLU^1*, Zeynep SIREL², Ozcan YORGANCILAR², Aysel YORGANCILAR²

¹Eskisehir Osmangazi University, Faculty of Agriculture, Department of Biosystem Engineering, 26160 Eskisehir/Turkey

²Transitional Zone Agricultural Research Institute, 26160 Eskisehir/Turkey

Abstract

Kutlu I., Z. Sirel, O. Yorgancilar, A. Yorgancilar (2019): Line × tester analyses for anther culture response of bread wheat (Triticum aestivum L.- Genetika, Vol 51, No.2, 447-461.

The aim of this study was to investigate the gene effects that play a role in the inheritance of the anther culture response of the F₁ and F₂ populations obtained from the hybridization between the seven doubled haploid (DH) wheat line and four bread wheat cultivar using line × tester method as well as, to determine suitable parents and promising hybrids. Eleven parents and their twenty-eight hybrid combinations were examined for androgenic properties related to anther culture response such as the number of callus, green, albino, haploid, spontaneous DH and fertile plants. The obtained data were evaluated by the combining ability analysis calculated using the line × tester method and by estimating the heritability degrees. According to the results, for all traits examined, the non-additive gene effects were found as significant and narrow sense heritability at a low level. Significant combining ability effects have been identified for some parents and hybrids regarding traits examined. DH19, DH22, and Harmankaya-99 have been identified as suitable parents to develop the androgenic traits, while crosses formed between them have been determined as promising. A total of 444 plants (142 F₁, 302 F₂) were transferred to the greenhouse from the plants obtained from the anther culture, and 205 (68 in F₁, 137 in F₂) were fertile. Ninety-three of the fertile plants were obtained from the parents. The seeds of 112 DH plants obtained from hybrids were replicated to be used in breeding programs.

Keywords: Androgenic Traits, Combining Ability, Doubled Haploid, Fertile Plants, Heritability.

Corresponding author: Imren Kutlu, Eskisehir Osmangazi University, Faculty of Agriculture, Department of Biosystem Engineering, 26160 Eskisehir/Turkey, Email: ikutlu@ogu.edu.tr, Phone: +90 222 3242990, Fax: +90 222 3242991

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Published in „GENETIKA“ Vol. 51, No.2 (2019), pp.463-472

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902463P

Original scientific paper

INHERITED THROMBOPHILIC RISK FACTORS IN SERBIAN BREAST CANCER PATIENTS

Iva PRUNER¹, Branko TOMIC^1*, Marija DRAGOJEVIC¹, Maja GVOZDENOV¹,

Mirjana KOVAC^2,3, Dragica RADOJKOVIC¹, Valentina DJORDJEVIC ¹

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia

²Faculty of Medicine, University of Belgrade, Serbia

³Blood Transfusion Institute of Serbia, Hemostasis Department, Belgrade, Serbia

Abstract

Pruner I., B. Tomic, M. Dragojevic, M. Gvozdenov, M. Kovac, D. Radojkovic, V. Djordjevic (2019): Inherited thrombophilic risk factors in Serbian breast cancer patients.- Genetika, Vol 51, No.2, 463-472.

Breast cancer is the leading cause of cancer-related death among women. An increased burden of thrombotic events among breast cancer patients, leading to higher mortality and morbidity rates, is well established. There are a number of genetic risk factors associated with thrombosis, but their contribution to thrombotic tendencies in patients with cancer is not completely elucidated. We aimed to investigate possible role of FV Leiden, FII G20210A, MTHFR C677T and PAI-1 4G/5G gene variants in etiopathology of breast cancer and accompanying thrombosis in cohort of Serbian patients. Our study included 316 subject divided in three groups: breast cancer patients with (97) or without (99) accompanying thrombosis and healthy control group (120). According to our results, the prevalence for all four prothrombotic gene variants were similar in cancer patients with and without thrombosis and no statistically significant difference was observed between these groups. We detected lower frequency of MTHFR 677TT genotype in breast cancer patients when compared to control group (P=0.014; OR=0.145 (95%CI 0.031-0.679)), indicated that MTHFR C677T homozygosity could play a protective role in breast cancer susceptibility. Our study noted the lack of association between common prothrombotic gene variants and increased prothrombotic risk in Serbian breast cancer patients. Also, our results point out possible role of MTHFR 677TT genotype in etiology of breast cancer, but further studies on larger cohort of patients are needed.

Keywords: breast cancer, venous thromboembolism, genetic risk factors, common prothrombotic mutations

Corresponding author: Branko Tomic, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11010 Belgrade, Serbia; Tel: +381113976658; Fax: +381113975808; e-mail: kobran@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 51, No.2 (2019), pp. 479-494

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902479G

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY OF Asparagus racemosus Willd. FROM DIFFERENT AGRO-ECOLOGICAL ZONES OF KERALA USING RAPD MARKERS

Chithra Madhanamohanan GEETHA¹ and Elenjikkal Avarachan SIRIL^2*

¹Department of Biotechnology, University of Kerala, Kariavattom, Thiruvananthapuram- 695581;

²Department of Botany, University of Kerala, Kariavattom, Thiruvananthapuram-695581

Abstract

Geetha C. M. and E. A. Siril (2019): Assessment of genetic diversity of Asparagus racemosus willd. from different agro-ecological zones of Kerala using RAPD markers- Genetika, Vol 51, No.2, 479-494.

Twenty accessions of Asparagus racemosus from ten agro-ecological zones of Kerala were evaluated for genetic diversity using Random Amplified Polymorphic DNA markers. Twenty random primers were selected based on reproducibility and clarity of bands. A total of 1209 bands were scored out of which, 1181 loci were found to be polymorphic (97.75%). Efficiency parameters of primers viz., Total Number of Loci, Total Number of Polymorphic Loci, Percentage of Polymorphism, Polymorphism Information Content (PIC), Resolving Power, Marker Index and Number of Unique Bands were estimated. Value of PIC varied from 0.059 to 0.209 and OPI19 is proved to be most polymorphic marker. Evaluation of relationship among seven efficiency parameters revealed positive and significant (P<0.01) correlation of Total Number of Loci with Total number of Polymorphic Loci, Resolving Power and Unique Bands. Jaccard’s similarity coefficient varied from 0.118 to 0.566. It indicates presence of large genetic variations within the accessions. The UPGMA dendrogram data revealed genetic relationships within accessions and there is no geographic isolation in the clustering. The present observations suggest that RAPD markers can be used as an effective tool for deriving intra-species genetic diversity among A. racemosus accessions.

Keywords: Efficiency parameters, Genetic diversity, Polymorphic Information Content (PIC), RAPD, UPGMA, Asparagus racemosus.

Corresponding author: Elenjikkal Avarachan Siril, Department of Botany, University of Kerala, Kariavattom, Thiruvananthapuram-695581, easiril@yahoo.com

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Published in „GENETIKA“ Vol. 51, No. 2 (2019), pp. 473-478

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902473B

Original scientific paper

ASSOCIATION OF SLC11A1 GENE AND PRODUCTION TRAITS USING LOGISTIC REGRESSION

Masoumeh BAGHERI* and Azadeh ZAHMATKESH

Department of Genomics and Genetic Engineering, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Araj, Alborz, Iran

Abstract

Bagheri M. and A. Zahmatkesh (2019): Association of SLC11A1 gene and production traits using logistic regression.- Genetika, Vol 51, No.2, 473-478.

