GENETIKA, Vol. 56, No.3 (2024)
Tayyaba SHAN, Sabika FIRASAT, Bushra GUL, Rizwan ALI, Kiran AFSHAN
CDKAL1 rs10946398 POLYMORPHISM AS A CRITICAL GENETIC DETERMINANT FOR GESTATIONAL DIABETES MELLITUS IN PAKISTANI PREGNANT WOMEN [Abstract] [Full text]
Alireza TARINEJAD, Mostafa ALAMHOLO and Khadijeh DEL AZAD
A STUDY ON CHICKPEA (Cicer arietinum
L.) EST UNDER SALINITY STRESS AND DEVELOPMENT OF EST-SSRS MOLECULAR MARKERS
Remzi ÖZKAN
WATER USE AND YIELD PERFORMANCE OF DURUM WHEAT GENOTYPES UNDER SUPPLEMENTAL IRRIGATION AND RAINFED-BASED GROWTH CONDITIONS [Abstract] [Full text]
Marija KOSTADINOVIĆ, Danijela RISTIĆ,
Sofija BOŽINOVIĆ, Vlatko GALIĆ, Dragana IGNJATOVIĆ-MICIĆ, Jelena VANČETOVIĆ
MARKER ASSISTED SELECTION OF PARENTAL
MAIZE INBRED LINES FOR PYRAMIDING OF OPAQUE2 AND CRTRB1
GENES [Abstract]
[Full
text]
Debi Rani DATTA, Mohd Y. RAFII, Azizah MISRAN, Mashitah JUSOH, Oladosu YUSUFF, Md. Shalim UDDIN
ESTIMATION OF GENETIC ANALYSIS BY USING HALF DIALLEL CROSSES UNDERLYING EGGPLANT FOR TROPICAL CLIMATES [Abstract] [Full text]
Merve BAYHAN
ASSESSMENT OF
CROP YIELD AND CHARACTERISTICS IN DIFFERENT BREAD WHEAT (Triticum aestivum
L.) UNDER RAINFED AND IRRIGATED ENVIRONMENTS FOR
POTENTIAL FOOD INDUSTRY APPLICATIONS [Abstract] [Full
text]
Danka MILOVANOVIĆ, Valentina NIKOLIĆ*, Beka
SARIĆ, Marijana SIMIĆ, Slađana ŽILIĆ
COMPARISON OF WET-MILLING PROPERTIES AND STARCH
CHARACTERIZATION OF TEN MAIZE GENOTYPES FOR POTENTIAL FOOD INDUSTRY
APPLICATIONS [Abstract] [Full
text]
Deepak KALE, Jaya SINGH, Pranjal KHANDARE, Yogesh SATHE and Dinesh PATIL
RELATIONSHIP BETWEEN POLYMORPHISM OF STAT1 AND PPARGC1A GENES AND MILK PRODUCTION TRAITS IN GAOLAO CATTLE IN LENTIL VARIETIES [Abstract] [Full text]
Abdullah EFE and Behiye Tuba BICER
IMPACT OF ETHYL METHANE SULFONATE (EMS) AND GAMMA RADIATION ON MUTAGENIC INDUCTION IN LENTIL VARIETIES [Abstract] [Full text]
Marina ABRAMOVA, Anna
ILINA, Evgeny EVDOKIMOV, Аlexander KONOVALOV
A COMPLEX ASSESSMENT OF THE NENETS REINDEER POPULATION
USING PHENOTYPE INDICATORS DEPENDING ON POLYMORPHISM OF MEAT PRODUCTIVITY GENES [Abstract] [Full
text]
Ana PEJČIĆ, Ivan MINIĆ, Milena KOSTIĆ, Marija BRADIĆ-VASIĆ, Džemil KURTAGIĆ
GENETIC PREDISPOSITION TO A PERIODONTAL DISEASE. A REVIEW [Abstract] [Full text]
Published in „GENETIKA“ Vol. 56, No3(2024), pp. 369-380
© 2024 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575.369
https://doi.org/10.2298/GENSR2403369S
Original
scientific article
CDKAL1 rs10946398 POLYMORPHISM AS A CRITICAL GENETIC DETERMINANT FOR GESTATIONAL DIABETES MELLITUS IN PAKISTANI PREGNANT WOMEN
Tayyaba SHAN1, Sabika FIRASAT1*, Bushra GUL1, Rizwan ALI2, Kiran AFSHAN1
1Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
2Department of Statistics, Virtual University of Pakistan
Abstract
Shan T., S. Firasat, B. Gul, R. Ali, K. Afshan (2024). CDKAL1 rs10946398 polymorphism as a critical genetic determinant for gestational diabetes mellitus in Pakistani pregnant women. - Genetika, Vol 56, No.3, 377-380.
