GENETIKA, Vol. 52, No. 3 (2020)
Emre SEVINDIK, Sinem AYDIN, Mehmet Yavuz PAKSOY, Bahar Bilgin SOKMEN
ANTI-UREASE, TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITIES OF SOME Inula L. (ASTERACEAE) TAXA IN TURKEY [Abstract] [Full text]
Reza ATAEI, Mohammad Reza SHIRI
MULTI-ENVIRONMENT EVALUATION OF FOXTAIL MILLET ADVANCED LINES FOR FORAGE YIELD STABILITY [Abstract] [Full text]
Martyna BIENIEK-KOBUSZEWSKA, Grzegorz PANASIEWICZ, Bożena SZAFRANSK
IDENTIFICATION OF 1093-BP INTRON A, SNPS AND INDELS
DISCOVERED IN THE PORCINE PREGNANCY-ASSOCIATED GLYCOPROTEIN 2-LIKE (PPAG2-L)
GENE SUBFAMILY IN PIGS [Abstract] [Full
text]
Yalda ARGHAVANIAN, Mina ADAMPOUR, Nasser POULADI Nazanin BAGHERLOU, Mohammad Ali Hosseinpour FEIZI, Narges DASTMALCHI, Esmaeil BABAEI
THE SINGLE NUCLEOTIDE POLYMORPHISM ARG399GLN RS25487 IN XRCC1 GENE IS A BREAST CANCER RISK FACTOR, BUT IS NOT RELATED TO TP53 MUTATION STATUS[Abstract] [Full text]
Tünde JURÍKOVÁ, Jiří MLČEK, Marcela ŽITNÁ, Irena HLAVÁČOVÁ, Libor DOKOUPIL, Jiří SOCHOR, Sezai ERCISLI, Gursel OZKAN
FRUIT MATURITY STAGE IN RELATION TO CONTENT OF POLYPHENOLS, FLAVONOIDS AND ANTIOXIDANT ACTIVITY OF SELECTED CLONES OF Lonicera kamtschatica (Sevast.) Pojark [Abstract] [Full text]
Mahmood ASLAN-PARVIZ, Mansoor OMIDI, Varahram RASHIDI, Alireza ETMINAN, Alireza AHMADZADEH
EVALUATION OF GENETIC DIVERSITY OF DURUM WHEAT (Triticum
durum Desf.) GENOTYPES USING INTER-SIMPLE SEQUENCE REPEAT (ISSR) AND CAAT
BOX-DERIVED POLYMORPHISM (CBDP) MARKERS [Abstract] [Full
text]
Nemanja ĆUK, Sandra CVEJIĆ, Velimir MLADENOV, Brankica BABEC, Vladimir MIKLIČ, Dragana MILADINOVIĆ, Siniša JOCIĆ
VARIABILITY OF MORPHOLOGICAL TRAITS IN SUNFLOWER INBRED LINES [Abstract] [Full text]
Špela MESTINŠEK MUBI, Sinja SVETIK, Marko FLAJŠMAN, Jana MUROVEC
In vitro TISSUE CULTURE AND GENETIC ANALYSIS OF TWO HIGH-CBD MEDICAL CANNABIS (Cannabis sativa L.) BREEDING LINES [Abstract] [Full text]
GENETIC VARIABILITY AND POPULATION STRUCTURE OF SAUDI ARABIA BREAD WHEAT (Triticum aestivum L.) BY MICROSATELLITE MARKERS [Abstract] [Full text]
Erdem SIMSEK, Derya KILIC, Oguzhan CALISKAN
PHENOTYPIC VARIATION OF FIG GENOTYPES (Ficus carica L.) IN THE EASTERN MEDITERRANEAN OF TURKEY [Abstract] [Full text]
Jamil M. HASAN, Umma M. KULSUM, Rani R. MAJUMDER, Umakanta SARKER
GENOTYPIC VARIABILITY FOR GRAIN QUALITY ATTRIBUTES IN RESTORER LINES OF HYBRID RICE [Abstract] [Full text]
Swarajyalakshmi N. BOLLINENI, Bukya J. NAIK, Suresh E. NAIK, Gopalakrishnamurthy KADAMBARI, G. Eswar REDDY, S. MALATHI, Eswar N.P. REDDY, Srividhya AKKAREDDY, Reddi M. SEKHAR, Bahben TANTI, B. Krishna VENI, B. Vijaya LAKSHMI, Lakshminarayana R. VEMIREDDY
GENETIC DIVERSITY, POPULATION STRUCTURE AND ALLELE MINING OF GENES GOVERNING GRAIN SIZE RELATED TRAITS IN RICE (Oryza sativa L.) [Abstract] [Full text] [Supplement]
Slavko BRANKOVIC, Dejan NIKOLIC, Dragoslav MARINKOVIC, Suzana CVJETICANIN
GENETIC HOMOZYGOSITY AS A MARKER OF SMOKING DEPENDENCY [Abstract] [Full text]
Mohammad Reza NAROUI RAD, Gholamali KEYKHA, Jahangir ABBASKOOHPAYEGANI, Ramin RAFEZI
MACHINE LEARNING APPROACHES TO CLASSIFY MELON LANDRACES BASED ON PHENOTYPIC TRAITS [Abstract] [Full text]
Sara KARAMI, Aras RAFIEE
THE POSSIBILITY OF BENOMYL AND DIAZINON PESTICIDE’S CARCINOGENICITY AND THE POTENTIAL OF HOTAIR AND H19 AS A SERUM BIOMARKER IN BREAST CANCER[Abstract] [Full text]
Firdes ULAS, Halit YETISIR Abdullah ULAS
EFFECTS OF GRAFTING ON FRUIT YIELD AND LEAF NUTRIENT CONTENTS OF PEPPER (Capsicum annuum L.) INBRED LINES [Abstract] [Full text]
Vera POPOVIĆ, Savo VUČKOVIĆ, Zoran JOVOVIĆ, Nikola RAKAŠČAN, Marko KOSTIĆ, Nataša LJUBIČIĆ, Milena MLADENOVIĆ-GLAMOČLIJA, Jela IKANOVIĆ
GENOTYPE BY YEAR INTERACTION EFFECTS ON SOYBEAN MORPHO-PRODUCTIVE TRAITS AND BIOGAS PRODUCTION [Abstract] [Full text]
Fatemeh NAJARIYAN, Masoud SHEIDAI, Fahimeh KOOHDAR, Seyed Mehdi TALEBI
MOLECULAR PHYLOGENY OF THE SECT. ADONIS (Genus Adonis L., Ranunculaceae) [Abstract] [Full text]
Mona Pishnamazzadeh EMAMI, Nasser MOHEBALIPOUR, Ali Akbar EBADI, Hassan NOURAFCAN, Jalil AJALI
QTL MAPPING OF SOME MORPHOLOGICAL TRAITS OF RICE IN A
RILS POPULATION DERIVED FROM HASHEMI AND MODIFIED NEMAT VARIETIES HYBRID [Abstract] [Full
text]
Mohamed N. BARAKAT, Abdullah A. Al-DOSS, Khaled A. MOUSTAFA, Mohamed I.
MOTAWEI, Ibrahim M. AL-ASHKAR, Soleman M. AL-OTAYK, Mohamed S. ALAMRI, Mohamed
MERGOUM
MAPPING WHEAT QTLS FOR GRAIN YIELD RELATED TRAITS
UNDER HIGH TEMPERATURE STRESS [Abstract] [Full
text]
Yaocheng JIA, Majid KHAYATNEZHAD, Shahram MEHRI
POPULATION DIFFERENTIATION AND GENE FLOW IN Erodium cicutarium: A POTENTIAL MEDICINAL PLANT [Abstract] [Full text]
Borislav BANJAC, Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Velimir MLADENOV1 Dušana BANJAC, Biljana KIPROVSKI
Antioxidant variability of wheat genotypes under salinity stress in situ [Abstract] [Full text]
Kaushik Kumar PANIGRAHI, Puranjaya PANIGRAHI, Ayesha MOHANTY, Purandar MANDAL, Basudeba SATAPATHY
DEVELOPMENT OF NEW MICROSATELLITE MARKERS FOR DNA
FINGERPRINTING PATTERN OF BLACK GRAM (Vigna mungo L. Hepper) AND GREEN
GRAM (Vigna radiate L. Wilckzek) [Abstract] [Full
text] [Supplement]
Amir POURBORHANZADEH, Masoud SHEIDAI, Zahra NOORMOHAMMADI, Naser KALHOR, Mehdi Heidari HORESTAN
ASSOCIATION STUDY OF MTHFR-C677T WITH MALE INFERTILITY AND REPORTING NEW POTENTIAL SNPS/ SEQUENCE VARIANTS AS A SOURSE OF POPULATION GENETIC MARKERS [Abstract] [Full text]
Biljana MILIĆ, Biljana ERDELJAN, Tanja JANKOVIĆ, Milica POPOVIĆ, Gordana STRAŽMEŠTER-MAJSTOROVIĆ, Katarina ILIĆ
PHENOTYPE DIFFERENCES IN HLA-B27 POSITIVE VERSUS NEGATIVE PATIENTS WITH ANKYLOSING SPONDYLITIS TREATED WITH TUMOR NECROSIS FACTOR ALPHA INHIBITORS [Abstract] [Full text]
Marijenka TABAKOVIĆ, Mile SEČANSKI, Rade STANISAVLJEVIĆ, Snežana MLADENOVIĆ DRINIĆ, Milena SIMIĆ, Jasmina KNEŽEVIĆ, Violeta ORO
THE IMPACT OF
AGROECOLOGICAL FACTORS ON MORPHOLOGICAL TRAITS OF MAIZE [Abstract] [Full
text]
Rima KUMARI, Pankaj KUMAR, V.K. SHARMA, Harsh KUMAR
GENOME PROFILING OF DIFFERENTIAL SALT STRESS RESPONSIVE LANDRACES AND VARIETIES OF RICE USING ISSR MARKERS [Abstract] [Full text]
Vladan POPOVIĆ, Aleksandar LUČIĆ, Ljubinko RAKONJAC, Zoran MAKSIMOVIĆ, Danijela RISTIĆ
VARIABILITY OF MORPHOLOGICAL AND
ANATOMICAL CHARACTERISTICS OF SERBIAN SPRUCE (Picea omorika /PANČ./
PURKYNE) NEEDLES OF NATURAL POPULATION LOCATED IN THE MILIEŠEVKA RIVER CANYON [Abstract] [Full
text]
Mirjana SREBRIĆ, Zoran DUMANOVIĆ, Vesna PERIĆ, Violeta ANDJELKOVIĆ
DECREASE OF YIELD COMPONENTS AND MORPHOLOGICAL TRAITS OF SOYBEAN FULL-SIBS UNDER DROUGHT CONDITIONS [Abstract] [Full text]
INFLUENCE OF DIFFERENT ENVIRONMENTAL CONDITIONS AND GIBERELLIC ACID TREATMENT ON FLOWERING TIME OF DIVERGENT GENOTYPES OF CABBAGE (Brassica oleracea var. capitata L.) AND THEIR F1 HYBRIDS [Abstract] [Full text]
Zeshan Haider RAZA, Asif NADEEM, Maryam JAVED, Faiz-ul-HASSAN, Wasim SHEHZAD, Arfan AHMAD, Muhammad SAADULLAH, Muhammad Muddassir ALI
POLYMORPHIC STATUS AND PHYLOGENETIC ANALYSIS OF MYOSTATIN GENE IN PAK-THOROUGHBRED [Abstract] [Full text]
Published in „GENETIKA“ Vol. 52, No.3 (2020), pp. 825-834
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.630
https://doi.org/10.2298/GENSR2003825S
Orginal scientific article
ANTI-UREASE, TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITIES OF SOME Inula L. (ASTERACEAE) TAXA IN TURKEY
Emre SEVINDIK1, Sinem AYDIN2*, Mehmet Yavuz PAKSOY3, Bahar Bilgin SOKMEN4
1Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, South Campus, Cakmar, Aydin, Turkey
2Giresun University, Faculty of Science and Arts, Department of Biology, Giresun, Turkey
3Munzur University, Tunceli Vocational School,Department of Medical Services and Techniques, Medical Documentation and Secretaryship Pr. Tunceli, Turkey
4Giresun University, Faculty of Science and Arts, Department of Chemistry, Giresun, Turkey
Abstract
Sevindik E., S. Aydın, M. Yavuz Paksoy, B. Bilgin Sökmen (2020). Anti-urease, total phenolic content and antioxidant activities of some Inula L. (Asteraceae) taxa in Turkey. - Genetika, Vol 52, No.3, 825 - 834.