SlC11A1 gene is a potential candidate gene related to susceptibility to a wide range of intracellular pathogens. In this research, we used logistic regression analysis in a case-control study to find the relationship between SLC11A1 gene and estimated breeding values of production traits (estimated breeding values of protein and fat yield, and protein and fat percentage) in Holstein dairy cattle in a selective genotyping method. Samples have been genotyped based on clinical mastitis data using PCR-SSCP method, previously. The contrast between heterozygous genotype and both homozygous genotypes showed that despite of significant relationship between clinical mastitis and SLC11A1, there was no negative effect of the gene of interest on production traits. Hence, considering the unfavorable relationship between clinical mastitis and production traits, selection of SLC11A1 gene will have no unfavorable effect on production traits, and it will not disorganize selection breeding programs based on health data.

Keywords: SLC11A1 gene, protein percentage, fat yield, logistic regression, mastitis.

Corresponding author: Masoumeh Bagheri, Department of Genomics and Genetic Engineering, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Hesarak, Karaj, Alborz, Iran.Email: m.bagheri@rvsri.ac.ir

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 495-510

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902495K

Original scientific paper

BLACK AND YELLOW SOYBEAN: CONTRIBUTION OF SEED QUALITY TO OXIDATIVE STRESS RESPONSE DURING PLANT DEVELOPMENT

Biljana KIPROVSKI¹*, Jegor MILADINOVIĆ¹, Anamarija KOREN¹, Đorđe MALENČIĆ²,Maja MIKULIČ-PETKOVŠEK³

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad,

Novi Sad, Serbia

³Department of Agronomy, Chair for Fruit, Wine and Vegetable Growing, Biotechnical faculty, University of Ljubljana, Ljubljana, Slovenia

Abstract

Kiprovski B., J. Miladinović, A. Koren, Đ. Malenčić,M. Mikulič-Petkovšek (2019): Black and yellow soybean: contribution of seed quality to oxidative stress response during plant development. - Genetika, Vol 51, No.2, 495-510.

The purpose of this paper was to compare quality of seeds of two black and yellow soybean and the response of plants developed from these seeds to oxidative stress during vegetation and reproduction period. Content of carbohydrates: cellulose, starch, total and reduced sugars, as well as oil and protein content varied among all varieties, irrespective the colour. Bearing in mind all differences in seed quality, as well as response to oxidative stresses during development, black cultivars could be proposed as an excellent source of phenolic compounds (flavan-3-ols, antocyanins, genistein, glycitein, quercetin, laricitrin and isorhamnetin derivatives). Due to extreme fluctuation in precipitation amounts in the last years, information on the better performance of soybean varieties in oxidative stress conditions is of great importance to organic and conventional production of this cultivar.

Keywords: antioxidants, black and yellow soybean; HPLC-MS; lipid peroxidation; polyphenols; seed quality

Corresponding author: Biljana Kiprovski, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia biljana.kiprovski@ifvcns.ns.ac.rs, tel.+38121780365, fax.+38121780198

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Published in „GENETIKA“ Vol. 51, No.2(2019), pp. 511-524

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902511G

Original scientific paper

GENETIC ANALYSIS OF HEMAGGLUTININ PROTEINS OF H3 AND H1 SUBTYPES IN KAZAKHSTAN

Dilyara GRITSENKO ^1,2,3*, Aleksandr POZHARSKY⁴, Nina DERYABINA¹,

Aliya KASSENOVA⁵, Nurbol GALIAKPAROV¹

¹Laboratory of molecular biology, Institute of plant biology and biotechnology, Almaty, Kazakhstan

²Laboratory of molecular genetics and biochemistry, Institute of plant protection and quarantine, Almaty, Kazakhstan

³Department of molecular biology and genetics, Al-Farabi Kazakh National University, Almaty, Kazakhstan

⁴Asfendiyarov Kazakh National Medical University, Kazakhstan

⁵Nazarbayev University, Astana, Kazakhstan

Abstract

Gritsenko D., A. Pozharsky, N.Deryabina, A. Kassenova, N. Galiakparov (2019): Genetic analysis of hemagglutinin proteins of H3 and H1 subtypes in Kazakhstan.- Genetika, Vol 51, No.2, 511-524.

The influenza is one of the most dangerous and widespread infectious diseases on the planet. A natural reservoir of the influenza A virus is wild waterfowl, which subsequently contribute to the spread of viral infection among domestic animals. Currently, different influenza A subtypes were isolated from various animal species and their genetic diversity were investigated with the subsequent possibility to predict the places of outbreaks and the transfer between species. An investigation of genetic diversity of influenza A virus is also important for a timely response by developing suitable vaccines to the emergence of new strains. In the work, we investigated two subtypes of hemagglutinin (H3, H1) from wild waterfowl in the Republic of Kazakhstan (RK). This work was aimed to determine the homology between these subtypes and currently known isolates from the NCBI database. H3N8 isolates from Kazakhstan were located in a monophyletic group together with isolates from Mongolia and Altai according to phylogenetic analysis of the hemagglutinin sequences . The hemagglutinin homology of H3N8 isolates from Kazakhstan and A/eq/Richmond/1/2007 vaccine strain was 86.07% and with A/eq/Ohio/2003 vaccine strain was 86, 24%. In the case of H1N1 isolates from Kazakhstan, the highest hemagglutinin homology was with isolates from Europe. The homology with the A/California/07/2009 (H1N1) vaccine strain was 81.27%. Important amino acids of cleavage and receptor binding sites were not variable in both H3 and H1 subtypes. The investigation of antigenic sites showed presence of variations in all five sites for H3 subtype and in 4 sites for H1.

Keywords: Influenza virus, subtype, genetic diversity, hemagglutinin, vaccine.

Corresponding author: Gritsenko Dilyara:phone +77073865386, e.mail:d.kopytina@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 525-537

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902525K

Original scientific paper

RELATIONSHIPS BETWEEN SURVIVAL IN WINTER COLDS AND SOME MORPHOLOGICAL AND TECHNOLOGICAL CHARACTERISTICS IN SAFFLOWER GENOTYPES

Hasan KOÇ

Bahri Dagdas International Agricultural Research Institute, Konya, Turkey

Abstract

Koç H. (2019): Relationships between survival in winter colds and some morphological and technological characteristics in safflower genotypes.- Genetika, Vol 51, No.2, 525-537.

