GENETIKA, Vol

GENETIKA, Vol. 53, No.2 (2021)

Blaženka PETRIČEVIĆ and Dragica PEŠUT

ABO BLOOD TYPE ANALYSIS IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE [Abstract] [Full text]

Jaskanwal SINGH, R.K. DHALL and Yogesh VIKAL

GENETIC DIVERSITY STUDIES IN INDIAN GERMPLASM OF PEA (Pisum sativum L.) USING MORPHOLOGICAL AND MICROSATELLITE MARKERS [Abstract] [Full text]

Lucas SAMPAIO, Rafaela Priscila ANTONIO, Marilza Neves do NASCIMENTO,Paulo Ivan FERNANDES-JÚNIOR

MOLECULAR APPROACHES FOR IDENTIFICATION OF THE APOMICTIC/SEXUAL REPRODUCTIVE MECHANISM AND GENETIC VARIABILITY IN BUFFEL GRASS (Cenchrus spp.) ACCESSIONS [Abstract] [Full text]

Lejing LIN, Li LIN, Abdul WAHEED

ASSESSMENT OF GENETIC STRUCTURE AND DIVERSITY OF Erodium (Geranaiceae) SPECIES [Abstract] [Full text]

Evgeniy DIMITROV, Zlatina UHR, Nikolaya VELCHEVA

GENETIC DISTANCE OF COMMON WINTER WHEAT VARIETIES [Abstract] [Full text]

Dobrila JANKOVIĆ, Božidarka MARKOVIĆ, Radica DJEDOVIĆ, Snežana TRIVUNOVIĆ, Momčilo ŠARAN

GENETIC PARAMETERS OF THE TYPE TRAITS OF HOLSTEIN-FRIESIAN PRIMIPAROUS DAIRY COWS [Abstract] [Full text]

Zhana IVANOVA and Nikolay VELKOV

GENETIC VARIABILITY IN BULGARIAN MELON COLLECTION - FLOWERING TYPES AND FRUIT QUANTITATIVE TRAITS [Abstract] [Full text]

Zhao YANPENG, Wang HONGMEI, Liang WEI, Majid KHAYATNEZHAD and FAISAL

GENETIC DIVERSITY AND RELATIONSHIPS AMONG SALVIA SPECIES BY ISSR MARKERS [Abstract] [Full text]

Dilek KAYA and Zeynel DALKILIÇ

DETERMINATION OF A SEX-RELATED RAPD MARKER IN CAROB (Ceratonia siliqua L.) [Abstract] [Full text]

Hüseyin UYSAL and Emre SEVİNDİK

DEVELOPMENT OF BREEDING LINES BY ZIGOTIC OVULE CULTURE IN Nigella sativa L. [Abstract] [Full text]

Firdes ULAS

RESPONSE OF Different Rootstocks on Vegetative Growth, fruıt and Seed Yield of Eggplant (Solanum melongena L.) [Abstract] [Full text]

Sakine NAJAFIAN, Iraj MEHREGAN, Alireza IRANBAKHSH^,Mostafa ASSADI, Silvio FICI

SPECIES DELIMITATION IN CAPPARIS (CAPPARACEAE): MORPHOLOGICAL AND MOLECULAR [Abstract] [Full text]

Sukhninder KAUR, Mohinder Kaur SIDHU, Ajmer Singh DHATT

GENETIC DIVERSITY ANALYSIS THROUGH CLUSTER CONSTELLATION IN BRINJAL (Solanum melongena L.) [Abstract] [Full text]

Iva SAVIĆ, Milica NIKOLIĆ, Ana NIKOLIĆ, Ivana VICO, Snežana MLADENOVIĆ DRINIĆ, Nataša DUDUK, Slavica STANKOVIĆ

THE Morfological and Molecular identification of Fusarium verticillioides causing fusariosIs on wheat grain [Abstract] [Full text]

Xin MA^,Huailiang TIAN² Haiou XIA, ZEENAT

GENETIC DIVERSITY OF Lonicera L. (Caprifoliaceae) ESTIMATED BY MOLECULAR MARKERS AND MORPHOLOGICAL CHARACTERS [Abstract] [Full text]

Bojana PETROVIĆ and Milica KOMNENIĆ RADOVANOVIĆ

CYTOGENETIC ASPECTS OF MISCARRIAGE [Abstract] [Full text]

Mojdeh MAHDAVI, Fariba SHARIFNIA, Fahimeh SALIMPOUR, Akbar ESMAEILI, Mohaddeseh LARYPOOR

GENETIC DIVERSITY AND POPULATION STRUCTURE OF IRANIAN PISTACHIO (Pistacia vera L.) CULTIVARS [Abstract] [Full text]

Milica BLAŽIĆ, Dejan DODIG, Vesna KANDIĆ, Dragoslav ĐOKIĆ, Tomislav ŽIVANOVIĆ

GENOTYPIC VARIABILITY OF ROOT AND SHOOT TRAITS OF BREAD WHEAT (Triticum aestivum L.) AT SEEDLING STAGE [Abstract] [Full text]

Chao WAN, Fang ZHANG, Liangming ZHU

EXPRESSION OF CAVEOLIN-2 IN PATIENTS WITH ORAL CANCER AND CORRELATIONS WITH CLINICOPATHOLOGICAL PARAMETERS [Abstract] [Full text]

Hulya DOGAN

COMPOSITIONAL DIVERSITY IN ESSENTIAL OIL OF Ziziphora tenuior L. ECOTYPES [Abstract] [Full text]

Mohammad VAHEDIand Masoud SHEIDAI

ASSOCIATION STUDY OF RS535296987 IN TEX14 GENE IN AZOOSPERMIAMEN: RFLP AND DNA SEQUENCING [Abstract] [Full text]

Haiying LI, Yingxue WANG, Rabia IQBAL

SCOT MOLECULAR MARKERS AND POPULATION DIFFERENTIATION IN Hedera helix L. [Abstract] [Full text] [Supplement]

Faegheh BEHZADIFAR, Sayeh Jafari MARANDI, Ahmad MAJD, Fahimeh SALIMPOUR, Sedigheh ARBABIAN

GENETIC DIVERSITY ANALYSIS OF THE MEDICINAL PLANT Heracleum persicum Desf. ex Fischer [Abstract] [Full text]

Kuanhong MENG, Jia YAO, CongYing HE, Hakimeh MORABBI HERAVI

GENE FLOW AND GENETIC STRUCTURE BETWEEN POPULATIONS OF Hesperis L. (BRASSICACEAE) SPECIES USING MOLECULAR MARKERS [Abstract] [Full text]

Xiaohui QIAN and Shahram MEHRI

DETECTING DNA POLYMORPHISM AND GENETIC DIVERSITY IN A WIDE PISTACHIO GERMPLASM BY RAPD MARKERS [Abstract] [Full text] [Supplement]

Weimiao CHEN, Majid KHAYATNEZHAD, Nima SARHADI
GENE FLOW AND POPULATION STRUCTURE IN ALLOCHRUSA (CARYOPHYLLOIDEAE, CARYOPHYLLACEAE) WITH THE USE OF MOLECULAR MARKERS [Abstract] [Full text]

Sanja RAŠČANIN, Mirjana JOVANOVIĆ², Dejan STEVANOVIĆ, Nemanja RANČIĆ⁵

QUESTIONNAIRE FOR EVALUATING INFORMATION, KNOWLEDGE, AND ATTITUDES ON DONATION, STORAGE, AND APPLICATION OF INDUCED PLURIPOTENT STEM CELLS [Abstract] [Full text] [Supplement]

Mithlesh KUMAR, Manubhai PATEL, Ravindrasingh CHAUHAN, Chandresh TANK, Satyanarayan SOLANKI

DELINEATING MULTIVARIATE DIVERGENCE, HERITABILITY, TRAIT ASSOCIATION AND IDENTIFICATION OF SUPERIOR OMEGA-3-FATTY ACID SPECIFIC GENOTYPES IN LINSEED (Linum usitatissimum L.) [Abstract] [Full text]

Fahimeh FALLAH and Farrokh GHAHREMANINEJAD

PALYNO-MORPHOLOGY OF Hedera L. (the ivy genus, Araliaceae) AND THEIR SYSTEMATICS IMPLICATIONS [Abstract] [Full text] [Supplement]

Liu hang, Li pan, Tang yong, Luo jianguo, Xu XINGMIN, FAISAL

POPULATION GENETIC STRUCTURE AND GENE FLOW IN Alcea aucheri (BOISS.) ALEF.: A POTENTIAL MEDICINAL PLANT[Abstract] [Full text] [Supplement]

Xinxin ZHANG and Abdul SHAKOOR

STRONG GENETIC DIFFERENTIATION OF THE Paracaryum SPECIES (Boraginaceae) DETECTED BY INTER-SIMPLE SEQUENCE REPEATS (ISSR) [Abstract] [Full text]

Huixing LI, Haiyan YU, Xiancai ZENG, Sahar HUSSEIN HAMARASHID

STUDY ON GENETIC DIVERSITY BETWEEN Malva L. (Malvaceae): A HIGH VALUE MEDICINAL PLANT USING SCOT MOLECULAR MARKERS [Abstract] [Full text]

Vladimir MILADINOVIĆ, Stefan KOLAŠINAC, Ilinka PEĆINAR, Biljana KIPROVSKI, Dragosav MUTAVDŽIĆ, Vladan UGRENOVIC, Zora DAJIĆ STEVANOVIĆ

EFFECTS OF GENOTYPE AND BRADYRHIZOBIUM INOCULATION ON MORPHOLOGICAL TRAITS, GRAIN YIELD AND PROTEIN CONTENT OF SOYBEAN VARIETIES [Abstract] [Full text]

Shu-Chen MOWANG, Fu-Ji CHEN, Zeenat

STUDY ON GENETIC DIVERSITY BETWEEN Erodium (Geranaiceae) SPECIES BASED ON INTER-SIMPLE SEQUENCE REPEAT MARKERS [Abstract] [Full text]

Editor

CORRIGENDUM Yaocheng JIA, Majid KHAYYATNEZHAD, Shahram MEHRI [Abstract] [Full text]

Editor

CORRIGENDUM Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN [Abstract] [Full text]

Published in „GENETIKA“ Vol. 53, No.2 (2021), pp. 457-471

UDC 575.
https://doi.org/10.2298/GENSR2102457P
Orginal scientific article

ABO BLOOD TYPE ANALYSIS IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Blaženka PETRIČEVIĆ¹* and Dragica PEŠUT^2,3

¹* Bureau for Education Services, Podgorica, Montenegro

²Department of Internal Medicine, Medical Faculty of the University of Belgrade

³Teaching Hospital of Pulmonology, University Clinical Center of Serbia, Belgrade, Serbia

Abstract

Petričević B. and D. Pešut (2021). ABO blood type analysis in patients with chronic obstructive pulmonary disease. - Genetika, Vol 53, No.2, 457- 471.