The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54±0.002 µg GAE/mL) and I. macrocephala (189.16±0.002 µg AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.
Keywords: Inula, anti-urease, antioxidant, total phenolic, medicinal plant.
Corresponding author: Sinem Aydın, Giresun University, Faculty of Science and Arts, Department of Biology, Giresun, Turkey, E-mail : sinem.aydin@giresun.edu.tr
Published in „GENETIKA“ Vol. 52, No. 3 (2020), pp.835-850
© 2020Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003835A
Original scientific paper
MULTI-ENVIRONMENT EVALUATION OF FOXTAIL MILLET ADVANCED LINES FOR FORAGE YIELD STABILITY
Reza ATAEI, Mohammad Reza SHIRI
Seed and Plant Improvement Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran.
Abstract
Ataei R. and M. Reza Shiri (2020). Multi-environment evaluation of foxtail millet advanced lines for forage yield stability. - Genetika, Vol 52, No.3, 835 - 850.
Foxtail millet (Setaria italica L. P. Beauv.) is a very important crop in the arid and semi-arid areas. In the present study, five advanced lines and one commercial check (Bastan) were tested across12 (six locations and two years) environments. An attempt was made to identify superior line(s) with emphasis on high forage yield potential and yield stability over multi-environment trials using GGE biplot analysis. The study was conducted using a randomized complete block design with four replications. Combined analysis of variance for forage yield showed that the genotypes, environments, and the interaction effects were highly significant (P ≤ 0.01). The environment, genotype and interaction effects accounted for 76.38%, 6.97% and 8.92% of the total forage yield variation, respectively. GGE biplot analysis showed that G5 has both high forage yield and stability across the studied environments and E3 and E4 were high-yielding environments in this study. Which-won-where study partitioned the testing locations into two mega-environments with G5 and G3 as winning genotypes in the first and second mega-environment, respectively. According to discriminate ability and representativeness, the E4 and E12 environments were perfect environments. Overall, existence of extensive crossover interaction clearly suggested that efforts are necessary to identify location-specific genotypes over multi-environment trials for release of new varieties.
Keywords: Cross over interaction, Forage yield, Stability analysis, Foxtail millet, GGE biplot
Corresponding author: Reza Ataei, Seed and Plant Improvement Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. E-Mail: reza_ataei@ut.ac.ir, Tell: +989193555327
Published in „GENETIKA“ Vol. 52, No. 3 (2020), pp.851-866
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003851B
Original scientific paper
Martyna BIENIEK-KOBUSZEWSKA1,#, Grzegorz PANASIEWICZ2,#,*,
Bożena SZAFRANSKA2
1Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
2Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
# The first two authors contributed equally to the work described in this manuscript.
Abstract
Bieniek-Kobuszewska M., G. Panasiewicz, B. Szafranska (2020). Identification of 1093-bp intron a, SNPS and indels discovered in the porcine pregnancy-associated glycoprotein 2-like (PPAG2-l) gene subfamily in pigs - Genetika, Vol 52, No.3, 851-866.
The objective of this study was to identify the intron A sequence (between exons 1 and 2) of pPAG2-L, novel single nucleotide polymorphisms (SNPs) and mutations (InDels) within intron A in the crossbreed (Landrace x Large White), Hirshmann hybrid and Duroc pigs. Genomic templates were isolated from leukocytes, amplified, and the gel-out were purified and then sequenced. Positive amplification control included CH242-60C13 BAC clone (Duroc) containing pPAG1-L and pPAG2-L. This is the first report that describes the 1093-bp intron A sequence from the pPAG2-L (Acc. No. KF471015; GenBank), which increased general knowledge of the porcine genome. Novel SNPs/InDels were identified within the intron A of the pPAG2-L in the crossbreeds (72), Duroc (45) and Hirshmann hybrids (17). This is a pioneer study describing identification of the intron A and SNPs/InDels in crossbreeds that provides a novel major pattern that represents a large portion of the genetic variation within the porcine genome. This information should be valuable when genotyping (coding and noncoding regions) multiparous sows from many breeds, in which reproductive phenotypes are known.
Keywords: intron A, porcine PAG2-L, SNP, insertion, deletion.
Corresponding author: Grzegorz Panasiewicz, Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10–719 Olsztyn, Poland; E-mail panasg@uwm.edu.pl
tel. +48 89 523 42 33
Published in „GENETIKA“ Vol. 52, No3(2020), pp. 867-879
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
. https://doi.org/10.2298/GENSR2003867A
Original scientific paper
THE SINGLE NUCLEOTIDE POLYMORPHISM ARG399GLN RS25487 IN XRCC1 GENE IS A BREAST CANCER RISK FACTOR, BUT IS NOT RELATED TO TP53 MUTATION STATUS
Yalda ARGHAVANIAN1, Mina ADAMPOUR1, Nasser POULADI2, Nazanin BAGHERLOU3, Mohammad Ali Hosseinpour FEIZI1, Narges DASTMALCHI1, Esmaeil BABAEI1
1Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
2Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran
3 Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.
Abstract
Arghavanian Y., M. Adampour, N. Pouladi, N. Bagherlou, M. A. H. Feizi, N. Dastmalchi, E. Babaei (2020). The single nucleotide polymorphism arg399gln rs25487 in XRCC1 gene is a breast cancer risk factor but is not related to tp53 mutation status - Genetika, Vol 52, No.3, 867-879.
Genetic changes in DNA repair genes, such as X-ray cross-complementing group1 (XRCC1), can cause modifications in the capacity of damaged DNA repair and affect the risk of cancer. Several mutations in TP53, which is a tumor suppressor gene, have been associated with breast cancer. In this study, it is aimed to evaluate the association of genetic variation in XRCC1rs25487 single nucleotide polymorphism (SNP) with TP53 mutation and breast cancer risk. In this research, 200 breast cancer women and 200 controls from the Iranian-Azeri population, Iran, were enrolled. Genomic DNA was extracted from the whole blood of controls patients. PCR-RFLP was carried out to genotype all participants for XRCC1rs25487SNP. Determination of possible mutations of TP53 in exons 5,6,7, and 8 were performed on 30 cancerous breast tissues through sequencing the amplified fragments. Our results of the case-control study indicated that the GA genotype of XRCC1 gene in rs25487polymorphism has a significantly risk effect on the breast cancer in the dominant genetic model (OR: 1.580, 95% CI: (1.025-2.436); p-value =0.049) and also GA genotype of XRCC1 gene in rs25487polymorphism has a protective effect on breast cancer in overdominant genetic model (OR: 0.591, 95% CI (0.395-0.886), p-value = 0.014). Furthermore, genotypes of this SNP did not associate with the clinical specifications of the patients and P53 mutation status. Sequencing results showed the mutational spectrum of P53 in the studied cases. According to the results of this study, in some of the genetic models, XRCC1SNP appears to be a modulator of breast cancer risk in Iranian East-Azerbaijan women. However, there was no correlation between XRCC1SNP and TP53 mutation status of the tumor.
Keywords: Breast cancer, mutation, polymorphism, TP53, XRCC1
Corresponding author: Mohammad Ali Hosseinpour Feizi, Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. E-mail: nasserpouladi52@gmail.com; pourfeizi@eastp.ir. Telephone: +984133370497
Published in „GENETIKA“ Vol. 52, No.3 (2020), pp. 881-893
© 2020 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003881J
Original scientific paper
FRUIT MATURITY STAGE IN RELATION TO CONTENT OF POLYPHENOLS, FLAVONOIDS AND ANTIOXIDANT ACTIVITY OF SELECTED CLONES OF Lonicera kamtschatica (Sevast.) Pojark
Tünde JURÍKOVÁ1, Jiří MLČEK2*, Marcela ŽITNÁ3, Irena HLAVÁČOVÁ2,
Libor DOKOUPIL4, Jiří SOCHOR5, Sezai ERCISLI6, Gursel OZKAN6
1Institute for Teacher Training, Faculty of Central European Studies, Constantine The Philosopher University in Nitra, Nitra, Slovak Republic.
2Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, Zlín, Czech Republic
3Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University in Nitra, Nitra, Slovak Republic
4Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic
5Department of Viticulture and Enology, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic
6Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
Abstract
Juríková T., J. Mlček, M. Žitná, I. Hlaváčová, L. Dokoupil, J. Sochor, S. Ercisli, G. Ozkan (2020). Fruit maturity stage in relation to content of polyphenols, flavonoids and antioxidant activity of selected clones of Lonicera kamtschatica (sevast.) Pojark- Genetika, Vol 52, No.3, 881–893.
