GENETIKA, Vol. 56, No.2 (2024)
Verónica GRANADOS-FLORES, Rafael VILLALOBOS-PIETRINI, Víctor M. SALCEDA, Judith JIMÉNEZ-GUZMÁN, Judith GUZMÁN-RINCÓN
CLIMATE CHANGE EFFECT ON THE GENETICS OF A MEXICAN POPULATION OF Drosophila pseudoobscura [Abstract] [Full text]
Tanveer KAUR, Rajinder Kumar DHALL, Pooja MANCHANDA, Priyanka KUMARI
UTILIZING GYNOECIOUS CUCUMBER (CUCUMIS SATIVUS L.) INBREDS TO INVESTIGATE INHERITANCE OF GYNOECIUM, FRUIT YIELD AND ITS CONTRASTING TRAITS [Abstract] [Full text]
Ioannis Ipsilantis, Evaggelos Korpetis, Ioannis Tsialtas
SOME AGRONOMIC TRAITS AFFECTING BARLEY MYCORRHIZATION, GRAIN YIELD
AND QUALITY [Abstract] [Full
text] [Supplement]
Reena KUMARI, Ramesh KUMAR, Rajeev KUMAR, Aanchal CHAUHAN
GENERATION MEAN ANALYSIS OF UNADAPTED LANDRACES AND WILD RELATIVES OF CUCUMBER (Cucumis sativus L.) FOR EARLINESS AND DOWNY MILDEW [Abstract] [Full text]
Kemalcan OKAN Zekiye ÖZKARA, Emre SEVİNDİK, Mustafa SEVİNDİK, Mehmet Yavuz PAKSOY
MOLECULAR MARKERS ANALYSIS OF ENDEMIC BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) IN TÜRKİYE [Abstract] [Full text]
Hasan PINAR, Halit YETIªIR, Alim AYDIN, Merve A. YIGIT, Abdullah ULAª, Hamide AYDIN
DETERMINATION OF IN VITRO SALT TESTING EFFICIENCY AND SALINITY TOLERANCE OF DIFFERENT PEPPER (Capsicum annum L.) GENOTYPES [Abstract] [Full text]
A.M.EMAM, Maysoon M.MAKHLOUF, Reem. S.MOURAD
MICROSATELLITE MARKERS APPLICATION IN THE GENETIC SURVEY OF NATIVE RABBITS IN THE EGYPTIAN DELTA [Abstract] [Full text]
Zeynab MAMMADOVA, Ikram RUSTAMOV, Narinj RUSTAMOVA, Gular IBRAHIMOVA, Mehraj ABBASOV, Sevda BABAYEVA
STUDY OF NEUROPILIN-2 RS849563 GENE POLYMORPHISM IN CHILDREN WITH AUTISM SPECTRUM DISORDER [Abstract] [Full text]
Valentin KOSEV, Viliana VASILEVA, Vera POPOVIÆ
NEW VARIETY OF WHITE LUPINE MONICA (Lupinus
albus L.) [Abstract] [Full
text]
Alexander KRIVORUCHKO, Andrey LIKHOVID, Anastasiya KANIBOLOTSKAYA, Olga KRIVORUCHKO, Larisa SKORYKH, Natalia KIZILOVA, Olesya YATSYK
GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING [Abstract] [Full text] [Supplement]
Published in „GENETIKA“ Vol. 56, No2(2024), pp. 243-253
© 2024 Serbian Genetics Society
S. Bajiæa 1, 11185 Belgrade-Zemun
UDC 575.
https://doi.org/10.2298/GENSR2402243G
Original scientific article
CLIMATE CHANGE EFFECT ON THE GENETICS OF A MEXICAN POPULATION OF Drosophila pseudoobscura
Verónica GRANADOS-FLORES1, Rafael VILLALOBOS-PIETRINI1, Víctor M. SALCEDA2*, Judith JIMÉNEZ-GUZMÁN3, Judith GUZMÁN-RINCÓN1.
1Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán 04510. México D.F.
2Departamento de Biología, Instituto Nacional de Investigadores Nucleares. Centro Nuclear de México, Km. 36.5 Carretera México Toluca. Salazar, Edo. de México, México.
