L. EHRENBERG, Dobrila ĐORĐEVIĆ, Katarina LINDGREN and A.T. NATARAJAN
TIME OF DNA SYNTHESIS DURING GERMINATION IN DIFFERENT VARIETIES OF TWO-ROWED BARLEY [Abstract]
Vesna POPOVIĆ, M. DENIĆ and J. DUMANOVIĆ
INFLUENCE OF THE OPAQUE-2 GENE ON PROTEINS IN MAIZE OF DIFFERENT GENETIC BACKGROUNDS [Abstract]
N. PEŠEV
SOME PRELIMINARY OBSERVATIONS ON A NEW SOURCE OF THE OPAQUE-2 MUTANT IN MAIZE [Abstract]
A.L. HOOKER
SELECTION FOR STALK ROT RESISTANCE IN CORN AND ITS EFFECT ON GRAIN YIELD [Abstract]
B.K. MUKHERJEE, K.N. AGARWAL, S.B. SINGH, N.P. GUPTA and N.N. SINGH
STUDIES ON DIVERSE GERM PLASM COMPLEXES OF MAIZE. I. GENE EFFECTS AND NATURE OF HETEROSIS IN GERMPLASM COMPLEXES AND THEIR CROSSES [Abstract]
B.K. MUKHERJEE, K.N. AGARWAL, S.B. SINGH, N. P. GUPTA and N.N. SINGH
STUDIES ON DIVERSE GERM PLASM COMPLEXES OF MAIZE II. PREDICTED AND ACTUAL PERFORMANCES OF ADVANCED GENERATIONS OF TWO-, THREE-, AND FOUR-PARENT MULTIPLE CROSSES [Abstract]
N.N. SINGH, N.L. DHAWAN and B.K. MUKHERJEE
GENETIC STUDIES IN GERM PLASM COMPLEXES IN MAIZE [Abstract]
V. GUZINA
ISOENZYMES OF PEROX1DASES IN GENETIC STUDIES OF POPLARS [Abstract]
Radmila METLAŠ, Sabaha POPIĆ, A. BEĆAREVIĆ and D. KANAZIR
EFFECTS OF SODIUM FLUORIDE ON IN VITRO PROTEIN SYNTHESIS BY RAT LIVER POLYSOMES [Abstract]
B. GEORGI, A.K. KAUL and D. CHRISTOFFERS
USE OF (11, 2n) ACTIVATION - ANALYSIS FOR THE DETERMINATION OF THE NITROGEN CONTENT IN SEED GRAINS [Abstract]
D. CHRISTOFFERS, A.K. KAUL and B. GEORGI
DETERMINATION OF LYSINE IN GRAIN-MEAL SAMPLES, BY DANSYLATION [Abstract]
A.K. KAUL, H.W. KASTNER, B. GEORGI and D. CHRISTOFFERS
DETERMINATION OF RELATIVE NUTRITIVE VALUE (RNV) OF GRAIN SAMPLES WITH TETRAHYMENA PYRIFORMIS (W) [Abstract]
Vlasta HITREC i Ljiljana ZERGOLLERN
A CYTOGENETICAL STUDY OF G-LIKE CHROMOSOMES [Abstract]
Dubravka MUŽINIĆ and Ljiljana ZERGOLLERN
DENATURATION AS THE METHOD OF IDENTIFICATION OF CHROMOSOMES IN TRANSLOCATION [Abstract]
Milena ĆIRIĆ
IDENTIFICATION OF THE PHILADELPHIA (Ph') CHROMOSOME BY A G - BAND METHOD [Abstract]
Mira TUNKL and Ljiljana ZERGOLLERN
THYROID AUTOANTIBODIES IN CHILDREN WITH DOWN'S SYNDROME AND IN THEIR PARENTS [Abstract]
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 1-6
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
TIME OF DNA SYNTHESIS DURING GERMINATION IN DIFFERENT VARIETIES OF TWO-ROWED BARLEY
L. EHRENBERG, Dobrila ĐORĐEVIĆ, Katarina LINDGREN and A.T. NATARAJAN
Wallenberg Laboratory, University of Stockholm, Stockholm, Sweden
Abstract
Erhenberg, L., Đorđevic, D., Lindgren, K. and Natarajan, A. T. (1974): Time of DNA synthesis during germination in different varieties of two-rowed barley. - Genetika, Vol. 6, JY° 1, 1-6.
