GENETIKA, Vol

GENETIKA, Vol. 54, No.3 (2022)

Sudabeh BOZORGMANESH, Khalil-Berdi FOTOUHIFAR, Mohammad JAVAN-NIKKHAH, Elaheh SEIFOLLAHI

GENETIC DIVERSITY OF PLANT PATHOGEN Valsa sordida USING MICROSATELLITE MARKERS [Abstract] [Full text]

Dragan NIKOLIĆ, Zorica RANKOVIĆ-VASIĆ, Aleksandar PETROVIĆ, Saša MATIJAŠEVIĆ, Nikolina LISOV, Ivana PLAVŠIĆ

CHARACTERISTICS OF NEWLY CREATED GRAPEVINE VARIETIES VOŽD AND VLADUN [Abstract] [Full text]

Ravi Kiran Reddy KONDI, Sonali KAR, Soumya SURAKANTI

AGRO-MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION AND PRINCIPAL COMPONENT ANALYSIS FOR YIELD AND QUALITY CHARACTERS IN FINE-SCENTED RICE GENOTYPES [Abstract] [Full text]

Misko MILEV, Marinela ANGELESKA, Milena GEORGIEVA, Viktorija MAKSIMOVA, Milkica JANEVA, George MILOSHEV, Tatjana RUSKOVSKA

VITAMINS E AND C EXERT PROTECTIVE ROLES IN HYDROGEN PEROXIDE-INDUCED DNA DAMAGE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS [Abstract] [Full text]

Tijana PETROVIC, Marija ZDRAVKOVIC, Marina DJELIC, Tamara GAVRILOVIC, Zoran MIHAILOVIC, Nikola ATANASIJEVIC, Oliver STOJKOVIC

INFLUENCE OF ACE AND ACTN3 GENES POLYMORPHISMS ON CARDIOVASCULAR ADAPTATION IN FEMALE FOOTBALL PLAYERS [Abstract] [Full text]

Navin Chander GAHTYARI, Jai Prakash JAISWAL, Devender SHARMA, Mohammed TALHA, Naveen KUMAR, Narendra Kumar SINGH

GENETIC ANALYSIS AND MARKER ASSOCIATION OF PHYSIOLOGICAL TRAITS UNDER RAINFED AND HEAT STRESS CONDITIONS IN SPRING WHEAT (Triticum aestivum L.) [Abstract] [Full text]

Marko SIMONOVIĆ, Zorica LEPŠANOVIĆ, Bojan RAKONJAC_, Srđan LAZIĆ

DETECTION OF CARBAPENEM-RESISTANCE AND BIOFILM FORMATION GENES, AND GENETIC RELATEDNESS OF Acinetobacter baumannii ISOLATES [Abstract] [Full text]

Suheel AHMAD, Sheeraz Saleem BHAT, Sheikh M. SULTAN, Nazim Hamid MIR, Susheel Kumar RAINA², Natarajan SIVARAJ, Nilamani DIKSHIT, Nazir A. PALA

DIVERSITY AND ECOLOGICAL NICHE MODELLING STUDIES IN Trifolium repens L. (WHITE CLOVER) IN THE REGION OF NORTH-WESTERN HIMALAYA, INDIA[Abstract] [Full text]

Miloš Stupar, Željko Savković, Katarina Breka, Imre Krizmanić, Srđan Stamenković, Jelena Vukojević, Milica Ljaljević Grbić

New record for mycobiota of Serbia: A rare fungus Quambalaria cyanescens found in Pelophylax esculentus (Anura) skin microbiome [Abstract] [Full text]

Violeta Caro PETROVIĆ, Dragana RUŽIĆ-MUSLIĆ, Nevena MAKSIMOVIĆ, Bojana RISTANOVIC, Ivan ĆOSIĆ, Dusica OSTOJIĆ-ANDRIĆ, Dragan NIKSIĆ

PCR-RLFP ON IGFBP-3 GENE AND ITS ASSOCIATION ON GROWTH PERFORMANCE OF LAMBS REARED INTENSIVELY [Abstract] [Full text]

Maja GVOZDENOV, Branko TOMIC, Iva PRUNER, Mirjana KOVAC, Predrag MILJIC, Darko ANTIC, Valentina DJORDJEVIC

THE NOVEL FII C.*64_*66DEL PROTHROMBIN GENE VARIANT IN WOMEN WITH PREGNANCY LOSS [Abstract] [Full text]

Fatemeh NASROLLAHI, Shahrokh KAZEMPOUR-OSALOO, Valyollah MOZAFFARIAN, Hassan ZARE-MAIVAN

TEMPORAL DIVERSIFICATION IN THE GENUS ONOSMA (BORAGINACEAE) BASED ON NUCLEAR AND PLASTID DNA SEQUENCES [Abstract] [Full text]

Miroslav MIŠOVIĆ, Predrag ALEKSIĆ, Miodrag VUKOVIĆ, Dejan KOSTIĆ, Nemanja RANČIĆ, Bojana ALEKSIĆ CIKOTA

THE IMPACT OF GAS5 rs145204276 ON DEVELOPMENT AND PROGNOSIS OF PROSTATE CANCER [Abstract] [Full text]

Dragan TERZIĆ, Rade STANISAVLJEVIĆ² Tomislav ŽIVANOVIĆ, Marijenka TABAKOVIĆ, Nenad TRKULJA, Jordan MARKOVIĆ, Dobrivoj POŠTIĆ, Ratibor ŠTRBANOVIĆ

USING MOLECULAR MARKERS IN THE IDENTIFICATION OF DIFFERENT GENOTYPES OF LUCERNE (Medicago sativa L.) [Abstract] [Full text]

Hakan BAŞAK, Gölge SARIKAMIŞ, Gamze ÇAKIRER, Mevlüde Alev ATEŞ

GENETIC CHARACTERIZATION OF PEPPER (Capsicum annuum L.) GENOTYPES FROM CENTRAL ANATOLIA WITH SSR AND SCAR MARKERS [Abstract] [Full text]

Nakhshin Omer ABDULLA, Sahar Hussein HAMARASHID^,Syamand Ahmed QADIR, Aryan Mahmood FARAJ, Sherzad Rasul Abdulla TOBAKARI

GENE FLOW AND GENETIC DIVERSITY IN Consolida (Ranunculaceae) USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM [Abstract] [Full text]

Emrullah CULPAN and Burhan ARSLAN

THE GENETIC DIVERSITY OF SAFFLOWER (Carthamus tinctorius L.) GENOTYPES DEVELOPED BY HYBRIDIZATION USING SSR MARKERS [Abstract] [Full text]

Yusuf ÇİL, Ümit SERDAR, Burak AKYÜZ

SUSCEPTIBILITY LEVELS OF SOME CHESTNUT CULTIVARS AND GENOTYPES TO THE CHESTNUT GALL WASP IN TURKEY [Abstract] [Full text]

Rubina GUL, Muhammad FAHIM,Sultan Akbar JADOON, Saad Hussain SHAH, Ijaz AHMAD, Masood AHMAD

ASSESSMENT OF ELITE WHEAT GERMPLASM FOR RESISTANCE TO FUSARIUM HEAD BLIGHT -A THREAT TO WHEAT PRODUCTION IN NORTH-WEST PAKISTAN [Abstract] [Full text]

Milosav GRČAK, Dragan GRČAK, Radivoje JEVTIĆ, Mirjana LALOŠEVIĆ, Vesna ŽUPUNSKI, Branka ORBOVIĆ, Desimir KNEŽEVIĆ

VARIATION OF HARVEST INDEX OF WHEAT AND TRITICALE IN MONOCROPS AND INTERCROPS SYSTEM OF CULTIVATION [Abstract] [Full text]

Nina PETROVIĆ, Sercan ERGÜN, Marija Đorđić CRNOGORAC, Tatjana STANOJKOVIĆ, Emina MALIŠIĆ, Ivana Z. MATIĆ

Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer ceLLS [Abstract] [Full text]

Elham AMINI, Shahrokh KAZEMPOUR-OSALOO, Ali Asghar MAASSOUMI, Hassan ZARE-MAIVAN

EXPLORING THE TEMPO OF SPECIES DIVERSIFICATION IN Astragalus SECTION Incani DC. BASED ON NUCLEAR AND PLASTID DNA SEQUENCES [Abstract] [Full text]

Vesna DRAGIČEVIĆ, Milena SIMIĆ, Miodrag TOLIMIR, Nenad DJURIĆ, Milena ŠENK, Goran STANKOVIĆ, Milan BRANKOV

VARIABILITY IN ANTIOXIDANTS IN YELLOW, WHITE, AND RED COLOURED MAIZE GRAIN IN RESPONSE TO DIFFERENT FERTILIZERS [Abstract] [Full text]

Fatemeh ABDOLAHADI, Alinaghi MIRMOAYEDI, Lila ZARAEI, Samad JAMALI
GENETIC DIVERSITY STUDY OF Chrysoperla carnea (Neuroptera: Chrysopidae) POPULATIONS VIA MOLECULAR MARKERS [Abstract] [Full text]

Ana MARJANOVIĆ, Valerija DOBRIČIĆ, Milica JEČMENICA LUKIĆ, Iva STANKOVIĆ, Ognjen MILIĆEVIĆ,Nataša DRAGAŠEVIĆ MIŠKOVIĆ, Marija BRANKOVIĆ, Milena JANKOVIĆ,Ivana NOVAKOVIĆ, Marina SVETEL, Elka STEFANOVA, Vladimir KOSTIĆ

C9ORF72 REPEAT EXPANSION IS NOT ASSOCIATED WITH ATYPICAL PARKINSONISM IN THE SERBIAN POPULATION [Abstract] [Full text]

Hilal GÜZEL, Halil İbrahim GÜZEL, Cengiz ÇELEBİ, Mehmet Ali SÖZEN

ASSOCIATION ANALYSIS OF FOUR HUMAN DOPAMINE PATHWAY GENES WITH ADULT ATTENTION-DEFICIT HYPERACTIVITY DISORDER IN A POPULATION FROM TURKEY [Abstract] [Full text]

Marija BRANKOVIĆ, Elka STEFANOVA, Gorana MANDIĆ, Ana MARJANOVIĆ, Valerija DOBRIČIĆ, Aleš MAVER, Gaber BERGANT, Zorica STEVIĆ, Milena JANKOVIĆ, Ivana NOVAKOVIĆ, Borut PETERLIN, Vladimir KOSTIĆ

ANALYSIS OF “CLINICAL EXOME” PANEL IN SERBIAN PATIENTS WITH COGNITIVE DISORDERS [Abstract] [Full text]

Aleksandar KOVAČEVIĆ, Jovan PAVLOV, Milan STEVANOVIĆ, Nikola GRČIĆ, Marko MLADENOVIĆ, Nenad DELIĆ, Nemanja KNEŽEVIĆ

Effect of reciprocal crosses on grain yield and other agronomic traits in maize [Abstract] [Full text]

Vanja VIDOVIĆ, Nela MAKSIMOVIĆ, Stojko VIDOVIĆ, Tatjana DAMNJANOVIĆ, Irina MILOVAC, Ivana NOVAKOVIĆ

PPARGC1A GENE POLYMORPHISM AND ITS ASSOCIATION WITH OBESITY-RELATED METABOLIC TRAITS IN SERBIAN ADOLESCENT POPULATION [Abstract] [Full text]

Valentin Kosev, Viliana Vasileva, Vera POPOVIC, Vladan PEŠIĆ, Miloš NOŽINIĆ

Ecological-genetic model in grass pea (Lathyrus sativus L.) breeding [Abstract] [Full text]

Ege Riza KARAGUR, Mustafa Tarik ALAY, Aydin DEMİRAY, Nedim KARAGENC, Onur TOKGÜN, Taner DURAK, Hakan AKCA

THE IMPACT OF HEREDITARY THROMBOPHILIAS IN RECURRENT PREGNANCY LOSS [Abstract] [Full text]

Milica KOVAČ, Branislav KOVAČEVIĆ, Saša ORLOVIĆ

VARIABILITY OF SOME TRAITS OF WALNUTS (Juglans regia L.) IN THE TEST OF HALF SIB, ORIGINATING IN THE ĐERDAP FOREST [Abstract] [Full text]

Abdulmojeed YAKUBU, Ecevit EYDURAN, Senol CELIK, Juliana O. ISHAYA

USE OF LINEAR MODELING, MULTIVARIATE ADAPTIVE REGRESSION SPLINES AND DECISION TREES IN BODY WEIGHT PREDICTION IN GOATS [Abstract] [Full text]

Mohammad Hossein ROMENA, Abdollah NAJAPHY, Mohsen SAEIDI, Mahmud KHORAMIVAFA

GENOTYPE BY ENVIRONMENT INTERACTION ANALYSIS FOR GRAIN YIELD OF WHEAT IN IRRIGATED AND RAIN-FED MEGA-ENVIRONMENTS USING AMMI AND GGE BIPOLT MODELS [Abstract] [Full text]

Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 979-989

UDC 575.
https://doi.org/10.2298/GENSR2203979B
Original scientific article

GENETIC DIVERSITY OF PLANT PATHOGEN Valsa sordida

USING MICROSATELLITE MARKERS

Sudabeh BOZORGMANESH, Khalil-Berdi FOTOUHIFAR*, Mohammad JAVAN-NIKKHAH,

Elaheh SEIFOLLAHI

Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

Abstract

Bozorgmanesh S., KH.-B. Fotouhifar, M. Javan-Nikkhah, E. Seifollahi (2022). Genetic diversity of plant pathogen Valsa sordida using microsatellite markers. - Genetika, Vol 54, No.3, 979-989.