3Departamento de Ciencias de la Alimentación, Universidad Autónoma Metropolitana, Unidad Lerma. Av. Hidalgo Poniente No.46, Lerma de Villada, Edo. De México, México.
Abstract
Granados-Flores V., R. Villalobos-Pietrini, V. M. Salceda, J. Jiménez-Guzmán, J. Guzmán-Rincón (2024). Climate change effect on the genetics of a Mexican population of Drosophila pseudoobscura. - Genetika, Vol 56, No.2, 243-253.
The chromosomal polymorphism present in populations of seveal species of Drosophila could be related with changes in time or ecological parameter. The aim of this work is to evaluate the long-term effect of climatic changes on the frequencies of D. pseudoobscura chromosomal arrangements. The population studied is located in a forest near Tulancingo, México, the chromosomal analysis has been studied for more than 35 years. The relative frequencies found in that period were used to evaluate the changes. The climatic data of the National Meteorological Service were gather for the period from 1974 to 2011. A multiple analysis of variance was use to analyze the relationship between the changes in the chromosomal arrangement frequencies and the different climatic variables (maximum and minimum temperature, evaporation and pluvial precipitation). Results showed a significant correlation of several chromosome gene arrangements with one or more hydro-climatic variables modifying the genetic structure of the natural population. Comparisons with other populations shown and allow the establishment of the effect of climate change on the genetic structure of natural populations.
Keywords: Inversion polymorphism, D. pseudoobscura
Corresponding author: Víctor M. Salceda, Departamento de Biología, Instituto Nacional de Investigadores Nucleares. Centro Nuclear de México, Km. 36.5 Carretera México Toluca. Salazar, Edo. de México, México, E-mail: victor.salceda@inin.gob.mx
Published in „GENETIKA“ Vol. 56, No.2 (2024), pp.255-269
© 2024 Serbian
Genetics Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/ GENSR2402255K
Original scientific article
UTILIZING GYNOECIOUS CUCUMBER (CUCUMIS SATIVUS L.) INBREDS
TO INVESTIGATE INHERITANCE OF GYNOECIUM, FRUIT YIELD AND ITS CONTRASTING TRAITS
Tanveer KAUR1, Rajinder Kumar DHALL1*, Pooja MANCHANDA, Priyanka KUMARI1
1Department of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India
2School of Agricultural Biotechnology, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab, India
Abstract
Kaur T., R. Kumar Dhall, P. Manchanda ,P.Kumari (2024). Utilizing gynoecious cucumber (cucumis sativus l.) inbreds to investigate inheritance of gynoecium, fruit yield and its contrasting traits. - Genetika, Vol 56, No.2, 255-269.
To determine the inheritance of fruit yield and its component traits in cucumber, three commercial monoecious varieties (Pusa Uday, Punjab Naveen and Pant Kheera-1) were crossed with gynoecious parent (Gy-14) and developed six generations of each cross. The scaling test and the three-parameter model of the joint scaling test revealed the presence of epistasis for most of the traits. Duplicate epistasis was found in the majority of the traits, which will decrease the net gain from heterozygosity because of epistatic effects and dominance cancellation. The fruit weight in Gy-14 × Pant Kheera-1 showed a higher additive × dominance type among epistatic interactions, while fruit length in all crosses and fruit breadth in Gy-14 × Pant Kheera-1 showed a higher additive × additive type of interaction, indicating the use of pedigree method to improve these traits. Fruit diameter, fruit weight, number of fruits per plant and yield per plant in Gy-14 ×Punjab Naveen, and fruit weight in Gy-14 × Pusa Uday were found to exhibit a higher dominance × dominance interaction. Consequently, these traits can be further enhanced through heterosis breeding in the corresponding crosses.