This study was conducted in the production seasons of 2013-2014 and 2015-2016 on test fields at Bahri Dagdaş International Agricultural Research Institute in the Province of Konya, Turkey, where winters are quite harsh, to determine the relationships between tolerance to winter colds in safflower and some morphological and technological characteristics. Of the 112 genotypes, 11 genotypes which had been selected in previous years on the basis of their tolerance to winter conditions and 4 varieties (Remzibey, Dinçer, Linas, Balcı), which were registered as spring plants in Turkey, were used in the study as materials. Trial sowing was performed in the first week of October in autumn. The survival rates of the genotypes in winter colds were determined at -14.2°C in the 2013-2014 production season and at-17.3°C in the 2015-2016 production season. Root lengths and plant habitus were measured in autumn in the rosette stage (6-8 weeks after emergence). Spring sowing was conducted in the first week of April to determine the stem elongation time of the genotypes and thus their stem elongation times were specified. Oil and seed coat ratios, on the other hand, were determined before sowing for fear that they might be completely destroyed by cold. In standard varieties, survival rates varied between 1.25 % and 40.7 % at -14.2°C without a snow cover in the 2013-2014 production season, whereas in tolerant genotypes this rate was between 94 % and 99.5 %. In the 2015-2016 production season, the survival rate in standard varieties that were sensitive to winter colds varied between 0 % and 2 % at-17.3°C without a snow cover, whereas the survival rates in the most tolerant genotypes varied between 85.9 %, 77.6 % and 54.4 %, respectively. A significant correlation was determined between the survival rate and plant habitus and oil content. It was found that the winter-tolerant lines had a more prostrate plant habitus and lower oil contents in the rosette stage. A positive and significant relationship was found between winter survival and root length, seed coat thickness and the length of stem elongation time. Keywords: safflower, winter survival, cold tolerance, plant habit

Corresponding author: Hasan KOÇ, Bahri Dagdas International Agricultural Research Institute, Konya, Turkey, POBox: 125, Karatay/Konya, Turkey, Office Phone: +90 332 355 2 90,: e-mail: koc175@hotmail.com

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Published in „GENETIKA“ Vol. 51, No.2(2019), pp. 539-549

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902539R

Original scientific paper

MORPHO-BIOCHEMICAL CHRACTERIZATION OF AMLA (Phyllanthus emblica L.) AND TAMARIND (Tamarindus indica L.) GERMPLASM FROM PAKISTAN

Malik ASHIQ RABBANI¹, Sohail AHMAD JAN*², Sadar UDDIN SIDDIQUI¹, Abdul GHAFOOR¹ and Zahoor AHMAD

¹Plant Genetic Resources Program, Bio-Resources Conservation Institute, National Agricultural Research Centre, Islamabad, Pakistan

²Department of Biotechnology, Hazara University Mansehra, Khyber Pakhtunkhwa, Pakistan

Abstract

Rabbani Ashiq M., S. Ahmad Jan, S. Uddin Siddiqui, A. Ghafoor and Z. Ahmad (2019): Morpho-biochemical chracterization of amla (Phyllanthus emblica L.) and tamarind (Tamarindus indica L.) germplasm from Pakistan.- Genetika, Vol 51, No.2, 539-549.

Amla (Phyllanthus emblica L.) and Tamarind (Tamarindus indica L.) are the least researched food plants in Pakistan although they have ample uses in food and herbal industry. Amla is being cultivated and observed as wild in most parts of the country including Sindh, Punjab and Khyber Pakhtunkhwa, whereas Tamarind is mostly confined to tropical areas mainly at Karachi. This study was focused to identify and collect the germplasm of both the species. Among all the collecting sites two types (Banarasi and Sheesha) of Amla were observed at farmers’ fields, whereas wild or Desi types were under natural cultivation. Various important qualitative and yield related important quantitative traits were recorded. For biochemical characterization standard Sodium Dodecyl Sulphate- Polacrylamide Gel Electrophoresis (SDS-PAGE) protocol was used. The seed proteins were separated by using 11.25% polyacrylamide gel. The plants at farmers’ fields were observed with a considerable level of variation. Low variation within Banarasi and Sheesha types might be due to limited number of mother plants by few commercial growers that needed to broaden through more germplasm collection/acquisition. High level of morphological diversity was observed among Amla and Tamarind genotypes. The protocol for total proteins in Amla did not exhibit good quality electrophoregram that is needed to refine for its practical utilization. The SDS-PAGE conducted for tamarind gave 11 protein bands and out of these six were polymorphic. The phylogenetic analysis classified all twenty Tamarind genotypes into seven diverged groups. Although most of the plants were similar on their phenotypic basis but the total proteins gave significant differences for most of the plants that indicated the diversity for genetic material and acclimatization to local environment.

Keywords:Collection, Local germplasm, Morpho-biochemical evaluation, Polymorphism, Propagation, SDS-PAGE

Corresponding author: Sohail Ahmad Jan, Department of Biotechnology, Hazara University, Mansehra, KP, Pakistan, E-mail: sjan.parc@gmail.com;sohailahmadjan3@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 551-570

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902551S

Original scientific paper

MULTI-ENVIRONMENTAL EVALUATION OF CHILLI GENOTYPES FOR YIELD AND YIELD COMPONENTS

Tejpal Singh SRAN and S. K. JINDAL*

Department of Vegetable Science, Punjab Agricultural University, Ludhiana (Punjab)-141004, India

Abstract

Sran Singh T. and S. K. Jindal (2019): Multi-environmental evaluation of chilli genotypes for yield and yield components.- Genetika, Vol 51, No.2, 551-570.

Forty three chilli genotypes including one check Punjab Sindhuri were evaluated for yield and its related attributes to determine stability and adaptability under three different environments (November transplanted 2016, February transplanted 2017 and April transplanted 2017) at Punjab Agricultural University, Ludhiana. The mean square (MS) due to genotypes was significant for all the traits studied except primary number of branches. The MS due to genotype × environment interaction was also found significant for all the traits studied. The genotype S 343 was identified as promising for fruit yield plant^-1and plant height in all the three environments followed by PG 417 and PL 412 while on the basis of regression, the genotype PAU 114 was found adaptable across the environments for fruit yield and fruit weight. It was observed that the genotype FL 201 had the longest fruit, more fruit width and thicker pericarp over all the environments. The promising genotypes found in the study could have the potential of being commercially exploited at farmer’s field especially for early and late season.

Keywords: Chilli, genotypes × environment, mean square, significant, fruit yield

Corresponding author: S. K. Jindal, Department of Vegetable Science, Punjab Agricultural University, Ludhiana (Punjab)-141004, India, E-mail: saleshjindal@pau.edu , +91-8360121290

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 571-584

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902571K

Original scientific paper

Genetic analysis of quantitative TRAITS of grass pea

(Lathyrus sativus L.) genotypes

Valentin KOSEV and Viliana VASILEVA*

Institute of Forage Crops, Pleven, Bulgaria

Abstract

Kosev V. and V. Vasileva (2019): Genetic analysis of quantitative traits of grass pea (Lathyrus sativus L.) genotypes.- Genetika, Vol 51, No.2, 571-584.

A comprehensive assessment of the quantitative traits of grass pea varieties and hybrids was done in order to inclusion in the selection process. A negative heterosis effect was found in almost all hybrids at two environment limits (seed density). The exception was LA5108 x BGE027129, which is characterized by a high heterosis effect on the number of nodules per plant. Dominant to over-dominant negative inheritance was found in F1 at BGE027129 x LA5108 for fresh root mass weight, aboveground mass weight and nodule weight per plant, and in LA5108 x BGE027129 for fresh leaves weight and fresh aboveground mass weight. LA5108 x BGE027129 shows a positive dominance and over-dominance in both, number and weight of nodules per plant. Non-allelic interactions occur in inheriting the number and weight of nodules in the hybrid combination BGE025277 x LA5108. The hybrids tested had the most pronounced positive transgressions on the fresh aboveground mass weight, number and weight of nodules. The common phenotypic manifestation of fresh root mass weight, fresh aboveground mass weight and nodule weight is highly genetically determined, and a larger effect can be expected from conducting mass selection on these signs in earlier hybrid offspring (F2 - F3). The assessment of the initial material makes it very likely to determine the appropriate productivity genotype and to speed up the process of creating new varieties of grass pea.

