GENETIKA, Vol. 55, No.1 (2023)
Ana MARJANOVIC, Aleksa PALIBRK, Valerija DOBRICIC, Ognjen MILICEVIC, Marija BRANKOVIC, Vanja VIRIC, Aleksandra DRINIC, Gorana MANDIC STOJMENOVIC, Milena JANKOVIC, Ivana BASTA, Stojan PERIC, Ivana NOVAKOVIC, Elka STEFANOVA, Zorica STEVIC
C9orf72 GENETIC SCREENING IN AMYOTROPHIC LATERAL SCLEROSIS PATIENTS FROM SERBIA [Abstract] [Full text]
Mohammad Reza ESLAMZADEH-HESARI, Mansoor OMIDI, Varahram RASHIDI, Alireza ETMINAN, Alireza AHMADZADEH
INVESTIGATION OF MOLECULAR VARIABILITY IN SOME Aegilops SPECIES USING START CODON TARGETED POLYMORPHISM (SCoT) AND CAAT-BOX DERIVED POLYMORPHISM (CBDP) MARKERS [Abstract] [Full text]
Manisha THAKUR, Ramesh KUMAR, Parul SHARMA, Rajnish SHARMA
ASSESSMENT OF GENETIC PURITY AND EARLINESS
IN F1 AND F2 POPULATION OF CUCUMBER (Cucumis sativus
L.) USING SSR MARKERS [Abstract]
[Full
text] [Supplement]
Sudheer Kumar ANNEPU, Happy Dev SHARMA, Anupam BARH, Rajesh DOGRA, Vipin SHARMA, Shivender THAKUR, Vinay VERMA, Kanika SHARMA
PERFORMANCE PREDICTION OF F1 CROSSES IN EGGPLANT (Solanum melongena L.) BASED ON MORPHOLOGICAL AND MOLECULAR DIVERGENCE [Abstract] [Full text] [Supplement]
Serife BALCI, Volkan Mehmet CINAR, Aydin UNAY
ESTIMATING GENE ACTION AND COMBINING ABILITY FOR YIELD AND FIBER QUALITY IN COTTON (Gossypium hirsutum L.) [Abstract] [Full text]
Morteza GHANADPOUR, Seyed Reza KAZEMI NEZHAD, Hamid GALEHDARI, Mohammadreza HAJJARI
HOTAIR/MIR1 AXIS ACTS AS A POTENTIAL CHEMOTHERAPY
TARGET IN GASTRIC CANCER [Abstract] [Full
text]
Ege Riza KARAGUR, Aydin DEMIRAY, Nedim KARAGENC, Esra ELVER, Onur TOKGUN, Arzu YAREN, Gamze G. DOGU, Hakan AKCA
IS THERE AN ADVANTAGE OF MONITORING VIA EXOSOME-BASED DETECTION OF EGFR MUTATIONS DURING TREATMENT IN NON-SMALL CELL LUNG CANCER PATIENTS? [Abstract] [Full text]
Koray OZRENK, Akgul TAS, Muttalip GUNDOGDU, Nurhan KESKIN, Sezai ERCISLI
PHYSICOCHEMICAL SUBSTANCES AND BIOACTIVE COMPONENTS OF WILD CORNELIAN CHERRY (Cornus mas L.) FRUITS IN ERZİNCAN PROVINCE OF EASTERN TURKEY [Abstract] [Full text]
Menisha RANI, Salesh Kumar JINDAL, Om Prakash MEENA
GENETIC VARIABILITY FOR PLANT GROWTH, FRUIT, SEED AND
BIOCHEMICAL TRAITS IN BELL PEPPER BREEDING POPULATIONS UNDER NORTH INDIAN
PLAINS [Abstract] [Full
text]
AMMI ANALYSIS FOR GRAIN YIELD IN BREAD WHEAT RECOMBINANT INBRED LINES [Abstract] [Full text]
Lijuan WU, Zaijun YANG, Junqiang WANG3, Jundong HE3, Jin JIANG, Fang FANG, Shian SHEN3, Xinkun HU
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS
OF NOVEL Wx GENES FROM Psathyrostachys juncea AND Eremopyrum bonaepartis [Abstract] [Full text]
Renata ILIČIĆ, Aleksandra JELUŠIĆ, Milan BLAGOJEVIĆ, Ferenc BAGI, Karolina VRANDEČIĆ, Slaviša STANKOVIĆ, Tatjana POPOVIĆ MILOVANOVIĆ
CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA [Abstract] [Full text]
Rukiye KARA and Aydın AKKAYA
GENOTYPIC DIFFERENCES FOR CHLOROPHYLL, ASH AND N CONTENTS AND THEIR RELATIONS WITH GRAIN YIELD IN TURKISH BREAD WHEAT LANDRACES [Abstract] [Full text]
Svetlana M. PAUNOVIĆ, Rade K. MILETIĆ
FRUIT CHARACTERISTICS OF PROMISING WALNUT GENOTYPES FROM THE REGION OF EASTERN SERBIA [Abstract] [Full text]
M. Muchlish ADIE, Arifin ARIFIN, Suyamto SUYAMTO, Ayda KRISNAWATI
ESTIMATION OF HETEROSIS, HETEROBELTIOSIS, AND GENE ACTION IN F1 PLANTS FROM SMALL-SEEDED SOYBEAN CROSSES [Abstract] [Full text]
Emre SEVINDIK, Makbule BOZKURT, Mehmet YILMAZ, Ezginur ŞENYÜZ, Mehmet Yavuz PAKSOY
MOLECULAR CHARACTERIZATION OF Dittrichia viscosa
(L.) GREUTER (ASTERACEAE) POPULATIONS
REVEALED BY ISSR MARKERS AND CHLOROPLAST (cpDNA) trnL INTRON SEQUENCES [Abstract] [Full
text]
Masoud SHEIDAI, Sogol ALLAHVERDI, Narges ANVARI, Somayeh EIVAZI KHAMENE, Mozhgan GHANDEHARI ALAVIJEH, Marziyeh SEYED GHOREISHI, Fatemeh JAMSHIDI2, Ghazal KHOSRAVAN, Naser KALHOR, Fahimeh KOOHDAR
TEXT-11 SNPS AND NEIGHBORING SEQUENCES ROLE IN CAUSING MALE INFERTILITY IN SOME ETHNIC GROUPS OF IRANIAN AND THEIR POTENTIAL ROLE IN ESTIMATING DIVERGENCE TIME [Abstract] [Full text]
Maja MATOŠA KOČAR, Aleksandra SUDARIĆ, Tomislav DUVNJAK, Zoe ANDRIJANIĆ, Antonela MARKULJ KULUNDŽIĆ
PROGRESS, ADAPTABILITY AND STABILITY OF SOYBEAN GRAIN YIELD AND GRAIN QUALITY IN CONVENTIONALLY CREATED ELITE LINES [Abstract] [Full text]
Zorana DOBRIJEVIĆ, Jelena KARANOVIĆ, Dušanka SAVIĆ-PAVIĆEVIĆ, Goran BRAJUŠKOVIĆ
THE EFFECT OF EPISTATIC INTERACTIONS BETWEEN GENETIC VARIANTS LOCATED IN MICRORNA AND SILENCING COMPLEX GENES ON PROSTATE CANCER PROGRESSION RISK [Abstract] [Full text]
Guzide DOGAN, Seda ORENAY BOYACIOGLU, Metin CALISKAN, Elmas KASAP, Semin AYHAN, Erhun KASIRGA
ASSOCIATION OF MICRO RNA EXPRESSIONS WITH PEDIATRIC CELIAC CLINICAL FINDINGS [Abstract] [Full text]
Dragoslav ?OKIĆ, Marijenka TABAKOVIĆ, Jasmina KNEŽEVIĆ, Bojana MILENKOVIĆ, Dobrivoj POŠTIĆ, Ratibor ŠTRBANOVIĆ, Rade STANISAVLJEVIĆ
VARIABILITY AND CORRELATION BETWEEN PROPERTIES OF MAIZE HYBRID SEEDS OF DIFFERENT FRACTIONS OBTAINED AFTER SEED PROCESSING AND THE INITIAL SEEDLING GROWTH [Abstract] [Full text]
Dušan UROŠEVIĆ, Desimir KNEŽEVIĆ, Gordana BRANKOVIĆ, Aleksandra Yu. NOVOSELSKAYA-DRAGOVICH, Alexander M. KUDRYAVTSEV,Mirela MATKOVIĆ STOJŠIN, Danica MIĆANOVIĆ, Veselinka ZEČEVIĆ
PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.) [Abstract] [Full text]
Hend A. RADWAN, Ahmed I.
ATEYA, Eman A. Abo ELFADL, Shimaa A. SAKR, Mohamed M. FOUDA, Ragab A.
DARWISH, Adel E. EL-DESOKY
EFFICIENCY
OF LEPTIN GENE POLYMORPHISMS IN
THE EVALUATION OF THE GROWTH PERFORMANCE AND CARCASS MEASUREMENTS OF V-LINE
AND BALADI BLACK RABBITS [Abstract] [Full
text]
Snežana GOŠIĆ-DONDO, Jelena SRDIĆ, Vesna DRAGIČEVIĆ, Željko, D. POPOVIĆ, Danijela RISTIĆ, Dragana BRANKOVIĆ, Dragan GRČAK
VARIABILITY OF MAIZE GENOTYPES IN THE EXPRESSION OF TOLERANCE TO EUROPEAN CORN BORER (Ostrinia nubilalis Hbn.) [Abstract] [Full text]
Srećko RAJOVSKI, Nemanja VUČIĆ, Jelena KARANOVIĆ, Suzana MATIJAŠEVIĆ, Dušanka SAVIĆ-PAVIĆEVIĆ, Zorana DOBRIJEVIĆ, Goran BRAJUŠKOVIĆ
ASSOCIATION OF PRMT6, PEX10 AND SOX5 GENETIC VARIANTS WITH IDIOPATHIC MALE INFERTILITY: EVIDENCE FROM NORTH MACEDONIAN POPULATION AND AN UPDATED META-ANALYSIS [Abstract] [Full text]
Hardeep KAUR, Rajinder Kumar DHALL, Priti SHARMA
ASSOCIATION STUDIES IN PEA FOR YIELD AND YIELD ATTRIBUTING TRAITS [Abstract] [Full text]
Yongming FAN, Liuhui YANG, Qi WANG, Jaime A. TEIXEIRA DA SILVA, Xiaonan YU
ASSESSMENT OF GENETIC DIVERSITY IN 55 HERBACEOUS PEONY CULTIVARS WITH SSR MARKERS [Abstract] [Full text]
Pedram AJALLI, Nasser MOHEBALIPOUR, Hasan NOURAFKAN, Hamid HATAMI MALEKI, Ali FARAMARZI
ANATOMICAL VARIABILITY AND POPULATION STRUCTURE OF Cucumis melo L. ACCESSIONS COLLECTED FROM IRAN [Abstract] [Full text]
Habibe Koc UCAR, Fatma Derya BULUT, Berrak Bilginer GURBUZ, Okan DİLEK, Ilker GUNEY
A NOVEL VARIATION OF GAMT IN CEREBRAL CREATINE DEFICIENCY SYNDROME, FIRST COMPLETE HOMOZYGOUS DELETION OF GAMT [Abstract] [Full text] [Supplement]
Ghashia ASIF, Asif NADEEM, Huma MUJAHID, Sana IMRAN, Nadia MUKHTAR, Ali Ahmad SHEIKH, Maryam JAVED
GENOTYPIC DISTRIBUTION OF MSTN GENE POLYMORPHISMS INVOLVED IN RACING PERFORMANCE IN Camelus dromedarius [Abstract] [Full text]
Biljana KORUNOSKA, Vladan PEŠIĆ, Nenad BUNJAC
IDENTIFICATION AND EXAMINATION OF CERTAIN CYTOGENETIC CHARACTERISTICS OF SOME AUTOCHTHONOUS VARIETIES OF GRAPEVINE IN REPUBLIC OF NORTH MACEDONIA [Abstract] [Full text]
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 1-18
© 2023 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.