The article is dealing with evaluation of four fruit maturity stages of 6 selected clones (LKL-3, LKL-14, LKL-19, LKL-33, LKL-96 and LKL-103) of Lonicera kamtschatica (Sevast.) Pojark in relation to total polyphenols, flavonoids content and antioxidant activity of berries. The experiment in 5 replications of each clones has been established in the conditions of Botanical Garden in Nitra (Slovak republic)in 2016. The results of analyses proved that in respect of the total polyphenols content (TPC), the content of flavonoids (FC) and the antioxidant activity the most valuable clones at the stage of full ripened fruit were following: LKL-14 (8.77; 2.57 g/kg FW; 9.07 g/kg), LKL-33 (6.77; 2.84 g/kg FW; 9.16 g/kg) and LKL -3 (8.19; 2.61 g/kg FW; 8.94 g/kg). The results of statistical analyses by one-way analysis of variance proved significant differences among assayed clones in total flavonoids and antioxidant activity of fruit but no differences among content of total polyphenols and assayed stages of fruit maturity.
Keywords: Lonicera, antioxidants, polyphenols, fruits
Corresponding author: Jiří Mlček, Department of Food Analysis and Chemistry, Faculty of Technology, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic mlcek@utb.cz; Tel.: +420 576 033 030
Published in „GENETIKA“ Vol. 52, No. 3 (2020), pp. 895-909
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2003895A
Original scientific paper
Mahmood ASLAN-PARVIZ1, Mansoor OMIDI2*, Varahram RASHIDI1, Alireza ETMINAN3, Alireza AHMADZADEH4
1Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran
3Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
4Department of Plant Breeding and Biotechnology, Shabestar Branch, Islamic Azad University, West Azerbaijan, Iran.
Abstract
Aslan-Parviz M., M. Omidi, V. Rashidi, A. Etminan, A. Ahmadzadeh (2020). Evaluation of genetic diversity of durum wheat (Triticum durum desf.) genotypes using inter-simple sequence repeat (ISSR) and caat box-derived polymorphism (CBDP) markers. - Genetika, Vol 52, No.3, 895-909.
Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several CAAT box-derived polymorphism (CBDP) and inter-simple sequence repeat (ISSR) markers. Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively. CBDP primers showed the higher mean values for informativeness parameters such as polymorphic information content (PIC), resolving power (Rp) and marker index (MI) in comparison with ISSR primers. The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred within populations. Furthermore, CBDP primers indicated high values for all genetic parameters. Besides, the highest values of genetic parameters including number of observed (Na) and effective alleles (Ne), Shannon’s information index (I) and Nei’s gene diversity (He) were estimated for Iranian durum wheat landraces. Cluster analysis based on each molecular technique classified all durum wheat genotypes into three main groups, so that the results of principal coordinate analysis (PCoA) supported the grouping patterns. As a result, the grouping pattern observed by ISSR primers was clearer than CBDP primers and grouped all samples based on their origins. However, Mantel’s coefficient correlation test illustrated the higher positive correlation (0.54) between both marker techniques. Hence, the use of these markers in combination with each other to evaluate the genetic diversity is recommended.
Keywords: Genetic variability, DNA-based markers, PCoA, molecular analysis of variance, durum wheat
Corresponding author: Mansoor Omidi, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran, E-mail: momidi@ut.ac.ir , Telephone number: (+98) 912 564 4435
Published in „GENETIKA“ Vol. 52, No.3(2020), pp.911-923
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003911C
Original scientific paper
VARIABILITY OF MORPHOLOGICAL TRAITS IN SUNFLOWER INBRED LINES
Nemanja ĆUK1, Sandra CVEJIĆ1, Velimir MLADENOV2,
Brankica BABEC1, Vladimir MIKLIČ1, Dragana MILADINOVIĆ1, Siniša JOCIĆ1
1Institute of Field and Vegetable Crops, Novi Sad, Serbia,
2Faculty of Agriculture, University of Novi Sad, , Novi Sad, Serbia
Abstract
Ćuk N., S. Cvejić, V. Mladenov, B. Babec, V. Miklič, D. Miladinović, S. Jocić (2020). Variability of morphological traits in sunflower inbred lines - Genetika, Vol 52, No.3, 911-923.
Except agronomic important traits, great diversity in sunflower is present in morphological traits which are very useful in breeding studies. The main objective of the paper was to determine genetic diversity among the 110 inbred lines in the collection of Institute of Field and Vegetable Crops Novi Sad (IFVCNS) by screening 34 morphological traits according to a list of descriptors of the International Union for the Protection of New Varieties of Plants (UPOV) as to conduct the Distinctness, Uniformity and Stability Test (DUS). The diversity of morphological traits was estimated by Shannon diversity index (H’) and the diversity of sunflower inbred lines was performed by homogeneity analysis (HOMALS) as well as discriminatory power of the traits. The values of the traits in Shannon diversity index were the highest (H’=0.99) for height of the tip of the blade compared to insertion of petiole and bract position, while branching, head shape and seed color showed low diversity (H’>0.1). The uniformity of inbred lines distribution determined discriminative power of descriptors. Disk flower anthocyanin coloration of stigma, hypocotyl anthocyanin coloration and intensity, leaf blistering, leaf serration, seed stripes on and between the margins showed the strongest discriminatory power. According to these six traits, the collection of inbred lines was divided into two main groups and three subgroups which better explained the relationships among the various inbred lines. Inbred lines showed the great variability of morphological traits in the whole collection and also among the inbred lines from the same type of use.
Keywords: genetic diversity, inbred lines, sunflower, morphological traits
Corresponding author: Sandra Cvejić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia, sandra.cvejic@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 52, No.3 (2020), pp.925-941
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003925M
Original scientific paper
In vitro TISSUE CULTURE AND GENETIC ANALYSIS OF TWO HIGH-CBD MEDICAL CANNABIS (Cannabis sativa L.) BREEDING LINES
Špela MESTINŠEK MUBI1, Sinja SVETIK1, Marko FLAJŠMAN1, Jana MUROVEC1*
1Department for Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Abstract
Mestinšek Mubi Š., S. Svetik, M. Flajšman, J. Murovec (2020). In vitro tissue culture and genetic analysis of two high-CBD medical cannabis (Cannabis sativa L.) breeding lines - Genetika, Vol 52, No.3, 925-941.
The species Cannabis sativa L. has recently witnessed a resurgence of interest all over the world due to its multipurpose applications and the scientific confirmation of its pharmacological properties. Genotypes with high cannabinoid content are appreciated in the pharmaceutical and cosmetic industries due to their therapeutic potential. These genotypes, with predominantly high cannabidiol (CBD) content, are the subject of research and breeding in several programs, but to date, little data is published on the in vitro tissue culture of cannabis. Our study focused on the establishment of an efficient micropropagation method for two high-CBD breeding lines (MX-CBD-11 and MX-CBD-707) as the basis for advanced biotechnological breeding approaches. The results demonstrated that the in vitro culture of medical cannabis can be initiated on different culture media, that cultured plants can be successfully acclimatized, and that nodal position, and especially the genotype, have a significant influence on the success of shoot culture establishment. They showed that the published tissue culture media optimized for one high-THC strain of Mexican cannabis are not as efficient for other genotypes of (medical) cannabis. We complemented this research with a genetic study of 95 plants of the two breeding lines with 16 microsatellite (SSR) markers which clustered the plants based on breeding line. The results demonstrated that only 8 markers are needed for discrimination of all analyzed plants and their usefulness for clonal identification.
Keywords: Cannabis sativa L., medical cannabis, tissue culture, cannabinoids.
Corresponding author: Jana Murovec, Department for agronomy, Biotechnical faculty, University of Ljubljana, Ljubljana, Slovenia, Phone: +386 (0)1 320 32 65, E-mail: jana.murovec@bf.uni-lj.si
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 943-956
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2003943A
Original scientific paper
Ibrahem A. ALMOHISEN
Department of Biology, College of Science and Humanities, Shaqra University, Qwaieah,
Saudi Arabia
Abstract
Almohisen A. Ibrahem (2020). Genetic variability and population structure of Saudi Arabia bread wheat (Triticum aestivum L.) by microsatellite markers- Genetika, Vol 52, No.3, 943-956.
Wheat (Triticum aestivum L.) is an important cereal crop. Analysis of genetic diversity and population structure in local landraces would improve the wheat breeding program by more efficient use of genetic materials and management of genetic variation. To address this challenge, a set of thirteen Saudi Arabia wheat landraces was used to assess population structure and genetic diversity. Thirteen landraces were genotyped using eighteen microsatellite markers which revealed a clear polymorphism among these genotypes. In total, 136 alleles from a set of eighteen simple sequence repeats (SSRs) loci on a panel of thirteen wheat landraces were detected. All SSRs loci showed a wide range of allele numbers extended from 3 to 11 alleles with an average of 7.5. Genetic diversity, polymorphism information content and minor allele frequency ranged from 0.26 to 0.50, 0.23 to 0.37 and 0.15 to 0.46 with an average 0.43, 0.33 and 0.34, respectively. The results of principal coordinate analysis (PCoA) agreed with the structure analysis. Structure grouped the thirteen landraces into three clear subpopulations. The fixation index (Fst), a measure of population substructure, was 0.217, 0.432 and 0.541 for G2, G1, and G3, respectively. Furthermore, analysis of molecular variance recognized 35% variance among and 65% within populations. The present study showed a high genetic diversity between landraces which can be exploited to produce new bread wheat cultivars.
Keywords: Wheat (Triticum aestivum L), Genetic diversity, Landraces, SSRs, Population structure.
Corresponding author: Ibrahem A. Almohisen, Department of Biology, College of Science and Humanities, Shaqra University, Qwaieah, Saudi Arabia, E-mail: ibraheem@su.edu.sa, Tel: +996 (0)506488462
Published in „GENETIKA“ Vol. 52, No.3 (2020), pp. 957-972
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003957S
Original scientific paper
PHENOTYPIC VARIATION OF FIG GENOTYPES (Ficus carica L.) IN THE EASTERN MEDITERRANEAN OF TURKEY
Erdem SIMSEK, Derya KILIC, Oguzhan CALISKAN*
Hatay Mustafa Kemal University, Faculty of Agriculture, Department of Horticulture,
Hatay, Turkey
Abstract
Simsek E., D. Kilic, O. Caliskan (2020). Phenotypic variation of fig genotypes (Ficus carica L.) in the Eastern Mediterranean of Turkey- Genetika, Vol 52, No.3, 957-972.