Keywords: grass pea, quantitative traits, dominant gene effect, heterosis, transgression, inheritance

Corresponding author: Viliana Vasileva, Institute of Forage Crops, 89 “General Vladimir Vazov” Str., 5800 Pleven, Bulgaria. E-mail: viliana.vasileva@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp.585-593

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902585K

Original scientific paper

FATTY ACID AND TRACE ELEMENT COMPOSITIONS OF THE SEEDS OF DIFFERENT Onobrychis viciifolia GENOTYPES

Mahmut Kaplan¹, Veysel Turan², Yusuf Murat KARDES¹, Aydin DAS³, Kagan KOKTEN⁴

¹Department of Field Crops, Faculty of Agriculture, Erciyes University, Kayseri, Turkey

²Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Bingol University, Bingol, Turkey

³Department of Animal Science, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey

⁴Department of Field Crops, Faculty of Agriculture, Bingol University, Bingol, Turkey

Abstract

Kaplan M., V. Turan, Y. M. Kardes, A. Das, K. Kokten (2019): Fatty acid and trace element compositions of the seeds of different Onobrychis viciifolia genotypes. - Genetika, Vol 51, No.2, 585-593.

Seed oil contents, fatty acid compositions and mineral contents of twenty Onobrychis viciifolia genotypes (sainfoin) were investigated in this study. The fatty acid composition of O. viciifolia genotypes had different saturated and unsaturated fatty acids. For saturated fatty acids, O. viciifolia genotypes contained palmitic and stearic acids as the major component and contained small amount of myristic and arachidic acids. The major unsaturated fatty acids were identified as oleic, linoleic and linolenic acids. Total saturated fatty acids contents varied between 10.50 and 14.28% and total unsaturated fatty acid contents varied between 85.72 and 89.50%. Co, Mn, Fe, Cu, Ni and Zn were detected in crop seeds in different amounts. The proximate analysis indicated that seed Co contents varied between 13.53 and 114.83 ppm, Mn contents between 28.14 and 97.20 ppm, Fe contents between 113.7 and 277.7 ppm, Cu contents between 7.19 and 12.24 ppm, Ni contents between 1.14 and 19.73 ppm and Zn contents between 26.03 and 52.39 ppm, respectively. The result of this study suggest that further studies are obligatory to invigorate in terms of animal nutrition and phytochemicals of O. viciifolia.

Keywords: Legume seeds, O. viciifolia, genotypes, fatty acid, trace elements

Corresponding author: Mahmut Kaplan,Department of Field Crops, Faculty of Agriculture, Erciyes University, Kayseri, Turkey, e-mail: mahmutkaplan5@hotmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 595-606

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902595A

Original scientific paper

YIELD COMPONENTS ANALYSES IN COTTON: G. hirsutum CULTIVARS WITH MULTIVARIATE STATISTICAL METHODS

Mahdi BADRI ANARJAN¹, Farshid TALAT^2*, and Amir FAYYAZ MOGHADDAM³

¹Plant Genomics Laboratory, Department of Plant Biotechnology, College of Life Sciences, Sejong University, Republic of Korea.

² Seed and Plant Improvement Research Department, West Azerbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia, Iran

³ Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Anarjan Badri M., F. Talat, and A. Fayyaz Moghaddam (2019): Yield components analyses in cotton: G. hirsutum cultivars with multivariate statistical methods.- Genetika, Vol 51, No.2, 595-606.

Cotton is the first and the main fibrous plant that it plays an important role in the creation of careers for individuals and development of textile industries in all over the world. Selection of a suitable cultivar for the West Azerbaijan region is important, since West Azerbaijan is the origin of cotton in Iran. This research conducted in order to evaluate of quantitative and qualitative traits of hopeful cotton cultivars under cold conditions in Urmia region. In this study eight cultivars evaluated with Varamin and Sahel as check cultivars in form of randomized complete block design with four replications in 2014 and 2015 (two cropping seasons) at Saatlou station of west Azerbaijan agricultural research, education, and extension organization. Results showed that K8802 cultivar is earliness cultivar and also is one of the best cultivars according to the traits such as the number of bolls per plant, sympodia per plant, final yield, seed cotton yield (yield of per plant), and qualitative traits. According to the combination analysis results, the interaction effect between treatment and year (treatment*year) for seed cotton yield, yield, and the number of bolls per plant at probability level (α=0.01) were significant. Stepwise multiple liner regression analysis revealed entrance of three variables, the number of bolls per plant, boll weight, and plant height entered in the model. Path analysis showed that direct effect of the number of bolls per plant with yield, indirect effect of the number of bolls per plant and boll weight with yield are the most in among all effects. Principal components analysis for two main components with the high value for variables showed that there is the number of bolls per plant in both components and this trait is one of the main traits in cotton studies. Therefore, the cotton breeders must work on the number of bolls per plant trait. Due to the results of this study in two years, K8802 cultivar suggests for cultivation in Urmia weather conditions.

Keywords: Cultivar suggestion, Fiber quality, Quantitative Properties, Yield

Corresponding author: Farshid Talat,Seed and Plant Improvement Research Department, West Azerbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Urmia, Iran, E-mail: f.talat@areeo.ac.ir

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 607-617

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902607A

Original scientific paper

HUMAN LEUKOCYTE ANTIGEN POLYMORPHISMS AS SUSCEPTIBILITY RISK FACTORS FOR END STAGE RENAL DISEASE

Dušica ADEMOVIĆ-SAZDANIĆ^1* andSvetlana VOJVODIĆ^1,2

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, Novi Sad, Serbia;

²University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia

Abstract

Ademović-Sazdanić D. and S. Vojvodić (2019): Human leukocyte antigen polymorphisms as susceptibility risk factors for end stage renal disease.- Genetika, Vol 51, No.2, 607-617.

Majority of the patients with chronic renal failure develop end-stage renal disease (ESRD). ESRD is a worldwide public health problem. That requires renal transplantation. Currently, many genome-wide association studies have suggested a potential association between human leukocyte antigen (HLA) and ESRD as uncovered relationship. This study is the first report from Serbia to find out the distribution of HLA -A, -B, -DRB1 specificity, two loci disequilibrium linkage between these HLA loci and possible association in renal transplant recipients from the region of Vojvodina, Serbia. From the same region, 230 ESRD patients who were waiting for kidney transplantation and 290 healthy controls were included in this study. Polymerase chain reaction-sequence specific primer (PCR-SSP) method were used to analyze the HLA polymorphisms (including HLA-A, HLA-B and HLA-DRB1 loci) in both ESRD patients and controls. The frequencies of alleles at these loci and two loci disequilibrium linkage coefficients were compared between ESRD patients and controls.The current work suggests that HLA DRB1*04 allele (odds ratio = 1.6484, 95 % CI = 1.0395–2.6138, P = 0.0325) may represent susceptibility risk factor for the development of ESRD in Serbian individuals. The highest two loci disequilibrium linkage coefficients in ESRD patients were found for B*18~DRB1*11 (D= 0.01583) and A*02 ~B*51 (D= 0.0145) and in controls for B*08~DRB1*03 (D=0.370) and A*01~DRB1*03 (D=0.02446), respectively, but without reaching significant levels. The results of our study suggests HLA DRB1*04 as a risk marker that might be involved with ESRD development. Further studies should be undertaken to analyze long-term results from kidney transplantation and clarify types and subtypes of alleles involved with ESRD progression.