The genetic diversity of Valsa sordida isolates from different geographical regions and hosts was investigated using MP-PCR markers. PCR amplifications were done using eight primers. Of them, only four primers [(ATC)₇, (ACTG)₄, (CGA)₅, and (AAC)₈] produced polymorphic bands. At least 88.5% polymorphism was revealed by four primers and the maximum polymorphism (97%) was generated by (ACTG)₄ primer and three diagnosable groups (1, 2 and 3) were resolved in the resulting dendrogram constructed by the UPGMA algorithm. The results showed high polymorphism among the isolates and confirmed the merit and accuracy of the MP-PCR markers for studying the genetic variability of V. sordida isolates at the intra-species level. We have not found any correlations between observed genetic diversity and the geographical region or host plant of the isolates, unless in limited cases. The abundant formation of the sexual state of the fungus in the infected parts of trees, as well as possible asexual recombination during asexual reproduction, are suggested as influencing factors of high genetic variability among the individuals.

Key words: Cytospora chrysosperma, canker disease, tree hosts, molecular marker, MP-PCR

Corresponding author: Khalil-Berdi Fotouhifar, Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran, Telephone; 0098-26-32224022 (internal 328), Fax; 0098-26-32238529, Email; fotowhi@ut.ac.ir

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Published in „GENETIKA“ Vol. 54, No. 3 (2022), pp.991-1004

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203991N

Original scientific paper

CHARACTERISTICS OF NEWLY CREATED GRAPEVINE VARIETIES

VOŽD AND VLADUN

Dragan NIKOLIĆ^*, Zorica RANKOVIĆ-VASIĆ, Aleksandar PETROVIĆ,

Saša MATIJAŠEVIĆ, Nikolina LISOV, Ivana PLAVŠIĆ

University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

Abstract

Nikolić D., Z. Ranković-Vasić, A. Petrović, S. Matijašević, N. Lisov, I. Plavšić (2022). Characteristics of newly created grapevine varieties Vožd and Vladun. - Genetika, Vol 54, No.3, 991-1004.

This paper presents the most important morphological and production-technological characteristics of newly created grapevine varieties Vožd and Vladun, intended for the production of red wines. The newly created varieties were compared with the standard variety Cabernet Sauvignon during the three-year period of testing (2015-2017) in the relation studied properties. The variety Vožd obtained from the crossing combination Začinak ´ Prokupac, and the variety Vladun from the crossing combination Merlot ´ Župski Bojadiser. The investigated varieties differed considerably in terms of some morphological traits and represents unique genotypes. The newly recognized varieties have a hermaphrodite type of flower, dense bunch, globose berry shape and blue black color of berry skin. The yield, bunch weight, bunch length and bunch width were higher at both varieties than for the standard variety. The Vožd variety had a yield of 1.29 kg/m² and a bunch weight of 174.3 g, and the Vladun variety had a yield of 0.87 kg/m² and a bunch weight of 153.7 g. For the standard variety the grape yield was 0.78 kg/m² and the bunch weight was 134.0 g. The content of sugar and total acids in the must of the Vožd variety was 20.5% and 6.5 g/l, while for the Vladun variety it was 21.8% and 6.0 g/l respectively. Wine of both varieties was drinkable, harmonious, with a specific varietal of smell and taste and contained 12.1 vol. % of alcohol (Vožd variety), or 12.6 vol. % of alcohol (Vladun variety). Due to the many positive features of the grapes and wines from the Vožd and Vladun varieties, their spread to the production vineyards of Serbia is expected.

Key words: Vitis vinifera, hybridization, new variety, morphological traits, yield, quality

Corresponding author: Dragan Nikolić, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: +381 2615 315, fax: +381 2193 659,

e-mail: nikolicd@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 54, No.3 (2022), pp.1005-1021

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2203005K
Original scientific article

AGRO-MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION AND PRINCIPAL COMPONENT ANALYSIS FOR YIELD AND QUALITY CHARACTERS IN FINE-SCENTED RICE GENOTYPES

Ravi Kiran Reddy KONDI¹*, Sonali KAR² and Soumya SURAKANTI³

¹Division of Genetics and Plant breeding at S.G.C.A.R.S, Kumhrawand, Jagdalpur, Bastar (C.G.), Indira Gandhi Krishi Vishwavidyalaya, Raipur.

²Department of Genetics and Plant Breeding, S.G.C.A.R.S, Kumhrawand, Jagdalpur, Bastar (C.G.), Indira Gandhi Krishi Vishwavidyalaya, Raipur.

³Postgraduate, Division of Genetics and Plant breeding at B. A College of Agriculture, Anand Agricultural University, Anand, Gujarat.

Abstract

Kondi R. K. R., S. Kar, S. Surakanti (2022). Agro-morphological and biochemical characterization and principal component analysis for yield and quality characters in fine-scented rice genotypes - Genetika, Vol 54, No.3, 1005-1021.

Forty-one fine-scented rice genotypes were evaluated for 18 agro-morphological and quality characters for characterization, and 21 quantitative characters were evaluated for principal component analysis in R-studio software. Characterization of agro-morphological traits viz., plant height, days to 50% flowering, panicle length, number of effective tillers per plant, test weight, grain length, grain breadth, grain L: B ratio, kernel length, kernel breadth, kernel dimensions, awns, colour of awns, distribution of awns, and quality traits viz., alkali spreading value, gel consistency, grain aroma, and amylose content showed huge diversity among the genotypes. PCA revealed that PC1 showed the highest amount of variance (32.0%) followed by PC2 (15.7%), PC3 (9.0%), PC4 (8.1%), PC5 (7.8%), PC6 (5.4%) for quantitative characters. Out of 21 principal components, only 6 showed an eigenvalue greater than 1 and contributes about 78.1% total variance. Genotypes in PC1 showed higher values for grain L: B ratio and kernel L: B ratio. Similarly, PC2 showed higher variable values for characters like test weight, kernel length, grain length, grain breadth, alkali spreading value, grain yield per plot and amylose content. PC3 for harvest index, panicle length, gel consistency, no. of effective tillers per plant and head rice recovery. PC4 for characters like plant height, kernel breadth and days to 50% flowering. PC5 for characters like kernel elongation ratio, and filled grains per panicle. PC6 for characters like no. of tillers in a square meter and no. of panicles in a square meter. This pre-breeding characterization study may be useful in finding potential genotypes which are having both yield and quality characters which may be useful in breeding for high-yielding varieties with good-quality characters.

Keywords: Characterization, Fine-scented rice, Principal component analysis, RCBD

Corresponding author: Ravi Kiran Reddy Kondi, Division of Genetics and Plant breeding at S.G.C.A.R.S, Kumhrawand, Jagdalpur, Bastar (C.G.), Indira Gandhi Krishi Vishwavidyalaya, Raipur, ravikiranreddy1819@gmail.com

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Published in „GENETIKA“ Vol. 54, No3(2022), pp. 1023-1034

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203023M
Original scientific article

VITAMINS E AND C EXERT PROTECTIVE ROLES IN HYDROGEN PEROXIDE-INDUCED DNA DAMAGE IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

Misko MILEV¹, Marinela ANGELESKA¹, Milena GEORGIEVA², Viktorija MAKSIMOVA¹,

Milkica JANEVA¹, George MILOSHEV^#², Tatjana RUSKOVSKA^#1

¹Faculty of Medical Sciences, Goce Delcev University, Stip, North Macedonia

²Laboratory of Molecular Genetics, Institute of Molecular Biology, BAS, Sofia, Bulgaria

Abstract

Milev M., M. Angeleska, M. Georgieva, V. Maksimova, M. Janeva, G. Miloshev, T. Ruskovska(2022). Vitamins E and C exert protective roles in hydrogen peroxide-induced DNA damage in human peripheral blood mononuclear cells- Genetika, Vol 54, No.3, 1023-1034.

Hydrogen peroxide (H₂O₂) exerts strong oxidative, cytotoxic, and genotoxic effects, whereas vitamins C and E are potent non-enzymatic antioxidants. This study aimed to demonstrate the ameliorative effects of vitamins C and E, individually or in combination, on H₂O₂-induced DNA damage using the alkaline Comet Assay with silver nitrate staining and visual scoring. Trypan blue exclusion assay was used to determine the cytotoxicity of the treatments, whereas alkaline Comet Assay with silver nitrate staining was used to quantify DNA damage. DNA damage was assessed by the method of visual comet scoring and expressed in arbitrary units. Human peripheral blood mononuclear cells (PBMCs) were pretreated with 100 µM vitamin C and E for 30 min, individually or in combination, followed by a treatment with 100 µM H₂O₂ for 30 min. Untreated cells were used as a negative control, whereas cells treated with 100 µM H₂O₂ only were used as a positive control. We observed a considerable H₂O₂-induced DNA damage in the positive control, which was reduced in vitamin-pretreated cells. The combination of vitamins C and E led to the greatest amelioration of DNA damage. In our hands, Comet Assay with silver nitrate staining and visual scoring represents a rapid and reliable method to investigate the protective effects of vitamins C and E on H₂O₂-induced DNA damage.

Key words: Comet Assay, hydrogen peroxide; vitamin C, vitamin E, silver staining

Corresponding author: Tatjana Ruskovska, Faculty of Medical Sciences, Goce Delcev University, Krste Misirkov 10-a, 2000 Stip, North Macedonia, email: tatjana.ruskovska@ugd.edu.mk

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Published in „GENETIKA“ Vol. 54, No.3 (2022), pp. 1035-1048

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203035P
Original scientific paper

INFLUENCE OF ACE AND ACTN3 GENES POLYMORPHISMS ON CARDIOVASCULAR ADAPTATION IN FEMALE FOOTBALL PLAYERS

Tijana PETROVIC^1*, Marija ZDRAVKOVIC², Marina DJELIC³, Tamara GAVRILOVIC⁴, Zoran MIHAILOVIC¹, Nikola ATANASIJEVIC¹, Oliver STOJKOVIC¹

¹Institute of forensic medicine, Faculty of medicine, University of Belgrade, Belgrade, Serbia

²University Hospital Medical Center “Bezanijska Kosa”, Faculty of medicine, University of Belgrade, Belgrade, Serbia

³Institute of medical physiology, Faculty of medicine, University of Belgrade, Belgrade, Serbia

⁴Serbian Institute of Sports and Sports Medicine, Belgrade, Serbia

Abstract

Petrovic T., M. Zdravkovic, M. Djelic, T. Gavrilovic, Z. Mihailovic, N. Atanasijevic, O. Stojkovic (2022). Influence of ACE and ACTN3 genes polymorphisms on cardiovascular adaptation in female football players. - Genetika, Vol 54, No.3, 1035-1048.

The aim of study was to investigate distribution of ACE and ACTN3 gene polymorphisms in young female footballers and to test association of common gene polymorphisms with body composition, arterial blood pressure and ECG screening variables. A group of 45 white, healthy, adolescent female elite footballers (FG) and 60 sedentary female controls (CG) enrolled in this study. HRM method has been developed to differentiate between variant alleles of ACE and ACTN3 genes. No significant difference was found in the ACE and ACTN3 genotypes or allele frequencies distribution between FG and CG (p>0.05). Also, neither insertion in the ACE gene, nor nonsense mutation in the ACTN3 gene had a significant effect on resting BP and ECG parameters. Cardiovascular adaptation to intensive physical activity in FG is manifested as lowered resting systolic and diastolic blood pressure (lower 18 and 11 percentiles, respectively). Footballers with ACE DD and ACTN3 XX polymorphisms had higher values of Sokolow-Lyon voltage for LV hypertrophy, but without statistically significance (p=0.61 and 0.2, respectively). Interpretation of the effect of specific genes with presumed large effect on sport performance, should be cautious, especially in team sports with a mixed type of physical activity, such as football.