Keywords: Cucumber, Epistatis, Gene effects, Generation mean analysis, Gynoecious
Published in „GENETIKA“ Vol. 56, No.2 (2024), pp.271-281
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630.11
https://doi.org/10.2298/
GENSR2402271I
Original scientific article
SOME AGRONOMIC TRAITS AFFECTING BARLEY MYCORRHIZATION,
GRAIN YIELD AND QUALITY
Ioannis Ipsilantis1*, Evaggelos Korpetis2, Ioannis Tsialtas3
1Aristotle University of Thessaloniki, Faculty of Agriculture, Lab. of Soil Science, 541 24 Thessaloniki, Greece
2Hellenic Agricultural Organization-«Demeter», Institute of Plant Breeding and Genetic Resources, 570 01 Thermi, Greece
3Aristotle University of Thessaloniki, Faculty of Agriculture, Lab. of Agronomy, 541 24 Thessaloniki, Greece
Abstract
Ipsilantis I., E. Korpetis, I. Tsialtas (2024). Some agronomic traits affecting barley mycorrhization, grain yield and quality. - Genetika, Vol 56, No.2, 271-281.
Breeding barley (Hordeum vulgare L.) for low-input conditions may be a key factor for enhancing yields in poor environments. Arbuscular mycorrhizal (AM) symbiosis and seeding rate may also affect barley performance in alkaline, low-P soils under Mediterranean conditions. For two growing seasons, two conventionally bred and two cultivars bred under low-input conditions were tested at three seeding rates (300, 400 and 500 seeds m-2) under rainfed Mediterranean conditions. Length of root colonized by AM fungi and plant height were determined at anthesis, whereas grain yield (GY), 1000-kernel weight (TKW) and protein concentration (PC) were measured at harvest. Across the growing seasons, GY was highest (2713.6 kg ha-1) at the highest seeding rate. The shorter, conventionally bred cultivars yielded better compared to the low-input-bred counterparts (2872.6 vs. 2228.1 kg ha-1). However, the low-input cultivars had significantly higher PC (12.63 vs. 12.04%). The six-row cultivars were more productive compared to two-row ones (2854.1 vs. 2246.6 kg ha-1) with higher TKW (40.22 vs. 35.99 g). No differences between cultivars, seeding rates or breeding method were found for AM colonization of roots. Low-input breeding did not select for higher mycorrhization and did not perform better than conventionally bred barley cultivars under low-input conditions.
Keywords: Hordeum vulgare, low-input agriculture, mycorrhizae, phosphorus, seeding rate
Corresponding author: Ioannis Ipsilantis, Aristotle University of Thessaloniki, Faculty of Agriculture, Lab. of Soil Science, 541 24 Thessaloniki, Greece, phone number (+30) 2310998746, E-mail: iipsi@agro.auth.gr
Published in „GENETIKA“ Vol. 56, No2(2024), pp. 283-293
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.829
https://doi.org/10.2298/
GENSR2402283K
Original scientific article
GENERATION MEAN ANALYSIS OF UNADAPTED LANDRACES AND WILD RELATIVES OF CUCUMBER (Cucumis sativus L.) FOR EARLINESS AND DOWNY MILDEW
Reena KUMARI*, Ramesh KUMAR, Rajeev KUMAR, Aanchal CHAUHAN
Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni-173230, Solan (Himachal Pradesh), India
Abstract
Kumari R., R. Kumar, R. Kumar, A. Chauhan (2024). Generation mean analysis of unadapted landraces and wild relatives of cucumber (Cucumis sativus L.) for earliness and downy mildew. - Genetika, Vol 56, No.2, 283-293.
The choice of breeding method for the genetic improvement of any crop largely depends on understanding the genetics and inheritance patterns of the traits involved. The knowledge pertaining to gene actions and interactions is likely to direct and strengthen the crop breeding programmes. With this objective the present investigation aims to elucidate the nature and magnitude of gene action associated for various earliness and disease trait through generation mean analysis (GMA) with six generations (P1, P2, F1, F2, B1 and B2) of two crosses involving three diverse parents of cucumber. The studies were undertaken at Experimental Research Farm of the Department of Vegetable Science, Dr YSP and UH&F Nauni, Solan, HP (India) under open field conditions. Experimental results revealed that non-allelic interactions were present for all the traits considered in both the crosses as evident from individual scaling and joint scaling test. In the inheritance of most of traits except a few, both additive and non-additive gene actions contribute significantly therefore, the breeding methods reciprocal recurrent selection/ bi-parental mating could be exploited for the improvements of the traits. Earliness traits viz., node number bearing first female flower and days to first fruit set was mainly governed by dominance (h) and hence these characters can be improved through heterosis breeding. The duplicate type of epistasis influenced all traits in both cross-combinations; indicating the mild selection intensity in the earlier generations while intense in later generations.