Keywords: HLA, end stage kidney disease

Corresponding author: Svetlana Vojvodić, University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia, E-mail: svetlana.vojvodic021@gmail.com, svetlana.vojvodic@mf.uns.ac.rs

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 619-627

Serbia

UDC 575.

https://doi.org/10.2298/GENSR190261R

Original scientific paper

USEFULNESS OF THE 17-PLEX STR KIT FOR BOSNIAN MOUNTAIN HORSE GENOTYPING

Dunja Rukavina^1*, Amir Zahirović², Ćazim CRNKIĆ³, Mirela Mačkić-Đurović⁴, Adaleta Durmić-Pašić⁵, Belma Kalamujić Stroil⁵, Naris Pojskić⁵

^1*University of Sarajevo-Veterinary Faculty, Department of Biology, Sarajevo, B&H

²University of Sarajevo-Veterinary Faculty, Department of Internal Diseases, Sarajevo, B&H

³University of Sarajevo-Veterinary Faculty, Department of Animal Nutrition, Sarajevo, B&H

⁴University of Sarajevo-Faculty of Medicine, Center for Genetic, Sarajevo, B&H

⁵University of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, B&H

Abstract

Rukavina D., A. Zahirović, Ć. Crnkić, M. Mačkić-Đurović, A. Durmić-Pašić, B. Kalamujić Stroil, N. Pojskić (2019): Usefulness of the 17-plex STR kit for Bosnian mountain horse genotyping.- Genetika, Vol 51, No.2, 619-627.

In the present study modern technology of DNA extraction and automatic genotyping was applied in Bosnian and Herzegovinian autochthonous horse breed by using 17-Plex horse genotyping kit. The study was aimed at investigating usefulness of the 17-plex STR Kit for Bosnian mountain horse genotyping and establishing highly useful microsatellite markers system for genetic diversity studies in Bosnian mountain horse breed. Genomic DNA was extracted from whole blood collected from 22 unrelated Bosnian mountain horse specimens. A total of 95 alleles were detected. Average number of detected alleles per locus was 5.588, varying from 3 (HTG7) to 10 (ASB17). Average effective number of alleles was 3.603, fluctuating from 1.789 (HMS7) to 5.728 (HMS2). The observed heterozygosity ranged from 0.136 (HMS3) to 0.909 (ASB2) with a mean of 0.631. The results indicate that the studied population originates from the appropriate number of parent generations. The mean expected heterozygosity was 0.690, varying from 0.441 (HMS7) to 0.853 (ASB17) indicating high genetic variability within Bosnian mountain horse population. The PIC values ranged from 0.409 (HMS7) to 0.837 (ASB17) with a mean of 0.643, suggesting that 94.12% markers were quite informative in terms of their suitability for genetic diversity studies .The most polymorphic locus was HMS2 and the least polymorphic locus was HMS7. The inbreeding coefficient ranged from -0.030 (HMS7) to 0.807 (HMS3) with a mean of 0.077. Inbreeding coefficient values indicated no shortage of heterozygotes in Bosnian mountain horses. Deviation from Hardy-Weinberg equilibrium (p<0,05) was found in three loci (HTG10, HMS3 and ASB17). The applied set of 17 microsatellite markers proved to be sufficiently specific for use in genotyping of Bosnian mountain horse. Considering the values of H_O, H_E and PIC over 0.6, five microsatellite markers system (HTG4, AHT4, AHT5, ASB2, HMS2) is considered to be highly useful for genetic diversity studies in Bosnian mountain horse breed.

Keywords: Bosnian mountain horse, genetic diversity, microsatellite markers, polymorphism, 17-Plex horse genotyping kit

Corresponding author: Dunja Rukavina, Biologist, Ph.D., Department of Biology, University of Sarajevo-Veterinary Faculty, Zmaja od Bosne 90, 71 000 Sarajevo, B&H, phone: + 38733 729-100, fax: + 38733 617-850, e-mail: dunja.rukavina@vfs.unsa.ba

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 629-639

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902629A

Original scientific paper

DETERMINATION OF OPTIMAL MUTAGEN DOSAGE AND ITS EFFECTS ON MORPHO-AGRONOMIC TRAITS IN PUTATIVE MUTANTS OF ‘AMASYA’ APPLE

A.Nilgun ATAY^1,*, Ersin ATAY², Burak KUNTER³, K. Yaprak KANTOGLU³,

Nejdet KAPLAN⁴

¹TAGEM Fruit Research Institute, 32500, Egirdir, Isparta, Turkey

²Burdur Mehmet Akif Ersoy University, Food Agriculture and Livestock School, Department of Crop and Livestock Production, Horticulture Programme, Burdur, Turkey

³Turkish Atomic Energy Authority, Saraykoy Nuclear Research and Training Center (TAEA-SNTRC), Ankara, Turkey

⁴General Directorate of Plant Production, Ankara, Turkey

Abstract

Atay A. N., E. Atay, B. Kunter, K. Y. Kantoglu, N. Kaplan (2019): Determination of optimal mutagen dosage and its effects on morpho-agronomic traits in putative mutants of ‘Amasya’ apple.- Genetika, Vol 51, No.2, 629-639.

Mutation breeding is one of the efficient ways to create new genotypes. The starting material of this study was the ‘Amasya’, a Turkish apple cultivar. This study aimed to identify optimal mutagen dosage that is the most important prerequisite to create a proper mutant population, and evaluate the genetic variability for morpho-agronomic traits (e.g. tree height and fruit number) in the putative apple mutants of ‘Amasya’ irradiated at the optimal mutagen dosage. In the first year of the study, to determine the optimal mutagen dosage, dormant ‘Amasya’ scion woods were irradiated at 0, 10, 20, 30, 40, 50 and 60 gray dosages using ⁶⁰Co source. The optimal mutagen dosage was determined as 29.01 gray for ‘Amasya’ concerning the 50% shoot length reduction in comparison to the non-irradiated control plants. In the following year, more or less 2000 dormant ‘Amasya’ scion woods were irradiated at the optimal mutagen dosage to generate a mutant population of ‘Amasya’ apple cultivar. Mutation frequency based on the visual observations of plant abnormalities was observed. Then three years, genetic uniformity was analyzed through the growth of the mutant population, and mutation frequency (%) determined. These results indicated that there was considerable genetic variability in response to irradiation for evaluated traits. Most of the plants in the putative mutant population, nearly 80%, had a significantly shorter tree height and trunk cross-sectional area than standard ‘Amasya’. The mutants/genotypes identified in this study could be used improvement of new promising apple cultivars with good agronomical traits.