Turkey is one of the main genetic origins of figs (Ficus ssp.). The study was carried out to determine the phenotypic variation of fig genotypes grown in Tarsus, Mersin, in the eastern Mediterranean region of Turkey. In the study, 24 female fig genotypes selected from Tarsus were investigated based on plant and fruit quality characteristics. Only Haziran İnciri ripened the breba (first) crop and the other genotypes ripened the main crop. The fruit weight of the fig genotypes ranged between 22.37 g and 90.16 g, total soluble solids content (TSS) ranged between 12.6 and 25.0% and TSS/acidity ranged between 29.2 and 161.5. From the plant and fruit characteristics, 26 out of 45 attributes were determined to be more appropriate in the identification and discrimination of the fig genotypes in the research area. Siyah İncir6, Bardak1, Sarı İncir1 and Siyah İncir3 genotypes were the highest in overall quality parameters. The results showed that the measured plant and fruit characteristics of the fig genotypes have significant phenotypic variations.
Keywords: Ficus carica, genotype, variation, principle component analysis, cluster
Corresponding author: Oguzhan Caliskan, Hatay Mustafa Kemal University, Faculty of Agriculture, Department of Horticulture, Hatay, Turkey, Tel: +903262455845, Fax: +903262455832, e-mail: ocaliskan@mku.edu.tr or caliskanoguzhan@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 973-989
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003973H
Original
scientific paper
GENOTYPIC VARIABILITY FOR GRAIN QUALITY ATTRIBUTES
IN RESTORER LINES OF HYBRID RICE
Jamil M. HASAN1, Umma M. KULSUM1, Rani R. MAJUMDER2 and Umakanta SARKER3*
1 Hybrid Rice Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh
2 Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur-1701, Bangladesh
3Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
Abstract
Hasan M. J., U. M. Kulsum, R. R. Majumder, U. Sarker (2020). Genotypic variability for grain quality attributes in restorer lines of hybrid rice - Genetika, Vol 52, No.3, 973-989.
Twenty-six restorer line were selected for evaluation for genetic variability, heritability and genetic association among physicochemical, nutritional, yield and yield contributing traits in randomized complete block design (RCBD) with three replications. The analysis showed that restorer line has milling rice percentage, head rice recovery percentage, cooking time, elongation ratio, amylase content and protein content with average value among 26 genotypes (64.60%, 73,00%, 16.67 min, 1.38, 19.26% and 6.36% respectively). Highest grain yield was obtained from the restorer line 3038R (15.99 g/plant), followed by BRRI20R (14.83 g/plant), PR828R (14.10 g/plant), PR312R (13.91 g/plant), BRRI31R (13.61 g/plant) and BRRI19R (13.28 g/plant). The line BRRI20R and BRRI31R had high (25.00%) amylase and protein content, milling rice percentage, head rice recovery percentage. F2277R had less (14.00 min) cooking time. Grain yield exhibited a strong positive correlation with head rice recovery percentage, amylase content and thousand grain weight. Insignificant genotypic correlation was observed among physicochemical, nutritional, yield related traits and grain yield. Most of the studied traits indicating that selection with these traits might be possible without compromising any yield loss. Based on mean, range, genetic parameters, correlation coefficient and path coefficient values, direct selection through five traits i.e., head rice recovery percentage, cooking time, amylase content, protein content and thousand grain weight would significantly improve the grain yield of restorer line. Some of these restorer lines can be used for development of hybrid rice regarding these physicochemical, nutritional quality and yield related traits.
Keywords: Amylose, protein, milling, cooking quality, mean performance, heritability, correlation, path coefficient.
Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Postal Code 1706. Phone: +880-1716606098, E-mail address: umakanta@bsmrau.edu.bd
Published in „GENETIKA“ Vol. 52, No.3(2020), pp. 991-1007
© 2020 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003991B
Original scientific paper
Swarajyalakshmi N. BOLLINENI1, Bukya J. NAIK1, Suresh E. NAIK1, Gopalakrishnamurthy KADAMBARI1, G. Eswar REDDY1, S. MALATHI6, Eswar N.P. REDDY1, Srividhya AKKAREDDY2, Reddi M. SEKHAR3, Bahben TANTI4, B. Krishna VENI5, B. Vijaya LAKSHMI5 and Lakshminarayana R. VEMIREDDY1,3*
1Department of Molecular Biology and Biotechnology, S.V Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati-517502, Andhra Pradesh, India
2Regional Agricultural Research Station, ANGRAU, Tirupati-517502, Andhra Pradesh, India
3Department of Genetics and Plant Breeding, S.V Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati-517502, Andhra Pradesh, India
4 Department of Botany, Gauhati University, Guwahati, Assam, India
5Rice Research Unit, ANGRAU, Bapatla-522101, Andhra Pradesh, India
6Indian Institute of Rice Research, Hyderabad-500030, India
Abstract
Bollineni N.S., B.J. Naik, S. E. Naik, G. Kadambari, G. E. Reddy, S. Malathi, E.N.P. Reddy, S. Akkareddy, R. M. Sekhar, B. Tanti, B. K. Veni, B. V. Lakshmi, L. R. Vemireddy (2020). Genetic diversity, population structure and allele mining of genes governing grain size related traits in rice (Oryza sativa L.)- Genetika, Vol 52, No.3, 991-1007.
The present study was undertaken for allele mining of genes governing grain dimensions viz., grain length, grain width, grain thickness, grain size and grain weight. The molecular markers linked to the reported genes for these traits were used to screen 124 diverse rice genotypes. Thirty-two molecular markers used in this study produced a total of 86 alleles among 124 rice genotypes. The number of alleles ranged from 2 to 4 with an average of 2.58 alleles per locus. A dendrogram consisting of 124 rice genotypes revealed that all the genotypes can be divided into two groups. An analysis of the model-based population structure using simple sequence repeats (SSRs) covering all 12 chromosomes provided evidence of a significant population structure in the rice genotypes. The novel alleles identified in the study could be of great value for development of consumer-targeted rice varieties.
Keywords: Rice, Grain size, Allele mining, Population structure, and Marker-trait associations.
Corresponding author: Lakshminarayana R. Vemireddy, Department of Genetics and Plant Breeding, S.V Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU),
Tirupati-517502, Andhra Pradesh, India. Phone: +91 9440507965, E-mail: drvlnreddy@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1009-1019
© 2020Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003009B
Original scientific paper
GENETIC HOMOZYGOSITY AS A MARKER OF SMOKING DEPENDENCY
Slavko BRANKOVIC1, Dejan NIKOLIC2,3, Dragoslav MARINKOVIC4,
Suzana CVJETICANIN*5
1University of Pristina-Kosovska Mitrovica, Serbia
2Faculty of Medicine, University of Belgrade, Belgrade, Serbia
3Physical Medicine and Rehabilitation Department, University Children’s Hospital, Belgrade, Serbia
4Serbian Academy of Sciences and Arts, Belgrade, Serbia
5Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia
Abstract
Brankovic S., D. Nikolic, D. Marinkovic, S. Cvjeticanin (2020). Genetic homozygosity as a marker of smoking dependency. - Genetika, Vol 52, No3, 1009 - 1019.
The aim of our study was to evaluate the morphogenetic variability as a marker of smoking dependency in adult smokers versus controls and to investigate the presence and the degree of morphogenetic variability difference between male and female smokers versus same gender controls. The cross-sectional study evaluated 241 smokers and 185 nonsmoker individuals as controls. We analyzed 17 homozygous recessive characteristics (HRC). There was a significant difference in the individual variations of 17 HRCs between the controls and smokers (∑χ2=61.400, p<0.001; for females ∑χ2=79.440, p<0.001; for males ∑χ2=84.972, p<0.001). The mean values of HRCs significantly differed between smokers and controls (MV±SEM(Controls) –4.79±0.13, MV±SEM(Smokers) –5.70±0.12; p<0.001). For males, presence of 6/17 (35.29% genetic homozygosity) HRCs (OR=6.12) was to the certain degree predictor for smoking dependency. Higher degree of genetic homozygosity, changed variability and male gender, might be some among potential numerous factors that could have impact on smoking development and dependence.
Keywords: homozygous recessive characteristics; smoking; gender; predictors
Corresponding author: Suzana Cvjeticanin, Faculty of Medicine, University of Belgrade, Belgrade, Serbia, E-mail: cujasimsi@gmail.com, Phone: +381603534821
Published in „GENETIKA“ Vol. 52, No.3(2020), pp. 1021-1029
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
https://doi.org/10.2298/GENSR2003021R
Original scientific paper
MACHINE LEARNING APPROACHES TO CLASSIFY MELON LANDRACES BASED ON PHENOTYPIC TRAITS
Mohammad Reza NAROUI RAD1, Gholamali KEYKHA2, Jahangir ABBASKOOHPAYEGANI3
Ramin RAFEZI4
1Horticulture Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran
2 Soil and Water Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran
3Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 Horticultural Research Institute, Vegetable Research Department, Agricultural Research, Education and Extension Organization, AREEO, Karaj, Iran
Abstract
Rad Naroui R. M., G. Keykha, J. Abbaskoohpayegani, R. Rafezi (2020). Machine learning approaches to classify melon landraces based on phenotypic traits - Genetika, Vol 52, No.3, 1021-1029.
Phenotyping of native cultivars is becoming more essential, as they are an important for breeders as a genetic source for breeding. The variability of morphological properties plays critical role in melon breeding. In this paper various machine learning approaches were implemented to identify melon accession classes. A field experiment was conducted in Zahak Agriculture station to differentiate 144 melon accessions based on 14 traits. For this, Partial Least Square Discriminant Analysis (PLS-DA), Support Vector Machine (SVM), Random Forest (RF), K-Nearest Neighbor (KNN) and Classification And Regression Trees (CART) were compared. The most commonly used performance values comprise overall accuracy, kappa value, Receiver Operating Characteristics (ROC) and Area Under Curve (AUC) were performed to identify accuracy of the models. The results showed the best performance for CART than others. The AUC and kappa value were 0.85 and 0.80 and fruit weight was the most important trait that affecting diversity in melon accessions. Regarding to these results Classification And Regression Trees (CART) is reliable for identification of melon accessions classes.
Key words: Machine learning, Phenotype, Random forest, K-Nearest Neighbor
Corresponding author: Mohammad Reza Naroui Rad, Horticulture Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran, narouirad@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1031-1040
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003031K
Original scientific paper
THE POSSIBILITY OF BENOMYL AND DIAZINON PESTICIDE’S CARCINOGENICITY AND THE POTENTIAL OF HOTAIR AND H19 AS A SERUM BIOMARKER IN BREAST CANCER
Sara KARAMI1, Aras RAFIEE2*
1 Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2* Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract
Karami S. and A. Rafiee (2020). The possibility of benomyl and diazinon pesticide’s carcinogenicity and the potential of Hotair and H19 as a serum biomarker in breast cancer- Genetika, Vol 52, No.3, 1031-1040.
