GENETIKA, Vol

GENETIKA, Vol. 57, No3 (2025)

Zahra GHARAGHEIZI, Masoud SHEIDAI, Zahra NOORMOHAMMADI, Naser KALHOR

A PRELIMINARY REPORT ON POPULATION GENETICS ANALYSIS OF TWO CRISP2 GENE’ SNPS IN RELATION TO ASTHENOSPERMIA INFERTILITY: COMPUTATIONAL AND PHYLOGENETIC APPROACHES [Abstract] [Full text]

Gaurav KHOSLA, B S GILL and Pankaj SHARMA

GENETIC USEFULNESS OF MULTI-PARENTAL CROSSES VIS-À-VIS BI-PARENTAL CROSSES IN SOYBEAN [Abstract] [Full text]

Mirjana JOVOVIĆ, Verica MILOVIĆ, Zoranka MALEŠEVIĆ, Marko PETKOVIĆ

THE INFLUENCE OF HUMIC ACID APPLICATION ON TOMATO CULTIVARS UNDER GREENHOUSE CONDITIONS [Abstract] [Full text]

Abul Awlad KHAN

DROUGHT TOLERANCE INDICES: GENETIC PARAMETER ESTIMATION AND APPLICATION IN DISCRIMINATING TOLERANT WHEAT GENOTYPES [Abstract] [Full text]

SHWETA and Mohit KUNDAL

GENETIC VARIABILITY IN SEEDLING CHARACTERISTICS OF Bauhinia vahlii Wight & Arn. [Abstract] [Full text]

Natalia ANASTASOVA GEORGIEVA, Valentin IVANOV KOSEV, Vera M. POPOVIĆ, Jelena GOLIJAN PANTOVIĆ, Jela IKANOVIĆ
RESPONSES OF WHITE LUPINE ACCESSIONS TO ENVIRONMENTAL STRESSES UNDER ORGANIC CULTIVATION [Abstract] [Full text]

Hameed Akande BASHIRU and Saidu Oyarekhua OSENI

GENETIC INFLUENCE OF GROWTH HORMONE RECEPTOR (GHR) POLYMORPHISMS ON RABBIT BODY WEIGHT: A META-ANALYSIS [Abstract] [Full text]

Manisha THAKURand Hament THAKUR

VEGETABLE BREEDING FOR ORGANIC FARMING: OPPORTUNITIES AND PERSPECTIVES [Abstract] [Full text]

Published in „GENETIKA“ Vol. 57, No3(2025), pp. 333-350

UDC 575.
https://doi.org/10.2298/GENSR240903002G

Original scientific article

A PRELIMINARY REPORT ON POPULATION GENETICS ANALYSIS OF TWO CRISP2 GENE’ SNPS IN RELATION TO ASTHENOSPERMIA INFERTILITY: COMPUTATIONAL AND PHYLOGENETIC APPROACHES

Zahra GHARAGHEIZI¹, Masoud SHEIDAI^2*, Zahra NOORMOHAMMADI¹, Naser KALHOR³

¹Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

³Department of mesanchymal stem cell, Academic Center for Education, Culture and Research, Qom branch, Iran

Abstract

Gharagheizi Z., M. Sheidai, Z. Noormohammadi, N. Kalhor (2025): A preliminary report on population genetics analysis of two CRISP2 gene’ SNPS in relation to asthenospermia infertility: Computational and phylogenetic approaches.- Genetika, Vol 57, No. 3, 333-350.