Keywords: fruit breeding, gamma irradiation, Malus domestica, Malus slyvestris, mutation breeding

Corresponding author: A. Nilgun Atay, TAGEM Fruit Research Institute, 32500, Egirdir, Isparta, Turkey, Tel.: +90 246 313 2420, fax: +90 246 313 2425, e-mail: nilguntuncer@hotmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp.641-660

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902641S

Original scientific paper

GENETIC ANALYSIS OF NUTRITIONAL TRAITS IN TROPICAL CARROT

(Daucus carota L.)

selvakumar, R.*¹, Pritam kalia², R.S., raje²

¹ICAR-Central Institute of Temperate Horticulture, KD Farm, Old Air Field, Rangreth-190 007, Jammu and Kashmir, India

²ICAR-Indian Agricultural Research Institute, New Delhi, India-110012

Abstract

selvakumar, R., P. kalia, R.S., raje (2019): Genetic analysis of nutritional traits in tropical carrot (Daucus carota L.).- Genetika, Vol 51, No.2, 641-660.

Carrot has wide color range particularly purple, orange, red and yellow dependent upon anthocyanins, β-carotene, lycopene and lutein content. Due to recent awareness of nutritional health security, there is increasing consumption of carrot by consumers as well as nutraceutical based industry owing to huge bioavailability of dietary vitamins, phytonutrients and bioactive compounds of carrots. Though, breeding for multinutritional rich carrot varieties, there is need to study and understand the genetic mechanism of nutritional traits viz., moisture, total solids, total soluble solids, total carotenoids, β-carotene, lycopene and total anthocyanin content. Keeping these in view, the present investigation was designed to study the six genetic parameter in six generation of twelve cross combinations. The six digenic parameter model results clearly revealed that additive [d], dominance [h], additive × additive [i], additive × dominance [j] and dominance × dominance [l] interactions governed nutritional traits in the crosses studied. The positive sign of dominance [h] and dominance × dominance [l] resulted into complimentary epistatic interactions controlling moisture content and total solids, total soluble solids, total carotenoids and β-carotene, lycopene (in all crosses except PM × IPC-122) and total anthocyanin content. From the higher frequency of allele dispersal [a+i] between the parents, it is evident that the selection process should be delayed until homozygosity is attained in advance generation which can be obtained through inter-mating of parents followed by cyclic recurrent selection and mass selection for nutritional traits with consecutive cross combinations. This epistatic parameter of genetic analysis would be effectively utilized for breeding strategy for improvement of nutritional traits in carrot.

Keywords: tropical carrot, allele, recurrent selection, mass selection, nutritional traits enrichment

Corresponding author: Selvakumar, R., ICAR-Central Institute of Temperate Horticulture, KD Farm, Old Air Field, Rangreth-190 007, Jammu and Kashmir, India, e-mail: selvakumarsingai@gmail.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 661-674

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902661F

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY AMONG TWO in vitro Aole barbadensis

AND Aloe littoralis REGENERATED PLANTS USING C-VALUE DNA OF FLOW CYTOMETRY

Farah FARAHANI¹*, Atena BAYANI², Zahra NOORMOHAMMADI²

¹Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

² Biology Department, School of Basic sciences, Science and Research Branch, Islamic Azad University (SRBIAU), Tehran, Iran

Abstract

Farahani F., A. Bayani, Z. Noormohammadi (2019): Assessment of genetic diversity among two in vitro Aole barbadensis and Aloe littoralis regenerated plants using C-value DNA of flow cytometry.- Genetika, Vol 51, No.2, 661-674.

Aloe belonging to the family Asphodelaceae family, formerly Liliaceae, is a genus of herbaceous and succulents. Shoot tip of 2-3 cm were collected from offshoot of Aloe barbadensis and Aloe littoralis, explants were surface sterilized, after were inoculated with MS medium containing various concentrations of BA (0.5, 1, 2 mg/L), IAA (0.5, 1 mg/L). The cultures were incubated at 25 ± 2°C under a 16 h photoperiod. In A. barbadensis and A. littoralis, the best treatment for highest shoot number and bud proliferation was MS medium containing 2 mg/L BA and 0.5 mg/L IAA. Maximum percentage of proliferated shoot buds (90% and 95%) from a single explant were obtained in MS medium after 4-5 weeks of the second and the first subcultures, respectively. Both of the species were adventitious root induced in MS medium containing 0.5 mg/L BAP and 0.5 mg/L IAA after the second subculture. Genome size of the regenerated plants in A. barbadensis and A. littoralis species were differed in different treatments (from 30.66 pg to 39.62 pg, 2C value). One sample T-test showed significant difference (P=0.001) between genome sizes of two species studied when A. littoralis showed higher C-value in comparison to A. barbadensis. Different genome sizes were also indicated among treatments and subcultures. The mixoploids identified in flow cytometry histograms by the presence of two or three peaks of in A. barbadensis and A. littoralis histograms in apple regenerated plants in different treatments and subcultures in treatment 2 (BA 1 mg/L, IAA 0.5 mg/L).

Keywords: Aole barbadensis, Aloe littoralis, C-value, Regeneration, Flow cytometry

Corresponding author: Farah Farahani, Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran, Tel : +989122778171, farahfarahani2000@yahoo.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp 675-686

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902675G

Original scientific paper

MITOCHONDRIAL 16S rDNA PROFILING AND PHYLOGENETIC ANALYSIS SUGGEST GENETIC DIVERSITY OF ASH WEEVIL (Stereonichus fraxini De Geer)

IN SERBIA

Vladislava GALOVIC*, Milan DREKIĆ, Sreten VASIĆ, Siniša ANDRAŠEV, Saša PEKEČ,

Dejan V. STOJANOVIĆ, Verica VASIĆ

University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia

Abstract

Galovic V., M. Drekić, S. Vasić, S. Andrašev, S. Pekeč, D. Stojanović, V.Vasić (2019): Mitochondrial 16s rDNA profiling and phylogenetic analysis suggest genetic diversity of ash weevil (Stereonichus fraxini de Geer) in Serbia.- Genetika, Vol 51, No.2, 675-686.

This study contributes to knowledge of ash weevil (Stereonychus fraxini De Geer) molecular taxonomy, phylogeny and genetic diversity. Adult and larvae stages of insect were collected from several locations covering northern and central part of Serbia cojoined with homologous sequences with respect to their different geographic origin and hypothesis of their evolutionary relationships. Due to its slow rates of evolution the gene region that covers mitochondrial 16S rDNA, was choice for sequence profiling and phylogenetic reconstruction of ash weevil in correspondence with sequences of related tribes Cionini. Phylogenetic analyses demonstrating clear separation of the native weevil populations and the Cionini tribes. Even though bioinformatic tools confirm that all native specimens belong to species Stereonychus fraxini, different profile of the mitochondrial 16S rDNA in the clade of Serbian specimens indicate intraspecific genomic rearrangement in one specimen detached it to northern geographic position. Those particular specimens invade also different Fraxinus species. Genetic distinctness of other imagos from this particular individual proved by indels and point mutations found in their sequences. By screening the mitochondrial 16S rDNA, molecular evidence suggests the existence of the specimen with rearranged genome that indicate genetic variability in native populations of ash weevil species.