Evidence had grown ever stronger that pesticides can cause epigenetic modifications such as changing the expression of noncoding RNAs which have positive associations with incidence of cancer.
Upregulation of two oncogenic long non coding RNAs, HOX antisense intergenic RNA and H19, enhances breast cancer. This study was conducted to investigate and compare the effect of 7,12-dimethylbenz(a)anthracene (proven to cause breast cancer) with two commonly used pesticides named benomyl and diazinon (suspected of developing breast cancer) on the expression level of HOX antisense intergenic RNA and H19. Mice were intragastrically exposed to 7,12-dimethylbenzathracene, diazinon and benomyl for 60 days. The expression level of H19 and HOX antisense intergenic RNA were measured by Real-Time PCR. The findings revealed that the expression of long non coding RNAs in pesticides and 7,12-dimethylbenzathracene treated mice were significantly higher than untreated control. This study, for the first time, has demonstrated that diazinon and benomyl pesticides could cause upregulation of both oncogenic H19 and HOX antisense intergenic RNA. Since 7,12-dimethylbenz(a)anthracene induced breast tumors, similar results of all three experimental groups could be a testimony to the carcinogenicity of these pesticides and provides support for the importance of these noncoding RNAs as a target for therapeutic intervention in breast cancer.
Keywords: Pesticide, Biomarker, Breast cancer, H19, HOTAIR
Corresponding author: Aras Rafiee, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Oreg St. Hamilablovd, Poonk Sq. Tehran, Iran, E-mail: aras_marine_biology@yahoo.com. Tel: (+9821) 88074907, Fax: (+9821) 88385798
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1041-1053
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003041U
Original scientific paper
EFFECTS OF GRAFTING ON FRUIT YIELD AND LEAF NUTRIENT CONTENTS
OF PEPPER (Capsicum annuum L.) INBRED LINES
Firdes ULAS1*, Halit YETISIR1, Abdullah ULAS2
1Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri- Turkey.
2Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Erciyes University, Kayseri-Turkey.
Abstract
Ulas F., H. Yetisir, A. Ulas (2020). Effects of grafting on fruit yield and leaf nutrient contents of pepper (Capsicum annuum L.) inbred lines- Genetika, Vol 52, No.3, 1041-1053.
A field experiment was conducted to investigate the rootstock effects on the fruit and seed yield and leaf nutrient contents of pepper, four pepper inbred lines (ERÜ 1227 and ERÜ 462: Long fruited green pepper type; 21 H-1-2 and 33 H-3-1: Bell shaped pepper type) were grafted onto two pepper rootstock genotypes (46 KB F1 and Scarface F1). The non-grafted scion genotypes were used as control plants. Between grafted and non-grafted plants significant (p<0.001) differences were found in fruit yield, dry matter, diameter, length and fruit number, seed yield, thousand seed weight and leaf P, K, Ca, Mg, Mn and Zn contents. Among the graft combinations, significantly higher fruit yield, dry matter, length of long fruited green pepper genotypes, leaf K content was observed when ‘ERÜ 1227’ grafted onto ‘Scarface’ rootstock; significantly higher Ca and Mn contents were recorded when ‘ERÜ 462’ grafted onto ‘Scarface’ rootstocks. Whereas, significantly highest fruit number and diameter of long fruited green pepper genotypes was recorded at the non-grafted plants of ‘ERÜ 1227’. Overall, the pepper rootstock genotype ‘Scarface’ showed a better performance for fruit yield, dry matter, length of long fruited green pepper genotypes, fruit diameter of bell shaped pepper genotypes, K, Ca, Mg and Mn contents in leaf tissue, thousand seed weight in different graft combinations as compared to non-grafted pepper plants. All these suggest that the powerful rootstocks, which might be characterized by an efficient nutrient uptake, play a major role in improvement of plant growth performance, fruit, and seed yield.
Keywords: fruit diameter, rootstock, scion, seed yield, thousand seed weight.
Corresponding author: Firdes Ulas, Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri- Turkey, E-mail: fulas@erciyes.edu.tr, Tel:+90 537 919 3185
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp.1055-1073
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003055P
Original scientific paper
GENOTYPE BY YEAR INTERACTION EFFECTS ON SOYBEAN MORPHO-PRODUCTIVE TRAITS AND BIOGAS PRODUCTION
Vera POPOVIĆ1, Savo VUČKOVIĆ2, Zoran JOVOVIĆ3, Nikola RAKAŠČAN4, Marko KOSTIĆ5,
Nataša LJUBIČIĆ6, Milena MLADENOVIĆ-GLAMOČLIJA7, Jela IKANOVIĆ2
1 Institute of Field and Vegetable Crops, Novi Sad, Serbia
2University of Belgrade, Faculty of Agriculture, Zemun-Belgrade, Serbia
3University of Montenegro, Biotechnical Faculty, Podgorica, MNO
2University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
4University of Singidunum, Belgrade, Serbia
5Biosense Institute, Novi Sad, Serbia
6Institute of Applied Sciences, Belgrade, Serbia
Abstract
Popović V., S. Vučković, Z. Jovović, N. Rakaščan, M. Kostić, N. Ljubičić, M. Mladenović-Glamočlija, J. Ikanović (2020). Genotype by year interaction effects on soybean morpho-productive traits and biogas production-Genetika, Vol 52, No 3,1055-1073.
Biodiesel and biogas are especially important sources of renewable energy in the world and in Serbia. Biodiesel is used as transportation fuel; biogas is used for production of electricity and heat. Soybean (Glycine max L.) grain is the primary source of vegetable protein for food and feed supplements, also accounts for much of the world’s bio-oil supply. Due to the development of new technologies for processing agricultural waste into energy, the rate of increase in the use of alternative fuels is significantly increasing. The aim of this study was to determine the productivity of soybeans and the possibility of obtaining biogas from soybeans in divergent years, 2017-2019. Main effects were monitored, genotypes (G), years (Y) and genotype by years interaction effects (G x Y) and were used for evaluation of soybean genotypes in different environments. Soybean varieties were tested for mass of 1000 grain (MTG), plant height (PH), grain yields (GY) and biogas yield (BY). Stability of grain and biogas yield was determined to select best genotype. Results showed that genotype and years had a significant impact on all measured variables. In all tested years, the biggest GY and BY were at genotypes G1 (2.40–4.49 t ha-1, and 461.00–641.00 m3 ha-1, respectively) and G3 (1.97–4.30 t ha-1; i.e. 447.67–620.00 m3 ha-1). Genotype G2 had statistically significantly lower values for all tested parameters compared to other tested genotypes. Correlation analysis of some chosen traits showed different interdependence between measured variables depending on the year conditions. The results of this study pointed out that among best genotypes for production of grain and biogas were G1 and G3. From the results of this study it can be concluded that G x Y trials are important for evaluation of stability and choosing the most stable genotypes of soybean.
Keywords: soybean, genotype, grain yield, biogas yield, correlation
Corresponding author: Vera Popović, Institute of Field and Vegetable Crops, National Institute for the Republic of Serbia, Maksima Gorkog 30, 21000 Novi Sad, Serbia, E-mail: vera.popovic@ifvcns.ns.ac.rs;
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp.1075-1086
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003075N
Original scientific paper
MOLECULAR PHYLOGENY OF THE SECT. ADONIS (Genus Adonis L., Ranunculaceae)
Fatemeh NAJARIYAN1, Masoud SHEIDAI1*, Fahimeh KOOHDAR, Seyed Mehdi TALEBI2
1Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2Department of biology, Facility of sciences, Arak University, Arak, 38156-8-8349 Iran.
Abstract
Najariyan F., M. Sheidai, F. Koohdar, S. M. Talebi (2020). Molecular phylogeny of the sect. Adonis (genus Adonis L., ranunculaceae)- Genetika, Vol 52, No.3, 1075-1086.
The ornamentally important genus Adonis L. (Ranunculaceae) consists of about 30 species. In Iran, 8 species are recognized. The new classification system recognizes two subgenera, six sections, and six series for Adonis. The species differentiation within the genus is controversial due to morphological overlaps in different species and extensive morphological variation. Therefore, the aims of present study were: 1- Species delimitation within the Sect. Adonis based on morphological and molecular evidences and, 2- Studying the species relationship based on molecular phylogeny. Multivariate analyses of morphological and molecular (ITS and ISSR) data differentiated the Adonis species. PCA biplot analysis of morphological characters revealed morphological characters such as leaf arrangement; leaf length, flower diameter, petal length and width, calyx length, and pedicel length are of taxonomic implication in Adonis. Bayesian tree of ITS sequences revealed that the sect. Adonis is differentiated from the sect. Adonanthe. Based on ITS data, A. microcarpa showed close affinity to A. scrobiculata, while A. aestivalis, A. icrocarpa and A.dentata were close to each other.
Key words: Adonis; ITS; ISSR; Molecular phylogeny.
Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran, msheidai@yahoo.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1087-1106
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003087E
Original scientific paper
Mona Pishnamazzadeh EMAMI1, Nasser MOHEBALIPOUR2*, Ali Akbar EBADI3,
Hassan NOURAFCAN4, Jalil AJALI5
1Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.
2Department of Agronomy and Plant Breeding, Facutly of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.
3Assistant professor at Rice research Institute of Iran (RRII), agricultural research education and extension organization (AREEO), Rasht, Iran.
4Department of Horticulture, Faculty of Agriculture Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.
5Department of Agronomy and Plant Breeding, faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.
Abstract
Emami M. P., N. Mohebalipour, A. A. Ebadi, H. Nourafcan, J. Ajali (2020). QTL mapping of some morphological traits of rice in a RILS population derived from Hashemi and modified Nemat varieties hybrid - Genetika, Vol 52, No.3, 1087-1106.
In this study, QTLs controlling traits related to crop and grain yield were mapped using 140 recombinant inbred lines (F9 and F10) along with 4 control genotypes in anaugment experiment based on a randomized complete block design in 2consecutive years. Population linkage map was consisted of 170 SSR3 / 2132 centimorgan (cM) markers of rice genome, and mean distance between adjacent markers was equal to 12.47 centimorgan (cM). Composite Interval Mapping analysis was done for studying number of days to flowering in the first year, a QTL was identified on chromosome 3 and in the second year, three QTLs were identified on chromosomes 2, 3, and 7, which were common on chromosome 3 in both years. Regarding plant height in the first and second years, a QTL was mapped on chromosome 1, which was similar in both years. In terms of number of tillers in the first year, four QTLs were identified on chromosomes 4 and 12 and in the second year two QTLs were identified on chromosomes 11 and 12, and regarding panicle length in the first year, one QTL was identified on chromosome 6 and in the second year two QTLs were identified on chromosomes 2 and 4, and in terms of flag leaf length in the first year, two QTLs were identified on chromosomes 6 and 7 and in the second year two QTLs were identified on chromosome 7.With respect to flag leaf width in the first year, two QTLs were identified on chromosomes 1and 3, and in the second year, one QTL was identified on chromosome 11, and in relation to Panicle exertion in the first year, three QTLs were identified on chromosomes 1, 2, and 8, and in the second year, two QTLs were identified on chromosomes 1 and 2.In regard to yield in the first year, one QTL was identified on chromosome 2 and in the second year, two QTLs were identified on chromosome 2. Also, clusters of genes were identified by Interval Mapping in the population with respect to different traits on chromosomes 1 (two cases) and 12 (one case).