Tobacco smoking is major risk factor for development of chronic obstructive pulmonary disease (COPD), which appears in 15-20% of smokers. Apart from smoking, exposure to polluted air and various noxae, and several genetic factors influence its development as well. The ABO blood type distribution varies among populations in the world, but also within subpopulations. A large number of studies have shown a correlation between blood types and the pathology of various diseases. These markers, used in population genetic research, have mainly shown deviations in the representation of blood groups in different diseases, compared to the general population. The aim of this study was to determine the ABO blood types distribution in patients with COPD compared to the general population, and their possible association with COPD stage, patients’ nutritional status and lung function impairment. This observational, prospective study included 150 patients (68.7% men and 31.3% women), average age 64.80 ± 8.38 years, diagnosed with COPD. Data were collected at the Clinical Center of Montenegro in Podgorica and at the Special Hospital for Lung Diseases Brezovik in Niksic. Determination of blood types of the ABO system and Rh factors for all subjects was performed at the Blood Transfusion Center in Podgorica. Apart from patients’ tobacco smoking status (duration of smoking and the number of cigarettes smoked per day expressed in pack/years for current smokers and former smokers), we also analyzed their exposure to various other noxae, their body mass index, and lung function in correlation to ABO blood type and Rh factor, and performed statistical analysis. We found a significant difference in the distribution of ABO blood types in patients with COPD compared to the general population. The highest frequency of blood type A was found in patients with COPD. We also found the lowest average values of spirometry parameters in that group, which represented majority of those patients with respiratory insufficiency having the most severe stage of the disease. Combined blood types A, B and AB were significantly more common in patients with COPD in comparison to blood type O, which is the least represented (23.3%). The least obstructive disturbance of pulmonary ventilation was found in the patients with B type. Respiratory insufficiency showed differences in gender representation, found in 40.4% of women, and in 25.2% of men with terminal phase of COPD. The prevalence of AB, higher than expected, decreases with the severity of the disease.

Key words: chronic obstructive pulmonary disease – COPD, ABO blood type, Rh factor, lung function,respiratory insufficiency

Corresponding author: Blaženka Petričević, Vaka Djurovica bb, 81000 Podgorica, Montenegro, e-mail: bazenkap9@gmail.com, Telephone +382 69 594 515

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Published in „GENETIKA“ Vol. 53, No. 2 (2021), pp.473-491

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2102473S
Original scientific paper

GENETIC DIVERSITY STUDIES IN INDIAN GERMPLASM OF PEA

(Pisum sativum L.) USING MORPHOLOGICAL AND MICROSATELLITE MARKERS

Jaskanwal SINGH¹, R.K. DHALL^*1 and Yogesh VIKAL²

¹Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141004, India

²School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141004, India

Abstract

Singh J., R.K. Dhall and Y. Vikal (2021). Genetic diversity studies in Indian germplasm of pea (Pisum sativum L.) using morphological and microsatellite markers. - Genetika, Vol 53, No.2, 473- 491.

Forty seven garden pea genotypes were characterized using thirty four pea specific microsatellite markers. Mean square values were highly significant for all the characters. Nearly 75% of the total variation was contributed by 3 traits viz. green pod yield per plant (43.18), plant height (20.91) and number of nodes per plant (10.27). 28 polymorphic SSR markers were used. Average PIC value was 0.55. NTSYS clustering based on UPGMA grouped the genotypes into two major groups I and II (dwarf and tall, respectively). The larger group consisted of 43 genotypes (subgroups I-VII) and smaller group had 3 genotypes (subgroup VIII). One genotype (PMR-19) separated from other genotypes. The dendrogram depicted overall similarity coefficient ranged from 0.59 to 0.89. The lowest genetic similarity (0.39) was observed between major group I and major group II. Maximum genetic similarity (0.89) was found between two pairs i.e. PB-89 and C-96 (first pair) and Angoori and GS-10 (second pair). Neighbor joining tree thus obtained highly corresponds to the clustering observed using NTSYS indicating that the analysis was reliable. The findings of the present study can be used to select diverse genotypes to be used as parents of crosses aimed for breeding improved pea cultivars.

Key words: pea, genetic variability, morphological traits, SSR markers.

Corresponding author: R R.K.Dhall, Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, Punjab, INDIA. Ph (M): +91-8283840078, E-mail: rajinderkumar@pau.edu

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Published in „GENETIKA“ Vol. 53, No. 2 (2021), pp.493-505

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102493S

Original scientific article

MOLECULAR APPROACHES FOR IDENTIFICATION OF THE APOMICTIC/SEXUAL REPRODUCTIVE MECHANISM AND GENETIC VARIABILITY IN BUFFEL GRASS (Cenchrus spp.) ACCESSIONS

Lucas SAMPAIO¹, Rafaela Priscila ANTONIO², Marilza Neves do NASCIMENTO¹,

Paulo Ivan FERNANDES-JÚNIOR^2*

¹Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, Brazil

²Embrapa Semiárido, Petrolina, Pernambuco, Brazil

Abstract

Sampaio L., R. P. Antonio, M. N. do Nascimento, P. I. Fernandes-Júnior (2021). Molecular approaches for identification of the apomictic/sexual reproductive mechanism and genetic variability in buffel grass (Cenchrus spp.) accessions. - Genetika, Vol 53, No.2, 493- 505.

Cenchrus spp. encompasses forage grasses that are especially important for drylands. Thus, information on their reproductive mechanism and genetic diversity is needed. The Active Germplasm Bank (AGB) of the Embrapa Semiárido (Tropical semiarid research center) has 115 accessions of Cenchrus spp. that were not molecularly characterized. Therefore, the objective of this work was to evaluate the genetic diversity of Cenchrus spp. accessions in the AGB, and identify their reproductive mechanisms using DNA markers. Specific SCAR markers Q8H, UGT197, and PCAB10, in addition to the SCAR marker 4HS* were used to identify genotypes with apomictic and sexual reproduction modes, respectively; the genetic diversity was characterized using the ISSR D12 (GA) 6CG and HB14 (CTC)3GC markers. The reproductive mechanism was analyzed using images for the presence or absence of the band in the expected amplicon size. For the analysis of genetic diversity, the gel images were exported to the BioNumerics Software (Applied Maths), in which polymorphism data were obtained and the dissimilarity dendrogram constructed by the UPGMA clustering method using the Dice coefficient. The markers related to the apomictic region were positive for all accessions. However, only the accessions CPATSA 102 and CPATSA 134 had positive markers using the primers for both reproduction strategies, indicating that these individuals can be facultative sexual. The cluster analysis discriminated two large groups and the accessions clustered mainly by the place of origin. It was also possible to identify duplicates within AGB. The identification and exclusion of duplicate accessions provides financial and labor savings in maintaining and managing the AGB. The two sexual accessions were selected for the breeding programs of Cenchrus spp. in Brazil.

Key words: apomixis, selection assisted by markers, forage grasses, germplasm, breeding

Corresponding author: Paulo Ivan Fernandes-Júnior, Embrapa Semiárido, BR 428, km 152, s/n, Petrolina, Pernambuco, Brazil, Phone: ++ 55 87 3866 3828, Fax: ++ 55 87 3866 3615, E-mail: paulo.ivan@embrapa.br

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Published in „GENETIKA“ Vol. 53, No2(2021), pp. 507-520

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102507L
Original scientific article

ASSESSMENT OF GENETIC STRUCTURE AND DIVERSITY OF Erodium (Geranaiceae) SPECIES

Lejing LIN^1*, Li LIN¹ and Abdul WAHEED²

¹College of Landscape and Ecology，Ningbo City College of Vocational Technology, Ningbo Zhejiang, 315100, China

²Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan

Abstract

Lin L., L. Lin and Waheed A. (2021). Assessment of genetic structure and diversity of Erodium (Geranaiceae) species. - Genetika, Vol 53, No.2, 507-520.

Erodium Aiton (Geraniaceae) with 75 species is distributed in all continents except Antarctic. Its main diversification center is Mediterranean region with 62 species. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Erodium species, were studied through a combination of morphological and molecular data. 70 individuals related to seven Erodium were collected in 7 provinces. A total of 96 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Erodium species. These bands were produced with the combinations of six selective primers. The total number of amplified fragments ranged from 10 to 25. The genetic similarities between seven species are estimated from 0.70 to 0.85. Clustering results showed two major clusters. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Erodium species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.

Key words: Sequence-related amplified polymorphism, Gene Flow; Genetic Diversity, Morphometric Analysis, Erodium species

Corresponding author: Lejing Lin, College of Landscape and Ecology, Ningbo City College of Vocational Technology，Ningbo Zhejiang, 315100, China,Email: linlejing01 @163.com

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Published in „GENETIKA“ Vol. 53, No.2 (2021), pp. 521-532

Serbia

UDC 575.633.11
https://doi.org/10.2298/GENSR2102521D
Original scientific paper

GENETIC DISTANCE OF COMMON WINTER WHEAT VARIETIES

Evgeniy DIMITROV, Zlatina UHR, Nikolaya VELCHEVA

Agricultural Academy

Institute of Plant Genetic Resources „K. Malkov“, Sadovo, Bulgaria

Abstract

Dimitrov E., Z. Uhr, N. Velcheva (2021). Genetic distance of common winter wheat varieties. - Genetika, Vol 53, No.2, 521-532.

The study was conducted during the period 2017-2019 in the experimental field of IPGR Sadovo. Wheat is the most important agricultural crop worldwide. Bulgarian wheat production in 2019 amounts to 6.162 thousand tons - an increase of 5.7% compared to 2018, due to the relatively favorable climatic conditions in the process of crop development, which led to an increase in average yield (with 6.8%). Twenty nine common winter wheat varieties (Triticum aestivum L.) with Belorusian origin and two Bulgarian varieties – Sadovo 1 and Enola were evaluated. The studied materials were sown in 2 rows with the length – 2 m, at the row spacing – 20 cm and the distance in rows – 5 cm. The standard technology adopted in IPGR for cultivation of common winter wheat was used. The aim of this study is to investigate the genetic distance of common winter wheat genotypes with a view to their use in the breeding process as sources of initial material for the formation of new and highly productive wheat varieties. Biometric measurements were made on 20 plants of each genotype and the main productivity indicators were recorded. To evaluate the genetic similarity between the different varieties, cluster analysis and PC analysis were applied. Based on the cluster analysis, the studied genotypes are classified into five large cluster groups. With the most genetic closeness to each other are characterized the varieties Vasilisa and Uzlet, followed by Daria and Rostan. The greatest genetic distance is shown between the Bulgarian varieties Sadovo 1 and Enola to the varieties from Belarus. The greatest genetic difference was found between Sadovo 1 and Rassvet, followed by Enola and Rassvet. Genetically distant varieties belonging to different cluster groups can be used as sources of initial material to achieve genetic diversity in the breeding of common winter wheat.