Keywords: Bi-parental mating, duplicate epistasis, joint scaling test, non-allelic interactions, reciprocal recurrent selection, Scaling
Corresponding author: Reena Kumari, Department of Vegetable Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni-173230, Solan (Himachal Pradesh), India, Mobile No: +91 98175 55455, E-mail: reena.sarma92@gmail.com
Published in „GENETIKA“ Vol. 56, No.2 (2024), pp. 295-304
© 2024Serbian
Genetics Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/
GENSR2402295O
Original
scientific article
MOLECULAR MARKERS ANALYSIS OF ENDEMIC BORNMUELLERA HAUSSKN. SPP. (BRASSICACEAE) IN TÜRKİYE
Kemalcan OKAN1, Zekiye ÖZKARA1, Emre SEVİNDİK1*, Mustafa SEVİNDİK2,3,
Mehmet Yavuz PAKSOY4
1Department of Agricultural Biotechnology, Faculty of Agriculture, Aydın Adnan Menderes University, South Campus, Cakmar, Aydin, Türkiye
2 Department of Biology, Faculty of Engineering and Natural Sciences, University of Osmaniye Korkut Ata, Osmaniye, 80000, Türkiye
3 Department of Life Sciences, Western Caspian University, Baku, Azerbaijan
4Department of Medical Services and Techniques, Munzur University, Tunceli Vocational School, 62000 Tunceli, Türkiye
Abstract
Okan K., Z. Özkara, E. Sevindik, M. Sevindik, M. Y. Paksoy (2024). Molecular markers analysis of endemic Bornmuellera hausskn. spp. (Brassıcaceae) ın Türkiye. - Genetika, Vol 56, No.2, 295-304.
In this study, molecular characterisation of Türkiye's endemic species Bornmuellera cappadocica (Willd.) Cullen & T.R. Dudley, Bornmuellera glabrescens (Boiss. & Balansa) Cullen & T.R.Dudley, Bornmuellera kiyakii Aytaç & Aksoy and Bornmuellera angustifolia (Hausskn. ex Bornm.) Cullen & T.R.Dudley was carried out using ten RAPD and ten ISSR primers. In RAPD-PCR analysis, 66 bands were obtained and the polymorphism rate was 96.96%. In the ISSR-PCR analysis, 119 bands were obtained and the polymorphism rate was 95.79%. In the UPGMA (Unweighted Pair Group Method with Arithmatic Mean) dendrogram based on RAPD-PCR, B. angustifolia and B. glabrescens were found to be a sister group, and B. kiyakii and B. cappadocica were a sister group. Principal Component Analysis (PCA) analysis based on RAPD-PCR were compatible with the UPGMA dendrogram. In the UPGMA dendrogram based on ISSR-PCR, B. kiyakii and B. glabrescens were found to be sister groups, and B. cappadocica was closely related to this group. PCA analysis based on ISSR-PCR were compatible with the UPGMA dendrogram. As a result, both RAPD and ISSR results a high rate of polymorphism were obtained. The results were compared with previous sequence-based studies, morphological, anatomical and palynological studies.
Keywords: Bornmuellera, RAPD, ISSR, molecular markers, Türkiye
Corresponding author: Emre Sevindik, Department of Agricultural Biotechnology, Faculty of Agriculture, Aydın Adnan Menderes University, South Campus, Cakmar, Aydin, Türkiye, E-mail: ph.d-emre@hotmail.com
Published in „GENETIKA“ Vol. 56, No. 2(2024), pp. 305-319
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 630
https://doi.org/10.2298/
GENSR2402305P
Original
scientific article
DETERMINATION OF IN VITRO SALT TESTING EFFICIENCY AND SALINITY TOLERANCE
OF DIFFERENT PEPPER (Capsicum annum L.) GENOTYPES
Hasan PINAR1, Halit YETIªIR1, Alim AYDIN2, Merve A. YIGIT1, Abdullah ULAª3,
Hamide AYDIN 1
1Erciyes University, Faculty of Agriculture, Department of Horticulture, 38280 Kayseri, Turkey
2Kırºehir Ahi Evran University Faculty of Agriculture, Department of Horticulture, 40100 Kırºehir, Turkey
3Erciyes University, Faculty of Agriculture Department of Soil Sci. and Plant Nutrition, 38280 Kayseri, Turkey
Abstract
Pinar H., H. Yetiºir, A. Aydin, M. A. Yigit, A. Ulaº, H. Aydın (2024). Determination of in vitro salt testing efficiency and salinity tolerance of different pepper (Capsicum annum L.) genotypes. - Genetika, Vol 56, No.2, 305-319.