https://doi.org/10.2298/GENSR2301001M
Original scientific
article
C9orf72 GENETIC SCREENING IN AMYOTROPHIC LATERAL SCLEROSIS PATIENTS FROM SERBIA
Ana MARJANOVIC1,2, Aleksa PALIBRK2, Valerija DOBRICIC2,3 Ognjen MILICEVIC1, Marija BRANKOVIC 1,2, Vanja VIRIC 2, Aleksandra DRINIC4, Gorana MANDIC STOJMENOVIC1,2, Milena JANKOVIC2, Ivana BASTA1,2, Stojan PERIC1,2, Ivana NOVAKOVIC1,2, Elka STEFANOVA1,2, Zorica STEVIC 1,2
1Faculty of Medicine, University of Belgrade, Belgrade, Serbia,
2Neurology Clinic, University Clinical Center of Serbia, Belgrade, Serbia
3Lübeck Interdisciplinary Platform for Genome Analytics, University of Lübeck, Lübeck, Germany
4Institute for Oncology and Radiology of Serbia, Belgrade, Serbia
Abstract
Marjanovic .A., A. Palibrk, V. Dobricic, O. Milicevic, M. Brankovic, V. Viric A. Drinic,, G. Mandic Stojmenovic, M. Jankovic, I. Basta,, S. Peric, I. Novakovic,, E. Stefanova,, Z. Stevic (2023). C9orf72 genetic screening in amyotrophic lateral sclerosis patients from Serbia. - Genetika, Vol 55, No.1, 1-18.
Hexanucleotide repeats expansion in the C9orf72 gene is the most common cause of familial and sporadic amyotrophic lateral sclerosis (ALS) cases in Europe. In this study we aimed to determine the size and distribution of C9orf72 alleles, and investigate the possible association of the repeat size with several clinical parameters in ALS patients from Serbia. Patients were recruited from 2011-2021 and analysed using fragment length analysis and Southern blot. Out of 383 ALS patients, we have detected 31 (8.09%) patients with repeat expansion. In the total ALS cohort, clinical overlap with frontotemporal dementia (FTD) was registered in 17 (4.44%) patients, and among them, 5 (29.41%) were expansion carriers. There was no difference in the age of onset, age at the examination or disease duration, gender, and the frequency of spinal and bulbar onset between patients with and without C9orf72 expansion. The presence of positive family history (34.48% vs. 15.65%) and FTD (16.13% vs. 3.41%) was more frequent in expansion-positive vs. expansion-negative patients. In expansion-positive patients, significantly higher values of the largest detected repeat were found in patients with ALS in the family, and in expansion-negative, a higher median value of the smaller allele was noted in patients with a positive family history of ALS, dementia, and both in comparison to the rest of the group. A correlation of the repeat size was not found with the age of onset in both patients with and without the expansion. This is the first detailed study of C9orf72 sizing in ALS patients from Serbia. Our results emphasize the need for C9orf72 genetic screening in ALS patients with/without FTD.
Key words: C9orf72, repeat expansion, ALS, ALS/FTD, executive dysfunction
Corresponding author: Ana Marjanović, Neurology Clinic, University Clinical Center of Serbia, Doktora Subotica 6,11000 Belgrade, Serbia, ana.marjanovic@yahoo.com, phone:+381113064208
Published in „GENETIKA“ Vol. 55, No. 1 (2023), pp.19-32
© 2023Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 633.11
https://doi.org/10.2298/GENSR2301019E
Original scientific paper
INVESTIGATION OF MOLECULAR VARIABILITY IN SOME Aegilops SPECIES USING START CODON TARGETED POLYMORPHISM (SCoT) AND CAAT-BOX DERIVED POLYMORPHISM (CBDP) MARKERS
Mohammad Reza ESLAMZADEH-HESARI1, Mansoor OMIDI2*, Varahram RASHIDI1,
Alireza ETMINAN3, Alireza AHMADZADEH1
1Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran
3Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
4Department of Plant Breeding and Biotechnology, Shabestar Branch, Islamic Azad University, West Azerbaijan, Iran
Abstract
Eslamzadeh-Hesari R. M., M. Omidi, V. Rashidi, A. Etminan, A. Ahmadzadeh (2023). Investigation of molecular variability in some Aegilops species using Start Codon Targeted Polymorphism (SCoT) and CAAT-Box Derived Polymorphism (CBDP) markers. - Genetika, Vol 55, No.1, 19-32.
Among wild relatives of wheat, Aegilops species are ideal genetic resources for the discovery of new characteristics such as resistance to environmental stresses and even grain quality for wheat improvement. Hence, knowledge of the population structure and genetic diversity of this germplasm is very important for their conservation and further utilization. In the present study, 80 accessions of the Aegilops including Ae. tauschii, Ae. cylindrica and Ae. triuncialis were investigated for genetic diversity using SCoT and CBDP markers. Eight SCOT and twelve CBDP primers amplified a total of 84 and 94 fragments with a mean of 10.50 and 7.83 fragments per primer, respectively. Resolving power (Rp) for SCoT and CBDP primers varied between 6.04 and 11.65, and 13.08 and 28.02, with the polymorphic information content (PIC) from 0.40 to 0.49 and 0.35 to 0.48, respectively. The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred to between species. SCoT primers indicated high values for all informativeness parameter (except resolving power) than CBDP primers across all tested accessions. However, CBDP primers indicated higher values of the genetic parameters than using SCoT primers. As a result, the maximum values for genetic parameters such as number of effective alleles (Ne), Nei’s gene diversity (H) and Shannon’s information index (I) were detected in Ae. cylindrica and Ae. triuncialis using SCoT and CBDP markers, respectively. Cluster analysis based on those molecular system grouped all accessions into three main clusters. The grouping pattern observed by CBDP primers indicated more clear phylogenetic relationship among some Aegilops species, so that PCoA’s results confirmed the grouping pattern. In conclusion, it was observed that SCoT and CBDP displayed good efficiency in depicting polymorphism among the tested accessions, however, CBDP markers provided a clear grouping pattern of evaluated accessions. Hence, the use of CBDP markers in determining population structure and estimating genetic diversity in other plant species is recommended.
Keywords: Aegilops species, gene-targeted markers, PCoA, genetic diversity
Corresponding author: Mansoor Omidi, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran Email: momidi@ut.ac.ir
Telephone number: (+98) 912 564 4435
Published in „GENETIKA“ Vol. 55, No.1 (2023), pp.33-44
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301033T
Original
scientific article
ASSESSMENT OF GENETIC PURITY AND EARLINESS IN F1 AND F2 POPULATION OF CUCUMBER (Cucumis sativus L.) USING SSR MARKERS
Manisha THAKUR1*, Ramesh KUMAR2, Parul SHARMA3, Rajnish SHARMA4
1School of Organic Farming, PAU, Ludhiana, Punjab, India
2Department of Vegetable Science
3,4 Department of Biotechnology, Dr YSP UHF Nauni, Solan, Himachal Pradesh (HP) 173230, India
Abstract
Thakur M., R. Kumar, P. Sharma, R. Sharma (2023). Assessment of genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) using SSR markers. - Genetika, Vol 55, No.1, 33-44.
Simple Sequence Repeat (SSR) markers were used to assess the genetic purity and earliness in F1 and F2 population of cucumber (Cucumis sativus L.) respectively. Experiment was carried out at the Experimental Research Farm of the Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (HP) during the years 2015 and 2016. Experimental material used in the study comprised of Khira-75, a commercial variety grown in low and mid hills of the state (Himachal Pradesh) which is late in flowering and genotype PI-618860, an inbred line collected from North Central Regional Plant Introduction Station, USA which is early in flowering and tolerates various biotic stresses. For assessment of genetic purity and earliness the parents Khira-75 and PI-618860 were crossed and F1 and F2 population were raised. Genetic purity studies revealed that one marker CSN 160 was observed to be segregating in the parents as well as the population. Further, scoring of earliness in F2 population revealed promising results using 2 co-dominant SSR00262 and SSR17922 markers, which segregated in the parents and population in correlation with the observed phenotypes.
Keywords: Assessment; cucumber Cucumis sativus L.; earliness; genetic purity and SSR markers
Corresponding author: Manisha Thakur, School of Organic Farming, PAU, Ludhiana, Punjab, India, E-mail: mnshthakur4@pau.edu
Published in „GENETIKA“ Vol. 55, No1(2023), pp. 45-60
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301045A
Original
scientific article
PERFORMANCE PREDICTION OF F1 CROSSES IN EGGPLANT (Solanum melongena L.) BASED
ON MORPHOLOGICAL AND MOLECULAR DIVERGENCE
Sudheer Kumar ANNEPU1*, Happy Dev SHARMA2, Anupam BARH3, Rajesh DOGRA2,
Vipin SHARMA2, Shivender THAKUR4, Vinay VERMA2 and Kanika SHARMA5
1ICAR-Indian Institute of Soil and Water Conservation, Research Centre, Ooty, Tamil Nadu, India
2Department of Vegetable Science, College of Horticulture, Dr Yashwant Singh Parmar University of Horticulture & Forestry, Nauni, Solan, Himachal Pradesh, India
3ICAR- Indian Institute of Soil and Water Conservation, Dehradun, Uttarakhand, India
4Lovely Professional University, Jalandhar, Punjab, India
5ICAR- Directorate of Mushroom Research, Solan, Himachal Pradesh, India
Abstract
Annepu S. K., H. D.Sharma, A. Barh, R. Dogra, V. Sharma, S. Thakur, V. Verma, K. Sharma (2023). Performance prediction of F1 crosses in eggplant (Solanum melongena L.) based on morphological and molecular divergence. - Genetika, Vol 55, No.1, 45-60.
Identifying potential F1 hybrid combinations based on the parental diversity can increase the breeding efficiency and saves the opportunity cost of time. In this work, the genetic diversity between eggplant genotypes was measured by Mahalanobis D2 statistics and Sequence Related Amplified Polymorphism (SRAP) molecular markers. The genetic distances (GD) were correlated with heterosis and trait wise mean performance of F1 crosses generated in a line × tester mating design for prediction of F1 performance for agronomically important traits. The cluster analysis performed based on the Mahalanobis D2 distance grouped all the eleven genotypes into two clusters and three clusters were formed based on the SRAP marker data. The polymorphic information content value generated by the 30 SRAP marker combinations ranged from 0.09 to 0.77 with a mean value of 0.38. For yield, the F1 combinations exhibited the mid parent heterosis ranged from 3.99% to 83.34% and the heterobeltiosis from -35.67% to 57.19%. GD based on both phenotypic values and molecular marker data successfully predicted the heterotic patterns in the number of fruits per plant and other fruit morphological traits such as fruit length and fruit breadth which is a significant outcome of the study. A multiple linear regression model that included GD, GCA and SCA was more significantly correlated with heterosis for fruit yield than any genetic parameter alone.