Key words: ACE, ACTN3, elite female football players, gene polymorphisms, cardiovascular adaptation

Corresponding author: Tijana Petrovic, Institute of forensic medicine, Faculty of medicine, University of Belgrade, Dr Subotica 28, Belgrade, Serbia, email: tijana.petrovic@med.bg.ac.rs

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1049-1068

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203049G

Original scientific paper

GENETIC ANALYSIS AND MARKER ASSOCIATION OF PHYSIOLOGICAL TRAITS UNDER RAINFED AND HEAT STRESS CONDITIONS IN SPRING WHEAT (Triticum aestivum L.)

Navin Chander GAHTYARI^1*, Jai Prakash JAISWAL¹, Devender SHARMA^1*, Mohammed TALHA¹, Naveen KUMAR², Narendra Kumar SINGH¹

¹Department of Genetics & Plant Breeding, G.B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand – 263145, India

²Department of Molecular Biology & Genetic Engineering, G.B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand – 263145, India

Abstract

Gahtyari C. N., J. P. Jaiswal, D. Sharma, M. Talha, N. Kumar, N. K. Singh (2022). Genetic analysis and marker association of physiological traits under rainfed and heat stress conditions in spring wheat (Triticum aestivum L.). - Genetika, Vol 54, No.3, 1049-1068.

Identifying gene interactions and markers associated with physiological traits, especially at later stages of grain filling, can help develop effective breeding methodology in wheat crop. Six generations (P₁, P₂, F₁, F₂, BC₁P₁ and BC₁P₂) of four different spring wheat crosses (drought-responsive x drought susceptible) and F₃ generation of a single cross, i.e., MACS6272 x UP2828 were phenotyped and genotyped to decipher gene action and associated markers. Ample variation in canopy temperature depression (CTD – 2.6 – 5.6 °C), chlorophyll content by SPAD (39.6 – 51.3), relative water content (RWC - 51.5 – 75.4 %), grain filling period (GFP - 61.1 – 80.1 days), 100 seed weight (3.7 – 5.5 grams), harvest index (HI - 25.8 – 46.2 %), biological yield (BY – 35.5 – 89.8 grams) and grain yield (GY - 13.4 – 36.5 grams) per plant were observed in six generations. GY positively correlated with CTD, SPAD, 100SW, BY and HI (0.08* - 0.85**). BY had the maximum direct (0.82) and indirect effect via other traits on GY. Significant non-additive epistatic interactions (j & l) and duplicate gene action were found for most traits except GFP and 100SW. Seven different SSR markers associated with CTD, SPAD, NDVI, RWC, 100SW, and explained phenotypic variation (PVE) ranging from 10.1% to 18.4%, with marker Xcfd35 explaining highest PVE for RWC. The identified candidate genes (in silico) belonged to transmembrane proteins (Xcfd32, Xcfd50), nucleic acid binding domains (Xbarc124, Xgwm484) and having enzymatic activity (Xcfd35, Xwmc47, Xwmc728) important for abiotic stress tolerance. Complex inheritance deciphered by six generations indicated delaying the selection to later stages of segregation so that useful transgressive segregants can be selected for improving grain yields in wheat.

Key words: Duplicate gene action, epistasis, single-marker analysis, heat stress, wheat

Corresponding author: Navin Chander Gahtyari; Crop Improvement Division, ICAR - Vivekananda Parvatiya Krishi Anusandhan Sansthan (VPKAS), Almora, Uttarakhand – 263601, India, Mobile: +91-7252956696. E-mail: navin.gahtyari@icar.gov.in; navinnau.pbg@gmail.com

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Published in „GENETIKA“ Vol. 54, No3(2022), pp 1069-1082

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203069S
Original scientific paper

DETECTION OF CARBAPENEM-RESISTANCE AND BIOFILM FORMATION GENES, AND GENETIC RELATEDNESS OF Acinetobacter baumannii ISOLATES

Marko SIMONOVIĆ¹, Zorica LEPŠANOVIĆ¹, Bojan RAKONJAC²_, Srđan LAZIĆ¹

¹Institute of Epidemiology, Military Medical Academy, Belgrade, Serbia

²Institute of Microbiology, Military Medical Academy, Belgrade, Serbia

Abstract

Simonović M., Z. Lepšanović, B. Rakonjac_, S. Lazić (2022). Detection of carbapenem-resistance and biofilm formation genes, and genetic relatedness of Acinetobacter baumannii isolates. - Genetika, Vol 54, No.3, 1069-1082.

Acinetobacter baumannii is one of the most important nosocomial pathogen worldwide. This study aimed to investigate the virulence potential and genomic relatedness of A. baumannii strains isolated from patients hospitalized in the Military Medical Academy (MMA) by detecting OXA-type carbapenemases genes, biofilm-associated genes, and by RAPD analysis. PCR was used to detect the bla_oxagenes, ISAba-1 genetic element, and biofilm-associated genes. The genomic relatedness was determined by RAPD analysis using four different primers (AP2, DAF4. M13, and DECA). bla_oxa-51-like, bla_oxa-23-like, bla_oxa-24-like,and bla_oxa-58-likewere present in 100%, 34.0%, 62.4%, and 3.1% of isolates, respectively. All isolates had the ISAba1 sequence in their genome, in 35.1% of isolates it was associated with the bla_oxa-51-like, and in 97.0% with the bla_oxa-23-like gene. Biofilm-associated genes bap, ompA, epsA, csuA/BABCDE, and pgaABCD were detected in 93.8%, 95.8%, 88.1%, 98.4%, and 98.9% isolates, respectively. RAPD analysis showed a high degree of genome similarity and clonal dispersion of the isolates. Detection of bla_oxa genes, especially biofilm-associated genes, in a high percentage of A. baumannii isolates indicated their great pathogenic potential. RAPD analysis revealed a high level of genomic similarity and clonal dispersion of the majority of isolates through MMA. Further, a continuous introduction of individual strains with different profiles contributes to the genetic diversity of A. baumannii isolates. These results can be useful for further management and tracking nosocomial outbreaks.

Key words: Acinetobacter baumannii; OXA-type carbapenemases; pandrug-resistant; biofilm-forming genes; RAPD

Corresponding author: Marko Simonović, Institute of Epidemiology, Military Medical Academy, 17 Crnotravska St., 11040 Belgrade, Serbia, tel: +381 11 3609 349, E-mail: markosimonovic84@gmail.com

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Published in „GENETIKA“ Vol. 54, No.3 (2022), pp.1083-1100

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2203083A

Original scientific paper

DIVERSITY AND ECOLOGICAL NICHE MODELLING STUDIES IN Trifolium repens L. (WHITE CLOVER) IN THE REGION OF NORTH-WESTERN HIMALAYA, INDIA

Suheel AHMAD¹, Sheeraz Saleem BHAT^1*, Sheikh M. SULTAN², Nazim Hamid MIR¹, Susheel Kumar RAINA², Natarajan SIVARAJ³, Nilamani DIKSHIT⁴, Nazir A. PALA⁵

¹ICAR- Indian Grassland and Fodder Research Institute (IGFRI), Regional Research Station, Srinagar -191132, Jammu & Kashmir, India

²ICAR-National Bureau of Plant Genetic Resources, Regional Station, Srinagar-191132, Jammu & Kashmir, India

³ICAR-National Bureau of Plant Genetic Resources, Regional Station, Hyderabad-500030, Telangana India

⁴ICAR- Indian Grassland and Fodder Research Institute (IGFRI), Jhansi-284003, Uttar Pradesh India

⁵Division of Silviculture & Agroforestry, Faculty of Forestry, SKUAST-Kashmir, India

Abstract

Ahmad S., S. S.Bhat, S. M. Sultan N.H. Mir, S. K. Raina, N. Sivaraj, N. Dikshit^, N. A. Pala (2022). Diversity and ecological niche modelling studies in Trifolium repens L. (white clover) in the region of north-western Himalaya, India. - Genetika, Vol 54, No.3,1083 - 1100.

Trifolium repens L., commonly referred as white clover, is one of the important stoloniferous perennial range legume growing in temperate regions. The introduction of forage legumes in agro-ecosystem provides nitrogen enrichment in soil and mobilizes other nutrients. Further, it has a tremendous potential to help rehabilitate temperate grasslands and decrease the severe fodder shortage in the Himalayan region. In recent decades, collecting and exploration of forage species germplasm, including white clover, have been in the focus of researchers. The collected material shall act as a safe repository for different improvement programmes in future as the germplasm has been stored in the Long Term Module of the National Gene Bank. In this study, maximum entropy (MaxEnt) technique of niche modelling was used to explore probable new areas for the collection of white clover germplasm and identify favorable climate for characterization, cultivation, evaluation and on-farm conservation in the Indian Himalayan region, which comprises the of Jammu Kashmir and Himachal Pradesh and Uttarakhand. Significant variation was observed in plant height (15.5 to 37.6 cm), floret number per flower head (9.24 to 52.4), 100 seed weight (0.038 to 0.077), dry matter yield per plant (6.2 to 15.1 g), leaf length (15.6 to 48.4 mm), leaf width (11.2 to 39.6 mm). Very highly significant variation was also observed in ‘V’ marking. Dendrogram grouped the 22 accessions into two clusters based on the average linking method. Cluster I consisted of five accessions (IC-615818, IC- 615817,IC-622352, IC-615815, and IC-622362), cluster- II could be grouped into sub-cluster–IIA and sub-cluster- IIB. Cluster IIA consist of six accessions (IC- 622338, IC-622379, IC-622382, IC-622401, IC-622343 and IC-62237), whereas cluster-IIB comprise of 11 accessions (IC- 615814 IC- 615811, IC-615819, IC-622376, IC-622383, IC-615812, IC-622385, IC-615816, IC- 615817, IC-622415 and IC-622406). In this study, maximum entropy (MaxEnt) technique of niche modelling was used to explore probable new areas for the collection of white clover germplasm, identifying favorable climate for characterization, cultivation & evaluation and on-farm conservation in the Indian Himalayan region comprising the Union Territory of Jammu Kashmir and the states of Himachal Pradesh and Uttarakhand.

Key words: DIVA-GIS, fodder, germplasm, livestock, MaxEnt Analysis, white clover

Corresponding author: Sheeraz Saleem Bhat. ICAR- Indian Grassland and Fodder Research Institute (IGFRI), Regional Research Station, Srinagar -191132, Jammu & Kashmir, India. E-mail: shrzbhat@gmail.com; Ph. No. +91-7780964030

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1001-1010

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203101S
Original scientific paper

New record for mycobiota of Serbia: A rare fungus Quambalaria cyanescens found in Pelophylax esculentus (Anura) skin microbiome

Miloš Stupar^*, Željko Savković, Katarina Breka, Imre Krizmanić,

Srđan Stamenković, Jelena Vukojević^†, Milica Ljaljević Grbić

University of Belgrade, Faculty of Biology, Belgrade, Serbia

Abstract

Stupar M., Ž. Savković, K. Breka, I. Krizmanić, S. Stamenković, J. Vukojević, M. Ljaljević Grbić (2022). New record for mycobiota of Serbia: a rare fungus Quambalaria cyanescens found in Pelophylax esculentus (Anura) skin microbiome. - Genetika, Vol 54, No.3, 1101-1110.

A rare basidiomycete Quambalaria cyanescens, documented so far on various substrates worldwide, was isolated from the skin of edible frog (Pelophylax esculentus) captured in South Banat. The fungal identification was based on sequencing of ITS region and BLAST analyses. The presence of Q. cyanescens in the amphibian skin microbiome is not only the first finding of this fungus in Serbia but also the recording of new ecological habitat for this rare species of micromycetes. Phylogenetic analyses revealed the high similarity of isolate in this study with foliar pathogens of Eucalyptus in Australia.

Key words: basidiomycete, BLAST, edible frog, ITS, Quambalariaceae

Corresponding author: Miloš Stupar, University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia, E-mail: smilos@bio.bg.ac.rs, tel: +381 11 3244 847, fax: +381 11 3243 603

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Published in „GENETIKA“ Vol. 54, No.3 (2022), pp. 1111-1120

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203111P
Original scientific paper

PCR-RLFP ON IGFBP-3 GENE AND ITS ASSOCIATION ON GROWTH PERFORMANCE OF LAMBS REARED INTENSIVELY

Violeta Caro PETROVIĆ^1*, Dragana RUŽIĆ-MUSLIĆ¹, Nevena MAKSIMOVIĆ¹, Bojana RISTANOVIC², Ivan ĆOSIĆ¹, Dusica OSTOJIĆ-ANDRIĆ¹, Dragan NIKSIĆ¹

¹Institute for Animal Husbandry, Belgrade, Zemun, Serbia

²Faculty of Agriculture - Krusevac, University of Nis, Krusevac, Serbia

Abstract

Petrović Caro Violeta, D. Ružić-Muslić, N. Maksimović, B. Ristanovic, I. Ćosić, D. Ostojić-Andrić, D. Niksić (2022). PCR-RLFP on igfbp-3 gene and its association on growth performance of lambs reared intensively. - Genetika, Vol 54, No.3, 1111-1120.