The time of the first DNA synthesis during germination of barley kernels exhibits a strong variation between varieties and between cultivation years. In cv. Kristina and its parents (Domen and Mari) as well as some other varieties, DNA synthesis starts early, partly already in spike, before harvest and occurs in different cells during a long time, whereas in Bonus it occurs nearly synchronously 20 - 24 h after onset of germination.
These variations explain the
variable success of efforts to induce mutation treatment of germinating kernels
with base analogues and radioactive precursors. Such experiments, as well as
studies of variations in sensitivity to mutagenic treatment, requires, for reproducibility,
phytotron cultivated material, and a characterization of the variety under
study with respect to the time of
DNA synthesis.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 7-17
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
INFLUENCE OF THE OPAQUE-2 GENE ON PROTEINS IN MAIZE OF DIFFERENT GENETIC BACKGROUNDS
Vesna POPOVIĆ, M. DENIĆ and J. DUMANOVIĆ
Department of Genetics and Plant Breeding, INEP, 11080 Zemun, Yugoslavia
Abstract
Popović, V., Denić, M. and Dumanović, J. (1974): Influence of the opaque-2 gene on proteins in maize of different genetic backgrounds. - Genetika, Vol. 6, No. 1, 7-17.
The influence of the opaque-2 gene on protein content and amino acid composition in the endosperm of different inbred lines was studied. It was found that opaque-2 gene reduces protein content and 1000-grain weight. The extent of gene action in reducing these characters was different in different inbred lines. Concerning the fractions separated by electrophoresis on polyacrylamide gel there was an additional poly-peptide around 20,000 MW in the normal genotype, and small one around 40,000 MW in the opaque-2 mutant. Amino acid analysis showed that polypeptides of higher MW contribute to the increased lysine content in the mutant. The same analysis of the overall endosperm showed that in all inbred lines with incorporated opaque-2 gene the amounts of lysine, arginine, aspartate, glycine, cystine, histidine, threonine and valine were remarkably increased, whereas the amounts of leucine, glutamate, alanine, phenylalanine, proline, methionine and tyrosine were decreased.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 19-26
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
SOME PRELIMINARY OBSERVATIONS ON A NEW SOURCE OF THE OPAQUE-2 MUTANT IN MAIZE
N. PEŠEV
Maize Research Institute, 11080 - Zemun, Yugoslavia
Abstract
Pešev, N. (1974): Some preliminary observations of a new source of the opaque-2 mutant in maize. Genetika, Vol. 6. No. 1, 19-26.
Preliminary tests have shown that homozygous seeds of a new source of opaque-2 mutant, derived from two ears of a synthetic of 10 divergent populations, have considerably higher lysine content than seeds with normal endosperm. These opaque-2 seeds had 68 and 85% more lysine than normal seeds, and 17-25% more lysine and 1.3 - 1.8% more protein than the opaque-2 Purdue mutant. Segregation of normal and opaque phenotypes was regular and similar to that encountered with the Purdue mutant.
The results obtained showed that changes in level and pattern of protein and lysine take place in the endosperm in this new strain of opaque-2 corn, as well. This source of maize o2 gene probably originate from Yugoslavs varieties included in this synthetic. Our o2' strains showed increased ribonuclease activity 3.14-10.24 times more than inbred lines they originated.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 27-32
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
SELECTION FOR STALK ROT RESISTANCE IN CORN AND ITS EFFECT ON GRAIN YIELD
A.L. HOOKER
Illinois Agricultural Experiment Station, Urbana, Illinois 61801, U.S.A.
Abstract
Hooker, A. L. (1974): Selection for stalk rot resistance in corn and its effect on grain yield. - Genetika, Vol. 6, No. 1, 27-32.
Selection for resistant, intermediate, and susceptible reactions to stalk rot caused by Diplodia maydis was effective during five or seven generations of selfing within seven corn populations. Selection was most effective prior to the fourth generation of selfing. Performance trials with the selected lines crossed with unrelated inbred testers revealed that hybrids produced by the resistant or intermediate lines were superior to those produced by the susceptible lines in grain yield and plant standability.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 33-41
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
STUDIES ON DIVERSE GERM PLASM COMPLEXES OF MAIZE. I. GENE EFFECTS AND NATURE OF HETEROSIS IN GERMPLASM COMPLEXES AND THEIR CROSSES
B.K. MUKHERJEE, K.N. AGARWAL, S.B. SINGH, N.P. GUPTA and N.N. SINGH
Division of Genetics, I.A.R.I., New Delhi, India
Abstract
Mukherjee, B. K., Agarwal, K. N., Singh, S. B., Gupta, N. P. and Singh, N. N. (1974): Studies on diverse germ plasm complexes of maize. I. Gene effects and nature of heterosis in germ plasm complexes and their crosses. - Genetika, Vol. 6, No. 1, 33-41.
