GENETIKA, Vol

GENETIKA, Vol. 57, No1 (2025)

Solomon AKINYOSOYE, Morufat BALOGUN, Samuel OLAKOJO, Johnson ADETUMBI

GENE ACTION AND HETEROSIS FOR AGRO-NUTRITIONAL QUALITY TRAITS OF MAIZE HYBRIDS IN HUMID AGRO-ECOLOGIES OF NIGERIA [Abstract] [Full text] [AKINYOSOYE data supplement]

Fariborz SHEKARI, Naser SABAGHNIA, Amin ABBASI, Ramin BALJANI

EVALUATION OF IMPORTANT TRAITS AFFECTING YIELD IN SAFFLOWER (Carthamus tinctorius L.) [Abstract] [Full text]

V.M. RASHMI, Parveen SHARMA, Manpreet KAUR, Akhilesh SHARMA, Nimit KUMAR, Aparajita DWIVEDI, Sanjay Kumar SANADYA

GENETIC DIVERSITY AND POPULATION STRUCTURE OF TOMATO (Solanum lycopersicum L.) GENOTYPES [Abstract] [Full text]

Faiza TAWAB, Adnan NASIM, Saleha TAWAB

GENETIC DIVERSITY ANALYSIS OF SELECTED LOCAL RICE VARIETIES OF PAKISTAN USING RAPD MARKERS [Abstract] [Full text]

Shruti KUMARI, S. K. CHAKRABARTY, Praloy KUMAR BHOWMICK, Paul DEBASHIS³
MOLECULAR PROFILING AND GENETIC DIVERSITY ASSESSMENT OF RICE (Oryza sativa L.) USING SSR MARKERS [Abstract] [Full text]

Sibel IPEKESEN and Behiye Tuba BICER

SHADING EFFECT ON PERFORMANCES OF CROP GROWTH AND YIELD PARAMETERS OF VINING PEA AT DIFFERENT DEVELOPMENTAL STAGES [Abstract] [Full text]

Natalia Anastasova GEORGIEVA, Valentin Ivanov KOSEV, Vera Miloševa POPOVIĆ

PROSPECTS FOR BREEDING AND HETEROSIS STUDIES FOR YIELD AND YIELD COMPONENTS IN WHITE LUPINE HYBRIDS (Lupinus. albus L.) [Abstract] [Full text]

Vineeta KAILA, S. S. KANDHOLA, Pankaj SHARMA

STABILITY AND GENOTYPE X ENVIRONMENT ANALYSIS FOR SEED AND OIL YIELD PARAMETERS AMONG INBRED LINES OF SUNFLOWER (Helianthus annuus L.) [Abstract] [Full text]

Lucia URBANOVÁ, Silvia FARKASOVÁ, Lenka KUČEROVÁ, Jana ŽIAROVSKÁ

Fingerprint and expression profiles of OARE – 1 retrotransposon in morphological parts of Avena sativa varieties [Abstract] [Full text]

Gurveer Kaur BRAR, Harmeet Singh SARALCH, Sapna THAKUR

GENETIC VARIABILITY AND PCA OF GROWTH AND PHYSIOLOGICAL TRAITS IN Ulmus villosa SEED SOURCES [Abstract] [Full text]

Iram SHEHZADI, Sabika FIRASAT, Kiran AFSHAN, Maryam ZUBAIR, Haiba KAUL

RISK FACTORS, CLINICAL ASPECTS, AND FOKI POLYMORPHISM OF VITAMIN D RECEPTOR GENE IN PREECLAMPSIA CASES ATTENDING COMBINED MILITARY HOSPITAL, MUZAFFARABAD, AZAD KASHMIR [Abstract] [Full text]

Published in „GENETIKA“ Vol. 57, No1(2025), pp. 1-22

UDC 575.633.15
https://doi.org/10.2298/GENSR240518001A
Original scientific article