The likelihood of developing Gestational Diabetes Mellitus (GDM) is influenced by interplay of genetic and non-genetic factors. Key non-genetic contributors include elevated blood sugar levels, obesity, the inter pregnancy interval, and advanced maternal age at conception. Additionally, polymorphic variations within the CDKAL1 gene have also been identified as contributing factors to GDM susceptibility in different ethnic populations. CDKAL1 (Cyclin-Dependent Kinase 5 Regulatory Subunit Associated Protein 1-Like 1) gene on chromosome 6p22.3, is responsible for encoding a protein of methylthiotransferase family. This protein is involved in critical biological pathways related to tRNA processing and intron-containing pre-mRNA processing. GDM, a complex disorder characterized by reduced insulin sensitivity, affects glucose regulation and increases the risk of severe long-term complications, including type 2 diabetes mellitus (T2DM) and damage to organs like the kidneys, eyes, blood vessels, and nerves. Single nucleotide polymorphisms (SNPs) found within an intronic segment of the CDKAL1 have been shown to increase vulnerability to both GDM and T2DM. Current case-control study conducted at Wah General Hospital of Punjab Province, Pakistan enrolled 78 pregnant women diagnosed with GDM and 78 non-diabetic pregnant women who underwent prenatal assessments. Statistical analysis using SPSS 21.0 was performed to check the association of risk factors associated with GDM. The rs10946398 variant in the CDKAL1 gene was genotyped using the Tetra ARMS-PCR (Amplification Refractory Mutation System). The analysis revealed a significant association of GDM with different non-genetic risk factors. We also identified a positive association of the CDKAL1 SNP rs10946398 and an elevated risk of gestational diabetes (p<0.05) among the study participants. These findings provide additional evidence supporting the association of the rs10946398 variant's susceptibility to gestational diabetes in Pakistani pregnant women. Nonetheless, further extensive large-scale analyses are required within Pakistani population to elucidate the potential involvement of other genetic variants associated with the disease.
Keywords: Gestational diabetes mellitus, Pakistani population, ARMS PCR, rs10946398, CDKAL1
Corresponding author: Sabika Firasat, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, University Road, 45320, Islamabad Pakistan, phone: 00925190644410, E-mail: sabika.firasat@qau.edu.pk
Published in „GENETIKA“ Vol. 56, No. 3 (2024), pp.381-399
© 2024 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630.381
https://doi.org/10.2298/GENSR2403381T
Original scientific article
A STUDY ON CHICKPEA (Cicer arietinum L.) EST UNDER SALINITY STRESS
AND DEVELOPMENT OF EST-SSRS MOLECULAR MARKERS
Alireza TARINEJAD 1, 2*, Mostafa ALAMHOLO 3 and Khadijeh DEL AZAD1
1Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
2 The Halophyte Biotechnology Research Center, Azarbaijan Shahid Madani University, Tabriz-Iran
3Department of Biotechnology, Institute of Science and Modern Technology, Rojava University, Qamishlo, Syria
Abstract
Tarinejad A., M. Alamholo and K. Del Azad (2024). A study on chickpea (Cicer arietinum L.) EST under salinity stress and development of EST-SSRs molecular markers. - Genetika, Vol 56, No.3, 389-399.