Key words: breeding, genetic diversity, genetic distance, Triticum aestivum L.

Corresponding author: Zlatina Uhr, Institute of Plant Genetic Resources „K. Malkov“, Sadovo, Bulgaria, E-mail: zlatinapg@abv.bg, phone: +359 88 5 036 368

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 533-544

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102533J

Original scientific paper

GENETIC PARAMETERS OF THE TYPE TRAITS OF HOLSTEIN-FRIESIAN PRIMIPAROUS DAIRY COWS

Dobrila JANKOVIĆ^1*, Božidarka MARKOVIĆ², Radica DJEDOVIĆ³,

Snežana TRIVUNOVIĆ¹, Momčilo ŠARAN¹

¹Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia

²Department of Livestock Science, Biotechnical Faculty, University of Montenegro, Podgorica, Montenegro

³Department of Animal Breeding, Faculty of Agriculture, University of Belgrade, Zemun, Serbia

Abstract

Janković D., B. Marković, R. Djedović, S. Trivunović, M. Šaran (2021). Genetic parameters of the type traits of Holstein-Friesian primiparous dairy cows. - Genetika, Vol 53, No.2, 533-544.

The aim of this study was to determine the level and variability of type traits in primiparous Holstein-Friesian cows, as well as to assess the additive genetic variance of type traits and heritability coefficients. The research was conducted on a data set of linearly evaluated primiparous Holstein-Friesian cows, which is ceded by the Main dairy cattle breed organization in the Province of Vojvodina, which is a unit of the Department of Animal Science, at the Faculty of Agriculture in Novi Sad. Data of 24226 primiparous Holstein-Friesian cows, refer to the period 2012-2015, were analysed. The phenotypic variability of the type traits, as well as effects of systemic factors affecting these traits, were analyzed using standard statistical methods in the Statistica software 13.2, and the general linear model (GLM). A Statistically highly significant effect for most of the type traits had the farm size, interaction of the year and the season of evaluation, age at linear evaluation, lactation stage and a genetic group formed by the year and country of bull/sires birth. The total type traits score (final score), which represents an evaluation of the overall primiparous cow exterior, is calculated for the 24226 linearly evaluated primiparous cows. Estimation of variance components and heritability coefficient calculation is done in the software package WOMBAT. Heritability coefficient values for the primiparous cow's type traits in the Province of Vojvodina were low to middle, ranged from 0.08 for the rear legs - side view up to 0.38 for the stature. Due to the fact that the value of the heritability coefficient depends on the variability of the traits and previous selection, lower heritability may be a consequence of the low genetic variability, or, a higher degree of variability which is a consequence of the effect of the systematic factors. Heritability coefficients indicate a genetic variability of the type traits, and its evaluation is important for the breeding value estimation for the type traits.

Key words: Holstein Friesian primiparous cows, type traits, variance components, additive genetic variance, heritability.

Corresponding author: Dobrila Janković, ¹Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia, Phone: +381 21 485 3429 E-mail: dobrila.jankovic@stocarstvo.edu.rs

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Published in „GENETIKA“ Vol. 53, No2(2021), pp545-558

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2102545I
Original scientific paper

GENETIC VARIABILITY IN BULGARIAN MELON COLLECTION - FLOWERING TYPES

AND FRUIT QUANTITATIVE TRAITS

Zhana IVANOVA and Nikolay VELKOV

Maritsa Vegetable Crops Research Institute, Department of Plant Breeding, Plovdiv, Bulgaria

Abstract

Ivanova Z. and N. Velkov (2021). Genetic variability in Bulgarian melon collection - flowering types and fruit quantitative traits. - Genetika, Vol 53, No.2, 545-558.

Cucumis melo L. is characterized with a great polymorphism according to many agronomical features. Study the variability of melon flowering and fruit traits is an initial step in the breeding program. The current study aimed to identify the genetic variability in the melon collection according to flowering and fruit traits in order to be used in breeding programs. It was evaluated fifty melon genotypes belong to different groups - cantalupensis, reticulatus, makuwa, inodorus, and agrestis. The studied collection consists of Bulgarian old cultivars, hybrids, landraces, PI, and introduced lines. Investigated genotypes were characterized by nine quantitative fruit traits - days to flowering, sex expression, days to ripening, fruit length, fruit diameter, flesh thickness, fruit weight, seed cavity diameter, and total soluble solids. It was established great variability in studied characteristics measured by a coefficient of variation from 7.73 to 39.85%. Significant correlations between fruit length, fruit diameter, flesh thickness, cavity diameter, and fruit weight were established. Principal component analysis grouped variables into three components which explain 69.89% of the total variation. Cluster analysis divided genotypes into six groups of similarities. Among the genotypes examined, they have a relatively high level of variability and their polymorphism could be used in a breeding program aimed at combining valuable characters.

Key words: phenotype, Cucumis melo, breeding program, genetic improvement

Corresponding author: Nikolay Velkov, Maritsa Vegetable Crops Research Institute, Department of Plant Breeding, 32 Brezovsko Shosse Str., 4003 Plovdiv, Bulgaria,e-mail: velkov_n@abv.bg; telephone +35932952296.

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Published in „GENETIKA“ Vol. 53, No.2 (2021), pp.559-574

Serbia

UDC 575.

https://doi.org/10.2298/GENSR2102559Y

Original scientific paper

GENETIC DIVERSITY AND RELATIONSHIPS AMONG SALVIA SPECIES

BY ISSR MARKERS

Zhao YANPENG¹, Wang HONGMEI^2*, Liang WEI³, Majid KHAYATNEZHAD⁴ and FAISAL⁵

¹Department of Fundamental Courses, Shandong University of Science and Technology, Taian, Shandong, China

²Dept. of Physical Education, University of Science and Technology Liaoning, Anshan, China

³Institute of Physical Education, Qingdao University of Science and Technology, Qingdao Shandong 266061, China

⁴Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran

⁵ Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan.

Abstract

Yanpeng Z., W. Hongmei, L. Wei, M. Khayatnezhad, Faisal (2021). Genetic diversity and relationships among Salvia species by ISSR markers. - Genetika, Vol 53, No.2, 559-574.

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

Key words: ISSR, Morphology, Species Identification, STRUCTURE

Corresponding author: Wang Hongmei, Dept. of Physical Education, University of Science and Technology Liaoning, Anshan, China, E-mail: masxiongg@163.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 575-582

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102575K
Original scientific paper

DETERMINATION OF A SEX-RELATED RAPD MARKER IN CAROB

(Ceratonia siliqua L.)

Dilek KAYA and Zeynel DALKILIÇ*

Aydın Adnan Menderes University, Faculty of Agriculture, Department of Horticulture, Aydın, Turkey

Abstract

Kaya D. and Z. Dalkiliç (2021). Determınatıon of a sex-related rapd marker ın carob (Ceratonia siliqua L.). - Genetika, Vol 53, No.2, 575-582.

Molecular markers are used in the characterization and breeding of organisms. Carob (Ceratonia siliqua L.) is a species with both dioecious and hermaphrodite flower forms. The determination of sex at an early stage of growth in this species, whose juvenility period is long, is important in breeding studies. The objective of this study was to identify the sex-related markers using RAPD method. Ten genotypes were obtained from natural F₁ hybrids between a naturally grown a female and a male carob tree. DNA was extracted from the leaves of 12 carob plants. Using BSA, the female and male bulks were formed from five female and five male plants, respectively, using equal amounts of DNA from each plant. In this study, 130 RAPD primers were tested. That of 21 primers tested showed polymorphisms between male and female bulks. While the fragment of 750 bp from the OPA17 RAPD primer was not detected in the female parent, female bulk, and female F₁ hybrids; it was observed in the male parent and four out of five male F₁ hybrids. This is the first report in the literature that one RAPD marker, namely OPA17-750, related to 80% reliability to male sex in carob was determined.

Keyword: Ceratonia siliqua, gender, molecular markers, BSA, PCR

Corresponding author: Zeynel Dalkiliç,Aydın Adnan Menderes University, Faculty of Agriculture, Department of Horticulture, South Campus 09100 Aydın, Turkey; zdalkilic@adu.edu.tr; Tel: +90-5335158159

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Published in „GENETIKA“ Vol. 53, No.2 (2021), pp. 583-591

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102583U
Original scientific paper

DEVELOPMENT OF BREEDING LINES BY ZIGOTIC OVULE CULTURE

IN Nigella sativa L.

Hüseyin UYSAL^* and Emre SEVİNDİK

Aydın Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydın, Turkey

Abstract

Uysal H. and E. Sevindik (2021). Development of breeding lines by zigotic ovule culture in Nigella sativa L. - Genetika, Vol 53, No.2, 583-591.

This study was carried out in order to develop an embryo culture technique and to establish breeding lines with this technique. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Embryo cultures were done by using populations obtained from producers in Samsun, Denizli, Isparta, Mersin in Turkey and Çameli black cumin variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. LS2.5 and MS (Sigma Aldrich Catalog number: M5519) mediums were used for ovule culture and MSD4 medium was used growing the plants obtained from the ovules. As a result of the research, a total of 2904 ovules were cultured in LS2.5 medium. 148 of them showed callus development; callus formation rate was 5.10%. The highest callus formation rate in the investigated combinations was obtained from Çameli x Denizli combination at 7.26%. Plant regeneration could not be obtained from these calluses. A total of 3526 ovules were cultured in MS medium. Sixty plantlets were obtained from these ovules and plant formation rate was determined as 1.70%. 41 plants from these matured and were harvested.

Keyword: Black cumin, embryo culture, in vitro, Nigella sativa, ovule culture

Corresponding author: Hüseyin Uysal, Aydın Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydın, Turkey, E-mail: hüseyin.uysal@adu.edu.tr, Phone: +90 256 772 7024 Ex: 6430

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 593-608

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102593U
Original scientific paper

RESPONSE OF Different Rootstocks on Vegetative Growth,

fruıt and Seed Yield of Eggplant (Solanum melongena L.)