GENE ACTION AND HETEROSIS FOR AGRO-NUTRITIONAL QUALITY TRAITS OF MAIZE HYBRIDS IN HUMID AGRO-ECOLOGIES OF NIGERIA

Solomon AKINYOSOYE^1,2*, Morufat BALOGUN¹, Samuel OLAKOJO²,

Johnson ADETUMBI²

¹Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria

²Institute of Agricultural Research and Training, Obafemi Awolowo University, P.M.B. 5029, Moor Plantation, Ibadan, Nigeria

Abstract

Akinyosoye S., M. Balogun, S. Olakojo, J. Adetumbi (2025). Gene action and heterosis for agro-nutritional quality traits of maize hybrids in humid agro-ecologies of Nigeria.- Genetika, Vol 57, No.1, 1-22.

Maize is a staple food of sub-Saharan African (SSA) populace but deficient in protein. Quality protein maize (QPM) has been developed to contain essential amino acids including lysine and tryptophan to alleviate malnutrition in SSA. This study was conducted to assess general and specific combining ability (GCA and SCA) effects, gene actions and heterosis among QPM hybrids for lysine and tryptophan at three locations in humid agro-ecologies of Nigeria. Grains from sib-mated full-sibs were analysed for lysine and tryptophan among ten inbreds, 45 hybrids generated by half-diallel and two checks. Results showed lysine and tryptophan contents ranged from 2.71% - 0.73% (Kishi) to 3.41% - 0.96% (Ibadan). Gene actions for expression of lysine and tryptophan varied with location; thus, additive gene action was preponderance in inheritance of lysine and tryptophan in Ibadan and Ile-Ife, whereas non-additive gene action was involved in inheritance of these traits in Kishi. Inbreds TZEEQI-8, TZEEQI3, TZEEQI-10, TZEEQI-12, TZEEQI-2 were best combiners for lysine and tryptophan. Hybrids TZEEQI-8×TZEEQI-16, TZEEQI-2×TZEEQI-12, TZEEQI 10×TZEEQI-7,TZEEQI-10×TZEEQI-12 and TZEEQI-8×TZEEQI-12 were adjudged as the top five hybrids for exhibiting positive and significant standard heterosis over commercial check with a range of 12.8%-16.7% for lysine and 15.4%-20.0% for tryptophan across locations. The relationship between grain yield with lysine and tryptophan was negative and significant across locations. Hence, breeding for high yielding QPM genotypes with high lysine and tryptophan contents at the same time, requires careful monitoring of lysine and tryptophan contents in the laboratory.

Keywords: Quality protein maize, lysine, tryptophan, grain yield, combining ability, heterosis

Corresponding author: Solomon Akinyosoye,Deparment of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria, phone:+2347039657868, e-mail: stakinyosoye@gmail.com

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Published in „GENETIKA“ Vol. 57, No. 1 (2025), pp.23-35

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2501023S
Original scientific article

EVALUATION OF IMPORTANT TRAITS AFFECTING YIELD IN SAFFLOWER (Carthamus tinctorius L.)

Fariborz SHEKARI, Naser SABAGHNIA*, Amin ABBASI, Ramin BALJANI

Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Iran

Abstract

Shekari F., N. Sabaghnia, A. Abbasi, R. Baljani (2025). Evaluation of important traits affecting yield in safflower (Carthamus tinctorius L.). - Genetika, Vol 57, No.1, 23-35.

The study assessed genetic diversity and trait relationships in 64 safflower genotypes obtained from the National Plant Gene-Bank of Iran. Employing an alpha lattice design with two replications, various agro-morphologic characteristics were measured. Analysis of pairwise association’s unveiled positively significant interrelationships of yield performance with the other measured characteristics, except for 1000-seed weight. This suggested that these traits generally moved in the same direction, positively influencing seed yield. To gain a more nuanced understanding of trait interdependencies, sequential path analysis was employed. This analysis identified the capitula of each plant and the seeds of each capitulum as crucial first-order characteristics significantly impacting seed yield. All direct effects identified through sequential path analysis were deemed significant, emphasizing the robustness of the findings. The analysis further categorized characteristics as second or third order variables according to impacts on seed yield. Leaf area, plant’s dry weight and 1000-seed weight were identified as second-order variables, indicating their indirect influence on seed yield. Additionally, height of plant, oil percentage, diameter of lateral capitulum and biological yield were categorized as third-order characteristics, further expanding the understanding of the complex trait relationships. The key takeaway from the study is the potential use of the capitula of each plant and the seeds of each capitulum as effective selection criteria for enhancing safflower yield performance. These findings provide valuable insights for safflower breeding programs, guiding the selection of genotypes with improved seed yield based on these identified influential traits.