Chickpea (Cicer arietinum L.) is one of the
most important staple food legume crops worldwide. For investigating molecular
marker development and genetic diversity, expressed sequence tags-simple
sequence repeats (EST-SSRs) are of particular importance. However, the number of key genes and molecular markers are
limited to undertake molecular breeding in chickpea and deal with abiotic
stresses. After downloading the EST
sequences from the NCBI website, we determined the contigs and singletons by
EGassembler software and the SSR in the EST sequences by SSRLocator software.
Therefore, 993 contigs and 1953 singletons were obtained under salinity stress.
Accordingly, 16 SSR markers could be introduced for selection of
tolerant cultivars to salt stress in chickpea. Moreover, contig294, contig307, and contig982; and singletons including
GR403187.1, GR408755.1, and GR403067.1 could be suggested as new genes in
controlling the salt stress tolerance mechanism containing SSR markers. The highest
percentage of TF in contigs and singletons was associated with AP2, ERF,
bZIP, Dof and WRKY. Moreover, the hub genes detected contained SSR marker
related to ABC transporter E family and 1-aminocyclopropane-1-carboxylate oxidase. To
evaluate the valid of this research, for eight of the contig, specific primers
were designed and the result was confirmed by five of them via PCR. Chickpea
ESTs generated sets are considered as high-quality transcripts for functional
markers development and gene discovery related to abiotic stress tolerance
which are able to facilitate chickpea breeding.
Keywords: Contigs, GR408755.1 Chickpea, EST-SSRs, Hub gene, Singletons
Published in „GENETIKA“ Vol. 56, No.3 (2024), pp.401-414
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630.11
https://doi.org/10.2298/GENSR240312002O
Original scientific article
WATER USE AND YIELD PERFORMANCE OF DURUM WHEAT GENOTYPES UNDER SUPPLEMENTAL IRRIGATION AND RAINFED-BASED GROWTH CONDITIONS
Remzi ÖZKAN
Department of Field Crops, Faculty of Agriculture, Dicle University, 21280, Diyarbakir,
Türkiye
Abstract
Özkan R.(2024). Water use and yıeld performance of durum wheat genotypes under supplemental ırrıgatıon and raınfed-based growth condıtıons. - Genetika, Vol 56, No.3, 401-414.
This study investigated the effects of supplemental irrigation on durum wheat genotypes during 2017-2018 and 2019-2020 at the Dicle University Faculty of Agriculture, Diyarbakir, Türkiye. Dry conditions in 2017 and 2018, with low soil moisture and high temperatures during the heading stage, increased water demand during the generative period. However, in 2019-2020 there was higher rainfall than usual. The analysis revealed significant genotypic variability, particularly during the first year. Supplemental irrigation had a positive effect on plant height, leaf area index, normalized difference vegetation index, chlorophyll content, grain yield, test weight and kernel weight in both the years. The leaf area index, normalized difference vegetation index and chlorophyll content increased significantly with supplemental irrigation, especially during 2019-2020. Grain yield had a positive response in both years, with Cesare showing the highest increase (211.2%) in the first year and TBT16-7 showing the highest increase (39.4%) in the second year. The water use efficiency values followed this positive trend, with Hat-300 showing the highest values under both rainfed and irrigated conditions. Strong positive correlations were found between grain yield and various traits, providing insights into the adaptability and performance dynamics of durum wheat genotypes under different water conditions, which is essential for optimizing wheat production in arid regions. The Cesare, TBT16-7, and Frat-93 genotypes showed greater promise for the region than did the other varieties. The use of these varieties in agriculture should be expanded to increase overall production.