Firdes ULAS

Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri- Turkey.

Abstract

Ulas F. (2021). Response of different rootstocks on vegetative growth, fruit and seed yield of eggplant (Solanum melongena L.). - Genetika, Vol 53, No.2, 593-608.

In this study, different eggplant scion genotypes were grafted onto different eggplant rootstock genotypes in order to assess the plant growth, development, and fruit yield of eggplant plants based on the physiological and morphological response mechanisms. The experiment was conducted at the vegetable research field plot on the campus at Faculty of Agriculture, University of Erciyes, Kayseri-Turkey. The field experiment was carried out to determine plant growth, shoot and root fresh and dry biomasses, plant height, total leaf area, leaf chlorophyll index (SPAD), rootstock and scion stem diameter, number of fruits per plant, total fruit yield, fruit dry matter, fruit diameter and length, total seed yield, and thousand seed weight. Two eggplant cultivars (Topan and Pala) were grafted onto two different eggplant rootstock genotypes (Hawk and Köksal F1), while non-grafted scion genotypes were used as control plants. The experiment was laid in a randomized completely block design (RCBD) containing three replications. The results indicated that between grafted and non-grafted plants significant (P<0.001) differences were observed in shoot and root fresh and dry biomasses, plant height, total leaf area, leaf chlorophyll index (SPAD), rootstock and scion stem diameter, number of fruits per plant, total fruit yield, fruit dry matter, fruit diameter and length, total seed yield, and thousand seed weight. The rootstocks utilized have influenced the vigour of the grafted plants. The ‘Pala’ grafted on the rootstock ‘Köksal F1’ registered 61.91% increment in shoot fresh biomass and 49.04% increment in root fresh biomass in comparison to the non-grafted plants. Grafting influenced plant height which reached values of roughly 1.0 m, leaf area resulting in values of 5645.04 cm² plant^-1, and SPAD resulting in values of 47.48 at ‘Pala/Köksal F1’ graft combination in comparison to the non-grafted plants. The grafting combinations also influenced the productivity of plants as compare to non-grafted plants. The significantly highest fruit yield obtained from ‘Pala/Köksal F1’ graft combination was of 4711.89 g plant^-1, followed by ‘Topan/Köksal F1’ graft combination with 3834.54 g plant^-1. ‘Pala’ was produced 11.49 number of fruits per plant when grafted on the ‘Köksal F1’ rootstock and 8.46 number of fruits per plant when grafted on the ‘Hawk’ rootstock. Regarding seed yield, ‘Pala’ grafted on the rootstock ‘Köksal F1’ registered 72.03% increment in total seed yield in comparison to the non-grafted plants. Overall, the eggplant rootstock genotypes ‘Köksal F1’ showed a better performance shoot and root fresh and dry biomasses, plant height, total leaf area, leaf chlorophyll index (SPAD), rootstock and scion stem diameter, number of fruits per plant, total fruit yield, fruit dry matter, fruit diameter and length, total seed yield, and thousand seed weight as compared to non-grafted plants.

Keyword: eggplant, fruit diameter, rootstock, SPAD, yield

Corresponding author: Firdes Ulas, Department of Horticulture, Faculty of Agriculture, Erciyes University, Kayseri- Turkey. E-mail: fulas@erciyes.edu.tr, Tel:+90 537 919 3185

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Published in „GENETIKA“ Vol. 53, No2 (2021), pp609-627

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102609N
Original scientific paper

SPECIES DELIMITATION IN CAPPARIS (CAPPARACEAE): MORPHOLOGICAL AND MOLECULAR

Sakine NAJAFIAN¹, Iraj MEHREGAN¹, Alireza IRANBAKHSH^1*,Mostafa ASSADI²,

Silvio FICI³

¹Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Research Institute of Forest and Rangelands, National Botanical Garden of Iran, Tehran, Iran

³Dipartmento di Science Agrarie e Forestali, University di Palermo, Palermo, Italy

Abstract

Najafian S., I.Mehregan, A. Iranbakhsh,M. Assadi, S. Fici (2021). Species delimitation in Capparis (Capparaceae): morphological and molecular. - Genetika, Vol 53, No.2, 609-627.

Capparis spinosa L. (Capparidaceae) is the largest genus of the family Capparaceae, distributed in pantropical region. C. spinosa is known as a medicinal plant species. In Iran, different parts of caper bush plants are used as diuretics, tonics and in treatment of malaria and joint disease. Till present time, there has been no detailed information available on molecular phylogeny and genetic structure of these species in the country. Therefore, the present study was conducted with the aim to investigate species delimitation by both morphological and molecular data and to reveal genetic diversity and population structure in these five of Capparis species. For this study, 108 randomly collected plants from 20 geographical populations in of Capparis species were used. We encountered extensive within species genetic and morphological diversity. ISSR molecular markers could delimit the studied species. STRUCTURE analysis revealed the occurrence gene flow between these species. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Phylogenetic tree was constructed based on ITS data set which separated out-groups from the studied species. Genetic affinity of the studied species have been discussed.

Keyword: Capparis spinosa, Genetic admixture; ISSR, ITS, Population Structure

Corresponding author: Alireza Ianbakhsh, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, E-mail: iranbakhsh@iau.ac.ir

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 629-640

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102629K
Original scientific paper

GENETIC DIVERSITY ANALYSIS THROUGH CLUSTER CONSTELLATION

IN BRINJAL (Solanum melongena L.)

Sukhninder KAUR, Mohinder Kaur SIDHU, Ajmer Singh DHATT

Department of Vegetable Science, Punjab Agricultural University, Ludhiana-141004, Punjab, India

Kaur S., M. K. Sidhu, A. S. Dhatt (2021). Genetic diversity analysis through cluster constellation in brinjal (Solanum melongena L.). - Genetika, Vol 53, No.2, 629-640.

In present investigation, 110 locally developed genotypes from different breeding programmes in brinjal were classified into eleven clusters on the basis of their D² values computed from Mahalanobis D²statistics of twelve morphological traits, wherein inter- and intra-cluster distances highlighted the genetic divergence of the genotypes grouped among and within different clusters. Among all, fourth cluster was the largest with 33 genotypes; however, each of second, fifth, ninth, tenth and eleventh clusters contained only single genotype. The genotypes of eighth and tenth clusters were highly diverse (1584.40) followed by third and eighth (1431.31), eighth and ninth (1302.69), sixth and eighth (1126.33) and first and eighth (1042.91) clusters. Intra-cluster (within cluster) variation was the highest in seventh cluster (74.43) followed by eighth (61.20) and sixth (54.36) that described the diverse nature of eighteen, five and nineteen genotypes in these groups, respectively. However, PBL-268, PBGL-401, PBL-243, PSR 308 and PBOB-518 were grouped individually in II^nd, V^th, IX^th, X^th and XI^th clusters, respectively. Overall, fifth cluster had most vigorous and high yielding ((2.82 kg/plant) genotype (PBGL-405); eighth cluster included genotypes with big round fruits and maximum fruit weight (317.43g); and tenth cluster had the earliest genotype (PSR-308) with the maximum number of fruits per plant (43.17). Out of twelve morphological traits, 94.19% diversity was brought by average fruit weight (67.86%), number of fruits per plant (17.26%), fruit yield per plant (5.37%) and fruit breadth (3.70%), however, other traits had negligible share towards the variation. This study created the foundation for future hybridization programmes in brinjal, where the parents can be selected on the basis of highly diverse groups as well as traits.

Keyword: Brinjal, morphological diversity, inter-cluster distance, Mahalanobis D²statistics

Corresponding author: Mohinder Kaur Sidhu, Punjab Agricultural University, Ludhiana-141004, Punjab, India; Phone: +919463664452, E mail: mksidhu@pau.edu

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Published in „GENETIKA“ Vol. 53, No.2(2021), pp. 641-649

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102641S

Original scientific paper

THE Morfological and Molecular identification of Fusarium verticillioides causing fusariosIs on wheat grain

Iva SAVIĆ^1*, Milica NIKOLIĆ¹, Ana NIKOLIĆ¹, Ivana VICO²,

Snežana MLADENOVIĆ DRINIĆ¹, Nataša DUDUK², Slavica STANKOVIĆ¹

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

Abstract

Savić I., M. Nikolić, A.Nikolić, I. Vico, S. Mladenović Drinić, N. Duduk, S. Stanković (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. - Genetika, Vol 53, No.2, 641-649.

During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25^oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.

Keyword: fungi, Fusarium verticillioides, identification, pathogenicity, primers, wheat

Corresponding author: Iva Savić, Maize Research Institute Zemun Polje, Belgrade, Serbia,

E-mail isavic@mrizp.rs; Phone: 0648406381

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 651-662

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102651M

Original scientific paper

GENETIC DIVERSITY OF Lonicera L. (Caprifoliaceae) ESTIMATED

BY MOLECULAR MARKERS AND MORPHOLOGICAL CHARACTERS

Xin MA^1*,Huailiang TIAN², Haiou XIA¹, ZEENAT³

¹College of Architecture, Chongqing Metropolitan College of Science and Technology, Yongchuan, Chongqing 402167, China

²Chongqing Popjoy Landscape Planning and Design Co., Ltd. Yubei Chongqing, 401120, China

³Institute of Biochemistry, University of Sindh Jamshoro, Pakistan

Abstract

Ma X.^,H. Tian, H. Xia, Zeenat (2021). Genetic diversity of Lonicera L. (caprifoliaceae) estimated by molecular markers and morphological characters. - Genetika, Vol 53, No.2, 651-662.

Members of Lonicera are characterized by opposite, narrowly elliptic to obovate leaves, white, yellow, reddish, or purple-red corolla with capitate stigma and undulate calyx margin. In Flora Iranica, Wendelbo (1965) classified 19 species of the Lonicera into two subgenera (Chamaecerasus and Lonicera) and three sections, namely Isoxylosteum, Isika and Coeloxylosteum. The four studied species belong to subgenus Chamaecerasus and sections Isika and Coeloxylosteum. No detailed Random Amplified Polymorphic DNA (RAPD) studies were conducted to study Lonicera genetic diversity. Therefore, we collected and analyzed three species from 2 provinces regions. Overall, 45 plant specimens were collected. Our aims were 1) to assess genetic diversity among Lonicera species 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. The Mantel test showed correlation (r=0.66, p=0.0001) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the Lonicera species can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in Lonicera species.