Asthenospermia (AZS) is a cause of infertility in men in which the motility of the sperm is reduced. CRISP2 genes’ SNPs may be involved in this type of infertility and reach on association studies for these SNPs are going on in different parts of the world. The present study was conducted to investigate association between two SNPs of gene CRISP2 namely, rs1301440109, and rs-760204944, with asthenospermia in Iranian samples. For this study, 83 infertile men suffered from asthenospermia and 40 fertile males with normal karyotype and semen analysis were studied, by using tera-arms method. We performed different computation methods on both genetic data, sequences, as well as clinical features related to sperm characteristics. Genotypig of the studied samples showed the presence of two different alleles in both SNPs studio. The frequency of the genotypes and the alleles obtained are presented, with T alleles as the most frequent allele in both SNPs studied. Logistic regression showed only a significant association between rs1301440109 and infertility. Sequencing of the target SNPs along with their neighboring sequences, compared to the ancestral sequences, showed a significant sequence divergence for the indigenous individuals with positive significant Tajimas’ D statistic showing adaptive change in these sequences for the rs 1301440109. Linear discriminant analysis (LDA), differentiated the case and control individuals in the rs1301440109, based on clinical features studied. Redundancy analysis (RDA), did not produce a significant association between the genotypes obtained and geographical variables.

Key words: Asthenospermia, Iran, Logistic regression, RDA, sequence divergence, SNPs

Corresponding author: Masoud Sheidai, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran, email: msheidai@yahoo.com,

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Published in „GENETIKA“ Vol. 57, No. 3 (2025), pp.351-361

Serbia

UDC 575.633
https://doi.org/10.2298/GENSR240817004K

Original scientific article

GENETIC USEFULNESS OF MULTI-PARENTAL CROSSES VIS-À-VIS BI-PARENTAL CROSSES IN SOYBEAN

Gaurav KHOSLA, B S GILL and Pankaj SHARMA

Department of Plant Breeding & Genetics

Punjab Agricultural University, Ludhiana, India

Abstract

Khosla G., B S Gill and P. Sharma (2025): Genetic usefulness of multi-parental crosses vis-à-vis bi-parental crosses in soybean.- Genetika, Vol 57, No. 3, 351-361.

Advanced breeding lines from seven different crosses were evaluated in randomized Block Design during Kharif 2016 and 2017 for the study of genetic parameters. High broad sense heritability (H_bs) and genetic advance as per cent of mean (GA_PM) were observed for seed yield per plant, biomass per plant and number of pods per plant that depicts additive gene action and hence selection based on these traits would be more reliable. Multi-parental crosses had in general higher estimates of heritability and genetic advance as compared to bi-parental crosses. Genetic usefulness (U) for yield contributing traits like pods per plant, 100 seed weight was higher in cross 2 whereas bi-parental crosses had high genetic usefulness for seed yield and biological yield per plant. Susceptibility of parental lines might have resulted in lower genetic usefulness in multi-parental crosses. Selection of diverse parents to be used in breeding programme should be undertaken while considering the susceptibility of parental lines to various biotic and abiotic factors responsible for yield losses.

Key words: genetic advance, genetic usefulness, heritability, multi-parental, soybean

Corresponding author: Gaurav Khosla, Department of Plant Breeding & Genetics Punjab Agricultural University, Ludhiana, India, email: goruvkhosla@gmail.com, (+9198159-65404),

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Published in „GENETIKA“ Vol. 57, No.3 (2025), pp.363-375

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR250411007J

Original scientific article

THE INFLUENCE OF HUMIC ACID APPLICATION ON TOMATO CULTIVARS UNDER GREENHOUSE CONDITIONS

¹Mirjana JOVOVIĆ, ¹Verica MILOVIĆ, ^{2 1}Zoranka MALEŠEVIĆ, ³Marko PETKOVIĆ

¹Faculty of Agriculture, University of East Sarajevo, Bosnia and Herzegovina

²Academy of Vocational Studies Sumadija, Arandjelovac, Serbia,

³Faculty of Agronomy, University of Kragujevac, Serbia

Abstract

Jovović M., V. Milović, Z. Malešević, M.Petković (2025): The influence of humic acid application on tomato cultivars under greenhouse condition.- Genetika, Vol 57, No. 3, 363-375.