IGFBP-3 is responsible for the multiple effects of growth factors in most mammalian species and is considered the major transport factor of growth, used as a marker for different body functions such as growth, metabolism, reproduction, body weight control, immunity, energy balance, and so on. Considered as a candidate gene, used as a marker for the growth and production traits as its essential role in the growth and development of the animals. For the DNA extraction, the blood samples are obtained in the jugular vein using a 10 ml vacutainer containing EDTA as a coagulant in the blood collection of each animal Mis breed of sheep (M), Ile de France (F), and Wurttemberg (W). Isolation of DNA performed using the extraction kit (Quick DNA kit) with primers set the Forward and Reverse. The body weights of lambs from birth to 90 days of age, also been calculated. The results of the agarose gel electrophoresis of PCR amplified IGFBP-3 genes for sheep populations Wurttemberg (W), Mis (M), and Ile de France (F) had 654 bp. In our results showed an absence of polymorphism of the IGFBP-3 gene on the tested sheep populations. The results that there is no polymorphism between the examined sheep breeds, in terms of IGFBP-3 genes, we were interested whether there are differences in the body development of lambs of the mentioned populations because IGFBP-3 is related to the growth of animals. All three breeds have similar weights and growth dynamics, which could link to the growth hormone. Analyzing obtained results, we can suggest that absence of a large difference in the growth of the three breeds of sheep W, M, F does not have to be related to the absence of polymorphism of the IGFBP-3 gene but also other genetic and non-genetic factors can affect this trait. To detect the association between genetic polymorphism in IGFBP-3 genes and body development in lambs, DNA sequencing is required, which will be the subject of our future research.

Key words: body weight, insulin like growth factor binding protein -3, lambs, polymorphism, sheep breeds

Corresponding author: Violeta Caro Petrović,Institute for Animal Husbandry, Belgrade - Zemun, Serbia, E-mail: violycaro@yahoo.com; vcaropetrovic@istocar.bg.ac.rs

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1121-1134

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203121G
Original scientific paper

THE NOVEL FII C.*64_*66DEL PROTHROMBIN GENE VARIANT IN WOMEN WITH PREGNANCY LOSS

Maja GVOZDENOV¹, Branko TOMIC¹, Iva PRUNER¹, Mirjana KOVAC²,

Predrag MILJIC³, Darko ANTIC³, Valentina DJORDJEVIC¹

¹Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

²Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Blood Transfusion Institute of Serbia. Hemostasis Department, Belgrade, Serbia

³Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic of Hematology, University Clinical Center, Belgrade, Serbia

Abstract

Gvozdenov M., B. Tomic, I. Pruner, M. Kovac, P. Miljic, D. Antic, V. Djordjevic (2022). The novel fii c.*64_*66del prothrombin gene variant in women with pregnancy loss. - Genetika, Vol 54, No.3, 1121-1134.

Normal pregnancy associated with complex changes of hemostasis, leading to hypercoagulability states. The presence of acquired or genetic prothrombotic risk factors might affect the proper maternal-fetal circulation and result in pregnancy loss. Hence, the screening for the novel prothrombotic variants associated with pregnancy loss would be beneficial. Our aim was to investigate the potential association of recently reported c.*64_*66del variant in prothrombin gene with the etiology of pregnancy loss. Study included 105 women with pregnancy loss and 155 controls. Analyses in patients’ plasma samples, as well as in vitro analyses on transfected Cos-7 cell line were performed in order to investigate the mechanism by which this variant could perturb the coagulation and lead to pregnancy loss. Analyses in patients' DNA and plasma samples involved: DNA sequencing and PCR-RFLP assay for detection of FII c.*64_*66del variant, routine thrombophilia screening, thrombin generation assay and Western blot analysis of prothrombin plasma level. In vitro analyses included transient transfections of Cos-7 cell line with wild-type and c.*64_*66del mutated constructs of pCIneoΔSV40 expression vector. Real-Time PCR and Western blot analysis were used to determine the effect of FII c.*64_*66del variant on mRNA and protein level in constructs. Three women in patients group (2.9%) were detected as heterozygous carriers of FII c.*64_*66del, while none was found among controls. The carriers routine thrombophilia parameters were in reference range and similar prothrombin plasma level in FII c.*64_*66del carriers and non-carriers were detected. The endogenous thrombin potential was slightly increased in FII c.*64_*66del carriers compared to control plasma, but this difference was not statistically significant. Results of in vitro analyses showed significantly decreased prothrombin mRNA and protein level for c.*64_*66del variant compared to wild-type. Results of our pilot study have shown a trend of higher prevalence of FII c.*64_*66del variant in women with pregnancy loss. However, further studies are needed to completely elucidate whether FII c.*64_*66del variant affects prothrombin expression during pregnancy and to account its potential role in etiology of pregnancy loss.

Key words: FII c.*64_*66del, pregnancy loss, 3'untranslated region, prothrombin gene, gene expression.

Corresponding author: Maja Gvozdenov,Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444A, P.O.Box 23, 11010 Belgrade, Serbia, E-mail: maja@imgge.bg.ac.rs, tel: +381 11 3976658, fax: +381 11 3975808

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Published in „GENETIKA“ Vol. 54, No3 (2022), pp1135-1146

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203135N
Original scientific paper

TEMPORAL DIVERSIFICATION IN THE GENUS ONOSMA (BORAGINACEAE) BASED ON NUCLEAR AND PLASTID DNA SEQUENCES

Fatemeh NASROLLAHI^1*, Shahrokh KAZEMPOUR-OSALOO¹, Valyollah MOZAFFARIAN², Hassan ZARE-MAIVAN¹

¹Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

² Department of Botany, Research Institue of Forests and Rangelands, Tehran, Iran

Abstract

Nasrollahi F., S. Kazempour-Osaloo, V. Mozaffarian, H. Zare-Maivan (2022). Temporal diversification in the genus Onosma (Boraginaceae) based on nuclear and plastid Dna sequences. - Genetika, Vol 54, No.3, 1135-1146.

The genus Onosma is a homogeneous taxon with high morphological variation and due to the similarities among the Onosma taxa, there are many problems in their identification. Hence, systematically and taxonomically, it is considered a difficult genus. In the present study, we included a large number of Onosma species throughout their distribution range. Using nuclear (ITS) and two plastids (rpl32-trnL_(UAG) and trnH–psbA) markers, we analyzed the evolutionary history, divergence time and diversification patterns of Onosma across the tropical, subtropical and temperate regions. Divergence time estimates suggest the early radiation of Onosma s.l. happened at the Oligocene-Miocene boundary. BAMM analyses indicate that the best configuration included one significant shift in diversification rates within Onosma: on the branch leading to the clade comprised of species of confined to Iran.

Key words: BAMM, BEAST, Biogeography, Diversification, Phylogenetics

Corresponding author: Fatemeh Nasrollahi, Department of Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Tel.: 00989122538285, E-mail: nbotanist@yahoo.com

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1147-1155

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203147M
Original scientific paper

THE IMPACT OF GAS5 rs145204276 ON DEVELOPMENT AND PROGNOSIS

OF PROSTATE CANCER

Miroslav MIŠOVIĆ^1,2, Predrag ALEKSIĆ^3,2, Miodrag VUKOVIĆ⁴, Dejan KOSTIĆ^1,2,

Nemanja RANČIĆ^5,2, Bojana ALEKSIĆ CIKOTA⁵

¹Institute of Radiology, Military Medical Academy, Belgrade, Serbia

²University of Defense, Medical Faculty-Military Medical Academy, Belgrade, Serbia

³Clinic for Urology, Military Medical Academy, Belgrade, Serbia

⁴Faculty of Biology-University of Belgrade, Belgrade, Serbia

⁵Centre of Clinical Pharmacology, Military Medical Academy, Belgrade, Serbia

Abstract

Mišović M., P. Aleksić, M. Vuković, D. Kostić, N. Rančić, B. Aleksić Cikota (2022). The impact of GAS5 rs145204276 on development and prognosis of prostate cancer. - Genetika, Vol 54, No.3, 1147 - 1155.

The long non-coding RNA (lncRNA) GAS5 can be a marker for early diagnosis and postoperative follow-up in the patients with prostate cancer, whereby lower levels of GAS5 correlate with tumorigenesis and unfavourable clinical course. Expression of the GAS5 can be affected by rs145204276 polymorphism, a 5 base pairs insertion-deletion polymorphism shown as „AGGCA/-“. The aim of this study was to analyse the association between rs145204276 and prostate cancer susceptibility and prognosis.

This study was included 121 healthy subjects and 70 patients with prostate cancer. Diagnosis of prostate cancer was established by histopathology after the surgery. Genotyping was performed by allelic discrimination method using the TaqMan^® assay.

In the healthy subjects, the obtained frequencies of GAS5 rs145204276 genotypes were 80.2% of ins/ins, 16.5% of ins/del and 3.3% of del/del. The allele frequencies were 88.5% of ins and 11.5% of del, respectively. In the patient group, the frequencies of ins/ins, ins/del and del/del genotypes were 70%, 20% and 10%, respectively; the frequency of ins allele was 80% and the frequency of del allele was 20%. Observed frequencies of GAS5 rs145204276 genotypes were not significantly different between healthy subjects and patients with prostate cancer, and also between prognostic groups of prostate cancer.

This study demonstrate no significant association between GAS5 rs145204276 and sussceptibility/prognosis of prostate cancer.

Key words: GAS5, long non-coding RNA (lncRNA), prostate cancer, rs145204276, single nucleotide polymorphism

Corresponding author: Miroslav Mišović, MD,Institute of Radiology, Military Medical Academy,Crnotravska 17, 11000 Belgrade, Serbia,Tel: +381 60 0111177,e-mail: miki.misic@gmail.com

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Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 1157-1169

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203157T

Original scientific paper

USING MOLECULAR MARKERS IN THE IDENTIFICATION OF DIFFERENT GENOTYPES

OF LUCERNE (Medicago sativa L.)

Dragan TERZIĆ¹, Rade STANISAVLJEVIĆ², Tomislav ŽIVANOVIĆ³, Marijenka TABAKOVIĆ⁴,

Nenad TRKULJA², Jordan MARKOVIĆ⁵, Dobrivoj POŠTIĆ²,

Ratibor ŠTRBANOVIĆ^2*

¹ University of Niš, Faculty of Agriculture, Kruševac, Serbia

²Institute for Plant Protection and Environment, Belgrade, Serbia

³University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

⁴Maize Research Institute “Zemun Polje”, Belgrade, Serbia

⁵Institute for Forage Crops Kruševac, Globoder, Serbia

Abstract

Terzić D., R. Stanisavljević, T. Živanović, M. Tabaković, N. Trkulja, J. Marković, D. Poštić, R. Štrbanović (2022). Using molecular markers in the identification of different genotypes of lucerne (Medicago sativa L.). - Genetika, Vol 54, No.3, 1157-1169.

In order to have successful breeding, it is necessary to introduce new breeding material constantly and to use it through various types of hybridisation to increase the existing variability. Ten (10) lucerne varieties of different geographic origin were used in the study. Six varieties originated from the Republic of Serbia (Kruševačka 22, Kruševačka 28, NS-Banat ZMS II, NS-Mediana ZMS V, Zaječarska 83 and Čačanka 10), three varieties originated from the Republic of Croatia (Osječka 66, Osječka 88 and Osječka 99) and one variety originated from the Republika Srpska (Banjalučanka). A total of 100 seeds per each of 10 (ten) lucerne varieties were placed in Petri dishes to germinate. The dishes were placed in the seed germination chamber with the altering temperature of 20 ^oС in the dark for 16 h and 30 ^oС in the light for 8 h for seven days. The first green leaflets of lucerne seedlings (cotyledons) were used for the DNA extraction. The first and the second axes from the principal coordinates analysis accounted for a total of 63.1% of genetic variation, contained in the original dataset. It is clearly observed that the genotype Zaječarska 83 is genetically most distant from other studied lucerne genotypes. These studies confirmed that the observed collection of lucerne varieties is variable enough for the successful breeding process. Using an appropriate breeding model it is possible to breed varieties for certain purposes.