Keywords: D2 statistics, Genetic divergence, Heterosis, Multiple linear regression, SRAP markers
Corresponding author: Sudheer Kumar Annepu, ICAR-Indian Institute of Soil and Water Conservation, Research Centre, Ooty, Tamil Nadu, India, E-mail:sudheerannepu@gmail.com: Phone: +91-9849641751
Published in „GENETIKA“ Vol. 55, No.1 (2023), pp. 61-69
© 2023Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301061B
Original
scientific paper
ESTIMATING GENE ACTION AND COMBINING ABILITY FOR YIELD AND FIBER QUALITY IN COTTON (Gossypium hirsutum L.)
Serife BALCI1, Volkan Mehmet CINAR2, Aydin UNAY3*
1Cotton Research Institute, Nazilli-Aydin, Turkey
2Postdoctoral Researcher, Aydin Adnan Menderes University, Faculty of Agriculture, Department of Field Crops, Aydin, Turkey
3Aydin Adnan Menderes University, Faculty of Agriculture, Department of Field Crops,
Aydin, Turkey
Abstract
Balci S, V. M. Cinar, A. Unay (2023). Estimating gene action and combining ability for yield and fiber quality in cotton (Gossypium hirsutum L.). - Genetika, Vol 55, No.1, 61-69.
Genetic evaluation of segregating populations is one of the primary activities of cotton breeders. Seven cotton genotypes were crossed in a line × tester mating design. The population of 12 F1, 12 F2 and their parents were tested in Randomized Complete Block Design with three replications at the Nazilli Cotton Research Institute of Türkiye during the 2014/2015 cotton-growing season. The genetic variability was significant for all characters studied. σ2GCA / σ2SCA indicated non-additive gene action in controlling seed cotton yield (F2), ginning out-turn (F1) and fiber quality traits for both generations. The highly favourable GCA effects for seed cotton yield and fiber strength were recorded in the Carmen cultivar. Carmen × Carisma hybrid exhibited high mean performance and SCA effects for seed cotton yield and fiber strength, while the performance of Gloria × ST-468 was superior in terms of ginning out-turn. We concluded that individual plants with desired traits should be selected in later generations due to the preponderance of non-additive gene actions.
Keywords: Fiber characteristics, GCA, ginning out-turn, line x tester, SCA
Corresponding author: Aydin Unay, Aydin Adnan Menderes University, Faculty of Agriculture, Department of Field Crops, Aydin, Turkey, phone: +90 535 658 5670, E-mail: aunay@adu.edu.tr
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 71-82
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301071G
Original scientific paper
HOTAIR/MIR1 AXIS ACTS AS A POTENTIAL CHEMOTHERAPY TARGET
IN GASTRIC CANCER
Morteza GHANADPOUR, Seyed Reza KAZEMI NEZHAD,
Hamid GALEHDARI, Mohammadreza HAJJARI*
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
Ghanadpour M., Kazemi Nezhad S.R., Galehdari H., Hajjari M. (2023). HOTAIR/miR1 axis acts as a potential chemotherapy target in gastric cancer. - Genetika, Vol 55, No.1, 71-82.
Gastric cancer is one of the most common cancers in the world. Delayed diagnosis is the most common cause of death in patients. Long noncoding RNAs (lncRNAs) are a group of non-coding RNAs that are effective in the incidence of cancers. studies in different cancers determined HOTAIR as an important lncRNA in tumorigenesis. In gastric cancer, the function of HOTAIR in the initiation and progression of cancer seems to be crucial. In this study, we confirmed the significant differential expression of HOTAIR between gastric tumors and normal tissues in different datasets. In the following, the regulatory function of HOTAIR and its interaction with miRNAs in development of gastric cancer was analyzed. Our analysis determined that the upregulation of HOTAIR is essential to different pathways associated with the progression of gastric cancer. Further analysis determined numerous miRNAs as potential targets for HOTAIR. Among them, we demonstrated miR-1 as a significant miRNA with negative correlation with HOTAIR in gastric tumors. Validation analysis determined that HOTAIR is a target of cisplatin as a common chemotherapy drug. Eventually, the effect of cisplatin on the expression of HOTAIR and its potential target, miR-1, was checked by an in vitro study. Cisplatin treatment on the gastric cancer cell line showed that there is a significant negative correlation between the downregulation of HOTAIR and the upregulation of miR-1 in treated cells. In conclusion, comprehensive in silico analysis and experimental study provided evidence for the importance of the HOTAIR/miR-1 axis as potential diagnostic and treatment strategies for gastric cancer.
Keywords: cisplatin, gastric cancer, HOTAIR, miR-1, non-coding RNA
Corresponding author: Mohammadreza Hajjari, Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz 61336-3337, Iran, Tel: +98-6133338965, Fax: +98-6133337009, E-mail: Mohamad.hajari@gmail.com; m-hajari@scu.ac.ir
Published in „GENETIKA“ Vol. 55, No1(2023), pp 83-93
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
Ehttps://doi.org/10.2298/GENSR2301083K
Original
scientific paper
IS THERE AN ADVANTAGE OF MONITORING VIA EXOSOME-BASED DETECTION OF EGFR MUTATIONS DURING TREATMENT IN NON-SMALL CELL LUNG CANCER PATIENTS?
Ege Riza KARAGUR1, Aydin DEMIRAY1, Nedim KARAGENC1, Esra ELVER3,
Onur TOKGUN1, Arzu YAREN2, Gamze G. DOGU2, Hakan AKCA1*
1Department of Medical Genetic, School of Medicine Pamukkale University, Pamukkale, 20160 Denizli, Turkey
2Department of Medical Oncology, Pamukkale University, Pamukkale, 20160 Denizli, Turkey 3Graduate School of Health Sciences, Molecular Cancer Biology, Pamukkale University, Pamukkale, 20160 Denizli, Turkey
Abstract
Karagur E. R., A. Demiray, N. Karagenc, E. Elver, O. Tokgun, A. Yaren, G. G. Dogu, H. Akca (2023). Is there an advantage of monitoring via exosome-based detection of EGFR mutations during treatment in non-small cell lung cancer patients?. - Genetika, Vol 55, No.1, 83-93.
We know that detection of EGFR mutations is very important for individual therapy. Nowadays FFPE samples are commonly using to detect the EGFR mutation status. But it has a few handicaps such as, tumor heterogeneity and non-repeatable, it is need to examine mutation statues of EGFR after each treatment regimen for individually treatment of NSCLC patients. Therefore, there is still need to develop non-invasive and useable over and over again approach for monitoring EGFR mutation statues and other genes for individual therapy. So, we aim to examine whether exosomes are good target for detection of EGFR mutation status or not. Pyrosequencing was used to detect, EGFR mutation in FFPE and exosome samples in some NSCLC patients. For the patients given different chemotherapy regime (n=28), PFS was evaluated before and after treatment. In patients who were EGFR positive before treatment, the median PFS for EGFR mutation-positive patients after treatment was 101.7 weeks (95% CI: 0.09-3.21), while for patients who were negative after treatment, the median PFS was 42.43 weeks (95% CI: 0.31- 10.52). Likewise, in patients who were EGFR negative before treatment and EGFR mutation negative after treatment, the PFS was median 52 weeks (95% CI: 0.17-2.84), while in patients who were positive after treatment, the median PFS was 27.57 weeks (95% CI: 0.35-5.58). We show that exosomes are good tools for monitoring EGFR mutation status and exosomes can be use as semi-invasive method for isolation of tumor DNAs for detection of mutation statues for individually treatment of NSCLC patients.
Keywords: Exosome RNA, Lung cancer, EGFR, NSCLC, PFS
Corresponding author: Hakan Akca, Pamukkale Ünivesitesi Tıbbi Genetik A.D. dekanlık binası kat:3 Pamukkale/Denizli-Turkey, Phone: 0(258) 296 16 68, E-mail: hakca@pau.edu.tr
Published in „GENETIKA“ Vol. 55, No.1 (2023), pp.95-110
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301095O
Original scientific paper
PHYSICOCHEMICAL SUBSTANCES AND BIOACTIVE COMPONENTS OF WILD CORNELIAN CHERRY (Cornus mas L.) FRUITS IN ERZİNCAN PROVINCE
OF EASTERN TURKEY
Koray OZRENK1, Akgul TAS2, Muttalip GUNDOGDU3, Nurhan KESKIN4*, Sezai ERCISLI5
1Siirt University, Faculty of Agriculture, Department of Horticulture, Siirt, Turkey
2Bolu Abant Izzet Baysal University, Seben Izzet Baysal Vocational School, Department of Plant and Animal Production, Bolu, Turkey
3Bolu Abant Izzet Baysal University, Faculty of Agriculture and Natural Sciences, Department of Horticulture, Bolu, Turkey
4Van Yüzüncü Yıl University, Faculty of Agriculture, Department of Horticulture, Van, Turkey
5Ataturk University, Faculty of Agriculture, Department of Horticulture, Erzurum, Turkey
Abstract
Ozrenk K., A. Tas, M. Gundogdu, N. Keskin, S. Ercisli (2023). Physıcochemıcal Substances and bıoactıve components of wıld cornelıan cherry (Cornus mas L.) fruıts ın Erzincan provınce of Eastern Turkey. - Genetika, Vol 55, No.1, 95-110.
Turkey has a very rich flora due to diverse climatic and topographic conditions within the country. Cornelian cherry (Cornus mas L.) is an important wild edible fruits and widely distributed in Turkey and well known for its fruit antioxidants and nutrients. In this study, phytochemical content in fruits of a number of Cornelian cherry genotypes in Erzincan region were determined. The biochemical analysis included organic acids, sugars, vitamin C, antioxidant and individual phenolic compounds. In the study, organic acid content was between 253.09 mg/100 g and 112.50 mg/100 g. Vitamin C content varied between 115.85 mg/100g and 43.77 mg/100 g. Likewise, the sugar content of Cornelian cherry fruits varied from 6.17 g/100 g to 4.06 g/100 g. When the antioxidant content was examined, the highest antioxidant was 980.91 µmol TE/g fresh weight (FW) base and the lowest was 490.38 µmol TE/g FW. In the genotypes examined, gallic acid was determined as the highest among the individual phenolic compounds, while the individual phenolic amounts ranged from 38.93 mg/100 g FW to 4.31 mg/100 g FW. As a result of the study, it was determined that Cornelian cherry fruits are very rich in vitamin C and other phytochemicals and as a result of this examination, 24ER04 and 24ER08 genotypes came to the fore.
Keywords: Cornelian cherry, phenolic compound, organic acid, antioxidant, sugars
Corresponding author: Nurhan Keskin,Van Yüzüncü Yıl University, Faculty of Agriculture, Department of Horticulture, Van, Turkey, e-mail: keskin@yyu.edu.tr
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 111-124
© 2023 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301111R
Original
scientific paper
GENETIC VARIABILITY FOR PLANT GROWTH, FRUIT, SEED
AND BIOCHEMICAL TRAITS IN BELL PEPPER BREEDING POPULATIONS UNDER NORTH INDIAN PLAINS
Menisha RANI*, Salesh Kumar JINDAL, Om Prakash MEENA
Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004, India
Abstract
Rani M., S. K. Jindal, O. P. Meena (2023). Genetic variability for plant growth, fruit, seed and biochemical traits in bell pepper breeding populations under North Indian plains. - Genetika, Vol 55, No.1, 111-124.