Keywords: Curculionidae, Fraxinus species, Genetic diversity, Molecular taxonomy, Phylogeny

Corresponding author: Vladislava Galović University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13d, 21000 Novi Sad, Serbia. Phone: +381 21 540383; Fax: +381 21 540 385; Mob. +381 69 203 8911, E-mail: galovic@uns.ac.rs, vladislava.galovic@gmail.com

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Published in „GENETIKA“ Vol. 51, No.2(2019), pp. 687-699

UDC 575.633.11

https://doi.org/10.2298/GENSR1902687E

Original scientific paper

The genetic VARIATION AND Stability Analysis of Rice Mutant Lines using AMMI Model Under NORMAL AND Drought Stress Conditions

Ali Akbar EBADI^*1, Mohammad Taher HALLAJIAN², Mojtaba KORDROSTAMI¹

1-Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

2- Researcher at Radiation Application Research School, Nuclear Science

and Technology Research Institute (NSTRI)

Abstract

Ebadi Ali Akbar, Hallajian M.T. and M. Kordrostami (2019): The genetic variation and stability analysis of rice mutant lines using AMMI model under normal and drought stress conditions.- Genetika, Vol 51, No.2, 687-699.

This study was conducted to investigate the genotype × environment interaction and stability analysis of 14 mutant lines (with their parental lines). The study was designed for two years, 2015-2016, in two environmental conditions, normal and drought stress, in Rasht. Based on the results of combined analysis of variance, genotype, environment, and genotype × environment effects were significant at 1% probability level. The significance of the environmental effect indicates that the environments were different in terms of genotypes performance. Therefore, the stability analysis was performed with different methods of measuring stability. The results of ANOVA for the additive main effect and multiplicative interaction (AMMI) showed the genotype and environment main effects and genotype × environment effects were significant at 1% probability level. Based on the AMMI results, TM6-230-VE-7-5-1, TM6-230-VE-8-4-1, Khazar, and KM5-200-4-2-1 had a positive and negative interaction, respectively. HM5-300-5-1, HM5-250-12-1, TM6-B-19-2 were selected as the stable genotypes. Stability analysis determined through AMMI stability value (ASV) method showed HM5-250-12-1, Tarom, HM5-250-E-1-1, TM6-B-19-2, Hashemi and HM5-300-5-1 with the least stability value were introduced as the most stable cultivars.

Keywords: AMMI model; Drought; Mutants; Rice; Stability analysis; G×E interaction

Corresponding author: Ali Akbar Ebadi, Rice Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. E-mail: a.ebadi@areeo.ac.ir.

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 701-715

Serbia

UDC 575.

https://doi.org/10.2298/GENSR1902701J

Original scientific paper

ASSOCIATION OF TGF-β1 AND TNF-α GENES POLYMORPHISMS WITH THE

KIDNEY SCARS FORMING RISK IN CHILDREN WITH

VESICOURETERAL REFLUX

Dragana JUGOVIĆ^1*, Predrag MILJKOVIĆ¹, Tatjana JEVTOVIĆ-STOIMENOV², Milena DESPOTOVIĆ², Višnja MADIĆ³, Aleksandar MILIĆEVIĆ⁴, Marija VUKELIĆ-NIKOLIĆ², Perica VASILJEVIĆ³

¹Pediatric Internal Diseases Clinic, Clinical Center, Niš, Serbia

² University of Niš , Faculty of Medicine, Research Center for Biomedicine, Niš, Serbia

³University of Niš, Faculty of Science and Mathematics, Department of Biology and Ecology, Niš, Serbia

⁴Pathology and Pathological Anatomy Center, Clinical Center, Niš, Serbia

Abstract

Jugović D., P. Miljković, T. Jevtović-Stoimenov, M. Despotović, V. Madić, A. Milićević, M. Vukelić-Nikolić, P. Vasiljević (2019): Association of TGF-Β1 and TNF-Α genes polymorphisms with the kidney scars forming risk in children with vesicoureteral reflux.- Genetika, Vol 51, No.2, 701-715.

Matrix accumulation in the tissue is the main pathological feature of fibrosis. TGF-β1 stimulates the production of extracellular matrix protein and induces fibrosis in different tissues. TNF-α is a proinflammatory cytokine produced in the kidneys by proximal tubular cells, mesenchymal cells, interstitial fibroblasts and macrophages. The aim of this study was to investigate the association of TGF-β1 and TNF-α genes polymorphisms with the risk of developing kidney scars in children with vesicoureteral reflux (VUR). DNA samples analyzed in this study were extracted by a phenol-chloroform method from the peripheral blood of 50 children with VUR and 70 healthy controls. The genotyping was performed by the PCR/RFLP method. Results of this study have shown that homozygous genotype T/T is more frequent in the patient group (χ2 = 13.92, p = 0.0009). It can be concluded that the presence of the genotype T/T on the position -509 in the promoter region TGF-β1 gene is a risk factor for the development of kidney scars in children with VUR (TT vs. CT + CC: OR = 19.4615; CI 2.420-156.477; p = 0.0003). However, there was the absence of association of the promoter region polymorphism of the TNF-α gene region with the risk of developing kidney scars of children with VUR.

Keywords: gene polymorphism, PCR, TNF-α, TGF-β1, VUR, kidney scars

Corresponding author: Dragana Jugović, Pediatric Clinic, Clinical Center of Niš, Bulevar Dr Zorana Đinđića 48, 18000 Niš, Serbia, Tel.: +381 603450233, Email address: dragana.jugovic@live.com

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Published in „GENETIKA“ Vol. 51, No. 2(2019), pp. 717-739

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902717M

Original scientific paper

POPULATION GENETIC STRUCTURE IN Plantago ovata var. decumbens WITH USING ISSR MARKERS

Saeed MOHSENZADEH, Masoud SHEIDAI*, Fahimeh KOOHDAR

Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract

Mohsenzadeh S., M. Sheidai, F. Koohdar (2019): Population genetic structure in Plantago ovata var. decumbens with using ISSR markers.- Genetika, Vol 51, No.2, 717-730.

Plantago ovata (Plantaginaceae) is a medicinal plant cultivated extensively in Western Asia for seed husk known as blonde Psyllium or Isabgol. We used ISSR molecular markers to investigate the population genetic structure and genetic divergence within P. ovata populations. We identified P. ovata variety as var. decumbens based on the morphological data. WARD tree and PCoA plot produced similar results on morphological data. AMOVA revealed a significant genetic differentiation among the studied populations. STRUCTURE analyses showed some degree of gene flow among the studied populations. NJ tree and PCoA plot of ISSR data revealed that there are at least three genetic groups within the studied populations. Though the studied populations in P. ovata are genetically differentiated, there are not qualitative morphological features for differentiating among theirs. Therefore, we consider them as ecotypes within P. ovata.

Keywords: Ecotype, ISSR, Plantago ovata, STRUCTURE analysis

Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Blvd Student, Shahid Shahriari Square, Yemen street, Shahid Chamran highway, , Tel/Fax: +98 2129902111, e-mail: msheidai@yahoo.com

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Published in „GENETIKA“ Vol. 51 No. 2(2019), pp. 731-742

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902731Y

Review paper

GROWTH CHARACTERISTICS OF Tilia tomentosa Moench. FROM DIFFERENT DISTRICTS IN THE REGIONS OF MARMARA AND WESTERN BLACK SEA IN TURKEY

Cengiz YÜCEDAĞ¹, Halil Barış ÖZEL², Sezgin AYAN³*, Fulvio DUCCI⁴, Vasilije V. ISAJEV⁵, Muhidin ŠEHO⁶

¹Department of Landscape Architecture, Mehmet Akif Ersoy University, Burdur, Turkey

²Department of Forest Engineering, Bartın University, Bartın, Turkey

³Department of Silviculture, Faculty of Forestry, Kastamonu University, Kastamonu, Turkey

⁴Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria - Forestry and Wood Research Centre (CREA FL), Arezzo, Italy.