Key words: lucerne, molecular markers, PCoA, RAPD, variety

Correspondin author: Ratibor Štrbanović, Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade, Serbia, Phone: 381 11 2660 049, Fax: 381 11 2669 860, E-mail: ratibor.strbanovic@yahoo.com

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1171-1182

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203171B

Original scientific paper

GENETIC CHARACTERIZATION OF PEPPER (Capsicum annuum L.) GENOTYPES FROM CENTRAL ANATOLIA WITH SSR AND SCAR MARKERS

Hakan BAŞAK¹*, Gölge SARIKAMIŞ², Gamze ÇAKIRER², Mevlüde Alev ATEŞ³

¹Department of Horticulture, Faculty of Agriculture, Kirsehir Ahi Evran University, 40200 Kırşehir, Turkey

² Department of Horticulture, Faculty of Agriculture, Ankara University, 06110 Ankara, Turkey

³Department of Agricultural Biotechnology, Faculty of Agriculture, Kirsehir Ahi Evran University, 40200 Kırşehir, Turkey

Abstract

Başak H., G. Sarikamiş, G. Çakirer, M. A. Ateş (2022). Genetic characterization of pepper (Capsicum annuum L.) genotypes from central Anatolia with SSR and SCAR markers. - Genetika, Vol 54, No.3, 1171-1182.

The major objective in pepper breeding programs is to generate high yielding novel varieties resistant to pests and diseases, tolerant to abiotic stress conditions with improved fruit quality traits including capsaicin content. Germplasm collections are important sources of variability for breeding studies. Molecular markers are important tools to evaluate genetic relationships among germplasm collections. Moreover, markers are used to select the genotypes conferring the desired traits via marker-assisted selection (MAS) as a powerful approach accelerating breeding programs. In the current study, 56 pepper genotypes selected among 313 pepper genotypes collected from Kırşehir province in the

Central Anatolian region of Turkey according to their agronomic and morphological characteristics were used for molecular assays. Six SSR markers two of which were linked to fruit morphology were selected to characterize pepper genotypes according to their high polymorphism information content. Three SCAR markers associated with capsaicinoid synthesis and resistance to Phytophtora capsici (Phyto.5.2) in pepper were used to assess pungency and resistance among genotypes. According to the results obtained with SSR markers, the total number of alleles ranged from 1 to 8 among genotypes. The most polymorphic SSR markers were CaeMS015 and CAMS452 within the pepper population. The genetic distance among genotypes was determined ranging between 0.75-1.00. The segregation of the SCAR marker BF6-BF8 linked to pungency in pepper was determined relative to pungent and sweet reference cultivars. The OP004.717 SCAR marker linked to Phytophtora capsici was tested among genotypes relative to CM334 pepper variety, known as a source of resistance to Phytophtora.

Key words: Capsicum annuum, Capsaicin, Phytophtora capsici, SSR, SCAR

Corresponding author: Hakan Başak, Department of Horticulture, Faculty of Agriculture, Kirsehir Ahi Evran University, 40200 Kırşehir, Turke, E-mail: hbasak@ahievran.edu.tr Phone: +905338147373

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1183-1191

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203183A
Original scientific paper

GENE FLOW AND GENETIC DIVERSITY IN Consolida (Ranunculaceae) USING SEQUENCE RELATED AMPLIFIED POLYMORPHISM

Nakhshin Omer ABDULLA¹, Sahar Hussein HAMARASHID^1*,Syamand Ahmed QADIR², Aryan Mahmood FARAJ³, Sherzad Rasul Abdulla TOBAKARI²

¹ Agricultural project management department / Technical College of Applied Science Halabja /Sulaimani Polytechnic University, Iraq

²Medical laboratory techniques department/Halabja Technical Institute, Research center/Sulaimani Polytechnic University, Sulaymaniyah, Iraq

³Medical Laboratory Science Department/ Technical College Of Applied Science/Sulaimani Polytechnic University, Sulaymaniyah, Iraq

Abstract

Abdulla N. O., S. H. Hamarashid,^, S.A. Qadir, A. M. Fara, S. R. A.Tobakari (2022). Gene flow and genetic diversity in Consolida (Ranunculaceae) using sequence related amplified polymorphism - Genetika, Vol 54, No.3, 1183 - 1191.

The genus Consolida (DC.) Gray (Ranuculaceae) belongs to tribe Delphinieae. It comprises approximately 52 species, including the members of the genus Aconitella Spach. Iraq is one of the richest countries for the genus in South-West Asia.The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Consolida species, were studied through a molecular data. Seventy individuals related to five Consolida were collected in 5 provinces. A total of 75 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of five Consolida species. These bands were produced with the combinations of 5 selective primers. Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Consolida species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iraq.

Key words: Sequence-related amplified polymorphism, Gene Flow; Genetic Diversity, Consolida

Corresponding author: Sahar Hussein Hamarashid, Agricultural Project Management Department/ Technical College of Applied Science/Sulaimani Polytechnic University, Sulaymaniyah, Iraq. E-mail: sahar.rashid@spu.edu.iq

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp.1193-1204

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203193C
Original scientific paper

THE GENETIC DIVERSITY OF SAFFLOWER (Carthamus tinctorius L.) GENOTYPES DEVELOPED BY HYBRIDIZATION USING SSR MARKERS

Emrullah CULPAN* and Burhan ARSLAN

Tekirdağ Namık Kemal University, Faculty of Agriculture, Department of Field Crops, 59030, Süleymanpaşa, Tekirdağ, Turkey

Abstract

Culpan E. and B. Arslan (2022). The genetic diversity of safflower (Carthamus tinctorius L.) genotypes developed by hybridization using SSR markers. - Genetika, Vol 54, No.3, 1193-1204.

Safflower is an annual oilseed crop which has healthy edible oil containing high amount of unsaturated fatty acids in the world. In this study, we investigated safflower registered cultivars of Turkey (4), genotypes retrieved from USDA (10) and their hybrids (45) for genetic variation using 10 simple sequence repeat (SSR) loci. Genetic diversity calculated registered cultivars, genotypes and hybrids were as follows: mean number of alleles (4.67), expected heterozygosity (0.680), average effective number of alleles (3.172), and polymorphism information content (0.664). The dendrogram analysis revealed at least four possible major clusters in the parents and hybrids. High level of genetic diversity explained between the populations and Fst calculate (0.593) suggested that the clusters were differentiated to each other. Registered safflower cultivars of Turkey were distributed across all four clusters and the accessions from USA were defined in most of the clusters. The dendrogram based method analysis revealed two major clusters which corresponded to spiny and spineless safflower genotypes. It was suggested that the studied 6 SSR markers could be utilized for safflower breeding studies based on molecular analysis.

Key words: Carthamus tinctorious L., cultivars, genetic diversity, oilseed, oil content

Corresponding author: Emrullah Culpan, Tekirdağ Namık Kemal University, Faculty of Agriculture, Department of Field Crops, 59030, Süleymanpaşa, Tekirdağ, Turkey, eculpan@nku.edu.tr

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp1205-1216

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203205C
Original scientific paper

SUSCEPTIBILITY LEVELS OF SOME CHESTNUT CULTIVARS AND GENOTYPES TO THE CHESTNUT GALL WASP IN TURKEY

Yusuf ÇİL¹, Ümit SERDAR², Burak AKYÜZ^2,*

¹Ferrero Hazelnut Company, Samsun, Turkey

²Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, Samsun, Turkey

Abstract

Çil Y., Ü. Serdar, B. Akyüz (2022). Susceptibility levels of some chestnut cultivars and genotypes to the chestnut gall wasp in Turkey. - Genetika, Vol 54, No.3, 1205-1216.

The Asian chestnut gall wasp (ACGW) is one of the most important pests threatening most Castanea species. The best management strategy against this pest can be establishing new orchards with resistant cultivars along with biocontrol (parasitoid Torymus sinensis Kamijo (Hymenoptera: Torymidae)). In Turkey, ACGW was first detected in 2014 at Gacık village of Yalova province. Bursa, İstanbul, Sakarya, Kocaeli, Balıkesir, Bilecik, Düzce, Giresun, Bartın, Zonguldak, Sinop and İzmir provinces are also infected with ACGW. This study was carried out to determine the levels of susceptibility of chestnut cultivars/genotypes to ACGW. The study was carried out in Yalova province between 2016 and 2018. In total, 15 cultivars/genotypes were involved in the study, including European (C. sativa) chestnuts (Albayrak, Altınay, ‘Erfelek’, ‘Osmanoğlu’, Salıpazarı, ‘Serdar’, ‘Ünal’), interspecific hybrids (‘Marigoule’ and BDB-L) and complex hybrids (‘Akyüz’, ‘Macit 55’, ‘Ali Nihat’, A9, A55, A56) were tested in the study. In April 2016, five- to seven-year-old seedlings were “bark” grafted, and susceptibility to the ACGW was evaluated by recording the ratio of infected buds (%), visual assessment of the damage (severity), and calculations of an infestation index. Among the cultivars and genotypes tested in the study, the ‘Akyüz’ cultivar was determined to exhibit the highest resistance against the ACGW. To fully understand the resistance mechanism of the ‘Akyüz’ cultivar, molecular and biochemical studies should be done and compared with other results.

Key words: Castanea sativa, complex hybrid, infestation index, Torymus sinensis

Corresponding author: Burak Akyüz, Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, Samsun, Turkey. E-mail: burak.akyuz@omu.edu.tr, +905447232313

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1217-1233

Serbia

UDC 575. 630.11
https://doi.org/10.2298/GENSR2203217G
Original scientific paper

ASSESSMENT OF ELITE WHEAT GERMPLASM FOR RESISTANCE TO FUSARIUM HEAD BLIGHT -A THREAT TO WHEAT PRODUCTION IN NORTH-WEST PAKISTAN

Rubina GUL¹, Muhammad FAHIM²,Sultan Akbar JADOON^3*, Saad Hussain SHAH¹,

Ijaz AHMAD⁴, Masood AHMAD⁵

¹Institute of Biotechnology and Genetic Engineering, University of Agriculture Peshawar-Pakistan 25130

²Center for Omic Sciences, Islamia College Peshawar-Pakistan

³Department of Plant Breeding and Genetics, University of Agriculture Peshawar-Pakistan

⁴Department of Plant Pathology, University of Agriculture Peshawar-Pakistan

⁵Department of Horticulture, University of Agriculture Peshawar-Pakistan

Abstract

Gul R., M. Fahim,S. A. Jadoon, S. H. Shah, I. Ahmad, M. Ahmad (2022). Assessment of elite wheat germplasm for resistance to Fusarium head blight -a threat to wheat production in North-west Pakistan. - Genetika, Vol 54, No.3, 1217-1233.

Fusarium head blight (FHB), caused by Fusarium graminearum, affects both quality and quantity of wheat produce. In Pakistan, due to favorable environmental conditions during spring, FHB can cause significant losses to wheat. Recently, we observed FHB in wheat fields, having 34-84% incidence, along river Swat, Northwest Pakistan. Therefore, elite wheat cultivars and candidate lines in Pakistan as well as exotic-near isogenic lines were screened for FHB resistance using molecular markers, specific for Fhb-1, 2 and 3. Furthermore, all the germplasm was screened for 2NS Translocation - from Triticum ventricosum segment containing cluster of resistance genes for many diseases including FHB. Among Pakistani wheat varieties, Marvi-2000 showed presence of Fhb-2 and 3 specific bands while wheat cv. Saleem-2000 displayed presence of Fhb-1 and 2 specific bands. However, among the candidate lines, L-112, L-105, L-106, L-103 and L-129 exhibited Fhb-1 and 2 specific bands while L-111 alone amplified bands specific to Fhb-1 and Fhb-3. Moreover, 2NS translocation was validated in 2NS near isogenic lines (NILs) obtained from Kansas State University using 2-NS specific marker VENTRIUP and LN2, however, no 2-NS translocation was found in Pakistani varieties as well as candidate lines. In conclusion, none of the Pakistani varieties or candidate lines possessed all sources of FHB resistance in altogether; however, one aliens NIL (Yaccora-Rojo-2NS) surprisingly not only exhibited 2NS translocation but also Fhb-1, 2 and 3 resistant genes.

Key words: disease resistance, food security, Fusarium head blight, mycotoxin, wheat scab

Corresponding author: Sultan Akbar Jadoon,Department of Plant Breeding and Genetics, University of Agriculture Peshawar-Pakistan, sultan@aup.edu.pk

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1235-1248

Serbia

UDC 575. 630.11
https://doi.org/10.2298/GENSR2203235G
Original scientific paper

VARIATION OF HARVEST INDEX OF WHEAT AND TRITICALE IN MONOCROPS AND INTERCROPS SYSTEM OF CULTIVATION

Milosav GRČAK^1*, Dragan GRČAK¹, Radivoje JEVTIĆ², Mirjana LALOŠEVIĆ²,

Vesna ŽUPUNSKI², Branka ORBOVIĆ², Desimir KNEŽEVIĆ¹

¹University of Priština, temporary settled in Kosovska Mitrovica, Faculty of Agriculture, Lešak, Kosovo and Metohia, Serbia

²Institute of Field and Vegetable Crops Novi Sad, National Institute of the Republic of Serbia, Novi Sad, Serbia

Abstract

Grčak, M., D. Grčak, R. Jevtić, M. Lalošević, V. Župunski, B, Orbović, D.Knežević (2022): Variation of harvest index of wheat and triticale in monocrops and intercrops system of cultivation. - Genetika, Vol 54, No.3, 1235-1248.