Keyword: Gene flow, Random Amplified Polymorphic DNA (RAPD), Lonicera species, isolation, morphometry

Corresponding author: Xin Ma, College of Architecture, Chongqing Metropolitan College of Science and Technology, Yongchuan, Chongqing 402167, China, E-mail: mamaxin2021@163.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 663-670

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102663P

Original scientific paper

CYTOGENETIC ASPECTS OF MISCARRIAGE

Bojana PETROVIĆ* and Milica KOMNENIĆ RADOVANOVIĆ

Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade

Abstract

Petrović B. and M. Komnenić Radovanović (2021). Cytogenetic aspects of miscarriage. - Genetika, Vol 53, No.2, 663-670.

Chromosomal aberrations account for approximately 50% of fetal losses prior to the 15^th week of gestation. The aim of this study was to determine the differences in frequencies and distribution of chromosomal aberrations in sporadic and habitual abortions. During a seven year period (2007.-2014.), we have analyzed 380 samples of chorionic villi after missed abortion in the Clinic for gynecology and obstetrics, Clinical center of Serbia. After first misscariage we analyzed 268 samples, and after habitual abortions 112 samples. For statistical analysis, we used χ² test. Karyotype analysis revealed chromosomal aberrations in 22,4% (85/380) of all samples. In the group after first abortion, we found an aberrant karyotype in 15,7% (42/268) of cases. In the group with habitual abortions, chromosomal aberrations were detected in 38,4% (43/112) of cases. Statistical analysis showed significant difference between these two groups, χ²=11,34> χ²_{(1 I
0,05)}=3,841i p<0,05. The distribution of chromosomal aberrations was similar in both groups. Also, in both groups, numerical chromosomal aberrations were the most common. The identification of cytogenetic causes is an important component in miscarriage etiology investigation, and it is recommended in order to improve genetic counseling of an involved couple.

Keyword: spontaneous abortion, habitual abortions, chromosomal aberrations

Corresponding author: Bojana Petrović, Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade, E-mail: mrdrbojaninmail@gmail.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp.671-686

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2102671M
Original scientific paper

HEAT-INDUCED ACCUMULATION OF PROLINE AND YIELD COMPONENTS

GENETIC DIVERSITY AND POPULATION STRUCTURE

OF IRANIAN PISTACHIO (Pistacia vera L.) CULTIVARS

Mojdeh MAHDAVI¹, Fariba SHARIFNIA^1,*, Fahimeh SALIMPOUR¹,

Akbar ESMAEILI², Mohaddeseh LARYPOOR³

¹Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran

²Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran

³Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Mahdavi M., F. Sharifnia, F.Salimpour, A. Esmaeili, M. Larypoor (2021). Genetic diversity and population structure of Iranian pistachio (Pistacia vera L.) cultivars. - Genetika, Vol 53, No.2, 671-686.

Iran has a rich pistachio germplasm, thereby, the diversity and number of Iranian pistachio cultivars is unique in the world. Genetic diversity is crucial for sustainable use of genetic resources and conservation. As one of the oldest nut crops in human history, pistachio nuts have a high nutritional value and are commercially important. In the present study, the genetic variation of pistachio genotypes was investigated by nuclear ISSR markers. In this study, genetic relationships among 11 cultivars was assessed by using 12 inter simple sequence repeat (ISSR) primers. The total of 53 bands of which 44 (83%) were polymorphic were amplified by the 12 primers, an average of 4.4 bands per primer. The total number of amplified fragments was between 2 to 6 and the number of polymorphic fragments ranged from two to six. The amplified allele sizes ranged from 300 to 1600 bp. Pair-wise genetic similarity coefficients varied from 0.70 to 0.95. The UPGMA dendrogram differentiated the genotypes into two major clusters. The Mantel test showed correlation between genetic and geographical distance. AMOVA revealed a significant genetic difference among cultivars and showed that 35% of total genetic variation was due to within- cultivars diversity. The present results may be used for the conservation, core collection and future breeding of the pistachio.

Keyword: Gene flow, Genetic admixture, Network, Pistacia vera; genetic diversity

Corresponding author: Fariba Sharifnia, Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran, E-mail: fa.sharifnia@gmail.com, f_sharifnia@iau_tnb.ac.ir

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp687-702

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2102687B
Original scientific paper

GENOTYPIC VARIABILITY OF ROOT AND SHOOT TRAITS OF BREAD WHEAT (Triticum aestivum L.) AT SEEDLING STAGE

Milica BLAŽIĆ¹, Dejan DODIG², Vesna KANDIĆ², Dragoslav ĐOKIĆ³, Tomislav ŽIVANOVIĆ⁴

¹Technical College of Vocational Studies at Požarevac, Požarevac, Serbia

²Maize Research Institute, Zemun Polje, Belgrade, Serbia

³Faculty of agronomy, University of Nis, Kruševac, Serbia

4Department of Genetics, Plant Breeding and Seed Production, Faculty of Agriculture, University of Belgrade

Abstract

Blažić M., D. Dodig, V. Kandić, D. Đokić, T. Živanović (2021). Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage. - Genetika, Vol 53, No.2, 687-702.

The evaluation of the embryonic root and stem of bread wheat (Triticum aestivum L.) in the early stage of development (seedling stage) can be a powerful tool in wheat breeding aimed at obtaining progenies with a greater early vigour. It is revealed that genotypes with faster early vigour have produced higher biomass and grain yield. In this study, the evaluation of traits of the embryonic root and the embryonic stem of 101 bread wheat genotypes was preformed at the 10-day old seedlings. The following eight morphological traits of roots and stems were analysed: primary root length, branching interval, the number of roots, total length of lateral roots, angle of seminal roots, stem length, root dry weight and the stem dry weight. Analysed lateral roots included seminal roots. The greatest, i.e. the smallest variability of observed traits was detected in the branching interval, i.e. the stem length, respectively. The highest positive correlation was determined between the primary root length and the total length of lateral roots. The cluster analysis, based on observed traits, shows that genotypes were clearly divided into two main clusters, A and B. The two clusters essentially differed from each other in the values of the following traits: primary root length, total length of lateral roots, root dry weight, stem dry weight and the stem length. Genotypes with shorter primary and lateral roots, lower root and stem dry weight and a shorter stem were grouped in the cluster B. On the other hand, the cluster A encompassed genotypes with values of these traits above or around the average. The values of the remaining analysed traits: the angle of seminal roots, the number of lateral roots and the branching interval varied greatly between obtained clusters. The cluster analysis showed the homogeneity of genotypes originating from Serbia and the region; their values of the root and stem length and weight were mostly around and below the average. However, the values of the angle of seminal roots, number of lateral roots and the branching interval were above average.

Keyword: wheat, root, stem, correlations, claster analysis

Corresponding author: Milica Blažić, Technical College of Vocational Studies at Požarevac, Nemanjina 2, 12000 Požarevac, Serbia, E-mail: milica.blazic@vts-pozarevac.edu.rs, Telephone: +38163326500.

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 703-716

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102703W
Original scientific paper

EXPRESSION OF CAVEOLIN-2 IN PATIENTS WITH ORAL CANCER

AND CORRELATIONS WITH CLINICOPATHOLOGICAL PARAMETERS

Chao WAN†, Fang ZHANG†, Liangming ZHU*

Department of Oral and Maxillofacial Surgery, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, P. R. China

†The two authors contributed equally to this study.

Abstract

Wan C., F. Zhang, L. Zhu (2021). Expression of caveolin-2 in patients with oral cancer and correlations with clinicopathological parameters. - Genetika, Vol 53, No.2, 703-716.

We aimed to analyze the expression of caveolin-2 (CAV2) in patients with oral cancer and its correlations with clinicopathological parameters. The expression of CAV2 in oral cancer and its influence on the survival curves of oral cancer patients were inquired through the Human Protein Atlas Database. The cancer tissue specimens and normal paracancerous tissue specimens (≥2 cm away from cancer tissues) were collected from 173 patients with oral cancer confirmed by pathology. Moreover, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to detect the messenger ribonucleic acid (mRNA) and protein expressions of CAV2 in oral cancer tissues and corresponding paracancerous tissues, respectively, and their associations with the clinicopathological characteristics and survival conditions of oral cancer patients were analyzed. It was shown in the Human Protein Atlas Database that the expression of CAV2 was increased significantly in oral cancer tissues compared with that in normal tissues (P<0.05), and patients with a low expression of CAV2 had a longer survival time than those with a high expression of CAV2 (P<0.05). The results of qRT-PCR and immunohistochemistry manifested that the mRNA expression level of CAV2 and the percentage of CAV2-positive cells were significantly higher in oral cancer tissues than those in paracancerous tissues (P<0.05). The CAV2 expression was correlated with clinical stage and pathological differentiation degree (P<0.05). In comparison with those in patients with a low CAV2 expression, the overall survival (OS) curve, relapse-free survival (RFS) curve and survival rate declined significantly in patients with a high CAV2 expression (P=0.001). Besides, the CAV2 expression, clinical stage and pathological differentiation degree were independent influencing factors for the postoperative OS and RFS of patients. The expression of CAV2 had relatively high predictive value for the OS and RFS of patients with oral cancer within 5 years after operation, of which the area under curve was 0.827 and 0.874, and the optimal cut-off value was 27.97% and 32.84%, respectively. CAV2 is highly expressed in oral cancer. With rising CAV2 expression level, the survival time of patients is shortened and the relapse risk is elevated, suggesting a poor prognosis.

Keyword: caveolin-2, oral cancer, clinicopathological parameter

Corresponding author: Liangming Zhu, Department of Oral and Maxillofacial Surgery, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, P. R. China, E-mail: carlorussohri@yahoo.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 717-727

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102717D
Original scientific paper

COMPOSITIONAL DIVERSITY IN ESSENTIAL OIL

OF Ziziphora tenuior L. ECOTYPES

Hulya DOGAN

Yozgat Vocational School, Plant and Animal Production, Yozgat Bozok University,

Yozgat, Turkey

Abstract

Dogan H. (2021). Compositional diversity in essential oil of Ziziphora tenuior L. ecotypes. - Genetika, Vol 53, No.2, 717-727.