This study was conducted to determine salt-tolerance levels of different pepper genotypes and to determine of correlation the efficiency of in vitro and hydroponic culture salt-testing procedures. For this purposes, 7 different pepper genotypes were subjected to control (0 mM) and 5 different NaCl levels 50, 100, 125, 150 mM in in vitro and hydroponic culture to determine salt-tolerance. Different pepper genotypes exhibited different responses to different NaCl doses and significant correlations were observed between in vitro and hydroponic culture testing for some of the investigated parameters. In vitro germination had the significant correlation with Shoot Fresh Weight (0.80) and Root Dry Weight (0.85). On the other hand, in vitro plant height highly correlated with hydroponic NaCl testing parameters and had the highest correlation with Root Dried Weight (0.71). There were also high correlations between in vitro root length and hydroponic NaCl testing parameters such Shoot Fresh Weight (0,84), Root Dry Weight (0.85) and Leaf Area (0.77). Present findings proved that in vitro salt-testing was a simple and cheap method. Thus, it could be preferred by the breeders just to get reliable outcomes in a short time.
Keywords: Pepper, Salt screening, Hydroponic culture, Efficiency
Corresponding author: Alim Aydin, Kırºehir Ahi Evran University Faculty of Agriculture, Department of Horticulture, 40100 Kırºehir, E-mail:alim.aydin@ahievran.edu.tr
Published in „GENETIKA“ Vol. 56, No2(2024), pp 321-336
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/
GENSR2402321E
Original
scientific article
MICROSATELLITE MARKERS APPLICATION IN THE GENETIC SURVEY OF NATIVE RABBITS IN THE EGYPTIAN DELTA
A.M. EMAM1 *, Maysoon M. MAKHLOUF1, Reem S. MOURAD2
1 Animal Production Research Institute, Agricultural Research Centre, Ministry of Agriculture, Nadi El Saiid street, 12618, Dokkii, Giza, Egypt.
2 Department of Animal Production, Faculty of Agriculture, Menoufia University, Egypt
Abstract
Emam A.M., M.M. Makhlouf, R.S. Mourad (2024). Microsatellite markers in the genetic survey of native rabbits in the Egyptian delta. - Genetika, Vol 56, No.2, 321-336.
The human interest in exotic animal breeds in the agricultural sector led to the deterioration of local breeds. The interest in national farm animal genetic studies is important for the agriculture ecosystems under climate change challenges. Microsatellite markers are important tools to determine the genetic status of breeds, populations, and subpopulations. In this study, 28 microsatellite loci were used to investigate the genetic situation among 274 biological samples collected from the native Delta Egypt rabbits (NDER) population in the north of Egypt. They belonged to eight subpopulations (Damietta, Dakahlia, Kafr El sheikh, Beheira, Gharbia, Menoufia, Sharqia, and Qalyubia). It was found that expected heterozygosity (He) values were greater than observed heterozygosity (Ho). A total of 184 alleles were identified, with a mean of 6.571 and 4.122 as effective alleles. About 89% of microsatellite markers expressed high informative values in the polymorphism information content (PIC). The comparison among 8 NDER subpopulations showed low genetic variability parameters with high inbreeding coefficient (FIS) values in the north (Damietta, Dakahlia, Kafr El sheikh, Beheira, and Gharbia). However, values of genetic variables increased with decreasing FIS in the middle (Menoufia), east (Sharqia), and south (Qalyubia) Delta. Furthermore, the discriminant analysis principal components (DAPC) showed overlaying in the north. In the same context, the neighbor-joining tree (NJ) and heatmap showed the genetic convergence among the northern subpopulations. The analysis of STRUCTURE found 4 clusters (K= 8). The north subpopulations were in one cluster, while others in the middle, east, and south were a separate cluster for each subpopulation. Our findings show that the NDER suffers from genetic drift in the northern Delta subpopulations. On the contrary, the south, east, and middle subpopulations showed more genetic variability. A strategy of correct mating should be fostered to improve the genetic traits of rabbits.