The harvest index of grain can be used as indicator for the potential yield of crop. The aim of this study was to determine the variation of the harvest index for wheat and triticale and differences in monocrops and intercrops systems of cultivation under different field environmental conditions. The experiment, carried out in field conditions for two consecutive years, was designed according to a randomized block system so that each species was sown in two cultivation systems as a single crop and a combined crop (wheat + pea and triticale + pea) in four replicates. The results show that, in the first year of the experiment, the value of the harvest index varied from 32.5% (triticale + pea) to 39.3% (wheat monocrop), while in the second year of the research, the value of the harvest index varied from 26.4% (wheat + pea) and 28.1% (triticale + pea). As for the components of harvest index, values of weight of grains spike^-1 and weight of total above ground biomass were higher in intercrops than in monocrops system of cultivation. It can be concluded that the intercropping of cereals (triticale and wheat) and forage crops (pea) provided positive effects on weight of grains spike^-1 and weight of total above ground biomass and does not diminish harvest index when compared to standard system of cultivation.

Key words: cultivation system, grain harvest index, pea, small grains

Corresponding author: Milosav Grčak, University of Priština, temporary settled in Kosovska Mitrovica, Faculty of Agriculture, Lešak, Kosovo and Metohia, Serbia, milosavgrcak@gmail.com

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1249-1270

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203249P

Original scientific article

Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer ceLLS

Nina PETROVIĆ^1,2^*, Sercan ERGÜN^3,4, Marija Đorđić CRNOGORAC², Tatjana STANOJKOVIĆ²,

Emina MALIŠIĆ², Ivana Z. MATIĆ²

¹Laboratory for Radiobiology and Molecular Genetics, “VINČA“ Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, Serbia

²Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

³Department of Medical Biology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey

⁴Department of Multidisciplinary Molecular Medicine, Health Sciences Institute, Ondokuz Mayis University, Samsun, Turkey

Abstract

Petrović N., S. Ergün, M. Đorđić Crnogorac, T. Stanojković, .E. Mališić, I. Z. Matić (2022). Hypericum perforatum L. extracts exert cytotoxic effects and show different Mirna signatures in pc-3 and du 145 prostate cancer cells. - Genetika, Vol 54, No.3, 1249-1270.

Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells.The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part.

Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.

Key words: Hypericum perforatum L.; PC-3; DU 145; Cytotoxic activity; MicroRNA

Corresponding author: Nina Petrović, Laboratory for Radiobiology and Molecular Genetics, Department of Health and Environment, “VINČA“ Institute of Nuclear Sciences -National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia, E-mail: dragoninspiration@yahoo.com

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Published in „GENETIKA“ Vol. 54, No3(2022), pp.1271-1284

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203271A
Original scientific paper

EXPLORING THE TEMPO OF SPECIES DIVERSIFICATION IN Astragalus SECTION Incani DC. BASED ON NUCLEAR AND PLASTID DNA SEQUENCES

Elham AMINI¹^*, Shahrokh KAZEMPOUR-OSALOO², Ali Asghar MAASSOUMI³,

Hassan ZARE-MAIVAN²

¹Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

²Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

³Department of Botany, Research Institute of Forests and Rangelands, Tehran, Iran

Abstract

Amini E., Sh. Kazempour-Osaloo, A. A. Maassoumi, H. Zare-Maivan (2022). Exploring the tempo of species diversification in Astragalus section Incani DC. based on nuclear and plastid DNA sequences- Genetika, Vol 54, No.3, 1271 - 1284.

This study uses phylogenetic relationships of the species-rich section Astragalus (Incani), to follow up on recent evidence pointing to rapid and recent plant diversification patterns in the west of Iran. Section Incani is introduced for its taxonomic complication resulting from overlapping morphological characters, but few studies have been done on this section; hence, we also lack a robust time-calibrated chronogram to address hypotheses (e.g., biogeography and diversification rates) that have implicit time assumptions. Two loci (rpl32-trnL_(UAG) and nrDNA ITS) were amplified and sequenced for 87 taxa across Incani for phylogenetic reconstruction and a chronogram in BEAST. Incani is identified as the sister clade to all remaining sections with high support, and within the clade Incani, two strongly supported groups are seen: (1) Clade I includes nine species restricted to eastern Iran and Central Asia, and (2) clade II includes a bulk of the species from west and northwestern Iran, Turkey and southern Europe. Divergence time estimates suggest Incani diverged from remaining sections 3 Mya during the late Pliocene. The crown date for Incani is estimated at 1.5 Mya (Pleistocene). Biogeography showed significant improvement in the likelihood score when the ‘‘jump dispersal” parameter was added. An eastern origin (Central Asia) is implicated as important ancestral area in all deeper nodes. BAMM analyses indicate that the best configuration included one significant shift in diversification rates within Incani: near the crown of Incani (1.5-2 Mya) including clade II. Issues with conducting diversification analyses more generally are examined in the context of scale, taxon sampling, and larger sets of phylogenetic trees.

Key words: BEAST, Biogeography, BAMM, Diversification, Phylogenetics

Corresponding author: Elham Amini, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran, e-mail: Elham.amini@gonbad.ac.ir, Tel.: 00989112712904

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp. 1285-1294

Serbia

UDC 575. 633.15
https://doi.org/10.2298/GENSR2203285D
Original scientific paper

VARIABILITY IN ANTIOXIDANTS IN YELLOW, WHITE, AND RED COLOURED MAIZE GRAIN IN RESPONSE TO DIFFERENT FERTILIZERS

Vesna DRAGIČEVIĆ^1*, Milena SIMIĆ¹, Miodrag TOLIMIR¹, Nenad DJURIĆ², Milena ŠENK¹,

Goran STANKOVIĆ¹, Milan BRANKOV¹

¹Maize Research Institute “Zemun Polje”, Zemun Polje - Belgrade, Serbia

²Megatrend University, Faculty of Biofarming, Bačka Topola, Serbia

Abstract

Dragičević V., M. Simić, M. Tolimir, N. Djurić, M. Šenk, G. Stanković, M. Brankov (2022). Variability in antioxidants in yellow, white, and red coloured maize grain in response to different fertilizers. - Genetika, Vol 54, No.3, 1285-1294.

Maize (Zea mays L.) grain is an important source of nutrients in human diet. The differences in content and relations between certain components of maize grain impact grain colour and its nutritional quality. The objective of the Study was to examine effects of different fertilization systems: mineral fertilizer (urea), organic fertilizer, and bio-fertilizer on white, yellow, and red coloured maize hybrids, regarding grain yield and variations in content of antioxidants: phytate, phenolic compounds, glutathione, carotenoids (yellow pigment), and reduction capacity of DPPH radical. Two-fold higher average grain yield and double fold lower concentration of phenols and carotenoids were present in 2018, in comparison to drier 2017. The lowest phytate content and the highest values of phenols and DPPH reduction capacity were present in red maize kernel, as a hybrid with the highest yield, while in yellow maize kernel, the highest values of yellow pigment and glutathione occurred. The bio-fertilizer expressed the positive impact on reduction of phytate concentration and increase of phenols concentration in maize grain, while urea increased concentration of yellow pigment and glutathione. Correlation analysis showed that reduction in phytate and carotenoids was significant and positive related with grain yield increase, while phenols showed positive correlation with reduction capacity of DPPH radical. Thus, it was shown that changes in fertilization methods could affect antioxidants status in maize grain, particularly in red coloured maize, which besides high yield potential, possess remarkable higher antioxidant capacity in regard to yellow and white coloured maize.

Key words: Antioxidant capacity, Fertilizing, Grain colour, Grain yield

Corresponding author: Vesna Dragičević, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun Polje - Belgrade, Serbia, ^,vdragicevic@mrizp.rs; vdragicevic@yahoo.com; Phone: +381648406163

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Published in „GENETIKA“ Vol. 54, No. 3(2022), pp 1295-1312

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203295A
Original scientific paper

GENETIC DIVERSITY STUDY OF Chrysoperla carnea (Neuroptera: Chrysopidae) POPULATIONS VIA MOLECULAR MARKERS

Fatemeh ABDOLAHADI¹, Alinaghi MIRMOAYEDI^1*, Lila ZARAEI², Samad JAMALI¹

¹Department of Plant Protection, Faculty of Agriculture, Campus of Agriculture and natural Resources, Razi university, Kermanshah, Iran

² Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Campus of Agriculture and natural Resources, Razi university, Kermanshah, Iran

Abstract

Abdolahadi F., A. Mirmoayedi, L. Zaraei, Samad J. (2022). Genetic diversity study of Chrysoperla carnea (Neuroptera: Chrysopidae) populations via molecular markers.- Genetika, Vol 54, No.3, 1295 - 1312.

The objective of this study was to determine the genetic diversity among Chrysoperla carnea samples collected from different locations of Iran (including, East-Azerbaijan, West-Azerbaijan, Isfahan, Kerman, Kermanshah, Lorestan, Mazandaran, Gilan, Hormozgan and Hamedan provinces) using the Inter simple sequence repeat (ISSR) and mitochondrial (Cytochrome Oxidase I – COI) molecular markers in 2016-2018. The results showed that a total of 64 bands were produced by ten primers of ISSR markers which among them 43 bands were polymorphic. The highest and lowest polymorphic percentages belonged to primer UBC-809 (88.88%) and primer UBC-886 (33.33%), respectively. The results of cluster analysis based on ISSR marker data divided the samples into three separate clusters. This grouping was also confirmed by analysis of molecular variance. According to the results of the analysis of molecular variance diversity within and among groups was about 84% and 16%, respectively. In the present study five haplotypes were obtained. The first haplotype (H1) was common in all populations which can be considered as the ancestral haplotype, the other haplotypes have been evolved from it. The novelty of this study is that we report the first time genetic diversity analysis of family Chrysopidae using ISSR and CO1 markers covering more than ten provinces and thirty cities of Iran with a full picture of its genetic diversity. Genetic distance matrix based on Jaccard index indicated low genetic distance of populations. The results showed that ISSR and CO1 markers have high efficiency in study of genetic diversity in the family Chrysopidae.

Key words: Chrysopidae, Genetic diversity, Iran, ISSR, CO1 Marker

Corresponding author: Alinaghi Mirmoayedi, Department of Plant Protection, Faculty of Agriculture, Campus of Agriculture and natural Resources,Razi university, Kermanshah, Iran. E-mail: alimirmoayedi@gmail.com.phone:+98-09181317087

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Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 1313-1330

UDC 575.
https://doi.org/10.2298/GENSR2203313M
Original scientific paper

C9ORF72 REPEAT EXPANSION IS NOT ASSOCIATED WITH ATYPICAL PARKINSONISM

IN THE SERBIAN POPULATION

Ana MARJANOVIĆ^1,2, Valerija DOBRIČIĆ^2,3, Milica JEČMENICA LUKIĆ^1,2,

Iva STANKOVIĆ^1,2, Ognjen MILIĆEVIĆ¹,Nataša DRAGAŠEVIĆ MIŠKOVIĆ^1,2,

Marija BRANKOVIĆ^1,2, Milena JANKOVIĆ ²,Ivana NOVAKOVIĆ¹, Marina SVETEL^1,2,

Elka STEFANOVA^1,2, Vladimir KOSTIĆ ^1,2

¹Faculty of Medicine, University of Belgrade, Belgrade, Serbia,

²Neurology Clinic, University Clinical Center of Serbia, Belgrade, Serbia

³ Lübeck Interdisciplinary Platform for Genome Analytics, University of Lübeck, Lübeck, Germany

Abstract

Marjanović A., V. Dobričić, M. Ječmenica Lukić, I.Stanković, O. Milićević,N. Dragašević Mišković, M. Branković, M. Janković,I. Novaković, M. Svetel, E. Stefanova, V. Kostić (2022). C9ORF72 repeat expansion is not associated with atypical Parkinsonism in the Serbian population. - Genetika, Vol 54, No.3, 1313 - 1330.