Keywords: Oryza sativa L., Quantitative trait locus, molecular markers, composite Interval Mapping.
Corresponding author: Nasser Mohebalipour, Department of Agronomy and Plant Breeding, Facutly of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran. E-mail: nasermohebalipor@yahoo.com. Tel:09113420569
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1107-1125
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2003107B
Original scientific paper
MAPPING WHEAT QTLS FOR GRAIN YIELD RELATED TRAITS UNDER HIGH TEMPERATURE STRESS
Mohamed N. BARAKAT*1, Abdullah A. Al-DOSS2, Khaled A. MOUSTAFA2, Mohamed I. MOTAWEI1,3, Ibrahim M. AL-ASHKAR2,4, Soleman M. AL-OTAYK3, Mohamed S. ALAMRI2, and Mohamed MERGOUM5
1Biotechnology Laboratory, Crop Science Department, Faculty of Agriculture, University of Alexandria, Egypt.
2College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
3Faculty of Agriculture and Veterinary Medicine, Qassim University, Buridah, Saudi Arabia.
4Agronomy Department, Faculty of Agriculture, Al- Azhar University, Cairo, Egypt.
5Department of Crop and Soil Sciences, College of Agriculture & Environmental Sciences, University of Georgia, USA.
Abstract
Barakat N. Mohamed, A.A. Al-Doss, K. A. Moustafa, M. I. Motawei, I. M. Al-Ashkar, S. M. Al-Otayk, M. S. Alamri, M. Mergoum (2020). Mapping wheat QTLS for grain yield related traits under high temperature stress- Genetika, Vol 52, No.3, 1107-1125.
Stress induced by high temperature represents a major constraint over wheat production in many production areas. Here, the comprehensive coverage of the wheat genome achievable using single nucleotide polymorphism markers was exploited to carry out a genetic analysis targeting yield components in plants exposed to high temperature stress. The mapping population was a set of doubled haploid lines derived from a cross between the cultivars Yecora Rojo and Ksu106. Both of the parental cultivars and their derived population were tested in the field in two locations over two consecutive seasons; at each site, two sowing dates were included, with the later sowing intended to ensure that the plants were exposed to high temperature stress during the grain filling period. Composite interval mapping detected 93 quantitative trait loci influencing grain yield and some related traits, along with 20 loci associated with a “heat susceptibility index” (HSI). The loci were distributed over all 21 of the wheat chromosomes. Some of these loci were of large enough effect to be considered as candidates for the marker-assisted breeding of high temperature tolerance in wheat.
Keywords: heat stress, grain yield, SNP markers, Triticum aestivum
Corresponding author: Mohamed N. Barakat, Biotechnology Laboratory, Crop Science Department, Faculty of Agriculture, University of Alexandria, Egypt, E-mail address: mnrbarakat@yahoo.com, Telephone: +201005225125
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1127-1144
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003127J
Original scientific paper
POPULATION DIFFERENTIATION AND GENE FLOW IN Erodium cicutarium:
A POTENTIAL MEDICINAL PLANT
Yaocheng JIA*1, Majid KHAYATNEZHAD2, Shahram MEHRI3
1University of Yuncheng, Department of arts and crafts design, Yuncheng, Shanxi, 044000
2Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
3Department of Agronomy and Plant Breeding, ParsAbad Moghan Branch, Islamic Azad
University, ParsAbad Moghan, Iran
Abstract
Jia Y., M. Khayyatnezhad, S. Mehri (2020). Population differentiation and gene flow in Erodium cicutarium: a potential medicinal plant- Genetika, Vol 52, No.3, 1127-1144
Erodium (Geranaiceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds. In arid and semi-arid regions, E. cicutarium has had some importance as a forage plant and is an important grazing plant and source of protein supplements to straw for ruminants in semi deserts and wastelands of the Middle East. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal importance of this species, a genetic variability and populations’ structure study is performed studying 15 geographical populations of E. cicutarium Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this plant. AMOVA test produced significant genetic difference (PhiPT = 0.39, P = 0.010) among the studied populations and also revealed that, 55% of total genetic variability was due to within population diversity while, 45% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. PCoA plot of populations based on morphological characters was in agreement with MDS plot of molecular data. These results indicated that geographical populations of E. cicutarium are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.
Keywords: E. cicutarium, Gene flow, Genetic differentiation, IRAP
Corresponding author: Yaocheng Jia, University of Yuncheng, Department of arts and crafts design, Yuncheng, Shanxi, 044000; E-mail: jinzhaoch@163.com , sh.mehri2000@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp.1145-1160
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2003145B
Original scientific paper
Antioxidant variability of wheat genotypes under salinity stress in situ
1University of Novi Sad, Faculty of Agriculture, Department of Field and Vegetable Crops, Novi Sad, Serbia
2 Institute of Field and Vegetable Crops, National Institute of the Republic of Serbia, Novi Sad, Serbia
Abstract
Banjac B., M. Dimitrijević, S. Petrović, V. Mladenov, D. Banjac, B. Kiprovski(2020). Antioxidant variability of wheat genotypes under salinity stress in situ. - Genetika, Vol 52, No3, 1145-1160.
Crop cultivation under the open-field conditions depends on a variety of biotic and abiotic factors which cause plant stress and deterioration. Due to high soil salinity, some soils can be an unfavourable growing environment for most plants. As a low productivity soil type, solonetz soils are a stressful growing environment, causing plant deterioration. Plants have developed a complex antioxidative defense system as a precaution against oxidative stress caused by high soil salinity. The trial was set up on a halomorphic soil type–solonetz. The research included ten cultivars, one local population of hexaploid wheat (Triticum aestivum ssp. aestivum L.) and one cultivar of triticale (Triticosecale W.). The activity of enzymatic and non-enzymatic antioxidants in plant antioxidative defense system was detected during the trial, as well as lipid peroxidation. The analysis of biochemical markers was done in the flowering stage, and then in the milk maturity phase. Research results of the tested components revealed the highest stress tolerance exhibited by genotypes Banatka and Bankut 1205. Understanding the process of oxygen radical production by the plant tissue contributes to breeding wheat cultivars for better stress tolerance. Selection of genotypes better adapted to growing conditions in solonetz soils could facilitate a more economically justifiable wheat production, and promote utilization of the lower-quality soil types in agriculture.
Keywords: wheat, solonetz, abiotic stress, antioxidative defense system
Corresponding author: Borislav Banjac, University of Novi Sad, Faculty of Agriculture Novi Sad, Sq. Dositeja Obradovića 8, 21 000 Novi Sad, phone: +381 (21) 485 32 99; E-mail:borislav.banjac@polj.edu.rs
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1161-1179
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003161P
Original scientific paper
DEVELOPMENT OF NEW MICROSATELLITE MARKERS FOR DNA FINGERPRINTING PATTERN OF BLACK GRAM (Vigna mungo L. Hepper)
AND GREEN GRAM (Vigna radiate L. Wilckzek)
Kaushik Kumar PANIGRAHI1*, Puranjaya PANIGRAHI2, Ayesha MOHANTY1, Purandar MANDAL1 and Basudeba SATAPATHY2
1Orissa University of Agriculture and Technology, Odisha, India.
2Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India
Abstract
Panigrahi K. K., P. Panigrahi, A. Mohanty, P. Mandal, B. Satapathy (2020). Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek) breeding lines - Genetika, Vol 52, No.3, 1161-1179.
SSR markers are considered to be the most ideal marker for genetic studies because they are multi-allelic, abundant, randomly and widely distributed throughout the genome, co-dominant that could differentiate plants with homozygous or heterozygous alleles, simple to assay, highly reliable, reproducible. Microsatellite markers are highly polymorphic and informative and could be successfully used for genome analysis in black gram & green gram. Microsatellite markers were used to evaluate genetic diversity in 17 indigenous cultivars of pulse crops (11 cultivars of green gram and 6 cultivars of black gram respectively). They are subjected to variability analysis with 26 microsatellite markers for identification efficient primers to conclude the nature of molecular diversity present among the pulses. The SSR primer G228 showed 63.63% of polymorphism followed by MB-SSR 238 (45.45%) and G006 (36.36%). The 12 microsatellite markers produced 15.90 % polymorphism with banding ranged up to 7 with an average of 2.3 polymorphic banding patterns per SSR primer. Similarly for black gram, three random microsatellite primers G006 (50%) and G166 and G204 (33.33%) revealed considerable DNA polymorphism. The 14 random SSR primers produced 8.33% of polymorphism with banding ranged up to three with an average of 1.28 polymorphic banding pattern per SSR primer. The Distinguish Power (D), Polymorphism Information Content (PIC) value and Marker Index (MI) values revealed some SSR primers like G006, G204 and G166 can alone amplified distinct banding pattern, where as a combination of (G228+G006), (G228+G304) for green gram and the combination (G006+G166) can be used for black gram for ascertaining genetic diversity at any stage of crop growth period for green gram or black gram. From the present study we can conclude that selective microsatellite markers are highly polymorphic, informative and easily reproducible, which can be successfully used either as single or with combination for molecular characterization of crop species belonging to Vigna species.
Keywords: microsatellite markers, molecular characterization, marker combinations, diversity
Corresponding author: Kaushik Kumar Panigrahi, Orissa University of Agriculture and Technology, Odisha, India, E-mail: kaushikpbg@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp 1181-1192
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003181P
Original scientific paper
ASSOCIATION STUDY OF MTHFR-C677T WITH MALE INFERTILITY AND REPORTING NEW POTENTIAL SNPS/ SEQUENCE VARIANTS AS A SOURSE OF POPULATION GENETIC MARKERS
Amir POURBORHANZADEH1, Masoud SHEIDAI1*, Zahra NOORMOHAMMADI2,
Naser KALHOR3, Mehdi Heidari HORESTANI1
1Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2Department of Biology, School of Basic Science, Science and Research Branch, Islamic
Azad University, Tehran, Iran
3Department of mesanchymal stem cell, Academic Center for Education, Culture and Research, Qom branch, Iran
Abstract
Pourborhanzadeh A., M. Sheidai, Z. Noormohammadi, N. Kalhor, M. H. Horestani (2020). Association study of MTHFR-c677t with male infertility and reporting new potential SNPS/ sequence variants as a sourse of population genetic markers- Genetika, Vol 52, No.3, 1181-1192.