Keywords: Native Rabbits, Delta, Subpopulation, Egypt, Microsatellite
Corresponding author: Emam A.M., Animal Production Research Institute, Agricultural Research Centre, Ministry of Agriculture, Nadi El Saiid street, 12618, Dokkii, Giza, Egypt. Tel: +201113779271, Fax: +20233372934, E-mail: ahmed.emam@arc.sci.eg
Published in „GENETIKA“ Vol. 56, No.2 (2024), pp.337-345
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/
GENSR2402337M
Original
scientific article
STUDY OF NEUROPILIN-2 RS849563 GENE POLYMORPHISM IN CHILDREN
WITH AUTISM SPECTRUM DISORDER
Zeynab MAMMADOVA1, Ikram RUSTAMOV2, Narinj RUSTAMOVA3,
Gular IBRAHIMOVA1, Mehraj ABBASOV1, 4, Sevda BABAYEVA1*
1Genetic Resources Institute, Ministry of Science and Education of the Republic of Azerbaijan, AZ1106, Baku, Azerbaijan
2 Nefes psychoneurological center, Baku, Azerbaijan
3 Narinj Psychology Center, Baku, Azerbaijan
4Western Caspian University, AZE1072, Baku Azerbaijan
Abstract
Mammadova Z., I.Rustamov, N. Rustamova, G. Ibrahimova, M. Abbasov, S. Babayeva (2024). Study of neuropilin-2 rs849563 gene polymorphism in children with autism spectrum disorder. - Genetika, Vol 56, No.2, 337-345.
Autism Spectrum Disorder (ASD) is a significant developmental condition in children, resulting from a combination of genetic and environmental factors. This study focused on analyzing the NRP2 (rs849563) gene variation and its association to autism risk in a group of 36 Azerbaijani children, with 18 having ASD and 18 as controls. The genotyping of the single nucleotide polymorphism (SNP) was carried out using PCR-RFLP analyses. The results of the analysis showed that in autistic children, 61.1% had the TT genotype, and 38.9% had the TG genotype, while in the control group, the frequencies were 55.6% for TT and 44.4% for TG. The GG genotype was not found in either group. Statistical analysis revealed no clear link between genotypes and the likelihood of developing autism (OR = 0.7955, 95% CI = 0.21 - 3.00, P = 0.7355). The T and G allele frequencies were 80.6% and 19.4% in the ASD group, and 77.8% and 22.2% in the control group, respectively. The study suggested that the NRP2 (rs849563) T allele might be associated with a higher risk of autism compared to the G allele, according to binary logistic regression analysis (OR = 1.18, 95% CI = 0.38 to 3.7). However, this association did not reach statistical significance (P = 0.7718). Additionally, when comparing the genotypes of parents and autistic children, a transmission disequilibrium analysis showed no significant imbalance for the rs849563 marker (LRS=1.14, df=1, P=0.29).
Keywords: ASD, gene, polymorphism, NRP2, PCR-RFLP
Corresponding author: Sevda Babayeva, Genetic Resources Institute of Ministry of Science and Education of Azerbaijan, Azadlig Ave 155, AZ1106, Baku, Azerbaijan, e-mail: seva_genetic@yahoo.com, phone: +994505327819
Published in „GENETIKA“ Vol. 56, No. 2(2024), pp. 347-356
© 2024 Serbian Genetics Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/ GENSR2402347K
Original
scientific article
NEW VARIETY OF WHITE LUPINE MONICA (Lupinus albus L.)
Valentin KOSEV1, Viliana VASILEVA2, Vera POPOVIÆ3
1Institute of Forage Crops, 5800 Pleven, Agricultural Academy, Bulgaria
2Maize Research Institute, 5835 Knezha, Agricultural Academy, Bulgaria
3Institute of Field and Vegetable Crops, 21000 Novi Sad, R. of Serbia
Abstract
Kosev V., V. Vasileva, V. Popoviæ (2024). New variety of white lupine Monica (Lupinus albus L.). - Genetika, Vol 56, No.2, 347-356.