The present research aimed to estimate the plant growth, fruit yield, seed and biochemical traits among the seven pepper parental lines and their six BC2F1 populations in order to generate information regarding the extent of genetic variability, heritability and genetic advance. The study was conducted using a randomized complete block design (RCBD) with three replications at the Vegetable Research Farm of Punjab Agricultural University, Ludhiana, India. The analysis of variance (ANOVA) showed significant mean squares due to parents and BC2F1 populations for almost all the studied traits, it indicating the presence of a sufficient amount of inherent variability among the parental line and developed populations which could be exploited via selection. The plant height, fruit weight, fruit width, number of lobes fruit-1, chlorophyll b, total chlorophyll, chlorophyll a and total fruit yield plant-1 were recorded high GCV and PCV among the parental lines and developed populations BC2F1, indicating higher extent of variability for these traits in the evaluated genotypes. The results revealed that traits with high GCV and PCV offer a better scope for improvement through simple selection. High heritability and high estimates of genetic advance were determined for the traits viz., plant height, fruit weight, ascorbic acid content and total fruit yield plant-1, in both parents and populations. Based on these results, we can conclude that these traits are under the additive gene effects and that above mentioned characters could be considered as a good selection indices in bell pepper.
Keywords: Bell pepper, BC2F1 populations, genetic variability, heritability, horticultural traits
Corresponding author: Menisha Rani, Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004, India, E-mail: menisha-vs@pau.edu
Published in „GENETIKA“ Vol. 55, No.1 (2023), pp. 125-140
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
633.11
https://doi.org/10.2298/GENSR2301125M
Original
scientific paper
AMMI ANALYSIS FOR GRAIN YIELD IN BREAD WHEAT RECOMBINANT INBRED LINES
Sajid MUHAMMAD, Sultan AKBAR JADOON*, Fida MOHAMMAD, Iftikhar HUSSAIN KHALIL,
Sheraz AHMAD, Fahim ULLAH KHAN
Department of Plant Breeding and Genetics, Faculty of Crop Production Sciences,
The University of Agriculture, Peshawar-PAKISTAN
Abstract
Muhammad S., S. Akbar Jadoon, F. Mohammad, I. Hussain Khalil, S. Ahmad, F. Ullah Khan (2023). AMMI analysis for grain yield in bread wheat recombinant inbred lines. - Genetika, Vol 55, No.1, 125-140.
Identification of high yielding relatively stable genotypes is an integral objective of plant breeding programs. Testing of genotypes across environments is required to determine yield stability of genotypes. The specific objective of the current study was to analyze genotype by environment interaction (GEI) for grain yield of 44 bread wheat recombinant inbred lines (RILs) and six check cultivars using additive main effect and multiplicative interaction (AMMI) model. Experiments were planted using alpha lattice design with two replicates in Peshawar (E-1 and E-3), Hangu (E-2 and E-4) and Kohat (E-5) Khyber Pakhtunkhwa province, Pakistan. Analysis of variance revealed significant differences among RILs for all traits while interactions due to genotype by environment were significant for all traits except days to emergence and 1000-grain weight. Significant GEI justified environment-specific as well as AMMI analysis to identify RILs with specific and wider adaptation. The AMMI analysis revealed that the first interaction principal component analysis (IPCA 1) captured 64% of GEI sum of squares while the second interaction principal component analysis (IPCA 2) explained 25.8% of the interaction sum of square. The AMMI biplot identified G30 as high yielding genotype followed by G19 and G49, whereas low yielding RILs were G13, G8 and G7. Similarly, G30, being close to IPCA1 axis, was the most stable RIL with wider adaptability followed by G31 and G25. Based on AMMI stability value (ASV), RILs G18 (2.15), G5 (2.78), G27 (3.72), G44 (4.31), G25 (4.43), G42 (4.57), G43 (5.78), G11 (5.82), G1 (7.66) and G29 (7.81) were recognized in the given order of relative stability. Stability analysis identified G49 (Wafaq × Ghaznavi-98-3) as high yielding stable genotype among RILs which can be commercialized after fulfilling procedural requirements.
Key words: AMMI, Bi-plot, Principal components, RILs, Yield stability
Corresponding author: Sultan Akbar Jadoon, Department of Plant Breeding and Genetics, Faculty of Crop Production Sciences, The University of Agriculture, Peshawar-Pakistan, sultan@aup.edu.pk
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 141-158
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301141W
Original
scientific paper
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS
OF NOVEL Wx GENES FROM Psathyrostachys juncea AND Eremopyrum bonaepartis
Lijuan WU2, Zaijun YANG1, Junqiang WANG1,3, Jundong HE1,3, Jin JIANG4, Fang FANG4, Shian SHEN1,3, Xinkun HU1,3*
1Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University, Nanchong, Sichuan, China
2College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, China
3Institute of Ecology, China West Normal University, Nanchong, Sichuan, China
4Nanchong Academy of Agricultural Sciences, Nanchong, Sichuan, China
Abstract
Wu L., Z. Yang, J. Wang, J. He, J. Jiang, F. Fang, S. Shen, X. Hu (2023). Molecular characterization and phylogenetic analysis of novel wx genes from Psathyrostachys juncea and Eremopyrum bonaepartis. - Genetika, Vol 55, No.1, 141-158.
The ratio of amylose/amylopectin can greatly influence the end-use quality of wheat products, and amylose synthesis only needs the granule-bound starch synthase I that is encoded by the Waxy (Wx) gene. Hence, the Wx allele variability is a vital determinant of amylose synthesis. In this study, four novel Wx genes were isolated from two diploid Triticeae species. Their sequence variations were analyzed, which showed that the polymorphism among the Wx-F1 alleles was low; only seven single nucleotide polymorphisms (SNPs) and one amino acid (AA) residue change (Arg541 → Cys) were detected in the exon regions, and this change in Wx-F1b was predicted to have deleterious effects on protein function. The two Wx-Ns1 alleles in this study were different from two published alleles obtained using three overlapping primer sets. The polymorphism among the four Wx-Ns1 alleles was high, and 37 SNPs and ten AA residue changes were found, while all of those substitutions were neutral. However, one substitution (Leu237 → Pro) between Wx-H1 and Wx-Ns1 was predicted that would probably affect the protein function. Divergence time analysis showed that Wx-Ns1a diverged from Wx-Ns1b about 1.06 MYAs. Phylogenetic analysis indicated that Wx-F1 were more closely related to Wx-O1 from Henrardia persica, Wx-R1 from Secale cereale ssp. cereale, and Wx-Xe1 from Eremopyrum triticeum, while Wx-Ns1 were more closely related to Wx-H1 from Hordeum vulgare ssp. vulgare and Wx-Hch1 from Hordeum chilense. These genes may be important candidates for wheat quality improvement.
Keywords: Amylose; Waxy gene; Psathyrostachys; Eremopyrum; Phylogenetic relationships
Corresponding author: Xinkun Hu, Key Laboratory of Southwest China Wildlife Resources Conservation of the Ministry of Education, China West Normal University and Institute of Ecology, China West Normal University, Nanchong, Sichuan, China, Phone: +86-0817-2260685, E-mail: huxinkun123@163.com
Published in „GENETIKA“ Vol. 55, No1 (2023), pp159-176
© 2023 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301159I
Original
scientific paper
CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA
Renata ILIČIĆ1, Aleksandra JELUŠIĆ2, Milan BLAGOJEVIĆ3, Ferenc BAGI1,
Karolina VRANDEČIĆ4, Slaviša STANKOVIĆ5, Tatjana POPOVIĆ MILOVANOVIĆ6*
1University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
2University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
3Academy of Applied Studies, Department of Agricultural and Business Studies and Tourism, Šabac, Serbia
4 Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, Croatia
5University of Belgrade, Faculty of Biology, Belgrade, Serbia
6Institute for Plant Protection and Environment, Belgrade, Serbia
Abstract
Iličić R., A. Jelušić, M. Blagojević, F. Bagi, K. Vrandečić, S. Stanković, T. Popović Milovanović (2023). Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. - Genetika, Vol 55, No.1, 159-176.
Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
Keywords: bacterial leaf spot, stone fruits, diversity, DNA fingerprinting, MLSA
Corresponding author: Tatjana Popović Milovanović, Institute for Plant Protection and Environment, Teodora Drajzera 9, Belgrade, Serbia, Phone: +381112660049, Fax: +381112669860, E-mail: tanjaizbis@gmail.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 177-191
© 2023Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2301177K
Original scientific paper
GENOTYPIC DIFFERENCES FOR CHLOROPHYLL, ASH AND N CONTENTS
AND THEIR RELATIONS WITH GRAIN YIELD IN TURKISH BREAD WHEAT LANDRACES
Rukiye KARA1* and Aydın AKKAYA2
1East Mediterranean Transitional Zone Agricultural Research Institute, Kahramanmaras, Turkey
2Mus Alparslan University, Faculty of Applied Sciences, Department of Plant Production and Technologies, Mus, Turkey
Abstract
Kara R. and A. Akkaya (2023). Genotypic differences for chlorophyll, ash and N contents and their relations with grain yield in Turkish bread wheat landraces. - Genetika, Vol 55, No.1, 177-191.
The genetic differences in the landraces are very important for plant breeding. The aim of this study was to determine the genetic differences for chlorophyll, ash and N contents and their relations with grain yield in Turkish bread wheat landraces. There were significant genetic differences for grain yield (GY), ash content of flag leaf at anthesis (AFLAC), ash content of spike at anthesis (ASAC), ash content of flag leaf at maturity (MFLAC), grain ash content (GAC), chlorophyll content at anthesis (ACC), chlorophyll content at early milk maturity (EMCC), chlorophyll content at late milk maturity (LMCC), chlorophyll content at early dough maturity (EDCC), N content of flag leaf at anthesis (FLN) and spike N contents (SN). The grain yield was positively and significantly related with AFLAC, ASAC, MFLAC and ACC, negatively and significantly related with GAC, EDCC and SN, not significantly related with EMCC, LMLC and FLN.
Keywords: ash, chlorophyll, nitrogen contents, wheat landraces, yield
Corresponding author: Rukiye Kara, East Mediterranean Transitional Zone Agricultural Research Institute, Kahramanmaras, Turkey, E-mail: rkara46@tarimorman.gov.tr
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 193-202
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2301193P
Original scientific paper
FRUIT CHARACTERISTICS OF PROMISING WALNUT GENOTYPES FROM THE REGION OF EASTERN SERBIA
Svetlana M. PAUNOVIĆ*, Rade K. MILETIĆ
Fruit Research Institute, Čačak, Republic of Serbia
Abstract
Paunović S., R. Miletić (2023). Fruit characteristics of promising walnut genotypes from the region of eastern Serbia. - Genetika, Vol 55, No.1, 193-202.