Gene effects studies were carried out in five diverse germ plasm complexes of maize namely, J236, Antigua Gr. 1, St. Croix 4D, Cuba 19 and Caribbean Flint, presently being used in the maize breeding programme in India. Heterotic effects were also examined in the various combinations. Epistatic effects were of considerable magnitude in most of the characters studied, supporting the earlier findings of Moll et al., (1965) and Sentz et al., (1954). High additive effects were possessed by Caribbean Flint for grain yield, Antigua Gr. 1 for days to flower and number of kernel rows/ear and J236 for ear length and ear diameter. The average heterosis of all the F1 's was poor in the case of ear length. Cuba 19 and Caribbean Flint possessed significant average heterosis for grain yield and number of kernel rows/ear. Antigua Gr. 1 was found to be promising for transmitting earliness to its crosses.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 43-50
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
STUDIES ON DIVERSE GERM PLASM COMPLEXES OF MAIZE II. PREDICTED AND ACTUAL PERFORMANCES OF ADVANCED GENERATIONS OF TWO-, THREE-, AND FOUR-PARENT MULTIPLE CROSSES
B.K. MUKHERJEE, K.N. AGARWAL, S.B. SINGH, N. P. GUPTA and N.N. SINGH
Division of Genetics, I.A.R.I., New Delhi, India
Abstract
Mukherjee, B. K., Agarwal, K. N., Singh, S. B, Gupta, N. P. and Singh, N. N. (1974): Studies on diverse germ plasm complexes of maize. II. Predicted and actual performances of advanced generations of two-, three-, and four-parent multiple crosses. - Genetika, Vol. 6, No. 1, 43-50.
The article deals with an investigation of the prediction of varietal crosses, their random mated and selfed progenies and advanced generations of three-way multiple crosses derived from five highly heterozygous and diverse varieties of maize. Methods available for the prediction of double cross hybrids utilize the means of highly homozygous inbred lines and single crosses thereof. In this study, however, gene effects have been used to predict the performance of the earlier mentioned po-populations. This study also shows the extent of heterosis obtained and the relative depression of random mated and selfed progenies of the varieties and varietal crosses. In general, there was fairly good agreement between the predicted and actual performance with regard to all the characters studied. A number of varietal crosses were found to be promising in comparison to the best released hybrid. Similarly, a number of three-parent and four-parent multiple crosses gave predicted performances significantly better than the best released hybrid.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 51-62
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
GENETIC STUDIES IN GERM PLASM COMPLEXES IN MAIZE
N.N. SINGH, N.L. DHAWAN and B.K. MUKHERJEE
Division of Genetics I.A.R.I., New Delhi, India and
Ford Foundation, Sharia Osiris, Garden City, Cairo, U.A.R.
Abstract
Singh, N. N., Dhawan, N. L. and Mukherjee, B. K. (1974): Genetic studies in germ plasm complexes in maize. - Genetika, Vol. 6, No. 1, 51-62.
Nineteen germ plasm complexes were top crossed with each of the three testers and line x tester analysis according to Kempthorne (1957), was carried out. Combining ability analysis revealed that both additive and non-additive components of genetic variance were important in the expression of grain yield. For moisture percentage in grains the specific combining ability variance was considerably larger than the general combining ability variance and thus non-additive variance played an important role in the expression of this character, while the reverse was the case for days to silk. The study of interaction of combining ability variance with years indicated that general combining ability x year variance was larger than specific combining ability x year variance for grain yield per plant and moisture percentage in grains. For days to silk, the opposite was found to hold good. Complexes, namely, Antigua 3D, Cuba 19, St. Croix 4D and Caribbean Dent gave high values for general combining ability effects. Antigua 3D x Composite A6 gave the highest value for specific combining ability effect. These complexes can be used for the development of better composite populations as well as for the development of Fj varietal hybrids for direct commercial use. Developed composites can also be used as base populations for generating elite inbreds for the hybrid programme.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 63-68
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
ISOENZYMES OF PEROX1DASES IN GENETIC STUDIES OF POPLARS
V. GUZINA
Institut za topolarstvo, 21000 Novi Sad, Jugoslavija
Abstract
Guzina, V. (1974): Isoenzymes of peroxidases in genetic studies of poplars. - Genetika, Vol. 6, No. 1, 63-68.