Keywords: Durum wheat, ırrigated, rainfed, yield, water use efficiency
Corresponding author: Remzi Özkan, Department of Field Crops, Faculty of Agriculture, Dicle University, 21280, Diyarbakir,Türkiye,e-mail address: rmziozkan@gmail.com,Mobile phone: +90 542 843 37 87
Published in „GENETIKA“ Vol. 56, No3(2024), pp. 415-422
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630.15.3
https://doi.org/10.2298/GENSR2403415K
Original scientific article
MARKER ASSISTED SELECTION OF PARENTAL MAIZE INBRED LINES
FOR PYRAMIDING OF OPAQUE2 AND CRTRB1 GENES
Marija KOSTADINOVIĆ1*, Danijela RISTIĆ1, Sofija BOŽINOVIĆ1, Vlatko GALIĆ2,
Dragana IGNJATOVIĆ-MICIĆ1, Jelena VANČETOVIĆ1
1Maize Research Institute Zemun Polje, Belgrade, Serbia
2Agricultural Institute Osijek, Osijek, Croatia
Abstract
Kostadinović M., D. Ristić, S. Božinović, V. Galić, D. Ignjatović-Micić, J. Vančetović (2024). Marker assisted selection of parental maize inbred lines for pyramiding of OPAQUE2 and CRTRB1 genes. - Genetika, Vol 56, No.3, 415-422.
Marker assisted selection (MAS) is used in breeding to select progenies with the desired genes. MAS based gene pyramiding could facilitate in pyramiding of multiple genes for the simultaneous expression of more than one gene in a single genetic background. The main advantages of this strategy for germplasm improvement are elimination of extensive phenotyping, controlling the linkage drag and reducing the breeding duration. The aim of this study was to identify maize inbred lines that could serve as donors of opaque2 and crtRB1 favorable alleles. These genes can work together in the same genetic background to control the content of lysine, tryptophan, and beta-carotene. Three SSR markers, phi057, phi112 and umc1066, linked to the opaque2 gene, and crtRB1-3′TE, linked to the crtRB1, were employed for parental polymorphism screening. Recurrent parent (RP) was clearly distinguishable with crtRB1-specific marker from the donor parent (DP). The donors carried the favorable allele of crtRB1 (543 bp), while the recurrent parent exhibited the unfavorable crtRB1 allele (296 bp). Out of six DP lines, only one (DP1) was distinguishable with opaque2-specific markers from the RP line. The phi057 amplified 170 bp fragment in DP and 160 bp fragment in RP, while umc1066 amplified 150 bp fragment in DP and 160-170 bp fragment in RP. The phi112 showed a null fragment with DP and a band size around 170bp with RP. Therefore, DP1 was identified as the inbred line with potential to be used as a donor for pyramiding of opaque2 and crtRB1 genes, producing the breeding materials with high contents of lysine, tryptophan and beta-carotene, the major nutritional traits in maize.
Keywords: maize, marker assisted gene pyramiding, lysine, tryptophan, beta-carotene
Corresponding author: Marija Kostadinović, Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia, Phone: 011 3756704, Fax: 011 3756707, e-mail: kmarija@mrizp.rs
Published in „GENETIKA“ Vol. 56, No.3 (2024), pp. 423-442
© 2024Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2403423D
Original
scientific article
ESTIMATION OF GENETIC ANALYSIS BY USING HALF DIALLEL CROSSES UNDERLYING EGGPLANT FOR TROPICAL CLIMATES
Debi Rani DATTA1,3*, Mohd Y. RAFII 1,2, Azizah MISRAN2, Mashitah JUSOH2,
Oladosu YUSUFF 1, Md. Shalim UDDIN3
1Laboratory of Climate-smart Food Crop Production, Institute of Tropical Agriculture and Food Security, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
2Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Serdang 43400, Selangor, Malaysia
3Oilseed Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh
Abstract
Datta R. D., M.Y. Rafii, A. Misran, M., O. Yusuff, Md. S. Uddin (2024). Estimation of genetic analysis by using half diallel crosses underlying eggplant for tropical climates. - Genetika, Vol 56, No.3, 423-442.