Expansion of hexanucleotide repeats (G₄C₂) in the non-coding region of the C9orf72 gene is the most known genetic cause of amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and the combined ALS/FTD phenotype. Besides ALS and FTD, G₄C₂repeat expansions were detected in other neurological disorders with variable frequency. These include, among others, two forms of atypical Parkinsonism, multiple system atrophy (MSA) and progressive supranuclear palsy (PSP). This study aimed to assess the potential role of C9orf72 repeat expansions among Serbian patients diagnosed with MSA and PSP. Genomic DNA of 44 MSA patients, 73 PSP patients, and 96 controls was extracted from peripheral blood, and normal C9orf72 alleles were analyzed by standard quantitative fluorescence polymerase chain reaction (QF-PCR) and fragment analysis. Subsequently, for all samples presenting a single allele, repeat-primed PCR was performed with two different sets of primers to avoid a false-negative result. Thirty repeats were used as a pathogenic cut-off and 20-29 repeats for the intermediate alleles. No pathological C9orf72 expansions were detected in the MSA and PSP patients nor the control subjects. In the MSA group, the most common was the allele with 2 repeats, and the largest repeat number was 14. Among PSP patients, the most common allele also had 2 repeats, while the largest detected repeat size within the normal range was 17. Also, we identified one PSP patient that had an intermediate size allele (25 repeats). We did not find correlation between the number of repeats and disease onset, age at the time of examination, or disease duration in MSA or PSP patients. Regarding family history, in PSP the sum of both allele repeats numbers was higher in patients with positive family history than in sporadic cases. The results presented in this study are the first systematic assessment of C9orf72 allele sizes among patients diagnosed with MSA and PSP in the Serbian population. Although the potential role of intermediate C9orf72 repeats in neurodegenerative disorders is still to be elucidated, our results support the current knowledge that C9orf72 repeat expansions are not associated with MSA and PSP.

Key words: atypical Parkinsonism, C9orf72, multiple system atrophy (MSA), progressive supranuclear palsy (PSP), repeat expansion

Corresponding author: Ana Marjanović, Neurology Clinic, University Clinical Center of Serbia, Doktora Subotica 6, 11000 Belgrade, Serbia, E-mail: ana.marjanovic@yahoo.com, phone:+381113064208

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Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 1331-1349

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203331G
Original scientific paper

ASSOCIATION ANALYSIS OF FOUR HUMAN DOPAMINE PATHWAY GENES WITH ADULT ATTENTION-DEFICIT HYPERACTIVITY DISORDER IN A POPULATION FROM TURKEY

Hilal GÜZEL¹, Halil İbrahim GÜZEL², Cengiz ÇELEBİ³, Mehmet Ali SÖZEN⁴

¹Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Turkey

²Afyonkarahisar State Hospital, Psychiatry Clinic, Turkey

³Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Psychiatry, Turkey

⁴Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Medical Biology, Turkey

Abstract

Güzel H., H. İ. Güzel, C. Çelebi, M. A. Sözen (2022). Association analysis of four human dopamine pathway genes with adult attention-deficit hyperactivity disorder in a population from Turkey. - Genetika, Vol 54, No.3, 1331-1349.

In this tudy, it was aimed to investigate the association/s between dopamine transporter gene (DAT1), dopamine receptor D1 (DRD1), dopamine receptor D2 (DRD2), dopamine receptor D3 (DRD3), dopamine receptor D4 (DRD4) gene variants and adult Attention Deficit and Hyperactivity Disorder (ADHD). A prospective analytical case control study. A total of 128 ADHD cases and 100 non-ADHD controls from Western population of Turkey were included in this study. DNA was isolated from peripheral blood. Genotype and allele frequency P-values were calculated by Chi square (c2) and Fisher Exact tests. Other statistical analyses were carried out using SPSS program version 20.0. The genotypes for the DAT1, DRD2, DRD3 and DRD4 variants were identified by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP). 4R allele and 4R/4R genotype of Exon 3 VNTR polymorphism in the DRD4 gene were observed to be the most frequent one in both case and control groups. 4R allele was found to be statistically significant in ADHD group than the ones in control group (p=0.01). No statistical differences in the genotype and allele frequencies were observed between ADHD cases versus non-ADHD controls for DAT1, DRD2 and DRD3 polymorphisms.

A statistically significant association was found only between DRD4 Exon 3 VNTR polymorphism and adult ADHD. However, to confirm that these gene variants contributes to ADHD and ADHD-subtypes, further studies with both higher population sizes and many candidate genes are needed to be investigated simultaneously.

Key words: Adult Attention Deficit Hyperactivity Disorder (ADHD), Association analysis, Dopamine Pathway Genes, Polymorphism

Corresponding author: Mehmet Ali Sozen, Zafer Sağlık Külliyesi A Blok, Dörtyol Mah. 2078 Sok. No:3 Afyonkarahisar/Turkey, E-mail: masozen@hotmail.com, Tel: +90 272 246 3301 Fax: +90 272 246 3300

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Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 1351-1364

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203351B
Original scientific paper

ANALYSIS OF “CLINICAL EXOME” PANEL IN SERBIAN PATIENTS WITH COGNITIVE DISORDERS

Marija BRANKOVIĆ^1,2, Elka STEFANOVA^1,2, Gorana MANDIĆ^1,2, Ana MARJANOVIĆ^1,2, Valerija DOBRIČIĆ³, Aleš MAVER⁴, Gaber BERGANT⁴, Zorica STEVIĆ¹, Milena JANKOVIĆ¹, Ivana NOVAKOVIĆ², Borut PETERLIN⁴, Vladimir KOSTIĆ^1,2

¹Neurology Clinic, University Clinical Center of Serbia, Belgrade, Serbia

²Faculty of Medicine, University of Belgrade, Belgrade, Serbia

³Luebeck Interdisciplinary Platform for Genome Analytics (LIGA), University of Luebeck,

Luebeck, Germany

⁴Clinical Institute of Medical Genetics, University Medical Centre Ljubljana, Ljubljana, Slovenia

Abstract

Branković M., E. Stefanova, G. Mandić, A. Marjanović, V. Dobričić, A.Maver, G. Bergant, Z. Stević, M. Janković, I. Novaković, B. Peterlin, V. Kostić (2022). Analysis of “clinical exome” panel in Serbian patients with cognitive disorders. - Genetika, Vol 54, No.3, 1351-1364.

As life span rises, dementia has become a growing public health issue. According to current estimates, almost 50 million people worldwide have dementia, and the number is expected to grow. Next generation sequencing (NGS) methods have helped significantly with identifying causative gene variants related to various cognitive disorders. Our study aimed to analyze the genetic basis of cognitive disorders using NGS clinical exome panel. The study included a total number of 15 unrelated cases diagnosed with cognitive disorders, all negative after standard targeted genetic testing was performed (available at Neurology Clinic, UCCS, Belgrade, Serbia). Preference was given to familial cases with early presentation or complex phenotype. Sequencing of a clinical exome (CE) panel for 4813 genes with known associated clinical phenotypes was performed using TruSight One sequencing panel on an Illumina MiSeq NGS platform according to the manufacturer’s instructions (Illumina, San Diego, CA, USA). Variants were analyzed with Illumina Variant Studio v3 software provided by Illumina as well as a previously developed pipeline. Variants analysis and interpretation were based on phenotype gene target approach, literature and databases search, allele frequency, and pathogenicity prediction by in silico software. All causative variants were confirmed by Sanger sequencing. Whenever possible, additional family members were studied for segregation analysis. CE panel analysis revealed a likely genetic cause in four patients. We have detected two missense heterozygous pathogenic variants in the PSEN1 gene in one patient each and homozygous nonsense pathogenic variant in the OPTN gene in two more patients. Detected pathogenic variants are in line with the clinical phenotype of our patients. In the rest of the 11 cases, genetic diagnosis remains unclear. The results of our study emphasize the significance of CE panel analysis in establishing a diagnosis for patients with dementia. Furthermore, give us insight into the complexity of the genetic background of this group of disorders.

Key words: cognitive impairments, DNA diagnostics, gene panels, gene variant, PSEN1, OPTN

Corresponding author: Marija Branković, Neurology Clinic, University Clinical Center of Serbia, dr Subotića starijeg 6, 11000 Belgrade, Serbia, Phone:+381112658355,e-mail: mara.brankovic@gmail.com

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Published in „GENETIKA“ Vol. 54 No.3(2022), pp. 1365-1374

Serbia

UDC 575. 633.15
https://doi.org/10.2298/GENSR2203365K
Original scientific paper

Effect of reciprocal crosses on grain yield and other agronomic traits in maize

Aleksandar KOVAČEVIĆ^1*, Jovan PAVLOV¹, Milan STEVANOVIĆ¹, Nikola GRČIĆ¹,

Marko MLADENOVIĆ¹, Nenad DELIĆ¹, Nemanja KNEŽEVIĆ²

¹Maize Research Institute “Zemun Polje”, Zemun Polje, Serbia

² Faculty of Agriculture University of Belgrade, Serbia

Abstract

Kovačević A., J. Pavlov, M. Stevanović, N. Grčić, M. Mladenović, N. Delić, Ne. Knežević (2022). Effect of reciprocal crosses on grain yield and other agronomic traits in maize. - Genetika, Vol 54, No.3, 1365-1374.

The goal of this experiment was to examine a possible influence of reciprocal crosses on grain yield and some morphological traits in maize. Field trials were set up on three locations (Zemun Polje, Pančevo, Bečej) during 2015 and 2017. Five elite inbred lines were selected in order to produce hybrid combinations for the trial, two of them were of Lancaster origin and used as tester lines (ZPT1, ZPT2), while remaining three had a Non Lancaster origin (ZPL1, ZPL2 and ZPL3). By performing reciprocal crossings between Lancaster and non Lancaster inbred lines, twelve single cross hybrids were produced for the trial. Trials were set up on three locations during two years and grain yield, grain moisture, plant height, ear height and mass of 1000 kernels were analyzed. Location, year and reciprocal crosses were significant factors for all examined traits. Reciprocal crosses had statistical significance on two hybrid combinations in terms of grain yield and grain moisture. SNPs molecular markers were used to assess the genetic diversity of the inbred lines involved in this experiment. When it comes to plant height and mass of thousand kernels, a statistically significant difference was observed in one hybrid combination. The largest reciprocal differences between original and reciprocal hybrids were recorded in grain yield, which were statistically significant for the crosses ZPH4/ZPH4R and ZPH2/ZPH2R (20.03% and 19.49% respectively). The ZPH2/ZPH2R hybrid combination is the combination with the statistically significant differences between the original and reciprocal hybrid, for all evaluated traits except for grain moisture. Reciprocal hybrids ZPH5/ZPH5R and ZPH6/ZPH6R with ZPL3 as their maternal inbred line didn’t express a statistically significant differences between both variants, e.g. their reciprocal effect was low.

Key words: grain yield, inbred lines, maize, morphological traits, reciprocal effect

Corresponding author: Aleksandar Kovacevic, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1,11185 Zemun Polje, Serbia, email: email: akovacevic@mrizp.rs

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Published in „GENETIKA“ Vol. 54, No.3(2022), pp. 1375-1384

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203375V
Original scientific paper

PPARGC1A GENE POLYMORPHISM AND ITS ASSOCIATION WITH OBESITY-RELATED METABOLIC TRAITS IN SERBIAN ADOLESCENT POPULATION

Vanja VIDOVIĆ^1*, Nela MAKSIMOVIĆ², Stojko VIDOVIĆ¹, Tatjana DAMNJANOVIĆ²,

Irina MILOVAC¹, Ivana NOVAKOVIĆ²

¹Faculty of Medicine, Department of Human Genetics, University of Banja Luka, the Republic of Srpska, Bosnia and Herzegovina

²Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Serbia

Abstract

Vidović V., N. Maksimović, S. Vidović, T. Damnjanović, I. Milovac, I. Novaković (2022). PPARGC1A gene polymorphism and its association with obesity-related metabolic traits in Serbian adolescent population. - Genetika, Vol 54, No.3,1375 - 1384.

PPARGC1A is involved in many metabolic processes including normal mitochondrial biogenesis, oxidation of glucose and lipids and transport of glucose into skeletal muscles. Previous researches linked this polymorphism with the higher risk of developing type 2 diabetes, metabolic syndrome and obesity. The aim of the study was to investigate the association of Gly482Ser with body mass index (BMI), fasting glucose levels and lipid profile in Serbian adolescents. The study included 147 boys and 150 girls, 15 years of age. Anthropometric and biochemical parameters were recorded. Cardiovascular and malignant diseases, type 2 diabetes, cerebral palsy and genetics syndrome were criteria for exclusion. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (RFLP) assay. The results showed that boys carriers of GG genotype had statistically higher mean values of TC compared to the boys who were carriers of GA+AA genotypes (p=0.033). However, statistical significance was not obtained for the other analyzed parameters. Furthermore, in the group of overweight and obese children, higher mean values of TC and LDL-C were observed in the carriers of GG genotype compared to carriers of GA+AA genotype for all the adolescents, as well as in the group of girls. No correlation was observed for values of BMI, fasting blood glucose and levels of triglycerides. To confirm these results, further research with larger sample size and non-genetics factor taking into consideration, would be of great interest.