Using the method of zonal electrophoresis on starch gel and the buffer system of Poulik (1957), a study was made of the individual variability of young plants of a half-sib family of Populus tremula L. in relation to the zymograms (isoenzyme patterns) of their isoperoxidases.
According to the presence or absence of individual bands in the zymograms of individual plants, 14 different zymograms were found, indicating the presence of 14 different genetic groups of plants with respect to the character of their peroxidases.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 69-76
© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
EFFECTS OF SODIUM FLUORIDE ON IN VITRO PROTEIN SYNTHESIS BY RAT LIVER POLYSOMES
Radmila METLAŠ, Sabaha POPIĆ, A. BEĆAREVIĆ and D. KANAZIR
Laboratory of Biochemistry, »Boris Kidrič« Institute, 11000 Beograd, Yugoslavia
Abstract
Metlas, R., Popic, S., Becarevic, A. and Kanazir, D. (1973): Effects of sodium fluoride on in vitro protein synthesis by rat liver polvsomes. - Genetiika, Vol. 6, No. 1, 69-76.
Polysomes isolated from rat liver when tested for in vitro protein synthesis in the presence of sodium fluoride, show lower amino acid incorporation activity. The same inhibitor effect was observed at NaF concentrations 0.1 and 20 mM. The concentration of 0.01 rnM was not inhibitory.
Protein synthesis with isolated rat liver polysomes inhibited with NaF could be restored by addition of a pH 5 supernatant fraction. Contrary to in vivo findings, sodium fluoride added to incubation mixtures for in vitro protein synthesis did not cause significant disaggregation of polysomes.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 79-87
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
USE OF (11, 2n) ACTIVATION - ANALYSIS FOR THE DETERMINATION OF THE NITROGEN CONTENT IN SEED GRAINS
B. GEORGI, A.K. KAUL and D. CHRISTOFFERS
Institut für Biophysilk, TU Hannover und
Institut für Strahleribotanik der Gesellschaft für Stahlen- und Umweltforschung mbH, Hannover, BRD
Abstract
Georgi, B., Kaul, A. K. and Christoffers, D. (1974): Use of (n, 2n) activation - analysis for the determination of the nitrogen content in seed grains. - Genetika, Vol. 6, No. 1, 79-87.
A BBC neutron generator (600 kV, max. 5 mA) was used to quantitatively measure the nitrogen content in urea (liquid) and assorted grain samples with (n, 2n) reaction. The ideal conditions for sample preparation and measurement are discussed. A correlation coefficient of 0.98** was obtained between the Kjeldahl nitrogen content and (n, 2n) pulse rate. With the use of a specially designed sample holder 350 samples can be analysed per day by two technicians at the cost of 0.67 DM/sample.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 89-94
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
DETERMINATION OF LYSINE IN GRAIN-MEAL SAMPLES, BY DANSYLATION
D. CHRISTOFFERS, A.K. KAUL and B. GEORGI
Institut für Biophysik, TU Hannover
und
Institut für Strahlenbotanik Hannover Strahlen - und Umweltforschung mbH, Hannover, BRD
Abstract
Chrstaffers, D., Kaul, A. K. and Georgi, B. (1974): Determination of lysine content in gram-meal samples, by dansylanon. - Genetika, Vol. 6, No. 1, 89-94.
A fast screening method for the determination of lysine content of proteins in grain-meal samples is described. The fluorescent dye l-dimethylamino-naphthalene-5-sulfonyl chloride (abbreviated: dansylchloride) reacts specifically with the lysine sites in a suspended sample under defined conditions. Other sites in the protein molecules and cellulose or starch do not react. A correlation coefficient of 0.80 was obtained between the relative fluorescence intensity and the lysine content as determined with various conventional techniques.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 95-104
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
DETERMINATION OF RELATIVE NUTRITIVE VALUE (RNV) OF GRAIN SAMPLES WITH TETRAHYMENA PYRIFORMIS (W)
A.K. KAUL, H.W. KASTNER, B. GEORGI and D. CHRISTOFFERS
Institut fur Strahlenbotanik der Gesellschaft für Strahlen - und
Umweltforschung mbH, Hannover und Institut für Biophysik, TU Hannover, BRD
Abstract
Kaul, A. K., Kastner, H. W., Georgi, B. and Christoffers, D. (1974): Determination of Relative Nutritive Value (RNV) of grain samples with Tetrahymena pyriformis (W). - Genetika, Vol. 6, No. 1, 95-104.