Characterization of promising white lupine hybrid lines (LN5 and LN1-1) and their parental forms (Lucky801 and PI533704) was done. Biometric analysis of each variety and line was performed according to main quantitative traits and phenological stages and periods. A new variety of white lupine Monica (from line LN5) was created with a direction of use for seeds. It was obtained through repeated individual selection from the cross Lucky801 × PI533704. The variety belongs to the medium-ripening group with a duration of the vegetation period of 129 days. It has genetically completed growth. The height of the plant was 87 cm. The variety has high lodging resistance and good adaptability to abiotic conditions. The first pods were formed at 40 cm. The pods of the plant are formed on the main stem and on shortened lateral branches of the first and second order. About 28 uncrackable pods formed on one plant. The inflorescence is medium-sized, blue-white, the seeds are white, medium-sized. The number of seeds in one plant is on average 114. The protein content of the grain is 33%. The variety is distinguished by high potential seed productivity (the mass of seeds per plant 37 g). The mass of 1000 seeds is 339 g. The average yield of grain (3150 kg/ha) in the competition variety trials exceeds the average yield of the parental forms (2940 kg/ha). By decision of the Expert Commission at the Executive Agency for Variety Testing, Field Inspection and Seed Control, Bulgaria (appointed by order RD-10-1/14.01.2022) and by order No. RD-12-1 of the Minister of Agriculture of the Republic of Bulgaria dated 02.03.2022, the Monica variety was recognized as a new and original.
Keywords: white lupine, productivity, yield, variety
Corresponding author: Viliana Vasileva, Maize Research Institute, 5835 Knezha, Agricultural Academy, Bulgaria, e mail: viliana.vasileva@gmail.com
Published in „GENETIKA“ Vol. 56, No.2 (2024), pp. 357-368
© 2024 Serbian Genetics
Society
S. Bajiæa 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2402357K
Original
scientific article
GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP
IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
Alexander KRIVORUCHKO1,2,*, Andrey LIKHOVID2, Anastasiya KANIBOLOTSKAYA1, Olga KRIVORUCHKO1, Larisa SKORYKH1, Natalia KIZILOVA3, Olesya YATSYK1
1FSBSI "North Caucasian Federal Scientific Agrarian Center", 356241
Mikhailovsk, Russian Federation
2FSAEI HE "North Caucasian Federal University", 355017, Stavropol, Russian Federation
3Stavropol State Agrarian University, 355017, Stavropol, Russian Federation
Abstract
Krivoruchko A., A. Likhovid, A. Kanibolotskaya, O. Krivoruchko, L. Skorykh, N. Kizilova, O. Yatsyk (2024). Genome-wide search polymorphisms using Illumina beadchip in Russian meat Merino sheep for future genotyping by sequencing. - Genetika, Vol 56, No.2, 357-368.
For the mass use of genotyping by sequencing in sheep of the Russian Meat Merino breed, it is necessary to determine the loci of the genome with a sufficient frequency of occurrence in the population. To identify them, genotyping of Russian Meat Merino sheep was carried out using Ovine Infinium HD BeadChip 600K. As a result of polymorphism evaluation of 606,000 loci, 555 SNPs were selected with a frequency of occurrence of both homozygous variants in the range of 0.2850-0.3149. After excluding substitutions located closer than 1cM, a list of 387 polymorphisms was obtained. The selected substitutions were located on all 26 autosomes. The greatest number of polymorphisms were on the 1, 3, 6, 9, 12 and 22 chromosomes. The least substitutions were found on chromosomes 4, 8, 11 and 19. Only one substitution with the required frequency of occurrence was identified on the X chromosome. The average distance between SNPs was 4,000 to 7,000 kbp. The list of polymorphisms we have chosen can be used to confirm the reliability of the origin in the molecular genetic examination of sheep of the Russian Meat Merino breed.
Keywords: sheep, SNP, genotyping by sequencing, NGS, breeding, Russian Meat merino
Corresponding author: A. Krivoruchko, FSBSI "North Caucasian Federal Scientific Agrarian Center", 356241 Mikhailovsk and FSAEI HE "North Caucasian Federal University", 355017, Stavropol, Russian Federation,E-mail: rcvm@yandex.ru