Keywords: Multicollinearity, Path analysis, Resampling, Path analysis

Corresponding author: Naser Sabaghnia, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Iran, E-mail: sabaghnia@maragheh.ac.ir

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Published in „GENETIKA“ Vol. 57, No.1 (2025), pp.37-52

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2501037R

Original scientific article

GENETIC DIVERSITY AND POPULATION STRUCTURE OF TOMATO

(Solanum lycopersicum L.) GENOTYPES

V.M. RASHMI¹, Parveen SHARMA¹, Manpreet KAUR¹, Akhilesh SHARMA¹,

Nimit KUMAR¹, Aparajita DWIVEDI², Sanjay Kumar SANADYA^2*

¹Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Agricultural University, Palampur (Himachal Pradesh)-176062 India

²Department of Genetics and Plant Breeding, CSK Himachal Pradesh Agricultural University, Palampur (Himachal Pradesh)-176062 India

¹Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Agricultural University, Palampur (Himachal Pradesh)-176062 India

²Department of Genetics and Plant Breeding, CSK Himachal Pradesh Agricultural University, Palampur (Himachal Pradesh)-176062 India

Abstract

Rashmi V.M., P. Sharma, M. Kaur, A. Sharma, N. Kumar, A. Dwivedi, S. Kumar Sanadya (2025). Genetic diversity and population structure of tomato (Solanum lycopersicum L.) genotypes. - Genetika, Vol 57, No.1, 37-52.

In present study, morphological and molecular markers were used to assess the genetic diversity of 35 tomato genotypes. However, only 26 tomato genotypes were used to carry out clustering analysis due to Fusarium wilt disease nine tomato genotypes were died. Based on clustering analysis, tomato genotypes were grouped into twelve clusters whereas cluster III with eight genotypes followed by cluster I with seven genotypes. Maximum inter-cluster distance observed between cluster V and X indicating the existence of wide range of genetic diversity. Molecular diversity and population structure of 35 tomato genotypes were analyzed using polymorphic simple sequence repeat (SSR) markers. Total 74 alleles identified with an average of 2.05 alleles per locus, while the polymorphism information content of the primers ranged from 0.07 to 0.46 with a mean value of 0.32. The major allele frequency and heterozygosity ranged from 0.50-0.96 and 0.07-0.52, respectively. The dendrogram clustered genotypes into two main groups A and B with 27 and 8 genotypes, respectively but group A had two sub-clusters. Similarity coefficient ranged from 0.50 to 0.88 with maximum likeness between genotype BT-20-3 (Red egg shaped) & BT-20-3 (Yellow round) and NBHIA-5 & Punjab Upma while, minimum between Swarna Rattan & KSP-1154-5. Population structure revealed three sub-populations with some admixtures. Principal coordinate analysis revealed that the genotypes were uniformly distributed across the two axes in both the plots with 35.44 % of cumulative variation. An understanding of genetic diversity and polymorphism of tomato populations will help in exploiting the tomato genetic resources improvement.

Keywords: Genetic diversity, Polymorphism, Population structure, Tomato, Clustering.