The Relative Nutritive Value (RNV) of 5 cereal and 8 legume samples, with casein as a standard, has been determined. Samples were prehydrolysed with pronase enzyme and the dialysates of hydrolysates fed to Tetrahymena pyriformis. The cells were counted after 72 hours of culturing under a microscope and with the help of a coulter counter. Preliminary data collected on the biomass suggested that such observations would be superior to ordinary microscope counting. The RNV values correlated well with the content of lysine + methionine + cystine in 100g samples. With (further improvements in the technique of measurement it should be possible to get a good RNV index of the plant breeding samples in F4 or F5 generations.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 105-113
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
A CYTOGENETICAL STUDY OF G-LIKE CHROMOSOMES
Vlasta HITREC i Ljiljana ZERGOLLERN
Citogenetski laboratorij Klinike za Dječje bolesti - Rebro, 41000 Zagreb, Jugoslavija
Abstract
Hitrec, V. and Zegrollern, Lj. (1974): A cytogenetical study of G-like chromosomes. -Genetika, Vol. 6, No. 1, 105-113.
In their report the authors have presented four persons with different phenotypes who had one supernumerary chromosome in their karyotypes. Using the denaturation technique 'the authors tried to identify and classify these chromosomes.
Referring to contemporary literature the authors have discussed the possible origin of the accessory chromosomes and the possibilities of their phenotypic effect.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 115-120
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
DENATURATION AS THE METHOD OF IDENTIFICATION OF CHROMOSOMES IN TRANSLOCATION
Dubravka MUŽINIĆ and Ljiljana ZERGOLLERN
Citogenetski laboratorij Klinike za Dječje bolesti - Rebro, 41000 Zagreb, Jugoslavija
Abstract
Mužinić, D. and Zergollern, Lj. (1974): Denaturation as the method of identification of chromosomes in translocation. - Genetika, Vol. 6, No. 1, 115-120.
The development of a new banding technique has enabled us to perform the quick identification of individual chromosomal pairs, as well as the precise localization of chromosomal breaks followed by the rearrangement of gene mass. Owing to this technical progress, recently we were able to discover several variants of reciprocal translocations in our laboratory. The purpose of this article is to report our cases of discovered translocations.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 121-125
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
IDENTIFICATION OF THE PHILADELPHIA (Ph') CHROMOSOME BY A G - BAND METHOD
Milena ĆIRIĆ
Faculty of Medicine 11000 Beograd, Yugoslavia
Abstract
Ćirić, M. (1974): Identification of the Philadelphia (Ph1) chromosome by a G - band method. - Genetika Vol. 6, No. 1, 121-125.
Using a G - banding method the Philadelphia (Ph1) chromosome was identified as a deleted chromosome 22 (22 q). It was possible to determine the position of the band on so small a chromosome by applying the method of comparative analysis, namely, by comparison of the photographed mitosis before and after treatment. This method will be of particular value in analysis of a new clone which appears in some malignant hemopathies.
Published in „GENETIKA“ Vol. 6, No.1 (1974), pp. 127-136
s© 1974 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
Original scientific paper
THYROID AUTOANTIBODIES IN CHILDREN WITH DOWN'S SYNDROME AND IN THEIR PARENTS
Mira TUNKL and Ljiljana ZERGOLLERN
Radioizotopni odjel Interne klinike i Dječja klinika Medicinskog fakulteta - Rebro, 41000 Zagreb, Jugoslavija
Abstract
Tunkl, M. and Zergollern, Lj. (1973): Thyroid autoantibodies in children with Down's syndrome and in their parents. - Genetika, Vol. 6, No. 1, 127-136.
In this study the authors investigated the frequency of thyroid autoimmuniity in children with Down's syndrome and in their parents. Circulating antibodies to thyroglobulin were determined by Boyden's haemagglutination test with tanned red blood cells coated with thyroglobulin. Antibodies to microsomal fraction of thyroid gland were detected by complement fixation test.
These immunoilogical investigations were performed in 240 persons: 54 children with Down's syndrome, 49 mothers, 28 fathers and 2 sisters of index patients. The control group consisted of 82 adult persons and 25 children.
The frequency of thyroid autoantibodies in children with Down's syndrome was 50%, in mothers of children with Down's syndrome 26%, while in fathers the frequency was 14%. In the control group positive results were found in a much smaller percentage (8% and 6%). The investigation is continuing.