Present study reports a comparative screening of three ecotypes of Ziziphora tenuior L., sampled from three region with different elevation in middle and northeastern Turkey, based on their essential oil composition, total phenolic content and antioxidant capacities. Z. tenuior ecotypes showed twenty compounds, representing 89.18-91.44% of the essential oil (EOs) identified by GC-MS. The major components of EOs in ecotypes were pulegone (37.23-49.12%), and followed by 1,8-Cineole (2.26-7.78%), limonene (4.20-5.44%), β-Caryophyllene (3.88-5.11%) and thymol (2.68-4.11%), respectively. Ecotypes showed variable pulegone content and it was increased with elevation increase. The total phenolic contents varied among ecotypes ranged from 21.13 to 27.50 mg of gallic acid equivalent/g EOs. Antioxidant capacity was determined in vitro using DPPH radical-scavenging and FRAP (Ferric reducing power) assays and expressed as concentration of each extract required to inhibit radical by 50% (IC₅₀and EC₅₀) values that ranged from 0.80 to 0.97 mg/ml in DPPH and 0.42 to 0.55 mg/ml in FRAP assays, respectively. Our results indicated that antioxidant capacity changed among ecotypes. Taking into account the essential oil content, it seems that to select ecotype was the most appropriate to obtain more pulegone for this plant from its wild habitat.

Keyword: Essential oils, genetic background, ecotype, biological activity, Ziziphora tenuior L.

Corresponding author: Hulya Dogan, Yozgat Vocational School, Plant and Animal Production, Yozgat Bozok University, Yozgat, Turkey, E-mail: hulya.dogan2026@gmail.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 729-737

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102729V
Original scientific article

ASSOCIATION STUDY OF RS535296987 IN TEX14 GENE IN AZOOSPERMIAMEN: RFLP AND DNA SEQUENCING

Mohammad VAHEDI¹and Masoud SHEIDAI²

¹Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran

²Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology,

Shahid Beheshti University, Tehran, Iran

Abstract

Vahedi M.and M. Sheidai (2021). Association study of rs535296987 in tex14 gene in azoospermiamen: RFLP and DNA sequencing. - Genetika, Vol 53, No.2, 729-737.

Azoospermia is one of the kinds of male infertility, with clinically the most severe phenotype as the natural conception cannot occur. It has been estimated to affect 0.1 to 1% of all men and 10-15% of men in infertile couples. TEX14 (Testis expressed 14, intercellular bridge forming factor) is a protein coding gene, which is located in human chromosome 17, (17q22). Tex14 gene appears to be crucial for perfect spermatogenesis and functional studies indicate the role of TEX14 in the intercellular bridges between developing male germ cells. The gene contains 32 exons and spans 137 kb. A heterogeneousresultis available on the association TEX14 gene and azoospermia. Therefore, it is suggested to investigate this gene in different populations. We analyzed about 200 men in two categories of azoospermia and healthy persons by RFLP as well as DNA sequencing to indicate an association between rs535296987 in TEX14 and its adjacent nucleotides to azoospermia. We found no significant association based on RFLP data and also by clustering of case and control specimens based on DNA sequencing. In general, a low level of nucleotide variability was observed in DNA sequences. Therefore, both eternity in the studied samples and low degree of mutations in this genetic region, may be the reason for heterogeneous reports on association of TEX14 and azoospermia.

Keyword: association, Azoospermia, rs535296987, TEX14

Corresponding author: Masoud Sheidai, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University, Tehran, Iran, e-mail: msheidai@yahoo.com

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Published in „GENETIKA“ Vol. 53, No2(2021), pp.739-756

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102739L
Original scientific paper

SCOT MOLECULAR MARKERS AND POPULATION DIFFERENTIATION

IN Hedera helix L.

Haiying LI^1,*, Yingxue WANG¹ and Rabia IQBAL ²

¹North China University of Water Resources and Electric Power, Zhengzhou, Henan, P.R.China

²Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan

Abstract

Li H., Y. Wang, R, Iqbal (2021). SCoT molecular markers and population differentiation in Hedera helix L.. - Genetika, Vol 53, No.2, 739-756.

Hedera helix L. is a specie that is used for its ornamental and medicinal properties widely. In spite of its very good biochemical characterization, the knowledge about the DNA variability is very limited and no DNA markers were used to analyses the genomic variability of the populations, up to date. In the present study, genetic diversity of 56 Hedera helix, individuals nine populations were studied using 10 Start Codon Targeted (SCoT) markers. High polymorphic bands (95.78%), polymorphic information content (0.25) and allele number (1.34) showed SCoT as a reliable marker system for genetic analysis in Hedera helix. At the species, the percentage of polymorphic loci [P] was 66.20%, Nei’s gene diversity [H] was 0.159, Shannon index [I] was 0.148 and unbiased gene diversity [UHe] was 0.56. Genetic variation within populations (70%) was higher than among populations (30%) based on analysis of molecular variance (AMOVA). We used SCoT molecular marker for our genetic investigation with the following aims: 1— Investigate genetic diversity both among and with date Hedera helix, 2—Identify genetic groups within these nine populations ivy, and 3—produce data on the genetic structure of date ivy populations. The results obtained revealed a high within-population genetic variability.

Keyword: Genetic admixture, Gene flow, Genetic structure, SCoT, Hedera helix L.

Corresponding author: Haiying Li, North China University of Water Resources and Electric Power, No.136 Jinshui east road, Zhengzhou, Henan, P.R.China. E-mail: lhy9870654@126.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 757-768

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102757B
Original scientific paper

GENETIC DIVERSITY ANALYSIS OF THE MEDICINAL PLANT Heracleum persicum Desf. ex Fischer

Faegheh BEHZADIFAR, Sayeh Jafari MARANDI^*, Ahmad MAJD, Fahimeh SALIMPOUR, Sedigheh ARBABIAN

Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Behzadifar F., S. J. Marandi, A. Majd, F. Salimpour, S. Arbabian (2021). Genetic diversity analysis of the medicinal plant Heracleum persicum desf. ex fischer. - Genetika, Vol 53, No.2, 757-768.

Persian hogweed (Heracleum persicum Desf. ex Fischer) is native to Iran but was introduced to Europe as an invasive plant. It has medicinal and edible values. Its fruits have been used to relieve flatulence, stomach aches as well as a flavoring as a digestive and an antiseptic. This medicinal plant forms many geographical populations in the country, particularly in mountainous regions; however, we have no data on the genetic structure and genetic diversity of this plant species. Such information can be used in gene pool identification and future genetic conservation and breeding programs. Therefore, the present study was performed with the following aims: 1- Provide data on genetic diversity of geographical populations, 2- Identify the potential gene pools for future genetic conservation and breeding programs. We used both Inter-simple sequence repeats (ISSR) and the Start Codon Targeted (SCoT) molecular markers as these are suitable molecular markers for genetic diversity investigations. There are highly polymorphic, reproducible, and relatively low cost markers. The present study revealed that both ISSR and SCoT markers produce data on the genetic variability and genetic affinity of the local populations. Both molecular markers revealed a good level of genetic variability within and among Heracleum persicum populations. Analysis of molecular variance (AMOVA) produced a significant differences between geographical populations for both markers. We could identify few ISSR as well as SCoT bands which can differentiate the studied populations. The moderate to a good level of genetic diversity we observed within each H. persicum may be due to cross pollination. In conclusion, we suggest using combination of ISSR and SCoT molecular markers to study population genetic variability in H. persicum geographical populations for future genetic conservation and germplasm collection of this medicinal plant.

Keyword: Heracleum persicum, ISSR, SCoT, genetic variability.

Corresponding author: Sayeh Jafari Marandi: Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran. E-mail, jafarisayeh@gmail.com

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Published in „GENETIKA“ Vol. 53, No. 1(2021), pp 769-782

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102769M
Original scientific paper

GENE FLOW AND GENETIC STRUCTURE BETWEEN POPULATIONS

OF Hesperis L. (BRASSICACEAE) SPECIES USING MOLECULAR MARKERS

Kuanhong MENG^1*,Jia YAO², CongYing HE¹, Hakimeh MORABBI HERAVI³

¹Ningbo Institue of Oceanography Ningbo, Zhejiang, 315832, China

²School of Civil Engineering and Architecture, NingboTech University Ningbo, Zhejiang, 315100, China

³Department of Statistics, University of Bojnord, Iran

Abstract

Meng K., J. Yao, C.Y. He and H. Morabbi Heravi (2021). Gene flow and genetic structure between populations of Hesperis L. (Brassicaceae) species using molecular markers - Genetika, Vol 53, No.2, 769-782.

Genetic variability and populations^,structure were studied in seven geographical populations of Hesperis L. Genetic diversity parameters were determined in these populations. 5 of 10 random amplified polymorphic DNA primers produced 62 reproducible bands with average of 7.1 bands per primer and 55% of polymorphism. Hesperis hyrcana showed the highest number of effective allele (Ne), Shannon index (I) and genetic diversity (H). The highest values of genetic diversity were obtained in Hesperis hyrcana. NJ trees grouped the populations in two different clusters/groups, indicating their genetic difference which is discussed in details. The results of this study showed that the level of genetic variation in Hesperis is relatively high. NJ-based dendrogram showed a close relationship between members of Hesperis straussii and Hesperis hyrcana while the Hesperis luristanica protected population differ the most from the other populations. Principal component analysis, however, showed some minor differences with NJ-based dendrograms.

Keyword: Genetic variability, Gene flow, Hesperis; Random Amplified Polymorphic DNA.

Corresponding author: Kuanhong Meng, Ningbo Institue of Oceanography Ningbo, Zhejiang, 315832, China, E-mail: guxing_ge@163.com

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Published in „GENETIKA“ Vol. 53, No.2(2021), pp. 783-798

UDC 575.630
https://doi.org/10.2298/GENSR2102783Q
Original scientific paper

DETECTING DNA POLYMORPHISM AND GENETIC DIVERSITY

IN A WIDE PISTACHIO GERMPLASM BY RAPD MARKERS

Xiaohui QIAN¹* and Shahram MEHRI²

¹Department of Tourism and Culture, Anhui Finance and Trade Vocational College,

Hefei , China

²Department of Agronomy and Plant Breeding, ParsAbad Moghan Branch, Islamic Azad University, ParsAbad Moghan, Iran

Abstract

Qian X. and S. Mehri (2021). Detecting DNA polymorphism and genetic diversity in a wide pistachio germplasm by RAPD markers- Genetika, Vol 53, No.2, 783-798.

Assessing the genetic diversity in the population is the prerequisite to start and develop plant breeding projects. Pistacia vera is considered as a commercial species of Pistacia genus. In Iran, Pistachio export is in the second place in terms of non-oil exports and in the first place among horticultural crops. Therefore, we collected and analyzed 11 pistachio genotype (Pistacia vera), from two provinces of Iran regions. Our aims were 1) to assess genetic diversity among some of Irainian pistachio cultivars 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. Akbari cultivars depicted unbiased expected heterozygosity (UHe) in the range of 0.028. Shannon information was high (0.49) in Seifadini cultivars. Akbari cultivars howed the lowest value, 0.029. The observed number of alleles (Na) ranged from 0.261 to 2.700 in Shahpasand cultivars and Kalehghoochi cultivars. The effective number of alleles (Ne) was in the range of 1.021-1.800 for Akbari cultivars and Moosaabadi cultivars .Gene flow (Nm) was relatively low (0.38) in pistachio cultivars. The Mantel test showed correlation (r = 0.33, p=0.0001) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the among some of Irainian pistachio cultivars can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in pistachio cultivars.