Corresponding author: Sanjay Kumar Sanadya,Department of Genetics and Plant Breeding, CSK Himachal Pradesh Agricultural University, Palampur (Himachal Pradesh)-176062 India, Phone No.: +91 9460037450, E-mail: sanjaypbg94@gmail.com

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Published in „GENETIKA“ Vol. 57, No1(2025), pp. 53-62

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR2501053T

Original scientific article

GENETIC DIVERSITY ANALYSIS OF SELECTED LOCAL RICE VARIETIES

OF PAKISTAN USING RAPD MARKERS

Faiza TAWAB^1*, Adnan NASIM², Saleha TAWAB²

¹Department of Botany, Shaheed Benazir Bhutto Women University, Larama Peshawar, Pakistan

²Agriculture Research Khyber Pakhtunkhwa, Pakistan

Abstract

Tawab F., A. Nasim, S. Tawab (2025). Genetic diversity analysis of selected local rice varieties of Pakistan using RAPD markers. - Genetika, Vol 57, No.1, 53-62.

Assessment of the genetic diversity is very important for the improvement of many crop species including rice. The study was undertaken to assess the genetic diversity prevailing among ten commercially grown rice varieties of Pakistan using nine random amplified polymorphic DNA (RAPD) markers. All the nine primers were able to produce a total of 444 amplicons with an average of 49. 33 amplicon per primer. A total of 356 polymorphic amplicons were generated and showed 80.18 % polymorphism with an average number of 39.55 polymorphic bands per primer. RAPD marker, GL C-12 produced maximum number of bands (76 in all varieties) whereas GL G-14 generated minimum number of bands (26) in the genomic pool. A high level of genetic polymorphism at the DNA level was observed among the ten Oryza sativa varieties with an average genetic distance ranging from 28% to 64% on the basis of average dissimilarity coefficient matrix following UPGMA. A dendrogram constructed using the information of the average dissimilarity coefficient matrix of all the ten varieties based on the data of nine RAPD primers placed the varieties in two categories. Cluster analysis grouped six rice varieties i.e. Sarshar, TN-1, Jajai-77, JP-5, Pakhal and Shadab into one group, indicating the similarities between these varieties while the four rice varieties RI-DR-92, Shaheen Basmari, NIR-9 and Sada Hayat were found to be different from the six varieties grouped together. The cluster analysis placed RI-DR-92 and Sarshar in the different groups confirms the maximum genetic distance between the two varieties as shown by the genetic distance (64%) estimated in percent. The dendrogram indicated that the RI-DR-92 and Sarshar and JP-5 and Sada Hayat are distantly apart from one another and can be crossed to broaden the genetic base of Oryza sativa. In addition, more informative primers can be converted to sequence tagged sites (STS) and sequence characterized amplified regions (SCAR) for the amplification of specific alleles which can aid further in rice genome analysis.

Keywords: RAPD marker, Genetic diversity, Polymorphism, Rice, Cultivars

Corresponding author: Faiza Tawab, Department of Botany, Shaheed Benazir Bhutto Women University, Larama Peshawar, Pakistan, Phone: +923336544951, E-mail: drfaizatawab@sbbwu.edu.pk

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Published in „GENETIKA“ Vol. 57, No.1 (2025), pp. 63-75

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2501063K
Original scientific article

MOLECULAR PROFILING AND GENETIC DIVERSITY ASSESSMENT OF RICE (Oryza sativa L.) USING SSR MARKERS

Shruti KUMARI¹, S. K. CHAKRABARTY¹, Praloy KUMAR BHOWMICK², Paul DEBASHIS³

¹Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute,

New Delhi, India

²Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India

³ICAR-Central Institute for Cotton Research, Regional Station, Sirsa, Haryana, India

Abstract

Kumari S., S. K. Chakrabarty, P. Kumar Bhowmick, P. Debashis (2025). Molecular profiling and genetic diversity assessment of rice (Oryza sativa L.) using SSR markers- Genetika, Vol 57, No.1, 63-75.