⁵Institute for Forestry, Belgrade, Serbia

⁶Bavarian Office for Forest Seeding and Planting (ASP), Teisendorf, Germany.

Abstract

Yücedağ C., H. B. Özel, S. Ayan, F. Ducci, V. Isajev, M. Šeho (2019): Growth characteristics of Tilia tomentosa Moench. from different districts in the regions of Marmara and Western Black Sea in Turkey - Genetika, Vol 51, No.2, 731-742.

Climate change will affect all ecosystem services of green areas in the districts. In many cases, the tree species in parks and districts are suffering from the increasingly warmer and drier summers and newly immigrated pests and diseases. Tilia tomentosa Moench. is considered as an important tree species under climate change for dry and warm conditions in Central and Southeastern Europe countries. This tree species is often used as valued urban tree. In the present study we investigate the growth patterns of T. tomentosa Moench. in relation to their growing conditions in order to evaluate its interactions in urban contexts. All the studied characters varied significantly among the sampled districts. Within each of the regions, number of flowers showed a wide range 72-104 per m² in Marmara region and 54-124 per m² in Western Black Sea region. All studied characters, beyond number of flowers, positively correlated with each other. This study revealed that especially fruit length had a strong and positive correlation with altitude and a high negative correlation with temperature. Further researches are required to quantify more growth flower characters of the species from Turkey.

Keywords: Flower, growth, Tilia tomentosa, climate change, Urban forestry, Turkey.

Corresponding author: Sezgin Ayan, Department of Silviculture, Faculty of Forestry, Kastamonu University, Kastamonu, Turkey, E-mail: sezginayan@gmail.com

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Published in „GENETIKA“ Vol. 51, No.2(2019), pp. 743-756

Serbia

UDC 575.633.15

https://doi.org/10.2298/GENSR1902743R

Orginal scientific paper

IMPACT OF DROUGHT AND SALT STRESS ON SEED GERMINATION

AND SEEDLING GROWTH OF MAIZE HYBRIDS

Velimir RADIĆ¹, Igor BALALIĆ¹, Goran JAĆIMOVIĆ²,

Aleksandra NASTASIĆ¹, Jasna SAVIĆ³, Ana MARJANOVIĆ-JEROMELA¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia,

²Faculty of Agriculture, University of Novi Sad

³Faculty of Agriculture, Zemun, University of Belgrade

Abstract

Radić V., I. Balalić, G. Jaćimović, A. Nastasić, J. Savić, Ana Marjanović-Jeromela (2019): Impact of drought and salt stress on seed germination and seedling growth of maize hybrids. - Genetika, Vol 51, No.2, 743-756.

Since maize is grown in climatically diverse regions and under different production conditions, the aim of our study was to investigate the effect of two common stress factors (drought and salinity), on seed germination, as well as on seedling root and shoot length of maize hybrids. The experiments were conducted in 2015, in the Laboratory for Seed Testing of the Institute of Field and Vegetable Crops from Novi Sad, Serbia, on seven maize hybrids from different maturity groups (from FAO 300 to FAO 700). For simulation of drought conditions we have used polyethylene glycol (PEG) 6000 (control and concentration of 1, 10, 16 and 23%). For study of salt stress, NaCl in concentration of 0.02, 0.07, 0.12, 0.17, 0.20 and 0.22 M has been used. The data obtained were processed by ANOVA. Duncan’s Multiple Range Test was used to measure statistical differences between treatment methods and the control. In drought stress study, germination percentage started to decrease at the 0.1 MPa. Root and seedling length were less affected by PEG treatment. In salt stress study, a significant drop in germination was observed at the concentration of 0.20 M of NaCl.

Keywords: maize, seed germination, stress, drought, salinity

Corresponding author: Velimir Radić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad 21000, Serbia, email: velimir.radic@nsseme.com

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Published in „GENETIKA“Vol. 51, No.2(2019), pp. 757-768

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR1902757T

Orginal scientific paper

YIELD AND CONTENT OF NUTRIENT ELEMENTS IN VARIOUS CULTIVARS

OF LETTUCE DEPENDING FROM PRODUCTION METHOD

Ivana TOŠIĆ¹, Milan MIROSAVLJEVIĆ², Novo PRŽULJ³, Miro STOŠIĆ⁴

¹Agricultural Institute of Republic of Srpska, Banja Luka, Republic of Srpska, B&H

²Institute of Field and Vegetable crops, Novi Sad, Serbia

³University of Banja Luka, Faculty of Agriculture, Republic of Srpska, B&H

⁴University Josip Juraj Strossmayer, Faculty of Agrobiotehnical Sciences, Osijek, Croatia

Abstract

Tošić I., M. Mirosavljević, N. Pržulj, M. Stošić (2019): Yield and content of nutrient elements in various cultivars of lettuce depending from production method.- Genetika, Vol 51, No2., 757-768.

In order to examine the influence of cultivars and to estimate how mulching and plant covering with agrotextile affects the content of P, Ca, K, Mg and Mn in lettuce leaves this three-year experiment has been established. In the experiment, black and white plastic foil were used for mulching prior to planting, and agrotextile for plant covering after planting. The effect of each of them as well as their combination on the content of nutrients and yield of lettuce leaves was examined. There were six treatments in the experiment: control, black foil, white foil, black foil and agrotextile, white foil and agrotextile, and agrotextile. Mulching had a significant impact on yield, as well as the content of P, Ca, K, Mg and Mn in lettuce leaves in the three-year experiment. The highest yield was registered in the cultivar Nizzi in treatment with black plastic foil, compared to all cultivars and treatments. The largest P content was registered in the cultivar Sunstar (0.71%), while the smallest content was registered in the cultivar Sunny (0.59%). The Ca content ranged from 2.59% (Sunny) to 2.84% (Nizzi). The Mg content ranged from 0.46% in treatment with black foil to 0.70% in treatment with white foil. The content of Mn in lettuce leaves ranged from 60.99 mg kg^-1 in the cultivar Zeralda to 81.79 mg kg^-1 in the cultivar Devonia.

Keywords: cultivars, Lactuca sativa L., macroelements, mulching, yield

Corresponding author: Ivana Tošić, Agricultural Institute of Republic of Srpska, Banja Luka, Knjaza Miloša 17, 78000, Banja Luka, Republic of Srpska, Bosnia and Herzegovina, it.tosic@gmail.com

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Published in „GENETIKA“Vol. 51, No.2(2019), pp. 769-769

Serbia
https://doi.org/10.2298/GENSR1902769B

Corrigendum: Batos B., D. Miljković (2019): The phenotypic plasticity of of Picea omorika /panč./purkyne morphological pollen traits.- Genetika, Vol 51, No.1, 121-136. https://doi.org/10.2298/GENSR1901121B

Error on page 127 in Fig.3, temparature data during the years of pollen collection (1991 and 1992) – Hydrometeorological station Zlatibor (northern latitude 43º 44'; eastern longitude 19º 43') (www.hidmet.gov.rs), is missed.

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