Responses of two tomato (Lycopersicon esculentum L) hybrid genotypes Rally F1 (a1) and Pink Rock F1 (a2) to various doses (0 (b1), 0.5 (b2), 1.0 (b3), and 1.5 g L^-1(b4)) of soil application of humic acid (HA) were evaluated in terms of yield traits and quality of fruits, under greenhouse conditions in 2022. The results indicated that soil drenching with different concentrations of humic acid improved fruit productivity and quality in both tomato hybrids. The application of various biostimulant concentrations did not have a statistically significant effect on fruit weight, length, diameter, and yield, nor on the content of citric and malic acids, regardless of the tomato genotype. In contrast, the application of different biostimulant concentrations influenced the content of total acids, total sugars, dry matter, and vitamin C. The a1b3 product was the highest quality product according to the analyzed parameters. The a2 genotype produced a higher-quality product compared to the a1 genotype, without the use of biostimulants (b1). A high negative correlation coefficient was observed between vitamin C and total acids (r = -0.82), vitamin C and malic acids (r = -0.88), total sugars and total acids (r = -0.69), and total sugars and oxalic acid (r = -0.82). PCA analysis showed that the statistically significant responses of the analyzed parameters depend more on changes in the concentration of biostimulants than on the genotype.

Key words: genotype, humic acid, fruit quality, tomato, yield

Corresponding author: Mirjana Jovović, University in East Sarajevo, Faculty of Agriculture, e-mail:mirjanamojevic@gmail.com, +38765094774

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Published in „GENETIKA“ Vol. 57, No3(2025), pp.377-397

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR2503377K

Original scientific article

DROUGHT TOLERANCE INDICES: GENETIC PARAMETER ESTIMATION AND APPLICATION IN DISCRIMINATING TOLERANT WHEAT GENOTYPES

Abul Awlad KHAN*

Abstract

Khan A. A. (2025): Drought tolerance indices: genetic parameter estimation and application in discriminating tolerant wheat genotypes.- Genetika, Vol 57, No. 3, 377-397.

The drought incidence and severity are one of the majors constrain in wheat production and grain yield in Bangladesh. Therefore, this work aimed to evaluate the use of drought tolerance indices for screening spring wheat genotypes with stability for a specific environment. Accordingly, field experiments were conducted with 35 advance wheat genotypes during 2020-2021 at the Bangladesh Wheat and Maize Research Institute, Rajshahi under two field environments: rain-fed and irrigated conditions. The indices Tol, SSI, SSPI, SDI and STI showed moderate to high heritability with genetic advances indicating possible improvement through selection. The genotypic (r_g) and phenotypic (r_p) correlations coupled with principal component analysis (PCA) depicted that the indices MP, HM, GMP, STI, YI and DI could be considered as the better predictor of potential yield under both non stress and drought stress environments. Besides, the indices Tol, SSI, SSPI, SDI, YSI and RDI could screen highly susceptible and low susceptible genotypes that can be recommended for optimum environment and stress environment, respectively. Finally, the genotypes G-10, G-16, G-20 and G-33 were selected as a source of drought tolerance and could be used either in the breeding program for varietal improvement or forwarded to advanced multi-location trial in drought prone areas.

Key words: correlations, drought tolerance, genetic variability, principal component analysis, spring wheat

Corresponding author: Abul Awlad Khan, Bangladesh Wheat and Maize Research Institute, Wheat Breeding Division Nashipur, Dinajpur-5200, Bangladesh, email: aakhanwrc@gmail.com, phone: 02588817745

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Published in „GENETIKA“ Vol. 57, No.3 (2025), pp. 399-406

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR240826005S

Original scientific article

GENETIC VARIABILITY IN SEEDLING CHARACTERISTICS OF Bauhinia vahlii Wight & Arn.

SHWETA* and Mohit KUNDAL

Department of Tree Improvement and Genetic Resources, College of Forestry,

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India

Abstract

Shweta and M. Kundal (2025): Genetic variability in seedling characteristics of Bauhinia vahlii wight & arn.- Genetika, Vol 57, No. 3, 399-406.