Key words: Body mass index, glycaemia, lipid parameters, Gly482Ser polymorphism

Corresponding author: Vanja Vidović, Faculty of Medicine, Department of Human Genetics, University of Banja Luka, the Republic of Srpska, Bosnia and Herzegovina,Tel: 0038765888475; Fax: 0038751234100; E-mail: vanja.vidovic@med.unibl.org

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Published in „GENETIKA“Vol. 54, No.3(2022), pp. 1385-1397

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203385K
Original scientific paper

Ecological-genetic model in grass pea (Lathyrus sativus L.) breeding

Valentin Kosev¹, Viliana Vasileva¹, Vera POPOVIC²^*, Vladan PEŠIĆ³, Miloš NOŽINIĆ⁴

¹Institute of Forage Crops, Pleven, Agricultural Academy, Bulgaria.

² Institute of Field and Vegetable Crops, Novi Sad, Serbia

³University of Belgrade, Faculty of Agriculture, Zemun - Belgrade, Serbia

⁴PI Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia &Herzegovina

Abstract

Kosev V., V. Vasileva V., Popovic, V. Pešić, M. Nožinić (2022). Ecological-genetic model in grass pea (Lathyrus sativus L.) breeding. - Genetika, Vol 54, No.3, 1385-1397.

ecological model for organizing the quantitative traits and the method of orthogonal regressions were applied to evaluate both, aboveground and root biomass of grass pea varieties different originating. The study was conducted for three years. The highest yields for fresh aboveground biomass were BGE015741 (840.40 kg/da), LAT4362 (779.3 kg da^-1) and BGE027129 (722.80 kg da^-1). Plants of LAT4362 and BGE025277 have a higher weight of fresh aboveground mass and fresh root mass and exhibit a good combination of adaptive and attraction genes. The highest average seed yield was recorded at BGE015741 (158.40kg da^-1), BGE027129 (113.10 kg da^-1) and BGE025277 (108.30kg da^-1). The BGE027129, BGE025277 and BGE015741 varieties are found of greatest interest with regard to seed weight per plant and they are suitable as initial materials for the purpose of combinatorial breeding for the obtaining of genotypes combining both, high seed weight and high root biomass weight per plant.

Key words: genotype,module, phenotype, resultant traits

Corresponding author: Viliana Vasileva, Institute of Forage Crops, Pleven, Agricultural Academy, Bulgaria viliana.vasileva@gmail.com; vera.popovic@ifvcns.ns.ac.rs

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Published in „GENETIKA“Vol. 54, No3(2022), pp. 1399-1410

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2203399K

Original scientific paper

THE IMPACT OF HEREDITARY THROMBOPHILIAS IN RECURRENT PREGNANCY LOSS

Ege Riza KARAGUR¹, Mustafa Tarik ALAY², Aydin DEMİRAY¹, Nedim KARAGENC¹,

Onur TOKGÜN¹, Taner DURAK¹, Hakan AKCA^1*

¹Departmnet of Medical Genetic, School of Medicine Pamukkale University, Pamukkale, 20160 Denizli, Turkey

² Department of Medical Genetics, Cerrahpaşa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey

Abstract

Karagur R.E., M. T. Alay, A. Demiray, N.Karagenc, O. Tokgün, T. Durak, H. Akca (2022). The impact of hereditary thrombophilias in recurrent pregnancy loss. - Genetika, Vol 54, No.3, 1399-1410.

Introduction: Recurrent pregnancy loss (RPL) is defined as two or more consecutive pregnancy loss which occurs before the 20^th weeks of pregnancies for the last menstrual period. Hereditary cause of thrombophilic gene mutations and polymorphism may play an essential role in RPLs.

Material and Method: 291 women with a history of two or more consecutive abortions as a study group and 61 women without the history of miscarriages as a control group were included in a study. In this study we analysed the effects of Factor II Prothrombin mutation, FV Leiden mutation, MTHFR C677T, MTHFT A1298C, PAI-1, β-fibrinogen, Factor XIIIA (V34L) and Glycoprotein IIIa (L33P) polymorphisms on RPL by using pyrosequencing. Chi-square and multiple regression analysis were used for statistical analysis.

Results: FII prothrombin mutation, FV Leiden mutation, MTHFR C677T, MTHFR A1298C, PAI1 and Beta fibrinogen were found statistically significant in the chi-square test. Heterozygous FV G1691A (OR:8.092, CI: 1.280-51.165), homozygous MTHFR A1298C (OR:17.621, CI: 3.644 - 85.203), Heterozygous MTHFR C677T (OR: 2.921 CI: 0.811-10.515), Homozygous MTHFR C677T (OR: 3.619 CI: 1.647-7.954), heterozygous MTHFR A1298C (OR: 5.989, CI: 2.574-13.934), homozygous PAI1 (OR: 8.756, CI: 2.805 -27.334), heterozygous PAI1 ( OR: 7.114, CI: 3.145- 16.096) homozygous FibrinogenG455A (4.085, CI: 1.438-11.610) were found statistically significant in logistic regression analysis for RPL(p<0.05).

Discussion: This study indicated that there is a significant association between thrombophilias and RPL. Therefore, it is important to detect thrombophilic mutations in RPL.

Key words: Hereditary, thrombophilia, pregnancy loss, pyrosequence

Corresponding author: Prof. Dr. Hakan Akca, Pamukkale University School of Medicine Department of Medical Genetic Pamukkale/Denizli-Turkey, e-mail: hakca@pau.edu.tr, Phone: +90(258) 296 16 68

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Published in „GENETIKA“Vol. 54, No.3(2022), pp 1411-1428

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203411K

Original scientific paper

VARIABILITY OF SOME TRAITS OF WALNUTS (Juglans regia L.)

IN THE TEST OF HALF SIB, ORIGINATING IN THE ĐERDAP FOREST

Milica KOVAČ^1*, Branislav KOVAČEVIĆ², Saša ORLOVIĆ^1,2

¹ Faculty of Agriculture, University of Novi Sad, Serbia

² Institute of lowland forestry and environment, University of Novi Sad, Serbia

Abstract

Kovač M., B. Kovačević, S. Orlović (2022). Variability of some traits of walnuts (Juglans regia L.) in the test of half sib, originating in the Đerdap forest- Genetika, Vol 54, No.3, 1411-1428.

As part of efforts for protection and restoration of variability of Juglans regia in Đerdap gorge, Serbia, where it grows as autochthonous relict species, the variability of ten measured and nine derived leaf morphometric parameters of half-sib progenies Persian walnut originating from Đerdap gorge was studied in this work. According to the contribution to total expected variance, parameters that were most effected by differences between half-sib progenies are leaf width (LW), side leaflet length (lL) and top leaflet length (20-30%). Using loadings with the first four rotated principal components, describing 90.1% of total variance, all parameters were grouped in four groups, where parameters LW, lL and lt were in the first group, suggesting multicollinearity between them. These parameters had also relatively high loadings with the first canonical variable from canonical discriminant analysis. Only one of them (lt) was selected by forward stepwise discriminant analysis, where model with four selected leaf parameters achieved 26.5% of correct allocation, while model with all studied parameters achieved 62.4% of correct allocation. Half-sib progenies agglomerated in three clusters, where two small clusters originate from trees that were close to sheltered valleys of small tributaries to river Danube, opposite to mother trees of the first cluster progenies, that were exposed to dominant winds.

Key words: Genetic diversification, gene transfer; Hedera, Sequence-related enhanced polymorphism Persian walnut, conservation, multivariate analysis, variability

Corresponding author: Milica Kovač, University of Novi Sad,Faculty of Agriculture,Dositeja Obradovića Sq. 8,21000 Novi Sad, Serbia, E-mail: mici_kovac@hotmail.rs, milica.kovac@polj.uns.ac.rs

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Published in „GENETIKA“Vol. 54, No.3(2022), pp.1429-1445

Serbia

UDC 575. 630
https://doi.org/10.2298/GENSR2203429Y

USE OF LINEAR MODELING, MULTIVARIATE ADAPTIVE REGRESSION SPLINES AND DECISION TREES IN BODY WEIGHT PREDICTION IN GOATS

Abdulmojeed YAKUBU¹*, Ecevit EYDURAN², Senol CELIK³, Juliana O. ISHAYA¹

¹Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, P.M.B. 135, Lafia, 950101, Nigeria.

²Departments of Animal Science and Business Administration, Igdir University, Igdir, Turkey

³Department of Animal Science, Faculty of Agriculture, Bingol University, Bingol, Turkey

Abstract

Yakubu A., E. Eyduran, S. Celik, J. O. Ishaya (2022). Use of linear modeling, multivariate adaptive regression splines and decision trees in body weight prediction in goats. - Genetika, Vol 54, No.3, 1429-1445.

Use of robust regression algorithms for better prediction of body weight (BW) is receiving increased attention. The present study therefore aimed at predicting BW from chest circumference, breed and sex of a total of 1,012 goats. The animals comprised 332 matured West African Dwarf (WAD) (197 bucks and 135 does), 374 Red Sokoto (RS) (216 bucks and 158 does) and 306 Sahel (SH) (172 bucks and 134 does) randomly selected in Nasarawa State, north central Nigeria. BW prediction was made using automatic linear modeling (ALM), multivariate adaptive regression splines (MARS), classification and regression tree (CART), chi-square automatic interaction detection (CHAID) and exhaustive CHAID. The predictive ability of each statistical approach was measured using goodness of fit criteria i.e. Pearson’s correlation coefficient (r), Coefficient of determination (R²), Adjusted coefficient of determination (Adj. R²), Root-mean-square error (RMSE), Mean absolute percentage error (MAPE), Mean absolute deviation (MAD), Global relative approximation error (RAE), Standard deviation ratio (SD _ratio), Akaike’s information criterion (AIC) and Akaike’s information criterion corrected (AICc). Male RS and SH goats had significantly (P<0.05) higher BW and CC compared to their female counterparts while in WAD, male goats had significantly (P<0.05) higher CC (57.88±0.51vs. 55.45±0.55). CC was determined to be the trait of paramount importance in BW prediction, as expected. Among the five models, MARS algorithm gave the best fit in BW prediction with r, R², Adj. R², SD_ratio, RMSE, RAE, MAPE, MAD, AIC and AICc values of 0.966, 0.933, 0.932, 0.26, 1.078, 0.045, 3.245, 0.743, 186.0 and 187.0, respectively. The present information may guide the choice of model which may be exploited in the selection and genetic improvement of animals including feed and health management and marketing purposes, and especially in the identification of the studied breed’s standards.

Key words: body weight, goats, modelling, regression algorithms, tropics

Corresponding author: Abdulmojeed Yakubu,Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, P.M.B. 135, Lafia, 950101, Nigeria, email address: abdulmojyak@gmail.com; abdulkubu@nsuk.edu.ng

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Published in „GENETIKA“Vol. 54, No.3(2022), pp.1447-1463

Serbia

UDC 575. 633.11
https://doi.org/10.2298/GENSR2203447R

GENOTYPE BY ENVIRONMENT INTERACTION ANALYSIS FOR GRAIN YIELD

OF WHEAT IN IRRIGATED AND RAIN-FED MEGA-ENVIRONMENTS USING AMMI AND GGE BIPOLT MODELS

Mohammad Hossein ROMENA, Abdollah NAJAPHY*, Mohsen SAEIDI, Mahmud KHORAMIVAFA

Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran

Abstract

Romena H. M., A. Najaphy, M. Saeidi, M. Khoramivafa (2022). Genotype by environment interaction analysis for grain yield of wheat in irrigated and rain-fed mega-environments using AMMI and GGE bipolt models. - Genetika, Vol 54, No.3, 1447-1463.

Wheat (Triticum aestivum L.) is the major and strategic cereal crop globally. It is grown worldwide under a wide range of agro-ecological conditions. The performance of quantitative traits, for example grain yield, often varies due to significant effects of the genotype and environment interaction (GEI). Therefore, the integration of higher grain yield with stable performance is one of the common objectives in wheat-breeding programs. The present investigation was carried out to evaluate the GEI through GGE biplot and AMMI analysis over six environments (rain-fed and irrigated conditions during three years) using 29 diverse wheat genotypes. The analysis of variance revealed that the effect of environments (E), genotypes (G) and GEI are significant. The first two AMMI components justified 72.6% of the GEI variation. In the other hands, the first two principal components of the GGE biplot explained 58.3% of the observed variation for the grain yield. The GGE biplot suggested suitability of the tester E6 based on discrimination ability and representativeness, which is ideal for selecting superior genotypes. Based on the similar results of AMMI and GGE-biplot methods, the genotype G6 was the best performing genotypes at the rain-fed mega-environment. In addition, the entries G1 and G29 were suitable for the irrigated mega-environment.

Key words: Adaptability analysis, Multi-environment trials, Stability analysis, Modified AMMI stability values (MASVs), Triticum aestivum

Corresponding author: Abdollah Najaphy, Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran, Email: anajaphy@razi.ac.ir; nadjaphy@yahoo.com

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