This research was conducted to examine the fruit characteristics of selected walnut genotypes from the native population of Eastern Serbia. Over 28 trees that deserve attention were recorded through the selection procedure. By grafting, grouping and cultivation under the same conditions at the Fruit Research Institute in Čačak, seven promising genotypes with the most favourable fruit characteristics were identified. Important physical properties of the fruit, such as fruit weight, kernel weight, fruit dimensions and some kernel and shell properties were investigated. The fruits of the selected genotypes were large or very large. The fruit weight ranged from 13.4 to 17.9 g, whereas the kernel weight varied from 6.22 to 8.92 g. The kernel was light-coloured or yellow, of very good taste. The kernel percentage in the tested genotypes was high, over 50.0% (50.7–55.3%). Pearson's correlation coefficient revealed a significant correlation between fruit and kernel characteristics. PCA analysis showed an adequate grouping of genotypes based on fruit quality. The obtained results suggest that selected promising walnut genotypes showed very good fruit properties, and may contribute to the increase of walnut production in the future.
Keywords: Juglans regia L., genotype, fruit properties
Correspondin
author: Svetlana M. Paunović, Fruit Research
Institute, Čačak, Republic of Serbia, Kralja Petra I, 932 000 Čačak, Serbia,
Phone: +381 32 321 375, Fax: +381 32 321 391, E-mail: svetlana23869@gmail.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 203-215
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2301203A
Original scientific paper
ESTIMATION OF HETEROSIS, HETEROBELTIOSIS, AND GENE ACTION
IN F1 PLANTS FROM SMALL-SEEDED SOYBEAN CROSSES
M. Muchlish ADIE1*, Arifin ARIFIN2, Suyamto SUYAMTO1, Ayda KRISNAWATI1
1Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Indonesia
2Indonesian Legume Instrument Standard Testing, Indonesia
Abstract
Adie M. M., A. Arifin, S. Suyamto, A. Krisnawati (2023). Estimation of heterosis, heterobeltiosis, and gene action in F1 plants from small-seeded soybean crosses. - Genetika, Vol 55, No.1, 203-214.
Soybeans with small seed sizes and high seed yield potential are crucial for addressing the needs of soybean-based industries. The objectives of the study were to investigate the maternal effect and quantify the value of heterosis, heterobeltiosis, and gene action in F1 plants from soybean crosses with small-seeded. In the present investigation, the experiment was conducted in the screen house of the Indonesian Legume and Tubers Crop Research Institute (ILETRI) located in Malang (East Java, Indonesia) from March to July 2021. The F1 plants were derived from crosses of Lokal Jember × Tidar, Tidar × Gepak Kuning, Gepak Kuning × Detam 3, and their reciprocals. The parental genotypes and F1 plants were all planted. The observed data were plant height, number of branches, number of nodes, number of filled pods, the weight of 10 seeds, and seed yield/plant. The performance of the F1 plants showed variation for all observed traits, which is mostly averaged between the two parents, including 10 seed weight and seed yield. There was no maternal effect for almost all agronomic traits on all crosses and their reciprocals, except for the number of nodes in the cross of Gepak Kuning × Detam 3. Heterosis (hMP) and heterobeltiosis (hHP) of F1 derived from six cross combinations for seven agronomic traits ranged from -25.93 to 186.27 and -94.83 to 114.71, respectively. Based on the potence ratio, the gene action was differed from a cross to cross for seed yield and its components. The degree of dominance for seed size and seed yield varied from overdominance, incomplete dominance, and complete dominance. Thus, based on the present investigation, it is possible to develop soybean varieties with small seed sizes and high yield by using pedigree selection on F2.
Keywords: Agronomic character, degree of dominance, maternal effect, reciprocal crosses, yield
Corresponding author: M. Muchlish Adie, Research Center for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), Indonesia, email: mm_adie@yahoo.com, +6281333220026
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 217-228
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301215S
Original
scientific paper
MOLECULAR CHARACTERIZATION OF Dittrichia viscosa (L.) GREUTER (ASTERACEAE) POPULATIONS REVEALED BY ISSR MARKERS AND CHLOROPLAST (cpDNA) trnL INTRON SEQUENCES
Emre SEVINDIK1*, Makbule BOZKURT1, Mehmet YILMAZ1, Ezginur ŞENYÜZ1,
Mehmet Yavuz PAKSOY2
1*Aydın Adnan Menderes University, Faculty of Agriculture, Department of Agricultural Biotechnology, Aydın, Turkey
2Department of Medical Services and Techniques, Tunceli Vocational School, Munzur University, Tunceli, Turkey
Abstract
Sevindik E., M. Bozkurt, M. Yilmaz, E. Şenyüz, M. Y. Paksoy (2023). Molecular characterization of Dittrichia viscosa (L.) greuter (Asteraceae) populations revealed by ISSR markers and chloroplast (CPDNA) tRNL intron sequences- Genetika, Vol 55, No.1, 217-228.
In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Aydın province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 ISSR primers were used to determine the molecular characterization among the populations. For cpDNA trnL intron amplification, trnC and trnD primers were used. In ISSR analysis, a total of 70 bands were obtained. The polymorphism rate was determined to be approximately 94.28%. According to the ISSR analysis, the UPGMA dendrogram consisted of three groups. For cpDNA trnL intron sequences, phylogenetic analyses were obtained along with genetic distances. For populations, cpDNA trnL intron sequences were determined between 454 and 472 bases. The maximum likelihood phylogenetic tree consist of two clades. In addition, cpDNA trnL intron sequences of some species (Chrysophthalmum Pulicaria Inula Jasonia Stenachaenium Carpesium Blumea Iphiona Limbarda Rhanterium Lifago Duhaldea Chiliadenus) of Asteraceae family from NCBI with Dittrichia viscosa populations phylogenetic tree was constructed. As a result of the study, it was determined that the polymorphism rate of Dittrichia viscosa populations obtained by ISSR-PCR was higher than the results obtained from trnL intron sequences.
Keywords: Dittrichia viscosa, ISSR-PCR, cpDNA trnL intron, molecular
Corresponding author: Emre Sevindik, Department of Agricultural Biotechnology, College of Agriculture, Aydın Adnan Menderes University, South Campus, Cakmar-Aydin, Turkey, E-mail: ph.d-emre@hotmail.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp.229-244
© 2023 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301229S
Original scientific paper
TEXT-11 SNPS AND NEIGHBORING SEQUENCES ROLE IN CAUSING MALE INFERTILITY IN SOME ETHNIC GROUPS OF IRANIAN AND THEIR POTENTIAL ROLE IN ESTIMATING DIVERGENCE TIME
Masoud SHEIDAI1*, Sogol ALLAHVERDI2, Narges ANVARI2, Somayeh EIVAZI KHAMENE2, Mozhgan GHANDEHARI ALAVIJEH2, Marziyeh SEYED GHOREISHI2, Fatemeh JAMSHIDI2, Ghazal KHOSRAVANI2, Naser KALHOR3, Fahimeh KOOHDAR1
1Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3Department of mesanchymal stem cell, Academic Center for Education, Culture and Research Qom branch, Iran
Abstract
Sheidai M., S. Allahverdi, N. Anvari, S. Eyvazi Khamene, M. Ghandehari, M. Ghoreshi, F. Jamshidi, G. Khosravani, N. Kalhor, F. Koohdar (2023). Text-11 snps and neighboring sequences role in causing male infertility in some ethnic groups of Iranian and their potential role in estimating divergence time- Genetika, Vol 55, No.1, 229-244.
X-chromosome and its independent loci recently gained importance in genetic investigations concerned with disease control-case studies, population genetic analyses, and phylogenetic investigations. Infertility and lack of normal reproduction are important issues in health. Aazoospermia is a kind of infertility in men whose semen contains no sperm and can affect about 1-20% of the male population. The majority of cases of spermatogenic failure in humans are idiopathic, and the underlying causes are postulated to be genetic. We analyze the potential association between seven X-Chromosome SNPs and the neighboring sequences with azoospermia in four ethnic populations in Iran. We also carried out population genetic analyses to produce data on genetic variability, population structure, gene flow, and time of divergence in these ethnic groups, for the first time. As the X-chromosome genes/ SNPs are in homozygous condition, for the association study we used multivariate statistical methods, which revealed the potential role of variable sites close to the studied SNPs in male infertility. Haplotype groups were identified for each of the seven SNPs. The results showed gene flow among ethnic groups due to migration and inter-breeding and Bayesian analysis of sequence data suggested divergence time for ethnic populations to be between 2-30 KY.
Keywords: Azoospermia, Population, SNPs, X-chromosome
Corresponding author: Masoud Sheidai, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, Email: msheidai@yahoo.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp245-262
© 2023 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
630
https://doi.org/10.2298/GENSR2301245M
Original
scientific paper
PROGRESS, ADAPTABILITY AND STABILITY OF SOYBEAN GRAIN YIELD AND GRAIN QUALITY IN CONVENTIONALLY CREATED ELITE LINES
Maja MATOŠA KOČAR1*, Aleksandra SUDARIĆ1,2, Tomislav DUVNJAK1,
Zoe ANDRIJANIĆ2,3, Antonela MARKULJ KULUNDŽIĆ1 ;
1Agricultural Institute Osijek, Osijek, Croatia
2Centre of Excellence for Biodiversity and Molecular Plant Breeding, University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
3Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
Abstract
Matoša Kočar M., A. Sudarić, T. Duvnjak, Z. Andrijanić, A. Markulj Kulundžić (2023). Progress, adaptability and stability of soybean grain yield and grain quality in conventionally created elite lines.- Genetika, Vol 55, No.1, 245-262.
The objective of this study was to determine the progress in grain yield and grain quality accomplished with conventional breeding methods, as well as to identify stable, widely or specifically adapted genotypes under central European growing conditions. Recently developed soybean elite lines of maturity groups (MGs) 00, 0 and I were compared with commercial cultivars (standards) in comparative field tests during three consecutive years (2018-2020) in Osijek, Croatia. The ANOVA results showed significant genotype, environment, and genotype-by-environment interaction effects. There was a significant improvement in productivity and quality in comparison to standards, while stability parameters for tested traits indicated there are stable and mostly specifically adaptable elite lines. Improvement of the domestic gene pool and high agronomic performances of elite lines stable in most important economic traits will considerably contribute to increasing and improving soybean production in central Europe.
Keywords: eco-valence, grain yield, grain quality, soybean, stability
Corresponding author: Maja Matoša Kočar, Agricultural Institute Osijek, Južno predgra?e 17, 31 103 Osijek, Croatia, E-mail: maja.matosa@poljinos.hr
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 263-275
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301263D
Original
scientific paper
THE EFFECT OF EPISTATIC INTERACTIONS BETWEEN GENETIC VARIANTS LOCATED IN MICRORNA AND SILENCING COMPLEX GENES ON PROSTATE CANCER PROGRESSION RISK
Zorana DOBRIJEVIĆ1,2, Jelena KARANOVIĆ1, Dušanka SAVIĆ-PAVIĆEVIĆ1,
Goran BRAJUŠKOVIĆ1*
1University of Belgrade - Faculty of Biology, Belgrade, Serbia
2Present address: University of Belgrade – Institute for Application of Nuclear Energy, Belgrade, Serbia
Abstract
Dobrijević Z., J. Karanović, D. Savić-Pavićević, G. Brajušković (2023). The effect of epistatic interactions between genetic variants located in microRNA and silencing complex genes on prostate cancer progression risk.- Genetika, Vol 55, No.1, 263-275.