It is estimated that about 15% of couples concern with the inability to conceive after 1 year of unprotected intercourse. Genetic and environmental factors play important roles in male infertility. MTHFR (Methylene Tetrahydro Folate Reductase) is encoded by a gene which maps to human chromosome 1p36.22. The c.677C>T mutation causes an amino - acid change of Alanine to Valine (Ala222Val) in exon 4 which is related to oligospermia and azoospermia. Our purpose was to investigate the association of rs1801133 in MTHFR gene with azoospermia and also genetic diversity of some Iranian folks. Peripheral blood samples of 100 men suffered from azoospermia and 55 controls collected from Infertility Clinic of Jihad-university of Qom. DNA was extracted by salting-out method and SNP-Genotyping was performed by PCR-RFLP technique and sequencing. Furthermore, statistical and population genetics analysis were done. The rs1801133 showed no significant relation with azoospermia according to statistical analysis. Furthermore, population genetics analysis showed that there is no genetic differentiation between Turk and Fars ethnics and case and control individuals. MTHFR studied SNP has no significant relation with azoospermia infertility, although further studies on more samples are demanded.
Keywords: Infertility, azoospermia, MTHFR gene, Single Nucleotide Polymorphism.
Corresponding author: Masoud Sheidai, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, E-mail : msheidai@yahoo.com, Tel: +989122593378
Published in „GENETIKA“ Vol. 52, No.3(2020), pp. 1193-1202
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.
https://doi.org/10.2298/GENSR2003193M
Original scientific paper
Biljana MILIĆ¹,², Biljana ERDELJAN³, Tanja JANKOVIĆ²,³, Milica POPOVIĆ¹,²,
Gordana STRAŽMEŠTER-MAJSTOROVIĆ¹,², Katarina ILIĆ⁴
¹Clinical Center of Vojvodina, Clinic for Nephrology and Clinical Immunology Novi Sad, Serbia
²University of Novi Sad, Faculty of Medicine, Novi Sad, Serbia
³Special hospital for rheumatic diseases Jodna Banja, Novi Sad, Serbia
⁴Roche,d.o.o., Belgrade, Serbia
Abstract
Milić B., B. Erdeljan, T. Janković, M. Popović, G. Stražmešter-Majstorović, K. Ilić (2020). Phenotype differences in hla-b27 positive versus negative patients with ankylosing spondylitis treated with tumor necrosis factor alpha inhibitors. - Genetika, Vol 52, No3, 1193-1202.
The human leukocyte antigen (HLA)-B27 is one of the strongest known genetic factors associated with the development of ankylosing spondylitis (AS), but approximately 10% of AS patients are HLA-B27 negative. The aim of this study was to compare clinical features and response to tumor necrosis factor-alpha inhibitor (TNF-α inhibitor) therapy in HLA-B27 positive and negative patients with AS. This retrospective analysis included AS patients treated with TNF-α inhibitor for at least 12 weeks in two referral centers for biologic therapy in Vojvodina province, Serbia. Clinical and demographic parameters were compared between HLA-B27 positive and negative patients. Data from 59 patients (59.32% male) were collected: 49 (83.05%) were HLA-B27 positive and 10 (16.95%) HLA-B27 negative. HLA-B27-positive patients showed higher family aggregation (49% vs. 10%; p=0.033) compared with those who were HLA-B27 negative. In contrast, HLA-B27 negative patients showed a higher prevalence of peripheral arthritis (49% vs. 90%; p =0.032) and longer diagnosis delay (8.42 vs. 5.73 years; p=0.016) but there were no differences regarding dactylitis, enthesitis, uveitis or inflammatory bowel disease (IBD). Also, no differences were observed between HLA-B27-positive and negative patients regarding disease activity prior to TNF-α-inhibitor therapy. All 59 patients who participated in the study has been administered at least one TNF-α inhibitor. The mean age at introduction of TNF-α inhibitor as well as mean disease duration from diagnosis until start of TNF-α inhibitor were similar between groups. HLA-B27 positive patients had a significantly longer drug survival time for first biologics (49.06±29.22 months), whereas HLA-B27 negative received it for 24.8±12.25 months (p<0.0000). 4/49 HLA-B27 positive (8.2%) and 1/10 HLA-B27 negative patients (10%) fail to demonstrate efficacy in AS (primary or secondary treatment failure) with no difference between groups. One HLA-B27 positive patient on etanercept developed IBD. All 6 non-responders switched to second TNF-α inhibitor and showed a good clinical response. In our cohort, presence of HLA-B27 was related to greater family occurrence, shorter diagnosis delay and lower peripheral arthritis rate. Moreover, HLA-B27 positive patients demonstrated significantly longer drug survival time for the first biologic then HLA-B27 negative, but non-response rate was similar between groups.
Keywords: HLA-B27, ankylosing spondylitis, tumor necrosis factor-alpha inhibitors, clinical features, response to therapy
Corresponding author: Biljana Milić, Clinical Center of Vojvodina, Clinic for Nephrology and Clinical Immunology, Hajduk Veljkova 1-3, Novi Sad, Serbia. E-mail:biljana.milic@mf.uns.ac.rs, +381637197561
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1203-1213
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2003203T
Original scientific paper
THE IMPACT OF AGROECOLOGICAL FACTORS ON MORPHOLOGICAL TRAITS OF MAIZE
Marijenka TABAKOVIĆ1*, Mile SEČANSKI1, Rade STANISAVLJEVIĆ2, Snežana MLADENOVIĆ DRINIĆ1, Milena SIMIĆ1, Jasmina KNEŽEVIĆ3, Violeta ORO2
1Maize Research Institute “Zemun Polje”, Belgrade, Serbia
2Institute for Plant Protection and Environment, Belgrade, Serbia
3Department for Crop Science, University of Prishtina, Faculty of Agriculture, Kosovska Mitrovica-Lešak, Serbia
Abstract
Tabaković M., M. Sečanski, R. Stanisavljević, S. Mladenović Drinić, M. Simić, J. Knežević, V. Oro (2020). The impact of agroecological factors on morphological traits of maize. - Genetika, Vol 52, No3, 1203-1213.
Climate changes are one of the crucial issues of modern agriculture. These changes imply the increase in average temperatures and the frequent occurrence of temperature extremes. Such conditions are stressful to maize concerning the expression of its traits.
This paper presents the analysis of the maize yield concerning the yield components, morphological and chemical traits under various agroecological conditions. The objective of the study was to evaluate variability of grain yield (GY) and yield components (ear length, EL and number of kernel rows, NKR), morphological traits (plant height,PH and ear height, EH), as well as chemical traits (the whole plant dry matter, PDM and the ear dry matter, EDM), and the effect of the environment on the intensity of expression of these traits. Fifteen genotypes developed by crossing of six inbred lines were used as a material in the study carried out during the two-year period in one location. Obtained results indicate that traits were more pronounced in maize hybrids than in the parental components including the lower variability of the traits expression. NKR did not affect the yield unlike the EL, whose coefficient of determination was R2 = 0.600 in both production years. The environmental effect was high for all traits except NKR. PDM and EDM were closely related to PH and EH, but they also depended on the EL and the NKR.
Variations in meteorological conditions have a significant impact on the final goal of the production, the yield. Prevailing agro-ecological conditions should be a major guideline in planning the crop production and selecting the genetic material.
Key words: yield, yield components, variability, environment
Corresponding author: Marijenka Tabaković, Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia. E-mail: marijenka.tabakovic@gmail.com
Published in „GENETIKA“ Vol. 52, No. 3(2020), pp. 1215-1233
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003215K
Original scientific paper
Rima KUMARI, Pankaj KUMAR, V.K. SHARMA*, Harsh KUMAR
Department of Agricultural Biotechnology and Molecular Biology
Dr. Rajendra Prasad Central Agricultural University, Pusa -848125 (Samastipur), Bihar, India
Abstract
Kumari R., P. Kumar, V.K. Sharma, H. Kumar (2020). Genome profiling of differential salt stress responsive landraces and varieties of rice using ISSR markers- Genetika, Vol 52, No.3, 1215-1233.
Using 14 ISSR primers for molecular profiling in relation to salinity tolerance of 18 landraces and varieties of rice, altogether 483 allelic variants including 236 shared and 247 unique alleles were generated with an average of 34.50 alleles per primer, revealing ample genetic differentiation and divergence amongst the entries under evaluation. Every primer generated polymorphic amplified products, but only 12 out of 14 primers yielded unique products. The primers having (AG)8YT, (CT)8A, (AG)8YA, (GA)8YT, (GA)8YC, (CT)8G, (TC)8C, (GATA)4 and (GA)8YG repeat motifs recorded relatively higher polymorphism per cent expressed in terms of the percentage of unique alleles in descending order of magnitude. Polymorphism information content of the primers varied from 0.612 to 0.992 for the primers (GACA)4 and (AG)8YA, respectively, with an average of 0.919 across the primers. Comparatively higher numerical values were obtained in respect of the primers (GA)8C, (CT)8A, (CT)8G, (TC)8C, (TC)8G, (AG)8YT, (AG)8YA, (GA)8YT, (GA)8YC, (GA)8YG and (GATA)4 amongst all the primers, reflecting their greater allelic richness and diversity. Poly-GA containing anchored primers produced the highest number (44.8) of allelic variants per primer followed by poly-CT, poly-AG and poly-TC containing anchored primers. But, the highest mean polymorphism per cent, highlighting the proportion of unique alleles, was exhibited by poly-AG followed by poly-GA, poly-CT and poly-TC containing anchored primers Clustering based on only poly-GA and poly-AG containing anchored primers provided more efficient genotypic discrimination in relation to salt stress responsiveness of the rice varieties. Moreover, a panel of only three poly-AG containing anchored primers facilitated perfect discrimination of rice varieties in accordance with their responsiveness to salinity stress. These primers can be efficiently utilized as functional instruments for connecting genotypic and phenotypic differences in relation to salt stress responsiveness. Principal coordinate analysis completely supported the results obtained from hierarchical classification of the landraces and varieties.