Predicting and evaluating hybrid performance is essential in eggplant (Solanum melongena) breeding. Based on this background, 55 F1 hybrids derived from 11 × 11 half-diallel fashion were evaluated on agronomic and morphological conventional descriptors in two locations. Different genetic components of variation were also calculated for all the studied characters. Almost all the characters showed significant environmental effects except the number of main stems per plant and fruit production per plant. The value of mean squares for GCA was larger than those values of SCA for all variables except fruit production per plant, demonstrating that the additive gene action predominated for both locations. Additionally, Baker’s ratio for the characters under investigation ranged from 0.66 to 0.98, showing that the additive effect is prevalent among the evaluated characters. The dominant genetic effects (b) were smaller than additive genetic effects (a) of all the studied characters except for fruit production per plant. Positive dominance (H1 and H2) values were obtained for all the studied characters, proving the significance of non-additive components in the inheritance of these characters. Moreover, the value of h2/H2 was less than one for all the studied characters revealing that one major gene group was involved for all characters. Therefore, it was indicated that hybrid breeding might be effectively employed to increase production and improve quality attributes in eggplant.
Keywords: Eggplant, Genetic analysis, Half diallel, Hayman and Jinks method, Vr-Wr graph
Corresponding author: Debi Rani Datta, Laboratory of Climate-smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia, Oilseed Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh, phone: +8801717559076, E-mail: debi.datta@yahoo.com
Published in „GENETIKA“ Vol. 56, No. 3(2024), pp. 443-458
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630.11
https://doi.org/10.2298/GENSR240312001B
Original
scientific article
ASSESSMENT OF CROP YIELD AND CHARACTERISTICS IN DIFFERENT BREAD WHEAT (Triticum aestivum L.) UNDER RAINFED AND IRRIGATED ENVIRONMENTS
Merve BAYHAN
Department of Field Crops, Faculty of Agriculture, Dicle University, 21280, Diyarbakir,
Türkiye
Abstract
Bayhan M. (2024). Assessment of crop yıeld and characterıstıcs ın dıfferent bread wheat (Triticum aestivum L.) under raınfed and ırrıgated envıronments. - Genetika, Vol 56, No.3, 443-458.
Achieving the goal of increasing both crop yield and water use efficiency through better precipitation regimes is a major challenge in semi-arid and arid regions. The objective of this study was to assess the performance and agronomic traits and water use efficiency (WUE) of bread wheat genotypes under rainfed and irrigated conditions. This study was conducted in the Research and Application Field of the Faculty of Agriculture at Dicle University in Diyarbakır, Türkiye. Nine wheat genotypes were used as the experimental materials. Notably, the first year of the study exhibited decreased values for most traits under rainfed conditions compared with irrigated conditions, a trend that was less pronounced in the second year and varied based on climatic conditions. Noteworthy findings include the high performance of the Dinç and Wafia varieties, which displayed significant improvements in grain yield with supplemental irrigation. Heading time, normalized difference vegetation index, leaf area index, plant height, and thousand-kernel weight were identified as crucial factors influencing grain yield. In the first year, the DZ17-1 and Empire genotypes and in the second year, the DZ17-1, Ceyhan-99, and Dinç genotypes demonstrated the highest grain yield potential. Notably, Empire variety exhibited superior performance under dry conditions, underscoring its drought resilience. This study found a linear relationship between grain yield and water use efficiency under rainfed and irrigated conditions, with DZ17-1 achieving the highest yield. These findings highlight the importance of selecting the appropriate wheat genotypes and irrigation strategies to mitigate the effects of drought on wheat production.
Keywords: Drought, wheat, water use efficiency, supplemental irrigation, climate change
Corresponding author: Merve Bayhan, Department of Field Crops, Faculty of Agriculture, Dicle University, 21280, Diyarbakir, Turkiye, e-mail address: mervebayhan21@gmail.com, Mobile phone: +90 546 766 76 09
Published in „GENETIKA“ Vol. 56, No3(2024), pp 459-474
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.15
https://doi.org/10.2298/GENSR2403459M
Original
scientific article
COMPARISON OF WET-MILLING PROPERTIES AND STARCH CHARACTERIZATION OF TEN MAIZE GENOTYPES FOR POTENTIAL FOOD INDUSTRY APPLICATIONS
Danka MILOVANOVIĆ, Valentina NIKOLIĆ*, Beka SARIĆ, Marijana SIMIĆ, Slađana ŽILIĆ
Maize Research Institute, Zemun Polje, Department of Food Technology and Biochemistry,
Belgrade-Zemun, Serbia
Abstract
Milovanović D. V. Nikolić, B. Sarić, M. Simić, S. Žilić (2024). Comparison of wet-milling properties and starch characterization of ten maize genotypes for potential food industry applications. - Genetika, Vol 56, No.3, 459-474.