Previous studies conducted in Asian and European populations have provided evidence of the association between microRNA-related genetic variants and prostate cancer (PCa) risk and/or progression. Nevertheless, the results obtained in these studies are inconsistent, which could be explained by the limitations of single-locus main effect evaluations to detect joint effects of multiple genetic variants, reflected in statistical epistases. Therefore, we conducted the analysis of potential epistatic interactions between variants located in microRNA genes and in genes encoding the components of RNA-induced silencing complex (RISC) in relation with PCa risk/aggressiveness. Raw data on genotyping results from our previous studies involving four microRNA polymorphisms and five variants in RISC genes were subjected to the exclusion of samples based on missing data criterion, followed by the re-evaluation of Hardy-Weinberg equilibrium. Afterwards, these genotyping results were included in the Multifactor dimensionality reduction (MDR) analysis. Permutation testing was conducted in order to assess statistical significance of the best models from MDR tests. MDR tests on the risk of developing PCa yielded statistically insignificant results. Nevertheless, the MDR results for comparison of PCa patients with high and low cancer progression risk were statistically significant for the analysis that included rs11614913, with the 3-locus best model comprising this genetic variant, rs7813 and rs784567. We conclude that statistical epistasis between rs11614913 in hsa-miR-196a2, rs7813 in GEMIN4 and rs784567 in TARBP2 shows association with the invasiveness of PCa.
Keywords: epistasis; MDR, microRNA, prostate cancer, RISC
Corresponding author: Goran Brajušković, University of Belgrade - Faculty of Biology, Belgrade, Serbia, Phone: + 38162279777; Fax: + 381112639100, E-mail: brajuskovic@bio.bg.ac.rs
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 277-288
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301277D
Original
scientific paper
ASSOCIATION OF MICRO RNA EXPRESSIONS WITH PEDIATRIC CELIAC CLINICAL FINDINGS
Guzide DOGAN1*, Seda ORENAY BOYACIOGLU2, Metin CALISKAN3, Elmas KASAP4, Semin AYHAN5, Erhun KASIRGA6
1Department of Pediatric Gastroenterology, Faculty of Medicine, Bezmialem University, Istanbul, Turkey
2Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, Turkey
3Department of Medical Biology, Faculty of Medicine, Usak University, Usak, Turkey
4Department of Gastroenterology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey
5Department of Pathology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey
6Department of Pediatric Gastroenterology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey
Abstract
Dogan G., S. Orenay Boyacioglu, M. Caliskan, E. Kasap, S. Ayhan, E. Kasirga (2023). Association of micro RNA expressions with pediatric celiac clinical findings.- Genetika, Vol 55, No.1, 277-288.
There is a need to determine the relationship between the function of the immune system and miRNA expression in pediatric celiac disease (pCD). We aimed to describe the expression profiles of miRNAs in Turkish pCD patients based on the clinical and pathological findings. This study was conducted on 33 pCD patients and 33 pediatric control subjects with normal biopsy results. Four most common mutations (DQA1*05, DQB1*02, DQA1*03, DQB1*03:0.2) on HLA gene in pCD were screened. Paraffin-embedded biopsy tissue samples were used in miRNA isolations followed by cDNA synthesis. Expression of miRNAs were evaluated in the groups with qRT-PCR array-method. Significant underexpression of hsa-miR-194-5p gene was detected in pCD patients compared to the control group. The hsa-miR-194-5p gene was significantly underexpressed in anemic or short stature pCD patients compared to the control. The genes of hsa-miR-29b-3p, hsa-miR-30e-5p, and hsa-miR-146a-5p were significantly overexpressed in the patients with constipated celiac patients. Significant overexpression of hsa-miR146a-5p gene was detected in the Marsh2 and Marsh3a groups. The hsa-miR-29b-3p, hsa-miR-30e-5p, hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR141-3p, hsa-miR143-3p, and hsa-miR-146a-5p miRNA genes were significantly overexpressed in the Marsh3b group. Also, the hsa-miR-194-5p and hsa-miR-26a-5p genes were significantly underexpressed in the comparison of Marsh3c group to the control. These results suggest that miRNA expressions are likely to play a role in the pathogenesis of pCD. It is believed that the current results present valuable inferences that may help understand the genetic boundaries on pCD, which might be further supported by follow up studies on other miRNAs.
Keywords: Autoimmunity; chronic enteropathy; epigenetics; miRNA; pediatric celiac disease
Corresponding author: Guzide Dogan, Department of Pediatric Gastroenterology, Bezmialem Vakıf University, İstanbul, Turkey, Tel: +90 5058219424, E-mail:guzidedogan@gmail.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 289-300
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2301289D
Original scientific article
VARIABILITY AND CORRELATION BETWEEN PROPERTIES OF MAIZE HYBRID SEEDS OF DIFFERENT FRACTIONS OBTAINED AFTER SEED PROCESSING AND THE INITIAL SEEDLING GROWTH
Dragoslav ?OKIĆ1 *, Marijenka TABAKOVIĆ 2*, Jasmina KNEŽEVIĆ3, Bojana
MILENKOVIĆ3, Dobrivoj POŠTIĆ4, Ratibor ŠTRBANOVIĆ4, Rade STANISAVLJEVIĆ4
1 Faculty of Agriculture in Krusevac, University of Nis, Kruševac, Serbia
2 Maize Research Institute, Zemun Polje, Belgrade-Zemun, Serbia
3 Faculty of Agriculture, Lešak, University of Priština, Serbia
4 Institute for Plant Protection and Environment, Beograd, Serbia
Abstract
?okić D., M. Tabaković, J. Knežević, B. Milenković, D. Poštić, R. Štrbanović, R.Stanisavljević (2023). Variability and correlation between quality of maize hybrid seeds of different fractions obtained after seed processing and the initial seedling growth.- Genetika, Vol 55, No.1, 289-300.
The shape and the size of maize seeds are the most variable traits, which are determined by a genotype and environmental conditions. The aim of this study was to understand the effects of the mechanism of the relationship and significance of seed variability on germination and morphology of seedlings. The seeds of five hybrids ZP388, ZP434, ZP555, ZP606, and ZP6263 were used in this study. The following seed traits were analysed: physical ones: seed length (L), thickness (T) and the width (W); morphological ones: seed weight (SW), seedling length (SLW), root length (RL), shoot length (SL) and seed germination (G) as a phydiological trait.There are statistically significant differences not only among physical traits of the seeds of the five hybrids (p≤0.05), but also among the morphological traits (p≤0.05). Statisticlly significant differences (p≤0.05). in the width (W), length(L) and thickness (T) of seeds of all hybrids were determined in the small flat fraction (SP). The large rounded seed fraction (KO) mainly differed in the width and thickness between hybrids ZP434 and ZP 555, while the large flat seed fraction differed the most in the length between these two hybrids. Large-flat (KP) seed fractions are also characterized by the highest germination (99%). As the seed weight increases, the seedling weight decreases (R2=0.527). Segmentation within hybrids according to the diversity of morphological and physiological properties of seeds was carried out according to the seed size, fraction and seedling weight, while the other parameters were less important. The characteristic of all hybrids is that large seeds of the KP fraction have high germination and well-developed seedlings.
Keywords: Zea mays, seed fractions, seed size, germination, seedling
Corresponding author: Marijenka Tabaković, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade-Zemun, Serbia, mtabakovic@mrizp.rs, djdragosla01@gmail.com
Published in „GENETIKA“ Vol. 55, No1(2023), pp.301-318
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2301301U
Original
scientific paper
PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT
(Triticum aestivum L.)
Dušan UROŠEVIĆ1, Desimir KNEŽEVIĆ2, Gordana BRANKOVIĆ3,
Aleksandra Yu. NOVOSELSKAYA-DRAGOVICH4, Alexander M. KUDRYAVTSEV4,
Mirela MATKOVIĆ STOJŠIN5, Danica MIĆANOVIĆ6, Veselinka ZEČEVIĆ7
1Maize Research Institute Zemun Polje, Zemun Serbia
2University of Priština, temporary settled in Kosovska Mitrovica, Faculty of Agriculture, Lešak, Lešak, Kosovo and Metohija, Serbia
3University of Belgrade, Faculty of Agriculture Zemun, Belgrade, Serbia
4Russian Academy of Sciences Laboratory of Plant Genetics, “N.I.Vavilov” Institute of General Genetics RAS, 119333 Moscow, Russia
5Tamiš Research and Development Institute, Pančevo, Serbia
6Serbian Chamber of Commerce and Industry, Bеlgrade, Serbia
7Institute for Vegetable Crops Smederevska Palanka, Smederevska Palanka, Serbia
Abstract
Urošević, D., D. Knežević, G. Branković, A.Yu. Novoselskaya-Dragovich, A.M. Kudryavtsev, M. Matković Stojšin, D. Mićanović, V. Zečević (2023): Protein content and amino acid composition in seed of bread wheat (Triticum aestivum L.).- Genetika, Vol 55, No.1, 301-318.
The aim of this study was determination of protein content and amino acid composition in seeds of bread wheat, with particular focus of evaluation essential amino acids (EAAs). For analysis used flour samples of grained seed of 10 wheat variety, which selected in different breeding center (in Novi Sad and Kragujevac, Serbia). Kjeldahl method was used for determination of nitrogen (N) contents which value multiplied with coefficient 5.7 for computing protein content (protein contents = 5.7 x % N contents). Amino acids analyses of wheat samples were performed by ion exchange chromatography, followed by the ninhydrin color reaction and photometric detection at 570 nm and 440 nm (for proline). The results showed that the mean protein content for wheat varied from the lowest value 10.24% in Ljubičevka to the highest 14.21% in Fortuna variety. The mean contents (g 100 g-l protein) of nonessential amino acids (NEAAs) were aspartic acid 5.42%, serine 4.23%, glutamic acid 18.51%, proline 12.18%, glycine 4.17%, alanine 3.64%, tyrosine 2.52%, arginine 5.02%, while mean contents of essential amino acids (EAAs) were threonine 2.86%, valine 4.52%, methionine 1.28%, isoleucine 3.87%, leucine 5.87%, phenylalanine 4.62%, histidine 2.46%, lysine 2.91%. The variety Ljubičevka contained relatively high concentration of the most deficient EAAs (threonine, valine, leucine, lysine,). The highest value of total essential amino acids (TEAA 31.88 g 100 g-l protein), of total nonessential amino acids (TNEAA 61.96 g 100 g-l protein) as well as the highest value of total amino acids (TAA = 90.84 g 100 g-l protein) were found in Ljubičevka. Obtained results provide variability of wheat variety on the base of amino acid contents and indicate reliable variety for selecting desirable parents in breeding program for improving nutrient quality.
Keywords: amino acid composition, amino acid score protein content
Corresponding author: Dušan Urošević, Maize Research Institute Zemun Polje, Slobodana Bajića 1. 11080 Zemun Serbia; e-mail: dusan.urosevic@gmail.com
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp. 319-338
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301319R
Original
scientific paper
EFFICIENCY OF LEPTIN GENE POLYMORPHISMS IN THE EVALUATION OF THE GROWTH PERFORMANCE AND CARCASS MEASUREMENTS OF V-LINE AND BALADI BLACK RABBITS
Hend A. RADWAN1, Ahmed I. ATEYA1*, Eman A. Abo ELFADL1, Shimaa A. SAKR1,
Mohamed M. FOUDA1, Ragab A. DARWISH1, Adel E. EL-DESOKY2
1Department of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
2Department of Waterfowls and Rabbits Researches, Animal Production Research Institute, Dokki, Giza, Egypt
Abstract
Radwan H. A., A. I. Ateya, E. A. Abo Elfadl, S. A. Sakr, M. M. Fouda, R. A. Darwish, A. E. El-Desoky (2023). Efficiency of leptin gene polymorphisms in the evaluation of the growth performance and carcass measurements of v-line and Baladi black rabbits.-Genetika, Vol 55, No.1, 319-338.