Bauhinia vahlii Wight and Arn. belongs to the family Caesalpiniaceae and is commonly known as Maloo creeper or Toor. It is one of the important species whose leaves are even used as fodder and are commercially used as donas and pattals. It thrives well in the sub-Himalayan region ranging upto 1500 meters above mean sea level. The fruits (pods) were collected from different seed sources of Himachal Pradesh including four districts viz. Solan, Sirmour, Hamirpur and Kangra. Analysis of variance for different seedling characters revealed the significant variations. Highly significant correlation was found between collar diameter and other growth characters namely seedling height, internodal length and number of leaves in half-sib progenies. The genetic estimates worked out for mother tree leaf area and leaf length has shown promising possibilities for heritability (0.35) and genetic gain (10.15) respectively. The nursery growth performance of seedling height was estimated to show accountable genetic gain (16.17 %) and high heritability (0.68) for leaf length. The present study concluded that four seed source viz., Bhojnagar, Dharbanar, Kathar and Tauni Devi have most of the scalable performances on key characters like leaf foliage of half sibs as well as seedling growth potentials. These seed sources could further be evaluated at field conditions for effective selection of superior mother trees for further breeding programmes.

Key words: Bauhinia vahlii, correlation, genetic gain, heritability, pod

Corresponding author: Shweta, Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India, email: shwetaramgarhia95@gmail.com

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Published in „GENETIKA“ Vol. 57, No. 3(2025), pp. 407-424

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR250328003A

Original scientific article

RESPONSES OF WHITE LUPINE ACCESSIONS TO ENVIRONMENTAL STRESSES UNDER ORGANIC CULTIVATION

Natalia ANASTASOVA GEORGIEVA¹, Valentin IVANOV KOSEV¹, Vera M. POPOVIĆ²^*, Jelena GOLIJAN PANTOVIĆ³, Jela IKANOVIĆ⁴

¹Institute of Forage Crops, Pleven, Agriculture academy, Pleven, Bulgaria

² Institute of Field and Vegetable Crops, Novi Sad, Serbia

³University of Belgrade; Faculty of Agriculture, Zemun-Belgrade, Serbia

⁴IPN- Institute for Science Application in Agriculture, Belgrade, Serbia

Abstract

Georgieva Anastasova N., V. Ivanov Kosev, V. M. Popović, Jelena Golijan Pantović, Jela Ikanović (2025): Responses of white lupine accessions to environmental stresses under organic cultivation.- Genetika, Vol 57, No. 3, 407-424.

In the present study, a comparative assessment of 15 white lupine accessions was made regarding productivity, stability, biochemical composition, and tolerance to Fusarium wilt under organic cultivation conditions. The results showed that accession Lp 425 was distinguished by the highest values in terms of plant height (90.3 cm), average daily growth rate (0.84 cm/day), aboveground biomass (48.23 g/plant) and seed productivity (20.93 g/plant). According to the values of the stability parameter YSi, Lp 01 (15.00+) and Lp 425 (14.00+) exhibited ecological stability. The same accessions were also characterized by a short vegetation period (109 days). Regarding seed quality, increased protein content was demonstrated by Lp 27/10, Lp 21, and Lp 125 (367.15-371.95 g/kg DM), and tolerance to F. oxysporum – by Lp 251, Lp 27/7, and Lp 125.The complex assessment regarding main quantitative characteristics, stability and vegetation period determined Lp 425 (rank 1) as the most suitable for organic growing. Despite the relatively high percentage of infected plants and disease severity score (compared to the others), Lp 425 realized the highest productivity. A good evaluation, although with a lower rank (2), was received by Lp 01.