Keywords: Rice, salt tolerance, ISSR markers, genetic divergence
Corresponding author: V.K. Sharma, Department of Agricultural Biotechnology and Molecular Biology, Dr. Rajendra Prasad Central Agricultural University, Pusa -848125 (Samastipur), Bihar, India, E-mail: vksharmarau@yahoo.com; Phone – (+91)9546388475
Published in „GENETIKA“ Vol. 52 No. 3(2020), pp. 1235-1248
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003235P
Original scientific paper
Vladan POPOVIĆ1*, Aleksandar LUČIĆ1, Ljubinko RAKONJAC1,
Zoran MAKSIMOVIĆ2, Danijela RISTIĆ3
1Institute of Forestry, Belgrade
2SE Srbijašume, Belgrade
3Maize Research Institute, Zemun Polje, Belgrade
Abstract
Popović V., A. Lučić, Lj. Rakonjac, Z. Maksimović, D. Ristić (2020). Variability of morphological and anatomical characteristics of Serbian spruce (Picea omorika /Panč./ purkyne) needles of natural population located in the Milieševka river canyon. - Genetika, Vol 52, No3, 1235 - 1248.
Serbian spruce (Picea omorika /Panč./ Purkyne) is rare and endangered tertiary relict and endemic species in the Balkan Peninsula. Its natural distribution is fragmented and restricted to western Serbia and eastern Bosnia and Herzegovina, mainly in the canyon of middle course of the Drina river. Since the mid-19th century, the number and size of populations have been steadily decreasing accompanied by a decline in the number of mature individuals, endangering natural regeneration is endangered. The objective of this study is to determine variability within Serbian spruce (Picea omorika /Panč./ Purkyne) natural population in the canyon of the Mileševka river on the basis of morphological and anatomical characteristics of needles and, thus, to broaden knowledge on this rare and endangered species. Twigs with needles collected from all mature individuals that represent the population were used as material for the study. The following morphological and anatomical characteristics were measured: NL- needle length; NH - needle height; NW - needle width; CT+EH - cuticle + epidermis height; HH - hypodermis height; VB - vascular bundle diameter; No of SC - number of resin channels; SC diameter - resin channel diameter. The average values of the measured characteristics are: needle length 13.23 mm; needle width 1239.93 ɲm; needle height 608.63 ɲm; cuticle + epidermis height 11.49; hypodermis height 17.59 ɲm; vascular bundle diameter 220.64 ɲm; number of resin channels 0.74; resin channel diameter 52.36 ɲm. The characteristics with the highest variability are as follows: number of resin channels (161.64%); resin channel diameter (25.35%); hypodermis height (20.79%), while the needle width is a characteristic with the lowest variability (6.39%). The results of the analysis of variance showed very high statistical differences within population for all studied characteristics (p<0.01; α=0.05). In order to make the results clearer, it is necessary to investigate the genetic diversity and population structure by analyzing various phenotypic characteristics in specially designed experiments (e.g. progeny tests) as well as by analyzing appropriate DNA markers. The studied population must be involved in continuous conservation and breeding processes, especially through appropriate forms of ex situ conservation.
Keywords: Serbian spruce, variability, needle, length, hypodermis, population.
Corresponding author: Vladan Popović, Institute of Forestry, Kneza Višeslava 3, 11000 Belgrade, Serbia, Phone: 011-3553355, E-mail: vladanpop79@gmail.com
Published in „GENETIKA“ Vol. 52, No.3(2020), pp. 1249-1262
© 2020Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003249S
Oiginal scientific paper
DECREASE OF YIELD COMPONENTS AND MORPHOLOGICAL TRAITS OF SOYBEAN FULL-SIBS UNDER DROUGHT CONDITIONS
Mirjana SREBRIĆ, Zoran DUMANOVIĆ, Vesna PERIĆ, Violeta ANDJELKOVIĆ
Maize Research Institute “Zemun Polje”, Belgrade, Serbia
Abstract
Srebrić M., Z. Dumanović, V. Perić, V. Andjelković (2020). Decrease of yield components and morphological traits of soybean full-sibs under drought conditions - Genetika, Vol 52, No3, 1249-1262.
Soybean, as a plant species, is native to regions with conditions favourable of humidity and temperatures for its growth and development. The expansion of the soybean growing area resulted in requirements of varieties with higher tolerance and more stable grain yield under drought conditions. In order to develop high yielding varieties, the method of full-sib (FS) selection was applied in this study, as one of many methods of recurrent selection. The basic principle of recurrent selection is a cyclic selection, with the aim to accumulate favourable alleles. Three most yielding F2:3 sister lines obtained from the crossing combination of varieties Kunitz and Kador, were used for two combinations. Progenies of two FS crossing combinations were tested in two locations during two years (2011 with more favourable and 2012 with less favourable precipitation distribution). The values of grain yield per plant, the most important components of yield and morphological traits decreased under less favourable conditions. The highest decrease was recorded in the number of pods and the number of grains per plant, whereas the lowest decrease was found for the 1000-grain weight. Regardless of the relatedness of the tested material, significant differences in response to drought were observed not only between two FS progenies, but also within progenies of each combination. Soybean grain yield is a complex trait strongly affected by environmental conditions. Under unfavourable conditions, the reductions in the following traits were observed in progenies of two sister combinations: grain yield per plant (26.5-36.0% and 25.6-42.7%), number of pods per plant (18.6-33.0% and 12.6-38.2%) and the number of grains per plant (18.1-30.2% and 14.3-37.6%). Three progenies of FS crossings with the lowest yield reduction were identified as suitable initial material for the development of varieties with higher tolerance to drought conditions.
Keywords: soybean, full sib crossing, yield components, drought
Corresponding author: Mirjana Srebrić Maize Research Institute, Slobodna Bajića 1, Belgrade, E-mail:msrebric@mrizp.rs
Published in „GENETIKA“Vol. 52, No.3(2020), pp.1263-1279
© 2020 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2003263A
Original scientific paper
Slađan ADŽIĆ*, Zdenka GIREK, Suzana PAVLOVIĆ, Bogoljub ZEČEVIĆ,
Jelena DAMNJANOVIĆ, Dejan CVIKIĆ, Milan UGRINOVIĆ
Institute for Vegetable Crops, Smederevska Palanka, Serbia
Abstract
Adžić S., Z. Girek, S. Pavlović, B. Zečević, J. Damnjanović, D. Cvikić, M. Ugrinović (2020). Influence of different environmental conditions and gibberellic acid treatment on flowering time of divergent genotypes of cabbage (Brassica oleracea var. capitata L.) and their F1 hybrids. - Genetika, Vol 52, No3, 1263-1279.
In the process of cabbage breeding and seed production, one of the most important characteristics is the time of flowering. In order to investigate the influence of genotype, season and term of sowing on the flowering process, as well as the genetic control of this trait, an experiment was conducted with three genotypes of cabbage N, B and Scc of different geographical origin and different vegetation length, as well as three new F1 hybrids N x Scc , B x Scc and B x N obtained by hybridization between genotypes. The experiment was conducted during three temperature different seasons S1, S2 and S3 (average cold, cold and warm seasons), in three different sowing terms: August 15 (I), September 1 (II) and September 15 (III). During the winter period favorable for vernalization, two treatments with 300 ppm GA3 were performed. A statistically significant influence of all examined factors: genotype, year, sowing term and gibberellin GA3 treatment, on flowering time was determined. AMMI analysis determined the degree of adaptability of genotypes depending on the growing season, sowing terms and GA3 treatment for the trait time of flowering. The highest stability based on ASV values for flowering time was shown by hybrid BxN and its parental component genotypes B and N, while the hybrid Scc x B proved to be the most unstable in terms of flowering time. The pattern of relative expression of the most important flower repressor BoFLC2 gene showed a certain correlation with the flowering time of genotypes. The lowest quantitative expression of this gene was found in genotype B and it had the earliest flowering in all seasons, while genotype N had the highest relative expression of the BoFLC2 locus and the latest flowering.
Keywords: cabbage, time of flowering, gibberellic acid, expression BoFLC2 gene
Corresponding author: Slađan Adžić, Institute for Vegetable Crops, Karadjordjeva 71, 11420 Smederevska Palanka, Serbia, e-mail: sladjan.adzic@gmail.com, Phone: +381 26 317170, +381 64 8834 413
Published in „GENETIKA“Vol. 52, No.3(2020), pp. 1281-1290
© 2020 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2003281R
Original scientific paper
POLYMORPHIC STATUS AND PHYLOGENETIC ANALYSIS
OF MYOSTATIN GENE IN PAK-THOROUGHBRED
1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore - Pakistan
2 Faculty of Animal Husbandry, Institute of Animal and Dairy Sciences, University of Agriculture Faisalabad - Pakistan
3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore - Pakistan
4Department of Livestock Production, University of Veterinary and Animal Sciences, Lahore-Pakistan
Abstract
Raza Z. H., A. Nadeem, M. Javed, F.-ul-Hassan, W. Shehzad, A. Ahmad, M. Saadullah, M.M.Ali, (2020). Polymorphic status and phylogenetic analysis of myostatin gene in Pak-thoroughbred.- Genetika, Vol 52, No3, 1281-1290.
Myostatin is a protein translated by the the MSTN gene (also known as GDF8), is responsible for limiting muscle growth and strength. In thoroughbred horse (Equus Caballus), limited studies have been designed to examine the variants in the coding region of MSTN gene. However, no data is available regarding the single nucleotide polymorphisms (SNPs) of the MSTN about racing performance in thoroughbred horses of Pakistan. In this study blood samples of fifteen Pakistani thoroughbred horses were collected from Race Club Lahore and immediately transferred into the ice box. The DNA was extracted by using phenol-chloroform method. Primers were designed for the amplification of all exons of the MSTN gene. The amplified PCR products were precipitated and sequenced for the identification of SNPs. SNPs were identified by visualizing the peaks of sequenced data by using Chromas Software. Phylogenetic analysis of MSTN gene in Pak-thoroughbred with racing species and some breeds of horses like Marwari Indian breed, Sindhi breed, Kathlawari breed, Italian breed and Chines breed was done separately by using MEGA 6 software. The analysis of identified SNPs were carried out by software SNPator. The sequenced data with altered protein was published in GenBank with accession number MN604194. Results have shown a total of 3 single nucleotide polymorphisms through Blast with reference sequences. Two SNPs were found in exon 2 at position of 2406 (C/T) and 2408 (C/T) respectively. One SNP (T/C) was detected in exon 3 at the position of 4661. In conclusion, Pak-thoroughbred horse population has 3 polymorphisms in their coding region which can be used as a biological marker for athletic abilities in Pak- thoroughbred.
Keywords: myostatin, Pak-thoroughbred, polymorphism, horses, phylogenetic analysis
Corresponding author: Asif Nadeem, Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Email: asifnadeem@uvas.edu.pk, Phone # +92-347-4446413