Evaluating genetic resources for seed vigour traits is crucial for identifying genotypes and traits suitable for enhanced variety development. Simple sequence repeat (SSR) markers have been undertaken for the evaluation of genetic diversity among 44 Indian rice varieties. Among the 41 SSR markers used, 38 markers were found to be 100% polymorphic and three markers, RM 204, RM 20, and RM 125 were found to be monomorphic for one allele but another allele was polymorphic. Out of a total of 141 alleles detected 138 were polymorphic. The number of alleles per locus varied from 2 to 5 with an average of 3.43 allele per locus. The overall size of amplified products varied from 50 base pair (bp) (RM 240) to 600 bp (RM 13). The Polymorphic Information Content (PIC) value of each marker ranged from 0.013 to 0.967, with an average of 0.430. A UPGMA dendrogram based on SSR polymorphism rice varieties were classified into five major clusters (I to V) based on their genetic similarity values and maturity periods, ranging from early to very late. Cluster I contained three early-maturing varieties, cluster II 21 varieties of early and mid-maturity groups, and cluster III 18 late and very late-maturing group varieties. Clusters IV and V had single variety of mid and very late-maturity group. The results can accelerate the creation of high-performing varieties with enhanced seed vigour, promoting improved crop establishment and overall yield potential while enhancing the adaptability of plant varieties to changing environmental conditions.

Key words: Genetic diversity, SSR markers, seed vigour, rice

Corresponding author: S. K. Chakrabarty, Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi, India (E-mail: shyamal kumar chakrabarty@icar.org.in

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Published in „GENETIKA“ Vol. 57, No. 1(2025), pp. 77-96

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2501077I
Original scientific article

SHADING EFFECT ON PERFORMANCES OF CROP GROWTH AND YIELD PARAMETERS OF VINING PEA AT DIFFERENT DEVELOPMENTAL STAGES

Sibel IPEKESEN*and Behiye Tuba BICER

Dicle University, Faculty of Agriculture, Department of Field Crops, Diyarbakir, Turkey

Abstract

Ipekesen S. and B.T.Bicer (2025). Shading effect on performances of crop growth and yield parameters of vining pea at different developmental stages. - Genetika, Vol 57, No.1, 77-96.

In the paper, the effect of different shading treatments (full sunlight, light shading: 40%, medium shading: 75%, and heavy shading: 90%) on yield and crop growth parameters of vining pea was investigated. The experiment was conducted at Dicle University, Faculty of Agriculture, in Diyarbakir, Türkiye during the 2023 spring season under field conditions. The experiment was arranged Randomized Complete Blocks design with three replications. In study, some observations were investigated such as plant height, number of internodes per plant, leaf area per plant, number of leaves per plant, dry matter, SPAD value, soil moisture, number of pods per plant, fresh pod weight, pod width, pod length, number of seeds per pod and dry pod weight and some growth parameters were calculated. For this purpose, plants were harvested six times at 10-day intervals and measured for growth and yield parameters. Consequently, shading treatments increased fresh pod weight, pod width, and pod length compared to full sunlight, while heavy shading (90%) significantly reduced plant height. The crop growth rate tended downward under the shading conditions at the blooming and podding stages. However, crop growth rate was apparently higher under low light intensity (light shading; 6.46 g m^-2 day^-1 and heavy shading; 5.14 g m^-2 day^-1) than at full sunlight (3.35 g m^-2 day^-1) at the podding stage. Full sunlight was the most sensitive treatment to environmental conditions, therefore, the relative growth rate drastically reduced during the crop growth stages. In conclusion, the relative growth rate of crops grown under full sunlight was significantly lower than that of crops grown under other treatments. In summary, light and temperature accelerated crop development under full sunlight, while shade applications extended crop development duration. As a result, medium and low-level shading is thought to extend crop vegetation duration, especially in the arid and semi-arid climate areas exposed to water scarcity and high heat.

Keywords: pea, shade intensity, crop growth, yield, Pearson’s correlation.

Corresponding author: Sibel Ipekesen, Dicle University, Faculty of Agriculture, Department of Field Crops, Diyarbakir, Turkey, Phone: +905341176694, E-mail: sibelisikten@gmail.com

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Published in „GENETIKA“ Vol. 57, No1(2025), pp 97-112

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2501097G
Original scientific article

PROSPECTS FOR BREEDING AND HETEROSIS STUDIES FOR YIELD AND YIELD COMPONENTS IN WHITE LUPINE HYBRIDS (Lupinus. albus L.)