Starch is the primary component of the maize grain which accumulates in the endosperm and accounts for approximately 70% of the dry matter. The starch of ten selected maize genotypes (six yellow, three waxy yellow, and one red genotype) was characterized in this study in order to determine the possibility of application in the food industry. The yield of starch and by-products (maize gluten, germ, brain), percentage of starch recovery, as well as in vitro digestibility of starch and its structural and gelling properties were determined after conducting a laboratory wet milling process. The percentage of starch recovery ranged from 70.31% (ZP 6073wx) to 90.84% (ZP 299). The starch of seven standard grain yellow genotypes had the normal amylose content (20-24%), while the three waxy genotypes had the expected amylopectin content of 100%. The amylopectin starch of genotype ZP 6066wx had the highest swelling power (SP) (13.12 g/g) and the highest solubility index (WSI) (20 g/100g), while the genotype with standard red grain had the highest level of water absorption index (WAI) (12.75 g/g). The results showed that the starch obtained from the investigated maize genotypes meets the food grade quality criteria. Therefore, it is suitable for a wide range of applications in the food industry.
Keywords: maize starch, genotypes, wet milling, in vitro digestibility, gelling properties
Corresponding author Valentina Nikolić, Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade-Zemun, phone: +381 648406448; e-mail: valentinas@mrizp.rs
Published in „GENETIKA“ Vol. 56, No.3 (2024), pp.475-487
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2403475K
Original
scientific article
Deepak KALE*, Jaya SINGH, Pranjal KHANDARE, Yogesh SATHE and Dinesh PATIL
Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU),
Nagpur-440006, Maharashtra, India
Abstract
Kale D., J. Singh, P. Khandare, Y. Sathe and D. Patil (2024). Relationship between polymorphism of STAT1 and PPARGC1A genes and milk production traits in Gaolao cattle. - Genetika, Vol 56, No.3, 475-487.
Current dairy genomics research emphasizes on identification of polymorphisms within candidate genes and their association with economic traits. The current study was planned to detect polymorphisms within 3’UTR region of STAT1 gene and intron-9, 3’UTR and exon-24 regions of PPARGC1A candidate gene in Gaolao cattle population. The study included 220 to 286 unrelated animals of true to type Gaolao breed. The genomic DNA was isolated and the test day traits were recorded. The gene polymorphisms were explored using PCR-RFLP, PCR-SSCP & Direct DNA sequencing tools. The PCR-RFLP screening in 3’UTR region of STAT1 gene revealed polymorphism at STAT1G1 -PagI loci with frequency for A allele as 0.1573 and frequency for C allele as 0.8427 at the locus. The association study revealed significant difference in Fat % (5.16±0.83*) and Protein % (3.23±0.04*) milk production trait for ‘AC’ genotype as compared to CC genotypes. The screening in intron-9 region of PPARGC1A gene at PPARGCA1G2-HaeIII locus revealed frequency for the A alleles as 0.5116, and for the allele B as 0.4884. The association analysis reveled non-significant differences between genotypes on milk yield and components. PPARGC1AG5-SSCP within 414 bp fragment of exon-24 region revealed polymorphism with frequency of pattern A=0.25 and Pattern B=0.75 in 56 samples of Gaolao cattle. Direct sequencing of pattern A and B confirmed presence of SNP G-A at 194th position in 325 bp sequence. Results of the current study indicate existence of variation within different regions of functionally important STAT1 and PPARGC1A candidate genes. The identified polymorphisms and association within STAT1 and PPARGC1A candidate genes can be useful in selection and breeding for Gaolao cattle breed improvement.