Keyword: Gene flow, Random Amplified Polymorphic DNA (RAPD), pistachio cultivars, isolation, morphometry

Corresponding author: Xiaohui Qian, Department of Tourism and Culture, Anhui Finance and Trade Vocational College, Hefei, China, E-mail: xuanxuanww2@163.com; sh.mehri2000@gmail.com

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Published in „GENETIKA“ Vol. 53, No. 2(2021), pp. 799-812

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102799C
Original scientific paper

GENE FLOW AND POPULATION STRUCTURE IN ALLOCHRUSA (CARYOPHYLLOIDEAE, CARYOPHYLLACEAE) WITH THE USE OF MOLECULAR MARKERS

Weimiao CHEN¹*, Majid KHAYATNEZHAD²and Nima SARHADI³

¹College of Chinese Language and Culture, Jinan University,Guangzhou, 510610, China
²Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran

³Ph.D student of Department of Agrotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

Abstract

Chen W., M. Khayatnezhad, N, Sarhadi (2021). Gene flow and population structure in Allochrusa (Caryophylloideae, caryophyllaceae) with the use of molecular markers- Genetika, Vol 53, No.2, 799-812.

Allochrusa Bunge is a genus of subfamily Caryophylloideae Rabeler & Bittrich contains about 8 species distributed in Turkey, Central Asia, Afghanistan, Caucasus, Transcaucasus and Iran. Three species of Allochrusa versicolor, A. bungei and A. persica occur in Iran show some degree of morphological overlaps that make the species delimitation difficult. Till present time, there has been no detailed information available on morphological and genetic structure of these species in the country. The aims of the present study are: 1) to find the diagnostic value of SCoT markers in delimitation of Allochrusa species, 2) to find the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship. For this study, 97 randomly collected plants from 7 geographical populations in three Allochrusa species were used. We encountered extensive within species genetic and morphological diversity. SCoT molecular markers could delimit the studied species. AMOVA and STRUCTURE analysis revealed that the species of Allochrusa are genetically differentiated. The Mantel test showed correlation between genetic distance and geographical distance of the populations studied. Genetic affinity of the studied species has been discussed.

Keyword: Allochrusa, Endemics, Population structure, SCoT markers, Species delimitation

Corresponding author: Weimiao Chen, College of Chinese Language and Culture, Jinan University,Guangzhou, 510610, China

E-mail: weimaochen@yeah.net; majidkhayatnezhad126@gmail.com

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Published in „GENETIKA“ Vol. 53, No.2(2021), pp. 813-823

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2102813R
Original scientific paper

QUESTIONNAIRE FOR EVALUATING INFORMATION, KNOWLEDGE,

AND ATTITUDES ON DONATION, STORAGE, AND APPLICATION OF INDUCED PLURIPOTENT STEM CELLS

Sanja RAŠČANIN^1*, Mirjana JOVANOVIĆ^1,2, Dejan STEVANOVIĆ³, Nemanja RANČIĆ^4,5

¹ Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

² Psychiatric Clinic, Clinical Center "Kragujevac", Kragujevac, Serbia

³Clinic for Neurology and Psychiatry for Children and Youth, Belgrade, Serbia

⁴ Center for Clinical Pharmacology, Military Medical Academy, Belgrade, Serbia

⁵ Faculty of Medicine of the Military Medical Academy, University of Defence, Belgrade, Serbia

Abstract

Raščanin S., M.Jovanović, D. Stevanović, N. Rančić (2021). Questionnaire for evaluating information, knowledge, and attitudes on donation, storage, and application of induced pluripotent stem cells.- Genetika, Vol 53, No.2, 813-823.

The discovery of Induced Pluripotent Stem Cells (iPSCs) opened the possibilities for reprogramming adult somatic cells back to a pluripotent state in vitro by inducing a forced expression of specific transcription factors. Thus, iPSCs might have potential application in regenerative medicine, transplantation, avoidance of tissue rejection, disease modeling, and drug testing. Because of apparent ethical issues connected with donation and derivation of biomaterials, iPSCs are considered as a research alternative to ethically highly disputed Embryonic Stem Cells (ESCs). Objective: The aim of this paper was to describe the development of a questionnaire for evaluating information, knowledge, and attitudes on donation, storage, and application of iPSCs (i.e., the QIPSC). We performed a prospective qualitative study based on the development, validation and reliability testing of the QIPSC. The study included 122 respondents and the final version of the QIPSC with 34 items. The reliability analysis for part of information and knowledge of respondents according to iPSCs was then performed with the questions included in this two-component model and obtained a Cronbach's alpha value of 0.783 and 0.870, respectively. It has been shown that the range of correct answers to questions in part of knowledge of respondents according to iPSCs was from 17.2-63.1%. The results of our study show that the QIPSC was a unique, reliable, and valid questionnaire for assessing the level of information, knowledge, and attitudes on donation, storage, and application of iPSCs.

Keyword: Questionnaires, iPSCs, Validity, Reliability, Validation Studies

Corresponding author: Sanja Raščanin, Faculty of Medical Sciences, University of Kragujevac, Tel:060-0500956, E-mail: rsanja.eko@gmail.com.

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Published in „GENETIKA“ Vol. 53 No. 2(2021), pp. 825-849

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102825K
Original scientific paper

DELINEATING MULTIVARIATE DIVERGENCE, HERITABILITY, TRAIT ASSOCIATION AND IDENTIFICATION OF SUPERIOR OMEGA-3-FATTY ACID SPECIFIC GENOTYPES IN LINSEED (Linum usitatissimum L.)

Mithlesh KUMAR*, Manubhai PATEL, Ravindrasingh CHAUHAN, Chandresh TANK, Satyanarayan SOLANKI

Department of Genetics & Plant Breeding, C. P. College of Agriculture, S.D. Agricultural University, Sardarkrushinagar-385 506 (Gujarat), India

Abstract

Kumar M., M. Patel, R. Chauhan, C. Tank, S. Solanki (2021). Delineating multivariate divergence, heritability, trait association and identification of superior omega-3-fatty acid specific genotypes in linseed (Linum usitatissimum L.).- Genetika, Vol 53, No.2, 825-836.

Present investigation was undertaken to evaluate 50 linseed genotypes for three consecutive years for seed yield, oil content and agro-morphological traits using multivariate approach. Higher range, large value of Shannon-weaver diversity index for both traits and genotypes and large differences in mean values for most of the characters showed that a wide and significant variation existed among the genotypes and traits. Pooled analysis of variance revealed highly significant differences (p<0.001) among the genotypes for all the characters studied. The magnitude of the phenotypic coefficient of variation was somewhat higher than the genotypic coefficient of variation, indicating that the environment had little impact on the expression of these traits. Cluster analysis for yield and agro-morphological traits using unweighted pair group method of arithmetic averages (UPGMA) grouped the genotypes into nine clusters with varied number. Clustering of linseed genotypes from different geographical locations or source/origin into same cluster has confirmed that they are genetically related, and possibly from the same progenitor. The principal component analysis (PCA) revealed that most of the variation (76.41%) was accounted by first four PCA and indicated role of traits that contributed significantly towards a wide variation among the genotypes. The positive associations of seed yield per plant and oil content with component trait implies that improving one or more component traits could result in genetic enhancement of seed yield and oil content in linseed. The significant negative association of seed yield per plant and oil content with days to flowering and days to maturity has great advantages in breeding short duration linseed cultivars for hot and water stress climatic conditions of semi-arid regions. Trait specific genotypes namely, Shival, Sharda, IC54970, Mukta, IC56363, T-397, IC53281 and RLC-92 were identified for the development of short duration and dwarf cultivars with higher omega-3-fatty acid content.

Keyword: Multivariate approach, phenotypic diversity, linseed, omega-3-fatty acid

Corresponding author: Mithlesh Kumar, Department of Genetics & Plant Breeding, C. P. College of Agriculture, S.D. Agricultural University, Sardarkrushinagar-385 506 (Gujarat), India,E-mail: mithleshgenetix@gmail.com, Mob No: 9737555950

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Published in „GENETIKA“ Vol. 53, No.2(2021), pp. 851-865

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102851F

Original scientific paper

PALYNO-MORPHOLOGY OF Hedera L. (the ivy genus, Araliaceae) AND THEIR SYSTEMATICS IMPLICATIONS

Fahimeh FALLAH and Farrokh GHAHREMANINEJAD*

Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code 15719-14911, Tehran, Iran

Abstract

Fallah F. and F. Ghahremaninejad (2021). Palyno-morphology of Hedera L. (the ivy genus, Araliaceae) and their systematics implications- Genetika, Vol 53, No.2, 851-865.

The palynomorphological characteristics of 5 species belonging to Hedera of the Araliaceae family were studied in detail. These plant species were collected from various phytogeographical regions of Iran and Hungary. The palynological investigation was accomplished using Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Pollen grains of the species are reticulate, prolate, sub-prolate, tricolporate, isopolar, radially symmetrical, and monad. The largest pollen grain was in the Hedera colchica species (72.24±1.6 μm) and the smallest pollen grain was in Hedera helix (28.63±2.1 μm). The research carried out by the TEM showed that the species were different in terms of exine thickness, tectum thickness, foot layer thickness, the diameter and length of the Columella, the thickness and shape of the Caput, the tectum to foot layer) T/F( ratio, the absence or presence of the Endexine and the thickness of the Intine layers. The main purpose of this study was the importance of the relationship between pollen grain size and the number of chromosomes and ploidy level in Hedera species.

Keyword: Pollen Morphology, Systematics, TEM, SEM, Hedera

Corresponding author: Farrokh Ghahremaninejad, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code 15719-14911, Tehran, Iran. E-mail: fgh@khu.ac.ir.

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Published in „GENETIKA“Vol. 53, No.2(2021), pp. 867-882

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102867H

Original scientific paper

POPULATION GENETIC STRUCTURE AND GENE FLOW IN

Alcea aucheri (BOISS.) ALEF.: A POTENTIAL MEDICINAL PLANT

Liu hang¹, Li pan^1,*, Tang yong¹, Luo jianguo¹, Xu XINGMIN¹ and FAISAL²

¹School of mechanical and electrical engineering, North China institute of science & technology, (Hebei Key Laboratory of Safety Monitoring of Mining Equipment), Langfang City, Hebei Province, 065201, China

²Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan.