Natalia Anastasova GEORGIEVA¹, Valentin Ivanov KOSEV¹, Vera Miloševa POPOVIĆ^2*

¹Institute of Forage Crops, Pleven, Agriculture academy, Bulgaria

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Georgieva A.N., V. I. Kosev, V.M. Popović (2025). Prospects for breeding and heterosis studies for yield and yield components in white lupine hybrids (Lupinus. albus L.). - Genetika, Vol 57, No.1, 97-112.

The study was conducted in the period 2019-2023 with white lupine cultivars (NORT486, BGR6305 and WAT) and their hybrids (NORT486 × BGR6305; BGR6305 × NORT486; BGR6305 × WAT; WAT × BGR6305) in F1-F3 generations. The following parameters were defined: heterosis effect in F1, inbred depression, degree of dominance in F1 and F2, transgression, dominance, epistasis, coefficient of inheritance in broad sense and narrow sense, coefficient of effectiveness of the mass of genotypes by phenotypical performance of the trait, cytoplasmic effect were determined. The highest positive hypothetical (17.76%-94.73%) and true heterosis (11.0-81.76%) was found in the crosses BGR6305 × NORT486 and BGR6305 × WAT regarding plant height, seeds number, seed weight per plant, 1000 seeds mass, and in BGR6305× WAT – for pods number (97.09%, 90.61%). In F1 of hybrids BGR6305 × NORT486 and BGR6305 × WAT, the inheritance of plant height, seeds weight, and 1000 seeds mass was positively predominant. Negative dominance and predominance were established in WAT × BGR6305 for plant height (-7.41) and 1000 seed mass (-0.69) and in NORT486 × BGR6305 and BGR6305 × NORT486 for pods number (-1.95, -2.99). In most hybrids, epistatic gene interactions played a greater role in the inheritance of traits. The phenotypic expression of the traits of plant height, pods number and seeds number in BGR6305 × WAT, as well as seed weight per plant in BGR6305 × WAT and WAT × BGR6305 was genotypically determined. To achieve faster results in these accessions, multiple individual selection should be carried out in the early hybrid progenies (F2 - F3). Regarding 1000 seeds mass, breeding success (through mass selection) can be achieved in later hybrid generations.

Keywords: white lupine, parental forms, hybrids, genetic analysis

Corresponding author Vera Popović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia, E-mail: drvvpopovic@gmail.com; vera.popovic@ifvcns.ns.ac.rs,

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Published in „GENETIKA“ Vol. 57, No.1 (2025), pp.113-128

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2501113K
Original scientific article

STABILITY AND GENOTYPE X ENVIRONMENT ANALYSIS FOR SEED AND OIL YIELD PARAMETERS AMONG INBRED LINES OF SUNFLOWER

(Helianthus annuus L.)

Vineeta KAILA, S. S. KANDHOLA, Pankaj SHARMA

Oilseeds Section, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana,Punjab, India

Abstract

Kaila V., S. S. Kandhola, P. Sharma (2025). Stability and genotype x environment analysis for seed and oil yield parameters among inbred lines of sunflower (Helianthus annuus L.) - Genetika, Vol 57, No.1, 113-128.

Stability analysis was carried out for 44 sunflower inbreds evaluated for four seasons 2018-2021 using Additive Main Effects and Multiplicative Interaction (AMMI) model, which reveals significant interactions between inbreds and environment for seed yield per plant. Based on AMMI analysis 14 inbreds were found stable for seed yield while 15 inbreds were identified having stable oil yield across environments. The stable inbreds for both the traits as per AMMI analysis were OPH96, OPH98, SF3R, P147R, P107R, OPH118, OPH73, HRAHA5, P121R, P150R, P140R and SF1R. Similarly genotype x environment (G x E) analysis on the same set of inbreds was carried out based on GGE model which lead to discrimination of environments as well as identification of stable inbreds for both seed and oil yield. The superior inbreds identified using GxE biplot were OPH137, OPH76, OPH102 and OPH122. The superior inbreds based on the factor analysis index were OPH 102, OPH 75, OPH 86, OPH 76, P150R2, OPH 122 and OPH 150.