Keywords: gene polymorphisms, milk yield, PPARGC1A, STAT1, Zebu cattle
Corresponding author: Dr. Deepak Kale, Assistant Professor and Head, Department of Animal Genetics & Breeding, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Seminary Hills, Nagpur-440006, Maharashtra State, India. E-mail: deepakkaleccmb@gmail.com, Phone: 91-9604747652
Published in „GENETIKA“ Vol. 56, No. 3(2024), pp. 489-502
© 2024 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2403489E
Original
scientific article
IMPACT OF ETHYL METHANE SULFONATE (EMS) AND GAMMA RADIATION
ON MUTAGENIC INDUCTION IN LENTIL VARIETIES
Abdullah EFE1* and Behiye Tuba BICER2
1GAP International Agricultural Research and Training Center, Department of Field Crops, Diyarbakir, Türkiye
2 Dicle University, Faculty of Agriculture, Department of Field Crops, Diyarbakir, Türkiye
Abstract
Efe A. and B. T. Bicer (2024). Impact of ethyl methane sulfonate (EMS) and gamma radiation on mutagenic induction in lentil varieties. - Genetika, Vol 56, No.3, 497-502.
The disappearance of local varieties and the fact that introduction varieties show almost the same genetic characteristics have begun to reveal limitations in lentil cultivation in the Southeastern Anatolia of Türkiye, where this study was conducted and which is also an important origin center. Mutation studies are used to reveal different genetic characteristics in lentils, which have a narrow genetic base. In this research, four lentil varieties were treated with 100, 200 and 300 Gy of gamma rays and 20, 40, 60, 80 and 100 mM of Ethyl methanesulfonate (EMS). Effect of gamma doses and EMS concentrations on germination rate index, mean germination time and abnormal germination among varieties were different, and high doses generally delayed germination parameters. Seedling and root traits were reduced by high EMS concentrations, while gamma doses showed inconsistent differences among cultivars. LD50 for gamma irradiation was found to be 266.68 Gy in Koc-21 variety, which represents the lowest value among the studied varieties. GR50 indicated that EMS concentrations were suitable for generating mutants. GR50 values for gamma treatments could not be determined at the doses and varieties used and high gamma doses were necessary to generate effective mutant populations. EMS treatments showed significant mutagenic effects with a dose of 40 mM showing significant mutagenic effects in field experiments. The most effective dose was 80 mM, with higher doses (100 mM) leading to increased corrected mortality but not proportionally higher mutagenic efficiency.
Keywords: Lentil, Lens culinaris, Mutation, Gamma, Ethyl methanesulfonate
Corresponding author: Abdullah EFE, GAP International Agricultural Research and Training Center, Department of Field Crops, Diyarbakir, Türkiye, E-mail: abdullah.efe@tarimorman.gov.tr, phone:+90534283422
Published in „GENETIKA“ Vol. 56, No.3 (2024), pp. 503-516
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.503
https://doi.org/10.2298/GENSR2403503A
Original scientific article
A COMPLEX ASSESSMENT OF THE NENETS REINDEER POPULATION USING PHENOTYPE INDICATORS DEPENDING ON POLYMORPHISM OF MEAT PRODUCTIVITY GENES
Marina ABRAMOVA 1*, Anna ILINA 1.2, Evgeny EVDOKIMOV 1,2,
Аlexander KONOVALOV1
1Federal Williams Research Center of Forage Production & Agroecology, Lobnya, Russia,
2 P.G. Demidov Yaroslavl State University, Yaroslavl, Russia
Abstract
Abramova M., A. Ilina, E. Evdokimov, А. Konovalov (2024). A complex assessment of the Nenets reindeer population using phenotype indicators depending on polymorphism of meat productivity genes. - Genetika, Vol 56, No.3, 511-516.