Abstract

Hang L., L. Pan, T. Yong, L. Jianguo, X. Xingmin, Faisal (2021). Population genetic structure and gene flow in Alcea aucheri (boiss.) Alef.: a potential medicinal plant- Genetika, Vol 53, No.2, 867-882.

The genus Alcea, a member of Malvaceae family consists of approximately 75 species worldwide distributing mainly in South-West Asia. Among these, 33 species grow in Iran. Plants of the Alcea (or Althaea) are among important medicinal plants in Iranian traditional medicine. They have long been used in the treatment of health problems and diseases. Alcea aucheri (Boiss.) Alef. species are distributed in different habitats of Iran. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Therefore, due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 118 randomly collected plants from 10 geographical populations in 5 provinces. AMOVA test revealed significant genetic difference among the studied populations and also revealed that, 74% of total genetic variability was due to within population diversity while, 26% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses revealed some degree of gene flow among these populations.

Keyword: Alcea aucheri, Gene flow, Genetic differentiation, Inter Simple Sequence Repeats (ISSR).

Corresponding author: Li Pan, School of mechanical and electrical engineering, North China institute of science & technology, Langfang City, Hebei Province, 065201, China

Email: hhxiaoxiaohh21@163.com; nasar.nas1990@gmail.com

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Published in „GENETIKA“Vol. 53, No.2(2021), pp. 883-894

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2102883Z

Original scientific paper

GENETIC RESPONSE OF GROWTH PHASES FOR ABIOTIC ENVIRONMENTAL STRESS STRONG GENETIC DIFFERENTIATION OF THE Paracaryum SPECIES (Boraginaceae) DETECTED BY INTER-SIMPLE SEQUENCE REPEATS (ISSR)

Xinxin ZHANG¹^* and Abdul SHAKOOR²

¹College of Life Science and Technology, Harbin Normal University, Harbin, China, 150025.

²Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan

Abstract

Zhang X. and A. Shakoor (2021). Strong genetic differentiation of the Paracaryum species (Boraginaceae) detected by inter-simple sequence repeats (ISSR).- Genetika, Vol 53, No.2, 883-894.

Species identification is fundamentally important within the fields of biology, biogeography, ecology and conservation. The genus Paracaryum belongs to tribe Cynoglosseae of the family Boraginaceae is a herbaceous genus including approximately 67 species, mostly distributed in the Irano-Turanian phytogeographical region. In spite vast distribution of many Paracaryum species that grow in different habitats, there are not any available report on their genetic diversity, mode of divergence and patterns of dispersal. Therefore, we performed molecular (ISSR markers) of 98 accessions from 12 species of Paracaryum that were collected from different habitats. A set of 10 ISSR markers was used. The genetic distances were estimated based on Jaccard similarity coefficient and the descriptive statistics of populations for estimation of genetic parameters were also performed. A total of 90 polymorphic bands were obtained. The present study revealed that ISSR data can delimit the species. AMOVA and STRUCTURE analysis revealed that the species of Paracaryum belongs are genetically differentiated but have some degree of shared common alleles.

Keyword: ISSR, Species Identification, STRUCTURE

Corresponding author: Xinxin Zhang，College of Life Science and Technology, Harbin Normal University, Harbin, China, 150025, E-mail: alanzhangxinxin@163.com

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Published in „GENETIKA“Vol. 53, No.2(2021), pp. 895-910

Serbia

UDC 575.630
Shttps://doi.org/10.2298/GENSR2102895L

Original scientific paper

STUDY ON GENETIC DIVERSITY BETWEEN Malva L. (Malvaceae): A HIGH VALUE MEDICINAL PLANT USING SCOT MOLECULAR MARKERS

Huixing LI^1,*, Haiyan YU¹, Xiancai ZENG¹, Sahar HUSSEIN HAMARASHID²

¹School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, Henan, China

²Sulaimany polytechnic University - College of applied science

Abstract

Li H., H. Yu, X. Zeng, S. Hussein Hamarashid (2021). Study on genetic diversity between Malva L. (Malvaceae): a high value medicinal plant using scot molecular markers.- Genetika, Vol 53, No.2, 895-910

The Malva L. genus, popularly known as mallow, grows spontaneously in almost all of Europe and the Mediterranean region. The Malva genus has 25-40 species and it can be considered as an annual and/or biannual herb. This genus contains species that reveal therapeutic properties and are mostly important in medicine and the functional food industry. Therefore, due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 97 randomly collected plants from seven species in 7 provinces. According to the SCoT markers analysis, Malva sylvestris and Malva aegyptia had the lowest similarity and the species of Malva neglecta and Malva vericillata had the highest similarity. The aims of present study are: 1) can SCoT markers identify Malva species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that combination of morphological and SCoT data can identify the species.

Keyword: Morphology, Malva; Medicinal Plants; Species identification, SCoT (Start Codon Targeted)

Corresponding author: Huixing Li, School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, Henan, China, Email:li20210702@163.com; ldongmei870@gmail.com

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Published in „GENETIKA“Vol. 53, No.2(2021), pp. 911-925

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2102911M

Original scientific paper

EFFECTS OF GENOTYPE AND BRADYRHIZOBIUM INOCULATION ON MORPHOLOGICAL TRAITS, GRAIN YIELD AND PROTEIN CONTENT OF SOYBEAN VARIETIES

Vladimir MILADINOVIĆ¹, Stefan KOLAŠINAC^1*, Ilinka PEĆINAR¹, Biljana KIPROVSKI², Dragosav MUTAVDŽIĆ³, Vladan UGRENOVIC⁴, Zora DAJIĆ STEVANOVIĆ¹

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

² University of Novi Sad, Faculty of Agriculture, Department of Field and Vegetable Crops, Novi Sad, Serbia

³ Institute for Multidisciplinary Research, University of Belgrade, Serbia

⁴Institute for Soil Science, Belgrade, Serbia

Abstract

Miladinović V., S. Kolašinac, I. Pećinar, B. Kiprovski, D. Mutavdžić, V. Ugrenovic, Z. Dajić Stevanović (2021). Effects of genotype and Bradyrhizobium inoculation on morphological traits, grain yield and protein content of soybean varieties.- Genetika, Vol 53, No.2, 911-925.

Soybean crop production in Serbia involves seed inoculation by N-fixing bacteria just before sowing time. The main objective of the current work was to assess the impact of the genotype and inoculation on range of morphological and yield traits of soybean (Glycine max L. Merrill), as well as the total protein content. The experiment was conducted on chernozem soil, where soybean was previously grown. The six local varieties were used, where each variety was sown, in three replicates for both inoculated and non-inoculated treatment. The following morphological traits were analysed: the plant height, number of lateral branches, distance to the first pod, number of pods per plant, pods (containing seeds) weight per plant, seed weight per plant, and the total grain yield. The total protein content in seeds was determined by standard analytical method, while subtle differences in qualitative protein composition were assessed using Raman spectroscopy. The total protein content varied from 39.6 to 42.15 %. Performance of inoculation resulted in an increase of the plant height and the distance to the first pod, although not in all tested varieties. The highest and the lowest plant height values were observed for non-inoculated variety Dana (59.23cm) and Sava (80.03cm), respectively. The effect of genotype was much more expressed causing differences in almost all tested characters, except for the total protein content. However, Raman spectroscopy analyses revealed distinct discrimination among surveyed varieties, and differences between inoculated and non-inoculated plants in qualitative composition of seed proteins.

Keyword: inoculation, Glycine max, varieties, yield, protein content, Raman spectroscopy

Corresponding author: Stefan Kolašinac, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia, email: stefan.kolasinac@agrif.bg.ac.rs

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Published in „GENETIKA“Vol. 53, No.2(2021), pp.927-939

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2102837M

Original scientific paper

STUDY ON GENETIC DIVERSITY BETWEEN Erodium (Geranaiceae) SPECIES BASED ON INTER-SIMPLE SEQUENCE REPEAT MARKERS

Shu-Chen MOWANG^1*, Fu-Ji CHEN¹, Zeenat²

¹School of Economics and Management Fuzhou University, Fuzhou, China

²Institute of Plant Sciences, University of Sindh, Jamshoro, Pakistan

Abstract

Mowang S.-C., F.-J. Chen, Zeenat (2021). Study on genetic diversity between erodium (Geranaiceae) species based on inter-simple sequence repeat markers- Genetika, Vol 53, No.2, 927-939.

Identifying the accurate boundaries of a species is critical to have a better perspective of any biological studies. Therefore, species delimitation is a subject of extensive part of studies in the framework of biology. Erodium species possess significant pharmacological and biological activities. The whole plant was used as astringent and haemostatic in uterine and other bleeding Therefore, due to the importance of these plant species, we performed a molecular data analysis for this species. For this study, we used 60 randomly collected plants from 5 species in five provinces. Amplification of genomic DNA using 10 primers produced 52 bands, of which 50 were polymorphic (98.48%). The obtained high average PIC and MI values revealed high capacity of ISSR primers to detect polymorphic loci among Erodium species. The genetic similarities of five collections were estimated from 0.77 to 0.91. According to Inter-Simple sequence repeats (ISSR) markers analysis, E. cicutarium and E. malacoides had the lowest similarity and the species of E. malacoides and E. oxyrrhynchum had the highest similarity. The aims of present study are: 1) can ISSR markers identify Erodium species, and 2) to investigate the species inter-relationship? The present study revealed that ISSR markers can identify the species.

Keyword: Species Identification; Structure, Erodium, ISSR markers

Corresponding author: Shu-Chen Mowang, School of Economics and Management Fuzhou University, Fuzhou, China, Email: hyzhouhai@163.com; afzalullah338@gmail.com.

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Published in „GENETIKA“Vol. 53, No.2(2021), pp.941

Serbia

https://doi.org/10.2298/GENSR2102927E

CORRIGENDUM

https://doi.org/10.2298/GENSR2102927E

Snežana Mladenović Drinić, Editor of the journal Genetika

request that it is necessary write exactly family name of the Corresponding author

POPULATION DIFFERENTIATION AND GENE FLOW IN Erodium cicutarium:

A POTENTIAL MEDICINAL PLANT

Yaocheng JIA*¹, Majid KHAYYATNEZHAD², Shahram MEHRI³

¹University of Yuncheng, Department of arts and crafts design, Yuncheng, Shanxi, 044000