This study investigated the association between both polymorphisms and metabolic marker changes of the leptin gene, and body weight, weight gain, carcass traits, feed intake, and feed conversion ratio. Blood samples were collected from 60 V-line and 60 Baladi Black rabbits for DNA extraction and biochemical analysis. DNA sequencing of leptin (202-bp) revealed four non-synonymous single nucleotide polymorphism (SNPs) that characterized a number of V-line rabbits. Statistical analysis revealed that the identified SNPs were associated with rabbit growth and carcass measurements (p < 0.05). A discriminant analysis model showed a high classification percentage for the identified SNPs within and between breeds using body weight at 5–14 weeks of age (91.7%), slaughter traits (91.6%), feed intake, daily feed intake, and feed conversion ratio (76.7%). This study reveals that leptin gene could be a candidate for growth traits in rabbits enabling the development of marker-assisted selection (MAS) in different rabbit breeds.
Keywords: Carcass measurements, discriminant analysis, growth performance, leptin gene, rabbit, SNP.
Corresponding author: A. I. Ateya, Department of Animal Husbandry and Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Egypt, ahmed_ismail888@yahoo.com Tel: +2-01003541921, FAX: +2-050-2372592
Published in „GENETIKA“ Vol. 55, No. 1(2023), pp 339-353
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2301339G
Original
scientific paper
VARIABILITY OF MAIZE GENOTYPES IN THE EXPRESSION OF TOLERANCE
TO EUROPEAN CORN BORER (Ostrinia nubilalis Hbn.)
Snežana GOŠIĆ-DONDO1*, Jelena SRDIĆ1, Vesna DRAGIČEVIĆ1,
Željko, D. POPOVIĆ2, Danijela RISTIĆ1, Dragana BRANKOVIĆ1, Dragan GRČAK3
1Maize Research Institute, Zemun Polje, Belgrade
2University of Novi Sad, Faculty of Science Novi Sad, Serbia
3 University of Priština, Faculty of Agriculture, Kosovska Mitrovica – Lešak, Lešak,
Serbia
Abstract
Gošić Dondo, S., J. Srdić, V. Dragićević, Ž: D: Popović, D. Ristić, D. Grčak (2023): Variability of maize genotypes in the expression of tolerance to European corn borer (Ostrinia nubilalis Hbn.).- Genetika, Vol 55, No.1, 339-353.
The backbone of modern technologies is the creation of tolerant genotypes at the ECB with special emphasis on the interaction between the degree of plant damage and metabolic processes as potential responses to stress. During the vegetation period of 2016 and 2018, the variability and response of four maize genotypes to the effectiveness of insecticides and bioinsecticides and the interdependence with the dynamics of the ECB population were examined. Out of a total of 11,023 attacked plants in both years of the survey, in 2016 the largest number of plants (4,841) with a broken stalk above the ear and at the height of the ear and belonged to categories C3 to C8. In 2018, there was no damage to the tassel and ear, while 3,672 plants were dominated by leaf damage and perforation in the H2 type. In addition to the fact that more infested plants were observed in the genotypes, ZP 434 and ZP 666 the categories of damage type C5, C6 and C7 were more pronounced. The foliar treatment of plants with chlorantraniliprole in two-year research proved to be the most effective with the least number of attacked plants with a high degree of damage. In contrast, bioinsecticide treatment of plants, in addition to a large number of infested plants, was dominated by high categories of damage that deviated from all other treatments as well as from control. Within the definition of the degree of tolerance of maize genotypes, the influence of treatment on changes in secondary metabolites (phytins) in seeds was observed. It was noticed that there is a trend of positive correlation between the amount of phytin in the seed (mg g-1) with the attack of moths of the first generation and the number of dissected larvae, as well as a trend of negative correlation with the percentage of attacks of the second generation. Correlation coefficients are expressed: in the genotype ZP-600, while in the genotype ZP-666 correlation is the lowest, ie. irrelevаnt. The obtained results may be of great importance in future work aimed at the selection of maize genotypes tolerant and resistant to corn borer attack, connected to the role of phytin, as an antioxidant and phosphorus deposited in corn grain.
Keywords: maize genotypes, tolerance, Ostrinia nubilalis
Corresponding author: Snežana Gošić-Dondo,Maize Research Institute, Slobodana Bajica1, Zemun Polje, Belgrade, e-mail: sgosic@mrizp.rs
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 355-372
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.
https://doi.org/10.2298/GENSR2301355R
Original
scientific paper
ASSOCIATION OF PRMT6, PEX10 AND SOX5 GENETIC VARIANTS WITH IDIOPATHIC MALE INFERTILITY: EVIDENCE FROM NORTH MACEDONIAN POPULATION AND AN UPDATED META-ANALYSIS
Srećko RAJOVSKI1, Nemanja VUČIĆ2, Jelena KARANOVIĆ2, Suzana MATIJAŠEVIĆ2, Dušanka SAVIĆ-PAVIĆEVIĆ2, Zorana DOBRIJEVIĆ3*, Goran BRAJUŠKOVIĆ2*
1Private healthcare institution Clinical Hospital “Acibadem Sistina”, Skopje, North Macedonia
2University of Belgrade – Faculty of Biology, Centre for Human Molecular Genetics, Belgrade, Serbia
3University of Belgrade – Institute for the Application of Nuclear Energy, Department for Metabolism, Belgrade, Serbia
Abstract
Rajovski S., N. Vučić, J. Karanović, S. Matijašević, D. Savić-Pavićević, Z. Dobrijević, G. Brajušković (2023). Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: evidence from north Macedonian population and an updated meta-analysis.-Genetika, Vol 55, No.1, 355-372.
PRMT6, PEX10 and SOX5 genetic variants were identified as male infertility-associated loci in a genome-wide association study and further validated in various populations. Still, the results of previous case-control studies varied, which could be due to differences in participants’ ethnic backgrounds. The main purpose of the present study was to evaluate the supposed association of these variants with idiopathic male infertility in North Macedonian population. Furthermore, we aimed to conduct the systematic quantitative data synthesis which includes the results of previous studies on the same issue in other European and non-European populations. A total of 137 men from North Macedonia diagnosed with idiopathic infertility and 130 age-matched fertile controls were included in the present case-control study. PCR-RFLP method was used for genotyping. Meta-analysis was performed by OpenMeta-analyst statistical software. Variants rs10842262 in SOX5, rs2477686 in PEX10 and rs12097821 in PRMT6 showed the lack of statistically significant differences in genotype distributions between men diagnosed with idiopathic infertility and the control group. Still, rs10842262 allele G frequency was significantly increased in men with poor sperm concentration (P= 0.024, OR = 2.10, 95%CI 1.08–4.06). Meta-analysis further showed the association of rs10842262 and rs12097821 with the risk of idiopathic male infertility. Our results obtained in North Macedonian population supported the previous reports on the involvement of rs10842262 in the genetic basis of male infertility. The meta-analysis confirmed the association of rs10842262 and rs12097821 with male infertility occurrence. Still, additional studies are needed to support the present findings.
Keywords: male infertility, rs10842262, rs12097821, rs2477686, meta-analysis
Corresponding author: Zorana Dobrijević, University of Belgrade – Institute for the Application of Nuclear Energy, Department for Metabolism, Belgrade, Serbia. Tel: +381 11 2618 666, E-mail: zorana.dobrijevic@inep.co.rs
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 373-383
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301373K
Original
scientific paper
ASSOCIATION STUDIES IN PEA FOR YIELD AND YIELD ATTRIBUTING TRAITS
Hardeep KAUR1, Rajinder Kumar DHALL1, Priti SHARMA2
1Department of Vegetable Science, Collage of Horticulture and Forestry, Punjab Agricultural University, Ludhiana-141004, Punjab, India
2School of Agricultural Biotechnology, Collage of Agriculture, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Abstract
Kaur H., R. Kumar Dhall, P. Sharma (2023): Association studies in pea for yield and yield attributing traits.- Genetika, Vol 55, No.1, 373-385.
The present study was conducted to find out correlation and path analysis for 10 yield and yield attributing traits using 159 diverse pea genotypes. Correlation studies revealed that the days to 50% flowering, node at which 1st pod appears, number of pods/plant, number of seeds/pod, pod length, pod weight and days to 1st picking had significant and positive correlation with total yield/plant while, plant height and shelling percentage were negatively correlated with total yield/plant. The inter-correlation was observed between days to 50% flowering, days to 1stpicking and node at which 1stpod appears; shelling percentage with number of seeds/pod and pod length; plant height with number of pods/plant and days to 1st picking. It is concluded that there is positive and inter-correlation of total yield/plant with the number of pods/plant, pod length, number of seeds/pod, days to 50% flowering, days to 1stpicking. The positive association suggested that selection should be oriented towards more number of pods per plant, pod length, more number of seeds per pod which would consequently leads to higher pod yield per plant. The Path analysis studies revealed that the number of pods per plant exhibit highest positive effect on yield per plant.
Keywords: Correlation, path analysis, garden pea, traits, yield
Corresponding author: Rajinder Kumar Dhall, Department of Vegetable Science, Collage of Horticulture and Forestry, Punjab Agricultural University, Ludhiana-141004, Punjab, India, Е-mail: rajinderkumar@pau.edu
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 387-400
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301387F
Original
scientific paper
ASSESSMENT OF GENETIC DIVERSITY IN 55 HERBACEOUS PEONY CULTIVARS WITH SSR MARKERS
Yongming FAN1, 2, Liuhui YANG1, 2, Qi WANG1, 2, Jaime A. TEIXEIRA DA SILVA3,
Xiaonan YU1, 2*
1College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P.R. China
2Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Beijing, 100083, P.R. China
3Independent researcher, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan
Abstract
Fan Y., L. Yang, Q. Wang, J.A. Teixeira Da Silva, Xiaonan Yu (2023): Assessment of genetic diversity in 55 herbaceous peony cultivars with SSR markers.- Genetika, Vol 55, No.1, 387-400.
Using SSR, a molecular marker, this study aimed to evaluate the genetic diversity and genetic relationship among 55 herbaceous peony (Paeonia lactiflora Pall.) cultivars belonging to the Lactiflora, Hybrid and Itoh Groups to lay a foundation for the molecular classification of peony cultivars, as well as for genetic background analysis and breeding of new cultivars. Our results show that 86 alleles, 76 of which were polymorphic, were obtained by screening 13 pairs of polymorphic primers from 100 initially tested primers, each of them having an average of 6.615 alleles. The range of variation of the effective alleles was 2.243–7.800, PIC content was 0.554–0.872 and Shannon’s genetic diversity index was 1.701–3.126. The 55 herbaceous peony cultivars were divided into nine groups based on UPGMA. This analysis indicates that the 55 cultivars have rich genetic diversity while a phylogenetic relationship of most cultivars was identified. This molecular-based classification is a useful and simple reference molecular method to differentiate peony cultivars in crossbreeding programs.