The aim of the study was to study the genetic structure of the reindeer population of the native Nenets breed and to evaluate the morphometric parameters in the resulting genetic groups. Pearson's Chi-square (χ2) test was used to assess the statistical significance of differences between the empirical and theoretical genotype frequencies. Theoretical occurrence frequencies were calculated based on the Hardy-Weinberg law. A z-test was used to identify partial differences between the empirical and theoretical distributions. The software Python 3.9.7, GenAlEx 6.51 was used for data processing and statistical analysis. The population studied according to STR profiles is split into three independent groups (I, II, III) with sizes of 33, 53, 16 individuals, respectively. Genetic differentiation between the identified subpopulations was weak, but statistically significant (Fst = 0.022, p = 0.01). The greatest differentiation is observed between subpopulations I and III (Fst = 0.025, p = 0.03), while the differences between I - II and II - III are less pronounced (Fst = 0.017, Fst = 0.015). In subpopulations I and II, all loci are polymorphic; in subpopulation III, only 93.75% of the loci are polymorphic. The average number of alleles per locus is 6.44±0.63, 6.56±0.60, 5.25±0.54, respectively, for subpopulations I, II, III. The effective number of alleles for subpopulations is 3.73±0.44, 3.55±0.38, 3.40±0.40, respectively. The Shannon information index for the three subpopulations is at the same level and amounts to 1.38±0.13, 1.36±0.12, 1.27±0.13. The distribution according to the Hardy-Weinberg law and the empirical distribution of heads according to the genotypes of the growth hormone gene AA, AB and BB are statistically significantly different and are 36.603:47.795:15.603 and 22:77:1, respectively. Therefore, this equilibrium is absent in the studied population and a shift in the distribution of allele frequencies is observed. Genetic drift, migration processes or selection pressure may mediate such a shift. For the myostatin gene, no statistically significant differences were found between the theoretically expected and empirical distributions of genotypes. Females with the GHAA genotype had a higher live weight than their peers with the GHAB genotype. The study of reindeer exterior measurements and physique indices in conjunction with somatotropin gene polymorphism found that individuals with the GHAA genotype outperform their peers with the GHAB genotype, while low variability of indicators for these traits was observed. In the studied sample of both sex and age groups, a rather high value of the Gregory index (0.86...0.99) was found, which is typical for beef cattle. The obtained results can serve as the basis for the development of a system of genetic assessment in reindeer breeding and indicate the priority groups of animals for further breeding.
Keywords: Rangifer tarandus. STR, GH, MSTN, body type, live weight.
Corresponding author: Marina Abramova, Federal Williams Research Center of Forage Production & Agroecology, Lobnya, Russia, E-mail: abramovam2016@yandex.ru
Published in „GENETIKA“ Vol. 56, No. 3(2024), pp. 517-534
© 2024 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2403517P
Review article
GENETIC PREDISPOSITION TO A PERIODONTAL DISEASE REVIEW
Ana PEJČIĆ1*, Ivan MINIĆ1,2,
Milena KOSTIĆ3, Marija BRADIĆ-VASIĆ1,2,
Džemil KURTAGIĆ1,2
1Department of Periodontology and oral medicine, Medical faculty, University of Nis, Nis, Serbia
2Postdoctoral Study, Department of Periodontology and oral medicine, Medical faculty, University of Nis, Nis, Serbia
3Department of Prosthodontics, Medical faculty, University of Nis, Serbia, Serbia
Abstract
Pejčić A., I. Minić, M. Kostić, M. Bradić-Vasić, Dž. Kurtagić (2024). Genetic predisposition to a periodontal disease review. - Genetika, Vol 56, No.3, 525-534.
Periodontal disease is a complex, multifactorial disease and the predisposition to its occurrence is genetically determined. A multitude of research projects on genotyping of patients and controls in search of putative genetic risk factors has been performed. The disease however is also dependent on the presence of environmental and lifestyle factors, and their interaction with the various genes. Many candidate genes have been proposed and studied in relation to periodontitis. Most of the studied candidate genes code for proteins that play a role in the innate immune system. Both, IL-1 and TNF-a play an important role in the development of periodontal disease. Till today there is no strong evidence for target genes and gene polymorphisms that play a key role in the susceptibility to and severity of periodontal disease.
Keywords: cytokines, gene, periodontal disease, predisposition