Keywords: AMMI, BLUPs, GGE biplot, Multi Trait Stability Index, Stability

Corresponding author: Vineeta Kaila, Oilseeds Section, Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India, +91 8427564490, vineetakaila@pau.edu

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Published in „GENETIKA“ Vol. 57, No. 1(2025), pp. 129-144

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2501129U
Original scientific article

Fingerprint and expression profiles of OARE – 1 retrotransposon in morphological parts of Avena sativa varieties

Lucia URBANOVÁ¹, Silvia FARKASOVÁ¹, Lenka KUČEROVÁ², Jana ŽIAROVSKÁ^2*

¹Slovak University of Agriculture in Nitra, Research Centre of AgroBioTech, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic

²Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic

Abstract

Urbanová L., S. Farkasová, L. Kučerová, J. Žiarovská (2025). Fingerprint and expression profiles of OARE – 1 retrotransposon in morphological parts of Avena sativa varieties - Genetika, Vol 57, No.1, 129-144.

Avena sativa L. is the most typical cultivated oat species, highly valued as a good source of essential nutrients. Its genome stores a great mass of repetitive elements (86,95%) including Ty1-copia LTR-retrotransposons. Their activation in a stress environment was characterized in tobacco as a defence response. The genomic activity of OARE-1 was studied by PBS, IRAP DNA-based marker techniques and transcriptomic activity of OARE-1 was measured by qPCR in three varieties of oat including analysis of five morphological parts. Both DNA marker techniques were able to describe various profiles, PBS with 49 loci and PIC value of 0,303, IRAP with 218 loci and average PIC value of 0,291. A very variable fingerprint profiles were obtained, and no variety specificity was confirmed. The highest expression level of OARE-1 showed samples of root and the lowest levels of OARE-1 were expressed by chaff samples.

Keywords: common oat; DNA marker technique, PBS, IRAP, qPCR

Corresponding author: Jana Žiarovská, Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences; Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic, tel: +421 37 641 4444, e-mail: jana.ziarovska@uniag.sk

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Published in „GENETIKA“ Vol. 57, No.1 (2025), pp. 145-157

Serbia

UDC 575.630

https://doi.org/10.2298/GENSR2501145B

Original scientific article

GENETIC VARIABILITY AND PCA OF GROWTH AND PHYSIOLOGICAL TRAITS IN Ulmus villosa SEED SOURCES

Gurveer Kaur BRAR, Harmeet Singh SARALCH*, Sapna THAKUR

Department of Forestry and Natural Resources, Punjab Agricultural University, Ludhiana-141004, Punjab, India

Abstract

Brar K. G., H. S. Saralch, S. Thakur (2025). Genetic variability and PCA of growth and physiological traits in Ulmus villosa seed sources- Genetika, Vol 57, No.1, 145-157.

The study investigated growth performance and physiological variability among different seed sources of Ulmus villosa, with the aim of identifying the most productive and site-adapted seed source under field conditions in Punjab. Seeds collected from five sources in Himachal Pradesh—Suket (Mandi), Jhidi (Mandi), Kumi (Mandi), Kullu, and Nauni (Solan) were initially evaluated under nursery conditions and the best performing progenies were transplanted (August 2019) for field evaluation. In terms of growth and physiological traits, significant variations were recorded among the seed sources where progenies of S1 (Suket) and S3 (Kumi) outperformed rest of the sources in all traits and can be exploited for superior progeny selection. Moderate heritability was obtained for majority of the growth and physiological traits and was found maximum for volume index and Chlorophyll a. Similarly, genetic gain was recorded maximum for chlorophyll a followed by volume index. Highly significant positive genotypic and phenotypic correlations were also observed for volume index with DBH and plant height; plant height with DBH; number of branches with number of leaves per plant; and total chlorophyll with chlorophyll b. Principal Component Analysis identified DBH and volume index as the major contributors to diversity among the seed sources, suggesting these traits as priorities for future genetic improvement of the species.