Key words: accessions, Fusarium wilt, productivity, stability, white lupine

Corresponding author: Valentin Kosev, Institute of Forage Crops, Pleven, Agriculture academy, Pleven, Bulgaria, valkosev@abv.bg, Vera Popovic, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia

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Published in „GENETIKA“ Vol. 57, No3(2025), pp 425-442

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575.
https://doi.org/10.2298/GENSR2503425B

Original scientific article

GENETIC INFLUENCE OF GROWTH HORMONE RECEPTOR (GHR) POLYMORPHISMS ON RABBIT BODY WEIGHT: A META-ANALYSIS

Hameed Akande BASHIRU* and Saidu Oyarekhua OSENI

Department of Animal Sciences, Faculty of Agriculture, Obafemi Awolowo University,

Ile-Ife, 220282, Nigeria

Abstract

Bashiru H. A. and S. O. Oseni (2025): Genetic influence of growth hormone receptor (ghr) polymorphisms on rabbit body weight: A meta-analysis- Genetika, Vol 57, No. 3, 425-442.

Growth and metabolism regulation in rabbits are influenced by the growth hormone receptor (GHR). However, despite its promising potential as candidate for marker assisted section, the association between GHR gene polymorphisms and body weight under different genetic models remains inconclusive. This study deployed systematic review and meta-analysis to assess this relationship. The protocol was retrospectively registered on the Open Science Framework. Following the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) guidelines, 17 data points from six studies were analyzed. Hardy-Weinberg Equilibrium (HWE) was tested, and four genetic models (co-dominant, dominant, recessive, and over-dominant) were fitted using OpenMeta® Analyst Software. Standardized mean differences (SMDs) were estimated using Cohen’s d method, with heterogeneity assessed via Q statistic, Tau-squared (τ²), H-squared (H²), and I-squared (I²). Egger’s regression test was used to evaluate publication bias and sensitivity analysis was conducted to determine single-study effects. There was significant association (p<0.05) of GHR gene with body weight of rabbits under one of the co-dominant models (CG vs GG). However, this association was not significant (p>0.05) under all other genetic models. Further, the results indicated that GG genotype had higher body weight than CG genotype. However, there was no difference (p>0.05) between CC and CG and CC and GG genotypes. This suggests that GHR gene polymorphisms have a limited influence on body weight and their impact may depend on specific genetic models.

Key words: candidate genes, genetic markers, genetic models, meta-analysis, SNP analysis

Corresponding author Hameed Akande Bashiru, Department of Animal Sciences, Faculty of Agriculture, Obafemi Awolowo University, Ile-Ife, 220282, Nigeria, Email: hbashiru@oauife.edu.ng, Phone: +2347037292065

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Published in „GENETIKA“ Vol. 57, No3 (2025), pp.443-456

Serbia

UDC 575.630
https://doi.org/10.2298/GENSR240909006T

Review article

VEGETABLE BREEDING FOR ORGANIC FARMING: OPPORTUNITIES

AND PERSPECTIVES

Manisha THAKUR¹and Hament THAKUR²

¹School of Organic Farming and ²Regional Research Station Ballowal Saunkhari Punjab Agricultural University Ludhiana, Punjab, India

Abstract

Thakur M.and H. Thakur (2025): Vegetable breeding for organic farming: opportunities and perspectives.- Genetika, Vol 57, No. 3, 443-456.

Vegetable breeding for organic farming is the science and practice of developing new varieties suitable for organic production systems. The main aim is to enhance the potential of organic farming through development of new varieties. It is a holistic approach that respects natural crossing barriers and based on fertile plants that can establish a viable relationship with the living soil. A variety suitable for organic farming is obtained by breeding methods that are in compliance with the organic farming concept and is the result of a certified organic plant breeding programme. Breeding organic vegetable varieties is essential for the development of the organic vegetable sector and for the quality of organic vegetables. But at the same time it is an ambitious striving because it is not yet realised on a large scale, and still very much in development. Breeding vegetables for organic farming requires a systematic approach that considers the complexity of natural ecosystems and operates across multiple levels of plant biology.

Key words: biodiversity, local genotypes, methods and strategies, specific traits, sustainability, vegetable breeding

Corresponding author: Manisha Thakur, School of Organic Farming, Punjab Agricultural University Ludhiana, Punjab, India, email: mnshthakur4@pau.edu

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