Keywords: Cluster analysis; Genetic diversity; Herbaceous peony; SSR molecular marker
Corresponding author: Xiaonan Yu, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China, E-mail: yuxiaonan626@126.com
Published in „GENETIKA“ Vol. 55 No.1(2023), pp. 401-414
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/GENSR2301401A
Original scientific
paper
ANATOMICAL VARIABILITY AND POPULATION STRUCTURE OF Cucumis melo L. ACCESSIONS COLLECTED FROM IRAN
Pedram AJALLI1, Nasser MOHEBALIPOUR1*, Hasan NOURAFCAN2,
Hamid HATAMI MALEKI3, Ali FARAMARZI1
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
2 Department of Horticultural Sciences, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
3 Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh,
Marageh, Iran
Abstract
Ajalli P., N. Mohebalipour, H. Nourafkan, A. Faramarzi, H. Hatami Maleki (2023): Anatomical variability and population structure of Cucumis melo L. accessions collected from Iran.- Genetika, Vol 55, No.1, 401-414.
Melon (Cucumis melo) is one of the most important cultivated cucurbits. In this project, the genetic variability among 14 accessions of melon collected by the authors has been studied using 23 anatomical characteristics across 2 consecutive years as well as 146 random amplified polymorphic DNA (RAPD) markers. Combined analysis of variance revealed significant effects on genotype and year × genotype effects. Among examined anatomical characters, the highest CV% values across two years belonged to fruit store at room temperature (88.96%, 103.6%) and total fruit weight (42.2%, 40.00%), while the lowest CV% values were observed in flower petal width (0.31%, 0.21%), flower petal length (0.28%, 0.49%), and peduncle length (0.40%, 0.19%). Classification of melon accessions based on anatomical characteristics using the Ward method produced three groups. The highest Jaccard’s similarity coefficient (0.76) was observed between accessions “Atashi koluche” and “Atashi miyaneh” and the lowest value (0.49) was found between accessions “Bakermellon” and “Mashhadi” with the mean value of 0.59. In this study, OPA06 and OPB13 primers possessed greater efficiency in the genetic evaluation of the studied germplasm. Analysis of population structure, which imply on the existence of admixture in the studied melon germplasm, revealing three subpopulations. Accession “Sabzevari” identified as mixed subgroups. From the breeder’s view, the introduced heterotic groups can be utilized in parental selection for the construction of mapping population and the identified population structure can prevent any false positive output in marker-trait association studies of melon.
Keywords: melon, RAPD, heterotic groups, population structure
Corresponding author: Nasser Mohebalipour, Deapartment of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran n.mohebalipour@m-iau.ac.ir
Published in „GENETIKA“ Vol. 55, No.1(2023), pp. 415-426
© 2023Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301415U
Original scientific paper
A NOVEL VARIATION OF GAMT IN CEREBRAL CREATINE DEFICIENCY SYNDROME, FIRST COMPLETE HOMOZYGOUS DELETION OF GAMT
Habibe Koc UCAR1,*, Fatma Derya BULUT2, Berrak Bilginer GURBUZ2, Okan DİLEK3,
Ilker GUNEY4
1Department of Pediatric Neurology, Ministry of Health, Adana City Training and Research Hospital, Adana, Turkey
2Depatment of Metabolism and Nutrition, Ministry of Health, Adana City Training and Research Hospital, Adana, Turkey
3Depatment of Radiology, Ministry of Health, Adana City Training and Research Hospital, Adana, Turkey
4 Department of Genetics, Ministry of Health, Adana City Training and Research Hospital, Adana, Turkey
Abstract
Ucar H. K., F. D. Bulut, B. B. Gurbuz, O. Dilek, I. Guney (2023): A novel variation of gamt in cerebral creatine deficiency syndrome, first complete homozygous deletion of GAMT.- Genetika, Vol 55, No.1, 415-426.
Cerebral Creatine Deficiency Syndromes (CCDS) are congenital metabolic disorders in the creatine metabolism pathway. In this study, we evaluated the clinical, phenotypic, radiological and genetic features of patients with CCDS. We tried to identify early diagnosis clues in patients. Especially, we reviewed the causes of delay in patients with late diagnosis.
In line with these findings, the diagnosis is confirmed by enzyme tests and next generation sequencing based whole genome sequencing. In this study, 6 patients whose diagnosis was genetically confirmed were presented (5 GAMT mutations (someone is complete homozygous deletion in GAMT gene), 1 SLC6A8 mutation). 5 of these patients were from the same family, and 4 patients were patients with a late diagnosis. Two of the 4 patients who were diagnosed late were moderate and two had severe phenotype. The neurological findings consisted of patients with different clinical findings such as speech disorder, cognitive retardation, autism and epilepsy. Patients received appropriate treatment for the type of cerebral creatine deficiency.
While response to treatment was good in early diagnosed cases, a partial clinical improvement was detected in cases diagnosed late. The patient, who was started treatment before neurological symptoms appeared, was neurodevelopmentally normal. It was observed that there was a strong relationship between age at diagnosis and phenotype and prognosis.
We compared the clinical findings, phenotype and genotype characteristics of patients with CCDS. We reviewed the causes of delay in patients with late diagnosis. Thus, we wanted to raise awareness about early diagnosis and treatment of CCDS, one of the rare metabolic diseases.
Keywords: autism, cerebral creatine deficiency; epilepsy, guanidinoacetate methyltransferase, SLC6A, cognitive impairment
Corresponding author: Habibe Koc Ucar, MD.,Adana City Training and Research Hospital, Department of Pediatric Neurology,01220, Yüreğir, Adana/Turkey,Phone: +90 506 5146605,E-mail: hkocselanik@gmail.com ; drfizyoloji@gmail.com
Published in „GENETIKA“Vol. 55, No.1(2023), pp. 427-437
© 2023 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2301427A
Original
scientific paper
GENOTYPIC DISTRIBUTION OF MSTN GENE POLYMORPHISMS INVOLVED
IN RACING PERFORMANCE IN Camelus dromedarius
Ghashia ASIF1$, Asif NADEEM2$, Huma MUJAHID1, Sana IMRAN1, Nadia MUKHTAR3,
Ali Ahmad SHEIKH3, Maryam JAVED1*
1Institute of Biochemistry & Biotechnology, University of Veterinary & Animal Sciences Lahore-Pakistan
2Department of Biotechnology, Virtual University of Pakistan
3Institute of Microbiology, University of Veterinary & Animal Sciences, Lahore-Pakistan
Abstract
Asif G., A. Nadeem, H. Mujahid, S. Imran, N. Mukhtar, A. A. Sheikh, M. Javed (2023): Genotypic distribution of mstn gene polymorphisms involved in racing performance in Camelus dromedarius.- Genetika, Vol 55, No.1, 427-437.
Camel racing is one of the popular sports around the world and is growing rapidly especially in Gulf countries. Camel has adapted itself to harsh and draught climate of desert. Тhis quality of camel makes it the best choice in racing industry. Pakistani breed Dromedarius camels are as good as Omani and Sudanese camel breeds in their racing potential. Myostatin (Growth differentiation factor 8) is a protein coded by MSTN gene. Polymorphism in MSTN play a significant role in growth of muscle, increasing fast glycolysis Type IIB muscle fiber, forming tubular aggregates in type IIB fiber by increasing the body strength and racing ability. This gene is also responsible for double muscle phenotype in bovines. In present study, Marecha and Brela camel breeds of Pakistan were studied for genomic characterization of MSTN gene. Blood samples were collected from the healthy animals between the age group of 2-4 years. Genomic DNA was extracted, amplified by using specific sets of primers, purified and sequenced by Sanger’s dideoxy chain termination method. No single nucleotide polymorphisms (SNPs) were found in the exon-3 of MSTN of Marecha & Brela, which shows that the gene is highly conserved among species while phylogenetic data of the dromedarius MSTN gene showed highest similarity with Bos taurus and least similarity with Gallus gallus. Genes that are similar to the camel MSTN are myostatin of Homo sapiens, Pan troglodytes, Bos taurus, Canis lupus, Rattus norvegicus. The significance of this study was to identify the genetic potential of Pakistani camel for racing that will help in the socioeconomic uplift of the local community. It will also help the camel breeders to select the best breed of camel & enhance their genetic potential by using least operating cost. That will in turn provide opportunity to the camel breeders to produce the best breed that will be selected by the trainers for racing that will provide another source of income for the local community and Pakistan’s camels will also be used in the racing industry.
Keywords: Camelus Dromedarius, conserved gene, MSTN, SNP, muscle mass
Corresponding author: Maryam Javed, Sheikh Abdul Qadir Jillani Raod, University of Veterinary & Animal Sciences, Lahore, Pakistan, 54000. Phone# +92-347-4462376, E-mail: maryam.javed@uvas.edu.pk
Published in „GENETIKA“Vol. 55, No1(2023), pp. 439-454
© 2023 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2301439K
Original scientific paper
IDENTIFICATION AND EXAMINATION OF CERTAIN CYTOGENETIC CHARACTERISTICS OF SOME AUTOCHTHONOUS VARIETIES
OF GRAPEVINE IN REPUBLIC OF NORTH MACEDONIA
Biljana KORUNOSKA1*, Vladan PEŠIĆ2, Nenad BUNJAC2
1*University „Ss.Cyril and Methodius“- Skopje, Institute of Agriculture, Skopje, R.N. Macedonia
2, 3 University of Belgrade, Faculty of Agriculture, Beograd-Zemun, R. Serbia
Abstract
Korunoska B., V. Pešić, N. Bunjac (2023): Identification and examination of certain cytogenetic characteristics of some autochthonous varieties of grapevine in Republic of North Macedonia.- Genetika, Vol 55, No.1, 437-452.
In R.N. Macedonia has a large number of autochthonous or domestic varieties (cultivars) of grapevine that are grown as individual vines or in small, limited plantations. Until the beginning of the 20th century and before the phylloxera attack in our country, native varieties were much more represented. Autochthonous varieties existed and were cultivated for a long time in several vineyards and were characterized by stable and significant properties. These varieties are adapted to the environmental conditions where they are grown and are carriers of stable genes that can be used in further selection and hybridization to obtain new grape varieties. Therefore, it is very important for a country (region) to have autochthonous varieties in its assortment.In this paper, we considered four autochthonous grape varieties - two table varieties (Konchanka and white winter) and two wine varieties (Ohrid white, Ohrid black). We tried, according to the ampelographic description and certain characteristics of the reproductive system, to determine their origin. According to the phenotypic characteristics that refer to the centers of origin of the varieties, the studied varieties - Konchanka and White winter belong to the Eastern varieties convarietas orientalis, subconvarietas antasiatica, and the studied varieties Ohrid white and Ohrid black variety belong to the Black Sea - Balkan varieties convarietas. pontica, subconvarietas balcanica. Among the examined varieties, the properties from the second group of descriptors are described in detail according to the proposals of O.I.V. According to some codes (characteristics) there is a big difference between varieties. Also, for a more detailed differentiation of the varieties, cytological properties were examined - pollen germination and vitality, number of chromosomes in mitosis and meiosis, ploidy level, percentage of fertilization (fecundation), self-fertilization (autogamy) and cross-fertilization (xenogamy). The statistical processing of the ampelography data is according to a cluster program (SPSS) that works on the binary similarity or dissimilarity as measures used to group the data and further form a clear dendogram display.
Keywords: autochthonous varieties, ampelographical description, chromosomes, cytological properties
Corresponding author: Biljana Korunoska, University „St.Cyril and Methodius“- Skopje, Institute of Agriculture, Str. „16 Makedonska brigada“No. 3A 1000, Skopje, R.N. Macedonia, E-mail: markovskabiljana@yahoo.com