Keywords: Ulmus villosa, seed sources, variability, correlation studies, principal component analysis

Corresponding author: Harmeet Singh Saralch, Department of Forestry and Natural Resources, Punjab Agricultural University, Ludhiana-141004, Punjab, e-mail harmeet@pau.edu

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Published in „GENETIKA“ Vol. 57, No. 1(2025), pp. 159-176

Serbia

UDC 575.

https://doi.org/10.2298/GENSR2501159S

Original scientific article

RISK FACTORS, CLINICAL ASPECTS, AND FOKI POLYMORPHISM OF VITAMIN D RECEPTOR GENE IN PREECLAMPSIA CASES ATTENDING COMBINED MILITARY HOSPITAL, MUZAFFARABAD, AZAD KASHMIR

Iram SHEHZADI¹, Sabika FIRASAT^1*, Kiran AFSHAN¹, Maryam ZUBAIR², Haiba KAUL³

¹Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

²Department of Obstetrics and Gynaecology, Azad Jammu and Kashmir (AJK) Medical College Muzaffarabad, AJK, Pakistan

³Genetics Division, Department of Livestock Production, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki, Pakistan

Abstract

Shehzadi I., S. Firasat, K. Afshan, M.Zubair, H. Kaul (2025). Risk factors, clinical aspects, and FOKI polymorphism of vitamin D receptor gene in preeclampsia cases attending combined military hospital, Muzaffarabad, Azad Kashmir - Genetika, Vol 57, No.1, 159-176.

Preeclampsia (PE) is a pregnancy-related disorder. The current research was performed to study the epidemiology, clinical characteristics and predictive role of reproductive and demographic risk factors in the development of preeclampsia (PE). Furthermore, a FOKI sequence variant of VDR gene (rs2228570) was analyzed to see its association with pre-eclampsia phenotype.Current case-control study comprising of PE patients and age-matched controls recruited from the Gynecology department of Combined Military Hospital (CMH), Muzaffarabad, Azad Kashmir was conducted from August 2021 to January 2022 at Quaid-i-Azam University Islamabad, Pakistan. A predesigned questionnaire was used to collect socio-demographic information, clinical parameters, reproductive health, family history of hypertension, and various lifestyle factors from PE patients and controls. SPSS 20 was used to analyze the data. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP-PCR) method was executed on extracted DNA samples of PE patients to examine the genotypes for VDR FOKI polymorphism. Out of 1933 pregnant women who delivered at CMH, Muzaffarabad during April to July, 2021, 3.10% were diagnosed with PE. A total of 300 pregnant females were recruited for this case control study out of which 60 were PE patients and 240 were age matched controls. SPSS analysis of data showed that family history of hypertension and previous miscarriage history were strongly associated with high risk of PE (p<0.05), whereas multiparity and proper supplements intake during pregnancy were strongly associated with decreased risk of PE in AJK women (p<0.05). Clinical characteristics i.e., Systolic blood pressure (SBP), Diastolic blood pressure (DBP), Alkaline Phosphatase (ALP), Alanine Transaminase (ALT) and urinary proteins were found significantly elevated while blood haemoglobin was significantly lower in PE patients (p<0.0001). Frequency of minor allele of FOKI polymorphism was higher in PE patients but no significant association was detected. PE had a frequency of 3.10% among pregnant females presented at CMH, Muzaffarabad. Identification of positive association of family history of hypertension, previous miscarriage history with PE onset and high frequency of FOKI minor allele in PE patients reaffirms their involvement in PE etiology.

Key words: Pre-eclampsia, risk factor analysis, miscarriage, hypertension, FOKI polymorphism, VDR gene

Corresponding author: Sabika Firasat, Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, University Road, 45320, Islamabad, Pakistan. Phone:0925190644410, email:sabika.firasat@qau.edu.pk

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